LOCUS       DAZUKR010000001        89655 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000001 DAZUKR010000000
VERSION     DAZUKR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.001"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..2165)
                     /locus_tag="ACG89C_000001"
     CDS             complement(552..2165)
                     /locus_tag="ACG89C_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4898597.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="ACG89C_000002"
     CDS             complement(2306..3214)
                     /locus_tag="ACG89C_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4898598.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="ACG89C_000003"
     CDS             3397..4521
                     /locus_tag="ACG89C_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4898599.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4748..5665
                     /locus_tag="ACG89C_000004"
     CDS             4748..5665
                     /locus_tag="ACG89C_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4898600.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="ACG89C_000005"
     CDS             5658..6491
                     /locus_tag="ACG89C_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4898601.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="ACG89C_000006"
     CDS             6574..7692
                     /locus_tag="ACG89C_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4898602.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7713..9584
                     /locus_tag="ACG89C_000007"
     CDS             7713..9584
                     /locus_tag="ACG89C_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS4898603.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9578..10801
                     /locus_tag="ACG89C_000008"
     CDS             9578..10801
                     /locus_tag="ACG89C_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4898604.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="ACG89C_000009"
     CDS             complement(10887..12821)
                     /locus_tag="ACG89C_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4898605.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="ACG89C_000010"
     CDS             13047..13703
                     /locus_tag="ACG89C_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4898606.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="ACG89C_000011"
     CDS             complement(13777..14130)
                     /locus_tag="ACG89C_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4898607.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="ACG89C_000012"
     CDS             14322..15251
                     /locus_tag="ACG89C_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4898608.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="ACG89C_000013"
     CDS             15262..16098
                     /locus_tag="ACG89C_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS4898609.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="ACG89C_000014"
     CDS             16346..17122
                     /locus_tag="ACG89C_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4898610.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="ACG89C_000015"
     CDS             17298..18041
                     /locus_tag="ACG89C_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898611.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="ACG89C_000016"
     CDS             18034..19614
                     /locus_tag="ACG89C_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS4898612.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="ACG89C_000017"
     CDS             complement(19865..20344)
                     /locus_tag="ACG89C_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4898613.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="ACG89C_000018"
     CDS             complement(20360..21112)
                     /locus_tag="ACG89C_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4898614.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="ACG89C_000019"
     tRNA            21229..21299
                     /locus_tag="ACG89C_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="ACG89C_000020"
     CDS             21660..22385
                     /locus_tag="ACG89C_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4898615.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="ACG89C_000021"
     CDS             22640..22906
                     /locus_tag="ACG89C_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4898616.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="ACG89C_000022"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="ACG89C_000022"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4898617.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="ACG89C_000023"
     CDS             23359..23664
                     /locus_tag="ACG89C_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4898618.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23745..24890)
                     /locus_tag="ACG89C_000024"
     CDS             complement(23745..24890)
                     /locus_tag="ACG89C_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4898619.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24951..25598)
                     /locus_tag="ACG89C_000025"
     CDS             complement(24951..25598)
                     /locus_tag="ACG89C_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4898620.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25792..26085
                     /locus_tag="ACG89C_000026"
     CDS             25792..26085
                     /locus_tag="ACG89C_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898621.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26129..26440
                     /locus_tag="ACG89C_000027"
     CDS             26129..26440
                     /locus_tag="ACG89C_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898622.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26437..27061
                     /locus_tag="ACG89C_000028"
                     /pseudo
     CDS             26437..27061
                     /locus_tag="ACG89C_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27202..27360
                     /locus_tag="ACG89C_000029"
     CDS             27202..27360
                     /locus_tag="ACG89C_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898623.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27361..27723
                     /locus_tag="ACG89C_000030"
     CDS             27361..27723
                     /locus_tag="ACG89C_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898624.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27760..28608
                     /locus_tag="ACG89C_000031"
     CDS             27760..28608
                     /locus_tag="ACG89C_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS4898625.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28598..30073
                     /locus_tag="ACG89C_000032"
     CDS             28598..30073
                     /locus_tag="ACG89C_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS4898626.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30339..30746
                     /locus_tag="ACG89C_000033"
     CDS             30339..30746
                     /locus_tag="ACG89C_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS4898627.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30749..30964
                     /locus_tag="ACG89C_000034"
     CDS             30749..30964
                     /locus_tag="ACG89C_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898628.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30968..31348
                     /locus_tag="ACG89C_000035"
     CDS             30968..31348
                     /locus_tag="ACG89C_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4898629.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31482..31637
                     /locus_tag="ACG89C_000036"
     CDS             31482..31637
                     /locus_tag="ACG89C_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS4898630.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31706..32179
                     /locus_tag="ACG89C_000037"
     CDS             31706..32179
                     /locus_tag="ACG89C_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS4898631.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32176..33870
                     /locus_tag="ACG89C_000038"
     CDS             32176..33870
                     /locus_tag="ACG89C_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4898632.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33836..34021
                     /locus_tag="ACG89C_000039"
     CDS             33836..34021
                     /locus_tag="ACG89C_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898633.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34025..35200
                     /locus_tag="ACG89C_000040"
     CDS             34025..35200
                     /locus_tag="ACG89C_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4898634.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35193..36716
                     /locus_tag="ACG89C_000041"
     CDS             35193..36716
                     /locus_tag="ACG89C_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4898635.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36772..37056
                     /locus_tag="ACG89C_000042"
     CDS             36772..37056
                     /locus_tag="ACG89C_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4898636.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37043..37378
                     /locus_tag="ACG89C_000043"
     CDS             37043..37378
                     /locus_tag="ACG89C_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4898637.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37673..38299
                     /locus_tag="ACG89C_000044"
     CDS             37673..38299
                     /locus_tag="ACG89C_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898638.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39041..39878
                     /locus_tag="ACG89C_000045"
                     /pseudo
     CDS             39041..39878
                     /locus_tag="ACG89C_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            39943..40377
                     /locus_tag="ACG89C_000046"
     CDS             39943..40377
                     /locus_tag="ACG89C_000046"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4898639.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40445..41767)
                     /locus_tag="ACG89C_000047"
     CDS             complement(40445..41767)
                     /locus_tag="ACG89C_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS4898640.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41921..43561)
                     /locus_tag="ACG89C_000048"
     CDS             complement(41921..43561)
                     /locus_tag="ACG89C_000048"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS4898641.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43891..44361
                     /locus_tag="ACG89C_000049"
     CDS             43891..44361
                     /locus_tag="ACG89C_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS4898642.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44990..46093
                     /locus_tag="ACG89C_000050"
     CDS             44990..46093
                     /locus_tag="ACG89C_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS4898643.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46377..47057
                     /locus_tag="ACG89C_000051"
     CDS             46377..47057
                     /locus_tag="ACG89C_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4898644.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47147..48181
                     /locus_tag="ACG89C_000052"
     CDS             47147..48181
                     /locus_tag="ACG89C_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4898645.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48195..48530
                     /locus_tag="ACG89C_000053"
     CDS             48195..48530
                     /locus_tag="ACG89C_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898646.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48514..50994
                     /locus_tag="ACG89C_000054"
     CDS             48514..50994
                     /locus_tag="ACG89C_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898647.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51050..52609
                     /locus_tag="ACG89C_000055"
     CDS             51050..52609
                     /locus_tag="ACG89C_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898648.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52606..53970
                     /locus_tag="ACG89C_000056"
     CDS             52606..53970
                     /locus_tag="ACG89C_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4898649.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54141..55211
                     /locus_tag="ACG89C_000057"
     CDS             54141..55211
                     /locus_tag="ACG89C_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898650.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55226..55894
                     /locus_tag="ACG89C_000058"
     CDS             55226..55894
                     /locus_tag="ACG89C_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898651.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55914..56492
                     /locus_tag="ACG89C_000059"
     CDS             55914..56492
                     /locus_tag="ACG89C_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4898652.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56895..57806
                     /locus_tag="ACG89C_000060"
     CDS             56895..57806
                     /locus_tag="ACG89C_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4898653.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58062..58979)
                     /gene="rnhC"
                     /locus_tag="ACG89C_000061"
     CDS             complement(58062..58979)
                     /gene="rnhC"
                     /locus_tag="ACG89C_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS4898654.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59181..59618
                     /gene="zapA"
                     /locus_tag="ACG89C_000062"
     CDS             59181..59618
                     /gene="zapA"
                     /locus_tag="ACG89C_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4898655.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59676..60223
                     /locus_tag="ACG89C_000063"
                     /pseudo
     CDS             59676..60223
                     /locus_tag="ACG89C_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            60338..62698
                     /locus_tag="ACG89C_000064"
     CDS             60338..62698
                     /locus_tag="ACG89C_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4898656.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62785..63099
                     /gene="trxA"
                     /locus_tag="ACG89C_000065"
     CDS             62785..63099
                     /gene="trxA"
                     /locus_tag="ACG89C_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4898657.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63281..65059
                     /gene="uvrC"
                     /locus_tag="ACG89C_000066"
     CDS             63281..65059
                     /gene="uvrC"
                     /locus_tag="ACG89C_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4898658.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65204..66103
                     /locus_tag="ACG89C_000067"
     CDS             65204..66103
                     /locus_tag="ACG89C_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898659.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66096..67358
                     /locus_tag="ACG89C_000068"
     CDS             66096..67358
                     /locus_tag="ACG89C_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898660.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67521..69419
                     /locus_tag="ACG89C_000069"
     CDS             67521..69419
                     /locus_tag="ACG89C_000069"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS4898661.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69718..70437)
                     /locus_tag="ACG89C_000070"
     CDS             complement(69718..70437)
                     /locus_tag="ACG89C_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS4898662.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    70627..70674
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            70774..71106
                     /locus_tag="ACG89C_000071"
     CDS             70774..71106
                     /locus_tag="ACG89C_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898663.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71093..73096
                     /locus_tag="ACG89C_000072"
     CDS             71093..73096
                     /locus_tag="ACG89C_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS4898664.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73115..73585
                     /locus_tag="ACG89C_000073"
     CDS             73115..73585
                     /locus_tag="ACG89C_000073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS4898665.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73606..74190
                     /locus_tag="ACG89C_000074"
     CDS             73606..74190
                     /locus_tag="ACG89C_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFS4898666.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74205..75191
                     /locus_tag="ACG89C_000075"
     CDS             74205..75191
                     /locus_tag="ACG89C_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS4898667.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75181..75492
                     /locus_tag="ACG89C_000076"
     CDS             75181..75492
                     /locus_tag="ACG89C_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS4898668.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75514..77295
                     /locus_tag="ACG89C_000077"
     CDS             75514..77295
                     /locus_tag="ACG89C_000077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS4898669.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77288..78664
                     /locus_tag="ACG89C_000078"
     CDS             77288..78664
                     /locus_tag="ACG89C_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS4898670.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78671..79306
                     /locus_tag="ACG89C_000079"
     CDS             78671..79306
                     /locus_tag="ACG89C_000079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS4898671.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79336..80691
                     /locus_tag="ACG89C_000080"
     CDS             79336..80691
                     /locus_tag="ACG89C_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4898672.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80883..82277)
                     /locus_tag="ACG89C_000081"
     CDS             complement(80883..82277)
                     /locus_tag="ACG89C_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4898673.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82369..83340
                     /locus_tag="ACG89C_000082"
     CDS             82369..83340
                     /locus_tag="ACG89C_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4898674.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83337..84119
                     /locus_tag="ACG89C_000083"
     CDS             83337..84119
                     /locus_tag="ACG89C_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS4898675.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84288..84659)
                     /locus_tag="ACG89C_000084"
     CDS             complement(84288..84659)
                     /locus_tag="ACG89C_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4898676.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84793..85236
                     /locus_tag="ACG89C_000085"
     CDS             84793..85236
                     /locus_tag="ACG89C_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4898677.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85279..86088)
                     /gene="yidA"
                     /locus_tag="ACG89C_000086"
     CDS             complement(85279..86088)
                     /gene="yidA"
                     /locus_tag="ACG89C_000086"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4898678.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86107..87474)
                     /locus_tag="ACG89C_000087"
     CDS             complement(86107..87474)
                     /locus_tag="ACG89C_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4898679.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87458..88288)
                     /locus_tag="ACG89C_000088"
     CDS             complement(87458..88288)
                     /locus_tag="ACG89C_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS4898680.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88451..88888
                     /locus_tag="ACG89C_000089"
     CDS             88451..88888
                     /locus_tag="ACG89C_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS4898681.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88964..89584
                     /gene="rpoE"
                     /locus_tag="ACG89C_000090"
     CDS             88964..89584
                     /gene="rpoE"
                     /locus_tag="ACG89C_000090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS4898682.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        29552 a        15170 c        18471 g        26462 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ttgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagactata aagctaggag aaaccttgat atgatagggt
    23701 ttctcctttt tttatttttt actgtatgcc atattgtatg ccatctagtc aatatttaag
    23761 tgttgtacta atttttcaac agtcttttct ttcgtttctt ttgccaagtg accgtaacaa
    23821 tcgatcgtct gactaatttt tgtatgccgt aatctagttt gaacttctac catagtagct
    23881 ccagcgtcca agagcattga agcatgagtg tgtcttgcta catgcacacc gagaaatgga
    23941 accatagctt tttcacaaat agtaatcaat cttgcataaa tagcgtggtg tgtgattagt
    24001 tctttataga taccacaaaa aatcatagtc ggatcagtta caccaagtgt cagcatatat
    24061 ttccgctgat taatctgcca tttttttaat atagaaattg ttctttcatc tagtggaaga
    24121 accccagcac tacaatcagt tttaggatca ctgattttat agccattttt ggattggctt
    24181 agtgttttat tgatggtgac cgtattttct tgaaaatcaa tgtctgacca attcaatgca
    24241 agggcttcac tgatcctaac cccgctataa aagagaaagc gtagtaacgt tttatcaaag
    24301 tcgtttcgat aagtttcagg tttgctgttt aggtaatcaa acaaggtgat cagttgttct
    24361 ttactgtaaa ctttgacctt ttctttatgc ttagtggtgg ttcttttagg cattaatacg
    24421 ttgtctagtg gattagtctc aataatccca agattgatag cgtagctaaa aatacgctta
    24481 gaagttgaat gaagtacctt gtattggtca gtagtttcag cccagctatt tacaatttgt
    24541 tgggcttttt ttaccgtgac ttcattcaca cgaagatttc catattttgg aagaatccac
    24601 tttctaaact tgatttcagt tttcatgaag gtggcttctc ggacagtggt tttatatgtt
    24661 tctagccaca agtcataaac ttctttaaac gtttcgttgt tcgctttttt aataccgttt
    24721 ttatcgtagt ccacttgcaa gcgtgacaag gcaagctggg cttctttttt tgttttgtaa
    24781 cctcgtctac gaaccttatc aggtttgcca gtcattttgt tcattccaag ataggcttca
    24841 tagagccaaa atttaccttt tttagtttca tattgcttga atgttgccat aatagttttc
    24901 cctccatagt gtagcaagcc gtgggggcgg ttggaggagg cagtttatta ttattttgaa
    24961 tagtgtttat ttagctcagt aacaagagct gtgaactctt ttagaagatc attttcataa
    25021 aattcttgat actctttttt ctcatctgta tttttttctg aatcattaat taatgagtca
    25081 tatcgtaaaa gcgtatttag aagagctgcg agataagcaa ttggggtact cccgtcagga
    25141 aattcaattc gagatcctgt tttaagatat tttattgcgt ttgttaaata aatcttctgt
    25201 gagacagtca ggttatcttc ttcgatgtta ttgatagcat tagaaagatt agaatcaatt
    25261 atagagttat ttttttcaat agtttttttc aattgctttt caagcacatt tcgcttaatt
    25321 tcgggcatca tatgcatatc tcttaatgta taagatccgt ttaatagata ggtagtggat
    25381 acatctaata cctttgaaag ttgtttaagc ctggtgttat tgggaagatt atagccattt
    25441 tcccaattac taacggcacc tttactggcc ggtggggata atttacctcc aagttgttcc
    25501 attgagagtc ccttttcaat tctagccagt ctaattcttt gtcctgtctc cactgcttcg
    25561 gggtattttt ttctagccac attttcacca ccattcatat attttctcta ctatatcacg
    25621 atttaaatat gtgtgaaaca taaaagttca aaaaagtatg ttaaaattct tgcaaatgaa
    25681 aaggtatgat ggtatattga gtttataaga ggtatagaaa tgtattcaag agcctcttgc
    25741 atttttttaa aatctttgta tagaaatgta tgctaacgag gggggataaa tatggaatta
    25801 aaaattgaca aactcacgct tgatgatcca gcttttaaaa ttattgacga tcgagttgtt
    25861 caacaagtag agcaaactca aggaacgtta ctttcattat tagaaaaaaa gtaccagttt
    25921 ccaaaatata tgaacaaggc acaagctgct aagtatatga acgtttcata caatactatg
    25981 atgcagaaat atgttcctaa cggtttaaag ttgattatag ttgatggagt cgtaagaatt
    26041 tcacaagatg aatgtgatcg ctttatggaa gaataccaaa aataaacgca agccgtgggg
    26101 gcaaagggat taataggagg aaataataat ggaatcgaca caaaataaag caatcgaaaa
    26161 agtattatca acaattctta cagaagacac agcggcagaa ttagcaaact tggaaggcaa
    26221 agcgctagaa gaaacgtttg aatgtttgta cgaacaaatg gactatcaaa aattgctacc
    26281 acaagaacca acggtaagcg gagtattgcg tggcttgaac gatttagtac aagcagagtt
    26341 taaagaacga ctttctattg aagagtatca agaaattttg tatcagcaag ttgagttgtt
    26401 agcgagcttg ctagggcttg aactggagga cgttgaatga aaactgaaat ttggaacgga
    26461 cacattataa gatttgtcga tattaatgat gaatggtggg cagtggcgaa agatgttgcg
    26521 gaagcgttag gtcttaaaca ggttactaga gccattcact ctttgcctaa agatggggtt
    26581 actactagta aggtcatcga ttcattgggc agaacacaag atgtaaacat catcaacgaa
    26641 aaaaaatatt taccgcatgg cattcaaaag ccgtaaaaaa gaagccgagg catttcaaga
    26701 ctggattttc gacatcatca aagaactacg ccagtccaca ggacttgaag gctttcaagt
    26761 attccgcatg ctagacaaag aacaccaaaa agaagcaatg actaaattaa gccacgctat
    26821 tactgaacca aaaccagtag actacattaa agcgaatgtg atagccaata aagctgtatc
    26881 aactatttat ggtcattcca aaatggtaaa aaagaaagat atgactcctg aaatgttggt
    26941 tgatcgtgaa cctattttag atgagacagt agaattaatg actgttaaag aaaaatatgg
    27001 gttgcagttt tcagtgagtg agaaaattta taatcgttct gctgaattgc agacaactta
    27061 ggagcattaa catgatcgag aacaaagaaa aagccttatc cataacagtc ttggcggaca
    27121 ggataaaggc aacttaattg ggataaaacc cgatattctt tgttccgatt ataccatatt
    27181 aaaaaatcgg agggaaccat tatgaaacga aacttaatta taggaagcat tttaatcggc
    27241 ggaattttcg gcttattacc attaattgca aaaatagcta tctttgcagg attagcgtta
    27301 tatctcttca ctgcttatga cgagtacgac tatcaattaa gaactggagg gcataagtag
    27361 atgtacaaaa caaaactatt aaaccaactc gatagccttg aattagagga aattaatcaa
    27421 gggatcgctg aactagaaaa taatatcggg aaaacctact ttggaaattc attcaatgaa
    27481 aaactaacag ttttgtatgt actcaaaaaa catgcagaac acaaaataat ttgtcgagaa
    27541 atcaacgagc taaaaaatca gattttaacc gcatggttaa acattactga tatgcaagaa
    27601 gcaagggtga aaacatttaa tacatgggta aagtatcaga atcaattaaa aggtgctgaa
    27661 tttgttcggg atgggttgaa atacgaactt gagcagttga agctcatgga ggtgtctgaa
    27721 tgatttatac cagtattttg aaaaaggagg agaggtttat tggcaaataa ttatcaagca
    27781 gcaacggaat tacaaaagtc gggatggtca atttatccct tatctcctgg aactaaaatt
    27841 ccaataaaag gctctagtgg ttggaatgat gcaactaacg attctaatca aattaatgaa
    27901 tggtggacgg aagatgcaac tagaaatatt gggttaatgc ttgaagatca tcaaatggtg
    27961 gtagttgacc tagatcaaca ttctgaaaca cgaaacggcg ttgagaatta taaaaaactt
    28021 gcttcaatgt atgatccatt tccttctact tatacggaaa tcacaccagg taaaggaatc
    28081 catttcttct ttaaaattcc tgatggtgta attatccaac aagaaacagg tgcatttgct
    28141 gatatcttcg ggcgtgatga taaaggtaaa agtttaagcg ggattgatat tatcactaaa
    28201 ggagttccaa tagcgccaac tatgattatt tcaagaagtg ttgggagagc atatgaacct
    28261 attgaaaacg tatctctaaa taatattgca ccagctcccg attggctagt gaacctactg
    28321 ctaaaaaaac cgcaagaaat aaagactcaa taccgacccg tagcaaaaac agggacagca
    28381 aaaaaattag atatgattgt tcaaggcgct ggcgaaggta gtagaaacga tcatttaact
    28441 aagttatgcg gttggttact atggcacggt gtagataatc aaactttagc agaattgatt
    28501 tatacagcaa atgcgtacaa tgaaccaccg ctaaaagata aagaagtgaa tcaaattatt
    28561 cgatcgatga ttaaaaaaga tttgaggggg aaagctcatg gctcataagc ataaattaga
    28621 tagtatttta gactttcccg aagccagcga gcgtgaagac aatatcattg aattgaaaac
    28681 ctggatgtca cgattaagat gcaataaaga cgatcagatt aaaagtaaca gtgttgtgaa
    28741 tgcagaattg attttaacca atgatagcaa tttagctgga acaattgctt ataacgaatt
    28801 tagcggatac attcacctat taaaagattc tccttggatc aatcgtaccg ctggagaatg
    28861 ggaagacagt tttgaggatg ccttaacggc atatattgaa gaaaactaca atgtagtttt
    28921 tgatgacaac aaaatacaca aagcagtagt aaatgtggct agaaagaatg ttttcaaccc
    28981 agtaaaagaa cgtatcgaaa aagttaaatg ggatcaacag cctagactgg aaacactgtt
    29041 tattgacctt ttaggggtag acgacaacct ttacacacga gaagtaacaa aacgttggat
    29101 agtcggatca gtcgcacgta tctataaacc aggaataaag tttgagatcg ttccagtttt
    29161 ggatggaccg caagggattg gtaagagtac cgttccagca ttactttaca cggatgattt
    29221 tttcaccgat tcattagatt cgctaggtga aaagaaagat gattatatgc agcttcaagg
    29281 caatgtaatt attgaacttg gagaattatc ttcaatgaac aaaactaaaa ttgaacagat
    29341 aaaaaacttc atcagtgcca agatcgataa aattagacct ccttatggtc ggaatgtcat
    29401 agcttgggca aggcaatgtg tatttattgg aacgtcgaat gatgggcagt atttgaaaga
    29461 tgatacagga aaccgccgtt tttatccttt gccttgtaag aataagccaa agatgaatcc
    29521 gtttaaaaca aaggatgaat attttcttca agtgttagct gaagcaaagg tgctttttga
    29581 taaaggacaa cgaatctatt ttagtcccga tgaagataaa gaagtcttag aaattgcaaa
    29641 agactatcaa gaggatgcca agatggagaa tccaattaaa gaagcaatca ctaaatattt
    29701 ggaaatggaa atcccgtata aatgggaaga agctccagcg tgggcgagac gttcttatta
    29761 tcagaactat cctgacagta aatgtaatga gaaaaattta cagcatttct ataatggagc
    29821 tactatgcaa caagagtttt atttagttga tagcgtactc accgctgata ttttggaagc
    29881 tgtttttgat aaacaagcaa aggatttatt gaatggtcga tcagatgcag aaactaaaaa
    29941 gattgccttg attattagca gtattcctgg ttgggaacgg aagcaactgc caaaaagaaa
    30001 taaacgacga ggattttaca ataagtcaaa tgcctatgaa aataaaaatc gagctggaaa
    30061 gtacaaaaag taacgggtag taacgggtca gtaacaccta gcccgttact agtttaccct
    30121 tactgcccca agggatacag caatttggta acggggtaac accttatttc cttaaaactt
    30181 ttgaaatagt tagctaatat gctggggaat aatgttactg gattttaccc gttacctgtt
    30241 accaaaacgc tagaaccctt gtccccgttg ggattgagtg gtaacgggta cctgttacgt
    30301 aggtgttacc acgtgttacg aatcaaggag gaaagtaaat gctcaatatt atttcaacaa
    30361 acaaagcacc gaactttcaa tatacagatg aaatggatcg gtttctaatg aacacgctgg
    30421 catttagtgt gggattggta acagaagatt attccacatt tgatccagaa gtattaaaga
    30481 taatggaaga agaacctgat tggctgcagg agtcagtagc gtggtgtcaa tcgctagtag
    30541 tgggatcatt ggtagatagt ggcaactatg atgatacagg tgagctaatg gatgaattta
    30601 attgtctgct taacctatat gatcgagcga gacaacgaga gcttacatcg aatgaggata
    30661 atttgttttt aaacatccat gataagttct tggctttgct actaacagat gatgaattga
    30721 taactaattt attggaggta gaataattat gcatatggaa agtattagat acttcgatgg
    30781 tgagaaacac attgagtatt caaacactca agaagatgta gactttatta gttttgagga
    30841 agataaaatc actaaagtta attttaaaga cggaacgtgc ttgaagatag tttcgcctta
    30901 tattgaatac aaatcaaaat gggaacaaga tgattctgac gaacgagatc gctttggttg
    30961 gtaatctatg gcagttaaaa aacaatgtaa tcatgctgga tgtaaaacat tgattgatta
    31021 taggcagaag tattgtggga agcataaagc aaagcaaacg gcaattaagc gtgaagaaag
    31081 aaagcaaagc gaaggtaaat attttcaatt ctatcaaagt agaacatggc gaaaggcttc
    31141 atacttgtat cggttaaatc atccagtatg tgaggattgc ctagaagaag gcttgataag
    31201 gaaagctgat gttgtagatc acaaaattga actaaaagat gattggaata gacggttaga
    31261 tgaaactaat tttcgttctt tatgccatgc acaccataat tccaagacag ccaatgaaag
    31321 acaaagacga gaaaagagta ccctttgagt taagggggca tagtgtgaaa cttactgaca
    31381 atcgatgcct actcattttg gtacaaataa ccgttgtgaa aaggcataag ggtaattaca
    31441 aataacgatt acacttgtaa tcacaagtga aaaagtatat aatgaaagta ggagatttat
    31501 tgaaaaatac aattgttatt actgataaaa cagggaaaca gcgcaccatt gagttacctc
    31561 attttggtca agtgaccatt caaatgcaga acggaaaaat catttatata gataaattag
    31621 ataaagaaaa attctgatcg aaaaacgaag gaatgtgagc ttaattgctt acgttccttt
    31681 ttcttttgtc tgaaaggagg acaacatggg acaccctaaa atattacacg atacaaaagg
    31741 caatatatca agtgaagaaa aagcaatccg tgtggacgct agagaagaat tgttcaagca
    31801 gcaaccatta ataaatatca cgccccctga ctggatggca gcgagcgcta gaagtgaatg
    31861 gaatcgtatc gtaccaacat taaaaaaaga ttatccattg agtgaagcag attatggttc
    31921 attggtagcc tattgtttag cctttgctcg aatgaaaaca gccgaagccg agataagaaa
    31981 atcaggtacg tttatcacat gcgaaaacgg agtaaagaaa gccaatccag cggttcgggt
    32041 tcaatcacaa gctatgagtg atttgaaaaa acaagccacc tcactaggta tgaccttaga
    32101 atcacgatca aaactagctt tgaacaaggt taaaaatgat gaacccgaag acccgttcaa
    32161 agagttgatg aattcatgaa tgattacatt gagaaagtcc tatcaggtaa gctgattgca
    32221 cccaagaaaa ttatccaagc gtgtgagcgt catataagcg atttggagcg ttctaagtca
    32281 gatagcttcc cttatgtgtt cgatgaagaa caagccacca aagcgattaa attcattgag
    32341 ttgctaccat ctacggacgg taaagcaatc aagatgttag gatttcaaaa attcattcta
    32401 ggcagtcttt atggctggcg tactaaagaa gggaattaca ggcgattcaa tcgagcgttt
    32461 accagtatga gccgtaagaa tgggaaaacg tatatcgcaa gtggcatggc tgccaatgcg
    32521 ttgattatgg aacaagaacc agcagaagca aggcaagtat tgtttgtaag taacgctttg
    32581 aaacaagcta aattgggcta tgatatgctg tctaattcac ttagaaacgt ggtcaagtct
    32641 agtaagtttt taagaccaca actgaaaatt atgaactcta agattcagca cttgccctct
    32701 aattcgttcg ctatggcact ggctagtgaa accagcacgc tagatgggtt tgcaccaaca
    32761 accgcaattc ttgacgagtg gcacgaagca aaaactcgta aaacgtacaa cgtcattaag
    32821 tcaggaatga cccaacaaaa gaacggctta ttgtgtgtta ttagtaccgc tgggcttgat
    32881 ttaaacgttc ctatgtacga ggaatactta ttgttagatc gtgtgctaaa aggcgaagaa
    32941 caagccgaca ggtactttat agcgatatgg gaattggacg atcccgaaga aattcacgat
    33001 caagagaaat ggatcaaagc caatccgatt tttgaaagtg aagaaatcaa aaaagtaatg
    33061 attccaacca ttcaagatga tgtgaacctt gctttgaaac aaaataacct taattctgta
    33121 ttggtgaaaa acttcaattt atggagacaa gcgagtgagg acagctatat gattgctgaa
    33181 gactggcagg caaccgaagt agaaccacaa gatattacag gcaagcccgt ttatatcgga
    33241 gtggatttat ctaaaacaga tgatttaact agcgtttcat ggatcgtacc gctagataac
    33301 ggcgaacttt attgtgattc acatagcttt gtagccacca aatatgggct tcaagacaaa
    33361 gaaaagcgcg acggtttgcc ttatcgagaa ctagaaaaag ctggtgagtg ttccattact
    33421 caattagaaa gtggaatcgt ggactatgac caagtatttc agtttattca agatttgatt
    33481 caagaaaatg atttggaatg tatggggatt tgttatgacc cgtataacgc taattcgctt
    33541 atcagtaaag ctgaaaaagc caactaccca atgttagaag taagacaagg aacgattact
    33601 ctaaacgttc cgacccgaac ttttagagaa caagtttatg aaggcaacgt tattcacaat
    33661 aaaaatacga ttctcaccca tgcagtgaac aacgctattt taaaaacgga taacaacggc
    33721 attcagatta ataaatcaaa gaacagtaac aaaattgatc caatagctgc attaatcaat
    33781 gcctatgtgt ttgcaatgga ttacttcacc acaacggaag gagcgaaagc agacaatgaa
    33841 ttttatacaa gtgaagaatt ttctttctaa ttacattcat accgttcttt tacttcttgg
    33901 attggtgtgt gtgttggttg ctatcacctt actaacaaat gtctactatg gcttgttagc
    33961 gctgggcata gtgcttattg gggtagcggt catgctaaat acagaacaga agggaggtta
    34021 aaagatggca ttttttaaag cgagacaaaa tacaacggga gatcctttcc tacaacatgt
    34081 ggtatcaatc caatcggatg attacaccac cagctttaca agcgttcgtg cattaagaaa
    34141 tagtgatgtg tttgcagccg ttcgaattat tgctagtgat attgcttcaa gtccgattca
    34201 attggtcaaa aacaatatgc cacaagctga taatgaactg gtgaagttac taaacgagaa
    34261 acctaattca gaaatggatg gttggcattt aaagtttgct ttggcggtca atatgttgct
    34321 aaacggtaat agctttgcag agattaaacg caacggtgaa aaggtagaag aaattcactt
    34381 attacctaac tcaagtgtaa cggttactca attagataat ggtacgttgt cttatcagat
    34441 tggtgataaa aaaagacgtg tgaagtctag cgatattttg cacttcaaat atttcaccca
    34501 agatggttta acaggattac caccacttta tgcgttacgt gatgaactga aaattcaaca
    34561 agctggcaac cgcacattgc acaatttctt tactcgtggt gtcagtgggt caggtatttt
    34621 gaaggttcat aagtcagatt tagacggatc agcaaagaac gcaattcgtg aaaagtttga
    34681 agaagccaac ggttcaagta gtggagataa tgctcttaga acgatcattc ttgatgaaac
    34741 aatggactac aaaacattag aaataaacac cgatgttttg aagcttatca attccaatga
    34801 ttggaacaca aaacaaattg ccaaggcgtt tggtgtaccg attgagcgtt taggcgttga
    34861 aaatgaacat tctagcaccg ttcaaagtaa tctccagtat atccaaagta cgctgattca
    34921 ttactttaat gtgtttgtga gtgaattaga tacgaaactt gaaaccaata tccgctttaa
    34981 ttccgatcag ttactagaga ctgatcctga aacaaaagta aaaaatgtat tggatcaggt
    35041 caaagggtca cttctcacga ttaatgaggg gcggtcgaaa atggggctac cccccatgga
    35101 tggtggcgat cgtttactag caagtttgaa ctttacgtat ttagatacgt tggagaaata
    35161 tcaattaaaa gaacaggaag gagttacacc agttgaataa tgaagaagaa aaggaaaaac
    35221 ggctgacaga agaagctgag ctaacagccg attctcccaa agtagagaaa gaaaatgaaa
    35281 aacaaccaac agacggcaaa actatttcag gctatgcgtt gaaattcggg caaccgtcaa
    35341 aagatttagg cggctttgta gaagtgatta cacccgaagc attaaaagag gtggatttat
    35401 caaatgtgtt cttattatat ggacacgatt attcaaagcc actagctagt gtcaaagcag
    35461 gtacgttgaa attgaatgtc gatgatacag gattacattt tgaagcagag ctaaccgaca
    35521 cgacctattc aaatgatgtg tatgagaata tctcaaaagg ggttgttgat tctatgtcct
    35581 ttgggttcgt gcttggatta gattcattcg acaaaaaaga agatggcaca attgaacgat
    35641 caatagataa aatcaaagca cttaatgaaa ttagtgtcgt gaccgttccc gcttatgatt
    35701 catcaaatgt ccaagtaaat aagcgttctt acgaatcgtt tatgagtaac aaccaagcaa
    35761 aacaaacaaa caatagctta gaatccactt ctaaagcaca aaaggagagt aataacatgg
    35821 aaaaaacttt aatcgataac gaaaaaacag aattgcgtgg gtatgaagaa tatatccgtt
    35881 cacaaggcga agtgcgtgat ggagtcacta ctgtaaatgc agcggcagtt gttcccgaag
    35941 aagtaatcgg tgaagtcttt gatttgaaac gttcaaatta taacttagct caatatgcaa
    36001 cagtaaaaac agtatcaaat ggacaaggta aatatccagt agcaactaac caacaagcag
    36061 tgttagcaac aaaagctgaa cttgctgaaa ttggtgatat tgacgctgaa atgtttactt
    36121 cagttgatta taaagtagaa actcgtgctg gtaagattgc cttatcaaat gaggttgtgg
    36181 aagattcagc agtgaatatt gtacaagagg tcaaagatca gttagcgaaa ttggtagaaa
    36241 acactgacaa taagcatatc atggatttat taaaaacatt cactaagaaa acggctgcta
    36301 cgttggacga tttgaaacaa ctatacaatg tggcattaga cccagcatta aataaaatgg
    36361 taattctaaa ccaaagcgga tattaccacc tagatacatt gaaagattca gatggacgtt
    36421 acattttaca acccgatgtg acagcaccta gtggtaaatc attattcggt atgccagtag
    36481 tattgattgc agatacattg tttgccaatc ctaaagctgg aacattccct atgattatgg
    36541 gggatattgc acaatctatc tttgttgctc gtagaaatca agtaacgact caatgggaaa
    36601 aattcgatta ctactcacaa ggacttgcag tgatcgttcg caacgattat aagaaaattg
    36661 atcaaaatgc ttcagtgtat attgagttta cgccagttgt aacaccaaaa gtatagaaaa
    36721 atattgggcg gcggtttatc ccactgcctt tttttcatta ggagagataa aatggtaatt
    36781 ttggatagca taaaaaaaag tatgcggatc gatcacacta ttgatgatga ctttattcaa
    36841 caattgattg atacagcagg cgaatatata aaaagtgcta ttgatagtag cgcaacggat
    36901 aaagatatgg ataattatca gcaatttgat ttggcggtgt cattacttac tcaacattgg
    36961 tatttgaacc gtcaagaagc cagcagcgaa cggataccag taacggtaca agcattagta
    37021 caacaaatga gaggtgctta ttatgccaat cattaagaat gtaaatgaat tgactgagag
    37081 agtcaagttt aaaaagacaa aacgggtaaa agatgaagat gggcagatgg tggatagtga
    37141 agaaactgta tttgaatgtt gggctaatgt gcgttcacaa atgctaaaag acgtgcttgc
    37201 tagtgtaggt accattcttg aaggaacgtt gacgtttatt attcgatacg atcaagatta
    37261 tgaactaaca aacgatatga aagtagcttg gaaaaacaag agttataaaa ttatttcaat
    37321 caatgaagga acggcgttta aagattatac aacgatcata gctaaacaga tttcttagaa
    37381 tgattacact tgtaaacgtt gtgggtattt agtataatta aggtagtaaa tgaagggtta
    37441 gctgcctgaa ttttgcaaga cttagctagt cgaaatctat tgttggactg aaaattgtag
    37501 gtgtggttgc aaacatgctg gactaagaaa gatgatagat gatgaacgtt caattttaat
    37561 acaggcatgc taggactgat tggatggttt tggcaagctg attgtgtaga aatcaaagaa
    37621 cacgtctgtt gtggacgtgt tttttttgtt ataattcaaa agaggtgatt taatggacaa
    37681 agcagagttt acaaattttt attggaagta ttatctacat ttagaaaatg agtttattaa
    37741 taccacagat tttgtgatgc tcgatgagaa taatttcaaa acattctcta ttgaatatca
    37801 aaaactacta ctagcgattg gttcggagtg tgagattatt ttcaaagaac tttgcggatt
    37861 tgatagcagt agcaataaaa gcataactga ttttaaagct gttattaaat ctagcgaatt
    37921 aatgtgtttg gataatggtg ttagggttat gaattcttta acattgactt cactaaaacc
    37981 ttttggagat gggtggcctg aagatacacc agattggtgg aaaatatata acagagtaaa
    38041 acatggtcgt tcttcaaatt ataaggaagc aaatttggaa aatgtgttgt atgctttagc
    38101 aagtctatat ttattagagg aatatttgca taagaaagta atactagaag gtgaagtaga
    38161 ctttataagt ccagagtcaa atctatttac gttatcttgg gaaagaaaac attcgtcgat
    38221 gcaaaaagtg acgtttgaaa aagtggattc aaattatgta gctccactca ttgattttaa
    38281 agaacaagaa gaaatttaaa aacccccacg gcttattgaa gtttttgtat gccatattgt
    38341 atgccatttg gtttaaatcg cttgaaaaat agtgatagat aactctaatt catttagtca
    38401 taaacgctgt tttgataacc tttatagttc tttgatatgg gtttctcatt catggcggaa
    38461 gaagaagaat agaacaaaag aatgaattaa taagatataa aaaaagggat tgtgacaaac
    38521 gtcttgtcac aatccctttt tccaaataaa cgatgttcaa tcagctgccc ctcggcattc
    38581 gctcgaaaaa cggcgaaata tgaagaacta ttttgccatt tgtttctcta ataatcaggt
    38641 caaaacgttg acttcacagt ctcttcctta aacggtgttc tcctgccaac tcctcggtca
    38701 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg
    38761 gagctgaacg gcaggctcac gacctctttc ttaaacggtg ttcaaaagct tgcatctgcg
    38821 gtaataagtt caaccaaacg aaaattatga ggagctattt ttgtttggct gttcttatta
    38881 cttgatgcag gacagctttt tcacaacctc ttttcgttta gcactaaatt tccttaaaaa
    38941 gtagaaaagc tttcgaagcc tcgtttgttt cttttactgt ataataaaag aaaagagtaa
    39001 caatcgctaa aagatataaa caagagaaag gaaatacaag atgattggtc tgatttattt
    39061 tgtggtgatc gttctggcga acacagttgg cgctatttcc gggatgggtg gaggcgtgtt
    39121 gatcaaaccg attcttgatc ttatcggcgc ccattctgtt gcagggattt ctttttactc
    39181 aacagttgcg gtattcacga tgtccattgt ttcgactgtg cgacaagtat caagcgggaa
    39241 atcactgaat tggcagattg tcggttgggt atctagtgga gctgttgttg gaggagtagt
    39301 cggcaacatt gtattcgaag tttttttaca gttatttgaa aacgagaagc atgtgcagct
    39361 gatccaaatt tttttgacgg tattgacctt ggtttttgcg ttcttctaca ccaagcatca
    39421 tcagccaaaa tttcatttga cgtcatggac ttggtacctt atatgtgggg ggggttctgg
    39481 gctttttagc tagtttttta ggtattggtg gtggaccagt caatgtttct ttgttgatgc
    39541 tgatgtttgc attgccaata aaagaagcaa cactatattc tctatctacg attttctttt
    39601 ctcaactggc aaaattggta acgattgctt taacaagtag ctttatgcgc tttgatttat
    39661 ctatgttgtt ttatgttatc cccgctgctg ttattggagg tttgtgggga gcaagattta
    39721 gcagggtttt gtctccaaaa aaagtgacct ttatttttca agtaattgtc ctcgtggttc
    39781 ttttggtcaa ttgttacaat gcctttgtca tcttacaaac attaggagaa tctagactaa
    39841 cgcaagtcaa aatcactgaa acatattgtt ctaaatgaaa aaaagagtat actatgataa
    39901 aaaaattcat tttaaaaaat cagaaattgg ctggtgattt ggatgttacg ggaaatgcgt
    39961 ttagaagatg cagaagcact acgtgaaatc aatgcggatc aattagggta tgatatacca
    40021 ttaaccgtta cggtgagaca aatgaagaaa ctgctgaagg agccagagaa aaattttttc
    40081 ttggtttatg aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat
    40141 gaatcgattt ttagcgatcc aatgttcaat attatggcac ttgctgtgtc taaatatgct
    40201 gaaaaaagag gcatcggaaa ggctttgatg actggcgtag aagaagaagc gaaaaaacga
    40261 ggaataacga caataaggct taattctgca gaatacagag tagaagcaca ccaattttat
    40321 gaacacatcg gttatcattc cgataaaatg caaaaaagat ttttgaaaat cctgtaaatc
    40381 tttatcaaaa aaatacccga tcaccaagcc gatacttgat gatcaggtat ttttgttttt
    40441 ttgactaaga aagtaattgc atcaagaaac cagagatgat caaaacgatg gcaccaccaa
    40501 gtcgattgcc catttgagca aaagcaataa gttccatccg attagctgca gataagacgg
    40561 ctacatttcc tgttccgccc atcgagttgt tgcataagcc agcagtaata gttgattcaa
    40621 ttggatataa accaaacaaa cgaccgacaa atcctgaaac aactgaaatc gcgataacac
    40681 ttgtcagaca taagacaaca aattgccatg tcaaagcaga agctaataca ttcagattca
    40741 gcaacgcaat accgataccg gcaagaacag ctgctgtcaa gtttttgaca atcaagttat
    40801 tgaacatgat agctgcatct tcataatact ctggtaatag gttggcgatt ttacagatga
    40861 caacgataat aatcataaat gcgtaagcat ggactttagg aacaaaatag ttaccaattt
    40921 gtcctaagat gaagaaagtc aatgatacca tcaaaccaac acctaactgt ggaatggaag
    40981 gtttcacagt tcgaggtttt ccatcgccaa gatcgccttt ttccattaat ttaccattgc
    41041 cgttgaattt tggcattgat tgtccagcac gagcaactaa tgctgcaccg atgatagcta
    41101 agacatttgt cattgctgtc gcaggaatca ggcgggaaat gatagaagaa gcatccgtac
    41161 ctaaaacatt tgcataaatc gttgataaag gaacggctcc ggctccaaca ccaccagcca
    41221 tcgcaggtaa tgagatgtat aaaacggaat ttgcaaaacc atttccgata agcatgccca
    41281 cgagtcctac ggcaaaaaag gagacgacca ttgaaataag tgcaacggga ataaaacgaa
    41341 cagaagcttt catcaataga ttacggttca taccaaggat gctaccagta atcaaggcag
    41401 caatataaaa gtcccaaaag cccatattgc tgacaaagtt ttctgtggat ttcactacat
    41461 attcaggtaa gatcccaaat gtagcaatcg cagcagaagc gaagatcgca aaaacggaac
    41521 caccacccaa ataacttttg acgataggga tttttgtccc gataaaatga aataaattac
    41581 cgaaaataac caaaactgcg attggtccaa tcatattcgt cggtaatttc cctaaagcca
    41641 ttgcaatgat caggacaagg accatgatca aataaagtgg caaacttacg ccggtgattt
    41701 ttgtggacca aaaaccttct tttttctttt cctcaactgc agttgcagtt gttgtttttt
    41761 gtgccatttt tacagatcct ttcttattct gtatcatatg aaaaaagcca aagaaaagcg
    41821 acaaaagagc aggagttggc tggccgctgt tttgccgcag tcgctttttt ctttttagac
    41881 gtcatacaaa gtttctgtat gttcttttac tataacgatc ttaatcttct aaagatttat
    41941 attcaggaac ccatttagca gattcaactg cttctttgat atccgtgatt tcttcacgat
    42001 tcaagttttg tgcgacaact gattttgcga cagtttcagc aatcgtttga gaaaattcag
    42061 tgatttttgc tactggaggc aagatagctg ctcctggttt tgttacatca acgattccgc
    42121 ctaatgctcg actagcttga gaaatgattt cagcatttac tcgtgtagct gttgaagcaa
    42181 tcaaaccaag tcctaaacct gggtacatca aagcattatt tgcttgaccg atttggtaag
    42241 ttacaccatt atattcaaca tcagcagcag ggatacctgt tccaactaat gcttttccgt
    42301 ccgtccattc tattaaatct tttgcttttg cttctgccaa ttttgttgga ttagacaaag
    42361 gcatgatgat cggacgtggt gtatgagcag ccatttcttt cacgatttct tcagtgaatg
    42421 ctccaggttg ggtagaagta ccaatcatga ttgtcggatg gatcgctttt acaactgctt
    42481 ctaagtttgt caattcatca gcattagtga aatctgagcg tttgcgagcg aatgggattt
    42541 gaccaggagt caatccttct gtatcttcaa acaataaacc ttgtttatct acttgataga
    42601 aatgttggcg tgcttcttct tcaggtacac cttgacggat catctcatct aaaaggatgt
    42661 ttgcgatacc cacaccagca gtaccagcac caaatgtcaa aattatttga tctttcaatt
    42721 gttcaccgga tatattcaat ccgcctaata cacctgctag gacaacaatt cctgtacctt
    42781 ggatatcgtc gttgaaagta gtgatttgat cttcgtattt ttctaagatc gctgcagcat
    42841 ttcctcgtcc aaaatcttcc caatgcaata acaattctgg gaataattcg gtagctgatt
    42901 gaacgaattg atcgatgaat tcatagtaag tttcgccttc tacacgagca tgacgattgc
    42961 ctaagtataa gggattattc aataattctt cattattagt accagcgtcg atcgatacag
    43021 gcaaaacttg tgctggatta ataccagcag cagcagtata gaccatcaat ttgccgatag
    43081 cgatgtcaac accgttcaca ccccagtcgc ccatacctaa gattccttct gcatcagtca
    43141 caacaatcaa acgaatgtct cgtccatctg ccgcgttctt caaacttgct ttgatgtttt
    43201 ctggttcgtc gattgaaaga aatgcagcat cttgcggttt caagaaaatt tcgttgtatt
    43261 gttcgattga atccgcaacg acaggatcat aaacgacagg catgaattca actaaatgtt
    43321 cgcccatcaa tttataaaac aatgtccgat tcgtattgaa tagattcatt aagaagatac
    43381 gtttttccaa atctgtttgt ttgcttaagt attgaccata agcttgaaca gattgttgtt
    43441 ccagtgtttg gacagtactt ggaagcatac cagtgattcc atattttgcc cgttcttctt
    43501 ttgtaaaagc tgttccttta tttagaaaag gattatttaa taagtttaac cctgtaagca
    43561 tgaatgattc ctcctaagtt ttaatacaaa cgaagtatag aagtcagatg atgatatagt
    43621 caaaaagagc aggtatgata aattttattt atatgtaata tagccaaaag aaatcaaaat
    43681 agcagtattt gttcttttta tatcgacaat ccttgcttga atactatgcg tatctcttat
    43741 atgctagaga aagaaaagta gaagaataag ggatagatgg aatggataaa taggatttct
    43801 gaggaaaaaa gcacatgata gcgttgatag gagtcaccta tgtcaggtga taaaaataca
    43861 taagtagaaa attaagagga ggaagagaaa atgaaagaag ttcaattgat tagaaaaagc
    43921 gaactatcag aaggcggttg caatgcatgt ggcgtagtag aagcgacaag ctatacatta
    43981 aaattagatt ccaatcaagc aataatctct gaactgacag taggcggtct tgtggacagt
    44041 ctggcgcttg ctgaaggttt tactggagaa gatatttatg agatgttttc agaagtccgt
    44101 cagttaaaaa aaggagaaaa atgcatagag gttcatcatg agagcccgaa tgtgaggttc
    44161 aaacgtggtg ataatgagat gatcttcaag aatcatgtgt ctgatcacac tgaactatat
    44221 gaaatcgtga atcaaatact gtctgggatt tttgaattag gaccttatga gttcaaggaa
    44281 gaaaacggta atccaaaatt aaatgaagaa tggcaagaaa cgattgaaac acaaagaaac
    44341 aatccacact tatttcaata gtcttccaca gtatttcgta aaaatgttga tgaaaaagaa
    44401 acaccgtgaa tcagaggtcg tgaaaaacct gccctgcatc aagtaataag aacagccaaa
    44461 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagttttt
    44521 gaacaccgtt taaggaagag actgtgaagt caacgttctg acctgagtat tagagagaca
    44581 aatggcaaaa tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga
    44641 ttgaacaccg ttttatcgag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa
    44701 attttgtgaa aatggctttc cacatttttg cataaatttg acttaatacc gagaaaatgg
    44761 cttttgaaca ccgtttattc aaaaaaaggg actttgacac tacttttgtc aaagtcccat
    44821 cggtttttta tctattttta ttacaatcat ttcttccaga gtagtgacat ctggaatggc
    44881 tgaaatgtaa acagattatt tgcctgtatc aatgtcgaag agtaatataa aaagtgtaaa
    44941 cgctatcata ttgttcatca ttcaaatttt atagagagaa ggcattgaga tggaaaaaac
    45001 atatcaagca ggtacaaatg aaggaatagt agattttatc aatatggaag atttagaaat
    45061 cgcggcttca caagtgattc cagctggtgg ttatggttac atcagcagtg gtgctggcga
    45121 cttatttact tatcaagaaa atgaacgggc attcaatcat cgtttgatta ttcctcatgt
    45181 attaagagat gtagaattac ccgatacaac tacacatttt gacgaagaaa tgctgactgc
    45241 accaatcatc atggcaccag ttgctgctca tggattagct catgtgaagg cggagaaagc
    45301 ttctgcaaaa ggtgtagcag attttgggac gatctataca gcaagttctt acgcttcttg
    45361 tactttagaa gagataagag aagcaggcgg ggagaaagca ccgcaatggt ttcaatttta
    45421 tatgagcaaa gataatggaa tcaatttgga tattcttgaa gtggccaagc gtaatggagc
    45481 aaaagcaatc gtattgacag cggatgcaac agttggcgga aaccgtgaaa cagaccgcag
    45541 aaacgggttt accttcccgc tgccaatgcc gatcgttcag gcttatcaat caggagtagg
    45601 ccaaacgatg gatgctgtat ataaatcttc caaacaaaaa cttagtccaa aagatgtaga
    45661 atttatcgct gctcattctg atttgccagt ctacgtgaaa ggtgttcaat cagaagaaga
    45721 tgtctatcga tcattagaat ctggtgctgg tggaatttgg gtatctaacc atggtggccg
    45781 tcaattagat ggggggcctg ctgcgtttga ttcgttgcaa tatgtggcag aagcagtaga
    45841 taaacgtgta ccaatcgtgt ttgacagcgg tgtacgacgt ggacaacatg ttttcaaagc
    45901 aatcgcatca ggtgccgatc ttgttgctat tggcagaccc gttatttatg gattgtcttt
    45961 aggaggaagt acaggagtcc atcaagtgtt tgatttcttt aaaacagaat tggaaatggt
    46021 catgcaatta gctggaacac aaacagtaga agatatcaaa aaaataaaat tgcgtgagaa
    46081 tcgttttatt taagaaataa atgacgaaaa aaataggagt gcggcagatg atgccgtact
    46141 cctattttta tagttcaagg atgtcactaa tagaataatt tgaaacgaaa aaataaaatt
    46201 gaaatatttt ttcatactta ataaaaaagg aagaatattc atgtaaacga ttttattgtt
    46261 tttttctctt ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat
    46321 tttttgcata ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga
    46381 aaaagaacat gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac
    46441 cagcttttgc tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa
    46501 ataccgttga gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg
    46561 aaaatccagc aactggccag acaggttctt taacattgga tgtagttcct gaattgccat
    46621 ttggtacaca cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg
    46681 atacacctta tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag
    46741 ggtggaacgt gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg
    46801 ctgaattaga ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac
    46861 caactagtca aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag
    46921 cagcaaaaga tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac
    46981 tgaaagtgcc gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg
    47041 ctggcccagt agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg
    47101 tgaaggattt tttaccgcag ttgttgttga aaggatgtgg tggacaatga atcaaagtaa
    47161 acgactgttt gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag
    47221 ccaagcaaag gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat
    47281 agataaagca atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga
    47341 agtaattatc agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc
    47401 ggtaacgagt gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac
    47461 attgccgttt gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc
    47521 acatgcagaa acaactatac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg
    47581 tgtattagcg ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga
    47641 aggagcacaa atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca
    47701 agagacgata gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa
    47761 gccaactgtt cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt
    47821 tacgagcaag atcttttatg aaaatcagct ttatattgaa aatacgtcta atgaattttt
    47881 agttgcacca aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc
    47941 cggcgattac cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac
    48001 acaaaacttt acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc
    48061 agtacaagaa caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt
    48121 atggttgatt ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg
    48181 atgaggtgat ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt
    48241 tcttcagctg gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt
    48301 atgatctaag tccagttccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga
    48361 cacagctacc gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaag
    48421 aaaagatccg ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt
    48481 tgttatggag aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt
    48541 tttcgctcct tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg
    48601 gcagaagctg ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat
    48661 atcgtacctc caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact
    48721 ggggctaacc aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct
    48781 tggaaaggtg gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt
    48841 gatttggtga atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa
    48901 catgtatcaa gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt
    48961 gccggtgccc ccatcaaggt tactagcggt agtttattat cagcaggaag ccataccttg
    49021 cgttttggga tggggtcgtg ggatacaaat actctattgg cagatattat tgctattatt
    49081 acgcgaggcg aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc
    49141 gcgaagatga cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag
    49201 ggtaaattac aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg
    49261 aataatatta tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt
    49321 tatcctaaat taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca
    49381 gagataaata cgtggaatag ttacatctca acaagtgatt caacaggaaa atataataca
    49441 tttgctgata ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat
    49501 agtcggacat tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc
    49561 tttgatcgcg tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact
    49621 catactccag caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa
    49681 gatgcgcaag gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta
    49741 gacggaacgg ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct
    49801 tctgcatcgt accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc
    49861 cattacaggc tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt
    49921 ttccaaacga tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca
    49981 ttggcttcag ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg
    50041 tatccgtctt ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa
    50101 ataacagtgg ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga
    50161 caacctgaga caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg
    50221 acattcacgt taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt
    50281 cctgaaaata ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat
    50341 accggcattc aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc
    50401 attcatctta cttacaacta tcaattaaat actgtagata cttctgtaca atccgtcagc
    50461 atactgacaa aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt
    50521 cctaacccag ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca
    50581 ttagttaatg taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca
    50641 agttattatg aagcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc
    50701 acgtctcctt ggcaactgac agggacattg acttctttat ttaagaatga tcaaggtcaa
    50761 gaattaagcg gaacaaaatt atacttcaat cattctggat caaaacaatt gattcagcaa
    50821 ggtcaaaaca cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac
    50881 tttcccgata ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc
    50941 gcaacgtatc aaggcatggt cacatgggaa ctgacagctg ggccaaccag ctaagtgatt
    51001 agaatttgta tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa
    51061 acttctgctg ttaaaatcag aaatgcttgg aggagtggac ttggaacttt tcagtttgct
    51121 tgaaaaaaat gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga
    51181 gatttccgtt aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga
    51241 tctgttttat ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga
    51301 agggaatatt ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg
    51361 ctacttgcgc tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt
    51421 tgatcggcag aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt
    51481 gaaggaatta aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac
    51541 cggtccagaa agtcaaattt gttattttta tttccaatta ttttggtttg gacgtcctta
    51601 tcttgaaaac aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca
    51661 aaaagagttt agttttacta ttacagaaga aggacgattg aaatttgaaa tatggctttc
    51721 cattatgaag aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc
    51781 tgttggattg tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta
    51841 cttctttcaa tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat
    51901 gatcagtaac tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga
    51961 aaaatacaaa gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga
    52021 atatttctct ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat
    52081 gcaaaatcac gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg
    52141 attaaaacag cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt
    52201 ggtcagacaa acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt
    52261 tgaattattt gctcgctatg catctatttt gcggacgctg atcaagcagt tagaattacg
    52321 tctgcaaatt gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat
    52381 catccaggaa caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa
    52441 gcagtatgat atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa
    52501 agtttttgta ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt
    52561 aaaagaatgt tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc
    52621 attggtgctt cccatgcagg catcacagct gccctaaatc taagaaaact tcaaccgaaa
    52681 gcagaagttt tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga
    52741 atcaaccttt atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg
    52801 actcttcaag aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca
    52861 gacaaacatc agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat
    52921 aaattaatcg tagcaacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa
    52981 aatgtctaca cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa
    53041 gcaaaagaag tagtgatctt cggtgctggt tatattggat tggaattagc agatgctttg
    53101 agaaataaag gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat
    53161 ttcgataagg atatgatcaa cagttttcaa aatcagctgc aaactaagca aatcaatttt
    53221 tatccaaatg aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa
    53281 cttctatctc aagcaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat
    53341 acgacattgt taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa
    53401 tatctgcaga caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt
    53461 gacaaaaata aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga
    53521 gcagtagcgc taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca
    53581 gcagcagtca ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca
    53641 ttcttggaac aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgat
    53701 gaagaaaaca aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt
    53761 ggcggacagc tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc
    53821 gtgaaacaca aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa
    53881 cacacctctc gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca
    53941 aatagcaata gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga
    54001 tataataaat attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt
    54061 ttttgagcat aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt
    54121 atttcaggag gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac
    54181 gttatgcgtt agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg
    54241 ggttagctta tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt
    54301 cgattgtgct ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga
    54361 tagcagaaga agtcgaaggt gggaaagtgg cgtatcttgc ccagacacct ggagtcgtga
    54421 ctagtaaaga cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa
    54481 atcaattcat catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag
    54541 taggcgatat tagtttgaaa gaagaatatg gtctagccgt aggagataca gttaagttat
    54601 ctggtagtga caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct
    54661 caccagttct ctatacaacc atcaatgctt accaagagat ccgcttcgaa aaagaagata
    54721 caagtgaaaa tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg
    54781 aagatttaga acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc
    54841 aagtattgac ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg
    54901 ggattttcat ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac
    54961 aagggatcac aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat
    55021 ttgttggtat tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg
    55081 cggttccatt ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgctggtag
    55141 tagctgtgtt aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg
    55201 caattggata agaaggagga agagcatgaa agcaatagaa tttaaagaag tagaaaaaag
    55261 tttccaagat ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa
    55321 aggggaattt gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt
    55381 ggcaggcgga ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag
    55441 taatcaaaaa gaaagtgaac gcgttaaact acgttttaaa gaaattggct ttattttgca
    55501 agcttccaat ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat
    55561 caaacatgaa catcgtcaaa aagaagcaaa agaattattt gagcagcttg gcgtggataa
    55621 gttgatcaac aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc
    55681 tcgtgcattg tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga
    55741 ttcagaaaag gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa
    55801 agcaacgatc atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat
    55861 gatggaagat ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc
    55921 atcgatacaa caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt
    55981 ccaagggata tctcggcact tcaacaagag agatcgcaca aaaagttggg atcacccaac
    56041 ccaatctcta tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc
    56101 acttaaaaga tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc
    56161 aagtaacctt gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc
    56221 tgatgcttca cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc
    56281 tatggaagaa aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc
    56341 taagaaatgg gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt
    56401 acatcactcg gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt
    56461 tatatttgaa tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaaatagtta
    56521 ggtgaagaag caagatatga cgaagatcgt tatatcttgt tttttttata taggtagttt
    56581 ttcataaata caaatttgtt attctctcat gattatcagg gtatattttt atttattatg
    56641 tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa
    56701 ggaaactatt tattgggtag atgaatcacc acgaatagtc aaaaggagat ttcaggatat
    56761 gccaaaaatg aaaaaaatca ttttagcagt aatgttattt tctacggtta tatcttttac
    56821 agcccctttt ttgcgtactt aagctgattt taagttttga cccttagtct ggatctacga
    56881 ggtgagagtc aagaatgaaa aaaataattg gagcttttgt atttgcaatc attatcatta
    56941 ttatctgttt tcaaatagat gtttttggtc aaaaaaagat agttggacaa acggtcagca
    57001 gtacgaacag tacggttaca aagacaaagg aatcatttga tgatacagaa caagagacat
    57061 taatcaaaat cggaaaaaat gaccgtacac tatatacaga tgactcgcta actaccccac
    57121 ttgcaactat aaacggcgga gagctggcgg agtttctttc tgaaacaaaa aatgcttatc
    57181 agatcaagac aaatgatggc tatactggat atttagccct cgaagatgga accaaagtca
    57241 caaagaatat acaaacaaaa ccggaagagc tatctgatgc agtgattgta ttagatcctg
    57301 ggcatggcgg aaatgatact ggggcattaa gcaatgatga acaaacagaa gaaaaagata
    57361 taacattaag tacagccata aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt
    57421 atttgaccca tactacagat gaattagtac agcttggaga tatatgcgac tacagtgaag
    57481 aaaaaaaggc agatgtcttt atcagtttac acgcagactc tacggaatat gcgaatgaag
    57541 caactggaat cacgacatat tattactatg gacaagagga aacattagca cagacaattg
    57601 ctgactcctt cactgatctg cctctggatt caagaggaat ctccactggg aactatcaag
    57661 ttcttcgaga aaatcttcaa ccttctattt taatcgagat gggttatatg aataatgata
    57721 gtgatttaaa ggaattagta acagacagtt atcagcagca gattgctgca agtttgacac
    57781 aagcattgac cacttatttt cagtaataaa taagcccagc caaacaaaaa tagctcctca
    57841 tatttttcgt ttggccgggc ttattatcgc agatgcaagc ttttgaacac cgtttaagga
    57901 agaggttgtg agcctgccgt ttaactccgc gtcacaagga gcaatttcaa aaaacagctt
    57961 ctcatgtttg atgactattg tgacttgtga ccgaggagtt ggcatgagaa caccgtttat
    58021 tcggaacaag ggactgtgac atgacttttg tcacagttcc attatttttt tagcagcttc
    58081 aaagcttttt ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg
    58141 cctttctcca atagttttgc cgctacttgg tcagatttcg ttccagctcc tgatggaagg
    58201 gtgattccag cttcagctga tgctttgtgt aactcttcca aaaagctggc acgactgatg
    58261 atagaagcag cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt
    58321 tcttgtaatt gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac
    58381 tgatcgatca aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc
    58441 gcttgattgt gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg
    58501 tatttttctg gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca
    58561 agctggatga tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat
    58621 tctgcaagct gatcacgttt cacataagca gcacatactg ttagcggacc aaaatagctg
    58681 ccatttccga cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa
    58741 gaggctgttt ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct
    58801 attgctgcat tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc
    58861 ccgtcttttt ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga
    58921 tagtatgctt tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata
    58981 aaattctcct cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa
    59041 gagattgcga gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa
    59101 atatttgtgg agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca
    59161 aataggagga aatgattcgt atggcgcacg aaaaaacacg ctacaaagca gtgattgcca
    59221 accaaacata tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc
    59281 tgatcaatga acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag
    59341 cagcaatctt gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct
    59401 tggagctgga agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg
    59461 aaaatcgtgt gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa
    59521 atggtcaagc agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga
    59581 aacgaaaagg acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa
    59641 aaatttaaaa aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt
    59701 tattgctaat tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg
    59761 cgattggata tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc
    59821 atttggaatt atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg
    59881 atcaggtatt tgcttttcac ctagatgaag ctttttatgc aggaactgct tttctgatta
    59941 ttttgctaat tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt
    60001 atgtgccgat cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg
    60061 cttacgttac tatatttttg attttgcagt tacttgcctt tgttccatta gtgtggtcca
    60121 aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac
    60181 caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa
    60241 aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc
    60301 acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac
    60361 attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa
    60421 agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca
    60481 agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaact
    60541 tgaaaatatc cgtccccata tgaaacggat cgagatcgga gcagacttga atggagtgga
    60601 gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga
    60661 tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac
    60721 gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga
    60781 tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt
    60841 tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat
    60901 catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt
    60961 tggcggtgta gtccacgatc aaagtgcttc aggacagact ttatttattg aaccaaaaca
    61021 agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca
    61081 acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa
    61141 tgcttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt
    61201 aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca
    61261 tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca
    61321 agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg
    61381 tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat
    61441 cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct
    61501 atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa
    61561 aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct
    61621 agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca
    61681 ttatcctgaa ttgaaagttt atggatacaa tcgggcaaat acgattaatg ccagcatgga
    61741 gttcgatgtg gatacactaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag
    61801 caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa
    61861 gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg
    61921 ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga
    61981 acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat
    62041 ggaaaaagca aagaaaaaag caaacgaagt cgtgtcagag gcggaagaaa aagcagaaaa
    62101 aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca
    62161 tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa
    62221 aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt
    62281 ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt
    62341 agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca
    62401 aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc
    62461 gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata
    62521 tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac
    62581 aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt
    62641 tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag
    62701 cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata ctaatcatag
    62761 attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa
    62821 gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt
    62881 atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt
    62941 gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt
    63001 ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca
    63061 aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc
    63121 tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt
    63181 caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca
    63241 tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa
    63301 caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac
    63361 gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg
    63421 cagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt
    63481 tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc
    63541 taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga
    63601 aaaatatccc cgtttggtga ttactcgtaa agtactaaaa gacaaagcac tgtattttgg
    63661 accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc
    63721 tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca
    63781 atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga
    63841 agtgaaaaag tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat
    63901 ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg
    63961 tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga
    64021 tgtatttggt tatgcgattg acaaaggatg gatgtgtgtc caagtgtttt tcgtaagaca
    64081 aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga
    64141 tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt
    64201 cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct
    64261 gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgaataaga atgcctcagt
    64321 tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt
    64381 tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa
    64441 ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc
    64501 ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc
    64561 atcaatgaga gaagtcatct atcgcagata ttcacgagtg attcgcgaag ggttgccttt
    64621 acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt
    64681 agaaaatcaa ttaggaatcg atattcctat tgctggcatg gcaaaaaacg ataaacataa
    64741 aacaaatgag ttgctattcg gtatagattt ggctgtagta ccgcttgagc gaaattcacc
    64801 agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca
    64861 tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aaaggttagg
    64921 accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc
    64981 cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga
    65041 taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt
    65101 ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg
    65161 gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga
    65221 tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga
    65281 tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt
    65341 gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg
    65401 ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt
    65461 aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat
    65521 tgaaccaaaa atcgattttt ggatggaaaa atttcaggta aaagggaaaa agacggataa
    65581 agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca
    65641 tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga
    65701 attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag
    65761 ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa
    65821 aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt
    65881 cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat
    65941 ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat
    66001 ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt
    66061 ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca
    66121 aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt
    66181 ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca
    66241 ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa
    66301 cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg
    66361 aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc
    66421 tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg
    66481 gcttgcaatc gatgcttcga gcaagtcaat taggcatttc accagaagaa gtagctagct
    66541 tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga
    66601 cagttggaga agataactcg acagtccaat atgtcgtaag ttatgtagca acgattgtct
    66661 tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag
    66721 gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa
    66781 aactaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg
    66841 gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct
    66901 cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca
    66961 ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata
    67021 cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatatatg cttggattga
    67081 tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct
    67141 tatcttcata tattatgccg gttcgcttag caaatgagac agtcggtatg ggtggagcat
    67201 tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca
    67261 tgtacaaatc gaatgtactc gtgtatagcg aaggcggatt atggagttca ttgaaacaat
    67321 cgatttcaat tatgagaaat gaacgaaaaa aagcataaaa tatagaaaaa tatagaagga
    67381 agctggacat ttattgttcc cagcttcttt tttgtataga cctatttcta tttttttatt
    67441 atattggata tatatattca ttgaaaatag agcataacaa ctttataatg ttgagtatac
    67501 caatttttgg aggcgtttta atggaccgct atttagattt actttcagag aagttcccta
    67561 caacggaaga tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag
    67621 gaacagagtt atttttgagc gatatacatg gcgaatttcc tgcttttgat catattctgc
    67681 gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttctctgaa caaatgacgg
    67741 aaacggagat cagccaattc actattttta cggcttatcc agaatatgtg ttgacgactg
    67801 attggtacaa aaaacaagat aaatcccagc tgatccatcg tttgatcgat cttttacggt
    67861 ttacgacagt caaatacact cgatcaaaag ttcgaaaagg attgccgaaa gaatatagct
    67921 atatcattga ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa gactatgtaa
    67981 aaaaaatcat tgaacaactt gtcttgatga aggaagaaga aaaattttta acaaaacttg
    68041 cgcagacgat ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat atttttgatc
    68101 gaggaactca agcagccaaa gtaatggatc gaatcatgga tttttcctct gtagatatcc
    68161 agtgggggaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttat
    68221 tgacgctttt acggattgcc gcacgctaca actatttatt tgaattagag aaagaatatg
    68281 gattaaattt acgtccatta ttttcttttg cccatcaaac ctatcagaag aatccagtat
    68341 ttacccctaa aaacaggaaa gatctatctg aaagagagca agaagaatta gaaaagatcc
    68401 atcaagcact ttctatcctc caattcaaat cagaacatca gttgttgaat cgacgacctg
    68461 aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa tcaacgatcg
    68521 ttttagaagg tcagctctat gagttgaaag ggacttgttt tcaaacgatc cagccagatg
    68581 atcctaaaaa attcttggaa gaggaacaaa aagtgatcga tagtttgatg tattcattcc
    68641 agcactcgtt aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg ggaatgtatc
    68701 tcacgaacaa tcagcatgta ctgttccatg gctgtatacc tgtagatgaa aaggggcagc
    68761 tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctggga ttttttgaaa
    68821 aacagataac tgaaagcgca aaagatttga ctgcaaatga agatttggcg accgatttga
    68881 tttggtatgc gtggagcggg ccattttcgc ctttattcgg caagagtaaa atggcaactt
    68941 ttgagcgcta ttttctcaca gagcctcata cgcacgtcga aaaaagcaat ccctattatc
    69001 acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc agaattcgat gcaaaagaag
    69061 aaggttcagc aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga gagtcaccga
    69121 tcaaagcaaa cggaaaagca tttgttattg acggaggtct atccaaagct tatcaaaaaa
    69181 caaccgggat tgctggctac tcattactat gcaattctta cggtttccaa atcgtgacac
    69241 attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa acgaatgtaa
    69301 aaaaagtgat cgagcagcag ctcccacgca aacttaatcg gaatacgaca aaaggaatgg
    69361 aattacagca acagattact caactccaaa ggctgttgga ttatcgaaag gacgattgag
    69421 aaaaatgcta gccaacattg ctcaatagac taaaaagcga ctgcaacaaa aggcgtgtcg
    69481 cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt agaagctatt tttctgttta
    69541 tttctctaat actcaggtca gggcgctgat ttcacgacct cttccttaaa cggtgttctc
    69601 ctgccaactc ctcggtcaca agtcacaata tccaacaaac atgagaagct gttttttgaa
    69661 attgctcctt gtgactcaga gttgaacggc aggctcacga cctaatcaga aaaacgatta
    69721 aaattcattc acaaagaaat cgaataaacg ctgttcagat gaatctacac gatgagaaag
    69781 ttccggatgc cattggacac caaggatttt gtgcatatta tctttacttt ggactgcttc
    69841 aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat ctttgactgc
    69901 ctgatgatga taggaattca caaagtatga atcagggaga aggtcactta ataaactgtc
    69961 cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt gttcgtgttt
    70021 tacggaccat tttggatata atgaaagatc ttgataaagt gttccctcca acacgacatt
    70081 caatagttga agtcctcggc agactgcaaa aatcggtttt ctttgtttca atgcttccaa
    70141 gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt ttggatgagg
    70201 gtcttccatg taaagttgcg gactaacatc ctgaccgcct gcaagaatga gtttgtcgat
    70261 tttctcaatt agttgaggcg ctgaatctgg agacatgact ggcagaataa gaggtaatcc
    70321 ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta tagcttactt gatttccttg
    70381 gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg gtttcatttt
    70441 tattccccct aaagtagact aattgtaaca aggatttaaa aaaatttaaa ctatgtaaga
    70501 cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa ttttcgtgtg
    70561 aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa
    70621 taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt
    70681 attgaagggg cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag
    70741 aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa
    70801 caaatccgaa aagcagaaga agaaaatgag aagcgtgctc gaaccctacg gcagcagttg
    70861 aagacctatg cagaagaaaa agagcaagaa ttggagataa tccaaacgaa cagtcggcaa
    70921 cagctgaaac agttggtaga agaaaaagaa caattcgaaa agcaagaatt agaaaaagaa
    70981 aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga ccaatatcaa
    71041 aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac ttatggccgt
    71101 cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca
    71161 agcagtacaa ggaatccaaa atgttgaaat ccgggatctg tatcaaaata gaacaaacaa
    71221 ccagtgggta gaaaaatatt ttccagatac cacgattatt gataaagaag caaaattaag
    71281 cgctttaggc aatcggctga aagacatcca agaagctatc caattcatac aacaccacgg
    71341 cgatagccgt caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagacttgga
    71401 agcaaattat tccgaagaaa atttcatgaa taaattagaa gaagtccttg atttaaaaga
    71461 aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa gaagaggaag aatggcttct
    71521 tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga
    71581 gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactaa gtaagcttga
    71641 tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc
    71701 tttaaaaaaa tcagttgctg ctatagaaga aattgctggg aaatacgggt tcaataaaga
    71761 aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac agcaattatc
    71821 cattgttcac gagcagcaaa aaaagataac gacagcgtta ggtcagtgca gcggctatat
    71881 ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacggg aaaaagtaaa
    71941 agaacggttt atccatgcca cttatctgat cgttttgcaa ggttgggtag atgcagaaga
    72001 aaaacagaat ttgatgcatg tgttaaacga aactgtatca gaagaagata tatacgttag
    72061 tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca
    72121 tccgcttatc gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga
    72181 agtagatcca acgccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc
    72241 agatgttggt tacggattgc tgatgctcat tgggacaata ctagctcaaa aactatttgt
    72301 tttaccccgc gggatgaaac gtttcgtaaa attctttgag attctctcgg taccatcgat
    72361 catctgggga ttgatctaca gttcattttt cggacaggca ttaccaaaag aactatttgg
    72421 tatccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt
    72481 atccgtgatt ttcggtctga tacagatctt ggttggatta ttcgtggcag caaaggaaaa
    72541 tatcaggagg aaagattact tgagtgctgt cagtgatggt tttgcctggc aagggatcct
    72601 gattgggatc gttattgcat tgattggtgt catgttgata aaaaatacag catttgttta
    72661 tcttggcgcg gggctatcga tcattgctgc tttatgtatc ttaatcgtgc cgattatcca
    72721 atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat aatctttacg gattgactgg
    72781 ttatatcggt gatttagtca gttatacacg tttgatggca ctaggtattt caggcggaag
    72841 tattggcgcg gcattcaata tgctagttgc tttcatgccg ccagccgctc gattcagtgt
    72901 agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac tgttaagtgc
    72961 ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt atacaggagg
    73021 cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca atcataagaa
    73081 aaaaaacaat aaataattgg aggattttta tacaatgatg gattacttaa ttagtcaaaa
    73141 tggtggaatg gtatttgcag tattagcgat ggcaacagcg acgatttttt cagggattgg
    73201 ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa caagtcaacc
    73261 cgaaaaattc ggacaggctt tgattctgca attgcttcca ggtacgcaag ggttgtatgg
    73321 attcgttatc gcctttttga tttttatcaa cttaggaaat gatatgtcag ttgtacaagg
    73381 attgaacttt ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt caggtatcgc
    73441 ccaagggaaa gttgcagcag caggtatcca aattttagcg aaaaaacctg aacatgctac
    73501 aaaaggaatc atttttgctg cgatggttga aacatatgcg attttaggtt tcgttatttc
    73561 cttcctatta gtattgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa
    73621 aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt agagcaagaa
    73681 gagcgaatga agatcgatca aaactttgaa caaaaacgta cgcaaatcga aacagaacat
    73741 caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca gcttaggaat
    73801 cgccaacaag tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttacgacga
    73861 ttgtttgcag atgcaatagc tgaaatggag aattgggatg aatctgaaca aatccagttt
    73921 atcaaaaatt cattgtattc attacctttg acaggcaaag taacattcat tgctggagaa
    73981 aaatcagcag cttatctttc tcagacattg ctggatgaat ggaacaatga acttcccttt
    74041 atgatggttt taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa
    74101 ggagttcagt ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt
    74161 tttgagattg ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac
    74221 taaatccctt gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga
    74281 tgattcaaac aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc
    74341 catatattta tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc
    74401 gtctatttcg ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga
    74461 tgcgctatac tttccataat ctgaaggttt tgacaaaagc cgagatgaca aatcaaaatc
    74521 tggaccattt gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc
    74581 atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga cagtatacga gaagtacgtg
    74641 attattttga agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt
    74701 tgacagagca gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca
    74761 ttgcttttat tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa
    74821 catatgggtt catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt
    74881 tgaactttgt agataaagat atggaagatt acacgaattt tctattagag acagattatg
    74941 ctgattttct gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag cttgaacagt
    75001 tgaaagatga ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac
    75061 tgccactact agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg
    75121 ttatcggcaa aagaagccat ttcgaaccag aaacaatcag aaagcgggtg agacaagtct
    75181 atgacgcata agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt
    75241 ggctttgatg ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg
    75301 gcaaagaatg agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa
    75361 acgatcgaac gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa
    75421 ggatcattag ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa
    75481 aatatcttat agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc
    75541 tccggacctc ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta
    75601 gtgggagatc taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt
    75661 caagtatatg aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa
    75721 gctttatctg ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg
    75781 ccattagata cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg
    75841 cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg
    75901 gtgagtgctg gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc
    75961 atggtgccaa atgggataaa aggaacgatc aaaacaatcg aaaaaggttc attcactatt
    76021 gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa
    76081 aaatggccag taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg
    76141 atcactggac agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct
    76201 gtacctggtc cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc
    76261 gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt
    76321 ttaaatgaat tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg
    76381 gtattgattg ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg
    76441 ggaatcacaa ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac
    76501 tccacttcac gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct
    76561 ggtgatgaag gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct
    76621 ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct
    76681 gtatccccgt ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta
    76741 aaagtattct ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat
    76801 tggatacaaa gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt
    76861 caagctgact gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag
    76921 ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca
    76981 ttagaagtgg caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc
    77041 gatacattca cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt
    77101 gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc
    77161 gtatcgattc gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa
    77221 ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc
    77281 atgagcaatg attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt
    77341 tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga
    77401 gattcgtcgt ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga
    77461 aggaacaagt ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga
    77521 actaggtgta tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga
    77581 taacggacca gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc
    77641 agtagctcgg gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt
    77701 gaatacatta gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa
    77761 agaattagct gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc
    77821 cgtagtcttt gctgctattg gaatcacttt tgaagaagct gaatttttca tggaagattt
    77881 tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc
    77941 aatcgaacgt atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga
    78001 aaagggaatg catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg
    78061 agagatttct gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta
    78121 cacgaactta gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt
    78181 tactcaaatt ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt
    78241 gactggttat attacagaag gacagatcat cttgactaga gagctttaca agagtgggat
    78301 tcagccacca attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc
    78361 cggaaagaca agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca
    78421 aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa tctgctttat ctgatgtaga
    78481 caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta
    78541 cacgaaccgt tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc
    78601 aagaaccgaa ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa
    78661 gtgagttctg atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa
    78721 aaaacagttg acaactgcca caagagggca taaattatta aaagataagc aagatgaact
    78781 gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga
    78841 gtctatgaca gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggaaccttt
    78901 catcgaagaa ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa
    78961 tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac
    79021 acctttggaa tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt
    79081 tactaccctg cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat
    79141 ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc
    79201 tcaattagaa gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga
    79261 agtaacaagg ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatctttttt
    79321 taaaagagga aaacgatgat taaacaaagc aaacaaataa agaaacgttt gccggctgtt
    79381 cagcttctcg cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta
    79441 ccatttttta gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact
    79501 tctgctgtct gtgtaacggg attgacaaca ttgaatacag cagagcattg gaacagtgct
    79561 ggtcaatttt tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg
    79621 attctatgct ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa
    79681 gaagcgctga atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctaaaa
    79741 ttggcctttg ttatccaatt gattggtacg gttgctttat cttttgtatt tgtgcctgaa
    79801 tttggctggt tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat
    79861 gcagggtttg atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc
    79921 atggtggtat ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta
    79981 ctggaatatc atcagaaaaa gaagatgacg attcattcaa aaatcgcatt aaccgttaca
    80041 ggtacccttt tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggcaacg
    80101 ctagtggaag ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct
    80161 agaacggctg gatattattc agttgattac ctccatatga gccatgcagg tctgatttta
    80221 acgatgtttt tgatgtacat cggcggaaca tctgggtcga cggccggcgg attgaaaaca
    80281 actacgttag gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg tacgagagcg
    80341 gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt
    80401 gttacgttga cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct
    80461 aaaacttctg gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt
    80521 cttacgatgg gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt
    80581 atgtacattg gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa
    80641 agaacacaat caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt
    80701 ctgacagaaa cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg
    80761 gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg ttgaacctcg
    80821 ggtagtgcct cttttttatt gaaaggaatc aataagattt gattccttta tttatcaata
    80881 ggttataatt catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt
    80941 tcaaggttat tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta
    81001 aagcgtctgg aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta
    81061 gggtcaatct caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt
    81121 tctactttga aaagagaatc aaaaagctga ttggctcttt ttgtaagact taatttagtt
    81181 tctaagatcc tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat
    81241 ccagaagttt tcacatttat attaaaaatt gcttgttcta tcttgctttg gacatgtact
    81301 ggaactacaa gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag
    81361 tagattgttt ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg ttgatagtaa
    81421 gttgttaaaa atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact
    81481 tcggctaatt gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa
    81541 ggcataagaa agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt
    81601 cgcattcctt gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat
    81661 tcaatgaaat tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt
    81721 gcagaaagtg tgatagccaa tccattttga acaccggaaa caatagatag agattctaag
    81781 gacggaatga ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg
    81841 cctttgggat cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga
    81901 ataaacggga caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg
    81961 atatgttcgg ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata
    82021 aatgtgccac ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc
    82081 ttgccaacgg tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac
    82141 tgagtgtttt tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat
    82201 cgataataag gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca
    82261 taagaattaa taggcatttt ctccactcct taaaattgta taccatacaa ttttaccatt
    82321 gattgtattg attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt
    82381 aattttgagg aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa
    82441 tgtttttttg attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta
    82501 cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga
    82561 tattttggca caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct
    82621 catttaggag agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac
    82681 gcaaatgcag tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta
    82741 gcaaatccgc atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat
    82801 ttgactgcga tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa
    82861 gaaagacgaa atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata
    82921 aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct
    82981 agtaaagagg catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg
    83041 ggcacattta ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc
    83101 tatgtagatg attactcagc tgccttaaca gaaagcgggg acctgcttat agccatggca
    83161 gaaggagatt tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa
    83221 aaaacatttt ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt
    83281 gaagatcttt gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga
    83341 acttagatta ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag
    83401 cggtttatgt gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa
    83461 acaatttatc tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt
    83521 ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt
    83581 tatttaacta ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc
    83641 tatttgtgac aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa
    83701 aagtgccgat attgtctgaa tatgaagaag cgattggggc taagattaaa gaagcctact
    83761 tagcacgtga tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag
    83821 attttacagc tatcaaaaat tttgaattag gaataggagt catcgtgaca gcagaaagtg
    83881 tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt aacaatcaat gaagatccag
    83941 tatgtggatc agcacattct aatcttattc cttattgggc agaaagaatg aataaaacaa
    84001 aattgtcagc ttatcaaatg tcatcaaggg gcggggatct agaatgtgaa ttaaaagaag
    84061 atcgtgtgat aataggagga aaagcagttt tgttttctaa aggtacctcg tttatttgat
    84121 ttctttcaaa tgaattaaaa ataaattggt ggagagtggc aaccttatag ctacaatggt
    84181 tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag tttgcgtctc attttaatta
    84241 ttggtgtttg ttttttattc aatcaagagt aaaatgcccc atactaacta tcctcggatg
    84301 acttcgattt tataaccatc agggtcaatg acaaagtagt aagaaggcgc agtgccaggt
    84361 aatcctttta agtcagtaat ctcaagaccg gcagctttat gttgctcgtg taatttttct
    84421 agatcatcag ctgctatagc gatatgacca tagccgtctc ctagatcata cgcaccatga
    84481 tcatagttgt aagtcaactc taattcatag tcatccccag gaagtgtcaa ataaacaagt
    84541 gtaaatttgt tttctggaaa atcacggcgg cggctttctt caaaaccaaa tgccttttga
    84601 taaaattcga ttgatgcttc aagatctttc actcgaacac atgtgtgagc cattttcata
    84661 gattcatctc tccttttcct tttactcaat cataacaaaa agaagtaagg aaatagttga
    84721 tttgtgcttg ttcatgagaa atttctcatg taaaatggat agaaaataaa actgttaagg
    84781 tggaaactat tcatggataa accaattttt ggtgaaaaaa agcaatttgc tgattatcaa
    84841 acccgttatg cagcatatat tgtcatagaa agagatcagc agattgcagt aatcgaggct
    84901 ccaaatggtg ctttttttct tcctggcggg gaaattgaag gagaagagac gaaagaagaa
    84961 gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg cagaaatttc ttcttatctg
    85021 ggagaagctg atgagtattt ttactcgaat tatcgtgcaa cttattatta taatccagga
    85081 tatttctttg ttgctgattc ttggaagcgt ataggagaac caactgaaaa gaccaataaa
    85141 atttggtggg tgagccctca agaagcgctt gtgaaattga aacgtggtag ccaccgatgg
    85201 gcagtcgaga gatggatcga aaaaaataaa aactgaaaga aagcgagtag atcagctttc
    85261 tctcagtttt tttatttatt acagtacata ttttttgatt gcttcagcta caccgtgctc
    85321 gtcgtttgat gaagtgatga catttgctgc ttctttgaca agagaaacgg catttcccat
    85381 tgctactcct agaccagcat attcaatcat ggataagtcg ttctcgttat ctccgatagc
    85441 catgatttca tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt
    85501 acttgcttct ttatttagta cttcgaagta atagggcgca cttttgaccg ttgtgtattt
    85561 atcacggaaa gtttgcggta aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat
    85621 catcatcatt ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc gatattttag
    85681 cggcatcttg actaaacttg cttcgtggat cgtataagcg ctgatatctc gattaggcgt
    85741 gtagatcgct ttgtcatcga tcgaatggag atgactgccg acacggcgtg ccatcacttc
    85801 gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt
    85861 ttgttgtacg agtgccccgt tataagtaat aacgtaatca ttatcttgaa aaaggttcaa
    85921 ctcgtttaac tgttcctgga cacctggtaa gggacggcca gtgcataaaa cgatcgaaac
    85981 accttttgca acagcagcgg caatcgcttc ttttacttct tttgttactt ctcttttctc
    86041 atttaataaa gtaccatcga tgtcgatagc gactagtttg atagacataa tttcctccaa
    86101 tatattttaa tttatttcaa tcaatgcgcc attacggata tggctggcaa attcttgata
    86161 agtttcgtca aataaatcat aatgatcacg caaggacgga tcaaccattt cttttggaaa
    86221 gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca gcaacaagct gactaacctg
    86281 tgataattct atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc
    86341 ttgttctggg cgataaaaat cataaggtag atcgtagcta gaattcacag cagtgtaata
    86401 aacaggatta tatccgacct tttctactaa caaagtgagt tcttggacta attttgaatc
    86461 ccggttgcca gaaaaagtag cagatttcag tggtttacga ttcaaaaaac gtttagctaa
    86521 atcatttaaa ataggatcag cagattcaga ccattgagta aagtaagtgc ccaagacccc
    86581 atcatcaagt ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa
    86641 ttgagaatgg agttcaaaac tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag
    86701 cagatgctcc aaaattactt ccataccacg ggaaacagga tggaagtaca cttgcacata
    86761 catttgatat cggctaacga tataatcttc cactgcatgc atgccactca tggaaaaagc
    86821 gatcccgcct tcataaggac gaatcacacg taatattcgg gtaagatcaa atgttccata
    86881 ttctgttcca gtgaaataag catcccgaag caaatagtcc attcgatctg catcgatctg
    86941 actagaaatc atttggacaa cttgtgggtt ggggtaagtt ttttggatca cactagctac
    87001 cttttcagga aaaccttctt ctactcggtt aagaatctga tacacttctg tttctggaga
    87061 agtgatgatc tctacagtga tggcttcatg attcgtatgg aagatatgtt caaatgtatg
    87121 agagtaagga ccatgaccga catcatgcaa cagtgccgca cataaagtca ccagccgttc
    87181 ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa aaatttcgag aaaagatatc
    87241 gcagattcgt cttgaaatct cataaacgcc taatgaatga gtaaaacggc tatgttctgc
    87301 tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc
    87361 tttcgaattg atcaaatcta agattacttg atgctggaca tggatatagt tatggactgg
    87421 atcacgaaaa actttttcca taggcagcat ttgatattta taaggggttg tcataatgta
    87481 gctcctttat gttattacgt tgttccattc tctggatttg gttgttccat tgctccagct
    87541 ggatttcttt ttttagccag tcggtagaat cctgtaaatc acttgcagaa tacagctttt
    87601 caaaagcttt tgcaaatgct tctttgactt cttttacaaa aaggggttgg tcaagcagtt
    87661 cttcaagtgt tgccattgta gcaggatcca ctgcaggata gccatttgta ccaaagtctt
    87721 cttttaaagc agattgataa aatcgcttca tgacttgccc gcgtttttcc tgattaccgt
    87781 tgacacttaa gtacatcata acagaaatcc catttttgat ccgacgctga gcgataccgg
    87841 caaatttttt gccatggatg cttaaatcat aagttccagg acaataagaa tgggtgattt
    87901 cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt caataggagc atttgctcgt
    87961 aagcgtcatc tatggttatt ttttttgtgt cactttgggg gaaaatcaaa gaaatattca
    88021 aaattccttc atctgatacg atccctaatc cacctgaatt tctgatcact ggagcgtaac
    88081 ctgcatcttt cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca tccttcatac
    88141 ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg tgttctttag
    88201 aatattccgt taatatatct gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt
    88261 gctgatctag tcgaatcgca gggttcattc aaccggctcc tttgtataaa ttcatatttt
    88321 ctacaatatt atactctaaa atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat
    88381 ggacaagttg gttgacaaaa caaaagaaac taggaacaat gtagctatga gaagtaaagg
    88441 agaatgaata atggaattat cacaaggaac accgatttca atcaaattgc gtacaaaagt
    88501 aaaacagcaa ggggaagtgc aagattttta ttttgatttg aaagggcaaa tggtgacaat
    88561 tggcgatacg ctgtacatac gttataaaga gattcaggag gagacgggcg atgagattcc
    88621 tgtaacgatc aaattagttc cagatggtca tgtacagctg atccgtgcag gagaaatgcg
    88681 gatgcgtttg aaatttggct ataaagaacg gttagaaaca tcctatcgaa caccatacgg
    88741 tatgatccag attgctactt ttacgaaaga actgcatgtt agtttgaaag accgtcccac
    88801 aggagggaag gttcgaatcg attatgatct gttcatggga ccggaaaaaa tcggcgaata
    88861 ttatttgacc ttggatttta cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg
    88921 attttgtatg ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt
    88981 tgaaggtgca aacaaaagcg aattatcaat gatcgaagta gcacacgcga tcttagaaca
    89041 acgtggcgat gtcatggatt tttctgattt agctaaccag atccaaaact atcttgggaa
    89101 atcagacagt gaaattcgtg attcactcgc acaattttat actgacttga atattgacgg
    89161 cagctttatc tcattagggg ataatcgttg gggcttgcgt tcatggtatg ctatcgattc
    89221 gatcgatgaa gaagtaaacc atggaatgga cgaagaggat gaagatactc cacgtcgccg
    89281 taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg attacaatga
    89341 cgatgaccca gaagatacag atctgacaga agaagatgac gatgatctgt ttgatgacga
    89401 tgatgatgaa gatgaagaaa tcgctgctta taattctgac ttgcaagaaa tcggagcaga
    89461 tgacccgaca gatgatgaag aagacttgcc aggaaatatc gaagaagatt taagtatcat
    89521 agacgaagac gatgatgatg aagattatga agaggaagaa ttttctgact ctgaaaaaga
    89581 ataaacatta ttttcttacg agaggttgtg acagagacga tcagcctatc agaataagga
    89641 ggtgtgacac gattt
//
LOCUS       DAZUKR010000002        80727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000002 DAZUKR010000000
VERSION     DAZUKR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.002"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1651
                     /locus_tag="ACG89C_000091"
     CDS             20..1651
                     /locus_tag="ACG89C_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS4898683.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1713..2354)
                     /locus_tag="ACG89C_000092"
     CDS             complement(1713..2354)
                     /locus_tag="ACG89C_000092"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4898684.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2540..2616
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2636..2944
                     /gene="rplU"
                     /locus_tag="ACG89C_000093"
     CDS             2636..2944
                     /gene="rplU"
                     /locus_tag="ACG89C_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4898685.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2971..3315
                     /locus_tag="ACG89C_000094"
     CDS             2971..3315
                     /locus_tag="ACG89C_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS4898686.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3328..3621
                     /gene="rpmA"
                     /locus_tag="ACG89C_000095"
     CDS             3328..3621
                     /gene="rpmA"
                     /locus_tag="ACG89C_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4898687.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4055..6862
                     /locus_tag="ACG89C_000096"
     CDS             4055..6862
                     /locus_tag="ACG89C_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4898688.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7053..7544
                     /locus_tag="ACG89C_000097"
     CDS             7053..7544
                     /locus_tag="ACG89C_000097"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4898689.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7647..8636
                     /locus_tag="ACG89C_000098"
     CDS             7647..8636
                     /locus_tag="ACG89C_000098"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4898690.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8669..9472
                     /locus_tag="ACG89C_000099"
     CDS             8669..9472
                     /locus_tag="ACG89C_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4898691.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9493..10407
                     /locus_tag="ACG89C_000100"
     CDS             9493..10407
                     /locus_tag="ACG89C_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4898692.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10490..10864
                     /locus_tag="ACG89C_000101"
     CDS             10490..10864
                     /locus_tag="ACG89C_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS4898693.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11003..11248
                     /locus_tag="ACG89C_000102"
     CDS             11003..11248
                     /locus_tag="ACG89C_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4898694.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11420..12484
                     /locus_tag="ACG89C_000103"
     CDS             11420..12484
                     /locus_tag="ACG89C_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS4898695.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12730..13155
                     /locus_tag="ACG89C_000104"
     CDS             12730..13155
                     /locus_tag="ACG89C_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4898696.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13148..13597
                     /gene="nusB"
                     /locus_tag="ACG89C_000105"
     CDS             13148..13597
                     /gene="nusB"
                     /locus_tag="ACG89C_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4898697.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13694..14548
                     /locus_tag="ACG89C_000106"
     CDS             13694..14548
                     /locus_tag="ACG89C_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS4898698.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14549..15886
                     /gene="xseA"
                     /locus_tag="ACG89C_000107"
     CDS             14549..15886
                     /gene="xseA"
                     /locus_tag="ACG89C_000107"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4898699.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15891..16115
                     /locus_tag="ACG89C_000108"
     CDS             15891..16115
                     /locus_tag="ACG89C_000108"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4898700.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16117..17007
                     /locus_tag="ACG89C_000109"
     CDS             16117..17007
                     /locus_tag="ACG89C_000109"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4898701.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17016..17834
                     /locus_tag="ACG89C_000110"
     CDS             17016..17834
                     /locus_tag="ACG89C_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS4898702.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17941..18390
                     /locus_tag="ACG89C_000111"
     CDS             17941..18390
                     /locus_tag="ACG89C_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4898703.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18408..20090
                     /gene="recN"
                     /locus_tag="ACG89C_000112"
     CDS             18408..20090
                     /gene="recN"
                     /locus_tag="ACG89C_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4898704.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20145..20261)
                     /locus_tag="ACG89C_000113"
     CDS             complement(20145..20261)
                     /locus_tag="ACG89C_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS4898705.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20334..21290)
                     /locus_tag="ACG89C_000114"
     CDS             complement(20334..21290)
                     /locus_tag="ACG89C_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4898706.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21461..21829
                     /locus_tag="ACG89C_000115"
     CDS             21461..21829
                     /locus_tag="ACG89C_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS4898707.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            21999..22430
                     /gene="mraZ"
                     /locus_tag="ACG89C_000116"
     CDS             21999..22430
                     /gene="mraZ"
                     /locus_tag="ACG89C_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4898708.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22451..23410
                     /gene="rsmH"
                     /locus_tag="ACG89C_000117"
     CDS             22451..23410
                     /gene="rsmH"
                     /locus_tag="ACG89C_000117"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4898709.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23415..23816
                     /gene="ftsL"
                     /locus_tag="ACG89C_000118"
     CDS             23415..23816
                     /gene="ftsL"
                     /locus_tag="ACG89C_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4898710.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23817..26009
                     /locus_tag="ACG89C_000119"
     CDS             23817..26009
                     /locus_tag="ACG89C_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4898711.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26035..26997
                     /gene="mraY"
                     /locus_tag="ACG89C_000120"
     CDS             26035..26997
                     /gene="mraY"
                     /locus_tag="ACG89C_000120"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4898712.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27011..28372
                     /gene="murD"
                     /locus_tag="ACG89C_000121"
     CDS             27011..28372
                     /gene="murD"
                     /locus_tag="ACG89C_000121"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4898713.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28398..29486
                     /gene="murG"
                     /locus_tag="ACG89C_000122"
     CDS             28398..29486
                     /gene="murG"
                     /locus_tag="ACG89C_000122"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4898714.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29501..30721
                     /locus_tag="ACG89C_000123"
     CDS             29501..30721
                     /locus_tag="ACG89C_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS4898715.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30877..32202
                     /gene="ftsA"
                     /locus_tag="ACG89C_000124"
     CDS             30877..32202
                     /gene="ftsA"
                     /locus_tag="ACG89C_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4898716.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32225..33466
                     /gene="ftsZ"
                     /locus_tag="ACG89C_000125"
     CDS             32225..33466
                     /gene="ftsZ"
                     /locus_tag="ACG89C_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4898717.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33483..34160
                     /locus_tag="ACG89C_000126"
     CDS             33483..34160
                     /locus_tag="ACG89C_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS4898718.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34174..34773
                     /locus_tag="ACG89C_000127"
     CDS             34174..34773
                     /locus_tag="ACG89C_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS4898719.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34854..35075
                     /locus_tag="ACG89C_000128"
     CDS             34854..35075
                     /locus_tag="ACG89C_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS4898720.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35124..35906
                     /locus_tag="ACG89C_000129"
     CDS             35124..35906
                     /locus_tag="ACG89C_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4898721.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35927..36634
                     /locus_tag="ACG89C_000130"
     CDS             35927..36634
                     /locus_tag="ACG89C_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS4898722.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36896..39694
                     /gene="ileS"
                     /locus_tag="ACG89C_000131"
     CDS             36896..39694
                     /gene="ileS"
                     /locus_tag="ACG89C_000131"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4898723.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39743..41269)
                     /gene="zwf"
                     /locus_tag="ACG89C_000132"
     CDS             complement(39743..41269)
                     /gene="zwf"
                     /locus_tag="ACG89C_000132"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4898724.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41284..41931)
                     /locus_tag="ACG89C_000133"
     CDS             complement(41284..41931)
                     /locus_tag="ACG89C_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4898725.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42115..42444)
                     /locus_tag="ACG89C_000134"
     CDS             complement(42115..42444)
                     /locus_tag="ACG89C_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS4898726.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42621..43349
                     /gene="sfsA"
                     /locus_tag="ACG89C_000135"
     CDS             42621..43349
                     /gene="sfsA"
                     /locus_tag="ACG89C_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4898727.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43365..44378
                     /locus_tag="ACG89C_000136"
     CDS             43365..44378
                     /locus_tag="ACG89C_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4898728.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44378..45655
                     /locus_tag="ACG89C_000137"
     CDS             44378..45655
                     /locus_tag="ACG89C_000137"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4898729.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45718..48420
                     /locus_tag="ACG89C_000138"
     CDS             45718..48420
                     /locus_tag="ACG89C_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4898730.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48572..48889
                     /locus_tag="ACG89C_000139"
     CDS             48572..48889
                     /locus_tag="ACG89C_000139"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4898731.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48919..49239
                     /locus_tag="ACG89C_000140"
     CDS             48919..49239
                     /locus_tag="ACG89C_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4898732.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49347..50807
                     /locus_tag="ACG89C_000141"
     CDS             49347..50807
                     /locus_tag="ACG89C_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4898733.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50875..51096
                     /locus_tag="ACG89C_000142"
     CDS             50875..51096
                     /locus_tag="ACG89C_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898734.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51127..51309
                     /locus_tag="ACG89C_000143"
     CDS             51127..51309
                     /locus_tag="ACG89C_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS4898735.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51309..51722
                     /locus_tag="ACG89C_000144"
     CDS             51309..51722
                     /locus_tag="ACG89C_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS4898736.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51845..53026
                     /locus_tag="ACG89C_000145"
     CDS             51845..53026
                     /locus_tag="ACG89C_000145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4898737.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53097..53261)
                     /locus_tag="ACG89C_000146"
     CDS             complement(53097..53261)
                     /locus_tag="ACG89C_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898738.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53415..53488
                     /locus_tag="ACG89C_000147"
     tRNA            53415..53488
                     /locus_tag="ACG89C_000147"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53449..53451,aa:Arg,seq:cct)
     gene            complement(53534..53647)
                     /locus_tag="ACG89C_000148"
                     /pseudo
     CDS             complement(53534..53647)
                     /locus_tag="ACG89C_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(54030..55262)
                     /locus_tag="ACG89C_000149"
     CDS             complement(54030..55262)
                     /locus_tag="ACG89C_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4898739.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            55516..56085
                     /locus_tag="ACG89C_000150"
     CDS             55516..56085
                     /locus_tag="ACG89C_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4898740.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(56263..56703)
                     /locus_tag="ACG89C_000151"
     CDS             complement(56263..56703)
                     /locus_tag="ACG89C_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4898741.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            56861..57625
                     /gene="map"
                     /locus_tag="ACG89C_000152"
     CDS             56861..57625
                     /gene="map"
                     /locus_tag="ACG89C_000152"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4898742.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            57657..58580
                     /locus_tag="ACG89C_000153"
     CDS             57657..58580
                     /locus_tag="ACG89C_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4898743.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            58656..59795
                     /locus_tag="ACG89C_000154"
     CDS             58656..59795
                     /locus_tag="ACG89C_000154"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4898744.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            59788..60588
                     /locus_tag="ACG89C_000155"
     CDS             59788..60588
                     /locus_tag="ACG89C_000155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4898745.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            60588..61415
                     /locus_tag="ACG89C_000156"
     CDS             60588..61415
                     /locus_tag="ACG89C_000156"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4898746.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            61420..62127
                     /locus_tag="ACG89C_000157"
     CDS             61420..62127
                     /locus_tag="ACG89C_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4898747.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            62227..63093
                     /gene="rfbA"
                     /locus_tag="ACG89C_000158"
     CDS             62227..63093
                     /gene="rfbA"
                     /locus_tag="ACG89C_000158"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4898748.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            63107..63679
                     /gene="rfbC"
                     /locus_tag="ACG89C_000159"
     CDS             63107..63679
                     /gene="rfbC"
                     /locus_tag="ACG89C_000159"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4898749.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            63701..64729
                     /gene="rfbB"
                     /locus_tag="ACG89C_000160"
     CDS             63701..64729
                     /gene="rfbB"
                     /locus_tag="ACG89C_000160"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4898750.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            64827..65678
                     /gene="rfbD"
                     /locus_tag="ACG89C_000161"
     CDS             64827..65678
                     /gene="rfbD"
                     /locus_tag="ACG89C_000161"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4898751.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            65713..67746
                     /locus_tag="ACG89C_000162"
     CDS             65713..67746
                     /locus_tag="ACG89C_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898752.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            67790..69070
                     /locus_tag="ACG89C_000163"
     CDS             67790..69070
                     /locus_tag="ACG89C_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4898753.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            69280..70086
                     /locus_tag="ACG89C_000164"
     CDS             69280..70086
                     /locus_tag="ACG89C_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898754.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            70098..71318
                     /locus_tag="ACG89C_000165"
     CDS             70098..71318
                     /locus_tag="ACG89C_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898755.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            71308..72894
                     /locus_tag="ACG89C_000166"
     CDS             71308..72894
                     /locus_tag="ACG89C_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4898756.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            72933..75071
                     /locus_tag="ACG89C_000167"
     CDS             72933..75071
                     /locus_tag="ACG89C_000167"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4898757.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(75439..76422)
                     /locus_tag="ACG89C_000168"
     CDS             complement(75439..76422)
                     /locus_tag="ACG89C_000168"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4898758.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            76800..78191
                     /locus_tag="ACG89C_000169"
     CDS             76800..78191
                     /locus_tag="ACG89C_000169"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4898759.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            78207..79247
                     /locus_tag="ACG89C_000170"
     CDS             78207..79247
                     /locus_tag="ACG89C_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4898760.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            79266..80351
                     /locus_tag="ACG89C_000171"
     CDS             79266..80351
                     /locus_tag="ACG89C_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4898761.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            80384..>80727
                     /locus_tag="ACG89C_000172"
     CDS             80384..>80727
                     /locus_tag="ACG89C_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4898762.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSS"
BASE COUNT        26394 a        13605 c        17104 g        23624 t
ORIGIN      
        1 tgaaaaagga gttagatagg tgttcaaaaa ttatgtttcc tcactaaaaa aagaattttc
       61 aggttatgat ctttcaagat ttcagaaaga tcttttggct gggatcacag tagctgctgt
      121 agcattgcct ttagccttgg cttttggggt atcaagcgga gctgatgcgg ccgcaggttt
      181 gattacggct atcattgccg gtatcgttat tggcggttta tcaggcggtt tttatcaaat
      241 atcaggtccg acaggtgcga tggcagctat cttgatgtct attgcggcaa caaagggaat
      301 gcaaggtgta ttgcttgcta cttttttagc gggtctattt ttacttgcag caggactttt
      361 ccgattagga acactgactt cttttatccc agcacccgta attacggggt ttacttcagg
      421 tattgctatc attattgcat taggtcagat cgataattta tttggtgttc actcggaagg
      481 tacaaacgtt attgaaaaac taagtagtta tcaaacgata ggatttccga taaacacagc
      541 ctctttgatc atgggagggc tagtcatttt agggatgttt atctatccaa aaaaatgggc
      601 gaaacgaatg cctagttcgc tgctggctat cattttggca acagcagtta tgctattgtt
      661 ccatttacca gtagcaactg tgggtaagat tccacaaaca ttgatcagta gtaatcgttt
      721 gaatatggga gattttagct tttctgcctt gcaacaagtg gcagttccag ctattagcat
      781 tgctttatta ggaatgattg aaagcttgct atgtggtgct tcggctggcc gaatggcaaa
      841 taagccctta gatagcaatc aagaattaat ggcgcaagga attggaaatc tgttgcttcc
      901 tttctttggc gggattcctg ctactgcggc catcgcccgt acaagcgtgg ccatcaaatc
      961 tggtgcacaa actagattag ctggtatgat acatgcagtt gtcttactgc tctccatgct
     1021 tgtttttgcg ccagtgatgt cacagattcc aatgccagca cttgcaggtg ttttgattgt
     1081 gacggcttgg cgtatgaatg aatgggaaac gatcaaggag ctatttacta ataaatattg
     1141 gtcagcagtt ctattgttta tccttacgat gatttgtaca gttatctttg atttaagtat
     1201 tgctatcgtg atcggggtga tcggcggctg tgtatttttt gttgtcaaaa gcgctgctat
     1261 cacgatttca ttagaaacga tcgattggca aagaatgggg ctgccagaaa cggaaaagct
     1321 gaataactgg acagtagtgt atattagtgg tccactgttt tttatgtctt cagaaaaact
     1381 caaaaataaa ttggaagaac taaatcatca cgatggtgtc attttctcca tgcgaggcgt
     1441 accaagtatt gatttgacgg cacgtgcagt ttttgatgaa tttcaagaaa aagcagagca
     1501 aagaggacaa acgattatct atacttcctt gcagccagaa gtagaaaaac aactttctca
     1561 cttgtgggaa catacattca cgcatcaaca tcaaacagtt gctcatgcgt tgacagcttt
     1621 acatgcgaaa tatactgcaa ttagccatta aaacaatgaa aaagaacaga caagactgta
     1681 aactgaagat tttatggtct tgtctgttct ttttaatatt gctgtaaaag tgaagctagt
     1741 acttgccagt cttgatggat aacaggtaca agggagcgat tgtagaaaat agctgctgga
     1801 tgaaaaagtg gaagaattcg atagtttttt tctgaccatt cgtaggcatc cttttgttct
     1861 gttaattgta agacaggccc tgtatagagt tgaccatgat ttttggtaac cgtatattct
     1921 ttgccaagta aacgttgaag accaatgttt ccaagtgtcg caattaattt tggctggacc
     1981 ttcatcagtt cgtaatccaa tattggtgca tgcgcaaata cttcggaacg agtaggtgtc
     2041 cgatttggat aaacagtttc tgactgttgt gtccgtttat tgatccgatg agtcacgcga
     2101 tacgggcggc tccttacagc actggtgata tacacctctt ctcgtgttaa gccagttgaa
     2161 gaaagtgcct gcatcaattc ttttccagct tgaccgctaa aaggaacatg cgtatcaatc
     2221 tctgttttcc ctggggcttc tcccacgagc atcagtttag gatgaagagg tccttgccca
     2281 gcgataaatc cttctacagg acggccgttc attcttaaag aagcttcttg tatcagttct
     2341 tttgggtatt ccatttatgc tcctcctttt tcttttatta taagaataaa agaaacacaa
     2401 tgcgactatt ttatttggta gaactgtgag gatagaaaag cagaagtatt gcttgaattt
     2461 ttgttttact ttttgatttt aaatttagtt gactaacggt tgctgttctg gtaagctagt
     2521 actgttgtaa ttgtggactc cacagctaca accgctcaga acaagtaata agcacagcat
     2581 atgctgtcac ttcggatggc gagtctaagt atatcagaag gaggtgcttt atagcatgta
     2641 cgcaattatt aaaactggtg gtaaacaagt aaaagttgaa gtgggacaag caatctatgt
     2701 tgaaaaactt gacgttgaag caggagaaaa agttgttttt gacgaagtta tcttagtagg
     2761 cggagaatct acaaaagtag gcgctccaac tgttgcaggt gcaactgttg aaggtactgt
     2821 tgaaaaacac ggcaaacaaa agaaagtcgt tactttcaaa tacaaaccta aaaaacattc
     2881 acaccgtaaa caaggtcatc gtcaaccata tacaaaagtg gttatcgacg cgatcaacgc
     2941 ttaatagcgt ttgagaaaga aggcctatat atgatcaaag gaacatttaa gcgaaatgac
     3001 gcaggtcaga tcgtttcatt cactttgacc gggcatgctg atgctggtcc ttatggaagt
     3061 gatatcgttt gtgcgggtgt ttctgcgtta gcgatcagca ctgtcaacgg gattgcctca
     3121 ttagccggtt ttgagccgat tgttgaaatg aatgaagaag aaggcggtta tctttatacc
     3181 gaagtcactt caggaatgac acaagaacaa aacaatattg ctgagattct attagaaaat
     3241 cttttattag gattacaatc gatagaagct gaaaactcag aatatatcca aattaaaaca
     3301 attaatgata aataggaggt gcagaccatg ttgttatcta tgaatctaca attatttgcc
     3361 cacaaaaaag gtggcggttc tacatctaac ggccgtgact cagaatcaaa acgtctaggt
     3421 gctaaacgtg ctgatggaca aacagttaca ggtggatcaa ttctttaccg tcaacgcggt
     3481 acaaaaatct atccaggtgt aaacgtgggt atcggtggag acgacacttt atttgcaaaa
     3541 gttgatggcg tagtacgttt cgaacgtaaa ggccgtaaca aaaaacaagt gtctgtttat
     3601 ccagtagctc aagaagctta attgaatctt atgattaaag aggttgtgac aagacttttg
     3661 tcacagcctt ttttgtttat aaaaataaaa gttgtagtgg aggctacgaa gtttactttg
     3721 tctttttaca cttaatattt ttttgccaat ttggaatcag gatgttttga taactatcgt
     3781 tttcaattga tggaagctat attgtttgct atcagtgaaa taattatatg gtaaacagtg
     3841 ttctttttct tgtttccatt ataaaaatcg tatcgggatt tttcgttctt ttaaattgaa
     3901 catttctatc tatccgcgtg ttatcaacaa aacaaaatca ggtaaatagt tgacgaaaca
     3961 gaaataaata catagtatta tgttttgaag cctaagtaca atagtctata gaaaaggagc
     4021 gataccattg gcaaaacgca ttgaccgaat ttatatgtat gtcaaagaaa aaaccgctca
     4081 tttaacaaag gcagaatatg atcaaggagt aacaacacag gaaattgctg aagtgttagg
     4141 gattcagcga acaaatagca gtaaagatct gaatcagcta gtgcgcgaag gaaaactgat
     4201 aaagacagac ggacgaccag tacgttacat atatcaaaca ctcgttacgc acgtaaagcc
     4261 agttacaaaa catgtagtca gttacaaaga aacgccggtc tctgaagaaa aaaatacact
     4321 ccaaatagat actaaggata tctttgcaaa aatcattgga gctaacggca gcatgaaaaa
     4381 ttcagtggaa caggcaaaag ctgcgatttt gtatccgcca agaggattaa attgtttgat
     4441 tacaggacct accggatcag gaaagactta ttttgcacac gcgatgtatc attttgcaaa
     4501 agcgcatcat gtaatagatg aggaaacgga actgattgtt ttcaattgtg ctgattatgc
     4561 caataaccct gagcttttga tgagtcattt atttgggtac gcaaaaggtg cttttacagg
     4621 cgcagatgaa gaaaaaatgg ggattatcga tcaagcggat ggcggtatgc tgtttttgga
     4681 tgagattcac cggttgccgc ctgaaggtca agaaatgatc ttttacttta tggatcatgg
     4741 aacttacagt cgtcttgggg aaacaacgaa atcccatgaa gcaaatgtac ggatcgtagg
     4801 tgcgaccaca gaagacccag gttcttctct attagaaact tttgttcggc gtatcccaat
     4861 caatatcaaa cttccggctt ttgaaaaacg tcctgcaaat gagaaagtcg atttagtaaa
     4921 aatcatgatt gcccacgaag caaaccgcac gcagcgaaag atttctttga cagaagatgt
     4981 agcaaaagca ttgatcggca gtgtgaccta tggaaatata ggacaattga agtcaaatat
     5041 ccagttagta tgtgcacgag gatttctaaa tcatatgcaa tcacccgaaa tctcgatcac
     5101 aatcgatgat ttgacagaag ggattcgtag cggattgatt caattagcaa gcaaccgcag
     5161 tgcaatggca gagctttcta aaatattgga gccaaagata actgtagcac caaatgatac
     5221 gatcatgaaa atccaatcgg actcctatga gttaccttat aatctatatg atatcatcgg
     5281 agataaagca gctttactga agtcggatgg tttggatcaa gaagcaatca atcatttcat
     5341 ttctacagat atcaacatcc atctgaagtc tttctataaa gaccatggtt tttcttttaa
     5401 tgcagacagc aaattagcag aatttgtaga tccgaaaatc atagaagtga ccaatcagat
     5461 ttatgtaatg gtcaaaagat ctttaccata tgagtttcag caaaacttta tttatgctat
     5521 gagcttgcat atcagttctt ttttgaaaaa aatcaactta ggagaagaac ggcatacgaa
     5581 tgataacatc cgtgagatgg cttttgattt tccagatgaa tatgctgttg cgaaagaagt
     5641 gcgtagttat atcgaaacgt attttcaagt ggagattcca gaaagtgaag actattactt
     5701 gaccgttcta ctcgtctcgt tacgggcaaa tcaagcaagc ggcagaatcg gtgtagtcgt
     5761 tgcagctcac ggaaacagca cagcaagcag tatggtccaa gttgtccaac agttgttaga
     5821 tgcagagaac gtacgagcag tagacatgcc tttggatatg gatccgaaaa cagcattgga
     5881 ccgaattgaa cgcaatgtac aagaagtaga tgaaggaagc ggggtcatac tcttagtaga
     5941 tatggggtcc cttgcgtcgt ttaattcgca aattcaaaga gacactggaa ttccagtacg
     6001 tactgttgat atggtaacaa cttctctagt tttggaaact gtacgaaagg tctcagtatt
     6061 agggacagat ctagatatgt tgcatgaatc attgaaaaat tttcgaggtt acgcagaaat
     6121 atcttctgta gaatctgttc aggaagcaaa acctattcat aagaaaaaag ctgttttggc
     6181 tatctgcgca tctggcgaag gtacagcaca aagaatcaaa gaattgatcg aacgagcggt
     6241 aaacaagcgt caagaaacag aattagaagt tctagctttg tcgattgtgg aagtgaaaga
     6301 agaactgcca gttatccaaa aagaatatca gatcatagct actactggga ttactgattt
     6361 gaatatccaa gccccattta ttcctttaga acgttttatt gatcaaaata tcgaagtcat
     6421 tttggatcag ttgctgatgg aatcagaatt ggaggaaact gaattcatct ccctagatga
     6481 agagtcagct aaaaatacgt gtgttgagtt tatctctgat aacttcatct ttattaatgg
     6541 tagtaagtta atcgatccaa tgtggcaatt ttctacacaa atctctcaga cgacgggaat
     6601 cggcgatgaa gaatatggat tcaaaatcaa cctagtgatg cacacagcag gaatgatcga
     6661 acgtattatt cggaatgaac cattgactgt ggaagaaaac gaattgacaa atacaacgaa
     6721 tgatcctctt tatagccaat tggcagcctc agttgttctg ttggaagatc agataaaagt
     6781 aaaagtgccg atagaagaaa tgtattatct gctacgtcta gtccataatc agttagataa
     6841 aaaagaatac actgtgccat gaatacacaa aaacgataca cttttatagt gtatcgtttt
     6901 tgtgtattgg ttaagaatcc tttaaattca attagtgtat cgtaaaaaag ttggcacgct
     6961 ttttgcttat agtagtgtgt gctaggaggt ggttgttcac acagaaatga taggggtttc
     7021 tgtgaagtag tgaggaagaa ggagttttgc agatggatat tcgattggca cgtatagatg
     7081 atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa gcaaacaggt gttgaacgta
     7141 tcattgtgat cagcgatgta gtcgctcaag ataaattgag aaagtttcta ctaaaagaag
     7201 ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca gaaaatgatt caaatctatc
     7261 atagtggatt attatcacga gcaaaagtga tgcttttatt caccaatccc caagatgttg
     7321 aagcggttgt ccgctgcggt gtacagttga agtcgttgaa tatcggcggt atgagtttta
     7381 ctgaaggcaa atcaatgatt accaattttg tttcagtaaa tcaacaagat attgattcct
     7441 ttcgttactt gaacagtcaa gaagtggagt tagagattcg aaaagtacct gctgacagga
     7501 aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt gtagcaagaa tggttgttgg
     7561 aaaaggtcaa ataattctgt tgtaatggtt agcaattatt aatatttttg aatttgttac
     7621 aacaaaagta aataggaggt ataaatatgg tagggattat cctagcaagt catggacaat
     7681 ttgctgaagg aatcttacaa tccggttcaa tgatctttgg cgaacaagaa aacgtcaaag
     7741 ccgtgatttt gaaaccaagt gaaggtccag atgacttgag agccaaattg gaagaagcgg
     7801 ttgcttcttt tgataatcaa gacgaagtgt tattcttagt tgatttatgg ggcggaacac
     7861 cgttcaatca atcaaacaca ttattcgaag agcacaaaga taaatgggca atcgtcagcg
     7921 gtttgaattt accaatgttg attgaagcct acgcatcacg cttttcaatg gaatctgctc
     7981 atgaaattgc agcacacatc attgaaacag caaaagacgg tgtaaaagta aaaccggaag
     8041 aattagaacc ggcagaagca ccgaaagcag cagttgaaga cgcacaacct aaaggagcac
     8101 ttccagaagg aactgtggtc ggagacggaa aaatcaaata tgtattagca cgtgttgact
     8161 cacgtctgtt acatggacaa gttgcgacag cttggacaaa agcagtgcaa ccaaaccgta
     8221 ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg taagagacta atcgaacaag
     8281 ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag caaaatgatc gaagtagcaa
     8341 aagaccctcg ttttggaaat accaaagcat tgcttttatt tgaaaatcct gaagacgtat
     8401 tgacagctgt tgaaggtggc gtagacatca aagaattaaa tgttgggtca atggcgcatt
     8461 cggtcggtaa agtcgttgta agtaaagtat tgtctatggg acaagaagac gttgaagcgt
     8521 ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg taaagtacct aatgattcac
     8581 gcgataacat ggatgatatt ttgaaaaaag caaaagctga attagcaaaa gcgtaataaa
     8641 taatattaaa atttatagga ggcttatcat gtctattatt tcaataattt tagtcgtatt
     8701 agttgccttt ctagctggta tggaaggtat tcttgatgaa tttcaatttc atcaaccact
     8761 agtggcatgt acattgatcg gtttagttac cggtaacctc gaagctggta tcgtacttgg
     8821 cggtacgctt cagatgatcg ctttaggttg ggcaaatatc ggtgccgctg tagcaccaga
     8881 tgctgcatta gcatcagtag catcagcaat tattttagtt ttaggtggac aaggtgttaa
     8941 aggtgttcct tctgcgatcg cgattgccgt tcctttggct gttgcagggt tgttcttaac
     9001 aatgatcgta cgtacaatcg ctgttccaat cgttcatttg atggatgcgg ctgctgaaga
     9061 aggaaacatc cgtaaggttg aaatgtggca catcattgct gtatgtttac aaggggttcg
     9121 tatcgcggtc ccagcagcag cgttgctatt tatcccagca caaactgtgc aatcattcct
     9181 tgaatcaatg ccagcatggt taacagatgg tatggctatc ggtgggggaa tggtcgtagc
     9241 tgtaggttac gctttggtaa tcaacatgat ggctacaaaa gaagtatggc cattcttcgt
     9301 tattggtttc gttgtagctg caatttctca attaacattg attgctctag gtgctttagg
     9361 tgttgcatta gctcttattt acttgaacct ttctaaaatg ggtggttcat caaataatgg
     9421 aggcggaagc aactctggtg accctttagg cgacattttg aatgactatt aatcttgaag
     9481 gaggaaaata aaatggcaga agaaaaaatc aaattatcaa aaaaagatcg tttagctgtt
     9541 gcgtggcgct ctaccttcat tcaaggttca tggaactacg aacgtatgca aaacggtggc
     9601 tgggcatttt ctatgattcc agcaatcaaa aaattataca aaacaaaaga agaacgttct
     9661 gcagcattaa aacgtcactt ggaattcttt aatacacacc catacattgc ttctccaatt
     9721 ttaggggtta cattggctct ggaagaagaa cgtgcgaatg gcgcgcctgt tgatgatgta
     9781 gcgattcaag gggttaaagt aggtatgatg ggtcctttag ctggtgttgg tgacccagtg
     9841 ttctggttta cagtacgtcc aatgctaggt gcactaggtg cttcattagc tatgggagga
     9901 aatatccttg gaccaatcat cttcttctta ggttggaaca ttattcgttg ggcattcatg
     9961 tggtatacac aagaatttgg ctacaaagct ggttctaaaa ttactgatga cctttctggc
    10021 ggattgttac aagacgtaac aaaaggtgct tcaattttag ggatgttcgt attagctgcc
    10081 cttgtacaaa gatgggtatc aatcaaattc ttaccagttg tatcaactgt aaaattagat
    10141 aaaggtgctt acatcgaatg ggataaattg ccttcaggtg gtgaaggaat caagagtgca
    10201 tttgaacaag ttaataatgg cttagccctt tctcctgaaa aggtaacaac attacaagac
    10261 aacttagacc aattgattcc tggtttagct gcattaggat taacattgtt atgtatgtgg
    10321 ttgcttaaga aaaaggtttc tccaatcgtt atcatccttg gtttattcgt agtcggtgtt
    10381 gttggacacg tcatcggact tttataatcc ataaaaatat agctcgggcg ttgtcccgag
    10441 ctttttttat atagtatact gttcataaac aaatatctgg agggagaaaa tggttcaatc
    10501 aatcaataca aaagtagact tggtaattga tgctacttcg tttacgggtc tgacagatta
    10561 cgggcaaatc atgataggtg ataatggttt tgagttttac catgcgcgag atagacgcaa
    10621 atatatccaa attccatggg aagaagtgga ttatgtacta gcatcagtca tgctaaaggg
    10681 aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat ggtacataca cattttcttc
    10741 caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat gtagatgaag atagaatggt
    10801 acaatcattg agtttctttg atgtagtgaa acgtggtatt cgtagtatgt ttaaacgcaa
    10861 gtaaaaaata aagaaaactt ctaagaggag gttttcttta tttttttact ttgcagaaga
    10921 aaatatgaaa tagcaaaacc aattatttgt cccctttaaa tcccatatgc taaaattgat
    10981 tagcaaagaa ggaggttata ttatgagttg gttatggaca ttgattgttg gagccgtgat
    11041 cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg taacgaatat
    11101 cattgctgga ctagtcggtt caagtattgg acaagcgatt ttcggcgaat ggggaccaaa
    11161 agctgcaggt atggcactta ttccatctat tttaggcgca gtgattttgg tagcagttgt
    11221 ctcatttttc tttggaaaac gatcataagt ttttacaaaa tagtaaacta aaaggagttg
    11281 cagcgagact gtgactcctt tttggcttta tttatttaaa ataaaaagaa gttttccgga
    11341 aatttttatt taactgtcgt gtacgaagaa aagtttgcta taatggaaaa aagcgtgcaa
    11401 agaaagaggg agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa aaatgcaaga
    11461 agaaaatttg gattcatttt tggttaccag tccgtacaat cttcgttact taacgaactt
    11521 tacaggtacg acaggattag ctgttattac tttggaaaaa gcatttttta ttactgattt
    11581 tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt gaaattatta aaaatgtcgg
    11641 acctattttt gatgaggtag caaacttagt acgcaaagaa gaactgcact cattagcttt
    11701 tgaggaaaca acagtctcat ttttagaata ttcggtttta gaagaaatca tcgatgcgga
    11761 attagtacct gtttcacaga tgatcgaaga actcagagaa gtgaaagatg aagaagaaat
    11821 cgctattatc gaaaaagcat gtcatattgc ggatatggca tatgatcaca tcctgaaaat
    11881 gatccaaccg ggcatgactg agattgaagt cgcaaatcaa ctagactttt atatgcgttc
    11941 tcttggcgct tcaggtgttt cttttgatac gatcgttgct agcggattgc gttcggctat
    12001 gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt gacttgatca cattggactt
    12061 tggttgctat tacgaagggt acgtttccga tatgacacgt acattcgcta ttggtgatcc
    12121 tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat gctcaattag cagttattga
    12181 tgctgcaaaa ccaggaatga ctggtgtcca attagatgca gttgctagag attatatttc
    12241 aaaacatggt tacggggaag catttggaca ttctacagga catgggatcg gtttagagat
    12301 ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag gaaatgtgat
    12361 cactgatgaa ccagggatct acttaccagg aatcggtgga gtacgtattg aggatgactt
    12421 gttgatcaca gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat tgatcatctt
    12481 atagtttcat caatagaaga agtaagcgta aaaatcagtg ttcacagact ttgttataga
    12541 aaatcactcg atttaatgat acactataga agaactttta agctgtaaga gataataaag
    12601 ttgggtgttt tctccaattc atagcagttt gcagctgatc ggcggcagtt ttcttaccac
    12661 gtgatcacta tgccgcatca tagagaattc ggatgtatct cgtgcttagg caaagggagg
    12721 aataaataaa tggcagatga aaaaaatttg attttaaacg caaaccaaga tttaggagag
    12781 atcgtgatcg ctcctgaagt catcgaagta attatcggga ttgcagcttc taaagtagaa
    12841 ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt aggacgtgca
    12901 gctcacggaa aaggtgttta cttagcaaac gaggaagatg gcttgaaagt agatctttac
    12961 tgttatctag aatatggtgt ttcagttcct aaagtagcaa tggaaatgca ggaacgggtc
    13021 agccagcaag tccttttcat gacggatatc gatttatctg aagtcaatat ccatgtagta
    13081 gctgttgttc ctgaaaaatt agaacaacca acaatcgaag aactttttga agacgaagag
    13141 gaagagaatg aataaagaat tatcaagaca cgagattcgg gaaatggcat tgcaggcttt
    13201 atttccatta gacttcaatg cagacctaac aaaagaagat gcgattttca acgcaatcga
    13261 attagaccac cgcgacatga tcaacgaaga tgaatcagag tttgttcctg tttatcttga
    13321 tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga ttgaaaaaca
    13381 tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg gatttggtga tcttgcgtat
    13441 cgcgattttt gaaatgactt atgtgacaga tgtaccagcg gctgttgctt taaatgaagc
    13501 aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca acggtgtttt
    13561 atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt tctatgaatc
    13621 atcataaacc tgttataaat cgggaaaatt ctatggtaga atgaaaacac aaaaaatatc
    13681 tgggagtggt tagatggcag aattgattaa cggaaaagaa ttagctgaaa aaatgcaggc
    13741 agagattgct gaaaaaatcc aaaaattgaa agataatggt atccatcccg gattagtcgt
    13801 tttattggta ggggaaaatc cagccagcca aatctatgta agaaataaag aacgtgtagc
    13861 aaaagaaatc ggaatccatt cattggtaga gcgttatcct gagacgattt ctgaagacga
    13921 actacttgca gaaattgaaa aatacaatca agatcctagt tttcatggta tattggtcca
    13981 attaccattg ccaaagcata tagatgaaga aaagatcctg ttagctatcc gccctgaaaa
    14041 agacgtagac ggttttcatc cgatgaattt aggcagactc cttgcgggta aaccggacaa
    14101 gattccttgt actccatacg gcatcatgaa aatgtttgaa gcttattcta tcgatccggc
    14161 gggtaaacga gcagtagtta tcggacgaag caatatcgtc ggtaaaccga tggctcagct
    14221 actcttgatg gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa atctggctga
    14281 actggcacaa gaagcagata ttttagtcgt agcaatagga cgtggacatt ttgtcacaaa
    14341 agaattcgta aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg atgctaacgg
    14401 taagctgatc ggtgatgtga aatttgatga agtggagcct attgcaggct ggatcactcc
    14461 tgtgccaaag ggtgtagggc caatgactat cacgatgctg atgtatcaaa ccgtaaaatc
    14521 tgccgaagct tttggagcag gtgaatagat ggcacaagaa tatttaactg tcactacatt
    14581 aacgaagtat ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg tatatttaac
    14641 aggcgaaata tcgaatttcc gtttacgtgc caatgcccat caatatttca gcttaaaaga
    14701 tgaccatgca aaaatatctg ctattatgtt caaaggagca ttccaaaaat tacgattcca
    14761 gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg aagcgggagg
    14821 ttcttatcaa atctatgtag agcacatgga gcctgatgga ataggtgctt tatatcaaga
    14881 attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc ctaaaaaaat
    14941 gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag cggttatccg
    15001 cgatatcatc acgacggtga aaagaagata tccaattgtc caattggttt tgttcccaac
    15061 agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg aatatccaga gagtagaaga
    15121 gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg aggatttatg
    15181 gccgtttaat gaagagcggg ttgctcgggc gatttatcaa gctaatacgc cggtgatttc
    15241 atctgtagga catgaaacag ataccacgat cgctgattta gtagcagatg ttcgggctgc
    15301 cactccaaca gcagctgcag aattagctgt tcctgttttg tcagaagagt tgatgcgcat
    15361 tcaagaacgg caagctagat tgcagcaggc gtttgtacgc cagatccagc gcaaacaaga
    15421 acggttcgag cgtgctcgac gttcctatgt atttcggcag cctgaaagat tgtatgaagg
    15481 tcagaccatc aaattagacc agttgaaaca gcgattatat caagctacac agcaaattta
    15541 tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag tggctccgct
    15601 ttatcgagtt aaagcagctc gccaagaaac tgcttatcta gaaaaacgct tagaagagaa
    15661 aatccaacaa tatatgaaac aacagcagca gcgttttgct caagctgttc aatctcttga
    15721 tttgttgagt ccattgaaaa tcatgggaag aggctacagc tatacgacgt taaatgatca
    15781 agtagtaaaa agtgtcaccg agattcatca aggggatttg ctgacgatcc attatcaaga
    15841 tggaatcgtt aaaggacaag tacaagagat agacaaagga gaataaaaat atggaaaatc
    15901 caacttttga agagtcactg caagaattag aaaaaattgt catgcagtta gaacaaggag
    15961 acgttccatt agaatcagct ctagattcgt ttaaacgagg aatggaatta agcaaacatt
    16021 gccaagatac attagctaaa gcagaagaga ccttgacgaa aatgatgaca gaatcaaatg
    16081 aagaagttga ttttgacgga aacgaggaaa gctgagatga gattaactga cttttcagct
    16141 attcatctac ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca tacaacggat
    16201 agaggactct atgatgcgat gagttattca gtgaaagcag gcgggaaaag aatacgtccc
    16261 ttattacttt taacggccgt tgcttccttt gatgagccta ttgatgttcc cgtctatcaa
    16321 gtagcagctg ctttagaaat ggtgcatacg tattccttga tccacgatga tttgcctgcg
    16381 atggacaatg atgatcttcg tagaggaaaa cctaccaatc ataaagtatt cggcgaagca
    16441 ttagctatat tggccggaga tggtttgctt acaggagcat tccagctaat cagtatggca
    16501 cacttaggta attctcctaa gcttctgctg cttcagcaat tagctgtttg tgcgggatca
    16561 caaggtatgg ttgctgggca agctgcagat atcgagggcg aatcgaaaaa gctttcttta
    16621 gaagaattag catttattca tgaaagaaag acagggcatt tgattcgtta tgccttgctg
    16681 gctggtggca tattagcgaa gcaacctgaa gaaatcttat tgctacttca acgattagcc
    16741 gaacatttag gtttagcttt tcagataaga gatgatctac tcgatgtgat tggtacaacg
    16801 aaaacattag ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac ttacccgcgt
    16861 ttattaggcc tagaaaagac acgtgaggcg ttggagattg agctgttatc cgccaacaag
    16921 atcatagaca aattagaaga ggacgttttt tcttttgatg gcgggctgat gcgccagatg
    16981 atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc gtagatgtat
    17041 tagcggtcaa acaaggatta tttgaaacaa gagaacaagc caaacgttca gtgatggctg
    17101 gtttgattta caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa attccagtga
    17161 gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg ggattaaaat
    17221 tagaaaaagc actcaaagca tttgatctat ctgttgaagg aaaaacattg ttagatattg
    17281 gtgcatccac tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa atgagttatg
    17341 ctttagacgt tggctacaat cagctagctt ggaaaataag acaagatcct agagtcgtcg
    17401 taatggaaag agtgaatttt cgttatgcaa aacccgaaga ctttacctta ggaattccag
    17461 aagttgctgt tatcgatgtt tcttttattt cactaaaact gatgctgcct ccattacatg
    17521 ctattttaaa agaagaggga gaagtgattg ctttaatcaa accccaattc gaagcaggca
    17581 gagaagctgt agggaaaaac ggaattgttc gtgaccctca gacccatcaa aaagttttag
    17641 aagacatttt gtcatttgcg gcacaaggtg gatatgatgt gctggaatta agttattctc
    17701 cgatcacggg gggagaagga aatatcgagt tcttggctca cttgaggaaa gtacccgaaa
    17761 gcggaacaat caattcagct attaatatgg cagaagtggt ttcaaatgca cacgaacagt
    17821 ttgaccataa ataaattgta actgtgaaaa tcaagtgaga agtatgcgga ttcttcttta
    17881 ttataccaag gaaataagct atcattgatt aagaagaata tgctaaggag cgaagacgta
    17941 atgagaaaaa aagatcggca tcgtttgatt actcgattgt tgagtgagca agatatccga
    18001 aagcaggaag aatttgttga aatcctgaaa agcaaaggaa tatctgttac tcaagctacg
    18061 atttccagag atataaagga actgaaactg atcaaggtac cggcttcaga tggcggctac
    18121 cgttatagca taccagctga aacaaacgaa gatgtaaata ctaaattgga aaaactatta
    18181 aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa aacattgccg
    18241 ggaaatgctt cggcagcagc aaatttgatt gataaacgtt acaagaaaga attattttcg
    18301 attatcaatg atgacgataa tgttttgatg attacacgaa ctgaagacca ggcagtttta
    18361 ttgaagaaag acttcctgca ttacctttaa gttgaggagc gaatgtgatg ctacaagaaa
    18421 tcagtattac taactttgcg attattccgg aattgcggct ttcttttcac gaaggaatga
    18481 ccgcgctgac cggagagact ggtgcgggta aatccattat tatcgatgct ttaggtctgt
    18541 tagcaggagg aagaggttca agcgattata tacgtcaagg agcagaaaaa tgtgtgcttg
    18601 aagggttgtt tgaactacca aaacaagaag gattctctga actgatggtt gaattaggga
    18661 tcgaaacaga tgaggacaat ctgattgttc gacgggatat gtccttaact ggaaaaaatg
    18721 tttgtcgagt gaatggtcat attatcacat tagctaatct gagaagaatc ggcagttatt
    18781 tagtagatat ccaaggacaa aacgaacatc aggaactgct ccagcctgaa tcacatcttg
    18841 ctttattgga tcgttttgga gatgtagctt tccaacagaa gaaaaagagt tatcagcaag
    18901 aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa aatgaaaaat
    18961 cctatgttca gagaatagat atgctccatt tccagcaaga agaaatagct gctgctgatt
    19021 tgcaagtcgg agaagaagag aaattaaaag aagaaagaga aaaacttagc aattatcaaa
    19081 aaattgctga tggattagct gcaggttacg gagcactgac gaataatgaa caaaacagtg
    19141 tagatggtgt tggcttggcc gtttcagaac tgcaagggat cgcccatcta gatgtggagt
    19201 atgaagcaat ttatgaaaat atccaaagtg cttactattt gctgcaagat gcaatcggcg
    19261 atatgagtcg tcagattgat ttattggagt tagatgagag tcgtttagaa gaagttacgc
    19321 aacgtttaga attaatccga cagttgaaac gtaaatacgg cgaatccatt gagtcaatat
    19381 tggcttacta tgacgaaatc accgaagagc tagcttcttc tgatttttca gaaggtcaat
    19441 tggacaaaat gaaagaggaa ctagagcaaa aagagcttct acttcagcaa caagccgcag
    19501 atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt ctgcatgaat
    19561 tgaaaagtct gtatatggaa aacactgagt ttgaggtacg tttcttaaaa gaagaaaata
    19621 gacaattgaa tcatgatgga tttgaccaaa tcgaatttta cattacgacc aatccaggag
    19681 agccactgaa gcctttggtg aaagtcgctt ctggtggcga actgtctcgg atgttgcttg
    19741 ctttgaaaac tattttttct tctgagcaag gtgtgacaag tattattttt gatgaagtgg
    19801 atacaggtgt gagcggaaga gttgcacagg ccatcgcaga taaaatttct caaatatcga
    19861 aatactctca agttttgtgc atcactcatc tgcctcaagt tgctgcagtc gccgactatc
    19921 aatattatat tgtgaaagca gtgattgatg gacgtacgca aacttctgtt tcagagttga
    19981 aaacaaaaga aagagaagaa gaaattgcac gtatgctggc tggaagtgag attacaaaac
    20041 tcacactcga acatgcaaaa gaactattga aactcgcaaa aaggacctga gccacctcgc
    20101 agtggttcag gtccttttca gcagctgata gctatatttt atttttacat gacactcatt
    20161 acagcagtca atacaacaga tccaatcgtt aaaatcagca ttgtccaaat gacaaatttt
    20221 gttactttag caaaagtact tgtttgtttt ttttcacgca ttgttttcaa cctcgtcatt
    20281 aattagttga ttcaagtgta caatcttttt tccatttcca caagagggct tttttataaa
    20341 aacttccctt tttttaaata ataggtagtt acgatacaaa taaataatgt taatagaact
    20401 agccatataa aggcatcatc cctttcagca ataggtaact taatgttcat tccatagata
    20461 ctgccgacga ttgtcggtat agttaaaata atcgttaaag aagttaaaat tttcataata
    20521 ttattcagat tattcgatac gattgctgaa aaagtctgat tcatttgttc tactagtttt
    20581 aattgaatgc gagaagtagt cattgcctgc tctgtttcca ccaatacatc acgtaaatga
    20641 tttttcaaag catgattttc ttggaaaagt ttattttgcg ctagttcttt caatgcatct
    20701 aaattagcag aagtcgcttc tctgaaataa acgaggcttt tttggatatc catgatttga
    20761 taaagttgtt tgttttcagt ggatacttga agtttggctt ccaaagtatc gcattgagat
    20821 tgaagcgtag ctaaataact gttgtaagag agtgtgacct gccaaatgaa ttgaaagaag
    20881 atattgagat taggcgaaat atcacttttt ggaaaggtct ggctaaagac tttctttaaa
    20941 aacagtggtt catgattcgt aattgtgata agcttctttt tgggcgtcaa aataatagag
    21001 agcgggtatg tatccacttg aaaataaccg ctaggacttg ttttagcaaa aggatattgt
    21061 aacaataata acgctggctt tgtcaacttt gattgattga attcttctgt acgggaattt
    21121 tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa tttcaaattg atgtgtcagc
    21181 tgatgaatct cttcttgatt aggacgttct acacaatacc agatcgtttg gtcagtttcc
    21241 tttccggtat tacgcagttt ctcattatct aatgtaaaat atttgatcat gattttcctc
    21301 ctgttgcctg tttcttttat tataacggat agggaattgg aatacatgga atgtgtttgt
    21361 aagatataga atataactgc ataaatttaa ataaaatgaa acagttgtgt aaagtatata
    21421 cagtatactt gaagcaacga taaaaaagga gtttacgttc atgggcacat tcacaccact
    21481 tatcttgttt tggtatattt acccggtaat cgttttgttt ggttgccaat ttcttgtaaa
    21541 attattttcg ttaaatcgtc gttttaaaat aaaagcccct gatttggcga ttcctttttt
    21601 gtggggcggt atgcatgcat tgtctagaaa tacagggaca atttctatct taccgttttt
    21661 ctttatttct gttttgctaa tgggaatttg tattgctgtt ttccaagcat attattatga
    21721 agaaattatt tatccgcgtt atttcaagat gacttggcgt ttggtttttc tgttgacaat
    21781 cgtcttgtac gctgtcttga tcattgtgaa catactttct tacctatgaa aaaaatagaa
    21841 aagctgtaaa aagtctttta aaaggctttt tgcagcttga tttttaaaaa aatgtttatg
    21901 aataaaaaaa gttgttcccg gatttcccag aaaaggtggt agaaagtggg ggattgtggt
    21961 agactaattt tagttagtgg aggaatgggg ggctttcaat gttgatgggt gaattccaac
    22021 ataatattga tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg
    22081 aaaagtttgt tctgacacgc ggattggatg gctgtctttt cggctatccg atgagcgaat
    22141 gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt
    22201 ttgtgcgatt cttctattcg gctgccactg aatgcgaact tgataaacaa ggacgaatca
    22261 atatacccag cacgttaaga aattatgcag ctttgacaaa aggttgcgta gtcatcggtg
    22321 tatctaaccg aattgaaatt tgggatgaga cacgatggca agatttttct gcagcagcag
    22381 aagagaactt tgacgaaatt gctgaaagta tgattgattt cggactataa gaataggaag
    22441 aggctgacta atgactgaaa catttcaaca ctatacagtc atgttgaaag aaacggtaga
    22501 tggattgaac atcaaaccgg atggcgttta tgtagactgt acactcggcg gtgccggaca
    22561 tagtgaatat ttattaagcc aactaagttc taaagggcat ctgtatgcgt ttgaccaaga
    22621 tcaaaaagca atcgatcacg ccaagctgcg tttagcttct tatgtcgaac aaggtatggt
    22681 cacatttatc aaagcgaact tcagagaatt agaagaacgc ttgagtgagc atgtagataa
    22741 ggtagacggc attttgtatg atctaggggt ttcctctcct cagctagatg aagcagaacg
    22801 tggatttagt tatcatcaag atgcaccgtt ggatatgcgc atggatcagt cagctccttt
    22861 atccgcttat cacgtaatta atgaatattc ctaccatgaa ttagtaaaga ttttcttccg
    22921 ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac
    22981 aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc
    23041 cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt caagctgtgc ggattgcagt
    23101 aaatgacgaa ctcggtgccg aagaggcctc tttagaacag gcaatccggt tattgaaagt
    23161 caatggaaga atcagcgtaa tcacattcca ttcattggaa gacagaattg tcaaaagtat
    23221 gtttaaagaa tatagtacgg tacaagatct accaccggga cttcctgtca tacctgacga
    23281 attccaacca gaactgaaag tcattactcg taaaccaatc cttccttcag aggaagagct
    23341 aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa
    23401 agaggagtag aatgatggca gaattaaaga aaatgcaaga ttacgaatat gatttacctg
    23461 aattggtgga tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc
    23521 ttccacaatc accggcaaaa cgattaaagc agatttctgc tttggaaaaa attgctgtgg
    23581 caagcgtggc gattgctatt gttgctttat gtgttttaac agttatgctg cgcacaaata
    23641 tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag
    23701 aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg tactgaacgg attaaacaaa
    23761 tcgcagaaga aaaaggattg tctattaatg acgacaattt aaggaaagtg aagtaaatga
    23821 gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg atgaacaatc
    23881 gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg attgttcttt ttatttgccg
    23941 tccgtttctc gtatatcgtt attggaggtc atgttgctgg tacctcatta gctgaaaaaa
    24001 caaaacagtt gtaccaagga agtgaagtag taaaagcaaa aagaggaacg atttatgatc
    24061 gtaacggtgt ggcgctcgca gaagatgcct cctcttattc aattaaagcg atactctcaa
    24121 aaacatatac ctctggggat aaaaaactgt atgttgaaga aaagaatttt gataaaatag
    24181 cagaaatcct gcataagaat ctttctatag acaaaaaaga tgcactgaat attttagagg
    24241 acggcgcaaa aaaagagctg tatcaagttg aatttggtag ctatgggaaa aatatctccc
    24301 aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt
    24361 ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt ttcttctcat tttattggat
    24421 acgcagttcc agacaaagat gagaatggct tagttggtaa actaggtttg gagtctgctt
    24481 acaatgatat cttgagcggt aaagatggta agattattta tcaaaaagat aattttcaaa
    24541 atcctctgcc aggtacagtg gcagaagaag aaaaagctgt cgacggacaa gatatctaca
    24601 cgactttaga cagcagacta caaagttatt tagaaacact gatggatcaa gtaaatgaag
    24661 aatatcagcc agaagaattg acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg
    24721 caatgggaca acgacctact ttcaatcctg aaacaatgga aggattgact ggaaaagatg
    24781 ctatctggcg taatttcttg gtacaagata gttatgaacc aggatcaacc atgaaagttt
    24841 tcactactgc agctgctatc gaagaaggag aattcaacga gaatgaaacc ttccaatctg
    24901 gaaaaatcca agtagaagat gctacgatca atgaccatga ctttggtgaa aaaggtgtgc
    24961 tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc
    25021 gattaggcgg acgatggtat aactatttac aaaaactggg atttggacaa agtactcatt
    25081 ctggattaga tgatgaagta aacggtgctt tacctacatt aaatattgtc gaccgagcaa
    25141 tgagtgctta tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa gctttcactt
    25201 ctattgcgaa taacggtaca atgattcaac ctcgatatat cagcaaagtg gtggatcccc
    25261 aaactggaga agagcgaaca actcaaacag aagttctagg gcaacctttt tcaaaagaaa
    25321 caacagaaaa agtccgtgaa tatatgagag atgttgtcga aagcgaaaac tacggtagtg
    25381 cttatggtgt atatagtgta cctggataca atgtatcggc aaaaaacgga acagcccaaa
    25441 tcgcttctga tacaggggga tatcaaacag gagatacagc ctacctgtat tcgattgtgg
    25501 agatggttcc ttctgaagat ccagactatg tcttgtatct aacaatgaaa caccctaaga
    25561 catatgatcg aatggcattg gctaaaatcg caaatccatt gatgaaacgg gcaatggatt
    25621 ttaaagaaac tgaagaagat tcagatacag aaacaaaaac agaaaaagta tccgtagccg
    25681 attatcgtaa tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac
    25741 ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt
    25801 tgatctctgg agaaaaactg atcctatata ctggtggtga taagctgatg cctgacgtaa
    25861 caggatggtc aaaagcagat atcatgaagc tggggaaaat cctcggtatt gaagttagtt
    25921 ttgatggaga tggttattgt gtcaaacaag aacttgctcc atatgaaaaa atcactgaag
    25981 acaaacttag ttttacttta gaagaataag aagagaaata ggagagaaaa gagcatggaa
    26041 tggacacaag cattgattcc cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg
    26101 ctatttattg gttattttca aatgaaaaag caaggtcagg cgattcgtga ggagggacca
    26161 aagtggcaca atagcaaagc cggaactcct acaatgggcg gcttagtgtt tctgatcggc
    26221 tctattttga caggaatttg ggttggagct tggcaaaaac agctaactcc tactcttttc
    26281 atcttgcttt ttgtattagc gttatacgga gtaatcggct ttcttgatga ttttataaaa
    26341 atattcaaga aaagaaacat gggactgaac tcgaagcaga aattgattgg acagattcta
    26401 gggggaatca tcttttatct tgtttaccgt tctgaaggtt atccaggtgt attgaatttc
    26461 ttcggactag atctaccttt aggaatcatt tatggtattt tcgctctctt ctggcttgtt
    26521 ggattttcaa atgcagtaaa tctgacagat ggcatcgatg gattagttgc tggcttagga
    26581 agtatctctt ttgcagctta cggtctaatt gcttggcatc agcatcagta tgatgtatta
    26641 atcatttgct taagtgtttt aggaggttta ctgggtttct tcgtctacaa cagaaaacct
    26701 gccaagattt ttatgggaga tgtcggttca ttagcgttgg gcggactttt agcggcaatt
    26761 tctataatgc taaaccaaga atggacattg cttttgattg gtttgatcta tgttatggaa
    26821 acagcaagtg tgatgcttca agttacttcc tttaagctta caggaaaacg aatttttaaa
    26881 atgtctccaa ttcatcatca ctttgaaatg tgcggatggt cggagtggaa gattgataca
    26941 gttttttggt tgatcagcat tgtgacttcg ttgatcacat tatggatcgt ttggtaggag
    27001 atgacgggaa atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg tcttaggact
    27061 cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt
    27121 taatgatgga aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt ctttagggat
    27181 aaaagtaata acaggtagtc atccaatcga attgttggat gaagaatttt cattgatggt
    27241 caaaaatcca gggattccct acagtcatcc tttagtagca aaagcccaag aaatgggtat
    27301 tccggtaatt acggaagttg agctagctta cgaagtagcg gaatgcccaa ttattgggat
    27361 tacaggaacg aacggaaaaa caacgacaac aacgatgaca ggactactac tgaatgcagg
    27421 agcagatcaa ggaattgcac gtttagcggg taatattggg tatccagcaa gtggtgtagc
    27481 gcaagaagca aaatcagaag ataaaattgt gatggaattg tctagcttcc aactcatggg
    27541 aatcacagat ttccgccctc atatagcggt gattaccaat atctatgaag cacatattga
    27601 ttatcatggt acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac
    27661 agaaaaagat tatctgatcc ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag
    27721 aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc
    27781 tgacgattac tcaatatact ataaaaaaga aaaaataatg gaaatatctg aacttggcgt
    27841 accaggtaag cataatgtag aaaatgcact agctgctatt tctgtagcaa aattgtatgg
    27901 tatatcaaat gaagctatac gagaaacgtt gcattttttc catggcgtac cacatcgtac
    27961 gcaatatgta ggagaaatcc aaggcagaaa attttataat gattcaaaag caaccaatat
    28021 tttagcgact aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt tattggctgg
    28081 cggattagac agaggaaata catttgatga attaattcct tcattaaaag gaatcaaagc
    28141 aatggtcgta tttggccaaa caaaagaaaa gttgatggat gctggaaaaa aagcaggcat
    28201 cgagacaata gtaacagccg attctgtaga acaagcagtt cctttagcat tagaaaacag
    28261 tacagatggt gatgtagttt tattatcacc agcgaatgcc agctgggatc aatacccaaa
    28321 ttttgaaact agaggaaaca gattcatgga agctgtgaac cgattaaaat agaaaaagta
    28381 gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg ggacatattt
    28441 atccagctct tgcctttgtc aattatgtaa agactcaaga accgaatgct gaattcatgt
    28501 atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga atgcctttcc
    28561 ataccttgga gattcaagga tttaaacgaa aaatttccat gcataacatc aagacggtcc
    28621 gattgtttct taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt caaccagata
    28681 tcgttatcgg tactggagga tatgtttccg gagcggtagt gtacgctgca gccaaaatgg
    28741 gtattccaac agttatccat gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa
    28801 gccgatatgt ggatcggatc gctctttcat ttaaagatgc ggcttccttt tttcctgaaa
    28861 acaaagcagt tcttgtaggt aatcctcgag cacaagaagt agcagatact aaaaaatcag
    28921 atattttgaa aacatttgat ttggatccag aaaagaaaac agtcttgatt tttggcggta
    28981 gtcaaggagc acttaaaatc aatcaagcag tatcagtttt tttaagtgaa tatattcctt
    29041 cttcttacca agttttgtat gcttcaggag atagatatta tgaagagatc ctaaataaaa
    29101 taaatcctga aatacaaaat attagtgtcc agccttatat caaaaatatg gcagaggtca
    29161 tggcttccag tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag
    29221 ctctggggtt accggcagtg ttggttccca gcccatatgt tacgaatgat catcagacta
    29281 aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat gattgcagat aatgaactaa
    29341 caggtgaatc actaagtcag acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc
    29401 aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact
    29461 tagtaaaaca aataatcaaa aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc
    29521 aaaaaaattg tctcatccgt cttctgatga aaacaatttg actccttggc aaaaggctaa
    29581 ccgaaaatat ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag aagacataac
    29641 attagataca cccctttcag attctgaaga agaaaaaacg gttcaagagg aacagcaaga
    29701 agaaagccag tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg
    29761 tggaccatat aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa
    29821 agtactattt cgtcggttag ctttgatcat cactgtattg ggtataccgc tgattttttt
    29881 gatctattat gtttctcctt atagcaaatt acaagcagtt gcagtatcag gcaataagac
    29941 agtcaacagc caagaaatca tctctgatac aaaattgtca ttaggagaaa atgtttgggg
    30001 acagtatttt catcgatcta cttatatcga tcgccttaaa aaagcacagc caagaatcga
    30061 aacagcaaat atacatttca aagggatgaa tgaattcgat ttggatgtaa cagaatataa
    30121 agagattgct ttgatcgcaa aaaacaatca atactaccca gtgattgaaa atggaacagt
    30181 attagatgaa aaagtagcaa atcctacaaa aaatctccct atcttggaag agttcaaaga
    30241 ctctgcaaaa atcaaagaat taacaaagca atacaatcag ttatcatcag aattacaaaa
    30301 agcaatttct gaaatcaagt acacgccaag agcaagcaat aaaaatctga tccaattgaa
    30361 tatgaatgac ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc aaatgaaata
    30421 ttattcgcaa gtagccaaag atatggatga aaaaggtgtg atcgacatgg aagttggtat
    30481 cttctcgcat ccttacggta gcactcaaga aacggaagaa tcgggccaat cggaagaaac
    30541 aactgaaagt gaagaaaatg cggatcaaac aaacgaatca agtgaagagg cagaaacgca
    30601 aaaagcacaa acacctggat cgtctagcac aaacggatca tctgattcaa atgattcaag
    30661 tgaaacaagt caaatagaag ataatccaag tacaactccg tcaagtttaa atgaaaattg
    30721 ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt atggtagaat
    30781 tggaagcagt gaatatatct attaataagt atattttaaa acggacgcta gttgttcgct
    30841 tgtatatgga atagtaagta ggagggaatc ccattcatgg caaaaacagg aatgtatgta
    30901 ggccttgata ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa
    30961 atgaatatta ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt
    31021 gatatcgata aaacggtcca agcgatacaa cgagcagtac gacaagcaga agaaaaagca
    31081 ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga agtagaaaat
    31141 tgccagggaa tgattgcagt taacggagat tcaaaggaaa tcaccgatga agatgtcaga
    31201 aatgtggctt cagctgccct tgttcgttca atacctcctg aacgtcaaat cgtgtcaata
    31261 ttgccacaag attttactgt cgatgggttc gagggaatca aagatcctcg tggaatgatc
    31321 ggcgtaagat tagaaatgta cggtctgttg tttacaggac ctaaaacgat catccataac
    31381 atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat tactccttta
    31441 gcgctgactg aatcaattct ttctgacgga gaaaaagatt ttggtacgat cgtcattgat
    31501 atgggtggcg gccaaacaac aacggctgtt atgcatgata agcaattgaa gtttaccaat
    31561 cttgaccaag aaggtggcga atttgtcact aaagatatct ctattgtatt aaatacgtca
    31621 tttaacaatg cagaagcgtt gaaaataaac tacggggatg catacccaga acggacatca
    31681 ccagatgaag aatttccagt agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa
    31741 cgctacttgt cagaaattat ttcagctcga atggaacaga ttttcaacaa agcaaaagat
    31801 gcattggacc aaatcgaagc attagagttg ccaggtggcg tcgtattgac aggcggagct
    31861 gctagtcttc ctggtgttgt ggacttggca caagaaatct tcggcgtaaa tgtaaaattg
    31921 tatgttccaa atcacatggg gctgagaaac ccggtattta caaatgtaat aagtatcgtg
    31981 gattattcag caaacttgag tgaagtgtat caattggcaa aaattgctgt tacgggtgaa
    32041 acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa caaacacata tgagagctat
    32101 gaagcacctg aagaaacaat ctacgatgag ccggaaccaa aagagtccgg agaaaatgta
    32161 aaaaataaaa tcaagggttt ctttacaaat atttttgatt aattcatcct gggaggaata
    32221 agatatggaa ttctcaattg ataataatat caatgacggc gcagtaatca aagtcatcgg
    32281 agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt
    32341 tgaattcatt acagccaaca cagacgtgca agcattaaaa aattcaaaag ctgaaacagt
    32401 tatccaatta ggccccaaat atactcgtgg tttaggtgct ggttctcagc cagaagttgg
    32461 acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg gtgcagatat
    32521 gattttcatc actgcaggaa tgggtggagg aaccggtaca ggtgctgcac caatcgttgc
    32581 aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc cattcacttt
    32641 tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc gctcgtttga aagaaaatgt
    32701 ggatacatta ttaattatct caaataaccg tttattagaa gttgtagata aaaagacacc
    32761 gatgcttgaa gctttccgtg aagcagataa tgttttacga caaggtgtac aaggtatctc
    32821 tgatttgatc acagcgccag gttacgttaa ccttgacttt gctgacgtga aaacagtgat
    32881 ggaaaaccaa ggtacagcat tgatggggat cggtgtggct agtggagaag atcgcgtagt
    32941 agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa acatctattg atggagctga
    33001 acaagtactt cttaacatca caggcggttt ggatatgaca ttgttcgaag cacaagatgc
    33061 ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat atcattctag gtacatctat
    33121 caatgaagaa atgggcgatg aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc
    33181 aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca caaatgcaat cacctgctca
    33241 aaagactgta ttagatatgg atcaagctaa accaacttct tcagaagaag aaaacagttt
    33301 tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct agagttgatg attccaattt
    33361 tgacaatatc gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa aatctcatgg
    33421 cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga gaggaagagc
    33481 acatgattgc tgacaacttg cagaaaatca agcaagaaat tcaggattcg tgtgctcttg
    33541 ttcatcgtga tccgaaagaa gttacattag tagctgtaac aaaatctgtt gaatccgctc
    33601 aagctgaaga attggttaga gctggcgtta tgaattgtgc agaaaaccga gtagataaat
    33661 tattagaaaa gaaacaagat ttatttgatt ttcctgaaat aaagtggcat ttaattggta
    33721 atttacagcg cagaaaggta aaattgattg taaatgaaat tgattacttt catgcgttgg
    33781 acagtttaag attagcagaa gaaatccaga agcggttaga gaaagaatta tcctgtttta
    33841 ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat ttcaccagat gagcttttac
    33901 cgtttgtgga atctttagct gcctgcacaa aaatcaaggt tgttgggttg atgactatgg
    33961 cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc
    34021 gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta agtatgggga
    34081 tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac atttatccgt gtaggaacag
    34141 cgctattcaa agacgaataa aggaggaaca aaaatgccgc ttttaaacag aggagcaatt
    34201 gctaattttt tcggattagc tgatgaggaa gagtacgaat accaagatta tcctgaaaca
    34261 acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac agaatcaatc aactcattct
    34321 gtatctgctg cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat
    34381 gaaagtattc agccggctga aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa
    34441 actgtagcga aagaaagaac agcttcaaga acctctacac aacctaaagc aaatcttgct
    34501 ggaaaaatca ctgtaatgga accaagagct tattctgaag ctatggcgat tgctaaacga
    34561 atcattgctg gagaagcaac cttagtgaat tttcatttag tagaagaaaa acaagcgaga
    34621 agaatcgttg attttctaac aggaacagtt tatgcactag atggagatat caaacgtgtt
    34681 ggagatgaga tttttctttg tacaccgact gggatggaga ttgacagttc aaccgctcaa
    34741 tcttttgcag atgcgaattt gtttgatctg taagaggagg aactacgatt tatactattg
    34801 cactattact tcatttactt caacgggcag cctatttcta tagcattatc ttgatggtat
    34861 atgcattgct ctcttggttt ccaggcgggt atcaatcagg gctaggacga ttattagcta
    34921 agatctgcga accgtatatc agtttgttcg atcgtctgcc tttgaggatt ggacccttgg
    34981 atttcacaat tttaggggca atcctgatct tgaacttagc cgtacaaggt ttagcacaga
    35041 tcgttatatg gataactgtc catttagttt attgattgaa gcaacaaaat ctttattctc
    35101 agaatagata ggaggggaaa attttggatg caaatgtata ccagcacttt cgggcagatg
    35161 aacgtacatt tatcgattcg gtaggcgatt ggattgaaca ggttcaagga caatatgcac
    35221 cctatctgac cgaatttctt gacccaagac aatcctatat tttagaaaca ctgattcgac
    35281 aaagcagtga tttgcgtttc atgttttatg gcggatatga acaggcagaa agaaaaagat
    35341 gtttgatttt ccccgattat tacgaacctg tagaggaaga ttttgaaaca gcattgtatg
    35401 aaatccatta tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt ggaactttag
    35461 tgggaacggg gatcaaacgc tcttactttg gcgatatcat ctcggacggt gatcgctggc
    35521 aagtatttat tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca aagattggaa
    35581 aggttgctgt acgcttggaa ccaatcaaat acacagaaat gattatgccg aaagatagct
    35641 ggacacaaga aagaggaaca gttagttcca tgcgactaga cagtgtgatc tctgagattt
    35701 ataatatttc cagacagcgg tccaagcagc tgatcgaagc tggtaaagta aaagtgaact
    35761 ggacagaaaa tacaagacct gattttgtac ttgaactatt agatattatt tcagtcagag
    35821 gttatggcag agtccagatc caagaacttg aggggaagac gaaaaaagaa aagtacaggg
    35881 tactgttcgg tgtattacgt aaataaaata aaaaggtggt ttactcatgg ctttaactcc
    35941 attagatatc caaaataaga cattcccgac aaaaatgcgg ggatataatc aagacgaagt
    36001 agatgatttc ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga
    36061 attagaaaaa gcagtcaaac attctgaaga aaaacttgag tacttcaatg aattaaaaga
    36121 tgctttgaac caatcaatca tcgtagctca agatacggct gataaagtaa aaacaagtgc
    36181 aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac aaagcagatg agttagttgc
    36241 gaacgctgaa aaacgtgcac atcaattaac aactgatgca gaagaaaaag caagaaaaat
    36301 cttgacagat gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag atcttaagaa
    36361 aaaaacacga gtattccatc aacgtatcag cttgatgctt gagtctcaat tagaacaagt
    36421 gaaaagtccg gaatgggatg aaattttaca accattctcc agctacgtga ctgacagcca
    36481 tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa
    36541 ctcagacaca ggagtcatag aagcacctat gacggcattt gatacacagg ctattgatat
    36601 ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga aaagcacttt gttattctta
    36661 aagcgagccg atgagagtgg aagttcggta agacactgca tggtgaaaga tcctctactt
    36721 gagacctgct tttccgaaat tagagtaaga agcagcggct gacctcgtta ttggtcacag
    36781 agagtttgca ctagatagat gcaactaaat ttgggtggta ccacggcatg ttcgtccctt
    36841 gcggctgaac atgctttttt gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa
    36901 aatgaaagaa accttgcaat taggaaaaac aaaattccct atgcgtggta atttgccaaa
    36961 ccgagaaggc gagtggcaaa aagaatggga agaaaatcga atctaccaaa aaagacaaga
    37021 gttgaacgaa ggaaaaccta gctttgttct tcatgatgga cctccatatg ccaatggtaa
    37081 tatccattta ggacattcat tgaataaaat cagtaaagac attatcattc gttcaaaatc
    37141 aatgtctggc tatcgtgcac catatgttcc aggatgggat acacatggat tgccgattga
    37201 acaagtattg gctaataaag gcgtgaaacg taaagaaatg tccatggcag aatacttgga
    37261 aaaatgtcgt gaatatgcac tttcccaagt agataagcaa cgagcagatt tcaaacgcct
    37321 aggagttgca ggagattggg aacatcctta tgttacatta gatccttctt atgaagctgc
    37381 acaagtacgc gtatttggta aaatggctga aaaaggctat atttacaaag gattgaaacc
    37441 aatttactgg tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg aatataaaga
    37501 tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa gacggcaaag atattcttga
    37561 tgaagataca gcttttgtca tttggacgac aacaccatgg acattgccag caaaccaagg
    37621 tattgctgta aatccaaaat ttacgtatgt attggtggaa gccgatggcc gtaagtttgt
    37681 cgtagcgaaa gatttgctag aaacagtcca atcagaaatc ggttgggaaa atgtaacagt
    37741 tctaaaagaa tttgctggac aagaaatgga atatatgact gcaagtcatc cattctatga
    37801 acgtgaatca cttgtgattt taggcgatca tgttacttta gacgctggta ctggattagt
    37861 ccatactgct ccaggacatg gggaagatga ctatatcgct ggtagcaaat ataaactatc
    37921 tgtagtgtca cctgtagact caaaaggatt gtttacagaa gaagcacctg gatttgaagg
    37981 tatcttttat gacaaagcaa atcctatgat cactgatcta ttaaaagaaa aaggagcttt
    38041 gctgaagcta gatttcttta ctcatagtta tcctcacgat tggcggacga aaaagcctgt
    38101 gatctatcgg gcaactccac aatggtttgc atctatcgat aaattccgtc agaatatttt
    38161 ggatgaagta gaaaaagtag agtgggttat cccatggggt aaaacacgtt tgtataatat
    38221 gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag taccacttcc
    38281 gattttctat gctgaaaatg gagaagcaat catcacacca gaaacaatcg aacatgtggc
    38341 aaaattattt gctgaacatg gatcaattat ctggtttgaa aaagaagcga aagaacttct
    38401 tcctgaagga tttacccacc caggttctcc taatggcgaa tttacaaaag aaaaagatat
    38461 catggatgtc tggtttgatt ctggttcttc tcatgaagct gttttacgtc agcgaccaga
    38521 attatcattc ccagcagata tgtatttaga aggatcagac caatatcgtg gctggttcaa
    38581 ctcaagtatc acgactagtg tggctatcaa tggtgtagca ccatacaaat cagttatttc
    38641 tcaaggattt gctttagatg gtgaaggccg taagatgagt aaatctttag gaaacatcat
    38701 tgcaccagaa aaagtgatca aacaatttgg tgcagatatc cttcgcttat gggtatctag
    38761 tgtagattat gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt
    38821 atatcgtaaa atcagaaata cgatgcgttt cttattagcg aatacagaag atttcgatcc
    38881 taaaaaagat actgtttcgt acaatgattt acgttctgta gacaaatata tgaccgttcg
    38941 attgaaccaa gtaatcaaag aaatccgaga agaagggtac gacaaatata acttcatgca
    39001 tatttaccgt acagttatga acttcttgac ggttgatcta tcgtcattct acctggattt
    39061 tgctaaagat gtagtttata ttgaagcaga agatgattat caacgtcgtt gcatgcaaac
    39121 tgttttctat caaacagctg tagctttgac aaaattattg actcctatca ttccgcatac
    39181 agcagaagaa atctggtcat tcctgaaaga agaagaggag tacgttcagt taagcgagtt
    39241 tcctggttat gaagagtttg caaaccaaga agaattgatg gatacctgga ctgctttcat
    39301 ggacttccgt gataatgtct taaaagcatt ggaagaagca agaaacagca aattgattgg
    39361 taaatctttg gaagcaaaac taactgttta tccaaaacaa caagttcgtg aaatgttaaa
    39421 agctttggat gcagatattg cacaattatt gatcgtttca ccagactact ttgaagttat
    39481 ggcagcagat gaacaagtgc cagataacgc tatggtattt gatgatgttg ctattacagt
    39541 ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt
    39601 agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc
    39661 tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc
    39721 tttgtcacac ccccttttgt tatcaatcgt ttaattttcc acgttcacga taccagttat
    39781 ctggttgcca gatccattcg aatccgtctt tttcaagtag ttcgaaagct gctttaggtc
    39841 ccattgatcc agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa
    39901 tgacatccac gatatgccaa gattgagcga cttcttccca atgagagaag ttcgttccat
    39961 caccattcaa cgcatctaat agtaattttt cgtaagcttc tgggctattt tcaaccattt
    40021 ctgcactatt tctaaattcc aatttgattg gttccgtttc aaatccttgc cctacttctt
    40081 tcccattcaa ggataaagaa aatccttcag ttggctgaat atagatcgtt agaacattag
    40141 gtggaagtgt cgtatcgtca catggttcat cgacagatgt tttgaataca ttaacaggaa
    40201 cttgtttgaa tacgatgttg atccgagtcc ctttttcagt caaacgtttg cctgtacgta
    40261 catagaaagg aacacctgac caacggaagt tatcgatagt gaatttgcct gcgacgaatg
    40321 tttcagtctc tgattgagca tccactttgt cttcttcgcg atagcccttg aattgtacac
    40381 catccaattc accttgagca tattgcccgc gaacgaagtt ttctaacact tcttcttcgt
    40441 tataaatgcg gatagctttc aatgctttga tcttttctgt acggatttct ttttcggaaa
    40501 atgctactgg cggttccatt gcaagtaatg ctacaacctg taaaatatga ttttgcacca
    40561 tgtctttaag tgcgccacta tgatcgtagt agcccccgcg atcttctaca ccgaggtttt
    40621 cgccaaaagt aatttggata ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat
    40681 tattggcaaa acggatagct gagatgtttt ggatcatttc ttttcctaaa taatgatcaa
    40741 tcctgaaaat atcttgttct gggaatactt ctctgatttg ctggttcaat tcaaaagcag
    40801 actggtaatc agaaccgaac ggtttttcta tgatcaaacg attgaagcca ttttttgtca
    40861 taatattttg tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt gccatagcca
    40921 aataatagac acggttaccg tttaattgat agtgtccatc taatcggtca gacaattctt
    40981 ttaatgtctg ataatgcgtt gaatcattta catcatggga ttgatagtaa aaatggcttg
    41041 caaaggtagt tgcttcttca tctgaaggat tcaatgcacg gattgtttct ttgacaactt
    41101 cacggtagtg ctcatcgctc cagggacggc gagcggttcc gattacagca aaatgctcgc
    41161 ctaaatttcc ttttctgtaa aggcggaaca aagacgggta caatttacgc tgggctaggt
    41221 cacctgtacc gccgaatatc gtaaacaata catttttttc attagacatg tggatacact
    41281 cctttatatt ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg ggattttctt
    41341 tctatcattt tggatagtga tcggtccttc ataagctgcg atgtcaacga tctcatattc
    41401 ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac aattcttttt catcaagcac
    41461 tcgtgcgata cgcacttttg taccgacagg ataggactcc aatgtttgtc ggcgttcttc
    41521 atggacaact tcctcttttt taggaatcga tcctccatga ggacaatgtt caggatgatt
    41581 caaatagttt tctaaatgat ctgcaagatg ttccgaagtc acatgttcca atacttcagc
    41641 atcatcatgg acttcattcc agctatagtc taaatgttca actaaaaaaa cttcccataa
    41701 gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg cttgcagtca attgtacacc
    41761 ttgataaggg gaatgttcta caagctcttc tttcacaagt ttagaaatca tttcactgac
    41821 agatgcagct gagacatcta gactagatac gatttgttta ttgttgactt tcgttgtatc
    41881 tccacctagt tctaaaatga ttttcaaata atcttctcga tttggtgtca tattcgatca
    41941 tcctttcatg gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt aattgactat
    42001 ttttctaaat aaaaaacaaa ggatttttag acgtttttac aacaaaaaac gaaagatgat
    42061 agagaagact cattctttcg tttataaagg atatatatat acattgggag ggatctattt
    42121 ttcagataac aacttatatg aagtgtctgc agattcgtca taaagagttt gttcgggata
    42181 agaaaagata agataaaaac catactgatc acgtacgact aaacgatatt catctatttc
    42241 taccacagat gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg
    42301 taattgttgt ttttgctggc tatcactaaa gaaccatgta ccttgatact ttgagagtcc
    42361 ttctgacttt tccgcttgtt ttttcttttt caagtaaata aaacttgaga gaacggtaat
    42421 gagtgaacca agaaaataga tcattttttt tatcaccaaa tcactccttt atgagaaaat
    42481 aatcgcatag acatattctt tcttctttat ttcattgtac cgaataaaag ctaaaatttc
    42541 tctaagactt tcgactggtt tttttactgt ttttctacaa attatgatag gatacatagt
    42601 aggaagaaag gtgacataat ttgaagtatc agcaagtgga aatagctttt tttattgacc
    42661 gcccgaatcg gtttatagca cattgtatga aaaaagacgg agaaataatt attgcgcatg
    42721 tcaaaaatac aggtagagga aaagaggtcc tgataccagg cgctgaagtg gctattgtat
    42781 ttgctcctgg acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg
    42841 actggtttaa tatcgatagt caattgccta atcggctggc aattgatggt attttagacg
    42901 gcacgatcct tttaccagga ttaactggtc catttacggt atacaagcgt gaagtaactt
    42961 atcttcactc caaatttgat atttatttag agacggtggt cggacaaaaa gcatttgttg
    43021 aagtcaaagg tatgacactt gaaaataaag cgattggtgc ttttcctgat gcgcccacac
    43081 tcagaggatt gaaacatata ggagaattaa cgtatgccgc acaagatggt tatgccgctt
    43141 atgtcttgtt tattgctcag ttcgaacatc ttcatctggc aacgatccat gaagaaatgc
    43201 aaccagcact agctgatatg gttcgacatg cgcagcaaag cggagttcaa atcctcgcct
    43261 ataattgtca agtatcggca gatgaggtaa ctgtcaagca ggccattcca tttgatgtag
    43321 atatcccttt tgaagatcct atacaataga aagaaggtaa aataatgatc aacttaggta
    43381 ttatcggaac aaattggatt actcatcaat ttgtccaagc agctttaagc acaaaaaaat
    43441 atgaattgac agcggtatat tctagacatt tggaaaaagc acaggaattt ggttctcatt
    43501 atgaagggga tatcgaattc gcaacagatt tgaagacttt tttcaatatt actcatatgg
    43561 acaccattta tattgcttct ccgaatagtc ttcactttag tcaagctaag caagcaattt
    43621 tggcaggaaa aaatgtgata gttgaaaagc cagctttttc gacgccaaaa gaaatggatg
    43681 aaatcatcca tttggcaaat gaacacaatg tgtatttttt tgaagcagca agaaatatcc
    43741 atgaatcagg ttttaaaaag attgccgatt acttaccatt aaaagacgag ataatcggtg
    43801 caaatttcag ctatatgaaa tattcttcgc gctatgacca agtattagat ggagaagaac
    43861 cgaatatctt ctcgactcat ttctcaggtg gtgcattgat ggatttaggt gtttaccttg
    43921 tatatgcagc tgttgcttgg tttggaatgc caaaagaagt ccattatttc ccaagaaaga
    43981 tttctactgg tgtagatggt cttggatggg ggatcttacg ttatgagaat tttgatgtat
    44041 cgatccagcc aggtaaaatc ggtgattctt atttaccgtc agagatttat tttgactcag
    44101 gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc
    44161 ataaagaaat cattgatttg gcggtaacag ctgcagaaaa tccaatggca gatgaagcag
    44221 aggattttgc caatgtcatg gctcatccga aagatccagc ttggggattg ttgtatgaag
    44281 agtggatcga gctagctaga aatgtgaacc aagtgattta tgatttaaga aaaaatggcg
    44341 gcatcaaatt cgatgcagat aatgaggaag atttttaatg aacgaatttg tacaagtatt
    44401 tcccgaaaat tggcagaaaa aatatgagga taaagggttt atctcacctt ctcttattca
    44461 acgagcagtt tttcagccac taagtaataa acaaagtata gtagcggttt ctcctacagg
    44521 ttcaggtaaa acactggctt atctttggcc tctactacta aacgtggaac caggtgaagc
    44581 aagtgcgttg gtcatttttg cttcttctca agaactagct attcaagtag cagatgttgc
    44641 acgtgaatgg ggaaaagaca aagagttgaa agtccaatct ttggtgggcg gagccaacgt
    44701 caaacgtcag attgagggtt tgaaggaaaa accagaaatc ttgattggta caccaggaag
    44761 aatattggaa ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa caatggtatt
    44821 tgatgaagca gaccagctat ttgacgctgg gaacagccaa atcattgatc aaattcttca
    44881 tcaagctcca acagagtatc agctagcttt tttttcagcg acagcagatc gttcccttga
    44941 aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag cagaggacga
    45001 ttctcgaaaa ggactgagac attacttttt acgtgtaccg atacgaaaga aagatgacta
    45061 cttgagacgt ctcacgcata tagaagattt tcatggtctg attttcttta atcaactgac
    45121 tgaattaggc gtaatggaag aaaaattgct gtatagaggt gtaccggtag gaagtctggc
    45181 ctctgatcag aacaagctac tccgaaaagc agcgctagaa caattcaaaa agaaaatggt
    45241 cagagcactt ttgaccacag atgtggcggc tagaggttta gatattacag gtcttcctta
    45301 tgtagtgaat gcagaagttc cgttaagtga agaagcttat ttgcatcgtt cgggaagaac
    45361 tggtcgaatg ggtaatgaag gaatagtaat tacactcgta caagataata atctaagaga
    45421 tttaaaacgg cttttacgtc catcaaatat aacacttgaa gagatttttt tatacggagg
    45481 taagctgcaa actgagcagc caaccaaaga agatgcagct cctgcagtaa ataaaaagta
    45541 tgcgaaaact ttttctggaa aggagcagaa cccagaatat aacaaagaaa aaataagaaa
    45601 aaaagttaaa aataaatcaa aaaaagagag aaataaagga gcaaggcgca aataagcgcc
    45661 tttttatgct atgattatga aaacgggaac aaaattggtt gccagaggag gggttatgtg
    45721 aaagaacgtc aagaagagat cttgcagcta ttgacagaaa accaacaagg attgacagca
    45781 agtgatgtag ctgagcgctt aactatcgat cgaagcaatg caagccgata tttaagtgaa
    45841 ctatacaagg cccatcatat tgtaaaaacc gctggccgac cagtcgttta ctctttgcca
    45901 acggaaaagt caaaatctga tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa
    45961 acactcgttg gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt
    46021 ctttatccac cgcgggggtt gcatacgatt atttttggag aaactggtac agggaagtcg
    46081 atgtttgctg aatgtatgta tcattttgca atcgattcag agatgctttc tgcagatgcg
    46141 ccatttgttt ctttcaattg tgcggattat gctcaaaatc cgcaactgct ctttggacac
    46201 atctttggga tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc cggtctaata
    46261 gccaaagcag acggcggtat attgttttta gatgagatcc accgtttacc gccggaaggg
    46321 caggaaatgc tgtttacttt cattgataaa ggtatttacc gtccgcttgg agaaagtgca
    46381 caagtacatg aagcatctgt tcagatcatt ggggcgacga ctgaatcttc agaaagtttc
    46441 ttatctacgt tcaatcgacg gattcctatg gcaatcacct tgccgaatct tgcttcacgt
    46501 tcactggatg agcgttatga aatcatctct ctttttatca aacaagaagc gaatcgtttg
    46561 aatcaacgta tcgatatcga aaaagaagcg attcttgcct tcatgcttta tgatgcggaa
    46621 ggaaatatcg gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc ttttttacat
    46681 taccgtactc atcatgagaa aaagctaatc attcgaaaag aagaactttc ccttcaagta
    46741 caaaaaggat tactgaaaat caaggaaata ccggaacgtc tcgaccgatt catggacagc
    46801 aaaagtcagt atttgacatt tgaaccagga tttgctgatg ttgtctggtc acaagatcca
    46861 gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag tcacttctct ttccgaaacc
    46921 ggtgtggaga acattgatct agagaccctc atttcaaaag atgtggatgc ttatttccag
    46981 acatatgtca aagaattgac taaaagttcg attcccaaag atcttttgcc agatgaaatc
    47041 tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg tatttataat
    47101 gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta cattggatcg tgtgagagaa
    47161 ggtcatatga tcgtccaccc agatttgaat aacgtacgga aaaattttaa gagggaattc
    47221 caggtagcaa tcgatctttc aacgatcatt gaagaagaag aacaagttga gattcctttt
    47281 gatgaaatcg gctttattag tatgtttctt tctatcaatc taggagaaag tgagcctgtt
    47341 aaagaaaaca aagtagatgt tctcatattg atgcacggca gacagactgc ttcaagcatg
    47401 ttggagatgg cacaagaact attaggaaca aaagtaggga aagcttttaa tatgccactg
    47461 gttatggaag ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag
    47521 catttgacga atggcctact cttgttaaca gatatgggat cattaaacac cttcagtgat
    47581 ttgatttatg aagagactgg cattcggacg aaagctatca cgatgacaag taccatgatt
    47641 gtgatagaag cgatacggat ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat
    47701 atccagcttt cctttgaatc gatcgtacgt gatcagttca aagcggaaaa gtctgaaact
    47761 ttacagcgta aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt agctgccaaa
    47821 ttatatcagc gtattatgcc agtgattaat caaaataagg tagaactgat ccagatgcag
    47881 tttttagaac gtgaatcatt caaaaaacac atcgatgcct tattggaaga atatgagatc
    47941 aaagcaattg cgggaacagt tgatgttgat tatcaaaata tcccattttt ttcagcctat
    48001 gacgtttttg atgatgaaaa actaaatatt ttaaagcgta tagctgatga tgagatcccg
    48061 acagctaaaa ttgtcgattc acttgctgga tcattaagcg atgtcttttc tattgatgaa
    48121 ctgatccgtt cattgcaaaa gacagttcag cagatccaga ccgatttgtc gattatcgtg
    48181 gaacctggag tagatactgg aatcgttatc catttagcct tcatgttaga cggattgatc
    48241 aaacaagaaa gccatcggca atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg
    48301 gaaatggata tcgttcgggc aaacttgatg gcactagaac gaaaatatca ggtaaaaatt
    48361 ccagaagatg atatcgcatt tcttacacaa atgtttcttg aaaataaaat aaaaaaatga
    48421 acacactaat catactgttt acacactaaa catctgtgtg cgcactataa aaaagagcta
    48481 aaaagcatga aaacgctatt tcagctcttt tttttattgg catgcttctt gcgttatata
    48541 aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg
    48601 ttcagcaggg atgagtacga gtttattggt cacaaaaatg caaaaagcag cagaagaaaa
    48661 agggatggaa gccgatattt ttgcggtctc tgcctcagat gcagataata atttagaatc
    48721 taagaatgtc gatgtattat tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga
    48781 acaaaaactt gcgcctaaag gtattccatt agatgtcatc aatatgcaag actatggcat
    48841 gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc
    48901 agttgggggt ataacaacgt ggaagatcaa caaaatctag aagccatcat ggggttaatc
    48961 atgtttggcg gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc taaaatcggt
    49021 gattttgagt tagcggatag gaaaatcgct gatgcagaag aatcacttat acaagcgcat
    49081 catgcacaaa cagacatgtt gacacaagaa gcacaaggaa atcatatgca agtgacatta
    49141 ttgactgttc acagtcaaga tcacttaatg acatctattg cgtttactga tttagcaaaa
    49201 gaaatcatcg aactttatcg tcgcgtatac agcgaataaa tacaacttct tttttgaacg
    49261 gaagaagaaa aatttttgaa cggatttttt attttgttat aaggagaact ctctttatat
    49321 aaaaaactta taggaggaag taaaaaatga acggactatc aagttggatg gagaaataca
    49381 tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt agttgctttg cgtgatgctt
    49441 ttatcggcac actgcccgca acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta
    49501 ttcgtgattt accgcaacag tttaacagta cctatgctga cagtttagca gcagacaaag
    49561 gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac
    49621 tagcaatcgc cggattgatc tttgcctttt cttgggggta taatcttgcc cgagcttatg
    49681 gagtaaatga tctagctggg gggatcgttg gtttagctac cttgattcaa ggtatttcct
    49741 ttggtccagc attatcagca gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa
    49801 tcaatagttc tgagattggt gcaacgattg cagatggcac actgtcagtt ggattatggg
    49861 gttggctaaa actagatcat ttgaatggaa acgcttattt tacagttatg atcatgggag
    49921 ctatttcagt cattattttt tgtaaattga tgcttgccaa cattacgatc aaaatgcctg
    49981 attcagtacc accagcagta tctaaagcat ttgcagccat tttaccagcg acgatcgctt
    50041 tatacgtcat tgctattatc aattatatcg taggtcgtgt agctgatggg caactgatca
    50101 ttgatttggt gcaaaaatat attgcagagc cattcctagg attatctcaa gggttgggag
    50161 cagtattgat cgtcacagta tttgttcaag tattctggtt ctttggtatt catggaccaa
    50221 atgtattggc accagtgcta gagggaatct ggggacaagc acaactaatc aatattgata
    50281 ttttccaaaa aggttataac ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag
    50341 atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg tttggcggat
    50401 caggcggaac gatcgtctta ctgatagcta tcttgatttt ctctaaacgt gcagattatc
    50461 taacagtagc taaattatcc attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt
    50521 ttggtctgcc aatcgtattg aatgcaatca tgtttatccc attcttgatt gctccagtag
    50581 tagcaacagc aattggatgg acagctactt atcttggcct agtcgctccg gtatcgcagc
    50641 aagtcacatg ggtagtaccg ccgttcttat tgtcattctt agcaacagga gcggattggc
    50701 gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg
    50761 tcatctctgc aaacaaaatg gaccctgccg caggcgaaca acaataagat gaacgtgagg
    50821 agcgggacgc tttaagtttc gttcctcata atgaattttc gaggaggaac tgaaatgatt
    50881 acaattgcca gtgcaatgca acaagatgaa atcaaagaat tgttagaagg atacaatgaa
    50941 gaaggaatga cttttacatt taaagaaaaa caaggaatca agctgttctt tgaaacaagt
    51001 gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg
    51061 ggaacagtcc tttattttca atcaacagct gtttagaaaa tggtttaaaa agataggatg
    51121 gttgagatga taaaaaacgg tttattttta tgtagtattg gttctttgat cgtgttgtat
    51181 agtttgaatc cacattcgca aaggtatgat ttgtatagta tggtcacagg tctttttcta
    51241 acaggattag gaatctattt cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa
    51301 aataactaat ggaaatcacg aaaaaattaa atattccagc atcgtttttt tacgataaag
    51361 ttatggactc tgtccttttc gatgtacgta aagcgaccgg caaatcagta actagaaaac
    51421 agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa
    51481 tcgaagaagt cattgaaaac cagtcttata agtttcgtac atcgacaacg aagaatgaat
    51541 tcgtcgttca ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt cattacacag
    51601 aaaaaatgga gtcttatggc atgcttcaaa agttgaatga tatgctttta ggaacaatct
    51661 tgatgtattt taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt
    51721 aaatcaatga aacatggtac tatttagtta ttgaaaaatg aagtaagcta gaaggaattt
    51781 ttcataggaa gatagatctt tcctgcgaat cctcactagc ttttttgtag gaaggggcag
    51841 caaaatgaca aacaaagtaa gagagctagg tgtatcttcg attgaggaaa tgtttgattt
    51901 agccacttat gcatttaatg gggcagatac acctgaaagg cgagaacgtt ttcgtacact
    51961 tgcggagaat tcttggaatt acggattttt tgatgaggag gatcggctaa caagtcaagt
    52021 catggcgacc ccttttccag tgaattttta cggacaagag tacctaatgg ccggcattgg
    52081 ctatgttgct tcttatccag aagcaagggg acaaggcggg atcaatcaga tcatggaaaa
    52141 gatccttgaa gattgccgtg atcgtaaagt aagcttatct tatctggcgc cattctccta
    52201 tcccttctat cggcgctatg gctatgaaca atcatttgat aaaatcagtt atcaactaaa
    52261 cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta tggattttga
    52321 agatgcaaaa gctgcaatca gaagaattta tgaacagatg ccggaaaacc aacgagctgg
    52381 tctgaagcgc gaagactggt ggtatacgta taaattcaag caaaaaggca attaccagtt
    52441 tgctttgcat tttgatgata atgaggaagc aacgggatat atcgtttatc agcttgcaac
    52501 gtcttccttt attattgcgg agtggggatt tttggatcat gatgcttttt gcagccttgt
    52561 tcgcttcgta agttctcaca atggtgcttt tgaaacattt gaatattcat gcggctacgg
    52621 cggtaatgat caaaattatc tattagaaaa tccatttgct tctgtaatga tcacacctta
    52681 tatgatggcg aggatcgtag atatttcttt gtttttggaa aattatccat ttaaaaagag
    52741 acaagctgca tttgccctac aaatagaaga agatcattat gcgaaatgga atgaaggaat
    52801 cgtggaagta acaatcgaaa atggaaaaaa tcatctctca aaagtagaac aaacaaaact
    52861 cccaatcgtt cgaggaagta tccaacatat cacgcagcta ttattagggt atcgtaaagt
    52921 ggaagagttg gtattccaag aaagaataca ggtgacaaaa gaatatgaag aaacgttaag
    52981 ctcaatcttg ccacagcaac agccaattct taatgactat ttttgataaa aaacgggtga
    53041 tgacaatatt tttgtcacca cccttttgtt tttcatttgg catttgaagg aaaatgttaa
    53101 agtaaatcct cttttttcgt aaatttgaat tttttgtttt cttttgtttt agggttttct
    53161 tttttgtggt cttggattgc ttctttcgta atcttttccc gatttttttt agatgaatct
    53221 aagcgttctg cgtatacttt ttcttttggc ttatcattca ttggaaagga cctccctaca
    53281 ttatttcatt ctacctctag tttatcatct gttaaggact agtggaaacg ttttgcctaa
    53341 cttataagct gattttttta gcagataagc ttgcaagtaa aaaacaaaca agctacaatt
    53401 gatttattga aattgttcct gtagctcagt aggatagagc aatcgcctcc taagcgatag
    53461 gtcgctggtt caattccagt caggaacgta caaataaccc ctgtatcctt tgcggtacag
    53521 ggggttattt tgtataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
    53581 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc
    53641 catcaataaa cagtcgaaga gaaaataggg aaaaattgat tactaaagaa aaacttacta
    53701 ggtcgacggt gtagttgatt ttcatacttc aatagattcg aattaaacga tttcgattgg
    53761 cttataccag tcctatccaa cgaaagatat atcgttctta actctagcgg tataacttga
    53821 agctttacgt aaagccaatg ggaatgaact ggagatgact ttttattatt tttagtaata
    53881 gctgatgggt taatacttac aactttcctt ttcaaagtac catataaaaa aacggtgggg
    53941 ttgtttagcg aaataaagga tctaagccaa tagcttagat cctttattct atgctttata
    54001 ttattttttg catgtgtgcg aaagtatact caagcccaat cgccatttcg gaaaatcggt
    54061 actgtagagc catcttcacg gattccgtcg atatccatct tgtcagatcc taccatgaaa
    54121 tctacgtggg tttgacttct attcaatcca gcttctgcta attcttcatc ggacatttct
    54181 gtaccgcctt tcacactgaa ggcataagca gaacctagtg ctaagtgatt cgatgcgttt
    54241 tcatcaaata atgtattgaa aaagatgatg ccagattgag aaataggtga agggtcaggt
    54301 acaagtgcga cttctcctaa acgacgcgca ccttcatctg tatcaagcag ttttgctaag
    54361 acatcttctc cttgttcggc agaaaaatca acaacttttc catctttaaa agtaaatttc
    54421 atacccgaga tgatggtgcc agcataactc agtggctttg tacttgaaat gtaaccatct
    54481 actcgatgac tgtcaggtgc tgtgaacact tcttccgtcg gcatatttgc catgaatttt
    54541 tctccacgag cattatagct tccagcgcct tcccaaagat ggtttttagg caatccgata
    54601 atgatatctg taccgggagc agtatagtga agggcagaaa attgttctcg attcaattct
    54661 tctgcttttt ttgctaattt ttcatcatgc ttcttccatg ctaagacagg gtcttcttca
    54721 tagacacgag tcgttttgaa aatctgatcc cataatgcct caacttgttt ttcttctggc
    54781 agttctggga agacttttgc tgcccattgt ttaccagccg cagccacgac agtccaactg
    54841 actttatttg cttgagttgc cttgcgtaaa ttcattagtg ctttgccatt cgctgattga
    54901 taagacgcta ctcgatggct atctacacca gcaaatgcat cgggatcggc agaaacaacg
    54961 ctgatcctgc ttgctccttt ttcaagccat tcatccgctt gatcgatttt agattgtggt
    55021 acattctcaa tacgatccgt tgcagcatgt aaaagaaatt ctctttggat ctggtcgtca
    55081 gtccattgaa cgataacctc agcggctcct agtttataag cttcttgtgt gatcaatcga
    55141 gcaaggggtg cctgttccac gctgatctgt aaaacaactg tgtgtccttt ttctgtggct
    55201 acgccagttt ctgcaattaa gcgtgcatat ttttttagaa gttcattaaa atcagataac
    55261 ataataaaaa ttcctcctca ccattttttc tggtaactat tagcatgata ccatttaatt
    55321 atgattcaaa caatgaggct ttaaataaaa aaaggaggtt atttttttaa ggttaagttt
    55381 catgatatat gttactactt gctatttcag cttgtgaaga aaatcaaaaa ctaattttgc
    55441 atatcaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
    55501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
    55561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
    55621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
    55681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
    55741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
    55801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
    55861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
    55921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
    55981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
    56041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
    56101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
    56161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
    56221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
    56281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
    56341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
    56401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
    56461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
    56521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
    56581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
    56641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
    56701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
    56761 gaaaagaaaa taaaacctaa agacacctgg ggaatgtcat ttggctaaat gtatgccgct
    56821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
    56881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
    56941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
    57001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
    57061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
    57121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
    57181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
    57241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
    57301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
    57361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
    57421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
    57481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
    57541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
    57601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
    57661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
    57721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
    57781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
    57841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
    57901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
    57961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
    58021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
    58081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
    58141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
    58201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
    58261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
    58321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
    58381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
    58441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
    58501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
    58561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
    58621 aataattatg actcttaatt aaagtaggtg cccatatgct tgtttctttt agtatggtta
    58681 taagattttt tctgacaatt ttattatcac tgatattaac acctgtattc aaaatcattt
    58741 cagtacaaac aggtatggtc gacaaaccaa atgaaagaag aataaataaa gttccaatgc
    58801 cttcagcggg cggactgcct atctttattt catttgttat atcgacttta ttcttattcc
    58861 gtaaaattat ccctcaatcg tatattgtac cgatcttgat tgcttcttta gtaatcatag
    58921 tcacaggagt gctcgatgat aaatatgagc tgacacctaa acaaaaaagt atagggatct
    58981 tgattgcttc tcttattatt tattttgtag cagatatacg tattgattcc ttcacgcttc
    59041 cttttattgg atatatccaa ctaggctggc ttagttttcc cgtaacgatt ttttggatct
    59101 tcggtatcac gaatgcggtc aatctgattg acggtttgga tgggctggcg gcagggattt
    59161 cattgatcgg tttaattacg ataggaatca ttggatattt tttcctccat gcttctacag
    59221 tgtatattcc agttgttatt ttctgtcttg tagcaagtat tatcggtttt tttccttaca
    59281 atttttatcc ggcaaagatt tatttaggcg atacaggagc tctattccta gggtttatga
    59341 tggccgtcct ttcactacaa gggctgaaga acgtgacttt tgtttcatcg atttctttgc
    59401 tgatcgtcat gggagtgccg gtaacagaca cgttttttgc tatcattagg agaaaagcaa
    59461 accgtgtttc tttttctaca gcagataaaa aacatcttca tcaccgacta atcagtttag
    59521 gctttactca taaaggtgct gtcctgacga tttatgcgat ggctttgatg ttttctttca
    59581 cagctatggt catgaattat actggaagaa tcggaacgat cgtattgatt attgccatgt
    59641 tgtttgctgc aattttatta tttgaattga ttggtctgat caatgagaaa catcagttta
    59701 ttttaaaaac attgcgattt ttagggaata aagagtaccg tacgcaagta aaacagaaat
    59761 atttgaagaa atggaaccgt catatagatg aataacaaat taaaccagcc gatccttatt
    59821 agtattgtga cggcaaacag tgataaaatt tttcggacac tagataattt tatttcaact
    59881 gtaaaagatg aaacagcagt aaaaatccga atttttgaca acaattcact tcccgagtac
    59941 aaaaaaagac taacagagta taggaaatat ccctacatcg atatctattt tcatgatgaa
    60001 aattccggat ttggttatgg acataatcac aatctgatag ctagcgattc tcgctatggc
    60061 attatttgta acccagatat tttagtggat gaagagacag ttacagcatt ggtcttgtta
    60121 ttgaaagaac atccggaatg tgcgatgtta gcacctaaga ttcttaatga agatggaaca
    60181 actcagcacc tcatccgtga gcagctggct gtttttgatt acgttttgcg ctttgtgcct
    60241 ttcaaatggg tcaaaaaaaa gtttgacaaa cgactggctg tgtttgaatg ccgaggattg
    60301 cctactgatg ctaatagtta tgtacggatg atctcaggta gttttatggt agctgatatt
    60361 gataaattta aagagataaa ggggtttgat gagcgtttct tcatgtactt tgaagacaac
    60421 gacttgtgtt tgagttttga aaaagcaggt tacaagttat tgtacacacc cttttatgca
    60481 gttatccatt tttacgggaa gggtgcgcat cgaaatgcaa aactgtttcg cgtgtttttg
    60541 acttctatga tgaaattttt caataagtgg gggtggcgtt ttttctgatg aaaccagaaa
    60601 tattagtagt cgttgtgctt tatcagcaac tattcagtca atctccagct tatggaccat
    60661 taagcaaagc acttagcgaa aagcaaattc agcttattat ttacgataat agtcctcaaa
    60721 ggcagcaaaa tgagctgttt gaaaaagaaa atatcgttta tcatcatgat ccgcaaaatc
    60781 ctggtttagc ggtggcttat aattttgcgt tgtctcaagc agatgaagga acacgttgtc
    60841 tcgtcactct ggatcaggat acgaggctag tcgacgatta ttttgaacta ttgcgtaaag
    60901 taacctttac agatgaatgt gttgctgcag ttccgatgat tttttctggg agccgacaag
    60961 tttctcccct atattcagat cgttatatca atcgtacagc tcaagcagta gaagttgaag
    61021 gcccaacagc tgaacgaatc atggcaatca attcaggtgt tgcctggtct gtcaaatttc
    61081 taaaaaagat tggcgggttt aatcctgctt tttctttaga ttttttggat cattggctgt
    61141 tttggaaagt ggatcagttg gaaaaaactg tggagatcct tcctgtacga atggaacatg
    61201 atctttcggt tttagactat gaaaaagttt caagtaatcg ataccgttct attttacaag
    61261 cagaaaatcg attttatcat gaatatgatc aaaggcattt atccagtcac cgacgacaat
    61321 tattgttaag aacagctaaa cagttcttga ccgtcaaaaa ccggcatatc tggcggatga
    61381 ctttgcgctc atatgttaag aattggaagg tgtgaattcg tgcgttcggt ttgtatagct
    61441 acctttaatg gaggcagata tttaagagag cagttagata gtattttatc tcagctttca
    61501 gcagaagatg aagtagtcat ttctgatgat gggtcagtag atgatacatg ggatatatta
    61561 actgaatacg cagaaaaaga taagcgaatc cagctttttt cggggcctcg tcaaggattg
    61621 atcgctaatt ttggttatgc gatccagcag acgaaaggag atctaatctt tttggcagac
    61681 caagatgatg tttggttgga gacaaaagtg aaaaagatca ctgactattt tgagcagaat
    61741 ccggatcagc tagtagtagt ttctgattta atcattgtgg acagcgagct gcaagagatc
    61801 catgattcgt atttttcgtt tcgaaattcc aaagaaggat ggcttaataa tgtgataaga
    61861 agtcactata ttggtgctgg tatggctttc cggtcttctt taaaacccga tattttgccg
    61921 atcccgtcag aagtgccgat gcatgatatg tggattggtt tgctgggagg aaagaagact
    61981 gggttcctaa gagaaccgtt aactctgtac cgaagacatg atttaaacgc gagtgagatt
    62041 gaaacacatt ctaaattacg gcaaaaaatt tcttggcgtg ttcatctgat tacggcgttg
    62101 atcaaacgaa aatggtttaa acgttgataa aagatgttta aatccttaat atttccatga
    62161 caaatataac aattgatgag ataataacaa agtgaaattt gaaatcaaaa aggagagcag
    62221 atatccatga agggaatcat tcttgctggg ggcagcggaa cacgtctgta ccctttaaca
    62281 aaggcaacat cgaaacaatt gatgccgatt tatgacaaac caatgattta ttatccaatg
    62341 tctactctga tgttggcggg aatcaaagaa attctgatta tttcaacgcc acaagataca
    62401 ccacgtttca aggaattatt tggaaacgga aatgatttag gactgcagat cgaatatgca
    62461 gtacaagaaa gtccagatgg attggcacaa gcgtttatca ttggagaaga atttattgga
    62521 gatgatagtg tctgcttggt attaggggac aatatttact atggtggcgg tctgtctaaa
    62581 atgttgcaac gagcagcttc aaaagataca ggcgcgacag tatttggcta tcatgtcaat
    62641 gatccagaac gctttggtgt agtagaattt gatgagaata tgcaagcttt gtcgattgaa
    62701 gaaaagcctg ctcagccaaa atcaaattac gctgtaactg gcctttattt ttatgacaat
    62761 gaggtaattt ctatcgctaa aggtatcaaa ccttcagaac gtggagaact agagattacg
    62821 gatgtcaata aagcatattt ggataaagga aaactatccg tagaacttat gggacgcgga
    62881 tttgcttggc ttgatacagg aacacatgaa tcattgcttg aagcatctac ctttatcgaa
    62941 acaattgaaa aacgtcagaa cttaaaagtg gcttgtttgg aagaaattgc ttaccgtatg
    63001 ggctacatcg ataaggaaca attgctggcc cttgcacagc cattgaaaaa aaatcaatac
    63061 ggtcaatatt tattgaattt ggccgctgaa taaggagtcc ttacagatga aagtaacaga
    63121 tacaaaatta acagatgtca aaatcgtaga aatggacgtc tttggcgatc atcgcggttt
    63181 cttcacagaa agttattcaa aagcaaaatt tgcagaacat ggattgaaat atgactttgt
    63241 tcaagacaac cattcactat cagctgaagc tggtgtcctt cgggggcttc atttccaaaa
    63301 aggagaagct gcacagacaa aattgattcg ggtagtcact ggagcagttt tagatgtagt
    63361 agtggatatc cgcaaaggta gcccgactta tggtcaatgg gacggattta tcttatctga
    63421 acataatcat cgacagttgc ttgtacctaa aggatatgcc catggattcg ttactttgac
    63481 accaaatgtg aacttccttt ataagtgtga taattactat aatgcagaag cagatggggg
    63541 catcgcattt gatgatccag atttagcgat tgattggcca attgatccat caagagcgat
    63601 tatgtcggaa aaagatcacc gtcatccgac attaaaagaa tttgaagcag aaaatccatt
    63661 tgtttacgga gaaatctaag aattaaggag aaattttctt atgaaaaaca tcattgttac
    63721 cggaggtgcc ggattcatcg gctctaattt cgttcattac gtggtaaaca atcatccaga
    63781 agtgcatgtc actgttttgg ataaactgac ttatgcagga aacaaagaga acttggccgg
    63841 acttcctagc gatcgcgtag aattggtcgt aggagatgtt gcagatgcag aattagtgga
    63901 ccgtctagta aaagaagcgg acgcagttgt tcattacgcg gcagaatccc ataacgataa
    63961 ttcattaaaa gatcctttcc catttgtaca aacgaatatc attggtacgt atacactttt
    64021 agaagcttgc cgcaagtatg atgtacgcta tcatcacgta tctactgacg aggtttacgg
    64081 cgatcttcca ttacgtgaag acctgccagg acatggggaa ggcgaaggag aaaaattcac
    64141 tgcggaaaca ccatataatc cttcaagtcc atattcttct acaaaagctg gttcagatct
    64201 gttagtaaaa gcatgggtgc gttcttttgg cttacgtgca accatttcaa actgttcaaa
    64261 caactatggt ccttatcagc atattgaaaa atttattcct cgccaagtaa cgagtatctt
    64321 aagcggtatt cgtccaaaat tatatggcga aggaaagaat gttcgtgact ggatccatac
    64381 gaatgatcat tcttcagcag tgtggctgat tttgacgaaa ggcagaattg gcgaaaccta
    64441 tctgatcggt gcagacggtg aagaagataa taagaccgtg atggaattga tcttagaaat
    64501 gatgggccaa ccaaaagacg catacgacca tgtaaacgac cgcgcagggc acgatttgcg
    64561 ttatgcaatc gattcgacaa aattgcgaga agagctaggt tggcagcctg agtttacgaa
    64621 cttccgtgat gggttggctg acacgatcaa atggtacgaa gaacatgaag actggtggaa
    64681 aaaagaaaaa gaggccgttg aagcagcata tgccaaaaat ggacaataat acagaggaaa
    64741 tttcactgta ttaatggaaa aacacatgtg aggcaggcgc tcgcctgtct cttttttaaa
    64801 ataaaaatca aggagcggat aaccatatgt ttttattaac tggaggaaat ggacaattag
    64861 gtacagaatt gcgccattta ttagatgaaa aaaatctgcc atatgtttca acagatgcaa
    64921 aagacttgga tatcacagat gctgagaaaa caatggctta tattacagaa ttaaaacccg
    64981 atgttatttt ccattgcgca gcttacacag cagtagataa agcagaagaa gaagcaaaag
    65041 aattagatga gaaaatcaat gtcgatggca caagaaatgt agcacttgca gcaaaagctg
    65101 tgaatgcaca attcgtctat atcagtacag actatgtttt cgatggaaaa aacaaaaatg
    65161 tagaatacga agtagacgac caaacgaatc cactaaatga atatggtcgt acgaaattat
    65221 taggcgaaaa agcagtagaa gagattttag aagactatta tatcatacga acttcttggg
    65281 ttttcgggat ctatggacac aatttcgtct tcacgatgca acgcctagcg gaaacccgcg
    65341 acaaactgac tgtagtaaat gaccagtttg ggcgaccaac ttggacacgt actttggcag
    65401 aatttatggt atatgtcatc gaagaaaaag caccatttgg tatttatcat ttgtcgaacg
    65461 aaaatagctg ttcttggtat gagttcgcaa aagaaatatt gaaagataca gatgtagaag
    65521 ttgcacctgt tacgtcagaa gaatttccgc aaaaagcaac acgtccgcag tattcagtca
    65581 tgagcctgaa aaagacagaa gcgttaggtt ttgtcatacc aacttggcaa gaagcacttg
    65641 cgcaaatgct ggaagctgcc aaaaaaatga ataagtaatt tcctttttat caaatagaat
    65701 ttggagcatg atatggaaaa acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg
    65761 aatcaggcag attcgaattt ggtgcttgac ctgtttctaa aactccgcta tcttattggc
    65821 gttttagtca tcgtggtcat cgttactttt aatttaaacg gaagttcact tggtgtttgg
    65881 gataaatatg tgtcgcaaag agacgatggg aaaaaaactg atgtgatttt tggacagaat
    65941 cgggaagtgc ggtcagacga atggctggtg cagacacctt tttacctctc acaagctgaa
    66001 gaggactttt cgttggtgaa cgaggattat tcattaaacg gacaaaacat gatcattgcg
    66061 tataattctc ctgtaaagga tattacggtt atcggaaaac cattcaactg gggattcttt
    66121 ttcctcggaa gagatcgagg actttccttt tattgggcga tgaaattagt cgtcatggtc
    66181 ttgcttggat tcgaagtact gatgatatta acgaagagga aaaaagctct atcgctaatc
    66241 ggtgcatttg cgctgacatt ttctccagca gtccagtggt ggttcatgca acatgtgggc
    66301 gatttgattt tctttacttt gggactgatg attgcttttt atcattattt ctacaatcat
    66361 gataaaaaat ggctacgaac gttgatgatg cttttggtgg ttattttcgg tattgggttt
    66421 attttagtga tttacccagc ccatcaagtg atgctggcgt acttgctgat tttttatttt
    66481 gttggcctgc ttatttacta tagcaagaaa gtttcatggg atctgttgga tgtagtgttg
    66541 attggagggg cagttttatt tattggcgga gtcatggttc atttctggct aacgtcaaaa
    66601 gacgcattgt tagcttcttt gaatacctta tatccaggaa aacgggtaag tactggagga
    66661 aagtggacga ttggcgaatt tttttctttc cttacaaatt ggaaaattcc ttttgaagat
    66721 atcaactatt cgaataactc tgaagtggcc ctgttctatc acttttttcc aactgtattc
    66781 ctagcaagtc catttgtgtt atttggcaaa aaagatagcg aacaaaagtt actgggacgt
    66841 attttgatga ttttttgtct gttttctatc ttttggatta ccgttgggtt gccaaaatgg
    66901 ctagcagaaa tcactctgct gtcttatgtt cctccattaa gagccatcct tacttttagt
    66961 tttgcggctt gtttattatc gatctggttt atcgcttata tttggagaga agatgttatt
    67021 cctatttggt ataaattcat tttgttggca gtaaatgcat ggtcttattt ctatgcgatt
    67081 acgcattcga aaatggataa ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg
    67141 ggaacagtga ttttagcttt tgcattattt ggctggagac gcttgtttta cctcgctttt
    67201 gtcgccttag ttatcgtatc tggctttttc gtcaatccag tcgtagaagg tacaggagcg
    67261 atttttgaaa aaacactggc aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa
    67321 gtttggttgt ctgaagatga attatataac ttcaccccaa cattgggagt gaaatccttc
    67381 aatacagtac gcttttatcc agatatagct gcatggaaga aaatcgatcc gaaagaagaa
    67441 tacgagaagt attataatcg gtatgctcat acaagagctt ttatcgctaa ggaagataca
    67501 aagttcattt tagatcgtcc agatatgttt tcggtcacgt tgaattttga agacgcagaa
    67561 aaattaggga tcgactatgt gatcagtaaa cgaccattaa ctgattacaa ccaattatat
    67621 gaagcgcagt tcacgcagct atatggaccg gataaagacg gttatatgat ttttaaagta
    67681 gaatatccgg attatccaga tgtaccacaa acacaaactc aggaaaatca gctagtcgta
    67741 cagtaatagg ctagctgaga ataatcctga aatattagta gaaaggagag tgaagataat
    67801 tcaagcaatc gtacaaaaat ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttgct
    67861 gacgctagtc ttttttgttt ggacagcagg ggtaggtttt ccagcaactt tgtttatata
    67921 taacaatagt tcattactta tcgcttttag tgtggctatt ttgctcattt taaatgttta
    67981 taaagcaaaa ggcgtcgatt ttctttatct aggtgttgcg cttgcccttt tcattttctt
    68041 ttcttacttt cgggaattaa gaaatgagtc tttatttgga gacttactgt ttcctttggt
    68101 tattttattt tgtttagtaa ttaaatggat cgagtggaca aaatgggatc gcggtattta
    68161 tctgattgtc tttacggtct tcctcggaat cacgttatat cgtgtattta cagagatcca
    68221 agtaccagaa ggacaaagca tctgggctcc aggcaattcg ctttctgata tttggatcaa
    68281 tacgaataca atcggaagtt ctttgatgac tcttgcttta ttgatcagtg gatttgccag
    68341 ttcttttgaa cgatggtatg ttcgaatcat cggtgggata gcagtcgttg cagccttttt
    68401 aggtatttgg gtctgccaat ctagaggtgc attagtcgcg ttgattgttt ttgcgatatt
    68461 ggatctttta cctaaatcgt tgatgcgtgt cattcgtgcg ccatttattg cctacacggt
    68521 tacgattctt ttagctttgc cgatttctta tctggcggct gtctctgaaa aagtcaattt
    68581 gtttaccgat cgagaagata tttggcataa attctatcag acgctaggag aaaaatctga
    68641 acaagtttta ctaggaatga aaacatttat cttccagcga ggaaaccaat ttttaggaaa
    68701 ccacaatagt tataattcta ttttgaatat ctatggattg ataggatttg ggattgctgc
    68761 tttgctgctt atccttttta ttggtcgctt aacgttgaaa gcagatttat ccaacgggca
    68821 gatgactttt atctgggcct tttttgcggt gatgatgcag tcatttatgg aggatacatt
    68881 gacttcatac ccatgggttc cgattattta tatcctgttg gctatggccg ttcatcgata
    68941 tgatgaacca gaagttgcgg aggaattcta tgttatggaa gaggcatatg acgatatgac
    69001 ttatgatgat acatcttatg aggaacaacc agatagcgct cattcttcca ggatccaacg
    69061 ctatcattga taggcacaaa atacgagaga ggctgtgaca accgtcgcag ccttttttgg
    69121 tttattgcaa ttgtctagca tcttctgtaa aatagaaacg ttagagaaaa tcttgtatca
    69181 tagaaaatta agaaagaaat aagaacgcag ctcggtacca aaaatatggt aaaatggcac
    69241 ttgactgtaa ttcactgaaa atcgcttgga ggaacacaaa tgtttaaaga tttgtattta
    69301 tttatgaagg acatttatca gaacagaaaa ttattgctac agttttcgat caatgacttc
    69361 aaatcaagat atgcaggatc gtttttagga atcttatggg cgtttatcaa tccgctattt
    69421 actgtattga tttattggct ggtatttggt gttggactga aagcacaaat gatcgatgca
    69481 aaatacccat ttatcgtata tttagttacc gggatgattc cctggttctt cttctcagat
    69541 gcatttgcat caactacggt tgtttttcgg gaatatactt atttagtaaa gaaagtcgta
    69601 ttcaatattc gtatcttgcc cacgacgaaa atcctatcca atctgtacac ccatttattc
    69661 ttcattctga ttggcatagc tgtcgcgctt ataaatggta tatatccttc agcaatgtct
    69721 ttacagctga tttattattt attttgcatg tgtgctttct tgacaggatt gacttggctg
    69781 acggcatcga tccaaccatt tttacctgat atcatgcaat taattacgat cgtgctgcag
    69841 ttgatcatgt ggacattacc gatattatgg agccctaatc agtttgattc aggtcttgta
    69901 agcatattga aattaaatcc gctgtactat gttgttcaag gttatcgtga gtcattttta
    69961 agtgaagctt ggttctggga gcatggaaca tatactttgt atttctgggt tttcacgtta
    70021 gtaatgttcg taatcggctc tgcagtgttc cgtagattga aaccacattt ttcagatgtg
    70081 ctgtaaggag aatagctatg acagaatatg caattagact aaaagatatc acaaaatctt
    70141 ataatatgta cgcaaaacct tcggatcgat ttcgtgaagc cctcaatcct tttaagaaat
    70201 cctatcatga tgtattttat gcgttgaaaa acgtgaatgt agaaataaaa aaaggtgaga
    70261 tgatcggttt cgtgggagaa aacggatcgg gaaaatctac aatgttgaaa atcattacgg
    70321 gagtactgac accaacatcc ggcacgatgg aaatcgaagg aaaaatctca gctttgctag
    70381 aactaggatc aggattcaat cctgagtatt ctggctatga aaatatctat ttaaatggaa
    70441 tggttctagg attttcaaga gaagaagtcg atgaaatggt cgatgatatc attgaatttg
    70501 ctgacatcgg agaccatatt tatcaacctg taaaaactta ttcaagcggg atgttcgttc
    70561 gtctagcttt tgctgtagca atcaatgtga atcccgatat tttgattgtg gatgaagcat
    70621 tggcagtagg cgatcttgaa tttcaactaa aatgtatgga aaaatttaca gaaatcaaga
    70681 actcgggaaa gacgatttta ttcgtttctc atgatatcag ttcgattcgt cgtttttgtg
    70741 atcgttccta ttggttgaaa aacggagaag tcgtcgaata tggcgatacg atggaagtaa
    70801 cgacgaatta tgaaaacttt ttaaagaaaa aatcagttaa aacagtggat cggaataaaa
    70861 accaagtaga acggttcgcc gctccagata tcgtcgacgt gacgaaagcg gaattgttgg
    70921 ataaagacaa aaatccggtg gatatgctgg aacaaggaga aaaactatac gttaaagtaa
    70981 cttatgaagt aaaagatgat acaatcaaaa atccggtttt aggaattgcg ctacgaacag
    71041 tcgacaattt ttatgtatgc ggacttaata ctctactaga tggtatcaag attccttgga
    71101 aaaaaggaga gaatatcttt tatctggagt atgaaaagtt aggtttgtta gctggtgaat
    71161 attattttga cgttgcggtc tttgaagaaa atgcaacggt tcctttggta tataagacaa
    71221 agtacatgaa cttgtttgtc agcggttctt atatcggaga aggcatcgtg gtcttagacc
    71281 ataaatggga agaagggact catagcaatg aaatatgatt ttgaaatgga cctagatgaa
    71341 caaagcagtg tgggaaaaat tgccgcacag atcaaaccag gaagtaaagt tttagagttt
    71401 ggtcccggaa acggccgatt aacgaaacat ttaatcggag caaaacaatg tgaagtgagc
    71461 attgttgagt tagataagga attgttcgat tttgtcagcg aatttgctca agatggattt
    71521 tatggtgata tcgaaagttt tgagtgggca aactattatg ctggacagac atttgattac
    71581 gtcttgtttg cggatgtttt agaacatttg gtagatcctg gaaaaacatt gaagaaagtc
    71641 agagaatttt tgaatgaaga aggagagatc ctgattacat tccctaactt ggcgcataat
    71701 tctgtcatga tcgatttgtt caataaccaa ttgccgtggg cttcttatgg tctcctagat
    71761 gaaacccata attcttttta tactcatgat ggcttccaaa aagtttttga aaaagcggga
    71821 ttattcatta atattgaaga ttatttgtac ttagctgtag gtgatacaga attaaaatca
    71881 acttatgaag aattgccgga agctgtacgc tatgatttca aaatgcgacc atttggtgaa
    71941 gtctaccaat actttttctc attgatgaaa catccagtag cacaaagttc cattgcagag
    72001 ccacaaaatt ctaattatgt aaaagtgtta gaagtaaccc agcaaacgaa acaagatgaa
    72061 acaatccaac aaataccatt caataatttc acaggggaaa atgagacact aagtttcccc
    72121 gtatcagaaa caacagaaag aatggtgttt cgcttcgcgc aacaacctag ctttatcgaa
    72181 ttcagcgcag aagctgccgg agaaaaacta acatttatcg attcgaatgc tgtagtcaaa
    72241 acagtaaatg attgttattt gtttgatggg aaagaattac cagagttcgt tctaacggat
    72301 atttctggga aagaagtgac tatccattgt cactatcgct ttatcgggga attgacacca
    72361 acaatgaaag aattgttgga aacgattcgt cccatggctg aagtcacaaa gcagctttct
    72421 gagaaaaatg acgaattaga aagagaaaat catcatcttc ttgaggaaaa tacaagacta
    72481 gaccatacgt taaaaacaac cacaaatcgc tattgcacat tgttggaaag tgatgaatgg
    72541 acaataaaac atagactagg acgacgtaag aaagaaactt cgaaaaaaat ccaagaaaaa
    72601 gaactttcta tatgtattga cgagaaaatc tgggatgcag aaacaaaaat attaaaaatc
    72661 atcggatggg gaatcgctaa ttcggatcgc cagcccttgt cctataagtt atcagctgac
    72721 caatcgccat ttttcgaagc aattcctgtt tcacgagaag aagtcaatca agcagaacag
    72781 ctcgcaaaag gaacagaagc gggatttgag ttacgaatcc tttgcgagca agaacgttca
    72841 ttcttagtgg aagcagtagc tcaaaatggc caatcatgga tcattgaaat gtaaataatg
    72901 attaacttat aaagataagg agtaaacgaa tcgtgcaaaa aaaagaaatt gccgtattta
    72961 tcgatcaaat cactcgtgaa cgggccactg gcgatttgct tattgtgggc tgggcaattg
    73021 atgaagtgac caaagaaatc cctacgataa aggtagaaaa agaaaatgtg attgctgaag
    73081 caacacatgt cgtaagattg gatatcaatc atttatataa tcttgatgtt aagacccaaa
    73141 gcggtttcaa ggtacgttta tcagggaaaa tgagaggaaa agccatctta gacttccaaa
    73201 cagcgagaca tcaaaatggc atagctgtga aattaaacgg taaataccct tatgatgacg
    73261 gcattgaatc ttcatgggaa agaaaaaaaa gattactgaa aaaagggata aattatgcaa
    73321 ggactcatgg cgtaaaaaaa gcgatccgtc gggtcaaatt ggaattgaag ccaggatcga
    73381 tcgactatgc agagtggatc agtcgtcatg agaaaccaga tttgaaagag caaaggaaag
    73441 aagcacaagg atttgcgtac cgtccattga tttccatact tatgcctgtc tataacgttg
    73501 aaatcaaatg gctcgaaaaa tgcattgatt cggtactgga tcaaacgtat gatcattggg
    73561 aactatgtat cagtgatgat gcttctactg atccagctat ccgaaagtgt ctggaatcct
    73621 atcaagcaaa agatgataga atcaaagtcg ttttccgaaa agaaaatgga cacatcagtc
    73681 tggcaactaa ttctgcattg gaaatagcag aaggagaatt tatagcactc ttagataatg
    73741 atgatgaatt accgccattt gcattatatg aagtggcaaa agtgctaaac gtacatccag
    73801 aactggatgt gatctacagt gacgaagata aaatcgatgc agatggcaat cgctttgatc
    73861 cgcatttcaa agcagactgg tcgcctgata cattgatggg aaataattat atctctcact
    73921 taggcgtata tcgaacaagc attgttaaag aacttggcgg tttccgtaaa ggctatgaag
    73981 gttctcaaga ttatgatttg gtccttcgtg tgacagaaca aatccctgca gatcatattt
    74041 accacattga ccgagtcttg tatcattggc ggacgatccc aggatcaact gcaagtaatg
    74101 gcgaagccaa aagttatatc tatgattccg gagtgaaagc attgacagat gccctgtcta
    74161 gaagaaacat caaaggaagc gtacgtcctg gcagaatttc tggattttat gagattgctt
    74221 atgacgtctt gcaagaggat ttggtcagtg tgatcattcc aacgaagaat ggctatgaag
    74281 atctaaaaac ttgtgtggat tcgatcatcg aaaaaacgtc ttattcaaat tatgaaatca
    74341 ttattgcgga taacggaagt acagatccaa aaatgcaaga attatttgca gaatacaagc
    74401 atcagctgaa agaccgcttt atcgtagaat tgattgacat tccattcaat tattctcgaa
    74461 ttaacaattt agcagcagaa aaagcaaacg gcaaatactt tttattcttg aataacgata
    74521 cagaagtgat tgagccagat tggatgacgg tgatggtttc ttatgcacag tttgatcgaa
    74581 tcggctgtgt aggagcaaaa ctgttttatc cagatgatac aacgcagcat gcaggagtat
    74641 tattaggaat cggtggtgtt gcagggcatg cattgaataa ctatgatcga acacattgtg
    74701 gttatttcgg ccgattggtc attgacgtca attacttagc agtcactgca gcttgtatga
    74761 tggtcaaggc agctgacttc aatgctgtta acgggtttga tgaaacatta gaagtagcat
    74821 tcaacgatgt tgacttgtgc ttgaaagttt atgaactggg tcgttacaat gtctatgcac
    74881 atcaagcaga attgtaccat ttcgaatcta aatcaagagg ttatgaagat actccagaaa
    74941 aacaaagacg ttttgctggt gaaatcaaaa aaatgcaaga taaatggcca gcatatattg
    75001 cgcatgatcc attttacaat gataatttaa caaaagacgg aatcggcgac ttttcgttga
    75061 gaccagatta aactataata aaaagctgtt atgttgtgat tcgctcgcac ataacggctt
    75121 tttatagttt tttgtcaaaa aaaggcagaa ggtgccgact caaaaaaata agtcgaggtt
    75181 gggacagaag tgtttaactc cgagaaataa gaagaaattc acgaaaattg cttttcaaat
    75241 ttttgtgaat ttcggcttat ttaagaagaa gttgcttctg ctcccaccgt ttattcgttt
    75301 ttaaagaatg agacaaaagt gtttcttact tttgtcccac gctcttattc atgctcagag
    75361 cgccaataga tacaaaatca tagccaaagt ttcacgaacc ctagctgtca aagaatcgat
    75421 attttatttt aatataggct attttaaaat aaatggttta aggtattcct ctacaaaagc
    75481 cactctatca attatcatgg gtttaaaata agaagtgatg gagataacca gattgttctt
    75541 agcgtcaata taaagaacat ttccactatt accaattgct gaatatattt cgttctcaga
    75601 atctaatacc caccataaat agccatatct catattttgt aagtcttcta aagcactggt
    75661 tctaggtttg aacatctcgt ttatccacat gggtggtacg atttgttttc ctttatacat
    75721 ccctttattt aggcataata caccaatttt tgccagatca cttgccgata aacacaaacc
    75781 atatcctgcc gtcccgatat ttagtggatc acaaaaccag acattggttt taggagactt
    75841 atccattgta aactgcttat gttcttcagc tgtttcagca taataattga catggtttgg
    75901 tatccctata ggatcaaata gatacttatt ggcaaaatca acagttgtta gcttacttgt
    75961 ctgatacaac aagccagtta atatgtgtaa acagacagtt tgatagttga attcgccggt
    76021 tatacctttt cttcctccca ataaatctag tgatgttttt gtccaatctt cacttgaaca
    76081 gacttttgtc catggatcgc ctttacattt gtagggtgct ttcattgtta gcaagtgctt
    76141 gattgtaata gcgtaaatag ttttctcccc gcgtttcact ttatactctg gaaaataatc
    76201 taatacttta tcatcgatac ttttgattaa tccttgctct aaagcaatgc caatcagcaa
    76261 agatacaata cttttcgttg ctgacatgat atgacaagta tcgtcttttt tataaccatt
    76321 ccattcatct gtataaactt ctaccccatc cttatatgcg acaatttgac atatatttgg
    76381 ttgtcgaaaa ccaataaaat catgcaactc ttgtttattc ataagatatt acctcacttt
    76441 cttctataag cataatgtca ttaaaaatcg atttcaattt tttcgattct tcacgtaata
    76501 caggtattct tatggactag acagcgcttt gggaagatgg attctctgaa aaatataaag
    76561 gttcacttta tgagtggata aaaaaagcag caaatacttc gacttcttta atttaccaac
    76621 attagtgaaa atcgattcat ctggagattt gtaatttaga gtccttgtct tgattagagg
    76681 tgtataaagc tttttattag acatttttaa attccattac aatttgctta taaactaggt
    76741 ttttaaaggt gactatggta agataataga gatattattt tgtgattagg agtgtatata
    76801 tgaataaaaa tggagaatgg agtgctgcac gacggatatt cattatatta atggatgtta
    76861 ttgtatacaa tgcagcaata tatagtagtt tcttactgaa gtttcgtgga gagatcccat
    76921 tacgaaattt agaaactttc caacactcag caattattat ttcagtgatt tttattgcgt
    76981 tgaacatttt gttgggtgca tatgtttttt ataatcgaat gatcagtgac atcgtgtttg
    77041 tcacagtcat cggtcaggta ttgatgacac tagggatcat ggtagtgact tttgcgggcc
    77101 gctggtttgc ttttccacgt cttgttatct tactttcatt tgttctcggt acgcttttct
    77161 taagtatcta tcgtattctc gtatataaac tttatttacg cgtaagtcga gataaaaagg
    77221 tcgtcattat tgggttagaa aaggatgtcg caccagcaat ccaaaatttt ggttcaaaga
    77281 aaaacaacaa acataaagtc gaagcagtca ttcttgataa ctattatgca aacatcaaaa
    77341 atatgattaa agacatcgat attgtctatc ttgcttctca tatcgaagaa actgaaaaaa
    77401 tcaaaatcta tcaattagtt actaaaaatg aaaaaaaatt gtttttgaat acaacatttg
    77461 aaaacttgac attgatcaat ccaaatatta tgaatttcga agatgaaagt attattgaag
    77521 catctggatt tagaatccaa cctgaatatg aactattcaa acgtttgttt gatatcatcg
    77581 tttcacttgt tttattagtt attgcttcac catttatgtt attcacagca attttagtca
    77641 aagtgacatc accgggacca atcatctata aacaagtgcg gatcacaaaa aatcaaaagg
    77701 aattttcaat ctataaattc cgctcgatga ccgccacagc cgaagcaaaa tctggaccag
    77761 ttttagctaa gagcaatgat gcacgagtaa cgcctgtagg taaatttatt cgagctgtcc
    77821 gctttgatga attaccacaa atcttcaatg tgctgaaagg cgatatgtcg atcgtaggac
    77881 cacgtcctga acgttctttc ttcgtcgatc agttcaatgc agaaaaccct tattactact
    77941 tacggcataa tgttcgtgct ggtatcactg ggtatgcaca agtctatgga aaatatgtct
    78001 ctgattacaa cagtaaatta cgttttgatt tactttatat caaaaaatat tcattgatgc
    78061 ttgatttaaa gatcttgctg caaaccatca agatcctatt tgataaaatg tcctctcaag
    78121 gacttgaaga agacgaaaat gtgatctcat tggcagaaat tgcttttccg aatgaaaaaa
    78181 tctttcgcta gttgggaggc gtaaaaatga acaatccgaa attcagcata atcgtaccag
    78241 tatacaatgc agaagcaact attggtcgaa cgattgatat tctaaaagaa ttggattatg
    78301 aagagtacga agtcgtttta gtaaatgatg gatcaacaga tcaaacacag gaaatcattc
    78361 aagaactgat tgaaaatgat gaacgttttg aattgattac tacgaccaat caagggcccg
    78421 gattagcaag aaacgaagga ataagacagt caaaaggaga atatctctta ttcttcgatg
    78481 cagatgacac gccagtaaaa acgatcttgt cagattatga tcatttgcta gaaaagagtc
    78541 caaaagcgga tcttgttgtt agttcgtttg tttttcgtac aatggacgag ggaagaatcg
    78601 tttccgaaaa agaaaactta gttacagaat gtcactatct aactcacgaa gagtttataa
    78661 aagacatgta tcagttaatg aatgaacagt tgatgtatgt tgtatggaac aaatgctatc
    78721 gtagcgatat tctgaaaaat aaccaaattc tatttaaaac ttataatagt tgtgaggatc
    78781 gaatatttaa cttacattat tatgaatact gtcatcaagt gatcatgaac ccaaaagtag
    78841 aatatattta tgaatttgaa ggtgggaagg ggatcactaa tcaataccga cctaacaagt
    78901 tcgaaacatt taaagaattc tattcattag caaatactgt aacagacgat attaataaag
    78961 acggtatggc ggcgcttctt ttaaaaggga caacttcagt tattttttct atttatagta
    79021 cctctgcaag aacttcaaaa gagaagaaag acgaagcaaa gcagatattg agtgatccag
    79081 tgatccgaga agccaagcaa attgctaaga cggattcttc agcaaaaaaa ataacaaagt
    79141 tgctttacaa tatgccagct ccactatttt tatctgctgt aaaagttgga agcttcgttg
    79201 aagtgaaaat gccagggtta atggctgctt tgaagcgagt atattagtta atggaggaaa
    79261 atataatgaa aagaattttg cattttcagg gacgaatggg tttaggtgga gcagagtctt
    79321 tcatgatgaa tttatatcgt aaaattgatc gtacaaacta tcaatttgat tttttgattt
    79381 atgaagatta tgcagatgtt caagactatc attcagagat tgaaagatta ggaggccgta
    79441 tttttgttgt acctaatcct aagaaaaata tttttaaata tttaatagaa gtgaataagt
    79501 tactaaaaaa agaatctttt tctatagttc acaatcaggt ttattttggc gggggaatta
    79561 atttatggtt agccaaaaag aatggaatca gacagagaat tgctcatagc catgcaactg
    79621 aagatggaaa atctcaaaat attgtaatga atgtattgcg taaattttta accaaattgt
    79681 tgttacaaaa tgcaacagac taccttgccg ttagtcaaga agctggtgaa agtctgtttc
    79741 aaaaccatcc ctttgagata gttcataatg gtattgattt agagctttat agtaaaaatt
    79801 cagaagctaa ggttaataag agaaaagaat tggatatctc aatgagtact tttgttgtgg
    79861 gaaacattgg acgattagag aaacaaaaaa atcaatctta tttgttagag atttttttag
    79921 aaatactaaa aatgaattct gattcttact tgattttagt aggtgaaggg tcacttagaa
    79981 ataaactgaa agccaaagca gtagaattgg atatagagaa aagagtaaaa tttttaggag
    80041 aaagaaacga tattcccgag ttattatctg tgatggatgt ttttgtaatg ccttctcttt
    80101 atgaaggtct gccaatggtt ggattagaag cacaagcagc aaaattaaaa ttagtcttag
    80161 cagatactat ttccaaagat acaaagttaa caaataatgt ggattttgta tcattagatg
    80221 aaacaccaca agaatgggca aaaaaagtaa taaaagagcc gtatgctaat aaaataacac
    80281 aagaactaaa gagttatgat gtttcgtaca ctactgaaca aatggagagt atatatagta
    80341 gagatcatta aaaatgttca agatatttta ggagtgatta tttttgaaaa tatgttattt
    80401 gatattggca tacaatgata ttggcaactt aaagagattg gtcaatcgat tgaacgaaaa
    80461 tgcagttttt attattcata tagataaaaa aactgatata gaaccattta ttgaagcatt
    80521 taaatcatat gataatgttg aatttttatg taataaaaac cgtgttaaaa tttattgggg
    80581 tggtttttcc attgtacaag cagaaatcaa tttagtaaag agagcattac aaaatgagaa
    80641 atatttaaaa tatgtattat tatctggtgc tgattatcca atcaaagata atgagtatat
    80701 ttataattat tttaaaaaga atagttc
//
LOCUS       DAZUKR010000003        67616 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000003 DAZUKR010000000
VERSION     DAZUKR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.003"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..519
                     /locus_tag="ACG89C_000173"
     CDS             <1..519
                     /locus_tag="ACG89C_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4898763.1"
                     /translation="DLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYP
                     SIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAI
                     NLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPT
                     QLLDKIEFDYNI"
     gene            719..1033
                     /locus_tag="ACG89C_000174"
     CDS             719..1033
                     /locus_tag="ACG89C_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4898764.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1054..1431
                     /locus_tag="ACG89C_000175"
     CDS             1054..1431
                     /locus_tag="ACG89C_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4898765.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1441..2214
                     /locus_tag="ACG89C_000176"
     CDS             1441..2214
                     /locus_tag="ACG89C_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898766.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2236..3639
                     /locus_tag="ACG89C_000177"
     CDS             2236..3639
                     /locus_tag="ACG89C_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4898767.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3821..5005
                     /gene="mobT"
                     /locus_tag="ACG89C_000178"
     CDS             3821..5005
                     /gene="mobT"
                     /locus_tag="ACG89C_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4898768.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5002..5292
                     /locus_tag="ACG89C_000179"
     CDS             5002..5292
                     /locus_tag="ACG89C_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898769.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5289..5510
                     /locus_tag="ACG89C_000180"
     CDS             5289..5510
                     /locus_tag="ACG89C_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898770.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5552..6331
                     /locus_tag="ACG89C_000181"
     CDS             5552..6331
                     /locus_tag="ACG89C_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS4898771.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6392..6892
                     /locus_tag="ACG89C_000182"
     CDS             6392..6892
                     /locus_tag="ACG89C_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4898772.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6948..7586
                     /locus_tag="ACG89C_000183"
     CDS             6948..7586
                     /locus_tag="ACG89C_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFS4898773.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7658..8053
                     /locus_tag="ACG89C_000184"
     CDS             7658..8053
                     /locus_tag="ACG89C_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4898774.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8037..10490
                     /locus_tag="ACG89C_000185"
     CDS             8037..10490
                     /locus_tag="ACG89C_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4898775.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10487..12514
                     /locus_tag="ACG89C_000186"
     CDS             10487..12514
                     /locus_tag="ACG89C_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4898776.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12511..13533
                     /locus_tag="ACG89C_000187"
     CDS             12511..13533
                     /locus_tag="ACG89C_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS4898777.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13550..14479
                     /locus_tag="ACG89C_000188"
     CDS             13550..14479
                     /locus_tag="ACG89C_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4898778.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14793..14840
                     /locus_tag="ACG89C_000189"
                     /pseudo
     CDS             14793..14840
                     /locus_tag="ACG89C_000189"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14856..15656
                     /locus_tag="ACG89C_000190"
                     /pseudo
     CDS             14856..15656
                     /locus_tag="ACG89C_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            15894..16130
                     /locus_tag="ACG89C_000191"
     CDS             15894..16130
                     /locus_tag="ACG89C_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898779.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16137..18089
                     /locus_tag="ACG89C_000192"
     CDS             16137..18089
                     /locus_tag="ACG89C_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4898780.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18161..18658)
                     /gene="dfrG"
                     /locus_tag="ACG89C_000193"
     CDS             complement(18161..18658)
                     /gene="dfrG"
                     /locus_tag="ACG89C_000193"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4898781.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18933..20060
                     /locus_tag="ACG89C_000194"
                     /pseudo
     CDS             18933..20060
                     /locus_tag="ACG89C_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            20158..20346
                     /locus_tag="ACG89C_000195"
     CDS             20158..20346
                     /locus_tag="ACG89C_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS4898782.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20404..20757)
                     /locus_tag="ACG89C_000196"
     CDS             complement(20404..20757)
                     /locus_tag="ACG89C_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898783.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21288..21710
                     /locus_tag="ACG89C_000197"
     CDS             21288..21710
                     /locus_tag="ACG89C_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4898784.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21707..21937
                     /locus_tag="ACG89C_000198"
     CDS             21707..21937
                     /locus_tag="ACG89C_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898785.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22435..22635
                     /locus_tag="ACG89C_000199"
     CDS             22435..22635
                     /locus_tag="ACG89C_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS4898786.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22662..23855
                     /locus_tag="ACG89C_000200"
     CDS             22662..23855
                     /locus_tag="ACG89C_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4898787.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23923..25488)
                     /gene="guaA"
                     /locus_tag="ACG89C_000201"
     CDS             complement(23923..25488)
                     /gene="guaA"
                     /locus_tag="ACG89C_000201"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4898788.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25547..26470)
                     /gene="coaA"
                     /locus_tag="ACG89C_000202"
     CDS             complement(25547..26470)
                     /gene="coaA"
                     /locus_tag="ACG89C_000202"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4898789.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26666..27271
                     /locus_tag="ACG89C_000203"
     CDS             26666..27271
                     /locus_tag="ACG89C_000203"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4898790.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27579..28595
                     /locus_tag="ACG89C_000204"
     CDS             27579..28595
                     /locus_tag="ACG89C_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4898791.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28736..30037
                     /locus_tag="ACG89C_000205"
     CDS             28736..30037
                     /locus_tag="ACG89C_000205"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4898792.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30083..30745
                     /gene="deoC"
                     /locus_tag="ACG89C_000206"
     CDS             30083..30745
                     /gene="deoC"
                     /locus_tag="ACG89C_000206"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4898793.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30766..31164
                     /locus_tag="ACG89C_000207"
     CDS             30766..31164
                     /locus_tag="ACG89C_000207"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4898794.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31230..32312
                     /locus_tag="ACG89C_000208"
     CDS             31230..32312
                     /locus_tag="ACG89C_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4898795.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32524..34086
                     /locus_tag="ACG89C_000209"
     CDS             32524..34086
                     /locus_tag="ACG89C_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898796.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34079..35212
                     /locus_tag="ACG89C_000210"
     CDS             34079..35212
                     /locus_tag="ACG89C_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898797.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35212..36168
                     /locus_tag="ACG89C_000211"
     CDS             35212..36168
                     /locus_tag="ACG89C_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898798.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36267..37433
                     /gene="deoB"
                     /locus_tag="ACG89C_000212"
     CDS             36267..37433
                     /gene="deoB"
                     /locus_tag="ACG89C_000212"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4898799.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37457..38275
                     /locus_tag="ACG89C_000213"
     CDS             37457..38275
                     /locus_tag="ACG89C_000213"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4898800.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38292..38999
                     /gene="deoD"
                     /locus_tag="ACG89C_000214"
     CDS             38292..38999
                     /gene="deoD"
                     /locus_tag="ACG89C_000214"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4898801.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39297..39983)
                     /gene="gpmA"
                     /locus_tag="ACG89C_000215"
     CDS             complement(39297..39983)
                     /gene="gpmA"
                     /locus_tag="ACG89C_000215"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4898802.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40155..40736)
                     /locus_tag="ACG89C_000216"
     CDS             complement(40155..40736)
                     /locus_tag="ACG89C_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4898803.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40869..41552)
                     /gene="rpiA"
                     /locus_tag="ACG89C_000217"
     CDS             complement(40869..41552)
                     /gene="rpiA"
                     /locus_tag="ACG89C_000217"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4898804.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41871..42284
                     /gene="rpsL"
                     /locus_tag="ACG89C_000218"
     CDS             41871..42284
                     /gene="rpsL"
                     /locus_tag="ACG89C_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4898805.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42368..42838
                     /gene="rpsG"
                     /locus_tag="ACG89C_000219"
     CDS             42368..42838
                     /gene="rpsG"
                     /locus_tag="ACG89C_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4898806.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42932..45019
                     /gene="fusA"
                     /locus_tag="ACG89C_000220"
     CDS             42932..45019
                     /gene="fusA"
                     /locus_tag="ACG89C_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4898807.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45191..46378
                     /gene="tuf"
                     /locus_tag="ACG89C_000221"
     CDS             45191..46378
                     /gene="tuf"
                     /locus_tag="ACG89C_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4898808.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46785..47093
                     /gene="rpsJ"
                     /locus_tag="ACG89C_000222"
     CDS             46785..47093
                     /gene="rpsJ"
                     /locus_tag="ACG89C_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4898809.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47124..47753
                     /gene="rplC"
                     /locus_tag="ACG89C_000223"
     CDS             47124..47753
                     /gene="rplC"
                     /locus_tag="ACG89C_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4898810.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47781..48404
                     /gene="rplD"
                     /locus_tag="ACG89C_000224"
     CDS             47781..48404
                     /gene="rplD"
                     /locus_tag="ACG89C_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4898811.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48404..48694
                     /gene="rplW"
                     /locus_tag="ACG89C_000225"
     CDS             48404..48694
                     /gene="rplW"
                     /locus_tag="ACG89C_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4898812.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48733..49566
                     /gene="rplB"
                     /locus_tag="ACG89C_000226"
     CDS             48733..49566
                     /gene="rplB"
                     /locus_tag="ACG89C_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4898813.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49609..49887
                     /gene="rpsS"
                     /locus_tag="ACG89C_000227"
     CDS             49609..49887
                     /gene="rpsS"
                     /locus_tag="ACG89C_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4898814.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49910..50257
                     /gene="rplV"
                     /locus_tag="ACG89C_000228"
     CDS             49910..50257
                     /gene="rplV"
                     /locus_tag="ACG89C_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4898815.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50271..50927
                     /gene="rpsC"
                     /locus_tag="ACG89C_000229"
     CDS             50271..50927
                     /gene="rpsC"
                     /locus_tag="ACG89C_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4898816.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50930..51364
                     /gene="rplP"
                     /locus_tag="ACG89C_000230"
     CDS             50930..51364
                     /gene="rplP"
                     /locus_tag="ACG89C_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4898817.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51354..51542
                     /gene="rpmC"
                     /locus_tag="ACG89C_000231"
     CDS             51354..51542
                     /gene="rpmC"
                     /locus_tag="ACG89C_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4898818.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51567..51833
                     /gene="rpsQ"
                     /locus_tag="ACG89C_000232"
     CDS             51567..51833
                     /gene="rpsQ"
                     /locus_tag="ACG89C_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4898819.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51891..52259
                     /gene="rplN"
                     /locus_tag="ACG89C_000233"
     CDS             51891..52259
                     /gene="rplN"
                     /locus_tag="ACG89C_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4898820.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52296..52604
                     /gene="rplX"
                     /locus_tag="ACG89C_000234"
     CDS             52296..52604
                     /gene="rplX"
                     /locus_tag="ACG89C_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4898821.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52632..53171
                     /gene="rplE"
                     /locus_tag="ACG89C_000235"
     CDS             52632..53171
                     /gene="rplE"
                     /locus_tag="ACG89C_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4898822.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53190..53375
                     /locus_tag="ACG89C_000236"
     CDS             53190..53375
                     /locus_tag="ACG89C_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS4898823.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53411..53809
                     /gene="rpsH"
                     /locus_tag="ACG89C_000237"
     CDS             53411..53809
                     /gene="rpsH"
                     /locus_tag="ACG89C_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4898824.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53841..54377
                     /gene="rplF"
                     /locus_tag="ACG89C_000238"
     CDS             53841..54377
                     /gene="rplF"
                     /locus_tag="ACG89C_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4898825.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54538..54894
                     /gene="rplR"
                     /locus_tag="ACG89C_000239"
     CDS             54538..54894
                     /gene="rplR"
                     /locus_tag="ACG89C_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4898826.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54915..55415
                     /gene="rpsE"
                     /locus_tag="ACG89C_000240"
     CDS             54915..55415
                     /gene="rpsE"
                     /locus_tag="ACG89C_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4898827.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55430..55609
                     /gene="rpmD"
                     /locus_tag="ACG89C_000241"
     CDS             55430..55609
                     /gene="rpmD"
                     /locus_tag="ACG89C_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4898828.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55653..56093
                     /gene="rplO"
                     /locus_tag="ACG89C_000242"
     CDS             55653..56093
                     /gene="rplO"
                     /locus_tag="ACG89C_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4898829.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56093..57388
                     /gene="secY"
                     /locus_tag="ACG89C_000243"
     CDS             56093..57388
                     /gene="secY"
                     /locus_tag="ACG89C_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4898830.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57450..58097
                     /locus_tag="ACG89C_000244"
     CDS             57450..58097
                     /locus_tag="ACG89C_000244"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4898831.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58288..58506
                     /gene="infA"
                     /locus_tag="ACG89C_000245"
     CDS             58288..58506
                     /gene="infA"
                     /locus_tag="ACG89C_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4898832.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58539..58655
                     /gene="rpmJ"
                     /locus_tag="ACG89C_000246"
     CDS             58539..58655
                     /gene="rpmJ"
                     /locus_tag="ACG89C_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4898833.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58673..59038
                     /gene="rpsM"
                     /locus_tag="ACG89C_000247"
     CDS             58673..59038
                     /gene="rpsM"
                     /locus_tag="ACG89C_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4898834.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59066..59455
                     /gene="rpsK"
                     /locus_tag="ACG89C_000248"
     CDS             59066..59455
                     /gene="rpsK"
                     /locus_tag="ACG89C_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4898835.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59535..60473
                     /locus_tag="ACG89C_000249"
     CDS             59535..60473
                     /locus_tag="ACG89C_000249"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4898836.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60501..60881
                     /gene="rplQ"
                     /locus_tag="ACG89C_000250"
     CDS             60501..60881
                     /gene="rplQ"
                     /locus_tag="ACG89C_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4898837.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61153..61992
                     /locus_tag="ACG89C_000251"
     CDS             61153..61992
                     /locus_tag="ACG89C_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4898838.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            61968..62837
                     /locus_tag="ACG89C_000252"
     CDS             61968..62837
                     /locus_tag="ACG89C_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4898839.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62827..63624
                     /locus_tag="ACG89C_000253"
     CDS             62827..63624
                     /locus_tag="ACG89C_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4898840.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63827..64573
                     /gene="truA"
                     /locus_tag="ACG89C_000254"
     CDS             63827..64573
                     /gene="truA"
                     /locus_tag="ACG89C_000254"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4898841.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64906..67575)
                     /locus_tag="ACG89C_000255"
     CDS             complement(64906..67575)
                     /locus_tag="ACG89C_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4898842.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        22200 a        12061 c        14063 g        19292 t
ORIGIN      
        1 gacttagatt tattacagct aataaacagt gtgaaaagaa tgtatagtgg agacgacaac
       61 ggtgttgatt tatacaaatt tgttaccaaa catatatttg ttaatcttaa tattgacgag
      121 aaaaattacc cgtctatttt taagcactat ctatttttta ttaacgaagt agaagatcgg
      181 ataaaaaata attcattatc tagtagtgta tctagtttta agaagatgtt taaatctcga
      241 gatggtatca cacttactac agcacataaa gttaaaggaa atgagtatga tactgttatc
      301 gctattaatc ttctaaacgg gaaaattcct cattggaatg aaatttttaa tgtttctgat
      361 aaaggaatta gttcagcgag aaaactacta tacgtaattt gttcaagagc aaaaaataat
      421 ttatttcttt tctcagaggt gggttatttt acgagtaaag gagacttgct ttatcccact
      481 caattattag ataaaataga atttgattat aatatttaaa atacctaggt agtcgttgtt
      541 ttaaatatag taggaatcgg acgctcagta cggagcgtct atttttttac ccaattttga
      601 aaggaagtga acttatgaaa ataaaaaatc aagaatcaaa aggtcgctct ccccccttta
      661 aaaccatcac acaaataaga ttcattcaaa cataaaaaca gaaaggatag gtaaaaatat
      721 ggaacttaaa tatgtcattc ccaatatgga aaaaacattt ggacaattag aatatgctgg
      781 tgaagggaac attgaacaac gtcgaataaa tggcagacca accattctct ctcgtagtta
      841 caatttatac tcaacgattc aacgagcaga tgatattgtt gtcattcttc cctccgaagc
      901 tggcgagaaa cattttgaat ccgacgaaaa agtcaaactc atcaacccac atattaccgc
      961 agaaggttat aaaattggca atcgaggatt taccaattac attatgcacg cagacgatat
     1021 ggtgaaagca taagaaagga aaggtgaaaa aatatgagat tagcagaagg tattgtgatt
     1081 gaaaaagacc agacatttgg cacattgaaa ttttccgcct tgcgtcgtga ggttcgtatc
     1141 cagaatgaag atggtacggt ttccgaagaa attaaagaac gtacctatga cttaaaatca
     1201 aaaggacaag gacgcatgat acaagtcagt atccccgctt ctgttccttt gaaagaattt
     1261 gactacaacg cagaagtgga attaatcaat cctgtggcag ataccgtagc gacggctact
     1321 tttcgagggg cagacgttga ttggtacgtg aaagcagacg atcttgtatt aaaaggacag
     1381 acaaaacctc atcagaaata taaaaaagaa gcgtccgaaa gtgaaaaata ggtgttgctt
     1441 atgacaaaca caccattgat tttgaaagaa attccgaagg atgaagccat ttcttttatt
     1501 cggcaatacc attattcaaa gatactccct agattgtgta aatactttct agggattttt
     1561 tcagaagaaa aattattagg cgttgtggaa ttaggctggg gtacgcaacc cctacaaacc
     1621 atacgcaaac tctttcccga cagttccctt caaaccacag attatttaga aattggaaag
     1681 atgtgttttc ttccagaaat gaatcaaacc aattattttg gcagtcaagc tctttcagct
     1741 ctaattaagt ggttgaaaga acatacggac tgtcattttt tatatacact ggcagatgga
     1801 attgagggaa aatgtggcta tgtctatcaa gcgtccaact tcttttactg cgggtatttc
     1861 aagacaagtg tctatcgtga taagcagtca tgggaaaaga tacaccctcg aagtgctcgc
     1921 cttttattag aggaaaatgc tcgctttgaa caggtagaga aaaagcattg gctttcccag
     1981 gcattttgcg aatacaaagg gattgagaaa atcaatggac gcatgtttcg ctatctctat
     2041 ccgttgacaa aagaagcgaa aaaactgtta ggacacactc tctatcgacg tcattattat
     2101 ccgaaagaaa aaaatttacg ctttgaaaag cgaatcgctt atcagaaata tgaagccatt
     2161 tctcaaccaa cttttgataa acaggctcgt atctacaata cacaattatt ttgataggtg
     2221 gaaaggagga tttttatgcc attgcttcat gacagaggag agcgaatcaa agccaacgat
     2281 aagcacttgg tctatcattt tgtactaatt tcattgctgg tagtttttct agtcgtccta
     2341 ctcccttttc attggagagc gttacaatcg gtgaactggc aagcagttca gttagataca
     2401 tttttctatc aacttcatac gccatacttt tggagcagta ttctcactgc tctttttgta
     2461 tgcggactta tcagctggct ttattatcac tatcgtatag attctataaa acagttagag
     2521 caccgtcaaa agttagctcg tatgatttta gaaaatcatt ggtatgaagc cgaagaaact
     2581 caatcagagg gattctttaa agatttaccc agcaagaaaa ccaaacaacg aatccgccat
     2641 tttcctaaaa tatattatcg aatgaaagat ggactaatcc atattcatgt agaaatcacc
     2701 ttagggaaat atcaagatca gcttttgcac ctagaaaaca aactagaaac aggattgtat
     2761 tgtgaattag tggaaaaaac gttgaaagat tcttatgtag agtatgtcct actttatgat
     2821 actattggca atcgaattgg cattgaagac gtggtagctc aagatggacg tgtcaaattg
     2881 atggaatcgg tctattggga gttcgattct ctccctcaca tgttaattgc tgggggcact
     2941 ggtagtggaa aatcctattt tattctcaca ttgattgaat ctctcctgca tacaaatgca
     3001 aagctttata ttcttgaccc taaaaatgct gatttggcag acttaggggc ggtcatgaat
     3061 aatgtctatt accgtaaaga agatatgttg gcatgtatca atcaatttta cgaagatatg
     3121 attgatcgca gtgaaaccat gaaacaacac cctaactata aaacaggaga aaattatgct
     3181 tatcttggtt tgtctgccaa ttttcttatt tttgatgaat atgtggcaat gatggatatg
     3241 ctaggtcgtg aaagtaccac cgttattcat aaactcaaac aaattgtaat gttaggacga
     3301 caagcaggct tctttttaat tcttgcctgt caacgtccag acgcaaaata ttttagtgat
     3361 gggattcggg acaactttaa cttccgtgtg gctttgggtc gtatgtctga aatgggcttt
     3421 ggtatgatgt tcggaagcga tacacaaaaa caatttttct taaaacccat caagggaaga
     3481 ggttacgtgg atacaggaaa aagtgtgatt agtgagtttt atacacccct tgtaccaaaa
     3541 aggtacgatt tcttaggaga aataggaaaa gtcattcaaa aaaagcaatc tgagcctgta
     3601 ccgcacgaag tgaaaggtac aggttcagac ttgctttgat tggtgtggcg ttagccacgc
     3661 caatccttta gccccctcta tctaacaggg gggcacaaat acaactgaaa acaggcaatt
     3721 ctcatcaacc aatgttgatg gtacaaagga ttgcggaaaa tatgaaaggt gtaaactcaa
     3781 gggtcatagt ttacatcttt tttagattgg aggtttgcga atggaaaacg aaaaaatttg
     3841 gattaaggaa ttgaaagaaa agcgactggc ttatggcgtt tctcaaaata aactggcggt
     3901 tgcttctcat attaccagac cttacctatc ggatattgaa acaggcaagg cagtccctac
     3961 tcaacaggtg aaagaagatt tactgaacgc tttggaacgc ttcaatcccg ataacccatt
     4021 ggagatgtta ttcgactatg tacggattcg attccctacc aatgatgtgg ctcaaattat
     4081 cggtgaggtt ttacgtttga atatggatta tatgctacac gaagactttg gctattattc
     4141 ttatcccgaa cactatcgtt tcggtgatat tgttgtccta gtctctcatg atgtaagtaa
     4201 aggtgtttta ttagaactga aaggaaaagg ctgtcggcaa tttgaaaact ttctattggc
     4261 tcaacatcgg agctggtatg atttctttca agattgcatg gaacataaag gtattttcaa
     4321 acgcttggat ttagctatca acgataaaac agggattttg aatattccag atttggcaaa
     4381 gaaatgtaaa caggaagaat gcatttccgt ctttcgaagc tttaaaaatt atcgcagtgg
     4441 tgaattggtt caccgtgatg aaaaaccata catgggaaac acactttata ttggctcatt
     4501 aaaaagtgaa gtctatttct gtatatatga gaaagattat gagcaatacg tgaaaaatga
     4561 tgttccctta gaagacgcag aagtaaagaa tcggtttgaa attcggttaa aaaatgaccg
     4621 agccacccaa gccatgaaag atttattggc acatcaacag gcggaaaaaa cagcctttga
     4681 aattatcaat cgctatatcc gctttgcaga taaagacgac actaaacgtc ggagtgattg
     4741 gaaaactaat gaacgttggg aatggtttat cggtaaaaat cgtggagcat tacgcctaac
     4801 cacacagcca gagccttatt cttttgaacg aacattgaac tggttacatc atcaagtcgc
     4861 ccctacatta aaaatcgcaa gcattttaga tgttctcaac ggtacaacca ttatctccac
     4921 catgattcag gaagccaaac tgacagaaaa acatgagaaa ttaattgaac aacaacatct
     4981 agccatggag gatttgatta catgagcctt ttaacaaaag aattaaagaa gttagggttt
     5041 cagtgtggca ttgagtttca agcgtatata caaaatacag ggaaatatac gtctctcatt
     5101 attgagggaa aacggcaagc tggtgatacc atctatacct atgattttta caaggtgaga
     5161 ttttataata attataccaa tcgagtaacc gtctatggag agcatttaac cccttttcaa
     5221 ttattaaggc gtgtaaaaag ctatatctat tatcgagaaa aatatcttaa ggaaaggagg
     5281 acaatcacat gaattttggg cagaatttgt acaactggtt cttggataat gcacagtcat
     5341 tggtattgtt agcgattgtt gtcattggac tgtttttagg ttttaaacga gaattttcaa
     5401 aactcattgg cttcttgatt attgctctaa tcgccgtcgg acttgtgttc aatgcagctg
     5461 gcgtgaaaga tgtgttatta aacttattta accgaatcat tggtgcttaa cagatatttt
     5521 tcatgataaa ataggaagta tatattttgt tatgaaagga tttatgaata tgtttacaaa
     5581 tgatcaattt aaatctcttt taattaaaac attagaaaga aagagctctt caaattatta
     5641 tgaaggaggt aacgagattg tttctaaaat gaaaatatca gaggtaactt tagatgagac
     5701 tgacgatttt acttattata aaggatataa aaagggctgg aatacgcttt gcacatatct
     5761 taaaataaga gttccttttg atgatttaga cttttttgaa agtcataaag atttgattac
     5821 ccagacagca agctccatat atgataaaca aggtgataat gtattagtgg atactatttt
     5881 agtaccactt ccagagaatt acgaagtaat taactttagt cagttgaaaa ttagtgatgt
     5941 ggtttcacaa gctattgaag acgctgagtc ttttatgagt aatggtgaat atcaacgtgc
     6001 ctttgacaga gttcacactg cttttcatgg atatttaatt gaaatactaa aaaaatatga
     6061 aattacagtt cctagagacg aaaatctatc taaattatat agtaggatac aacaattaat
     6121 tgaaaaagaa attcaaccta ctgaacttgc tgatatagtg aagacaacga taaggtcatc
     6181 aaatggaatg attagctctc ttaatgaagc aagaaatagg cattcacttg cacaccctaa
     6241 tacaaatatt attggaaaga gagaagcaaa actaataata ggtatttcaa gtacagttac
     6301 agattatatt agcggatacc ttgataagta gcgtcattaa ctgattgcta ctttttttat
     6361 gactattttt ataaaaagga tgtgataaga attggaaatg aaagtctatg tggcaaattt
     6421 aggacggtat aatgaaggtg aattagtcgg agcatggttt actccaccaa ttgatgagga
     6481 agaaatggca gaaagaatcg gtttaaatga ggactacgaa gaatatgcaa tacatgattt
     6541 tgagcttcct tttgatgtgg acgaatacac gccgattagt gaaatcaacc gtctatgtga
     6601 agccattcaa gaaatcgaag gtacacctat ttataatgaa ctcaaagaaa ttcaaggcat
     6661 gtggtttagt agcttggaag aattactgga aaacaaagag gatattcatt gttatagtga
     6721 ttgtgattcg atggaagatg tcgcccgtta ttacgtggaa gaaacaggac agcttggtga
     6781 agtcccttcc aatttacaaa attatattga ctaccaatct ttgggacgtg atatggaaat
     6841 agagggtaat tacctagtta cctctcatgg ggtatttgaa tattgtcagt aagtttaaca
     6901 atccgtgtta taattaaatt aataaatagc acggagaggt ataaattatg gtaaatgtat
     6961 ttgaaaatct taaactatta aaactagaca tgaaagataa aggctgggtg atagattcct
     7021 tttactttcg ttacaaacag caaaattata ttgtacttgt taagctattt gaaaaagagg
     7081 aaaaagttcc agaatatgct ctgttaaaac tagaattttt aaaggaaaac gatttttcag
     7141 atatgctagc tgtctatgct aattcggtaa aattatatac agacacgaaa acaataagag
     7201 aatattttgg tatcgaatac agtagtaact tgggagatgt tttatttcaa tttagccaaa
     7261 ctcttgctcg atttattccg acagaagtta gtgagaagaa aaatgaagac caaaaagaag
     7321 ccatgtgttt ctcattaagt caaagtgatt cagaagatcc cagaaaaaaa tattgttttt
     7381 cagttagaag aaatccttta aagggaaatg gtgagctggg aaaacgaagt ccttttaatg
     7441 acaataaaac gagattgtat agacccagct tatatgaacg attagggtca gataccaatt
     7501 taagtttcag atattcaatg aatccagatg atgaagaaac tgatgaaggt attattgcaa
     7561 aatggacaaa aaataaaatt gaatagttaa agcattgaaa gagcaatcac ctattttgat
     7621 tgttcttttt ctatttttac ggaaaggacg tgaagacatg aaaaaaataa gaagttacac
     7681 cagtatttgg tcagtagaaa aagtgattta tgcgattaat gatttacaac tccctttccc
     7741 aattaccttt acgcaaatga catggtttat cgtttctttg tttgtcgtca tactctttgg
     7801 cagtttacca ccactttctc tgattgacgg agcactactc aaatacttag ggattcccat
     7861 tgttctcact gggttcatgt gccaaaagac ctttgacgga aagaaaccct atggatttct
     7921 aaagtctatc attacctatg ctctacgccc taaagtaacc tacgctggca aacaaatacg
     7981 attaaaaaaa gaaacaatga atgaatcgat tacgattgta aggagtgaac aactagatgg
     8041 catatccgat taaatatatg gaagataatc tcgtatttaa tcatgatggc gaagtattcg
     8101 cttattatga attaattccc tataattaca gttttttaag ttccgatgaa aaaattcaag
     8161 ttcatgataa tttctgtcaa ttgattgccc aaaatcgtga tggcaagatt catgctctgc
     8221 aactagcaac cgaatcaagt attcgagcca cacaggaaca atcgaaaaaa gaaatcactg
     8281 gacgattaaa agagatagct tacgaaaaaa tagatgaaca gaccgaagcg ttaatttcta
     8341 tgattggtga caatcaagta gactatcgct tttttattgg ttttaaactc tacctcaatg
     8401 aacaggaagt atccgtaaaa aatgttggga aagaaattca aaaagcattt gcggaattct
     8461 ttcatggtgt gaatcacaaa ttaatgggcg attttgtttc tatgcctatg gcggaaatcg
     8521 aacgcttttc taagatggaa cagttgttag aaagtaaatt atccagacgc tttcagattc
     8581 gtaaattgga gaaagatgat tttggttata ttattgaaca tctttacggg caaacaggtg
     8641 tcccttttga tgactacgac taccatttac cgaaacgata cgaagataaa aaagtgttgg
     8701 taaagaaata cgatattctc aaacccactc gctgtttaat cgaagaaaat caacgctatt
     8761 tacggattga acaggaagat accactactt atgtggcgta ttttacaatt aatagtattg
     8821 ttggtgaatt ggatttccct aatagtgaaa ttttttatta ccagcaacag cagtttactt
     8881 tccccgttga taccagtatg aatgttgaaa ttgtcgccaa taaaaaagcc ttatctaccg
     8941 ttcgcaataa aaagaaagaa ctgaaagatt tagatagcca cgcatgggaa agcgacaacg
     9001 aaaccagtac taatgtggtg gacgctttag aatctgtcga tgaattagaa agtaatttag
     9061 accaaaccaa agaatctatg tacaaactaa gctatgtggt acgggtatca gcaaagaatt
     9121 tggacgagct gaaacaacgt tgtgatgaag taaaagattt ttatgatgat ctgtctatca
     9181 aattagtacg accttttggc gatatgctag gcttgcataa tgaatttatt ccctcaagca
     9241 agcgatatat caatgactat attcaatatg tcacgtcgga ttttttggct ggtcttggtt
     9301 tcggtgctac acaaatgatt ggtgaaaatt ctggtattta cgtcggttac aatctcgata
     9361 caggcagaaa tatttatctc caacctagcc ttgcaagtca aggaattaaa ggctctgtaa
     9421 caaatgcatt agcgtccgct tttattggtt cattaggtgg cggaaaatca ttctctaaca
     9481 atatgctggt ctattattca gtactctttg gcggacaggc aaccattgtt gacccaaaag
     9541 ctgaacgtgg tgaatggaaa gaaaccttac ctgatattgc ccatgaaatc aatattgtta
     9601 atctcacgag tgagaacgac aataaagggt tacttgaccc ttatgtgatt ttaaaactcc
     9661 cgaaagattc agaaagcctt gccattgata ttctcacgtt cttaacaggt atttccagcc
     9721 gagatagcga gaagtttcct gtcctgcgta aagctattcg gagtgtaact caaagcaaac
     9781 aacgagggtt gttattggtg attgatgaat tgcgaaaaga agatacgccc atcagcaata
     9841 gtattgctga ccatatcgaa agctttgtcg attatgattt tgcccatctg ttattcagtg
     9901 atggcagtgt cacccaatcc attagcttgg aaaaacaact caatattatt caagtggcag
     9961 atttggtctt acccgattcc aatagctctt ttgaagaata cactaccatg gaattattaa
    10021 gtgtggcaat gctgattgtc attagtacct ttgctttaga ctttatccac tctgaccgtt
    10081 ccatttttaa aattgttgac cttgatgagg cttggagttt tttacaagtg gcacaaggaa
    10141 aagctctatc tatgcgattg gttcgagcag gacgtgccat gaatgcaggg gtctatttcg
    10201 ttactcaaaa cgcggacgat ttactagatg aaaagatgaa gaataatatt ggcttgaaat
    10261 ttgcctttcg ttccactgac ctagtggaaa tcaaaaagac cttagaattt tttggtgtgg
    10321 acaaggaaga tgaaaacaat cagaaacgct tacgtgaatt agaaaatgga caatgtttag
    10381 tttcagacat ctatggtcgt gtaggggtta tgcagtttca cccgattttt gaagagttgc
    10441 ttcatgcctt tgataccaga ccgcctgtgc gaaatgaggt ggaagaatga cacaaaaaca
    10501 aaagaaattt cttcactata gttggataac gctcctttgt gtgggcgtta ttttactttt
    10561 attaggcacg ctgggcaatg cagtacaagc gacaggctta gttgatgaaa ccattgatac
    10621 aagcaatgaa tactccaagt atggtttgaa tcaatatcag cttgattatt atgtggacaa
    10681 tagctggggc tggcttccat ggaattggag tgatggcata ggacaatcgg tgatgtatgg
    10741 attgtatgcc attactaatt ttatttggac aatttctctt tatctatcga acgccacagg
    10801 ttatttggtg aaagaagcct atgccttaga tttcattagt caaacatccc aagcaattgg
    10861 cgaaaacatg cagacgattg caggcattac accgaatggc ttttccagtt cagggtttta
    10921 tgtcggtttc ctccttcttt ttattctcat tgttggaatc tatgttgcct atacaggatt
    10981 ggttaagcga gaaacgacga aagtgatacg agctgtttta aactttgtcg tggtgtttat
    11041 tctatcggct tcctttattg cgtatgcacc cgattatatt gcgaaaatca atgatttttc
    11101 caaagatgtc agtaccgcca gtctgaatat tgggacaaaa attgttcttc ctaacacgga
    11161 tagtcaaggg aaagacagtg tggacatgat acgtgacagc ttattctctg ttcaagtaaa
    11221 gcaaccatgg ctattgcttc aatatggctc cactgatgta gaagcgttag gggaagaacg
    11281 tgtggaaagt ttattgtcta ccagtccaga tacaaacaat ggtgaagata gagaaaatgt
    11341 agtgattgaa gaaattgaag atcatgacaa tacctattta accctaccga agacgatttc
    11401 cagattagga acagtcttct ttttgtttgt attcaatatt ggtatctcga tttttgtttt
    11461 ccttctaaca ggaatcatga ttttttcgca agtgctattt attattttcg ctatgttttt
    11521 gccaatcagt ttcctattaa gtatgttgcc gagttttgat ggcatgggca ggcatgccat
    11581 taccaagtta tttaatgtga ttatgacaag ggctggtatt accttgatta ttaccgttgc
    11641 ctttagtatt tccactatgc tttattcgct gtcagcaagt tctccctttt tcatgattat
    11701 gtttttacaa attgtgacct ttgcagggat ttattttaaa ctgggcgatt taatgagcct
    11761 attcacttta caaagcaacg actctcaaaa catgggcaga caagtgtttc gcagacctcg
    11821 tcaaatgatg aatcgacaaa ccagaagatt tcaacgcagt atgagtaaga tttttcgtgg
    11881 aaaagcaggg tcaacgaagc aaaagacaat gacaacaaag cagaatcaaa ccaataaagc
    11941 taaccatact cgtcaaaatg aacggactac accaaagaaa gaaacagcac tttccaatgt
    12001 gaaaaagcaa caaggcggta tcgggcaacg attaggtgaa aagacagccc atgtattgga
    12061 cacgaaaaat cgtctagtca ataaaagaaa gcaggcaaaa caacagatac aggcgacacc
    12121 aacccatgtc aaatatgccc ttcatcaagg aaaagagaat gtcactcata atgtaacaga
    12181 ctttaaacaa tctatggcag ataccaaagc tcaacgcaaa caacagcgta tgaaccagca
    12241 ggagcaaaaa cgaaagaaca ttgctcaaaa gcgtttggag ctggataaag caaaagaaaa
    12301 aagacgaaaa gaaaacccat catctcctgt tcagaataac cagcagcatg ttcgcaagcg
    12361 tcagcaaacc gtcacgcctg ttttgtcaca aaacactaaa cgtgaaaatc atcagcgacc
    12421 attgaaaaaa gagcaaaaac aaaagacacc accgaagcaa ttcaatcaga ggaagctaaa
    12481 taaaagaacg gataagcgag gacaacaaac atgaagctaa aaataacagt gatttgtagt
    12541 gtggtcttct ctctcttctt ttttctctta ttattcctag tcattttctt tgcagatgat
    12601 acggacagtg gcgaaaacaa caaggattct tctattccac aaggcggtgt gaccgtttca
    12661 ccagaagtgt tggcacaccg tccgttgatt gaaaaatatg gtaaggaata tgggattgaa
    12721 gactatgttt cttatatctt agccattatg caagtggaat cgggcggtac agcagaagat
    12781 gttatgcaaa gttccgaatc tttgggttta ccgcccaaca gtttgagtac cgaagagtcc
    12841 atcaaacaag gggttaaata tttcagtgaa ttattaacca gtgcagaaca acaaggagtg
    12901 gatatagatt ctgttatcca atcttataat tatggcggtg gctttttaaa ttatgtgagg
    12961 agtcatggga aaaaatatac ctatgaatta gccgaacagt tttctaaaga gaagtcgggt
    13021 ggtcaaaaag cagactatcc caatcctata gccatacctg tgaatggtgg ttggcgatat
    13081 aactatggaa atcagtttta tgttcagttg gtttcacaat acctaaccga cacttcccca
    13141 acagaatttg atgatgaaac cgtgcaagtc attatggacg aagccttgaa atacgaaggg
    13201 tttccctatg tctttggagg ggcgtcgcct accacctctt ttgattgtag tggcttaata
    13261 caatgggtgt atgacaaagc tggcatttct ctccctcgtg tcgcccaaga tcagtatgac
    13321 gctacgcaag aaatctctat ggaagaagcc caagcaggcg accttatctt ctttcattca
    13381 acctataatg caggcacata tgtgactcat gtggcgatat atttagaggg aaatcgcttt
    13441 tatcatgcag gcgacccaat cggttatggc gacttaagca gtcgttattg gcaagaccat
    13501 ctgattgggg ctagacgtgt tattcataac taagaaagga atgaatcaaa tgattataaa
    13561 aatcgagcga aaagaaaaga aggagaaacc aaagaaatct aaaaaaccga gcaagcaaag
    13621 aaaagttccg atgattaaag ttggcacgca taagaaatta acctttgttt tatgggtttt
    13681 attgattggt agtgtcggat ttggaatcta taaaaatttt actgccattg acactcatac
    13741 tgtccgtgaa acagaaatca ttaaacagca gattgtcgat accaaccaag tagaaagttt
    13801 tgtgaaatcc tttgcgaaag actacttttc gtggcaacaa tcccaagaag ccattgataa
    13861 acgaaatgaa aaattgaccc attatttgac ggaagaattg caggtattaa atgaagaaat
    13921 gattcgtaag gatattccta ctagttctag tgtgaacgat atacaggtgt ggcaggtttc
    13981 tcaagtgaat gagaacacct ttgaggtact gttttctgtt gaacaagtta ttaccgaaga
    14041 caaagacaag gaaactatat cgtctagctt tcatgtggtt gtccatatag atgaatcaga
    14101 taatatggtt attatcaaaa atccgacaat gagtaagaaa cctcaaaaat cggactacca
    14161 acccaaacag ctagaaagtg accatacagt agatacagaa acgatggacg aaatcactag
    14221 ctttttagaa acgttcttcc agctctatcc taccgccaca gaaaaagaat tgacttacta
    14281 tgtaagtaat catgtgttac ctatgattaa caaggaatat gttttcgagg aattggtgaa
    14341 tcctatcttt accagaaaag ataaccaagt aatcgtgaat gtcgctgtga aatatttaga
    14401 tcaagaaaca aaggcaaccc aaatctcgca atttgagttg atattagaaa aacaagataa
    14461 ttggaagatt gtaaaataac aaatattggt acatgattac agatactttg taatcatgta
    14521 ctctttttga taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta
    14581 agtgactatt taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa
    14641 tttgatagcg ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc
    14701 cagtttaaga atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt
    14761 gtatacctat ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt
    14821 atgccctttt gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt
    14881 tagctcatgt tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag
    14941 cgattacaga attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag
    15001 aacgtcagag aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga
    15061 aggtgaacat catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat
    15121 tatcagtttt agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa
    15181 ctcgtatatt atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata
    15241 agattgacca aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt
    15301 ctgccgaaat tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact
    15361 ttaccgaatc tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat
    15421 atatgtccgg taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc
    15481 agaattgttc tctgttccct ctttatcatg gaagtgcaaa aagtaatata gggattgata
    15541 accttataga agttattact aataaatttt attcatcaac acatcgaggt ccgtctgaac
    15601 tttgcggaaa tgttttcaaa attgaatata caaaaaaaag acaacgtctt gcatattaag
    15661 tagttcagtt ttggagtaca aaaagtgaaa atgtaaacct aaatgagtat caaattgcaa
    15721 ctgaatttga tactcgtttt ttattgttaa atcaacgttt gttgttaatt tacaccacaa
    15781 aatgtaagtt aaaatgtata taaaaatgcg aacgtgaaat aattattgta ggaatgcaaa
    15841 ttggtgtgtt aaaatggtta caatttacta agcaaaaagg ggtgcatttt cctatgcaat
    15901 ttcttgagcc aaaaaataag aacgctaagt ctgtggattg ggaaatatca gagcaagtaa
    15961 gggtgatagt taagcaatac gctgaatatg cagagcgtac tgaaagcgaa gcagtggatg
    16021 aattcttatt gaacatttta gacgataaga agtttattga atggattgcc aacaagcgaa
    16081 gtaataagcg gattgttgaa aaaatgggta tcaaagatag agtgggttga tagacaatga
    16141 ccaagcttaa aagattagcc acgaaggaag atgaaattgt agatgttaaa attccgattt
    16201 caaacgaaga attaaaagat agggctaaac aatatgattt attaactcca aaacgatttg
    16261 caacaaggta taacaaaatg ttgttcttgc caacgtcttt taaatggaac ggttctgaat
    16321 atcccattca atataattac tgcatcaatc cgttttgttg taattttggg aaagaacagc
    16381 acaaattcaa agatgtaaaa gggaaaccaa gtcgatataa aatgactggt agttccaaag
    16441 ataaaggtca taaaggtatg tactgcaatg acaatcctat tggcagaggt gtatcgcaaa
    16501 actgtacagt aaccccttta tctaattggt ctgttgttga agaaattaaa cgccttatag
    16561 aaatcaatag catccaagat gttgagccag attatcaatt tcataaagaa ggctgctcgg
    16621 aagaagaatc aaccccattc aatgagccga agcaatttta caaaaggggg aaaagtaggg
    16681 gcaaatctca acgctatcaa tgtaaggcgt gtaagaagtt cactaatgtt ctaccaaaaa
    16741 gagaagaaac cactacttat caccaacaaa agaacaccat tcttccaatg ttcgctaaga
    16801 tggttgttgg aagggtttca gtcagtcgta cttgtgatat tttaggaatt ggagttggaa
    16861 cctactatca taagctggag tggctgtata ggcgttgttt ggagttttta gaacgatatg
    16921 agacacaacc ccttcaaaca aagaaattta acgagatgtg gcttaatacg gacaagatgc
    16981 actactacct aaataacgtc cgtaagaaag gacaaggttc aaagaaatat acggggtttg
    17041 aagaccttaa tatgcaaacg tatatagttg tatctgctga ggtcttatca agatatgtgt
    17101 tccgttctga tgttgcctat gattggaata tttcaatgga tgaattaaat gaagatacaa
    17161 gaaaattcaa agaagaccat ctcaatactt ttagtcgtaa aaatgatagg ttagattggt
    17221 cttattatcc acaagaacca tccgctaacg atagcgaaaa taggaatgct tatcttcacg
    17281 aattagggaa aattactaat aggagtagat ttgtcgatgg tttaaatgtt gatgctcctt
    17341 atactacaac tgctcactat tggttaatta aacaaatggt taatgccgac gaatggcgaa
    17401 tgattagtga tgatgacttc tctattagaa acgcctttta tagagtattc acgaaagaat
    17461 taaggttgag cgatgcacat cattttattt gccaagttaa caagacgaaa agcagaaaac
    17521 aatgcttaaa agaattcgga caagcaaaag cagaactgct cgattgggga gatatcagag
    17581 gattcaaaac aaagtttcta cggactttgg catctcatta tctaactgaa ttactaacaa
    17641 gtcatcaatt ccacgaagag gctattagta aagatggaga aagatatcgc aaatatgccg
    17701 ataaccctat caaacatcca ttagccacta aggataaagg attttattca gttgattgta
    17761 gaacggattt atctgctctg gaaccaaacg aaatcgcaaa gatgctatta aatgttaatg
    17821 accattcgac caatagcttt attcaacaga taagacgtta tatatcatct cttgaaagac
    17881 cattaacaac agctcgtggt gataagaaga gctatattta cgctaacttc aatccgaagt
    17941 atgctcaatt tgcgattaca attctaagaa cttactacaa cttttgtcgc ccttttaaat
    18001 cagcggataa aaaggttttg acacctgctc aaagattagg gattacagat aaacaatttg
    18061 attggaaaga tataatttat ttcaaatgac aaaaagagct tggagtaatc ccaagctctt
    18121 gtgctttatc tttactgttc ttcttcaatt aacgcacccg ttaactcaat aagttttttc
    18181 tttcatatac atgaaaatag tagttatacg gatttttatc attctttatc cctttttggg
    18241 caaatacctc attccattcc tcataattca cttctggaaa aaaagtatct ccttcgaatt
    18301 catgatgtat ttttgtgatg tacattttct caacataagg gaaaaacaaa ttataaatct
    18361 gttctcctcc gaaaataaaa atttcttctt cgtttttaca taactcaaaa acatcttcta
    18421 ttgaatgaac aatttcacaa ccattaaagg taaacccctt atctctcgtc agaataatat
    18481 ttcttctgtc aggtaaggct cttccgattg attcaaggtt cttcctacct aatattatcg
    18541 gatgtccctt tgtagtattt ttaacatatt cccagtcctt gggaatcctc caaggaatgt
    18601 cattctcttt gccaatcact ctattcttat ccatcgcagc aatcaaagaa actttcattt
    18661 ataacacagc ctttttaact aatttaccat ctaatttctt atttctatca aaaaaccttt
    18721 tctcattatc ctgcccgtta gttgaataag caattttttc tataagttat attatcataa
    18781 taccacaaaa tcacaaattt tcattcaatt atcattttca attgcatttt ataaatcatt
    18841 ttgtatgtaa tttggtatgt aagttaaaat gaaaaaagta gtccattttg aagtacattt
    18901 tgtcattttg tactccaaaa ctgaactact tacttgcata tatacgcctt tatagtggag
    18961 tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa gttacagaaa
    19021 tgtatacttc aataaatggt gaattatgta agattgatag agcttattct ggagaaattg
    19081 ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc
    19141 cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta
    19201 aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc
    19261 ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag
    19321 tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaactaa
    19381 aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat accattcaca
    19441 tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca ccgcttccgt
    19501 tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat caatcatttc
    19561 aaaatgcagt tatggaaggg atacgctatg gttgcgaaca aggattatat ggttggaatg
    19621 tgacggattg taaaatctgt tttaagtacg gtttatacta tagccctgtt agtactccag
    19681 cagattttcg gatgcttact cctattgtac tggagcaagc ctttagaaaa gctggaacag
    19741 aattgttaga gccatatctt agttttaaag tttatgcacc acaggaatat ctttcacggg
    19801 catataacga tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg aaaaataatg
    19861 aggtcattat tattggagaa attcctgctc gatgtattca agattatcgc aatgatttaa
    19921 ctttttttac aaatgggctt agtgtttgtt tagcagagct aaaaggatat caggttacca
    19981 ctggcgaacc tgtttgccag acccgtcgtc taaatagtcg gatagataaa gtaagatata
    20041 tgttcaataa aataacttag tgcgttttat gttgttatat aaatatggtt tcttattaaa
    20101 taagatgaaa tattctttaa tatagatttg aattaaagtg gaaaggagga gattgttatt
    20161 ataaactaca agtggatatt gtgtcctatt tgtggaaata aaacaagact acgaatacga
    20221 gtggatacta tacttaaaaa tttcccttta tactgcccca aatgtaagaa cgaaacttta
    20281 attaatgttc aaaaaatgaa tataataaca atcaaagagc cagacgccaa gacgcagagc
    20341 cgataatttg agaaatgaaa ctctcatctt atcggctctt tttgtttatc tgaattttac
    20401 tgactagcct tcaatatttc ttgcttcatt gatacctttt gctagtgctt ccattaagga
    20461 tagttctttg tctgtaaagc tatccatgta tttttctacc tgtaaccttc gagtgctttt
    20521 aatcaaatta tctgtaggta agaaaaattc atcaatagat acatggagta atgatacaag
    20581 atcatacagc acttgtgtac ttgggtgttg tcctttgttt tctatatttg ttaaataacg
    20641 tgggtcaatt tcaatcattg ttcccacttg ttcacgagtg agaccccgtt tcattcgagc
    20701 ctctttaatc gctaaaccaa cggctctaaa atcgtattta tcttcttttt tacgcatact
    20761 aaatcacctc tatacatttt actgttccta tataaccata aacaggtata gaaaaacatg
    20821 ttatatagtt tatgagttcc tatagaacac tgtggaattt attaatgcga tgaatccaat
    20881 ttcaaataga aaggtcgctg attttattta cctatacaac tttataaaat ttagtcatac
    20941 tcctagaaag tagataaaat tggcgactta atggagggaa aagtcgccat gagaaagata
    21001 atctgtcatc tagttctctt aattaagttc ttatcaaaaa aacttatcca cctgtactgg
    21061 atagatatga ccatcaatgt ggttttgcag attacataga tagaaaaatc aaagcatatg
    21121 aacagagaaa acaatctgtt tatgtgcttt tttggtcact taataaatat cttttaggat
    21181 ttctcttcat ttatgcaaca aatcccccgt tcgtgtcggg gtttagtatg aagggagata
    21241 aagaagcaca tgccactccc tttcacgaca gaaagggggt gaaaagcatg aaaccatctt
    21301 cttttcaaga aaccatagaa aatcagtttg actatatctg caaacaagtt attgaagatg
    21361 aacgaaaaga ttacttcaaa catctagaca gactatcagc aaaagaagtc tcgttttctg
    21421 atatagataa ctaccttgtc aactcttttt ctactgttga tacgtacgtc actgatttcc
    21481 aactatttaa attatttgga ttaagaatca atgttgaaag tgatttatta agtgaagccc
    21541 tgcgtcatct ttcagagaaa aaacggaaca ttattttact tcattacttt atggatatga
    21601 gtgatgtgga gattgcggag ttactaaatt tgaatcgttc gactgtttat cgtcatcgaa
    21661 taagtggact agccatgatt aaagaattca tgaaggagtg taaagaatga aaacagctta
    21721 tccaagggtt cccttccccc tcattgtaaa ggcgacagat ggcgacgtgg aagcaattaa
    21781 tcagattgtt aaacattatc gagggtatac ctcaaaacgt tcattacgtc gcatgacaga
    21841 cgaatatgga aattctcata tggttattga tgaaacccta cgtggcagaa tggaaacgaa
    21901 actcatctct aagattttat cgttcgaaat taaatagtcc cattttttcg tggaagcgtg
    21961 tcgaatcgta tccacgcttc ccgatttggg aggtttgtta ttccatcaaa gcatattgtg
    22021 tgttcagtag gttttgatag gctaacaagc caaacgttct ttgacaacca aataaaggca
    22081 atcgaatacg tttcggtaag aaaagagctg atgaactaac acgccatgac ctacaaaatt
    22141 agtgagcgat tcatgttagt aattcgaaag taatctttag caggattgct gccatgactt
    22201 tcttatcgtg ataatgatac tcccgtatag ccatagtccg agcgttagaa gcgtcacagg
    22261 caatgggtac ggctacatga gaaccatgca gaagtgaaat tcctgtgagc tttgctagag
    22321 ctgttcgatt gctaagatat tcttctcttg tcagtgtatt attataaaac tatttgatat
    22381 aattataggt gaatattgga gttttcctag cctataattg ggaaaggagt aataatgata
    22441 acggtcacaa aaaaggattt agttgagcta ggatatggaa cttctttttc agcagacatc
    22501 ataaaaaaag caaaagaatt gatgattaca aaagggcata tctactatca atctcgaaag
    22561 ctagatagag tccctcgtga agctgttgaa gaaattctag gaattcggtt ttcaaatgaa
    22621 aacaggtctg agtagttttt gcacagtaag gagtaaaaac tatggttaaa gacccaataa
    22681 aaaaagcaaa gaatggaacc tattattttc gtgcaaatct aggttatgat tctaaaggga
    22741 aaaaaattca aaaatatcgt agtggattca ctactaaaaa agaagcaaga gaagaatatt
    22801 cgaaactgct attgatgaaa ccagaagaat taagtgagaa taaggataaa atgacgttta
    22861 aacattatat ctttgaaatt ttccttccat ggtataagac acaggtaaag ctcagaacat
    22921 atgaaaatcg cttaccaact attaagaagc atttctctta ttttgataaa atggctgttt
    22981 ccgatattga gcctattgat gtgcaaaatt ggcaactaaa attatcaaag aagtgtaagt
    23041 cttcttatgt aagagctgtt caaggactat tttcagttgc tatggatcga gcaattgtcc
    23101 ttgggataac caccacaaat ccatctaaga ttattggcaa tgtgaaaaag caaaagtcta
    23161 aaattgaatt ttggacaaaa gaagaatttg aaaaagtaat ctctctaatt tacaaagaag
    23221 attactatca acacttttta tttatctctc tgtggttttt atttatgact ggaatgcgaa
    23281 ttggtgaagc tactgccatt caatgggaag atattgattt tgactcagga gtactaacca
    23341 tcaacaaaac actctattac aagaatcaat cgaattatag atttgttgaa ccaaaaacaa
    23401 aggcgagcgt tcgtcatatt gtattagata aatgcacatt aacttattta tccgaatgga
    23461 aggaagtaca acaaaatcta attcagacag actttgtaat gagctataac ggtattccta
    23521 cccaaaaaca tacactatca tatgctattg aacgctatgc taagctggct ggtatccatc
    23581 gaatcagaat ccatgggtta cggcattctc atgcttcctt gttaatcagt atgggagaaa
    23641 atccacttat cattaaagat cgtctaggac acgaagatat tgagacgact ttaggaacgt
    23701 atggacatct ttatcctaac agtaatttcg aggtagctaa caaattaaat ggagccattt
    23761 cctttaaaga agcaaaacaa aattatgata cttcaccaaa gaatcaattt actgtagact
    23821 acttgcgaaa caaagaaatg aaaaaagtgc aataaaagtg cagtaaaaag ataagaagag
    23881 ccagaaaccc ttggtataag aggatttcta gctctcctac aactattccc actccactgt
    23941 tgctggtggt ttagaagtga tgtcatacac gatacggttg acgtgggcta cttcgtttac
    24001 gatacgtaca gagatttttt gcaagacttc ccatgggata cgggcaaagt cagcggtcat
    24061 tccgtcgatt gaagttacag cacggatacc gactgtgtag tcgtaagtac gtccgtcacc
    24121 cataacaccg acagagcgga tccctggaag aacagtgaaa tattgccaga tatcacggtc
    24181 aagtccagca gctgcgattt cttcacgcaa gatagcatca gattcgcgta cgatttctag
    24241 cttgtcttca gtgatttcgc caagaacacg aataccaaga ccaggaccag ggaatggctg
    24301 acgccaaacg attgaatcag gcatgcctaa ctgagtccct aattcacgga cttcgtcttt
    24361 gaataatgtg tttaatggtt cgatcaattc aaattgcata tcttctggca gtccacctac
    24421 attatggtga gatttgatcg tctgtgctgt ttctgtacca gattcgataa cgtcagtata
    24481 caacgttcct tgtgctagga aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc
    24541 atcaaaaaca tatacgaatt cattcccaat gattttacgt ttttgttctg ggtcagatac
    24601 acctttcaac ttgtctaaga agcgatctct tgcatcgact ttgatgatgt tcaagccgaa
    24661 ttttccgcct agactttcca ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc
    24721 aacaaagata gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc caacgacact
    24781 agaatcaacg ccacctgaaa gaccaagcaa gacttttttg tcaccaactt gttcacggat
    24841 tttttctact tgcatatcga taaagttatc cattgtccag ttaccttcac agtgacaaac
    24901 atcaaaggca aagtggcgca gcaattcgat tccgtattcg gaatgacgta cttctgcatg
    24961 gaattggata ccataaaaat tacgtgttgt gtcttggata gaagcaatcg gacaatcttt
    25021 gcttgttccg actgtttcaa agccttcagg aactttagtt accaagtcac catggctcat
    25081 ccatactgtt tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc
    25141 taacaaggct ttcccatatt cgcggttttt agcaggctct acggaaccgc caagtttgta
    25201 cgtgatcaat tgcataccat agcaaatccc taatacagga atacctaaat tgaagatctc
    25261 aggatcgatc ccaaatgcat tctcatcata tacactgttt ggtccgccag aaagaatgat
    25321 tccctttgga ttcatcgctt ttacttcttc tgcagtaatg cggtggctca ataattcgga
    25381 aaagactcca agatcacgaa tacgtcttgt gatcaattgg ttatactgac taccgtagtc
    25441 taatacgata atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat ttattacccc
    25501 tatcccttta aattttatct caaaatagtc aaactatttt aaaatgctaa tattttcgca
    25561 tgaaaatcga atcgatgata tgattttcag ttttatggag aattatatcg gctcttcctc
    25621 gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac atttttccat acatcttttg
    25681 ccattttaaa ggcttcttca cgatctccga tagcaaaacg ataataataa ttctctggat
    25741 tttggaaagc cgtatccagt aacgcaccaa aacgatccaa ataccatttt tcgatcagca
    25801 cgggatcggc atcgacatag acagaaaaat caaaaaagtc gctgacgtag atttgctgat
    25861 tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa gatatcaggc tgctggatca
    25921 attcgtactt gtctggaatg atatcataca catcatgtga gtaaacaggt acttttatct
    25981 cttcctgacc agattttaca cggttcaaga aatcgatcag catctccata tcataacttt
    26041 caggaaatcc tttacggtcc atgattccac gctcttttaa tatacgattt ggataaagaa
    26101 atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct
    26161 gcagcagtct ggctgttgta ctttttccaa cggctacact tccggcgatc ccgataataa
    26221 aaggcggtac ttctacgtat ttatgaagaa ataaaccttt actcaagaac aaagattcaa
    26281 attctttcat ataaagataa atcagatgag caagaggtac atagatatct cgaacatcct
    26341 gcatggaaat gcggtcattc aaacttttga tattatctag ttcatcttgt gtcaaaggtg
    26401 cttgcccatt acgataaaat tctcgccatt cttctcttgc cacacggtag tagttcattg
    26461 gatcattcat attagcctcc tgcgttctcc catcaatgga actattttaa cacaaaataa
    26521 acgcaaaccc caccacttaa gagagattgc cgatcttttt gtataatctt tataatagaa
    26581 agaaacggag tcttattctt gacttgcgca cggatacata aaaggataga ctgttgtcat
    26641 gaaagaaatg agaaggatgg aagacatgac aaatcattac tattctgaac gcccagatac
    26701 ggcgcacgat tttgaacaat gggcttttga gttaaaaggc agaacctttc aatttgtaac
    26761 agattctggt gtattttcac gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc
    26821 tttcgattgg gaagagttac ctgatgaagg aaagatctta gatgtgggat gcgggtatgg
    26881 accgattggg ttagctttgg catttgcgac acagcggttt gttgaaatgg tcgacattaa
    26941 tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga
    27001 tatccatcaa tcgaatatct atgaagcagt tcatgaagaa acctatgcag ctatcgtaag
    27061 caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc
    27121 tcggttgaag aagggcggta cactgacaat tgtgatccag aagaaacaag gagcgccgag
    27181 tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa atcgtcacaa aagataaagg
    27241 atattatatc atcaaaagtg tgaaagaata actagctgaa aaatagtgga gcgacgaaag
    27301 agtggaactg ggcgtgcatg tacgggtacg tatcagttct ttttttgttg agaagttttt
    27361 aaaaacaaag aactattttt tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt
    27421 acgataaaat aaataagata cagaacttgt atagaaggct gccaagtcgg tagataaagg
    27481 aggtttttat caaaaaacat actttccttt tttctctatt cttttcttgg gggttttggt
    27541 acactgatag ggaatttaga tagaagggag ccaataggat gaagctgacc atcagagata
    27601 tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa
    27661 gcagatttag tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa catcattata
    27721 agcccgatta tttcgcttca aatgtgatca atcgacattc aaagaccatc ggaatgatcg
    27781 tccctgatgt cactgatttc ttcttctcca aactgatcga aggtgtagag agctatttga
    27841 atcctttggg ctatgtgatc atgttatgca attctcgtca tagccaagaa aatgagatca
    27901 agtatttaca agagctttcc caccgctcag tggacggaat cttgctggcc acaccaaata
    27961 ttttacctga gaaatatgca ttagacagcg gtttttatca aaaaatgcca atcattttaa
    28021 tcgatcgcgg attgaatcgt cgggataacg gacggcttat cgtaaaggaa tatgagggcg
    28081 cctatcaagc tgtttcgctg tttattgaaa atggccatac gaagatcggc atgctgaagg
    28141 aaaccactgg atactatcaa ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag
    28201 atcatggcct gccttttaat gggaaatttg ttgaacatgg agagttgact gtccaaggtg
    28261 gatatactgc atcaaaaaaa ttactcaaac ataaagatat cacggctatt ttttgcggga
    28321 atgatgccat ggcgatcgga tgctatcagg cgatcgatga attgggcaaa aaaatcccag
    28381 aagatatctc agtcatagga ttcgatggat taaagctgtc agaatacatg attccgcaat
    28441 tgacgacagt gcagcagcca agttttgata ttggatttta tgctgcacgt ttcctgatcg
    28501 atacgatcga atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca aaattaatta
    28561 ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg cggtatattg cttatgtaag
    28621 catttacaaa ggctagttag tgcttttttt atttttagta aaccagttta ctaaagtggt
    28681 ttacctttcc taaaaataat gtataatacc gaatgaagac aaggaggatc aggcaatgcg
    28741 aatggtggat ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga
    28801 gtacatcgtc acgaactata cgaatggaaa gattccagat tatcaagtca gtgcattatt
    28861 gatggcaatt ttttatcaag acatgacaaa tgaagaaatc acgaacttaa cattggcgat
    28921 tgctaattct ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg tagataagca
    28981 ttcgacaggt ggcgttggtg acacgaccac attgatttta gcccccctag tagctagtgt
    29041 cggagtgaca gtggctaaaa tgtctggacg cggtctaggt tacacaggtg gtacactaga
    29101 caagctagaa gcaattcccg gatttcagat cgagctttcg gatgaagaat ttgtacgcat
    29161 tgttaatgaa agtaaagtag cggtaattgg tcaatcagga aacttggcac cggcggacaa
    29221 aaaattgtat gccttgcgtg acgtcacagc gaccgttgat tctcttccgc tgattgccag
    29281 ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg atgtgaccac
    29341 tggagacggt gctttcatga agaacatcga agatgcaaga cgtctggcaa agaccatgac
    29401 gagcattggg aaactggcta accgagaaac agtggcggtt atctcagata tgtcagaacc
    29461 tcttggcgaa gctatcggaa atagtctaga agttgtcgag gcaatcgaaa cattacaagg
    29521 aaatggtcca gaagatctag tagaaatgtg ctacgcctta ggaagccaaa tggttgttct
    29581 tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg cttcaagaag ctctggaatc
    29641 aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat caagggggag acccagcgat
    29701 cgtggaacat ccagaacgaa ttttgactgc acgctacaca atggagcttc ctgctaaaca
    29761 aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa tgatgctagg
    29821 tgctgggcgt aaaaccaaag aagaagatat tgatcacgct gttggattga aactgcataa
    29881 aaaaattggt gacactgtga caaaaggtga gtcactattg acgatctata gtaacgacga
    29941 agaaatcact tctgtcattg aattattata taaaaatatt gaaattggtg aatcggcaat
    30001 gaagccaacg ttgattcatg acatcatcac agaataaagg aaaaaagcgg actttaccgt
    30061 tttaaagaaa aggggaaaag aaatggaact aaaccgaatg atcgaccata ctattttgaa
    30121 agcagacgca tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa aataccattt
    30181 ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat tacaaggtga
    30241 gccagtagcc gtatgtacag taatcgggtt tcctctagga gcaaacacac ccgagacaaa
    30301 agcatttgaa acaacagatg ccatcaataa cggtgcagat gaagtcgata tggtcatcaa
    30361 tatcggtgct ttgaaatcaa aggatgatca acaagtccaa aaagatatcg aagcagttgt
    30421 tgaagcagca aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt tattagatag
    30481 agaagaaatc atccgagctt gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac
    30541 atcaactggt ttctcaacag gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac
    30601 tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc cataacgaag aagaagcaat
    30661 ggcaatgatc gaagctggtg ctacaagaat cggaacaagc gcaggagtag caatcgtatc
    30721 tggttcaact ggtgaaggct attaattttt tcaaaggaga agctcatggc agaactcaaa
    30781 caagaatgga tcaacacagc tattgaagcg ttagataaag cctatgttcc ttattcccat
    30841 tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg catcaacatt
    30901 gagaatgcat catttggatt aacgaattgt gccgagcgca cagctttttt caaagcagta
    30961 tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc
    31021 tctccttgtg gtgcatgtcg acaagtaatg gcagaattct gttcccctga tatgccggtt
    31081 acgctagtag gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat
    31141 gcatttactg aaaaagatct ataactcatg ttcattcagt cagctgactg ttgaaaaata
    31201 aaacaattat tttaggaggc tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg
    31261 ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg caacaacggc tctactgatt
    31321 catccggtgg caaaagctca tcagacacaa caactgcagc attgatcaca gacacaggtg
    31381 gtgtagacga ccgttcattc aaccagtcag cttgggaagg actagaaaaa tgggggaaag
    31441 atcaaggact ttcacgagga aatgatggct tccaatattt ccaatcttca aatgaatcag
    31501 attacatccc aaatattgac caagcattga atgctggatt caaaacgatt tttggtattg
    31561 gttataaact aaaaccagcg atccaagaac aagcaacaaa caatacaggt acgaactttg
    31621 ttattatcga tgatgtcatc gatggattag ataacgttgt atctgcaact ttcaaagaca
    31681 acgaagcttc ttatcttgca ggtgttgcag cagcttatac gactgaaaca aacgtagtcg
    31741 gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg
    31801 gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat caatatgctg
    31861 gtgacttctc tgctccagat aaaggacgtt caattgctca aggtatgtat gcacaaaacg
    31921 cagatatcat cttccatgct tcaggcggta caggtaacgg tgtattccaa gaagcaaaat
    31981 cattgaacga atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag
    32041 acgaaggcga atacacatta aacggcgaaa agaaaaactt cacattgaca tctacactga
    32101 aagcagttgg aacagtagta gaagatttgg cacaaaaatc agcggacggt aaattccctg
    32161 gcggcgaaca cactgtttat ggattgaaag aagacggtgt tggtctaaca gaaggacaac
    32221 tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg
    32281 taaaagttcc agaaactcct gaagaaaact aaaaccattt ctatagcctt gttagacaca
    32341 tgtttgacaa ggctatggtt tgattagaac aagaattgtt catttatcca agctgccttg
    32401 tgacagtttg gataagggaa caactccctg aagtaatgtt tgtttcaaaa gaagaacgct
    32461 cactaagcgt gaatcttagt caatcgttgc cctagataga aatcgttagg atgtgaaaaa
    32521 aaggtggttc aagaaaatat tgtcatagaa atgcagaata ttaccaaaga atttggaaca
    32581 ttcaaggcaa atgacaacat caatttacaa gtaaaggcag gagaaattca tgcgttgctt
    32641 ggagaaaacg gtgctggcaa atctacattg atgaacgtgc tttccggatt attagagccg
    32701 acatcaggga aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc
    32761 aaccaattag ggattgggat ggtccatcag cactttatgc ttgttgatgc ctttactgta
    32821 acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa
    32881 gcacgaaaag agatccaaaa agtttctgaa cagtatggat tgtcagtcaa cccagatgct
    32941 tacgttcgtg atatttcagt tggaatggaa caacgggtag aaattttaaa aacactttac
    33001 cgaggagcag atgtactgat ttttgatgag ccgacagctg tattgacccc tcaagaaatt
    33061 gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat cattttgatt
    33121 acgcataagt tagatgaaat caaagcagta gctgaccgct gtacagttat ccgccgtgga
    33181 aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac aacaattagc cgatatgatg
    33241 gttggaagaa cggtttcatt caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt
    33301 ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg
    33361 aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag
    33421 tcggagttga tccaagcttt gactggtttg cgaaaggcag aaagcggaca tatcaagcta
    33481 aaaggggaag acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac
    33541 gtgccagaag accgtcataa atacgggtta gtcctagata tgacattatc tgaaaatatc
    33601 gcactacaaa cctatcatca aaaaccttac agtaaaaacg gcatgctgaa ttattcagta
    33661 atgaatgaac atgccagaga attgatcgaa gaatatgatg ttcgaacaac gaatgaactt
    33721 gttcccgcaa aagctttatc aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc
    33781 gaccgagatc ctgatctgtt gattgttgca aatccaactc gtggactgga tgtaggtgcg
    33841 atcgaattta ttcataaacg tctgatcgaa caaagggaca aatacaaagc agtgttactg
    33901 atcagtttcg aattagaaga aattttaaat gtttcggatc gtattgctgt tatccatgaa
    33961 ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt aggtctattg
    34021 atggcgggct ataccttaga agaagcacga aaagagttag aaaagggggc tggcgcacat
    34081 gaatgatcgc agcgaaaaat tccgtaattt attagtgcct gtactttcag ttgttttagg
    34141 attgatctta ggcgcgatat tgatggctgc ttttggtttt cagcccatcc aaggttattc
    34201 ttctatgcta gatgcagccc ttggttcgca aagagggata ggtgaaacgt taaggcaagc
    34261 aacgccgttg atttttacag cattaggttt ttctgtagct aactcagctg ggtttttcaa
    34321 tattgggctt tctggacaag cactttgcgg gtggatcgtc agtgtctggg tggcacttgc
    34381 gtttccagat ctgccaaaag ttgttttgct tccgttatgc gtgatcttag gtgccttagc
    34441 tggtgcgatg gcagcggctg ttccaggttt acttcgagca ttttttggaa caagtgaagt
    34501 gatcgtaaca atcatgatga actatatctt attatacgta agcacatttg ctttacatga
    34561 agtgatgcct gaaagctatc gctcaagttt ggactcttcc aatatgatca gtcaaaatgc
    34621 ctcactgcgg ttaccatggt taacacaagc ttttggcggc tctcgcgtaa actcaggatt
    34681 atttttagca atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa cgactttagg
    34741 atttgaaatc cgttctgttg gattgaatcc ttttgcttct gagtatgcag gaatgagtag
    34801 taaacgtacg atcgttttgt cgatggtcat ttctggcgct ttggcagggc ttggcggtgt
    34861 ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc tatctattgg
    34921 atttgacgga atggctgttt ccttgcttgg ttctggatca gcaatcggta ttcttttatc
    34981 agcgatcttg ttcagtattt tgaagatcgg tggacttggc atgcagacag gagcaggtgt
    35041 tccgtttgag atcgtcaatg tatcgattgc tttgattatc ttctttgtcg ggatcaactt
    35101 cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc
    35161 gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact
    35221 atcgaattaa ttgccttgat ccttacttct acactggttt attcaacacc gctaattcta
    35281 acgtcactag gcggaacttt ttctgaacga agcgggatcg taaacgtagg gctagaggga
    35341 atcatggtca tgggtgcttt tagttccatc gttttcaact tgacgatgag tccatcactt
    35401 ggttctatga caccatggat cgctatttta gtgggtggcg tcgtaggtat cctattttct
    35461 ctgattcacg cagtagcaac agtcagtttg cgtgctgacc atattatcag cgggacagta
    35521 attaatctga tggcacctgc tttaggtgtt ttcttgatca aagcatggta tggtaaaggt
    35581 caaactgata acatcacgca gaatctgggg tatttttctt tcccaggatt agcagatatt
    35641 ccggtcattg gaccaatttt ctttaaaaat acattcttac ctgcttattg tgctattttg
    35701 attgctgtcc tagcttgggt gatcatcttc aagacgaaat tcggcttgcg cctccgttca
    35761 gttggtgaaa atcctcaagc tgccgacaca ttaggtatca atgtatatgc tatgcgttat
    35821 tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg
    35881 atttccggta acttttcagc tggtacgatc gttggacaag ggttcatctc catggctgct
    35941 atgatctttg gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt ctttggtttt
    36001 gctcaaagct tgagtatcat tggcgcacaa ttaccagtta tttcttcgat tccgccagta
    36061 ttgctgcaaa ttgctccata tttgctgaca atcattgtgc tggttgtttt cttagggaaa
    36121 gcttctggtc caaaagcaaa cgggaagaac tatatcaaat caaaataatc ttttagcgaa
    36181 taaaaagggt gggttacggg aagtgttttt cgtctcgccc tttttgtttt aaaggcaata
    36241 aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt acatctgatc gtcatggatt
    36301 cagtaggaat tggagaagca ccagatgccg ataagttcgg tgatgttggt tctgatacat
    36361 taggacacat tgcgaaagaa gcaggattga acgttccaca tttagagcag cttggtttag
    36421 gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga
    36481 aattagaaga agtttctgta gggaaagata cgatgacagg ccactgggaa atcatgggat
    36541 tgaatatcaa aactccattt cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac
    36601 aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa accttatagc ggaacagctg
    36661 ttattgacga ttacggtgaa caccaaatga aaacaggcga tttaattgta tatacttctg
    36721 ccgatcctgt ccttcagatc gcggcacatg aagaaatcat tcctttagaa gaattatatc
    36781 ggatttgcca attcgttcgt gatataacaa aagatgatcc gtatatgatt ggccgaatca
    36841 ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg aacaagcaat cgccatgatt
    36901 atgcgttgaa tccattcgga cacacagtcc ttgattcttt gaaagaagca ggaaaggacg
    36961 tgattgctgt tggaaagatc aacgatattt tcaacggaca aggaatcact gaatctgtcc
    37021 gcacgaaaag caatatggat ggcgttgacc agctgctgaa agttatgaaa aacgattttt
    37081 caggaatcag ctttaccaat ttagtagatt ttgatgcatt atacggtcat cgtcgggatg
    37141 tagtcggcta tgcgcatgcg attgaagaat ttgacttacg aattccagaa ttgcttgaag
    37201 cgatggaaga agacgacttg ctaatgatta cagccgatca cggaaacgat ccgacgttta
    37261 ctggtaccga tcatacgcga gaatatgtac cgttattggt ttacagtaaa aaaatgaaag
    37321 aacaaggggc attgcctcaa ggatattatt cagatatttc agcaacgatt gctgagaact
    37381 ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa taatcttttt
    37441 aaataggaga aagcaaatga ccaagttaag cgagatgtta caagaaacga cccagtttat
    37501 ccttgataaa ggagtaaaag aaattgattt tggtttgatt ctaggttcag gattaggtga
    37561 attagcagat gaaatcgaag atgctgtaat gatttcttat gatgagatcc cgttctttcc
    37621 tgtttcgaca gtagttgggc atgcaggaaa gctagtttat gggtctctgg caggcaagaa
    37681 agtgttagcc atgcaaggga gattccacta ctatgaaggt cattccatgc aaacagtgac
    37741 ctatccagta cgtgtgatgg ccggactagg ggcccattca gtcattgtga ctaatgcatg
    37801 tggtggtgtc aacgaatcat ttgtaccagg agatttgatg ctgattaccg atcatatcaa
    37861 ttttactggt gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc
    37921 agatatgagt caagcttata cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa
    37981 actaggtttg gatcttaaag aaggcgtata tatgggctat tctggaccaa cttatgaaac
    38041 gcctgcggag atccgaatgt ctcggacaat aggagccgat gcagtaggga tgtcgactgt
    38101 tccagaagtc atcgtagcag ctcacagcgg attaaaagtg ttaggaatct cttgtattac
    38161 caacttagca gcaggtatgc aagcgaatct gagccatgaa gaagttgtag aaacaactca
    38221 gagagtcaaa caatctttta aagcattggt taaagaaaca ttgacattat tgtaatagga
    38281 ggaacaataa aatgagtgtg catattgaag cgaaagaagg cgagatcgcc gataagattt
    38341 tacttccggg agatcctttg cgagctaaat atattgcgga gacttttttg gaagatccag
    38401 tttgttacaa tcaggtacgc ggtatgttag gctttaccgg caaatataaa ggagaacgta
    38461 tttctgttca aggaacagga atgggaatgc catcagccac catctatgcc catgaattga
    38521 tccagtcgta tggtgtaaag aaattgattc gagtaggtac atgcggtgca ttgtcaaaag
    38581 acgtccatgt tcgtgattta gtactagcac aaggtgcagc gaccagttct tcgatgatcg
    38641 aaaaaaattt ccaagccttt cattttcctc caatcagtga tttcaatcta ttactgaaag
    38701 catatgagat agctaaagaa aagggctata cggttcatgt aggaaatgtt ctttcggaag
    38761 attctttcta caaagatgat ttgacagaga cgttccaatt agctgaactt ggcgttttag
    38821 gagtggaaat ggaagcagcg gctctttatt acttaggcgc gaaataccat gtgcagacat
    38881 taagtttgat gaccgttagc gaccatttga tcaccggtga agaaacaaca gctgcagaac
    38941 gtcaatctac gttcaatgaa atgatcgaag taggattgga aacagcgata gcagaataag
    39001 aaggaaatga aaaagctgcc ctgcattaag taataagaac atccaagcaa aaatagctcc
    39061 tcatgttttt gacttggctg gacttattat tgcaaatgta agcttttgaa caccgtttac
    39121 caagaggttg tgaatctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc
    39181 ttttcatgtt tgatgaatat tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt
    39241 atacgaaaaa agggactgtg acaaatcgtg tcacagtccc ttttttatag tgttttttat
    39301 aggtagtatt ttttagcaac tgttaagtca tcatttaatt cataaacaag tggttgacct
    39361 gttggaattt caagatccat gatgtcttcg tcagagatac cttcgatatg tttagctagg
    39421 gcgcgtaatg agttaccatg tgcagctact aaaacagttt tgttgtctag taatgctgga
    39481 gcaatttcat cttgccagaa tggcaaagcg cgttctaatg tcactttcaa gttttctcca
    39541 ccaggaatat cgcgtttgtc taacattgcg taacgacggt catttgctgc agaaccttcg
    39601 tcagtttctt ccattaatgg aggaagagta tcgtaagaac gacgccagat gtgcacttgt
    39661 tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac cttgtaattt accatagtga
    39721 cgttcgttta agcgccatga tttgatttgt ggtacccaaa gttgatctga atattctaag
    39781 atcaagttac atgttttgat tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg
    39841 attccagctt ctttgatttt gcgtccgcct tcttttgctt cttcgattcc ttctggtgct
    39901 agatttacat cagcccaacc agtaaattgg ttcaatgcat tccattcact aagaccgtga
    39961 cgagaaaaaa ctaattttgg catgtttagc tctcctttta agataggttt atatttctga
    40021 aattttcagt tctgttttat tgtacaattt tttggaagag atttccatcg aaaaattaga
    40081 taagattcta tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc cacgcaactg
    40141 ttcgttttta ggtattagat gcataattgc tcgatttctt tttcaggtaa attgatatat
    40201 ttataacatg ttccaactga aacaccgcat tttgaagaaa taaattggat cgtttctttt
    40261 ttttcataat agagacgtcg gatttttttg atcgtttttg catcgatttt tggtcttccg
    40321 ccgatttttc cttttttacg agcttcatct aatccaacta atgtacgttc tttgatcaat
    40381 gcgcactcca tgtttgctag gttttccatt aattggaaat agatttttcc catttcgcgc
    40441 gtatcgattc cttcatcaag actaatcaag tgcagcccgc gatctttgaa taattgcgtg
    40501 agttctgtta attgccgcgt agatttgcct aatcgatgaa gttggcaaat gactagtgca
    40561 tcgccttgtt gcatgtcagc gatcactgac tccaaatgaa cagcagtaga atcattttgt
    40621 tccacttcat atgtctcatg gaaaatttct tgacaaccaa agtcagataa ttgatcaacc
    40681 tgagttgtta aatcaacgtc atttatggtt gtgcgtgcat aaccaataat tttcatggtg
    40741 attcctttct agcagtgatt ttctaactgg atataacagt aaaaatgtag cataaagtac
    40801 gtcaaaaaga tagttttttc gcaaattttc cgctgtcttt ttttattgga tgatcaagca
    40861 gtttttcttt atttacgacg gattgcgtca agaatctcaa cgcctttttc tgagccgaca
    40921 actactttag aaaccatgtt taggaaaagc ccatgttcta ctaccccaac aagttcatct
    40981 aaataggtag ctaagctttc tggctgttcg atgacttcta gatgcagatc aatgatgtaa
    41041 tgaccaccat ctgtggttaa gacttctccg ttttcgtctg tacgcaagac gggttggaat
    41101 cttttttctt caaaaatatg aagtagctgt tgggagccat aagggatcac ttcaactggg
    41161 agtgggaact tcccaagttt atcgactaat ttagaagaat caacgatcca aatcgtttct
    41221 tttgagtacg tagcgactat tttctcgaaa agcaaagcag cgccgccgcc tttgatccct
    41281 tgaaaatcag cactgatttc atcagcaccg tctattgtca aatcgactac ggggacttca
    41341 tcaatactct ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc atttgaagta
    41401 gtaactcctg tgatacgtaa accttcttca cgcatacggc gaccaatctc ttctaccata
    41461 taataggcgg ttgaaccggt cccgagacct acgatcatcc cgtcctttac gaatttcgcg
    41521 gcttcaatgc cagctaattg ttttaaattc atctttgcgt ccaccttttt ttagtttgag
    41581 catcgtgctt tcctcactat tttagcgtga tttacggtaa aaagatacca ttctttttta
    41641 ttccaataaa gctttttcaa aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg
    41701 atattctaac aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct gactgtttat
    41761 caaagcatat ttgataagga tgcagacatt gcatttattt tttatcacaa aaaaagaacc
    41821 acctggatgt gtggaactag acgcaaatcg tgggaaggag gaaacaagga atgcctacaa
    41881 ttaaccaatt agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat tcacctgctt
    41941 tgaacaaagg atataacagc tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac
    42001 gtggggtagc aactcgtgtg ggtacaatga cacctaaaaa accgaactca gctttacgta
    42061 aatatgcacg tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc ccagggattg
    42121 gtcataactt acaagaacac agtgttgtat tattacgcgg tggtcgtgta aaagacttac
    42181 caggggtacg ttaccatatc gttcgcggtg ctttagatac tgctggtgtt aacgaccgta
    42241 aacaaagccg ttctaaatac ggaactaaaa aacctaaagc ttaattttaa ttattaaaca
    42301 aatgaaactt gcttctacta tatagaagta attactatga acaatatttc ggaaggagga
    42361 gttacggatg ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag atcctattta
    42421 taactcaaaa ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat
    42481 tgcagctaac attatctata atgcctttga cttgatcaaa gaatctacag gtaacgatcc
    42541 attagaagtg tttgaacaag caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg
    42601 tcgtgttggg ggttcaaact accaagtacc agttgaagtt cgccctgaac gccgcgttac
    42661 tctaggtctt cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca caatggaaga
    42721 acgcttagca aaagaaatca tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg
    42781 tgaagacact cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga
    42841 ttctctctgt ttggtcggct tttataagtc gacctcagat agtgtcctta tttttagcaa
    42901 ctagatagta aagagaggag aaacaaggag aatgaccaga gaattttctt tacaaaacac
    42961 tcgtaatatc ggtatcatgg cccacatcga tgctggtaaa acgactgcta ctgaacgtat
    43021 cttgtactat actggtaaaa tccataaaat tggtgaaact catgaaggag cttcacaaat
    43081 ggactggatg gaacaagaac aagaacgtgg tattacgatc acttctgctg ctactacagc
    43141 acaatggaag ggtcaccgaa tcaacatcat cgacacacca ggacacgtag acttcacagt
    43201 tgaagttgaa cgttcattgc gtgtacttga tggtgctgta actgttcttg atgcacaatc
    43261 aggtgttgaa cctcaaactg aaacagtttg gcgtcaagca acaacttatg gtgtacctcg
    43321 tatcgtattt gcaaacaaaa tggacaagat cggtgctgac ttcttatatt cagtatcaac
    43381 tttacatgac cgtttgcaag caaacgctca cccaatccaa ttaccaattg gtgcggaaga
    43441 cgacttcaca ggaattatcg accttgtaac aatgaaggct gaaatgtata caaacgatct
    43501 tggaacagag atcgaagaaa cagaaattcc tgaagaatac cgcgaattag ctgaagaatg
    43561 gcgcgaaaaa ttggttgaag ctgtagctga aactgatgaa gaacttactt tgaaatactt
    43621 agaaggtgaa gaaatcactg aagctgagtt aaaagaaggt atccgccgcg caacagttaa
    43681 cgtagaattc tacccagtac tatgtggttc agcatttaag aacaaagggg ttcaattact
    43741 attggacgct gttcttgatt acttgccatc accgttagat atcccagcta tcaaagggat
    43801 tgaccctaaa actgatgaag aagtagaacg tcctgcagac gacagcgctc ctttctcagc
    43861 tttagcattt aaagttatga ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta
    43921 ctctggtgtt ttacaatcag gttcatacgt acaaaacgca acaaaaggca aacgtgaacg
    43981 tgttggtcgt atcctacaga tgcatgccaa ctctcgttca gaaatctctg aagtttacgc
    44041 tggagatatc gctgctgctg ttggtttgaa agacacaact actggggaca cactatgtga
    44101 tgaaaagagt ctagttattc ttgaatccat ggaattccca gaaccagtta tccaagtcgc
    44161 tattgaacct aaatcaaaag ctgaccaaga taaaatgggt gtggctttac aaaaactatc
    44221 tgaagaagat cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc
    44281 tggtatgggt gaacttcact tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt
    44341 tgaagctaac gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg ctggcactca
    44401 agccgaaggt aagtttgtac gtcagtctgg tggtaaaggt caatacggtc acgtatggat
    44461 tgaatttaca ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg
    44521 ggttgttcct cgtgaataca ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa
    44581 tggtgtctta gctggctatc cattagtaga tatcaaagct aaactttacg atggttcata
    44641 ccatgatgtc gattcaaatg aaactgcctt ccgtgttgct gcttctatgg cattacgtgc
    44701 agcagctaag aaagctaatc cagtaatcct tgaaccaatc atggctgtag aagttgttat
    44761 ccctgaagat tatttaggtg atgttatggg tcacgtaact gctcgtcgtg gacgtgttga
    44821 aggtatggaa gctcgcgctg gcggccaaca agttgttcgt gcgatggttc cacttgctga
    44881 aatgtttggt tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta catttacaat
    44941 gacatttgac cactacgaag atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa
    45001 cggcggaaaa gctgaataat ttcttaaaac cggatagtcc tgcaaagaga ttatccaaag
    45061 agactatatt tttaatatag tttcgtgtaa aataattatc gatgatatgg gcatgtggtg
    45121 taccttgtgt acatcatata cctatcagac aaataaaaaa tatattgtat ttttaggagg
    45181 acattttaaa atggcaaaag aaaaatttga ccgttctaaa ccacacgtta acattggtac
    45241 tatcggacac gttgaccatg gtaaaactac attaacagct gcaatcacaa ctgtactatc
    45301 taagaaaaac ggcggccaag ctatggctta cgatcaaatc gatggtgctc cagaagaacg
    45361 cgaacgcgga atcacaatct caacagctca cgttgagtat gaaacagata cacgccacta
    45421 cgctcacgtt gactgcccag gacacgcgga ttacgttaaa aacatgatca ctggtgctgc
    45481 tcaaatggac ggagctatct tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg
    45541 tgaacacatc ctattgtctc gtcaagttgg tgttccttac atcgttgtat tcttgaacaa
    45601 agtagacatg gttgatgacg aagaattact agaattagtt gaaatggaag ttcgtgacct
    45661 attaacagaa tacgaattcc ctggtgacga tgttcctgta gttgctggat cagctttgaa
    45721 agctctagaa ggcgacgctt catacgaaga aaaaattctt gaattaatgg ctgcagttga
    45781 cgaatacatc ccaactccag aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga
    45841 cgtgttctca attactggac gtggtactgt tgctacaggt cgtgttgaac gtggacaagt
    45901 tcgcgttggt gacgaagttg aagttgttgg tattgctgaa gaaacttcaa aaacaacagt
    45961 tactggtgtt gaaatgttcc gtaaattgtt agactacgct gaagctggag acaacattgg
    46021 tgctttacta cgtggtgttg cacgtgaaga catccaacgt ggacaagttt tagctaaacc
    46081 aggtacaatc acacctcata caaaattctc tgcagaagta tacgtgttga caaaagaaga
    46141 aggtggacgt catactccat tcttcactaa ctaccgtcca caattctact tccgtacaac
    46201 tgacgtaaca ggtgttgttg aattaccaga aggaactgaa atggttatgc ctggcgacaa
    46261 cgtaacaatg gaagttgaat taatccaccc aatcgctatc gaaaacggta ctaaattctc
    46321 aatccgtgaa ggcggacgta cagttggtgc cggtgtcgtt acagaaatca aagcttaatt
    46381 cacttaagtt ttaaagtaaa gcaacattta ttcggacgta atcgctcgga acgaaggtct
    46441 taggatcttt gttccgagct tttttattta ttgcaggatc aggaaaaaag acaggagtat
    46501 tataaataga agaaacacga aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa
    46561 acaaaatgtt ttttacaaga aaaagccttg ctttgtgagt ttatatatat tataatgaca
    46621 aaggtgcgtt tactttagtg aattgcacag acgtaatcag aatgatttcg tcggctaaga
    46681 aacgagaggt tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg gaaattttcg
    46741 tggagctatg tctacttttc aaaataggcg aaggagggaa caagatggca aaacaaaaaa
    46801 ttcgtatccg tttaaaagcg tatgaacacc gtattttaga tcaatcagcg gataaaatcg
    46861 tggaaactgc aaaaagaact ggagctagcg tgtctggtcc aattccatta ccaactgaac
    46921 gcagtcttta cacaatcatc cgtgcgactc ataaatacaa agattcacgc gaacaattcg
    46981 aaatgcgtac acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg
    47041 ctttgatgaa gctagactta ccatctggtg taaacatcga aatcaaatta taaaactaaa
    47101 acaaaaataa tggaggtgta ctcatgacca aaggaatctt agggaaaaaa gtgggaatga
    47161 cacaaatctt cactgaatct ggtgaattaa ttccagtaac tgttgtagaa gctactccaa
    47221 acgtagtact acaagtgaaa acaatggaaa cggatggcta cgaagctatt caagttggtt
    47281 accaagatat gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa
    47341 acacggctcc taagcgcttc attcgtgaat tcaaaaatgt tgagctagga gaatatgaag
    47401 taggaaaaga aattacagta gatgttttcc aagcaggaga cattattgat gtaacaggta
    47461 ctacgaaagg taaaggattc caaggggtta tcaaacgcca cggacaaagc cgcggaccaa
    47521 tggctcacgg ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc
    47581 gtgtatttaa aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta actatccaaa
    47641 acttggaagt tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc
    47701 ctggagctaa aaaatcttta attactatca aatcagctgt gaaagctaaa taattgtagg
    47761 aagaaaggag gaactaagga atgccgaatg tagcattatt caaacaagat ggaagccaaa
    47821 acggtgaaat cacacttaac gaagaaatct tcggaattga acctaatgaa agtgttgttt
    47881 acgatgcaat cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc
    47941 gtagcgctgt tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac
    48001 gtcaaggatc tatccgctca ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac
    48061 cacgttctta cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat
    48121 tgtcagaaaa agttgctgaa aacaacttgg tagcaatcga aggcttgaac ttcgacgcac
    48181 caaaaacaaa agaattcaaa caagttcttg ctaacttatc tatcgactct aaagtattgg
    48241 ttgttctaga aaaaggtaac gactttgctg ctttatcagc acgtaactta ccaaacgttt
    48301 ctgtagtaac ttctgataat gtgagtgtgc tagacgttgt atcaaataca aaagtgttag
    48361 caacacaaac tgctcttact caaattgagg aggtgcttgc ataatgaact tactagacgt
    48421 aatcaaacgc ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca agaaatacac
    48481 ttttgaagtg gacactcgcg caaacaaaac tttagtaaaa caagcggtag aagctgcttt
    48541 tgacgttaaa gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg
    48601 caaatatgca ggatatacaa aaaaacgtcg caaagcgatc gttacattga ctgaagattc
    48661 aaaagaaatc caattattcg aagctgctga ataagcacta atagataact cattaaatta
    48721 ggagggaaaa acgtggcgat taaaaagtac aaacctacct caaatggtcg tcgtaacatg
    48781 acaggttctg attttgctga aatcacaaca tcaacacctg aaaaatcatt gttacaacca
    48841 ttgaaaaacc atgctggacg taacaacaac ggccgcatca ctgtacgtca tcaaggtggc
    48901 ggacacaaac gccaatatcg cgtgatcgac ttcaaacgta acaaagataa cgttgttgca
    48961 actgttcaaa cgatcgaata tgatccaaac cgttcagcta acatcgcgtt agtacactat
    49021 gaagatggag tgaaagctta catcttagca cctaaaggac tagaagtggg tatgaaactt
    49081 gtttcaggtc cagaagcaga tatcaaagtc ggaaatgctc ttccattaga aaacattcca
    49141 gttggtactg ttatccacaa cattgaaatg aaacctggta aaggtggaca attgatccgt
    49201 tctgctggta caagtgctca agtacttggt aaagaaggca aatacgtatt gatccgcttg
    49261 aactctggtg aagttcgtat gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt
    49321 aacgaacaac acgaattaat caacatcggt aaagctggac gctctcgttg gatgcgtaaa
    49381 cgcccaactg tacgtggtag cgtaatgaac cctaatgatc acccacacgg tggtggtgaa
    49441 ggtaaagctc ctatcggacg taaagcacca gtttctcctt ggggtcaacc agctcttggt
    49501 tacaaaactc gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat
    49561 aaataattac tttgaccatg tgtcgcacat tttgagagga ggttcaccat gggtcgtagt
    49621 ttaaagaaag gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg
    49681 gttgaaaaga aaaaagttat caaaacttgg tcacgccgtt caacagtttt cccaagtttt
    49741 gttggatata ccatcgcagt ttatgatgga cgtaaacatg taccagttta catccaagaa
    49801 gacatggtag gacacaaatt aggtgaattt gcacctacaa gaacttatcg tggacatgct
    49861 gctgacgata agaaaacgaa acgctaattt gaggggagga taaatccaaa tggcagaaca
    49921 aatcacatca gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt
    49981 agtaattgac cttatcagag gtaaaagtgt cgcagatgca atttcaatct tgaaattcac
    50041 accaaacaaa tctgctggaa tcattgaaaa agtattgatg tcagcagttg cgaatgcaga
    50101 aaataacttt gacttagatg ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg
    50161 acctacaatg aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg
    50221 tacaagtcat atcacggtag tcgtatcaga aaaataagga gggataatca gtgggtcaaa
    50281 aagtacatcc aattggaatg cgtgtaggca tcatccgcga ctgggatgct aaatggtatg
    50341 ctgaaaaaga gtatgctgag ttcttacacg aagatttaag aatccgtaaa tttatttcaa
    50401 ctaaacttgc tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa
    50461 acatttcaat tcacacagct aaaccaggta tggttattgg taaaggcggt tctgaagtcg
    50521 aaagcctaag aaaagaatta aacaaattaa ctggtaaaag aatccatatc aatatcgtgg
    50581 aaatcaaaaa accagatcta gatgcaaaat tagtaggtga aggaatcgca cgtcaattag
    50641 aaaaccgtgt tgctttccgt cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg
    50701 gcgctcaagg gatcaaaact caagtatctg gtcgtctaaa cggagcagat atcgctcgtt
    50761 cagaaggata ctcagaagga actgttcctc ttcatacttt acgtgcggac attgattacg
    50821 catgggaaga agcagataca acatacggaa aactaggagt taaagtgtgg atctatcgtg
    50881 gagaaattct tccaactaaa aaaaacactg agaaaggagg gaaataatca tgttagtacc
    50941 taaacgtgta aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg
    51001 aaaagaagta gcattcggtg aatacggttt gcaagctgtt gattcacatt ggatcacaaa
    51061 ccgccaaatc gaagctgctc gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt
    51121 atggattaaa attttccctc acaaatctta tactgccaaa gcaattgggg tacgtatggg
    51181 ttctggtaaa ggggcacctg aaggatgggt tgcaccagta aaacgtggta aaatcatgtt
    51241 tgaaatcgca ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa
    51301 attaccaatg aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg
    51361 ttaaagaaat cagagaatta accactgccg aaatgcttga tcaagaaaaa caattaaaag
    51421 aagaattgtt taatcttaga ttccaattag caacaggtca attagaaaac actgcacgta
    51481 ttaaagaagt acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa caagctaagt
    51541 aattatggaa ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc
    51601 aaggtcgcgt ggtttcagac aaaatggata aaacaattac cgttgctgtc gaaacaaaga
    51661 aaaaccaccc tatctatggc aaacgcatga actattctaa gaaatacaaa gtgcatgatg
    51721 aaaacaacac agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt ccattatcag
    51781 ctacaaaacg tttccgtttg ttggaagtag tcgaagaagc agttattatc taataacacg
    51841 agattttcct atatagattg aatgcaaaat ctgaaaggag gatacacaac gtgatccaag
    51901 cagaaagtcg tttaaaagtc gctgataact caggcgcacg tgaaattttg acgatcaaag
    51961 tccttggtgg atctggtcgt aaaactgcta acatcggtga tgtgatcgtt gctacggtta
    52021 aacaagcaac gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc
    52081 gaacaaaatc aggagctcgt cgtacagacg gttcatatat taaatttgat gaaaatgctg
    52141 cagtgattat tcgtgatgat aaaagccctc gtggaacacg tatctttggg ccagttgcac
    52201 gtgaactacg tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa gttttataat
    52261 cttgagacac ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag
    52321 ttaaagttat cactggaaaa gacaaaaaca aagaaggcgt tgtactagca gcgttcccga
    52381 aaaaagataa agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc
    52441 aagctgctcc tcaaggtgga attgttgaga tggaagcgcc aattcatgtt tctaacgtga
    52501 tggtggttga ctctactggt gtagctggcc gagttggcta caaagaagta gacggcaaaa
    52561 aagtccgtgt ttctaaaaaa accggtgaag ttttagataa ataaaagttt aaggaaggag
    52621 ggtcttactg aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact ccatcattga
    52681 tggaaaaatt taactatagc tctgttatgc aaacacctaa agttgaaaaa atcgtcatca
    52741 acatgggtgt tggtgatgcg gtatcaaacg ctaaaaactt agataaagca gttgaagaat
    52801 taactttgat cactggtcaa aaaccaatga ttacaaaagc taaaaaatca atcgctggat
    52861 tccgtttacg tgaaggaatg ccaattggtg caaaagttac attgcgcgga gaaagaatgt
    52921 acgaattctt agacaaattg gtttctgttt cactaccacg tgtacgtgac ttccatggtg
    52981 taagtaaaaa agcctttgac ggtcgtggaa actatacttt aggtattaaa gaacaattga
    53041 tcttcccaga agtagattat gatttagtag ataaagtacg tggtatggac atcgttattg
    53101 taacaacagc aaactctgat gaagaatctc gtgagttgtt gacacaatta ggcatgccat
    53161 tccaaaaata attaaaggag gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca
    53221 aacgtcctgc aaaattttca acacaagctt atactcgttg cgaacgttgc ggacgtccac
    53281 attcagttta tcgtaagttc catctttgcc gtatttgctt ccgcgaactt gcctataaag
    53341 gtcaaattcc cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat aagaaggagg
    53401 taaaagatca atggtcatga cagatccaat tgcagatttt ctaactcgta tccgtaacgc
    53461 aaacatggtt aaacatgagt ctttagaagt acctgcatca aaaattaaac gtgacatcgc
    53521 tgaaatcttg aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca
    53581 aggcgtgatc cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta tcacaaactt
    53641 aaaacgtatc tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt
    53701 attgaacggt ttaggtatcg caatcatttc gacttctgaa ggtgttgtca ctgataaaga
    53761 agctcgtgaa aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat caagtagaat
    53821 ctacaaggag gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt atccctgaag
    53881 gagtaacagt tactcaagac ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga
    53941 ctcgtacttt ttcagcagat atcaaaatga atatcgaagg aaacgaagta actttcactc
    54001 gtccaaacga cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca
    54061 acatggttgt aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt
    54121 accgtgcaca attacaagga tcaaaacttg tattgaacgt tggttactca catccagtag
    54181 aaatcacacc accagctgga gtaactgttg aagtaccagc taacacacaa gtaattgtta
    54241 aaggtgcgaa caaagaagaa gttggcgaat tagctgcaaa cattcgtgga actcgtcctc
    54301 cagaacctta taaaggtaaa ggtattcgtt atgttggcga atatgtacgc cgtaaagaag
    54361 gtaaaactgg taaataatcg tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc
    54421 atttggctga agtttacctt caaaaaaata catttttact tgtgccgcag cttctatttt
    54481 tcgaaacgta tttactgctt gaaatagtag tacaataaaa ataaagaggt gacaattgtg
    54541 attacaaaac cagataagaa caaaacacgt caaaagagac atcgtcgtgt acgtaacaat
    54601 atctctggta ctgctgagcg cccacgcttg aacgtttttc gttctaacaa aaacatctac
    54661 gctcaaatta ttgatgacgt agcgggtgta acgctagcaa gtgcctctac cttggataaa
    54721 gaaatttcag gtggaacaaa aacagaagca gcacaagctg tcggtaaact agttgctgaa
    54781 cgtgcagctg aaaaaggcat taaagtagta gtctttgacc gtggtggata cctttaccat
    54841 ggccgtgtgc aagctttagc tgaagctgct cgcgaaaatg gactagaatt ttagaagaag
    54901 gaggaatacc attcatggtt tatatcgatc caaaacattt ggaattagaa gaccgcgttg
    54961 ttgcaattaa ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt tttgctgctt
    55021 tagttgttgt cggtgacaaa aacggacacg tagggtttgg tacaggtaaa gctcaagaag
    55081 ttcctgaagc aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt gaagtaccta
    55141 tggttggttc aacaatccca cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa
    55201 tgaaacctgc tgtagaaggt tctggggttg ctgctggtgg accagttcgt gccgtattag
    55261 aattagctgg ggtagcagat atcacttcta aatctttagg ctcaaataca ccaatcaacg
    55321 ttgttcgcgc aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac
    55381 ttcgcggcaa atcagttgaa gaattaatcg gataaggagg acaacaataa tggctgaatt
    55441 aaaagttact ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg atacagttaa
    55501 agcgttaggt cttacaaaga ttaacagttc agtggttaag ccttctaatg aagcaattaa
    55561 aggcatgatc aatactgttt ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat
    55621 cataactaga acattaagga ggtgccaaac ctatgaaact tcatgaatta aaacctgctg
    55681 aaggttcacg ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa
    55741 ctgccggacg cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat
    55801 ttgaaggggg acaaacacca ttgttccgtc gtttaccaaa acgaggattc acaaacgtga
    55861 accgtaaaga ctatgcagtc attaatcttg atgtattgaa ccgctttgaa gacggagctg
    55921 aagtaactcc tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca
    55981 aagtattagc taatggggaa ctaaacaaga aattaactgt gaaagcagca aaattctcta
    56041 aagcagctca agaagcaatt gaagcggctg gtggatcaat cgaggtgatc taatgtttag
    56101 actattaaaa gatgctttta aagtaaaaga cattagatcg aaaatcctat ttactgtatt
    56161 tgtgttgttt gtatttcgcc taggtgcaca tattactgta ccaggcgtaa atgctgcttc
    56221 attgcaaaac ctttcttctt tgccattttt caacatgttg aacttggtaa gtggtagtgc
    56281 gatgcaaaac ttttccattt tctcaatggg ggtttcacca tacattactg cttctatcgt
    56341 cattcagctt cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga aacaagggga
    56401 agttggtcgt aaaaaactga atcaagcgac tcgatatctg acattagtat tggcatttat
    56461 ccaatctgtc ggtatcactg ctggattcaa tcaatacgct caattaggtt tcgtgaataa
    56521 tcctaacgta gcaacttacg taacgatcgg tttgattcta acagccggta cgatgtttgt
    56581 tacttggcta ggagaacaga tcacagaaaa aggtatcgga aatggcgtat caatgatcat
    56641 ttttgcaggt atcatttcta gattgcctga aagtatcaga gaaatttatg aagattactt
    56701 tataaatatt gatccttcag acatatggaa atctgctatt tttgtggcca ttttactagt
    56761 agcggtcttg ttgatcgtga cattcgtaac atttttccaa caagctgaac gcaagatccc
    56821 gatccaatat acgaaacgcg tagctggcgc gcctacaagc agctatctgc cgttgaaagt
    56881 aaatgcagct ggagttattc cagttatctt tgctagttcg tttattgcta cgccaaatgc
    56941 tgttttacaa gcattgagcg gtagattcgg ttctgaaaac tggtacaaag tgatgatgga
    57001 gatctttaac tataatacag cacctggtgc tacgatttat acgattctga tcgttgcctt
    57061 cactttcttc tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa
    57121 gcaaggaagt tatataccaa gcgtacgccc tggtaagggg actgaagagt atgtctcaag
    57181 attactgatg cgtttaagta cagttggtgc agtcttcctt ggtttagtgg cattgcttcc
    57241 aatcattgct caaatggttt gggatcttcc acaatcaatc ggattaggcg gtacaagctt
    57301 actgatcgtt atcggtgtag ctttagaaac tgcgaaacag ttagaaggct taatgctgaa
    57361 acgtaaatat gttggattca ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg
    57421 ttctcacaca tttattagga gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg
    57481 caggtaaagg aacacaagct gaaaaaatca ttgatgctta tggtattccg cacatctcaa
    57541 caggagatat gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt
    57601 cttatatgga taaaggtgca ttagttcctg atgaagtaac aaatggaatc gtgaaagagc
    57661 gactagctga accagataca gagaaaggct tcttattaga tggctttcca cgtacgctag
    57721 atcaagcaaa agctctggac gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca
    57781 tcgatatcca tgttggtgaa gaaatcttgg tcgaacgtct agccggccga tttatctgca
    57841 gcaattgtgg agcaacttac cataaaattt tcaaccctac aaaagttgaa gacacatgtg
    57901 atcgttgcgg tgggcatgaa ttctatcaaa gagaagatga taaacctgag acggttaaaa
    57961 atcgtctggc tatcaacatc aaaaatagtg aaccaatctt ggcttattat aaagaacaag
    58021 gtttgctaaa cactattgat ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa
    58081 aaatcattga aaaatagcac gattgcccgt agccgacttg cattatgcag ttactcttgt
    58141 caagttcaat aaattatgat agaatatgtc agtccgactt ctagagaatc ctctagatga
    58201 tccattttga gaattttgag gtgggaactg cagtttccgc cattttatcg tgtgaaaatg
    58261 cgaaacaagt acaaggaggt actgtgcgtg gcaaaagaag atatgattga agtcgaaggt
    58321 acagtcgtcg aaactttgcc gaatgcaatg tttaaagttg aactagaaaa cggacaccaa
    58381 gttcttgcta ctgtttcagg taaaattcgt atgcattaca tccgtatctt acctggtgac
    58441 aaagtaacgg ttgaattgtc tccatacgat cttaaccgtg gccgtattac ttatcgcttt
    58501 aaataattgt actccgtaaa actacaggag gtattatcat gaaagtaaga ccatcagtaa
    58561 aaccaatgtg cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc
    58621 cagcaaatcc aaaacataaa caacgtcaag gataattgga ggtgctataa atatggctcg
    58681 tattgcagga gtagatattc ctcgtgataa acgtgtagta gtttcactta cttatatcta
    58741 tggtattggt aacactactg ctaaaaagat cttagctgat gctggtgtat ctgaagatgt
    58801 tcgcgttcgt gaattaacga atgaacaaac agatgctatc cgtgcagaag tagataaatt
    58861 aaaagttgaa ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat
    58921 cggttcttac cgtggaatcc gtcatcgccg tggattgcct gttcgtggac aaaacacgaa
    58981 aaacaatgca cgtactcgta aaggcccagc tcgcgctatc gctggcaaga aaaaataatt
    59041 cttaagtgaa ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt
    59101 gaagaagaat atagaagcag gtattgcaca tatccattct acattcaata acactatcat
    59161 catgatcact gatactcacg gaaatgcatt agcgtggtca tcagctggtt cattaggttt
    59221 caaaggaagc aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa
    59281 agcagcaatg gaacacggat taaagactgt tgaagtaaca gttaaaggac ctggttctgg
    59341 acgtgaagca gcaattcgtt cattacaagc tacaggttta gaagtgactg caattcgtga
    59401 cgtgactcca gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt
    59461 gccgctcatt ttgagtttta tcagtaacgt cattgaacaa gacacttttc gttttgaaag
    59521 gggtaaagat aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa
    59581 aagattatgg caagttcatc gttgaacctc ttgaaagagg gtatgggact actttaggga
    59641 attccctacg tcgtatttta ttatcttctt tgccaggtgc tgccatcact aatattcaaa
    59701 tcgatggtgt actgcatgaa ttctctacca tcaaaggtgt tagagaagac gtcactcaaa
    59761 tcatcttgaa cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg
    59821 aaatcgatat taccggacct gctacagtaa ctgctggcga tattatcgtt gatagtgatg
    59881 tagaaattct taacaaagat atgtacatct gtagtgtctc tgaaggagct acgttccatg
    59941 ctcgcttaac agtgaaacct ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag
    60001 atatgccaat tggcgttctt ccagtcgatt caatctacac acctgttcgt cgtgtgaact
    60061 atcaagttga gaacacacgt gttggacacc gtgaagattt tgataaattg acgatggaga
    60121 tttggacaga tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga
    60181 ctgaacattt ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg
    60241 tcgaaaaaga agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa gagttagact
    60301 tgtctgttcg ctcttacaat tgtttgaaac gtgcaggaat caatactgta caagaactta
    60361 caaataaatc tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa tcattagaag
    60421 aagttaaagc aaaattgcac gacttaggtt taggtttacg taaagaagat taaaaccttt
    60481 tacgaaggag ggaaaccaac gtgagttatc gtaaactagg acgcacatct agccaacgta
    60541 aagcgatgtt gcgtgattta acaactgatt tattaatcaa cgaacgtatt gtgactactg
    60601 aagctcgtgc gaaagaagtt cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg
    60661 gagatttgca tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc
    60721 gtgaagaaaa tgaggacatc gtcattgaat cagctttaca aaaactgttc aacgatattg
    60781 cacctcgtta cgcagaacgt caaggcggat acacacgcat tttgaagaca gaaccaagac
    60841 gcggtgatgg tgcaccaatg gttgtcttag aacttgtcta aaatcacact aataacatca
    60901 ctttatagat gattctttta gagtgttatg atgttggaag atatttttcc gagtctagct
    60961 cagcgctgct cctcaagcga atatcgcttg gggagtaagc actcatcata aagtgttttt
    61021 ttataccttc aattacctgt catgacagat atgtttgaag gcataaaaaa acattttaag
    61081 cgagaaataa ttgaaaattt tgtctttttt tgatagtgtt agtaggaact ttaggatgaa
    61141 gaaggagtaa tcatggagcc aattattgaa ttagaaaaga taaattataa ataccagcca
    61201 gatgatcttc gccccgcatt gaaagatgtc tcctttacaa ttgataaagg tgagtggatc
    61261 gctattattg gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat caatggattg
    61321 cttctgccag aatcagggat cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt
    61381 tggacgatcc gacagatggt tggaatgatt tttcaaaatc cagacaatca gtttgttggt
    61441 tcgacagtag aagatgacgt tgcttttggt cttgaaaatc aagggattcc cagagaagaa
    61501 atgctagtgc gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt tgcttcacgc
    61561 gagcctgctc gtttatcagg agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc
    61621 ttacgacctg atatcattat tttagatgaa gcaaccagta tgctagatcc agaaggtcgt
    61681 gaagaagtga tctctacgat caagaagatc aaagaagaaa gtcaattgac cgttatatct
    61741 atcactcatg atattgatga agcagctaat gccaatcgga tactagtgat gagacaaggc
    61801 gaacttgtcc gtgaaggtac accaaaagaa attttttctg ctggtcctga actgatcgac
    61861 ttaggtctag atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt
    61921 gatgtgccca gtgaatatat gacagaagaa aggatggtgg attggttatg gacatccgtt
    61981 ttgaacaagt agacttcact tatcagccga atacaccatt tgaacaaaga gccttatttg
    62041 acatcaatat gacgatcaaa gaaaacagtt atactgcatt agtagggcat accggaagcg
    62101 ggaaatcaac cttacttcag catttgaatg cgcttgttaa gccaacaagc ggtacagtcc
    62161 atattggaga acgagatatt cagccggata cggataataa gaatttaaag cccatccgaa
    62221 aaaaagttgg catcgtcttt cagtttccag aagcacagct attcgaagaa acggtagcaa
    62281 aagacattgc tttcggtcct aaaaactttg gtgtcagcga agaagaagca ctagtcctag
    62341 caaaagaaac attagaacaa gttgggctgg atgaaagcta tttggaacgt tcgccatttg
    62401 aactttcagg agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg
    62461 aagtacttgt attggatgaa ccaacggcag gacttgatcc ccagggcaga aaagaaatga
    62521 tggagatgtt ttggcgtctt cataaggaac agcagatcac tattgtatta gttacgcatt
    62581 taatggatga tgtagccaat tttgcagatt atgtctatgt gttggaaaag ggacgaatcg
    62641 ttaatagcgg ggagccgcaa gaggtctttc aaaacattga atggctaaaa gaaaaacaac
    62701 taggcgtacc tactgccacc gagtttgcag aagaattaat ggctaaaggg atgaactttg
    62761 ctaccttgcc tttgacagct gaagaattag cagacgctat cgtccattat gctggaggaa
    62821 atgcccatga tgaataaatt gatatttggt cgttatatgc ctggagattc cttgatccat
    62881 cgattggatc cccgagctaa attattagcc agtttttatt ttatcggaat cattttttta
    62941 gcaaataatt gggaaacgta ccttttgttg gcatttttca cattactttc tgtcttcttg
    63001 tcaaaagtag atttgaaatt ttttatccga ggcattcgcc cattgatctg gctaatcctc
    63061 ttcactgtag ctttgcaggt cttgtttaca agtggcggag aaacttattg gagttggggt
    63121 gttttccata tcactgaatt tggtgtgatg aacggcctct ttattttctg tcgattcgtg
    63181 ttgatcattt ttatgtcgac tctactcacg cttactacac cgcctttgga attatcggat
    63241 gcgattgagt acttgcttcg tccattgaaa gccgtacggt tcccagtcca cgaaatttca
    63301 ttgatgctgt cgattgctct tcgttttgtg cctacattga tggacgaaac agaaaagatc
    63361 atgaacgctc agcgcgctcg cggtgttgat tttggtgaag gaaacctagt tcagaaaatg
    63421 aaagcagtca ttcctttgct gattccttta tttgtcagca gcttcaaccg tgcggaagat
    63481 cttgcgactg ctatggaagc aagaggctac aaaggtgggg aaggaagaac gaaatatcgt
    63541 gtattgcatt ggcataatag agacacgatg gttatcattg cttttgtatt attaactgtg
    63601 ctactgatct ttttaagagg ataaacgttc agtgggccgc tatctattct tttcttgctg
    63661 atctgtttgc aagaaaggat gcaagcagtg tatgacaaga aatattaggg tctgtggcag
    63721 actaaggtca caggcctctt tcttttatct gaaaagaaag aacgaaaaaa ttttatagaa
    63781 aaatcattat tccagataac cttttaggaa caaaggagag aaaaacatgg tacgttacaa
    63841 agcaatcatt gcgtacgacg gaacaaattt taatggattc caaaaacagc caaatgggcg
    63901 tactgttcaa gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac
    63961 ggtctttggt tcgggaagga cagatgccgg agtacatgcg atcgggcaag taatccattt
    64021 tgattatcca gaggagcgtc cgttagaacg aatgcgtttc gcgctagata cccagtcacc
    64081 agaggatatt gctgtaagac aagtagaaat tgtttcagaa gacttccacg caagatattt
    64141 ggtcaaagaa aagacgtatc aatttcgagt ggatataggg aaaccacgca gtccttttcg
    64201 acgccattat gcaagttatt ttccttaccc acttgatttg gaaaaaattc agcgggcgct
    64261 tcctgattta ttagggactc acgatttcac ctctttttgt gcatcaggaa gctcgataga
    64321 ggataaaata cgtacgatct acgaggctaa gatggaggta aatgaaacaa aagacgaatt
    64381 gttgtttaca tttaggggaa atggcttctt gtataagatg atacggatct tagtaggaac
    64441 gcttttgaaa attggcaatg gccgattgcc tgaagatagt atcccagata ttattgcgaa
    64501 aaaagatcgt aatgcagcag gacctacagc acaccctgaa ggactttatt tgtatgaagt
    64561 gaaatatgaa taagaggtta tgatagaagt ggtcagcttc aagaaataag aaggtattta
    64621 cgaaaattac ttctcaaaat tttgcaaaat ttcggcttat tttcgaagaa gctacttctg
    64681 gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca caaagagcaa
    64741 tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga ggcgttgata
    64801 agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt
    64861 tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt ttctacgtct
    64921 tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc cataaaaggg
    64981 gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata ggacaaaaag
    65041 gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat gtttacctgc
    65101 tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg gtttcccatc
    65161 aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg aatcttcagc
    65221 ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac ctttttcttt
    65281 gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt taggctccat
    65341 gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg tctcttgagg
    65401 ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc gcgatgtacc
    65461 attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg ggaacaaact
    65521 taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt ttggctggtt
    65581 atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg tattcaactg
    65641 attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag acaatgtatt
    65701 gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat cattaacgga
    65761 caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg tatagtcgcc
    65821 gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt tagaaatgtt
    65881 atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac ggatattcaa
    65941 atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat tacgatttct
    66001 tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt ttgcagatac
    66061 tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt catttacaag
    66121 agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat tccaatcatc
    66181 aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca tgatcaataa
    66241 taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc ccacagcaaa
    66301 agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca cataggtgta
    66361 gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga ccaaaatgat
    66421 tcctaacaac agcccaccat cgtcttgttc tatcttttcc catccttttc ctgcaagcag
    66481 gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg gtgcatgcat
    66541 accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta tggcgaaaag
    66601 actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat aaaacagaca
    66661 gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt ttgtcgtgtc
    66721 ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt ctgttttcaa
    66781 cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg ctggcaatac
    66841 aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa caatcgtttt
    66901 tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag aaaatacgcc
    66961 aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga taaataaaag
    67021 tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg gaagataaac
    67081 aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga tcaatgcgta
    67141 actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg caagaaacca
    67201 aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg catctggcat
    67261 catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat agtaagaaat
    67321 taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac tttgctttcc
    67381 aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg catcacttgc
    67441 caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa gtgctaaaat
    67501 caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa agaatgactt
    67561 taaatttttt ttcatattta ttttccacca aatttctatg aagttgtatt cagttt
//
LOCUS       DAZUKR010000004        49345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000004 DAZUKR010000000
VERSION     DAZUKR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.004"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..1112
                     /locus_tag="ACG89C_000256"
     CDS             111..1112
                     /locus_tag="ACG89C_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS4898843.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1131..2150
                     /locus_tag="ACG89C_000257"
     CDS             1131..2150
                     /locus_tag="ACG89C_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS4898844.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2252..2968)
                     /locus_tag="ACG89C_000258"
     CDS             complement(2252..2968)
                     /locus_tag="ACG89C_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4898845.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(2937..3722)
                     /locus_tag="ACG89C_000259"
     CDS             complement(2937..3722)
                     /locus_tag="ACG89C_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4898846.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3713..4462)
                     /locus_tag="ACG89C_000260"
     CDS             complement(3713..4462)
                     /locus_tag="ACG89C_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4898847.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4760..5284
                     /locus_tag="ACG89C_000261"
     CDS             4760..5284
                     /locus_tag="ACG89C_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4898848.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5300..6799
                     /locus_tag="ACG89C_000262"
     CDS             5300..6799
                     /locus_tag="ACG89C_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4898849.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6887..7489)
                     /locus_tag="ACG89C_000263"
     CDS             complement(6887..7489)
                     /locus_tag="ACG89C_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4898850.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7593..8549
                     /locus_tag="ACG89C_000264"
     CDS             7593..8549
                     /locus_tag="ACG89C_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS4898851.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8854..9810)
                     /locus_tag="ACG89C_000265"
     CDS             complement(8854..9810)
                     /locus_tag="ACG89C_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4898852.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9984..10544
                     /locus_tag="ACG89C_000266"
     CDS             9984..10544
                     /locus_tag="ACG89C_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898853.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10537..11361
                     /locus_tag="ACG89C_000267"
     CDS             10537..11361
                     /locus_tag="ACG89C_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS4898854.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11371..11988
                     /locus_tag="ACG89C_000268"
     CDS             11371..11988
                     /locus_tag="ACG89C_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4898855.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12006..12689
                     /locus_tag="ACG89C_000269"
     CDS             12006..12689
                     /locus_tag="ACG89C_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4898856.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13372..14607
                     /locus_tag="ACG89C_000270"
                     /pseudo
     CDS             13372..14607
                     /locus_tag="ACG89C_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            14615..15502
                     /locus_tag="ACG89C_000271"
     CDS             14615..15502
                     /locus_tag="ACG89C_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4898857.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15519..16355
                     /locus_tag="ACG89C_000272"
     CDS             15519..16355
                     /locus_tag="ACG89C_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4898858.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16358..16981
                     /locus_tag="ACG89C_000273"
     CDS             16358..16981
                     /locus_tag="ACG89C_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898859.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16978..18270
                     /locus_tag="ACG89C_000274"
     CDS             16978..18270
                     /locus_tag="ACG89C_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4898860.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18629..18726
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18842..19423
                     /locus_tag="ACG89C_000275"
     CDS             18842..19423
                     /locus_tag="ACG89C_000275"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4898861.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19426..20733
                     /locus_tag="ACG89C_000276"
     CDS             19426..20733
                     /locus_tag="ACG89C_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS4898862.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21026..21514
                     /gene="purE"
                     /locus_tag="ACG89C_000277"
     CDS             21026..21514
                     /gene="purE"
                     /locus_tag="ACG89C_000277"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4898863.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21507..22631
                     /gene="purK"
                     /locus_tag="ACG89C_000278"
     CDS             21507..22631
                     /gene="purK"
                     /locus_tag="ACG89C_000278"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4898864.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22644..23939
                     /gene="purB"
                     /locus_tag="ACG89C_000279"
     CDS             22644..23939
                     /gene="purB"
                     /locus_tag="ACG89C_000279"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4898865.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24119..24496)
                     /locus_tag="ACG89C_000280"
     CDS             complement(24119..24496)
                     /locus_tag="ACG89C_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898866.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24752..25480
                     /gene="purC"
                     /locus_tag="ACG89C_000281"
     CDS             24752..25480
                     /gene="purC"
                     /locus_tag="ACG89C_000281"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4898867.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25480..25734
                     /gene="purS"
                     /locus_tag="ACG89C_000282"
     CDS             25480..25734
                     /gene="purS"
                     /locus_tag="ACG89C_000282"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS4898868.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25736..26407
                     /gene="purQ"
                     /locus_tag="ACG89C_000283"
     CDS             25736..26407
                     /gene="purQ"
                     /locus_tag="ACG89C_000283"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS4898869.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26408..28630
                     /gene="purL"
                     /locus_tag="ACG89C_000284"
     CDS             26408..28630
                     /gene="purL"
                     /locus_tag="ACG89C_000284"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS4898870.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28615..30054
                     /gene="purF"
                     /locus_tag="ACG89C_000285"
     CDS             28615..30054
                     /gene="purF"
                     /locus_tag="ACG89C_000285"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4898871.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30077..31129
                     /gene="purM"
                     /locus_tag="ACG89C_000286"
     CDS             30077..31129
                     /gene="purM"
                     /locus_tag="ACG89C_000286"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4898872.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31126..31704
                     /gene="purN"
                     /locus_tag="ACG89C_000287"
     CDS             31126..31704
                     /gene="purN"
                     /locus_tag="ACG89C_000287"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4898873.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31701..33236
                     /gene="purH"
                     /locus_tag="ACG89C_000288"
     CDS             31701..33236
                     /gene="purH"
                     /locus_tag="ACG89C_000288"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4898874.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33252..34526
                     /gene="purD"
                     /locus_tag="ACG89C_000289"
     CDS             33252..34526
                     /gene="purD"
                     /locus_tag="ACG89C_000289"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4898875.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34648..35562
                     /locus_tag="ACG89C_000290"
     CDS             34648..35562
                     /locus_tag="ACG89C_000290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4898876.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35695..37260)
                     /locus_tag="ACG89C_000291"
     CDS             complement(35695..37260)
                     /locus_tag="ACG89C_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898877.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37253..38704)
                     /locus_tag="ACG89C_000292"
     CDS             complement(37253..38704)
                     /locus_tag="ACG89C_000292"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4898878.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38718..39734)
                     /locus_tag="ACG89C_000293"
     CDS             complement(38718..39734)
                     /locus_tag="ACG89C_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4898879.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            39954..41042
                     /gene="chvE"
                     /locus_tag="ACG89C_000294"
     CDS             39954..41042
                     /gene="chvE"
                     /locus_tag="ACG89C_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4898880.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41096..42622
                     /gene="mmsA"
                     /locus_tag="ACG89C_000295"
     CDS             41096..42622
                     /gene="mmsA"
                     /locus_tag="ACG89C_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4898881.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42624..43829
                     /gene="mmsB"
                     /locus_tag="ACG89C_000296"
     CDS             42624..43829
                     /gene="mmsB"
                     /locus_tag="ACG89C_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS4898882.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44086..45063
                     /gene="guaC"
                     /locus_tag="ACG89C_000297"
     CDS             44086..45063
                     /gene="guaC"
                     /locus_tag="ACG89C_000297"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4898883.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45327..46751
                     /locus_tag="ACG89C_000298"
     CDS             45327..46751
                     /locus_tag="ACG89C_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4898884.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(46793..47050)
                     /locus_tag="ACG89C_000299"
     CDS             complement(46793..47050)
                     /locus_tag="ACG89C_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898885.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            47212..48321
                     /gene="pdhA"
                     /locus_tag="ACG89C_000300"
     CDS             47212..48321
                     /gene="pdhA"
                     /locus_tag="ACG89C_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS4898886.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48324..49301
                     /locus_tag="ACG89C_000301"
     CDS             48324..49301
                     /locus_tag="ACG89C_000301"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS4898887.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        15733 a         8503 c        10861 g        14248 t
ORIGIN      
        1 aaaataaaag ctttttttat tgtgaataaa tgaacataat taggaggaga aagatgaact
       61 tatttgacat ttttcgatcg actttgacag atatgtcgat tgtcagtgca atcacttcaa
      121 cagtttttat tatattatta gggtttgttt gtagaaaaaa ggggattttt tctgaatctg
      181 ttggaaaaat tctatcgaaa gtcgtcttaa cagtggcttt gccagcactg gcatttcgag
      241 ctttcatgca agatatcaat acagaattac tgatgcaagg aatcaatgtc ttgatttggg
      301 gattagttat ttatattatt cttattctag tttcaaaacc ttttttctta agctataaag
      361 gggataagca aacaacgttg cgtgtcctga cgatctttgg ttctacgacc tttttcggaa
      421 cgcctatcgt cagtgcgatt tatggtcctg tcggcgtgat gtattcctca attttcaata
      481 ttggctaccg aatctttttg tattcttatg gatacataaa aatgagtggc ttgaagatgg
      541 aagctaaaaa tatcaaagcg atgttcgtca atccaatcat tatcgctact tttgccggcc
      601 ttttcatttg gctgttccaa gggtatctgc cacaagtgtc agttacaggt gcagaaggag
      661 agataagcca agtagcgttt ttgcgaattg accagactgc accgtggcta tttcagccga
      721 tggattattt agctggttta agttcaccat tggcgtggct agcaatcggc gcaacattag
      781 gtgaagtaag cttcaaaaaa gctgcatctg aaaaaacttc atggtattac agtctagtca
      841 aagtggtatt tgtacctgca ttgaatatcg tgattctagc agttttgacg gcaacgaata
      901 ttctaccagt caattttgaa gcactggcaa caatcgttat catgatggct acaccagctg
      961 caacagtggc agcagcctat gctatcagtt ttgataggga ggcactttta gcctccaatg
     1021 catccttact gtcaactatt tttgctgtgg tactgacacc tgtatggatc gttgtattac
     1081 aaatgattca tggcataggt ttattttcat aagaaatgga ggaagaaagt atgagaatcg
     1141 catgttacgg tgttcgtccg aacgagatag atttttttga aaaattgaat atttaccatt
     1201 atgatctcag tctttatgaa gaactgttga cacatgacaa tatcgtgtca gcaaaggacc
     1261 atgatgctgt attgttgagg ggaaattgta tagcagatga gaccaatctt gcaaaaatgc
     1321 aggaatatgg gattcgttat gtttttacac ggactgtggg agtcaatcat atcgatctac
     1381 aagcggcagc tgattttggt atgacagtag cccgtgtacc atcgtattcg ccaaatgcga
     1441 tagcagagtt atctttgact tttgccatga tgcttttgag aaatactgcg tatacgacga
     1501 tccgaacatc tttcaaagat tttcgagtgg atgagcaaat gtttagtcgt gaaatcagaa
     1561 attgcacagt agggatcata ggaacaggca ggatagggtt gacggaagct gttttgttta
     1621 agggactagg agccaaagtg atcggctacg atattcatca gacagaagct gctaaaaaag
     1681 tattgacgtt ccactcgctt gaagagttat tagccaaaag cgatattgtc agtattcatg
     1741 tacctcatat cccaggtgaa aacgatcaaa tgatcaatga aggattttta gctcaaatga
     1801 aaaaaggtag catcttgatt aatacagccc gtggagagct tcaagataat gaagctattt
     1861 taagagcttt gaaaagcaat catttagctg gttttgcaac cgatgttttt gcaaatgaaa
     1921 cagaggtttt tttccgatcc ttcaagccat gggagacgat tccagaccct gctatccagc
     1981 aattagtaga aatgtaccct agagttcttg taaccccgca tgtaggttca aatacagatg
     2041 aggcattatc aaatatgatt tcgacaagtt tcgagaactt tcgtgaaata atagaaactg
     2101 gaaaaacgaa aaatgaggta tctcttccta aagcccgtca attaagataa gaaaacatgg
     2161 atattcgtca ataaaatgtt taaaaatagt ccaagtaaac agttgttact tctgtatact
     2221 tggactattt tgtatgggat aattcgatga ctcaacagta atctgtgagc tttttccgcc
     2281 cattctttgt caattcataa caggttagcg gctggccgat ttccaaatat ttgattcttt
     2341 cgttcaatat atcttgttct actaaaaaat tcaaatattt cttgacggat atacgtgaca
     2401 gtgagctttt ttgagccagt tcttttgttg aaaaaatagt atcttctttt agaataaatg
     2461 cacttatttt ttttaaagtc agttttgaca gacctttagg caaatcgatg aatgatggta
     2521 tttcgtgaat gggagtggtg tggttgaata cttgatcgat caatttctga tcggcttttt
     2581 cagtcgtttg gattgtctga gagaaagcaa gatacttttc gatcgccagc tgaaaacgtt
     2641 gcaaagtgaa cggtttgatc aaataatcta aggcaccgta gcggatcaca ttctttatgg
     2701 tttcgatatc atctgcagca gtgatcaaaa tcactgggat atcatttcct tgctgtctaa
     2761 gccaagataa aaaatggata ccactttcgc caggcatgta tacatcaagc agcagcagat
     2821 ctgcgttttt ttgtctgaaa tatttttttc cttcttctac atttctgaaa ctttcgatta
     2881 cgttcatatt agggatgctt tccacaaaag tacgattcaa ttctgcaacc attggatcat
     2941 cttcgattat caggacattt gtcatcattt tgatcttcct tttttggtaa ttctagatag
     3001 acgattgtac cgccatttgt ttgtttggaa aattctaaaa atccaccgtg gcgtttgacg
     3061 attgaatgga tgagattcaa tccaaaccca cgattgattc ccttcgtcga aaaacctagc
     3121 gtaaatattt tttctgagat ttcttctgat atcccgacac cgccatcgca gacggtcacg
     3181 atgaatactt cagcttctgt gtcatagttt aaatgaatcc ggacctgtcc gtttctttca
     3241 tgtttttgta gcacggcttc ttttgcatta tcaagtaagt tccctaacac ttgcaaaagt
     3301 gaatggatgg agtcatccat ctccatttta gggataaaag aatcgctttc taacagcatt
     3361 tcaacagatt gttctttggc ttcgtttatt ttgcctaata aaaaaccggc aattgcaggt
     3421 actttgatca gacttgagac ttggccaatc tcttttttat aattttgatt tagctgctgg
     3481 ataaaatggg tgacttcttc atacttcttc aaatcaatca aaccagaaat gacatgaagt
     3541 ttattcatga attcatgggt ttgagctctc atggagttta tatattgttt cgtcccccca
     3601 agctcctcca ttaatttcat tacttctgat cgatttcgta aagttgcgac tgctcctaaa
     3661 aaacggtcat tgctaaaaag aggtgtaacg ctagaaatag tttctatccc atttaaatac
     3721 atggattgag tccgaacggt cgtttgatgc tcaatcgtct ctttaaacaa aacgtcatat
     3781 agattatcac tgattttttt cctacttcga aatccaatag ctgctctgtt tggtagaaaa
     3841 tgttttttgc aggtgtattg atcaagagga tagttttatc agaagagatg gctaagattc
     3901 cttcatctac ttcattttcg ataatcgttt tttcacttaa ctgttctgct atttcttgtg
     3961 gttcaagtcc aagcagtact tttttcattt tttgggcaag aaaaacggcg cctatgatgc
     4021 ctacgctaag tccaataatc aatgaaaaaa caatcatttt ttgtccatct ttgacttgat
     4081 tgtcaatcgt gttgtttgtt aagccaacac aaactgcacc aataatctct tgatcataat
     4141 aaatagggac gaaatagcgg aagccgaacc ctaacattcc ttctttttca gagaaaaatt
     4201 cttgtcctcc aatcactttt ttcacatctt catgacttga gaaaggaaga ccgatgtaac
     4261 ttgggtctgc gtgagaatat cgaatgcctt cagggtccat gacaacgata aaatctaaat
     4321 tcgatgcaat catgatttca tttgttattc cttgaatcgc atgtgtcggg tcaccagtct
     4381 gcagcttttt ttggatgagt ttatcattgg caacgatttt agcaatgcta gagattttat
     4441 ctgacacact agatcggttc atcgaaagaa taaaattcct gatcaatatg gcagaaacaa
     4501 tcaaagaaac gaatgtggag aaacaaacga caaggatgat cgtgttctgg agctttaatg
     4561 gttttttctt aaaaaaagga aacataggct acctcgaaag aatttattgt gaaaatttta
     4621 acatgaaaac aaaaatttaa caaattgtca acgataaaca caaccttcct ataaaaaaca
     4681 ccactgagtc ggtaaaagaa gctatacaga attttcaaac attggtatga tagacgtaga
     4741 aatggaagga ggcgtgtata tgataggggg aatcgcactt gagacgattt attggtatac
     4801 attgctgatt tgtgcaggat tggcagtcct attgattatt tttggagatg tattcgattt
     4861 tgatggcccg cttgatccga tgcttattat cccttggtta actttcacaa gcttatttgg
     4921 ttttctaggt gagcgtttaa cagatcaatc atctttattt ttatttattg ctagtggagg
     4981 catatcgata gtgttggtat ttttgcttaa tttctatata ttgatgccga tgaaacatgc
     5041 cgaatcaacg atgtcgtctt ctgaaaaaac tctggaagga caagtagctg ttgtgatcac
     5101 accgatccct gtacgaggta tgggagaaat tcagttgaaa agtgttaccg gttccattag
     5161 tcgtccagct tgtttttaca gcccccaaga acagacagct gagcggggaa cgaaagtatt
     5221 gatcattgag atcaaggaaa gaatctgtta tgtcatgcct tatgaaagca tgataaaagc
     5281 ttaaaggagc gtgtgttaaa tggaaatacc agttgttttt atcgcgatcg caatcgcagc
     5341 tttgatttta ctgatgttgc tgatcgtctt tgtagcgaag taccagacag caaaaccgga
     5401 tgaagcgttg atcatcagtg gaagttattt aggaagcaaa aatgtgcaca ttgatgaaag
     5461 caataacaaa atcaaaattg tgcgtggagg tggcgcattc gtcttgccag tttttcaacg
     5521 ttcgaatcgg atcagtctat tatcgagtaa attggacgtc tccacccctg aagtgtatac
     5581 cgaacaaggc gtaccggtga tgtgtgatgg tacttcgatc atcaaaatcg gttcatctgt
     5641 agaagagatt gctacagcag cagaacagtt tttaggcaag acaagagaag aactggaaaa
     5701 tgaagcaaga gaagtattag aaggacactt gcgctcgatt cttggttcta tgactgtaga
     5761 agaaatctat caaaatcgcg ataagttcag tcaaagcgta caagaagtcg cttctgtaga
     5821 ccttgcaaaa atggggttga tcatcgtttc tttcactatc aaagaggtca gagacaaaaa
     5881 cggttatctt gactctttag ggaaaccaag aatcgcccaa gtcaaaagag atgcagatat
     5941 agcagaagcg gaagctctaa aagaaacgcg tatcaaaaaa gctcaagcag aaaaagaatc
     6001 acaagcagct gaattacagc gacaaacaga gatcgcagaa tcattaaaag aaaaagaact
     6061 gaaattagca acctacaaac aagaacaaga tgttgctaaa gcaaaagcag accaagccta
     6121 caatttagaa agtgcccgag cacaacaaca agtaatcgaa caggaaatgc agattaaaat
     6181 cgtagaacgc caaaaacaaa tcgaactgga agaaaaagaa atcacacgtc gtgaaaagca
     6241 atatgattcg gaagtgaaga aaaaagcaga tgccgatcgg tacgctaaag aacaagaagc
     6301 acaagctcaa aaagtcaaag aagtgactga agccgaagca gaacgattcc gtgtggaagc
     6361 actggcagaa gcggaagcca ataagacacg tttagctgga caagcagaag ccgaagcagc
     6421 actagcaaaa ggaaaagcag aagccgaagc aaaacaaaag atcgccaatg ccttcaagga
     6481 atatggcgaa gcagctgtct tgagtatggt tatcgatatg ctaccgcaat tgatgcgcga
     6541 agcagcacag ccgctaggaa atatcgaaaa aatctctgtt gtcgatacag gaagcagttc
     6601 aggagaaacc agtggagcga accgagtgac aaattatgcc acaaatttgc tttcttctac
     6661 tcaagaaaca ctaaaagcga caacaggact tgacttgaag gaaatcgtag agaatttgtc
     6721 gacacgtggg actactagcc aagtaaatat gtatgaagcg ccaaaagagg cagaacaaaa
     6781 ctcgatgaag gaagaataaa gtagtacact tcaccgttca atcaaaaatg gaggaagact
     6841 ttttaaagtt cttctctcca tttttttatt ttattgcaaa agaaaattag tcctcgctat
     6901 ttttcttttg tttttggatc ttagcttcta tctcttgtaa atttgttctt agcaatcgtt
     6961 ctttcttttg gagttcgacc aaatcttctg atagttcttc cttcttttct gtttcaggag
     7021 gacgaagaaa gaaattgata ccgattgcaa tgaatgtccc tataaaagta tctagtacac
     7081 gttggatagc atacaaagcg gattcgcctt gagggatgct caacgcaatc agcaacaacg
     7141 tagctattgc ggaaatgata ccggaattat tgttaatacc atctgacacg acgatcacaa
     7201 taataactag aataggaagc aagatcaatt ctaccaaaaa atcattgtga aaatagcttt
     7261 tgacaaaaaa ataaatgata gctaatgtac cacctaatgt attgccgatg atcctagact
     7321 ttccaaaaga aacggttgta gtcaagtcct gacgcaaaga aaaaacagca gataatgcag
     7381 caatcatcgg gattcctcgt tgagtcaaat gaaaaaacaa gatacataaa aaaacagcta
     7441 aggcactttt gatcgtacgc atacctagtc gaaaacggcc aaattgcata gagattctcc
     7501 tttttggtct ttttactatc atacctaaac ttttgtctga tagctagttt ttcattattc
     7561 aagtataatt aaaataaaaa agggggaagt aaatgcgtat aggaatgcga acagtcaaaa
     7621 cagtgatcag tgtcttcttt tcgatggtca cagcgggtag cttatcgctg ttgtattggc
     7681 cagctgcagg gatcattgca ttattaagtg taggaaatac caagcgctct acattgcgta
     7741 cgggcatgaa tcgaatcgca gcctttatat tagctacggc aacagcactg ttcagttttt
     7801 ttgctgttgg gtatactatt tttggcttca gtcttttttt attcctgttc atcccgcttg
     7861 cctctcgctt caaattaacc gaagggatag tagtaaattc ggttttggtc actcattatt
     7921 tagtggagga aaacatgtct tggcagctga tcggcaatga agcgggatta atggcgattg
     7981 gattagtatt tgctttgctt gcaaatgttt acatgccaga tcgtacgaag cagctgaagg
     8041 aaaaccaaat acagatcgaa aaagaatttc gaaatatttt gagacaaatg gcagaatttc
     8101 ttttatcaga aaataaagaa gatgtgcaga tcaaatgcga acatttactg acattcattc
     8161 gcgaaagcca agaagatgcc agagaacatc aagaaaacta ctggctcaga cagccgctat
     8221 actacgaaac ttacttctct atgagaagag cacaggccaa tgtgattaaa gacatgctgg
     8281 aaaatctaga gagaatccag caaccagctt attatggaaa acatatctat ggtttattga
     8341 tttatactgc agaaacattt tcagagtcaa atgatggcag acaaatttta acacgtattg
     8401 aagaagttta tgtattgtat cggcaaatgc ctctgccgac tagtcgccct gaattcgagg
     8461 atcgagcaga attgttccag tttttgcagt cttttaaaag ttttattgaa atcaaggcag
     8521 aattctccca acaaaaaatc caaaaataga ggttgtgaat tcagctaata ttcaggtcaa
     8581 aaagctgatt tcacaacctc ttctttaaac ggtgttctcc tgtcaactcc tcggtcacaa
     8641 gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcggag
     8701 ctgaacggta ggctcacaac ctctttctta aacggtgttc aaaagctttc ctctgcggta
     8761 ataagtccag ccaaacgaaa aataggagga gctatttttg tttggctgtt cttattactt
     8821 gatgcagaac agctttttca caacctttta aaattacata tttttccata gaacttgcat
     8881 ttgatccaag atttcgatga catcttttgt caagaagaga gagcgattgg gcaagtcacc
     8941 ttcaatcatg tgtaccatat tctttatttc gtaattcatt gcttcagatg tattcccgct
     9001 ttcgatgaat tcctttgtac catctgtaaa taagatctcc gcacgatcag cacgcgggta
     9061 atcagcaatc gtcacataac cgttctcaaa ggcaacgata ccgacttttg gcatttttgc
     9121 ctgaaaagtc aagctgacag cagcaagttc attttctttg ttacgcaaga tagtgacgga
     9181 ttgttcgtcc acgcctgttt caaaaggaac cattgtagag gcaactactt caggttgaga
     9241 gctcaaaaag aaacgagcga aagatacagc atacgtaccg atatccaaca gagctcctcc
     9301 agctaattct ggattgaaga accggttttt agggtctggt tctttgtaac tgccgaaagg
     9361 tgcttggatc atttttaaag cccccagttt tcctgtatcc attattgagc gtaactgctg
     9421 ataaagtggc atgttgaaga ttgtcatcgc ttctgctaag atcaaattct tttcttctgc
     9481 taagcgcata gcgtcagcca gttctttttt attcatcgtg atggcttttt cacatagaac
     9541 gtgtttgcct gcttccaatg ctgccaagat atggtcgata tgctgtctat ttggtacagc
     9601 aatgtacacg atgtcgattt cagcatcgtc taacatttct tgatagcttc cgtaagcttt
     9661 tggaatattg taacggtgag cgaagttttc cgctttttct gaagttctgg aagcaactgc
     9721 tgttagttcg ctcgtctctt gattgaagct ttcggcaaac tcatgtgcga tggtaccaag
     9781 accaacgatt ccccattgat attttttcat ctgaaaacgc tccttcatag ttggattgag
     9841 tttcttgtct gtctattatt ataacaaaaa tcacgaagaa taaagaggag aggacttgaa
     9901 acattaaaaa aaatgaaagc agcgatttaa gaggtgcttt ttgatcaaaa cttaagtaga
     9961 atagttaaaa ggaggggata aacgtgcatc aagtatatat tgatcttatt atgagtgtat
    10021 tattagaaca gtttgaaact gaaaaaaatt ttttagatga atacttgctg atcagtccag
    10081 aacaatggca aaaatggaaa gatggcgaga aaaatctgcc atctgaagcg atgcagaaag
    10141 taaaaaatct tttcactgat tatgaatgga tgctaacgca aaagattttg cgtcaaacga
    10201 ttatttttcc cgaaaaacga aacgttgccg ttgcagagta caagcaaatc aaaacacgca
    10261 tcgcgcaaaa atggcttagc agcaaagcag caaaagtcga gctgatccct ttgaaaaata
    10321 atcagcaggc tggtagatac cttgacttga aagtaagtat tcaatatgat gaatggggct
    10381 atgatgatat tttgaatttt cgtctgccag cagatgttca aaaacagatc gaaggagaaa
    10441 aaatcgaact tctcgattgg gtgaatgaaa atctggaaga aacttacgtt catgaggcga
    10501 atggggaacg tgaagaacaa cgaaaggatt tttcaagtga ataaattttt aggatttgga
    10561 gcagttgtga ttatgatcgc agcaatgggc tatgttattt ttagcgatga tgattcgcat
    10621 gttcaagccg gcaatcaagc agtcgaacga acaagcacca aaacaactgc tagtaaagaa
    10681 aagaacagtc aaacaaccag cagcaaggta aaggacaaag aaacaaaaga tcgtacgtcc
    10741 aaacaattgc cagatgttaa aacagacgat tggaagctag ttttggttgg tccagatcac
    10801 aaattagaaa aagaaattga tgagtctact caattggcat cattaagtaa cggatacttg
    10861 atcgacaaac gcattgctcc agaatatgaa gcatttgcca aggctgctga agcagctgga
    10921 tttccattag tgatggtctc agcttaccgg tctgtatcat cccaacaaca agttttcgaa
    10981 caaaatgtcc aagatgtgat gagtcgtcaa ggtgtatcag aagaggaggc gacaaagatt
    11041 actaaagaaa cgattacgga gccaggatac agcgaacacc acacaggtct ggcagtcgat
    11101 gtcgtcgatc aaaactggta caacagttat ccttctactg tattggatgc aagttacgga
    11161 gatcagcctg gagctaaatg gatcgcagat aatgctgcgc agtatggatt tatcgttcgt
    11221 tatccaaaag gacacgaaga tataactaag atcacatatg aaccttggca cttacgttat
    11281 gtcggtaaag aaaacgctga gtatatcacc aaacaccaat tgacgctaga agaatatctg
    11341 aatgaattaa atgaaaagta ggaattgatc atggcgaaaa aaaagtggaa aaagcaaaag
    11401 cgcaagaata tccgttggcc agcagtggtt gccggtgtag cagtgatact cttagccttt
    11461 gttttttcct tgaagaatct gtcttctcct tttactgata atcagcaaga aaatgaagcg
    11521 caatcccatc agcaatttat tgaacggctt gttccgcatg cccaagaact acaagatggt
    11581 tatggagtgc ttcctagtat cattttagga caagctatat tagaatcaaa ttggggaaaa
    11641 agtaccttgg ctagcaaata caataatctt ttcgggatca aggcttatgg ggatcagaag
    11701 aaagtaagcc tggaaacaaa ggaatttgtt aacgaagaat ggataactat ccaaggagac
    11761 ttcaaagtgt atgattcatg ggaacagtcc atggatgacc atacacaact gttcgtccaa
    11821 ggagtagatt ggaatcctgc gctgtatgaa aaagtgatta cggccaccaa ctatcaggaa
    11881 gccgcacaag cattgcaaga tgcaggttat gcgacagacc ccggttatgc gcaaaaaatc
    11941 atccaggtca tcgagacgta tcaattgaat cagtatgacc ggagatagca aaggagaagt
    12001 ggctgatggc agaacaattt acgccagaaa tcatgacgga attgagaaaa gatatcgtaa
    12061 gagtaccgga aatcatttta gaagcaagcg ggatcaagat atttggaaaa ttgatccgtt
    12121 cgatcatctt tactacggat attgcgatca tacgtaatac aaatgcgaat gcagtaattg
    12181 ctgtgtatcc ttttacacct catccagcga ttactaaaag cattattgag gcagctgaca
    12241 ttccagtttt ctccggtgta ggtggtggtt tgacacaagg tgctcgttct tcctacatga
    12301 gcatgttcgc agaagcgcaa ggttcgttag gggtcgtttt gaacggtcct acgcctttag
    12361 agacagtaaa agaagtttgt cgagtagttg atattccagt tatcagtacg gtaacgtcga
    12421 aatatacaga aatagatgaa aagttagcag ctggtatttc gattatcaat atcagtgcag
    12481 gtaaagaaac tgcagaagca gtacgccatt ttcgtaaaaa atatcctgaa cttcctatca
    12541 ttgcgacagg tggtccaacg gaagaaagta ttgctgagac cattcaagca ggagcaaatg
    12601 ccatcacata tacgccacca agtagcggag agctttttag tcgtaaaatg gaaaaatatc
    12661 gggaaactga gaaaaagaat catgaataac aggtgagaat caaaagacga ggaagagcag
    12721 atagtggctc ttcctcgtct tttgtactcc agtactattt gttcagaatt caaaaaaaag
    12781 agaaattttt ttgttttttt acctgtaatg tacataagaa gagttaaaac cttttgttat
    12841 tattagcatt ctaacaaaaa aatgtcttga tttattgaag cgctttctat agaatgaaaa
    12901 acgaataaaa actaaaaaag gcacccgaca atttagtagc agctaaattg tcggggcttg
    12961 aaaagtcagc gggaactgac caatcaagtt gccatagtca atgcttgtgc atcgacgtgt
    13021 atattgtacc taatttcata cagaattact agttattttt ctgtttttta gggaagagcg
    13081 cacacgaaga agcatgctta tttagctatg tcaaggatat tgggcagttg tctgatatcc
    13141 ttttttcttt gcgtaattat cctcccatca ttgaagcaag aagtgttttt agcgatgaaa
    13201 gaagaatcag cactatctaa tttttcttga aagtattgag ttgttcatcc taggcagaac
    13261 gataaggcaa gtcttggttt ttattcccct tgaaaaagat cgtttaataa atatgttctg
    13321 tataaaaata cagtatattg aaaaataaca atggaagtag aggtgcagag aatgaagaaa
    13381 agaatgagaa tcatcttagc tttaagtgtt atgttattct tcttgacggc ttgtgggcaa
    13441 gaagatgaca aaaaaatcac gctgaacatg gcttggtggg gttcgcaagt ccgacacgat
    13501 gcaacagtaa aagtaatcga gctttatgaa gaaaaaaatc cccatgtgac gatcgattat
    13561 gaattttatg attatgaagg atattacaca aaattaggta cactagcagc atcagatgat
    13621 atctgggaca ttttccaact gaacaatcaa tttcctcaat acatcaatca gattgaacca
    13681 ttagatgaat atgtggagca agggataatt gatatttcta atgcagatga atcctattta
    13741 gcaacgacga cttacgacgg gcaattagtt ggactgtcta acggggtgaa ttcgtttgcg
    13801 attgcttaca ataaaaatat atttgaacaa ttaaatatcc ctgaaccagc ttcaaattgg
    13861 acatgggatg aatttgaaca aatcagctat caaatcactg aagaattagg aatctatgct
    13921 atttcaagat tcgaagattt tatagctggc tgtattattc aaataccaca agttgagaaa
    13981 ggattgaatt tttataatcg ggaagatcta tctaacagct tgggatttga aaatccagac
    14041 tatctaacag attttttcca gctgcgtaaa aatctggtag acaaaggggc ccatccagaa
    14101 ccaggtggtg gagctgcaag tacgacggat ctgtcttttc aggcggttag ggatagagaa
    14161 gcggcaatgg tgtttctatc aagtaaccag ttgacagaaa tattgtcagg ggctcctgaa
    14221 gatatggaga ttcagttgat taatccacct cgcagaaaag cagatggaga atctggtatc
    14281 cctttacgtt cttcgcaaat gctgagtatg gcaaaaagct cagcccataa agaagaagct
    14341 gctaagttta ttgatttctt tcaaaacagt gaagaagcca atgaaatttt aaaaggagaa
    14401 cgtggtgtac caatcatgtc gaatattcga gaacagctcg ctcgtgcaga tgatgcaact
    14461 ttgcaagcga catttgatta tttagaaatg atttccgata tggacaacgg cgaagtgaat
    14521 gtctttgata gtgctttgaa tccggaaatt caagaccaat acaatctttt agttgaaaaa
    14581 gtcatttatg aagaagtaac agcagaagga aggcatgaaa aaagaaaaac taaaacggtt
    14641 cttggatcaa gacaatgtgg tcggttacgt atttgcttct ccattcatcc taggatttct
    14701 attgataaca gtgataccga tgatcttgtc actttattat tcgttcaccg attatcgttt
    14761 gggaaatcca gtctcatgga tcggcttgga taattatata aagatgctga gtgatgcccg
    14821 ttttttgaac tccatccgag tcacttttct ctatgtattt gcatctgtac ctgtgaaact
    14881 gatctttggt ctattgattg cttatctttt aacgaggaat atcaaaggaa cgactttctt
    14941 tcgttctttg tattatgtgc ctagtttgat cggtggcagt atttctatcg ctctagtttg
    15001 gcgggagttg ttttctaaaa acgggttagt gaatattttc ctggaacgat taggcgcacc
    15061 tgcgctttcc tggtttagcg atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc
    15121 ttggcaattc ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga tcccaggctc
    15181 gtattatgaa gcggcagaga tcgatggagc gaacaaatgg cagacgtttt acaagatcac
    15241 gttacctctt ctttcgccga tcattttgta taacttggtt atgcagacaa tcgcggcgtt
    15301 tatgactttt acgcaggcat ttatcattac ccaaggtggt ccgaatgatg ccacgaactt
    15361 ttatgctctt tatgtgtata accaagcttt ttcttttaat tctatggggt atgctagtgc
    15421 tatttcgtgg atcatgctgt tgattatggg agctgtcacc tttctgattt tcaaatcatc
    15481 aaaaaaatgg gtcgttgtgt gaatgagagg aggaagaaat gaataataca gtaacgaaat
    15541 ggtttcaata cggttttatt ttgctattag gtctagtcat gatttatccg gtcttatgga
    15601 tgatcgcagg ttcttttaaa acggatcaag agattatgag tggttcgctg aatttgattc
    15661 cagagacttt tcaatgggaa aattacgcaa aaggatgggc ggggtttgct ggaatttcat
    15721 tttttacttt ctttaggaat tcgtttgtca tctctgcagt ttcgacgata gggacagttc
    15781 tgagctcgac ttgtattgct tacgcattat cgagaatcga tttcaaagga aaaagattct
    15841 ggttcgtcgt catgttagta acgatgatga ttcctgcaca agtgattttg attccgcagt
    15901 ttattattta caatcggctg aatttggttg ggacgtatgt gccgctgatt ttaccacatt
    15961 tttttggaca agcatttttc atttaccaga tcatgcaatt catggtcagc atccccaaag
    16021 agttagatga atcagcgatt attgacggat gcagcaagta tagtgtcttt accaaaatta
    16081 tttttccttt gctgaaacct tcgatcatca cgacgatcat catccagttt tattggaaat
    16141 gggacgactt catgggacca ttgatttatt taaacaagcc gagatcttac acggtatcga
    16201 ttgccatcaa attatttgca gacgctggat ctagtacgac ttacgcgagt atgtttgcga
    16261 tgtctacgtt gtcgctgcta ccggtttttc tgattttctt attcttcaat aaatatcttg
    16321 tccaggggat cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa tagaagagat
    16381 gtttctttac aagctcaact actatgtctg tcggattttt ttaaccagtc ttatcagtaa
    16441 tgctcttttc gctttagttc taggatgttt gttcacttta ggattagtgc tgtctgctcg
    16501 tgttgtctat ctgatttatt ttttgatgat gtatccttct tattatctat ctgtccatta
    16561 cttggcagaa aaaaaggtat gttggaggaa agggaaaatc aaacggatgt gtttcatcat
    16621 tacgttgttt cagctgtttg tcattatttt tgcagttgat tggtatttat tagtgggaaa
    16681 gattggagct tggtatctga ttccttttct attgtgtctg ttgctttttg tatcccatag
    16741 tttttttagt acgctaattg atatctgcaa agaagataaa gcagatagtc ttgtttattt
    16801 gatcaaaagg aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg
    16861 ccttttgtat ggactgttct ggtgtataca gcctgatttt ttactcactg cattactgta
    16921 tccagggctg ccatttgtca cagtattttg cttaaaagaa aaggagaatg actttaaatg
    16981 aagaaaaaat atgtacaagt cggacttggt gggagagcgc gtttttttta tgaggcaatt
    17041 gcctctacgt atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg
    17101 atggattatg caaatcggat acttgtgaac aagtatggac agaaaaaagt aaagacatgt
    17161 atgccatcac aatttgagca actactggat gacgtacggc cggctgaagt cattattacg
    17221 tcagtagatc gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt
    17281 atttgcgaaa aacctctgac tatcgatgag gaaaaagctc aagaaataat aacggtccaa
    17341 aaacagacgg gaagaagcct gcgagtcact tttaattatc gttatgcacc gtatcacacg
    17401 aagataaagg aactgataga aagtgatgtg attggtgatg tgttttctat ccacttcgaa
    17461 tggctgttaa atacgcaaca cggggcagat tatttcagaa gatggcatcg aaataagcac
    17521 aatagcggcg ggcttcttgt tcataaatct acccatcatt ttgatctagt gaatttttgg
    17581 ttgaacagcc gtccccaaaa ggtatttgca ttcggtggat taaactttta tgggatgcat
    17641 aatgcggaga aacgcggagt agaaatattt tacagtcgat gctatggaag cgaactagct
    17701 aaagctgatc catttgctat agacctgtca gccaatgagg aattgcgaga aatgtattta
    17761 gaagcagaaa aagacagtgg atatattcgt gaccgcagtg tattttccga tgatatctct
    17821 attgaagata cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg
    17881 ctaaacgcgt atatgccgtg ggaaggattc aatgtttctt tcaatggcag caaaggacgg
    17941 atcgaaatga aagtgatcga aaagtcttac atcaatgcag gtggagataa aaaggatgaa
    18001 ggagcagccg ttcttcagtc catccatgtc tatccgatgt tcggtgagcc atatgaggta
    18061 atcgtcgaac aagaagcggg cggacatggc ggaggagatc cgcgtttatt agatgatcta
    18121 tttggtgaag aaaaagaaga agatccgtgg aatcgggcag caactcacgt agatggtatt
    18181 ttgtcgattt tgacaggaat cgcagcaaat cattctattg cttcaggaaa agcagtagtg
    18241 attgacgagc ttgtcagctt aaaagaataa gaatagattt acggaaatta cttcccgtag
    18301 tttataaaat cttgccttat tgccgatagg ctgtcttttg aacactattt attcggaaaa
    18361 aggggctatg acatgctttt tgtcatgcct tctttttatg tgggctgctt ttttagatag
    18421 gagaaaataa aaataactat ttaaacccga acaaaaagga ataatgcatt aaaaatcatt
    18481 agattttaca aaaaaataag taaaccacgt tgtcagtttc agaaagtagt gttagactgt
    18541 tttcaatatt tgaaacaaca ttttttattc aaatatggca aactgccttc acgtgcaaaa
    18601 cagttgcaag aaaatgaatt tacactatta ttagttttac atacctactt atatatcgtc
    18661 ataatatgga tgacagtttc tagccagtac cgtaaatgct ggactataag taaaagattg
    18721 gctatttcac ccttcttttc ttatgataaa gtggagggtg tttttcttta acagaaacat
    18781 agtaggtttt tgcacaaaag cggttatttc ggtattaata aatgataaag gagcaaataa
    18841 agtgaaagaa ttagttgaac gtattcaaaa agacggacgt atattaggcg agggcgtatt
    18901 aaaagtcgac agcttcgtca ctcaccaagt agaccctgta ttgatggaac aaatgggaaa
    18961 acggtttgca gaagtattta aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc
    19021 tgggatcgca ccagctttat ttgctgcaag agaattagat gttccaatga tcttcgctag
    19081 aaaagcaaaa agtttgacga tggatgaaga attattgaca gcttctgttt attcttttac
    19141 gaaacaagtg acaagtacga tttctatttc tcgtaaattt ttatcttcag aagacaaagt
    19201 attgattatc gacgatttct tggcaaacgg acaagcggca aaaggattga tcgaattatg
    19261 ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga
    19321 tggacgtcag ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc
    19381 cttgacgaat ggacaagttg agtttcttga ggaggacgca taatcgtgga aaaagaaaca
    19441 caaaatggaa aagctgcggt tttagggctg cagcatctgc tagcgatgta tgcaggagca
    19501 gtagcggtgc ctcttttgat cggtacagga ctaggcttta atcaagaaca gatgacttat
    19561 ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat tattgcaatt gacagttaac
    19621 cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca
    19681 ctgatcttga tcggaaccaa tgaaggagta ggagctatct acggatctat tatcgcttct
    19741 gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact attccctcct
    19801 atagtaacag gaacggttat caccgttatt ggtttgactt tgattcctgt agcaatcgag
    19861 aaaatgggtg gaggtaatgc aacagcagct gattttggtg acaaaacaaa tctgctactt
    19921 gcatttgtga cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt
    19981 tcaatcgcgg tattgattgg tttagtcgga ggaagcatct tagctgcttt catgggaatg
    20041 attgatctga gcgcgattgg agatgcgccg ttgttccatg ttccacaacc cttttatttt
    20101 ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga tcatcatttc catggtcagc
    20161 atggtagaat caacaggtgt atattttgca cttggtgaca ttactgggaa aaatgttgga
    20221 gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt aggcggtatt
    20281 ttcaatacat tcccttatac tggcttttct caaaatgtcg gattggttca actttcggga
    20341 atcaaaacaa gaaaacccat ctatttctct gcattcttct tgattgtttt aggattattc
    20401 cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg tacttggtgg aggaatgtta
    20461 gtcatgttcg ggatggtggc agtccaagga atccgtatgc tgttggaagt tgatttcagc
    20521 aatgataaaa atctattgat tgttgctgtt tctgttggat ttgggctggg tttcaatgtt
    20581 acgccgacat tattcagtca aatgccgcaa acattgcaaa tgttcactgg aaatggcatc
    20641 gtaatgagta gcattacggc tatcatattg aatttgatct ttaatggatt gaaaaaagat
    20701 gaagaaatat tggaagaagc cttcaagaag taatagagca aaagaaaggt tgtgaaatca
    20761 gcgttttgac ctgattgtta gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt
    20821 tgtttgagct aataccgagg gtcagctgat tgaacaccgt ttatccataa aaaggaatgt
    20881 gacctaactt ttgtcacatt cctttttttc atctttaata aacacgaaca ataaaatgtt
    20941 tttctttgtt aacattcgaa atttctttga catcaaaaaa agtgattgtt aaaatatgaa
    21001 tgtagaaaaa ctgggaggaa aaacaatgac agcaaaaatc tctgtgatta tggggagtac
    21061 ttcagattgg gaaacaatga aagaagcatg tcaaatattg gatgaattcg gtgtgtctta
    21121 tgaaaaaaag gtcgtttctg ctcatcgaac cccagatttg atgtttgaat atgcacaaaa
    21181 cgctcgaaaa aaaggaatca aaatcattat tgcaggagca gggggagccg cgcatctgcc
    21241 gggtatggtc gcatcaaaaa caactttgcc ggttatcggt gtaccggtcc aatcacgaac
    21301 attaaatggt ttggattcgc ttctttcgat tgtccaaatg cctggaggag tacctgtagc
    21361 tactacagca atcggaaaag cgggagcaat taatgctgga ctacttgcga tacagatgct
    21421 ttccatgtat gatttagaat tggaggctaa attggctgaa agacgttcaa tgttggctaa
    21481 aactgttata gaaagtagtg atcaacttgg ttaaaccttt gttgcctggt tcaacgatcg
    21541 ggatcgtcgg tggcggtcaa ttaggtcgta tgatggcgat tagtgcaaaa gaaatgggct
    21601 ttaaagtagg tgtgctggat cctgtgagcg attgtccagc cgcacaagtt gcagattggc
    21661 acattgaaag tgtctatgat gacacctttg cgctgaaaga gcttgccaga agaacagatg
    21721 tcattaccta tgaattcgaa aacgtcagtg tagaggcgtt aaatgcgatt ttacccatgt
    21781 catttattcc tcaagggaca gacttgcttg cgattacgca agaccgcctg ttagaaaaat
    21841 cttttttgga aacgaataac atcgtgattg ctccttatgc aacgatcgtc agtccgacgg
    21901 atatccaaga tgcgattgac gggatcggct atccttgtgt tttgaaaacc actcgaggcg
    21961 gctatgatgg caaggggcag tatgttttga aaagtcgggc ggatctagct cctgccatgg
    22021 accttttaag agaaggaacc tgtgttttag aagcatggat cccgtttgaa aaagagattt
    22081 cgatcatggt ggcaggaaat ggtcaaggag atttcacgac ttttccggtc gtggaaaaca
    22141 tccaccacaa caatattctt catgaaacga ttgctccagc cgcgattgat caggatgtga
    22201 tcgaggaggc tgagcgtatt gcccgtgtga ttgcagaagc agtgagcctt tcaggtgttt
    22261 taggggtaga aatgttttta acaaaaacag gcgggctata tgtgaatgaa ttagctcctc
    22321 gtcctcacaa ttctggacat tattctattg aagcttgttc tatgagtcag ttcgatgccc
    22381 atattcgagg tatttgtggt tggccattag gagaaaaagt acgtttgata agcaatgcag
    22441 taatggtcaa tgttttggga gaacaactgc tggaaagcta tcgcttgatt gagaataagc
    22501 cagactggca attccatttc tatggaaaaa aacaagcaaa aaaaggtcgc aagatgggac
    22561 atatcactat cttgacagaa gacatccagc aaacgctgga agaaatcaaa caaacgaata
    22621 tttgggatta gaggagaaaa aacatgttag aacgttatac acgccctgaa atggggaata
    22681 tctggacgga agagaacaaa taccgtgcgt ggctcgaagt cgagatttta gcagatgaag
    22741 cttgggcaga acttggtgaa atcccgcaag aagatgtgaa aaaaattcgt gagcatgcat
    22801 catttgatat cgatcggatc ttggaaatcg aaaaattaac acgccatgat gtcgttgcgt
    22861 ttacacgagc ggtttctgaa acattgggag aagaaagaaa atgggtccat tatggtctaa
    22921 caagtacgga tgtcgtagat actgcttatg gctatctcat caagcaagca aatgatattt
    22981 tacgggaaga cttgaaacgt tttacggaaa tcattggcga aaaagcaaaa gaacacaaat
    23041 atacagtgat gatgggtcgg acacacggtg tccatgctga accaactact tttggattga
    23101 aacttgccac atggtattca gaaatgaaac ggaacatcga acgatttgaa catgcggcaa
    23161 aaggagtaga agcaggaaaa atctcaggag cagtgggaac gttcgcaaat atcccgccat
    23221 ttattgaaga gtatgtatgt aaacaattgg ggatccgtcc acaagaaatc tctactcaag
    23281 ttctccctcg ggatctacat gctgaatatt tctcagcgat ggcgcttatt gctacaagta
    23341 ttgaacgttt tgcaacagag atacgcggat tgcaaaaatc cgaaacacgt gaagtagaag
    23401 aattttttgc gaaaggtcaa aaaggttctt ctgctatgcc gcacaaaagg aatccgatcg
    23461 gttcagaaaa tatggctggt ctagccagag tggtgcgcgg acacatggtc actgcctatg
    23521 aaaatgtcac attatggcat gaaagagata tttctcattc ttcagcagaa cgaatcatct
    23581 tgcctgatac cacgatttta ttgaattaca tgttaaatcg ttttggcaat atcgttaaaa
    23641 atctaacggt ctttcctgaa aacatgaagc ggaatatgtc tgcaacattt ggtttgattt
    23701 atagccagcg cgttatgctg aaattgatcg ataagggaat gacaagagaa gaagcttatg
    23761 atcttgttca gccaaaaact gcgtacgctt gggacaacca agtgcagttc cgcccgctgt
    23821 tagaagaaga tccaaccatc acatcggtat tatctaaaga agaaatagac gatgcctttg
    23881 attatcatca tcatttgaaa aacgttgacg tgatattcaa acgagtaggt ttggaataaa
    23941 gaagagtgag ttgcaaaatt caattaaaat agaatagcaa aaataaccaa aaaagaagca
    24001 gatccgttag cggttctgct tcttttagtt tattatattt tcttatcaat aataggaacc
    24061 tgattttaaa agcggctacc tctttaccgt gcctctttac atacaaaaaa tagaggaatt
    24121 aagcgtaaat ggcttggcta ttataactaa aatgctggtt atggagatgt tgcagcaagt
    24181 tttcgagaaa agcgtattcc atctcttgtc cattgaataa gatctggata cgataatgat
    24241 ttttgaaacg cttctgttca tgaatctgac tgattgaaag ttcgcctttg tgttctgtct
    24301 gataaagaaa agtcctttta cggcattcag cacagcgctt ggttgctcgg cggatttttc
    24361 ggaatgtata aaaactagta tctttattgc ataaataaac gtctttgatc gacagcaaat
    24421 cattatatag acaggaacgt tcttcagctg ttaattctga taaaaaaagc tgaccttgtt
    24481 ttattgcatc ttccatcggc aagtgcctcc tcgaacccat tatccaaatt tttagttaat
    24541 tctaatatac catcattaag tataaatgta aacgcttgtt gatggtataa gattttcgtt
    24601 tctgataaaa cacgaacatt aaaagaatct aatgccttaa atgtacatat ttttgttgat
    24661 ttataaaaag atgattgcta aaatttaaag tgaaaaaacg aacaaaaaac gatgtatttg
    24721 cttttaatgt aaataaaaag gagagacgta catggagaag aaagaacttt tgtatgaagg
    24781 aaaagccaaa aaaatatatc ttacttctga tgagaacttg atttggatag aatatttaga
    24841 tcaagcaact gcgttgaatg gcgcgaggaa agaccagata agtggaaaag gagaattgaa
    24901 taatcaaatc acaagtgcaa ttttccgtta tctgactcaa agaggaataa aaaatcattt
    24961 tattgaacaa ttgtctaaaa ccgaacaatt agcaaaaaaa gtacaaatta tccctctgga
    25021 agtggttatc cgaaatattg cagcaggaag tttctcaaaa cgtctaggtg ttcccgaagg
    25081 taaagaattc atcaaaccaa tcattgaatt ctactataag gatgaccagt tggatgatcc
    25141 atttatcaat gaagagcata tctatttctt acaacttgca aaaccggaag aaatcagtga
    25201 gctgaaagcc caagcgttgg ctattaatga aattttgacc gaattatttg ctaaggtcgg
    25261 tttggtttta gtagatttca agctagaatt tggccggtcg aatggacaaa ttttgcttgc
    25321 ggatgaagta acaccggata cttgccgatt atgggatgaa cgtacgaatg aacatatgga
    25381 caaagacgtt taccgcagag atctaggtga tttgatccca gtctatcaag aagtatataa
    25441 ccgtctatcg gaactaactg aagaaaagga gcgagtatga tgtatttcgt aaaagtatat
    25501 gtaacctata aagattcagt gttagatcct caaggggagg cagtaaaaaa agcagtccat
    25561 cgtatgggat atgaaacaat cgaagatatc cgaataggaa aatactttga gatacaactt
    25621 gctgatgcag aagaaccaat cgatacagtg attgaaacga tttgtgacaa gctgcttgca
    25681 aatgtaaata tggaaacata ccgatatgaa atcaaaaaaa tggaggaagt ataacatgaa
    25741 atttgcggtc atcgtttttc caggttcaaa ttgtgacgca gatatgttat gggcagttcg
    25801 cgagatcatg ggcgctgatg ccgaatttgt acggcatgat gagtcctctt tagaaggatt
    25861 tgatggggtt ttactgccgg gaggattctc ctatggtgac tatttgagat gcggtgcgat
    25921 tgctcggttt tctacaatca tggaagaagt gattcgtttt gctgaagaag ggaaaccagt
    25981 attcggtaca tgcaatggat tccagatatt gacagaagca ggacttttgc caggtgtact
    26041 gagacgaaat gagtctttgc attttatttg caaaactgta ccattgaaag tcaaccccaa
    26101 gacggccttt acttcagagt atgaagccga cgaaataatc tatcttccag ttgctcatgg
    26161 ggaaggaaat tatttctgtg acgaagaaac acttcaagaa ctaaaaaaaa ataatcagat
    26221 tgcctttact tataatgaca acatcaacgg cagcgtggaa aacattgcag ggattacgaa
    26281 caaagcaggg aatgtgttag gaatgatgcc tcatccagaa cgtgcagtag aagacttgct
    26341 aggatcagaa gatgggaaac ggttctttgc ttccatttta aaaaacttcg gaaaggtgac
    26401 cgtatagatg aaaacattga aagaaccgac agctgaagag atcaagaaaa aaagaatcta
    26461 tgccgaatgg ggattgactg atgaagaata tcgaatgatc gaagaagata tcttaggaag
    26521 aatgcctaat tatacggaaa ctggactgtt ttctgttatg tggagcgaac attgttccta
    26581 taaaaattct aaaccggttc tacgcaaatt tcctactagt ggacctcatg tgttgcaagg
    26641 ccctggagaa ggcgcaggca tcgtcgacat cggtgatgga ctagcagttg tcttcaaagc
    26701 agaaagtcac aatcatcctt cagccgtgga gccttatgaa ggagctgcga caggagttgg
    26761 cggaatcatc agagatattt tcagcatggg ggctcgtcca attgcgttgc tagattcatt
    26821 gaggttcggc gaattagata atccaagaac gaaatattta ctagaagaag tagttgcagg
    26881 tatcggcgga tacggcaatt gtatcggcat accgactgta ggcggcgaaa ttgcatttga
    26941 tccttgttat gaaggaaacc cattagtcaa tgcaatgtgc gtaggtttga ttgaacacaa
    27001 acatatccag aaagggcaag ccgctggcgt cggtaattcg atcatgtatg taggtgcaaa
    27061 aacaggtcgc gacggtatcc acggagcaac ttttgcctca gaagaattta atcaagaaga
    27121 agaacagcaa cgttctgctg tacaagtagg cgatcctttt atggaaaagc tgctgcttga
    27181 agcgtgtttg gaattaatct tgcatcattc ggattctcta ataggtatac aggatatggg
    27241 agcagcagga cttgtttcat ctagtgcgga aatggcttca aaagctggct caggacttat
    27301 attgacatta gatgaagtcc cacaaagaga aactgggatg acaccttatg aaatgatgtt
    27361 atcggaatca caagaacgga tgttgatttg tgttaaatca ggagaggaag aaaaaatcca
    27421 agaactattt cagaagtatg atctagatgc agtcactatc ggtaaagtaa cagacgacgg
    27481 gcaatatcgg ctatatcatc aaggagaaga agtagcaaat ctgccagtcg atgcattagc
    27541 tgaagatgca ccagtttatc ataaagaaat gaaagaacca gctcgaatcg tagaatttca
    27601 gcagttagct ccttatcagc cagttatcga agaacctgga caagtattgc ttgatctgct
    27661 gcagcaaccg acgattgctt caaaacgttc aatctacgaa acttatgatt cacaagtaca
    27721 aaccaataca gttgtccgtc caggaagcga tgctgctgtg atgagggtta gagggacgaa
    27781 taaagcgtta gctatgacca cagactgcaa cgcgcgctat ctgtatttgg atccaaaaat
    27841 cggaggacaa atagcagtgg cagaagctgc tagaaatatt attgctagcg gtggtaagcc
    27901 cttggcgatc accgactgcc tgaactatgg ttctcctgat aaaccagaag ttttttggga
    27961 attatcgact tcagctgacg gaattgctgc tgcgtgtgaa acgttaggaa ctcctgttat
    28021 ttcaggaaat gtctctttat ataatgaaac ggatggtcaa gcaatctatc caacaccaat
    28081 gattggtatg gtcggattga ttgaggatca taaacatatc actactcagg aattcaaaaa
    28141 atcaggggat ctaatctata tattaggtaa gacgtttgca gattttgatg gcagtgaatt
    28201 acaaaagatg cagcttggaa gaatcgaagg tgtgatccga aactttgatc tatccattga
    28261 aaagagaaac caagagttag tattgacagc catccaaaac ggattgattg aaagtgctca
    28321 tgactgttca gaaggtggct tggcaattgc actggcagaa tcagctttca aacatcagct
    28381 agggatatct gtacagtttg aattgtcctc tgctcaatta tttgcagaaa ctcaatcaag
    28441 atttgtgttg acagtcgcac cggaaaataa gacacgattc gaagaaatga tgggagatgc
    28501 tgctgtttta gcaggtaagg tgacggatga agcgataatc gaaatttcag caactgacgg
    28561 tcagatcaaa atagaaactg cagtagcaag aaagtgctgg gaggatgcaa tcgtatgtct
    28621 tatgaagtga agagtttaaa tgaagaatgc gggatcttcg gtgtttgggg tcatccagac
    28681 gccgctagtg tggcatactt cgggctccat agtcttcagc atcgaggaca agaaggtgca
    28741 ggtatcgtgt ccaatgatcg aggaaagctt aaggggcatc gagatttggg gctgctttct
    28801 gaggtattca aagatcaaag aaaattgcag cagttaacag gagaagctgc aatcggacat
    28861 gtacgttatg caacagcagg caatggaagt gtggacaata ttcagccttt tctattcaaa
    28921 tttcatgatc agtcgatcgg cttagcgcat aatggtaact tgatcaatgc tcgttccctt
    28981 agaaaagagt tagaggaaaa tggggcgatc ttccattcta actccgatac ggagatattg
    29041 atgcatttga tccgacgaag tgaagaaaca gattttttaa ataaattaaa aagtgctttg
    29101 aatgaagtca aaggtggctt tgcctattta ttaatgacag aaacggcaat gattgccgct
    29161 ttggacccaa atggttttcg tccattagca atcggacaaa tgaaaaacgg agcatacgtg
    29221 attgcctcgg aaacttgtgc attagaagtt attggggcac gttttataag agatgtccag
    29281 cctggtgaga tagtgatcat caatgacgaa ggaatccaga ttgattgctt tacgaaggac
    29341 acgcagcttt ctatttgttc gatggaatat atctattttg ccagaccaga ttcaaacatc
    29401 gcagggatca atgtgcatac tgcacggaaa aatatgggac ggaatttagc gaaagaatcg
    29461 ccagtaaaag cagatatggt cgttggcgta cctaattctt ctttgtcagc cgcaagcggt
    29521 tatgcggaag ctagtggaat cccttatgaa atcggattag taaaaaatca atacattgcc
    29581 cgaacattta tccagccaac gcctgagcta agagagcaag gtgttcgaat gaaattatct
    29641 gctgtacgag gagtagtgga aggtaaaaag gtgattatgg tcgatgattc aattgttcgg
    29701 ggtacgacaa gcagacggat cgtccatctt ttgaaagaag caggggcaaa agaagtccat
    29761 gtgaggattg cttctcctcc tttgaaatat ccatgttttt acgggattga tattcaaacg
    29821 cgtaaagaac tcattgcggc aaatcacacg attgatgaaa tcaaagaatg tatcggtgca
    29881 gattctttag gttttttaag tgaagagggt ctgatcgaat cgatcggtct ggaaaaagat
    29941 gctccgtatt caggattgtg tatggcttat ttcaacggag attatccgac gccgttatat
    30001 gactatgaag aaaaatatca ggaatcacta aaagaaaaag tatcgttctt ttaaaagcag
    30061 accaagaagg gagtatatga attcaatggc aaatgcatac gcaaaagctg gcgtagatgt
    30121 cgaagctgga tatgaagtag tagaacggat caagaagcat gtcaaaaaaa cggaacgttt
    30181 aggtatgatg ggcgctatag gaggattcgg cggttgtttt gatttatcgg tctttcaatt
    30241 aaaagagcca gtccttgtct cggggaccga tggcgtgggg acgaaattag cagtggcaat
    30301 cgaagcagac aaacacgata caatcgggat cgactgcgta gcgatgtgtg tcaatgatat
    30361 cttagcacaa ggcgcagagc cgttatattt cttagactac ttagcaacgg ggaaaaatga
    30421 tccgttacgt ttggaaaatg tagtaaaagg tgtagcagat ggatgtgttc aaggaaatat
    30481 ggcgctgatt ggcggagaaa cagctgaaat gcctggtatg tactcaggta atgattatga
    30541 tctagccgga tttgctgtag ggatagctga aaaatctcaa ttattgacag gagaaacgat
    30601 caacccagga gatgtgttgc ttggaatagc atctagcggg attcattcaa atgggtactc
    30661 tcttgttcgc aaaattttct ttgagcagca gcagttttct ttatcatcaa aactgccaga
    30721 attgaaaaaa acacttggcg aagaattgct tgagccaacg aaaatctatg tttcttcctt
    30781 gcttccgctg atcaaagaaa agaaaataaa aggagctgca catatcactg gtggagggtt
    30841 tgtagaaaat atcccgcgaa tgttaccaga atctatggct gcacaaatca aacttggctc
    30901 atggccgatt cttccaatat tcggctgttt acaaaaatat ggtgaaattc ctgagatgga
    30961 gatgtatgaa atcttcaata tgggcatcgg tatggtcctg gcagtcgaag aatcgaaagt
    31021 aaaagaaata gtacacgaaa ttgagcaagc tggcgaaaaa gtttatgaaa tcgggaaagt
    31081 gatcgccaaa gaaaaacatc gtgtggtgtt tataggagaa gaacgatgag aatcgctgtt
    31141 tttgcttctg gaaacggaag caactttcaa gcgctagccg attatctatc gaaaaaagga
    31201 ttggaatctt cgattgactg gttgttttgt gatcagcctg aagcatatgt tttgaaacgt
    31261 gcaactgcgt tgagtgtacc ggcagattgt ttttcaccta aagaatttga ttcaaaaaaa
    31321 gagtatgaag aagcaatttt gcataaattg aaagaaaaaa agatcgattt gatcgtatta
    31381 gctggatata tgcgaatcat tggaccggta ttgctgaaaa attatgataa aagaatcatc
    31441 aatatccatc cgtctctttt acctgccttc cctggtttgc atggcatacg tgatgccttt
    31501 gaagctggag taaaagaaac gggtgtcaca attcattata tcgaccaagg tgtagatact
    31561 ggaccaatca tccggcagga aaaagtgaga attgaacagg aggacacgtt cgattcttta
    31621 gaagaaaaaa tccatagagt agagcatcga atctatccag aagtgattag tgaaattatt
    31681 gaaaatggag gctatctgaa atgacaagag cattgatcag tgtatcagat aaaactggga
    31741 ttgtagcatt cgcccaaggt ttgattgaac aaggagtaga aatcatttca acaggaggaa
    31801 caaagcatgc gttggataaa gctgggatca aaacgctttc gatcgaagaa gtgacagggt
    31861 ttcctgaaat gatggatggc cgtgtcaaaa cacttcatcc taaaatccat ggtggtcttt
    31921 taggaagacg cgatctccct agccatatgg aggcaatgga aacggaaaat atccaaccaa
    31981 tcgactttgt ttgtgtcaat ttatatcctt tcaaagaaac gattatgaaa ccagatgtcg
    32041 aaatagcaga agctattgaa aacattgata tcggtggtcc tagtatgctc cgtagtgcag
    32101 caaaaaacca tgaatttgtc acctctgttg ttgatccatc tgactatgaa gaagtacttt
    32161 cagaattacg tcagtatggg catacttcac tttctttcag acaaaaacta gcggcaaaag
    32221 tctttcgtca tactgcggca tatgatgcac tgatcgctca atacttaacg gattgggtgg
    32281 aagaaaaaga accggaaaaa ttgacactta cgtatgaacg aaaacaaacg cttcgttacg
    32341 gtgaaaatag ccatcaagcc gctgcctttt atcagtccgc tcttctggaa aaatattcga
    32401 tcgcagcagc aaaacaactc catgggaaag aactttctta taataatatc cgagatgcag
    32461 atgctgcgct acggatcatg aaggaatatg acgagccaac ggtagtcgcc ttgaaacaca
    32521 tgaatccttg tggtattggg acagcatcta cgattttaga agcgtgggaa gcagcgtatg
    32581 aagcagatcc tgtttcaatc tttggtggga tcatcgtttt gaatcgagaa gtagatctgc
    32641 caacagcaga acagatgcat aagctgtttc tagaaatcat cattgcccct tcttatacac
    32701 aagaagcttt ggaattactg caaaaaaaga aaaacatccg attactgaca gtagactttg
    32761 cgcaggaaga aaatcaaaat cgagaagaaa cggtctcagt cttaggtgga cttcttgtac
    32821 aagatcaaga tctggcaata gaaaatgaag aagagtggaa agtcgtgaca aaacgtaagc
    32881 cgactccagc agaagcaaaa gcattagcgt ttgcttggag agctgtcaaa catgtgaaat
    32941 ctaatgcgat cgtactggca aacgaacgtc agacagtagg aatcggagcc gggcagatga
    33001 atcgagtcgg ttcagtaaaa attgcgattg aacaagcgca agagaaaatg gaaggtgcag
    33061 ttttagctag cgatgcttat tttccaatgg atgattcggt tgaatatgca gccaaacatg
    33121 gtataaaagc aatcattcag ccaggaggaa gcatcaaaga ccaagattct attgatatgg
    33181 cgaataaata cgatctagca atggtcttta ctgatgttcg gcattttaga cattagagag
    33241 gagtcggata aatgaacatt ttggtgatcg gtgcaggtgg tcgtgaacat gcaataagta
    33301 aaaaactttt agccagtccg aaggtggata cggtgtattg tgcgccagga aatccgggga
    33361 tgacaaaaga tggtatcaat tgtgtcgcta tcagtgagcg agatcattca gcgttaatcc
    33421 attttgttaa gaaagaggct attgattgga gtatcgttgg tcctgaagtt cctttgttaa
    33481 atgggatcgt cgatgatttt atggcggaag gattgaaaat cttcggtccg acaaaagcag
    33541 cagctctgat cgaaggttcg aaaagttttg ctaaacaatt aatggatact tatcaaatcc
    33601 cgacagctaa atccagaagt tttgaatgtt acgaaaaagc agcagcgtat atcaaagaaa
    33661 atggtgcgcc aatcgtagtg aaagcggacg gcttagcagc tgggaaaggt gtagtcgtgg
    33721 ctaccacagt gaaggaagcg ctggatgcgg cagaacaaat gttgaaccag catcgatttg
    33781 gggatagtag caaaaaaatc gtcgtagaag agtttttggc aggagaagaa ttctcccttt
    33841 tggcgtttgt ccgtaatacc aaggtgtatc cgatggtcat ttcgcaagat cataagcggg
    33901 catttgataa cgataaaggg ccaaatacag gcggaatggg tgcttattca ccagtaccgc
    33961 aaatttcaaa aaagatgatt gaaaaagctg tggaagaagt tttaaaacca gcggctttgg
    34021 gaatgaaaga actaggacgc tctttcacgg gtgttttata tgcgggtttg attgctacga
    34081 aggaaggacc aaaagtgatc gaattcaatg cgcgtttcgg tgatcctgaa acgcaagtcg
    34141 ttctttctcg gttgaagagt gactttgcgc aagtaatcga tgatttatta gagaatcgta
    34201 cagtagagtt aaagtggcag caagaaggat acaatgtagg cgttgtggtt gcagctgccg
    34261 gttaccccga agaatatgag acaggaatgg ttctaccaga ttttctggaa gaagagcttt
    34321 ctgtttacta tgctggagtt tcctcaaata acggcaaact agttggagca ggaggacgtc
    34381 tttatttagt agaagcatac ggtgagacga tcaaagaagc acagaaaaaa atttacaccg
    34441 cgttaatgga taaaacgaca gatggaacat tttatcgtac agatattgga gaaaaagctg
    34501 ccaatagcga actgatcgtg aagtaaatca gctgcttcca tttataaaaa atacaacaaa
    34561 cataatcgga agcgccgatt ttagtgaagc cttttcataa agagtgtggt acacttttgt
    34621 tgtgtatata aataaggagg agttcagatg aaaaaaattc aattaggtac ttctgatcag
    34681 aaagcagctg cagtgatctt aggctgtatg cgtatcaaca atgcggaaga tccagtaaaa
    34741 gtgattgaga cagcttatga aaacggcatt gatttttttg accatgcaga tatttatgga
    34801 aacggtgaat gtgaagaaat ctttgcagat gctctagcaa aaacatcaat caaacgagaa
    34861 gatcttttta ttcaaacaaa atgtggaatc gttccaggtg tgatgtttga tttttctaaa
    34921 aaacatatca ttgaatctgt agaaaaaagt ttgacccgtc tgaaaatgga ttatgtggat
    34981 gctttacttc ttcatcgtcc agatacattg gtagaaccag aggaagtagc tgaagcgttt
    35041 gatgaattgg aacggtcagg aaaagttcgt tattttggcg tcagcaatca aaaaccaatg
    35101 caaatcgaat tgctgaaaaa atatgttcgt caaccattag tagctaatca gctgcaattt
    35161 ggcttgaaac atactggaat gatcgatcaa gggattcatg tcaatatgac agatgaagca
    35221 agtatcgatc atgacggtag tgtacttgat tattcccgat tgcatgatat gacgatccaa
    35281 gcatggtcac cataccagta tggatttttt gaaggtgtat ttatcgggaa cgaaaaattc
    35341 cctgaattaa accaaacatt agaagaacta gcagctaaat atcaaacgac accaactggc
    35401 ttggcttctg cttggatttt acgccatcca gccaatatgc aagtgatcgc cggtacgatg
    35461 aacgtagggc gaatcaagga aatcgctcaa gcttcagaaa ttaatatgtc tcgggaagat
    35521 tggtacgaat tgtaccgtgc tgctggaaac attttacctt aaagattgtc ataagcaggt
    35581 caaaagggct ataaattaaa aagacagaat aacttgaaag ctgttttcag cttcgagata
    35641 ttctgtcttt ttttattgca aatggaaaga gtatcattgt acggatttcg tttattagtt
    35701 atttttgttt ttaaggaaaa gctgtgtccg atattctttt ggagaaatcc cttgtttttt
    35761 cttaaataaa taactgaaat agttagggtc attataacca atttcaaaag caatatctga
    35821 aatacgatag tctgattgct gcaataattt ttttgctaat tcgattcttt gttctgtcag
    35881 ataatcgatg aatgtttggt taaacgattg agaaaaaatc gtactgaaat gagagggact
    35941 taatgcaact tgttgagcga cccaagttaa ggacatatct ggatcggtaa aattttcttt
    36001 gataaaatcc aatgcatggt tcaaaattgg ctgatatttg ttccttgaag gctgtgaggg
    36061 cagagcagtt aagaaagaaa tgaacatata aactgtcgaa cggtaaagcg agtcgtcggc
    36121 gctgattgct aagcgttgac tggtggatgt gcaatgaacc atttcttgcg ggattttctt
    36181 ttctttcagg cagacatgct ggagtagctt agtcaagaca aataaacgat ataatcgtgt
    36241 tcgagttggt gtgccttgtg gtttcgtcaa gtgttcgatg tacttagata cttcgttttt
    36301 cgataatgaa gacaaatcaa gtgagaggtc aaagtaaaag acagaatcaa ctggaagttt
    36361 cgcttcttga atcaaatcct catagctgat aatttgttct ttaagggaac tggcggtttc
    36421 aagtatcatc tgcgtgatct caaaagatgc aggtaattcg ctgattcttt ctgcgacagg
    36481 accaatggcg acagaaaaat tatttttttg tcccttttct aattcatgga ccaacgtttg
    36541 ggctacctga taactggtct cgatcacatt ttcttcaaga gaatcaacta ttaaaaattt
    36601 gatgaatcga gaggaaacag cagagaagat cacttgttga ttctccctaa ataaaaactt
    36661 caaataatca ctgagatgag cgtaatcttc aaagtctgta ctgacttggt ttgctgctaa
    36721 taatacggtc atttttttcc ctgctaaaga gcgattgaaa gattcagcaa gtttcaatgt
    36781 ttcactgatc gtaagtgagc ctttgaacaa accgttcaag aaatgatttt ttttcaattc
    36841 gaaaacaaga tcagaagaag tgtgcgtttc tttttgctgc aaatctaaag cagttactgc
    36901 tttttctatt gcttgtttca attcgctact tttaatcggt ttaagcagat aatcatctgc
    36961 ctgtacctgg attgctcctt tcatataatc gaaatcgtcg aatccactaa taaatatgag
    37021 acgagtccaa ggaagtagtt tccttgcttc ttttgaaaaa gtcaacccgt ccatgaaagg
    37081 catttggata tcagtcagaa tgatgtcggg ctgtaagtcg ataattgaag acagtgccat
    37141 ctcaccatct ccagcttcgc cgacataagt gattggataa taagaaccaa ggacagttat
    37201 ttgttcccgc aagttattgc gaatgaggta ttcgtcttcg atgataaaaa ctttatacat
    37261 gtttgtttcc tccaattgtt ttgggagata gagggatcat cagtgtgaca gttgttcctt
    37321 ctcggtattt gctgtcaatc gtgataggtt tttgatcatt tccgtaataa agaaggagac
    37381 gcttgtttac gttgtaaagt ccgtagccga tactgaaatc tgaatcgatc cctttggcta
    37441 actctttacg tatttctgcc agtttttctg cagggatgcc aatcccgttg tctataactt
    37501 tgaattggat catctcatca attttgcatg cggtaatacg aataaatcct ttgtgacgga
    37561 cgtacttcgt tccatggtat acagcatttt caatcaatgg ctgcaagatc atttttaaaa
    37621 tagaaacagg aaaaagtgtt tctggtatat cgatcttata gtcaagcata tggccgtatc
    37681 ttatttttaa gatgactaga taatcattga tatggctaat ttctttttca agaggaatcc
    37741 agtcttgtcc tttgcttaaa gacgtacgaa aaaagccgga aagtgcatgg gtcatagtca
    37801 gagcttcttc aaagcagtct tgttcgatca aagaaataat ggcatccaaa ctgttataaa
    37861 taaaatgggg agtaatctga gcttgcaata cacgaacttc actttgtgct aaatggtatt
    37921 gtttcaaagc gttctcccgt aaagactggt ttagatcttc cgccatttta ttcatgctag
    37981 cggtaagtgt ttgtaattcc agtgtttccg gcagggttaa acgaaagcca aggttccctg
    38041 aagaaagttg ttctgacatc tcgatcagct gattgatcgg ttcttggacc tttctctcta
    38101 aaaagcgacg attctttttc gcatagaaaa aaatggtact aagaactcca agttccacta
    38161 ttgttaatag aatgagcgag cggacaagtg tcgtattttt tttgcttgct aagtcgatct
    38221 ccacacgaac aaaatcttgt aagatatctg acagcagttg agtgacagaa tctacctgat
    38281 tgatgatctc ttcattggta tccataaaat cttttgccaa gatattattg atcagctcgt
    38341 tttgataatc ttccagtgta tcgatcgtac gtagcgatac atctaacgta ctaatttctt
    38401 taagtgtatt tgtattattt ttgatctctc gaatatcatt cctaagttct tctaggatat
    38461 tttcctcatt gaactcatct acggttactt ggccagaaat caagtcccac atactttcca
    38521 aaactttttg atcaaccttc aaagcaattg catctgcttt ttggatattt gcgatcgtct
    38581 gttgataaat caaaagctgt tgcgaatata acagtatgct cagtagcaaa gggataacag
    38641 caaatagaag catggctttg tttgcttggg caaaaatctg ttttatgctt aaattttttg
    38701 tcatgatact tctccagtca ataatttctg gttgggatgt tagctatatt ctgctgggaa
    38761 aagacagtct cggaaataaa aatatcttta ggtaatgttt ttcctgtttg aatatttcca
    38821 aaataacgag taacggtatt ggctacatac cagccgacat caggtttaca ctcgactaca
    38881 caattgacga ttcctgagcg tagcttctcg atcatatctt cttgtccatc aatagttaca
    38941 atgatcaaat cttctccagg tttgattgat gtttctttta tcgcagctaa agcaccgtga
    39001 gtcatttcat cactatggct gtataataca tcgatttcgg ataaatcatt tgtttctaaa
    39061 tatcgtttca tctggtcttt tcctttcatc cgaataaaat caccgacaat tcggtgaaca
    39121 atagtcatcg gaggtttatg gtcgatggtt ccatcttttt ttagggttgg tttacgattg
    39181 attgattcca aaaatccttc ggagcgtagc ttggtcggtg tggagttctc tgaaccagca
    39241 agttctagga ttctgatttc ctgtttcttt gaatttgtga agtgattgct gacaaataaa
    39301 cccgcccgat ttccttctgc tttgaaactg gggccaatgt gggtgacgaa aagctcggga
    39361 tcattcgttt gaatatgtct gtcgacaagt atgacaggga tccctgctcg ctttgcttct
    39421 tttaatacag agtcccaccc ggattcttgc aaaggagtaa aaatgatcat atccacttta
    39481 taggcaatga acgtccgtat atcttggatt tgccgttcct gattcatgta gccatttcgg
    39541 tagagaactt ggtaatcttc tttttcaagc gcagtgataa ttgattcagt atgctttttc
    39601 cgccagctac tctccgttcc tgattgcgaa aaaccaacga ctagtgggtc aggtgcttcc
    39661 tttgtattag gtgtacaccc catcatttgg taagcgatta caacaagggc taaaaaaaat
    39721 aaaacacgtt tcactttaaa tcactccatc tggatttgtt agtactatct tatcagataa
    39781 aagggaaaat caaagtcaaa aggaatcaat cgaaaaaaat ccaagaaact taaaaaaatc
    39841 caagacaaaa aaagtacacc taaaaaattt aaagtaaatc ccataaaaaa atcaatgtta
    39901 acgctttctt tattagctat actcggtgta tcaattgaaa tgggggataa aaaatgaaaa
    39961 acaaatttag aaagattgtt gggactatcg ctattttgac cgcaagcatt ctattaagtg
    40021 cgtgtggcaa ttcaggatct gcagatgata gtacggggta tgtaggaatc gctatgccga
    40081 ccaaatcagc agaaagatgg atcgctgatg ggaacaacat ggtctcggaa ttagaaaaac
    40141 tgggttataa aacagatctt caatatggag aagataaagt agaaaatcaa gtagcacaaa
    40201 tagaaaatat gattacaaaa ggggtagata cacttgtgat tgcttctatc gatggttcgg
    40261 ctttaactga tgtgttagct aaagcaaaag aagcagatat caaggtgatc gcctatgatc
    40321 gtttactgat gaattcagaa aatgtcgatt attacgcgac ttttgataat tttggagtag
    40381 gtgtatcgca agcagcttat attgaagagc acttaggatt gaaagaagga aaaggaccat
    40441 tcacgattga attatttggt ggctcgcctg acgacaacaa cgcactaatc aattacaacg
    40501 gagtgatgtc tgttttacaa ccatatatgg acaacggtca gttggtcgtg ccatctggac
    40561 agacgagttt cagtcaaatc gctacgcttc gctgggatgg ttctacggcg caagcaagga
    40621 tggataatct gttaagcgca aattatactg accaaacatt ggatgctgtt ttgtcgccat
    40681 atgatccaat aagtttagga atcatttcgt cattaaaagg cgtggggtat gggagtgaaa
    40741 gtaaaccttt acctgtgatt actggacaag atgcgacagt tgctggcgtt aaatcgatca
    40801 ttgccgggga acaaacacaa acgatcttta aagacacacg tatcttagca aaaaatacga
    40861 ttgaaatgat caaagcaatc agtgatggag aagaggttcc ggtcaatgat actgaaactt
    40921 atgataatgg ggtgaaaact gtccctacgt atttagcaaa tacagtatct gtcgataaag
    40981 ataattatca agcagagctg atcgacaccg attattataa agaatccgat ctgaaaaatt
    41041 aagaggaacc aaacaaggaa ctaaagagat ctaagtcaga gagaggataa gaattatggc
    41101 ggattgtatt ttaaaaatgg cagatatcac caaaaaattt tctggaatcc gagcgttaga
    41161 ttgcgtgaat ctgatgattg aacgtgggga aatccatgca ttgtgcggag aaaacggagc
    41221 gggaaaatca acattgatga atgtcttgag cggcttgtat ccatatggct cctatgacgg
    41281 ggaaatcttt tatgatggaa agctgtgtca atttaaagat ttgaaagata gtgaagaaaa
    41341 aggcatcgtc atcattcatc aagaattagc actaagtcct tatctttcaa taaaagagaa
    41401 tatttttctc ggaaatgaac aagcagaaag aggcgtgatt gattgggaac tgacagaaaa
    41461 gaaaacatat gaactgctga ataaagtcgg tttgtctatc gacccgaatc ttctagtatc
    41521 ccagattggc gtagggcaac agcaactagt tgaaatagct aaagcattat caaaatccgt
    41581 ccgtctgttg attttggatg agcctaccgc tgcattgaat gaagaagaaa gcgcgaattt
    41641 gctgagattg atccgagagt tgaaagagca aggtgtaacc tccatcatta tttcacataa
    41701 gctgaatgaa atcgtggata tcgcagatcg aatcaccatt ttacgggatg gacagacgat
    41761 tgagacacta gaaaatgaaa tgatcagcga agagcatatt attcgcggga tggtgggcag
    41821 agatttagtt gatcgttatc ctgaaagaaa accggatatt ggagaagttt attttaaagt
    41881 agaagactgg acggtctatc accctcttga tcacgagcga atcataaacg accacatcaa
    41941 cttgcatttg aaaaaaggag agattgtagg aatagctgga ctgatgggag cgggcagaac
    42001 tgaattcgct atgagtctat ttggacattc ttatggaagc aagatatccg gacgtatatt
    42061 aaaggaaggg gcagagatat cagttaagac cgttccttca gccattgcaa acggactcgc
    42121 ttatgtctcg gaggaccgaa aatctttagg actgaatctg ctgatggata ttcgggaaaa
    42181 cgtgacgctg agcagtttgg ataaaataag tcaaaaagga gtattagata aagaaaaaga
    42241 agtaactgag gcagaaaatt tccggaaaaa gatgcgtata aaaacaaact ccatccatca
    42301 aattgtcagt agtcttagtg gtgggaatca acaaaaagtc gttttatcca agtggctgat
    42361 gacacaaccc gatattcttt ttttggatga gccaacccgt gggatagatg ttggggcaaa
    42421 atatgagatc tacacgatta tcgaagaaat ggcagcaaat ggcaaagctg tctgtcttat
    42481 ctcttctgaa cttcctgaaa tattagggat gtgtgatcga atctatacga tgaacgaagg
    42541 acgtattaca ggagaagtct cgcgagaaga tgcagatcag gaagtcttga tgaaattaat
    42601 gacgaaagaa gaggtgggtt aagttggaac aggcaaaaaa tcttacggat aaaaaagaga
    42661 aaaaactaaa tttgaaaagt agagcagctg atctttttgg tcgctacagc atggtgatca
    42721 ttttagcggg actacttatt gcgtttcaat tgatgacgga tggtattttc tggcgtccat
    42781 tgaatataac taaccttgtt ctgcaaaata gccatatatt agtactagct gcagggatgt
    42841 tattagttgt gctcttaggt catgtggatt tgtcggtagg gtcagtgatg gcgtttgttg
    42901 gtgcaattgc gggcatgatg atggtcaatc gccaaatgaa cccgtggatg acagttatcg
    42961 catgtcttgc agtaggcggg ctgatcggcg catggcaagg tttttgggta gcttatgcag
    43021 ggataccggc gtttatcgtg actttagcag gattgctgat gtttcgtgga ctgacacaag
    43081 ttgttctagg aggccaatct ctagcacctt ttccagctgt tttccaaaaa atgtctacgg
    43141 gttatttacc agacttaagc gaaggaagca gccttcactt cttgacattg attttaggaa
    43201 tgatcatcac gatcgtgtta gttgttggtc aatggcgaac aagaaatcgt cggaaaaatc
    43261 atttatttga agtggaatct ccaacagctt tcttggtaaa ggcggtattt acagcagtcg
    43321 ttgtactagg aataacttat atctttgcct cttatcaagg gtatccgatt attttgatca
    43381 ttcttggttt gatcgttgca gcatatgggt ttttgacaaa taaaacagta gcaggtcgac
    43441 agatttatgc gacgggcgga aatcgaaaag cggctgaact atcagggatc aaaacaaagc
    43501 gaatcacgtt ctgggtattc gtcaacatgg gagtcatggc agctttagct ggattagttt
    43561 tagctgctcg attgaatgcg gcaacacctc aagcaggtac atcgatggaa ctggatgcaa
    43621 tggcggcagt atattttggt ggtgcttcga cttccggcgg cattggaaca atcatgggtg
    43681 cgattgtcgg tggcttggtc atgggtgtat tgaacaatgg catgtcgatt ttaggcgtcg
    43741 gggtagactg gcagcaggca atcaaaggat tgatcttgct tttagccgtt gtgctagact
    43801 tgtataataa gaaaaaacga acaacgtagg agaacgccgg ttattcagaa aaaagactgt
    43861 gacaagacat ttgtcacagt ctttttttcg ctaaaagcga acattcattg tttcgtttgt
    43921 atagactaaa aataatatat gtttttttac cattcgtata aattctcttt ttgctcttga
    43981 tttttttgct taattgagtt aaattacata gggcgctaaa aaaagccttg attaaattaa
    44041 taatgttcgt gttttgctgg atgtgaacat caggaggata atgtaatgaa agtatttgat
    44101 tacgaagata ttcaattaat tcctaataaa tgtatcgtaa atagccgttc tgaatgtgat
    44161 acaacggtca cattaggtaa acatacattc aaaatgccag tagtgcctgc caacatgcag
    44221 acgattattg atgaaacaat cgcagaattc ttagcggaaa atggttattt ctatatcatg
    44281 catcggtttg atgaagcagc tcgaattccg tttatcaaaa aaatgaaaaa gcgtggattg
    44341 attacctcga tcagtgttgg cgtcaagaaa gaggaatatt cctttataga gaagttggca
    44401 gaagaatcgc taaatcctga ttatatcact atcgacatcg cgcatggaca tgctaactca
    44461 gttattgata tgattcagca tattaaaaaa tatttgcctg aaacttttgt gattgccggt
    44521 aatgttggta caccggaagc cgtccgtgaa ttggaaaatg cgggcgcaga tgcaacaaaa
    44581 gtcgggattg gtccaggaaa agtatgtatt acaaaaatca agacaggctt tgggactgga
    44641 ggctggcaac tggcagctct tagatggtgt gcgaaagcag ctcgcaagcc gatcatcgct
    44701 gacggtggga tcagaacaca tggcgatatt gcaaaatctg tccgttttgg tgcgacaatg
    44761 gtcatgattg gttcattgtt tgccggtcac gaggaatctc ctggcgaaac aaaagtcgaa
    44821 aatggaatcg tgtataaaga atactttggt tctgcttcag aattccaaaa aggcgaaaaa
    44881 cgaaatgttg aaggaaagaa aatctggatc caacacaaag gatctttgaa agatacacta
    44941 gtggaaatgc aacaagacct tcaatcatct atttcttatg ctggaggaag agatctggaa
    45001 gccattcgaa aagttgacta tgtcatcgtg aaaaattcga tttttaatgg agacacaatc
    45061 taaaaaataa ataaaatatt aggatgttgt gaaaaagttg tcctgcatta agtaataaga
    45121 acagccaaac aaaaatagtt ctttatatgt tttgtttggc tggaattatt atcgcagata
    45181 caagcttttg aacatcatta ttcagaataa gggactgtga taagattttt gtcacagtcc
    45241 cttatgtttt gcaaaaaaag tttttctttt ttctcgtaaa ttcggtaaaa tcaaaagaaa
    45301 aaatagacaa tagaaaagag aagcttatgt gggaaaaagt agagaaaaaa caaggaccga
    45361 tttatcgtca aattatggaa cagattatga gagaaatcga aaacggaagg atcaatcctg
    45421 gaagtcaact tgaatcagaa cgaaaactag ggcaactttt tggtgtgaat cgttccaccg
    45481 ttgtccatgc attggaagag cttagggagc ttggggtact aacgagcaaa cgtggcagcg
    45541 gatggtttgt taatcagact gaatggggga aattcgctgt tccaagaatc gattggcggc
    45601 aaatgatctc tcctcgttat gagcagacgg actggtatga gcaaaaaata agagaagcaa
    45661 agcagatcca aaaaacatcg tttcttgatt tatacgctag cgagatgcca gatgagcttt
    45721 tgccgaattt tgagtttcct tcttattctc ttgaagaaat cttagcggaa gaaaaagaaa
    45781 tggatctttt aggctatact ccgttacgta acaaaatcaa acggtatttg gaaagtaaat
    45841 ttgaattggc attttccccc aaccagcttc ttgtgactgg tggcggtcag caagcaatct
    45901 ttttgatttt acaaacgatt ctttcagctg gagaggggat agctgtagag tcaccttctt
    45961 ttttttaccg attacctctt ttcaaagctt caggaacgcg tttatttggg ataccgatgg
    46021 atgaagaagg gatcgactta gaaaaactga aagaatcgat ccataaaaat aaattgaaag
    46081 cagtgcttat caacccgaat tttcaaaacc ctacaggaaa agtgatgagt caaaaacgac
    46141 gagaaaagct agtagaattg tgtcgacagt atcggcttcc aattattgaa gacgatgtat
    46201 ttacggatct ttcatttcta aaccaagaag aagtccttcc tatacgaacg attgatccgg
    46261 ataatgtgtt atacgtcggt tcgttatcta gggttttagg aaaaacaacg aaaatcggtt
    46321 gggtgatagg acctgagact tttatctcgc aactagctca agcgcaagaa atgatggagt
    46381 tttcgatgaa tatccttaca caaatcacag tggctaatgt atttaatgaa gcaattgatt
    46441 tgaaaatgca agatgtaaga aaaaagttga aagaaaaaag ccgactgata agcgcttggg
    46501 gagattcaca atcgtttttc aagcttcatt caattttagg tggttactat gcctggatta
    46561 cttgggatgg gaaaaaacta gaccccgaac tagcagaaaa agtagtagat atgggtcttg
    46621 gaattgcacc aagtctactt tttgggcaag atataaatgg aatcagaatc aattacagta
    46681 ggctggatga aaaccaaata ccgttgtttt attcaaagat gaaaattcta gaaaagtggt
    46741 taacagaata atttcacaaa gtaaatagat taaagaatat aacagcttgg atttaaattt
    46801 caagctgttt cttttttttc ggaaaaagaa tacgatataa agaaactaag aaaaaaccat
    46861 tatttgtacg tccgttcacg atgtgtaaat aaaataaatg tttgcaacaa aactgtataa
    46921 caccaacgtt tttaaataga aacagatggt tgtaagtttg attagaaaat agaaaaaatg
    46981 cagatgaaaa ataaaacgct ttccttattg tgattttatt cactaatgct gttaatatgc
    47041 ggattggcat aatttccttt catcgtgaaa atttgtgctt ttagcttgta tttctcataa
    47101 atccatgata atttattaat gaagacggtt aaatagtgat acagttttat tcactgtttc
    47161 aatcgcagaa tgaagagatt ttcttataga tgagagagga atgtgtgaca aatggcaaac
    47221 aaaaaagcac cgatcgactt ccaagcactg ctagatgaag tcaatgcaga tttcccagta
    47281 tatcaagcac ttgatcaaga tggaaacgta gttaacaaag atttagtacc tgatttatcc
    47341 gatgatgaac tcgttgaatt aatgactcat atggtatggt ctcgtgtatt agaccaacgt
    47401 tcaacagcgc tgaaccgcca aggacgccta ggcttttttg caccaactgc tggacaagag
    47461 gcaagtcagc tggctagtgc ttacgcattt gataaagaag atgttcttct tccaggttat
    47521 cgggatgtac ctcaactagt aaaacatggt ttacctttat ctcaagcttt tttatggtct
    47581 cgaggacatg ctgcaggtaa ttcttatccg gaagaattga aagctctacc accacaaatt
    47641 atcatcggtg cacaatatgt acaagcagcc ggtgttgcat taggtttgaa gaaacgaaac
    47701 aagaaaaatg tcgtcttcac ttatactggt gatggcggtt catcacaagg tgatttttac
    47761 gaagcaatca atttcgctgg cgcttaccat gcaaatgccg tattttatat ccaaaacaac
    47821 gggtatgcga tctctacacc tcgtgaaaaa caaactgcag caaaaacact tgcacaaaaa
    47881 gctgtcgcag ctgggattcc tggtattcaa gtcgatggaa tggatccgct tgcagtatat
    47941 actgtatcta aaatggcacg ggaatgggcg atcagtggaa atggaccagt cctaatcgaa
    48001 acactgactt atcgttatgg accacataca ctatctggtg acgatccaac ccgctaccgt
    48061 acaaaagaca cagatgacga ttggcagaaa aaagatccat tgatccgctt ccgtaaatat
    48121 ttgacggaaa aaggactttg gtctgaagaa aaagaagaac aagtcatcga agcaactaaa
    48181 gaagaaatca aagcagctat tgctgaagcc gacaaagtac caaaacaaaa ggtttcagat
    48241 ttcttgaaaa acatgttcga agtatctcca caatcaatca aagaacaaat tgcaatttat
    48301 gaagcgaagg agtcgaaata atcatggcac aaaaaacaat gatccaagca atcacagatg
    48361 ccttagcact tgaactagaa aatgacgaga atgtcgttgt ttttggtgaa gatgttggga
    48421 aaaacggagg agttttccgg gcaactgaag gattgcagga aaagttcgga gaagaccgag
    48481 tattcgatac tcctttagct gaatctggaa tcgcaggatt gagtttcggt cttgcgttag
    48541 aaggatttcg tccagttcct gaaatccaat tctttggttt tattttcgaa gcaatggacg
    48601 aagtagtagc tcaaatggct cgtacaagat atcgaatgag cggcactaga aatctaccca
    48661 tcacaattcg ttcgccattt ggtggcggtg ttcatacacc agaattgcat tcggataatt
    48721 tagaaggttt gatcgctcag tctcctggta ttcgagtagt gattccttcc aacccttatg
    48781 atgcaaaagg actattgatt tcttctatta gaagtaacga tccggttgtt ttcctagaac
    48841 acatgaaact atatcgctca ttccgagaag aagtgccgga tgaagcttac gaagttcctt
    48901 tggataaagc agcagttact cgtgagggaa cagatgtatc tattatcaca tacggagcaa
    48961 tggtgcgtga agcaatcaaa gcagcagaca atcttgcaaa agaaaatata tctgttgaaa
    49021 tcattgatct tcgtacagtt gcgccattag atgtagaaac gatcattcaa tccgtggaaa
    49081 aaactggtcg cgtagttgtt gtccaagaag cacaacgaca agcgggcgtt gccgcgcaag
    49141 tagtttctga gatctcagaa cgcgctatcc tttcattaga agccccaatc ggacgagttt
    49201 ctgcacctga tacagtcttc ccatttggac aagcagaaaa tgtctggttg ccaaatgcaa
    49261 aagatatcga agacaaagta aaagaaatcg cggaatttta agaaaagaag ggacgaaaca
    49321 aagtcatggc ttatcaattt aaatt
//
LOCUS       DAZUKR010000005        46245 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000005 DAZUKR010000000
VERSION     DAZUKR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.005"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..1124)
                     /locus_tag="ACG89C_000302"
     CDS             complement(105..1124)
                     /locus_tag="ACG89C_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4898888.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1146..2153)
                     /locus_tag="ACG89C_000303"
     CDS             complement(1146..2153)
                     /locus_tag="ACG89C_000303"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4898889.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="ACG89C_000304"
     CDS             complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="ACG89C_000304"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4898890.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(2804..5098)
                     /locus_tag="ACG89C_000305"
     CDS             complement(2804..5098)
                     /locus_tag="ACG89C_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS4898891.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5358..7523
                     /locus_tag="ACG89C_000306"
     CDS             5358..7523
                     /locus_tag="ACG89C_000306"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS4898892.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7827..8660
                     /locus_tag="ACG89C_000307"
     CDS             7827..8660
                     /locus_tag="ACG89C_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4898893.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8762..10462)
                     /locus_tag="ACG89C_000308"
     CDS             complement(8762..10462)
                     /locus_tag="ACG89C_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898894.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10969..12129
                     /locus_tag="ACG89C_000309"
     CDS             10969..12129
                     /locus_tag="ACG89C_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4898895.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12145..12588
                     /locus_tag="ACG89C_000310"
     CDS             12145..12588
                     /locus_tag="ACG89C_000310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4898896.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13071..13601)
                     /locus_tag="ACG89C_000311"
     CDS             complement(13071..13601)
                     /locus_tag="ACG89C_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS4898897.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13706..14257
                     /locus_tag="ACG89C_000312"
     CDS             13706..14257
                     /locus_tag="ACG89C_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4898898.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14410..14883)
                     /locus_tag="ACG89C_000313"
     CDS             complement(14410..14883)
                     /locus_tag="ACG89C_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4898899.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15021..16493
                     /locus_tag="ACG89C_000314"
     CDS             15021..16493
                     /locus_tag="ACG89C_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4898900.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16490..17200
                     /locus_tag="ACG89C_000315"
     CDS             16490..17200
                     /locus_tag="ACG89C_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS4898901.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17552..17836
                     /locus_tag="ACG89C_000316"
     CDS             17552..17836
                     /locus_tag="ACG89C_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS4898902.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18117..18536)
                     /locus_tag="ACG89C_000317"
     CDS             complement(18117..18536)
                     /locus_tag="ACG89C_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS4898903.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(18538..19122)
                     /locus_tag="ACG89C_000318"
     CDS             complement(18538..19122)
                     /locus_tag="ACG89C_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS4898904.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(19169..20644)
                     /locus_tag="ACG89C_000319"
     CDS             complement(19169..20644)
                     /locus_tag="ACG89C_000319"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS4898905.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20825..21136
                     /locus_tag="ACG89C_000320"
     CDS             20825..21136
                     /locus_tag="ACG89C_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898906.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21251..23587)
                     /locus_tag="ACG89C_000321"
     CDS             complement(21251..23587)
                     /locus_tag="ACG89C_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4898907.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23729..24397
                     /locus_tag="ACG89C_000322"
     CDS             23729..24397
                     /locus_tag="ACG89C_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4898908.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24400..25443
                     /locus_tag="ACG89C_000323"
     CDS             24400..25443
                     /locus_tag="ACG89C_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4898909.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25680..26561)
                     /locus_tag="ACG89C_000324"
     CDS             complement(25680..26561)
                     /locus_tag="ACG89C_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4898910.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26720..27448
                     /locus_tag="ACG89C_000325"
     CDS             26720..27448
                     /locus_tag="ACG89C_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4898911.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27604..27921
                     /locus_tag="ACG89C_000326"
     CDS             27604..27921
                     /locus_tag="ACG89C_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4898912.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27914..28522
                     /locus_tag="ACG89C_000327"
     CDS             27914..28522
                     /locus_tag="ACG89C_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS4898913.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28577..29368
                     /locus_tag="ACG89C_000328"
     CDS             28577..29368
                     /locus_tag="ACG89C_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS4898914.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29506..30018
                     /locus_tag="ACG89C_000329"
     CDS             29506..30018
                     /locus_tag="ACG89C_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4898915.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            30044..30916
                     /locus_tag="ACG89C_000330"
     CDS             30044..30916
                     /locus_tag="ACG89C_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4898916.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31092..31454
                     /locus_tag="ACG89C_000331"
     CDS             31092..31454
                     /locus_tag="ACG89C_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4898917.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31463..32215
                     /locus_tag="ACG89C_000332"
     CDS             31463..32215
                     /locus_tag="ACG89C_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898918.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32229..34031
                     /locus_tag="ACG89C_000333"
     CDS             32229..34031
                     /locus_tag="ACG89C_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898919.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34308..35348
                     /locus_tag="ACG89C_000334"
     CDS             34308..35348
                     /locus_tag="ACG89C_000334"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4898920.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(35400..37544)
                     /locus_tag="ACG89C_000335"
     CDS             complement(35400..37544)
                     /locus_tag="ACG89C_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4898921.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(37901..39274)
                     /locus_tag="ACG89C_000336"
     CDS             complement(37901..39274)
                     /locus_tag="ACG89C_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4898922.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(39274..39669)
                     /gene="rbsD"
                     /locus_tag="ACG89C_000337"
     CDS             complement(39274..39669)
                     /gene="rbsD"
                     /locus_tag="ACG89C_000337"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4898923.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(39697..40614)
                     /gene="rbsK"
                     /locus_tag="ACG89C_000338"
     CDS             complement(39697..40614)
                     /gene="rbsK"
                     /locus_tag="ACG89C_000338"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4898924.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(40722..41735)
                     /locus_tag="ACG89C_000339"
     CDS             complement(40722..41735)
                     /locus_tag="ACG89C_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4898925.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(41816..42415)
                     /locus_tag="ACG89C_000340"
     CDS             complement(41816..42415)
                     /locus_tag="ACG89C_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4898926.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            42601..43302
                     /locus_tag="ACG89C_000341"
     CDS             42601..43302
                     /locus_tag="ACG89C_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898927.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            43312..46023
                     /locus_tag="ACG89C_000342"
     CDS             43312..46023
                     /locus_tag="ACG89C_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4898928.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
BASE COUNT        14316 a         8331 c         9460 g        14138 t
ORIGIN      
        1 cacgctcttt tttacagata aacaatgtgt tagaagtagt atcttctttt acataaaaag
       61 ctgaccatag gataaatggt cagcgtattt agtgagtcag ctatttagta tgggttttct
      121 aaaggaacga ctgtctctct tttgatcaag cgatgaggga tgaccaaacg tacgccactg
      181 ctcaattctt catggatcaa ttcgcgcagc ttttgcatag cgacacgtcc taattcggag
      241 atatcgatat cgatacttgt aaggtaaggg tgtgttaatg ttgagaagat cgagttgttg
      301 aagctaatga cggagagtgt ctctggaatg acatagccgt acatttgcgc caactgcatc
      361 gtgcgaaggg cgaagatatc gtcaatgacc accaatgctg tggcttcgtt ttctttcaac
      421 gttttctcaa aggacagata atcctccgga tgctctagta ctacactgga taacgcagga
      481 agaccagcca tcatcattgc tttttgataa ccgaaatatc gttcgaaata caaactttca
      541 tgagtcgtgt ttgtcacaaa caggatattc ttatgtccat tctggatcaa aaattctgtt
      601 gcttgtttac ctagaagctg gttatcatta tctacataaa cgatttcgtt ctcatgatga
      661 taaggctgtc cgatcaaagt gaaagggatg tggttttggt ataaataatc aatgacagga
      721 tcttgactat ctgaatacac tagaataaaa ccatctactt gtttttgtaa atgcatacgc
      781 tggacgttct ctaacagcaa ttcaaaattt ttagcagtag cgattgctgt agtcatatca
      841 taattccgcg cttcttcgtt gattgcttcc atgatctcaa gataaaatgg gttaccgatc
      901 cgctcttttg aatcaagagg gggtaagatt acgccgatgg cactggaaat gcgttttcct
      961 agattgcggg cagtcatgtt aggaacataa cctagttcat ccataatttt tcggactttc
     1021 ttctttgttg tctcggaaat actaggatga tcgttgatga cacgagagac ggtcgaggtg
     1081 gcaacaccgg cttttttcgc cacatctttt acggtaatag ccatgataaa aacacctttc
     1141 tcttgttatt gtgcagagaa ccgataaatc gtctgactga tccgttcttg accaggatgg
     1201 aggttgatcg atccaaattt ggggaaatga acgatatcag ggatttcttg cggttcgatt
     1261 gctaatccgt agtttgaatg catctgtttt ccattgacag agaaatcagc ttcgaatcca
     1321 gtcgtagaaa ataataccat acttttatta gaagttgcta tagtcatttg gcgaccagaa
     1381 tgcttctcga ataattgtaa ggaagttttg gaaagcctag gatgatgcaa aagaaataca
     1441 tcatctagtc catctggata acatgcaagg atctcttgca gtgttgggga ttttctaaag
     1501 tcgattggca gtttttcttt ttttgtccaa tttccagtag gcagtttttc ttgatctaat
     1561 tcaagcacac catctaaaaa gaccgttaac tgatgttcat agatgtctct tgttccattt
     1621 cctgaaaggt tgaagtaggc atggttggtc ggattgcaga tggtttcaac aaggctacta
     1681 gcagaggttg tcatctctaa ttcgtttttt gttaaacggt aagtgatcgt agcagtgatt
     1741 ggtcctggat aggtagaaaa ttcatctttt aagtaaaaga ccacacctat tgatgatgga
     1801 gttttgaaaa cttctacttc ccaaaattgg aaagaccagc ctttcgtgcc accgtggata
     1861 tggtgtgatc cgcagttttt ctccaatcta tgctcacccc aagaagcatc ccggatcctt
     1921 ccggctacag gtccgacagt tgctccaaaa aagcttttgt ctgttagaac atcttcgaaa
     1981 cgatcgtaag acaataaaac attttctgat tttccttctt tgtctggtgc gaagatttgg
     2041 tgcaggcggg caccgtaatt gagaagaacg acttctaagt agtcgttctt caatgtgatt
     2101 ttctttattt cagtctctgg aactggtacg acggagatct ctggattatc catggtcatg
     2161 acacttttct ttcaagtaat cgatcgtaag aaaagtcgta tcagggacaa gcgggatctc
     2221 ctctccaagg tcttcagcac gtcccacgcc aattggttga gcgccacttg agaggatggc
     2281 ttggataccg gcttgcgcgt cttcgatccc gatacattct tctgctttta gtccgacagc
     2341 ttttgctgca aggaggaaga tatctggcgc aggttttccg tttgttgctt ctgccgggtc
     2401 ggcaatagca tcaaagtaag gtgtaagacc cattttttca agtaggaatg gcccgttttt
     2461 acttgcagaa gcaagagcaa tcttgatttt ttcttctttt aaagaatcca gtaaaggcac
     2521 gaccccgggg aagacatctt ttggttcgat cgtctggatc atctctaaat aataatcgtt
     2581 ttttcttttc gctaattctg caaattcttc ctttgaaaaa gagtgttctt ttccgccata
     2641 agctaaaagc aaagagagtg agtcttctcg gctgacgcct tttagctgtt cgttgaatgt
     2701 gtggtcgatc gtaatgccga tttcctgacc aagctttcgc caagcacgat aatggaattc
     2761 agcagtatcg gtaatgacac cgtctaaatc aaacaatacc cctttaaaca tttgcttcct
     2821 cctttcttag cgttatttct agacgatccg ctaaagaata agactgttca tagacgtaaa
     2881 tttctagagg aaaaccttct aataactcga aagtaacttg ttcatctgtg actgagatga
     2941 acaataaacg tccgcgataa ttgatatgga aagaataagc tttccaagtt tttggcagaa
     3001 atggtgcgaa tctcaactga tcatgggcag ttttcatttg agcaaatcct tggacaatcg
     3061 acaaccagct accagtcatg gaagtgatgt gcaagccgtc ttctgtgtcg ttattgtaat
     3121 tatccaaatc caagcgagct gttcgctggt acatttctac agctttgctt tccattccta
     3181 attctgctgc taaaatcgaa tggatacttg gcgaaaggga agactcatgg acagtcattg
     3241 gttcatagaa atcaaagttt ttttgctttt cttcttttgt aaaagtatca ttgaagaaat
     3301 aaatcccctg tagtacatct gcctgcttga tgaagcaaga tctaaggatt ttgtcccaag
     3361 accaatgctg attcagagga agatcgcttg tgggaatatc ggatacaggc atgagatctt
     3421 tatctaagaa tgtgtcatgc tggacaaaga ttcctaattc ttcgtcaact gggaagtaca
     3481 tgttctctgc gatatctttc catttggcta gttcttcttc gctgatagtg atagcagctt
     3541 ctttttgata agttaaataa ttttctcgcg tgtaacgcaa gacccaagct gcgatcgtat
     3601 ttgtatacca attgttattg atattgttct catattcgtt tgggccggtc acaccgtgga
     3661 tcatgtattt atttgctcgt tttgaaaaat ggacacggtc tgcccagaag cgtgcgattt
     3721 ctgctaaaac ttccagtcct tcatttttca aatattccgt atcgccggtg taattcgtat
     3781 aattgtagat tgcataggca atcgcgccat ttcggtgaat ctcctcgaat gtgatttccc
     3841 actcgttatg gcattcgaca ccagtgaagg tgaccatagg ataaagcgcg ccttttaatc
     3901 cttgttgctg agcgttgtgg attgcttgcg gcagttgatt atgccgatat ttcagcagat
     3961 tcttagttac ttcaggtttg gctaaagcaa gataaagagg gacagcatac gcttctgtat
     4021 cccagtaagt ggctccgccg tatttttctc ctgtgaatcc tttggggccg atattcaatc
     4081 gttcatcttc gccgtagtaa gtagagaata gttggaacaa attgaatcga ataccttgtt
     4141 gtgcttcttc gtctccttcg atgaccacat cggcaagctt ccatcgttct gcccaagccg
     4201 ctgtttgctc ttcttttgct tggatataat tgtttgtctg gttcgttaat tctgagacag
     4261 ctttttctgc ttgctgcgct tctggaatat ctctactagt aagaacgata atttcttttt
     4321 ccactgacca tgtctcacct tgagataatt ctgcagacaa gcaaccatgt aatgctaaag
     4381 tatcttcgtg atagtcaggg atgactgatt ttccattaac gaaatggcgc attgctgcag
     4441 taacagtaaa ttgttcaatc ccaaagttgt ttggaatagt tttagtcgtc aaatagccga
     4501 tttcttctat agttccacga tcaatctcat tccaaaaatg ttcatcatag ttgctgtctt
     4561 cgttttgaac atttccatcc aattttgata cgagtttgac tgttccgcta ccttcaagca
     4621 tttctatagt taaatagata taagctgatt cttttttgac aatgctcaga aaacgttcga
     4681 aggaaatgcg tactttatta gtagaagtag tgattgtgaa tgtacgagat aatacaccat
     4741 ttttcatatc taattcccag taaaagtctt ctggttccaa agaagccaag tcgatcagct
     4801 gatcgttgat cgaaatatct atcgcgataa agttcaaggc gttgatcact tttccaaagt
     4861 attctggata accatttttc caccagccga cacgtgtctt atcgggatac cagacacccg
     4921 ccaaataagt accttgatgg tggtcccctg agtaagtttc ttcaaagttc ccgcgcatcc
     4981 ccatataacc gtttccaata cttgttaaag attcttgcag tcgaagattt tttttatcta
     5041 attgtgtcgt gctgattttc catggatcaa tagcaaatag tcgtttgatc tgtttcattt
     5101 ttctcctcct aatgagctgt tttatacgtt cataataaac gcaaccgatt gcacaagtca
     5161 atagacaaaa gaaaatattt catagaaaaa agcttaaaaa aataaatttt taggagaaaa
     5221 ttatagaagg gaacacacgt attataggtt ttatagaatg gtatagacag aaaaataaaa
     5281 aataaagcat ttacaaaaaa gaaaacgcac gctataatac gtttaacgaa accgattgcg
     5341 taaaggagct aaacggaatg aaaaagatat ttagttttga gttttggcaa aaatttggaa
     5401 aggctttaat ggttgtggta gcagttatgc cggcagctgg gttgatgatc agtataggga
     5461 aatcaattcc cttgatcaat cctgattgga cgccgcttgt cacaacgggc ggagtaattg
     5521 aaaatattgg ttgggcaatt attggtaatc ttcatatttt atttgcttta gcaataggag
     5581 gaagttgggc aaaagaaaga gcaggcggag catttgcagc tgggatctca tttattttaa
     5641 tcaatcggat cactggggct atctttggtg tcacaagtga catgcttgcg aatgaagatg
     5701 catttaccca cactctgttc ggtacgaaga tcatggttaa aggatttttt acaagtgtat
     5761 tggaagcacc agctttgaat atgggagtat tcgtagggat catcgctgga tttgtcgggg
     5821 caatggctta taataagtac tataattata gaaaattgcc ggatgctttg tcatttttca
     5881 atggaaagcg ctttgttccg tttgttgtta tcttatggtc aacaatcgtg gcattgattc
     5941 ttgctgtggt gtggccaaac gtacaggctg gaatcaataa ttttggttta tggattgcag
     6001 aatcacaaga aagcgcaccg attctagcac cgtttttatt tggaacatta gaacgtttac
     6061 tattgccatt tggattgcat catatgctga cgattcctat caactataca cagcttggcg
     6121 gatcgtatga gattttatca ggcgcacagg cggggacaca agtatttggg caagatccgt
     6181 tatggttggc ttgggccact gatttagtga atcttaaagg tgcaggggat atgtcgcaat
     6241 atgagtttgt gttgcataac tggacgccgg ctcgtttcaa agtaggacaa atgattggtt
     6301 cttcaggtat cttgatggga atggcttttg cgatgtatcg taatgtcgat cctgataaga
     6361 aagcaagata taaatcaatg tatttttcag cagcgttggc tgtctttcta acaggtgtaa
     6421 cagaaccatt ggagttcatg tttatgtttg ctgctgtgcc attatatgtc atttattcag
     6481 tgatccaagg ggctgcattt gcgatggcag atatcttgcc attgcgggtc cattcatttg
     6541 ggaatatcga attgctgact cggacaccgt tagctatcaa agcgggtcta ggtggagatc
     6601 ttatcaactt cgtgttgtgt gtgatcgctt ttggtgttgt tacgtatttc ttagcaaatt
     6661 tcttgatcaa aaaattcaac tttgctacac cgggacgtaa cggaaattac gacaatgaca
     6721 gtgaagaaac cgttgtatct aacagcggaa caggaacagc agatcagcaa gtggtccaga
     6781 tcattcattt gctgggagga aaagaaaata tcaaagatgt cgatgcttgt atgactcggt
     6841 tacgcgtaag tgtaaatgat cgagaaaaag tagggactga agaagcctgg aaacgagcag
     6901 gggcgatggg attgatcgtc aaagacaatg gagtccaagc agtttatgga ccaaaagcag
     6961 atgtcttgaa atcagatatc gaagatctgc tgcaatcagg agcagagatt ccagagccag
     7021 aaatggtttc tacaggaaat ggacaagcag gtagtaagca atacttaggt atagaacaag
     7081 aactagtttc cgcagcttct ggagaagtga ttcctttatc tgaagtaaaa gatccggtat
     7141 tctcacaaaa aatgatgggt gacggctttg ctgttattcc aacatcaaga gaagttgttg
     7201 cgccgatcgc tggaaaagtg acaagtattt tccctagcaa acatgcaatt gggctggaaa
     7261 cgaaagatgg tatcgaagtt ttgatccata tgggaatcga tacagtccaa atgaatcagc
     7321 cagcttttga gattgctgtc aaagaaggcc aagaagtagg aacaggaacg aaattagctg
     7381 agatggatct tgatgtgatc caaaacgaag aaaaagatcc aacgatcatg atcgtattta
     7441 caaacgataa agtagaagaa gtagtgatca agcaattagg tacagtgaca gctggtaaag
     7501 tgatcggctc cattaaaatg taaggagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     7561 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgag
     7621 aagcagcagt ttgaatactg tttagtggag aggtcgtgaa aaagctgccc tgcaccaagt
     7681 gataaaaaca gccaaacaaa aaatagctcc tggtccattg agcttattac tgaggagtta
     7741 gcaggagaac actatttgct ggagaggttg tgacaaaaat tttgtcacaa cccttttgtt
     7801 tcgaaatgaa gacaaggaga aaaccaatga aattcatgac actcaacacg catagctggc
     7861 ttgagccaga accagagaaa aaattgcaag aattagcaga taagatcctg ttagaagatt
     7921 atgaagtgat tgctttacaa gaaatcaacc aattactgga atctgaagaa gtagaacctg
     7981 cgaagctaat gaagttctgt caggtaaaga atcaagtacc tatccgtaaa gataattttg
     8041 cctaccgttt agttcagctt ttaaaggaac acggaaaaga gtatttttgg agttgggaaa
     8101 tgagccacat cggttatgat aaatatgaag aaggaaatgc actgcttacg aaaaaaacat
     8161 tggaaagtca ggtccttacc gtctcccaat ctcaaaagaa agaagattat cagacacgca
     8221 agatcttgat tgggaaaaca aagatagatg atcaggacat ttttgtctcg agctgccact
     8281 tttcttggtg gacagataaa aaaagtggct tttattttga gtggaaaaac ttggaaaact
     8341 actttttgga aactcgcgta ccactttttt tcctaggtga tttcaataat ccagtagact
     8401 ctcaaggtta ttatactgta agagaaagtt gccttcttct tcaagattcg tatgttgtcg
     8461 cgaatgaaaa aggaaaagca gctactgtgg aaaagaaaat agatggttgg gaacaaaata
     8521 cagaaaaatt gcgtatagat tttattttcg tccctgaagg aatgcaggtt aaaaaatatc
     8581 aacggatttt tgatgggatc gatagtccaa tcatcagtga tcactacggg gtggaaatcg
     8641 aattagatgt gaacgaataa aaaatataac atataacata aaaaaactga ttaaaataaa
     8701 actctccaaa cgattatacc gtttggagag ttttttgatc ggatgtattt atcccttttg
     8761 cctaagcacg taaaagcttg tatctatgat aaataaataa acatagcaag gtaaaccagc
     8821 caatcaaagt aagagaaaga atgaaataga aatagattgg cgtttgataa tgaagcgtga
     8881 gctcgttgat tccttttttc tgcttcacga ttggggtccc gatatctgtt acttcataat
     8941 cagtcaatgt ttttccattt tgctgtagca cagtccgatc gtagacgata acagggatat
     9001 gaacagtttt gtccccagtg tttttccact tgattgtcaa aagaccgcct ggatgtgttt
     9061 ttataaaagt tgtttcgttt tctagtattt cttcgttata ccggtcgtat ttattgtctt
     9121 ttgtctcctt atagattggc agatagtcag gtgtggtttt ttggaaaacg aacaagctgg
     9181 tagacatatc tttgctgaag aatgttttcc ttgcttcttc cggcgtatcg aatagataag
     9241 tatgtcgact gacaaatgtt tcattttccc acttttccag atgctggtat aagttctgga
     9301 tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa aagacggaac cacttttgtt
     9361 cagaccactg gttcagtgaa agggcaaggt aaagtagaag caatagcgta aatggcacga
     9421 aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac atttttttcg atcaatgtcg
     9481 tccatggaat caagctagta gaaagaaaaa agaaaacaag acttgtgtac aacacgattt
     9541 tttggaataa ggtgttcttt tgttgaaaga aaataaaaag caaaaaacct gataatatca
     9601 atataggaaa ggccttaggg taatggagcc aataactgcc atccagatct atcgcctttt
     9661 gactcatttc tctgttaaca aagggaggaa gaatcttatt cccactgtaa acaatagcta
     9721 atcctgccca aatatttgct gtcagaacaa aataaagcaa gaccgactta atcagttgaa
     9781 gcagtgcttt tcctttttca gtctggttaa aaaagtaata aagataaaaa gggatataca
     9841 tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt cataagtccc actgagacag
     9901 cgactttgat aacagggaac tttttatgaa ggacgaaatc agcaataggg atcatacata
     9961 aaggataaaa acaagtcccc cagctagtga atccttggtt gatagaccag tattgaatag
    10021 aatacgtact catgaataaa acggctactg gtacacttac ccaatttcga acattgacat
    10081 atctcagtaa aatcagcata gaaaatcctg aaataagata aagaatgaaa tttgaaagaa
    10141 tctgataatg aaaccagcta cctgcaagca acacgatcaa tccttgtaaa taagcgaaaa
    10201 atgggccgta aacggcattc acgatcctgc cactttgctg gaatccataa agggaaggaa
    10261 aataatggaa atccccatat ttaatttgtt gcgcggcatc atagaaccga ttgtaatgga
    10321 atggggcatc agcacccaaa attagtttat gtgtgaataa atgaggcatg agaaatgcca
    10381 aagcaatgaa aaaaattgct aaaaatgggc catatttttt caattgtcga actcctttgc
    10441 aaactttaga taaattcatc atatatccta tgcgagaggt aggcaagaaa ttagaaaatt
    10501 catgaaaata tttatgaatt ttacaagaaa aacataagtg aaagttctaa ggagagagga
    10561 tgcgacataa gttttgttgc atctccttgt tttgaaacag aatttttctt aatactcgaa
    10621 acaaagtagc tttttcatga cctctcggta aacggtgttc aatcagctgc ttctcggtaa
    10681 taagctcact agcctagaaa atatgagaag ctgttttctg actagcgctc ttattactca
    10741 aagcataacg cagttttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    10801 gtaataagcc caacccaaca aaaaatatga gtagctattt ttttgggctg tacttattac
    10861 ttgatgcagg acagcttttt cacaacctca tcggctttca ttttctattt ttttcctgaa
    10921 cccatataat aaaagaaaga acaaaaaaac ttgcaaggga ggacacatgt gaagcagagt
    10981 aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt tattaatatg tatcgtcgta
    11041 gggttactga tcgctttata tccattttat gtggattctt taaatagttt tattgatcaa
    11101 aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa ttgaagtgca gcgaaagaaa
    11161 atggaggaga aaaaacaacg attaactgac caaggattca atcctggtac tgatcctttc
    11221 aatgaaaaga acagaaatga aagtacaaca tccagccaat tagaggaatg gctgatcgga
    11281 agtatcaata tacctaagat ccaattaaat atttcacttt atgatcggct caatggtagg
    11341 atccttgaaa atggagcagg cgtcttgcaa ggaacctctt tcccgcttgg tggaaattcg
    11401 acgcattcag tgatctctgc tcacagcggt ttacctaatc gacggttatt taccgaatta
    11461 gatcgtttag aaaacggaga cacgtttatt ttgactgttt tgggggagaa attggcttat
    11521 caagttaaaa atatacaagt ggtactgcca gatgacacgt ctgttctaac gatttcagaa
    11581 ggagaagact tagttacctt gcttacatgt acgccgtata tgatcaacac gcatcgtttg
    11641 ctagtgacag gacatcggat tccttataac gaatcggtaa aaaaagaaga aggaaaagga
    11701 aatcaggaaa gaaccatgcg gcaatggttt attcttgcag gaacgataat agctgtagtc
    11761 atactgttag tgtttatcgg acgactaatc tatcagtata gattaagtaa aaaagtgctt
    11821 gatttttcat ttattattag tgattcgtca gggaaaccgg taaatggtag ttcattcatt
    11881 ttgaaacata agaaaaaaac acttactaga aatggtgtgc cgttttcagt acaaagcgac
    11941 catcatggga aagtcaagct ggatcaattg cctggtggaa cttatcgaat cgtttcagct
    12001 gatgatccta aagttgctgt ttcttttggt attagaaaag taaaacaaga gaagatatac
    12061 ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa atggttttta ttttaaaatc
    12121 aatgaataag taaggagaga aaaaatgatc cgcacaacaa caaaattgtc tgccaaagaa
    12181 ctggaatctg ttctatcgat ttggctgcat gctaacttgg attctcatga attcattcca
    12241 tcaacttatt gggagaatca agtagaatct gttaggaaag ctttaccaga tgcagtgatt
    12301 tacttagcag aaatagatca agaaattatt ggttttgctg gggtgattga tcagtttatc
    12361 gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa tcggtacgca actaatgaaa
    12421 aaaataaaag aaaccaataa aacactgact ttgacagtct atcaaaaaaa tcaatcagct
    12481 attcatttct atctttcaca aggttttaca attcaaacac agcaaaaaga tgaagcagta
    12541 aatgaggagg aacttgtgat gaaatggaat caaaacgctg atttttgatg aacagaataa
    12601 cgaaataatg ctcccagcct aattttttgc tgggagcatt atttcatttt gaagtcacta
    12661 atttaactgt gtgaatatgc agttgttaca atctttgata aacggtgttc aatcagctga
    12721 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttgtttctc
    12781 taatactcag gtcaaaacgc tgatttcaca acctcttgat aaacggtgtt caaacggctg
    12841 cttctcggta ataagcccac tagcctagaa aatatgaaaa gctattttct aactagcgtt
    12901 cttattactc aaagcataac gctgatttca caacctcttg ataaacggtg ttcaatcagc
    12961 tgaccctcgg tattagctca aaaaacggca aaacatgagg aactattttt ccgtttcttt
    13021 ctctaatact caggtcagaa cgctgatttc acaacctctc tttcacaacc ttattttact
    13081 cgtttataat tttcatcgaa gtattttccg ctacggatat catctggcat tccatcgtat
    13141 ggttcttcgc taataacatc ttcattgtct atacctgcat cacccatata agtgatcgta
    13201 gcaaattcag gaacaagtaa ttttggataa gtttcatatt tttcacgagc tgcttccatt
    13261 tctgcgatat gttcgttttg gaaagtaaca gtgatttctg gatgttcttc aacccattta
    13321 gggaaaaaga tcattccgtg aagtttcttt tcatttggca taaagtccaa tgccacgtct
    13381 gtccctgttg gctctgtcca agtgattttg aatacgccgt ccgtcaattt aacgatatca
    13441 gcttcttggt cttttaccca acggcctttg accattccgc catggatacg gtaatcaaca
    13501 gtatggtcat ttttggcata ccattcatat tcccaaccat tgtcatacgt atagataaag
    13561 tgagttccaa taaaatcttc caattcttta aattctttca tgtttaagca tctccttata
    13621 aattaaaaca tgtaataaac tacatgtttt cgattactca tttatgatag aatggattta
    13681 aagatttgtc aaggagggaa aactgatggc aagaaaaaat acacttcgat atattttgtt
    13741 agggttatta agtaagaaga agatgacagg atatgaattg aatcaatcgt tcaaaaatga
    13801 aataggagaa ttttggcaag caaaacatag tcagatttat cctgaacttg caaaaatgga
    13861 agagcaagga atcattcagc atcaagtaga gatcacaggt gaaaaattgg aaaagaaagt
    13921 ttatgaatta acagaagaag ggaaggaaca attggcagag tggattcata caccaaccaa
    13981 tgaactaccg gttaatcgag acgaatttgt tctgaaattg tatttcgtca aagacgtgaa
    14041 tgatccggca ttagtagaaa ttattcagca gcagcgtgac cttcatgaag aaaaacgact
    14101 tcatctatta gagcgaaaaa aaacgatctt tcctactgaa aaagaaaaac atgaaaatta
    14161 cggacactac ctgattttag cgcatgcgat caatcgggaa acagaatatg caaattggct
    14221 aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa aatatccgaa cagtgatgga
    14281 gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg gatattctta tgttcaaaca
    14341 ggcctttttt caagtattta tgtagcatct acttattaaa aagcttggta cttaggagat
    14401 tagtggcgtt tagagttctt ttaacaactg ttcgtagtca gctttttcat cgacaggaag
    14461 gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg atgtacatct tagcattctc
    14521 ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg tcgatcaatt cgatatcttt
    14581 gattgctttt gaaaaaactt tacggacatg gtgcattttc ttttggcgga tttctgcctt
    14641 ttctgcagct tttgacaaag taaatccgtc atctaaatat tttttgatca gttctacttt
    14701 tatgactgta ggtaaacgat attttctagg acaattcgca tcagcttcga tcgaacgaat
    14761 gaatcccttt tgttcccaat accttaattg acgtggagac acacctacga tatcacttaa
    14821 ttcactgatg ccaacaacca attgatcatt ttccaacaaa cgtttaaggg aagccccaac
    14881 catttttgtc actccttttg tcctcttgct tcccttaatt atatccgtat taacttatgt
    14941 gtcaagtagg ttgacaaata gcgaatttag gactaaagta ttctagtagt ttcagaaaaa
    15001 attcagaaag aaggagaaat atgagtaaca accaacccgt agatatttac ggaaaaccat
    15061 ataatcggac acttttagtt gttgtcttat taattggtac attttgtacc gttttgaatc
    15121 agaccttatt gacgacagct tttcctacat tgatgaaagc tttcgatatt tcagcgtctg
    15181 atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg gatcatgatc ccaataacag
    15241 catggctgat caataaattc agttcaagaa atttatatct ttctgccatg acgatctttt
    15301 tgatcggaac gattacttgc tttacagcac ctaattttag tacattacta atcggtcgct
    15361 tgatccaagc ttgcggcgtg ggagtatcga tgccattatt gcaaaatatc atgctatcta
    15421 ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc agggatcgtt attggacttg
    15481 ctcctgcttt aggccctaca ttatctggtt atatcattga taactaccat tggcgtgact
    15541 tgtttggtat ggtactgcca atcgttgttc ttgtcttggt tttagcattc ttcctaatga
    15601 aaagtgttat tccattgtca aatcctagca ttgatatttt atccgctatt ttatcaacga
    15661 ttggattcgg tagtttactt tacggatttt ctagtgtagg agatgacggc tggggaagct
    15721 tgaaagtaat tggtttctta gttgttggtg tgattttcat cgccttgttc tcatggcgtc
    15781 aattacattt ggaacatcca ttcttggaac ttcgtgtatt caaatcatct acattcacga
    15841 ttgcagctgt tttagctggt gtaacaaata tggcgatggt aggggcagaa atggtcgtac
    15901 cattgtatat tcaaaatatt cgtggagaat cagcattcca ttctggcttg atgttgttgc
    15961 cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg tgcgattttc gataaatacg
    16021 gcgcgaaacg tttagccatc acagggatgt tcattttgac agcggcaact gcaccatttg
    16081 ctttcttgac aaaaacgact ccagttttat atatcgtgat cctttatgcg atccgtatgt
    16141 ttgggatctc catggtcatg atgccggtaa cgacttctgg tatgaatgca ttgccaaata
    16201 acttgatcag tcatgggaca gcggtaaata atacattccg tcaaatcgca agctcgattg
    16261 gtacagctat tttgatcagt gtgttgacga atgtgacaaa agcaaatatc ccaagcaaat
    16321 ctgtattgga taatacgcca ttagcttata aagatcaggc aatcaatgcc acactgtcag
    16381 gttaccatgc cgcgtttatc gtagcgatca tctttgggat tcttggcttt attattgcct
    16441 ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg ggataatgca tgattattgg
    16501 agttttgatt gtcagcttgc ttgcttttgc tttaacgaac gtgttcgcta aaaaaacatg
    16561 gcaaaccttt ttatctttga ttttcggact aatcttcgtc gcttcactaa gtttgatcgt
    16621 cgctaatttg tccaatcact ttggaatgga aaaagtaacg gaaacaaaaa cagaaaaaat
    16681 tgtttccagt gctgattccc aaggagcaga tatgcttttg tataaagctt tagggaatgg
    16741 aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa gaaaaaccaa aagcaactgg
    16801 tacggataac gaaacaaata aagtagaaaa gacagacggc gatgctgaaa aagtgacgaa
    16861 aactacttat tgggaataca aaaacgacat gtataaattc tggttcaata ttgctgataa
    16921 caatcacgaa tatgacagcc gagtgaatac attcaaaatc ccagagacat gggtagaatt
    16981 aagtacggac caagcagcca agttagctga attagtgaag aaacagcaaa gtacgatgga
    17041 aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa gcaatgactg aaaatccaaa
    17101 aatgagcaaa gcagagcaag aacaacgtac gaaagcctta gctgctgaat tccaacaaca
    17161 agcattcgca aaattagtga aagaagcaaa aggcgaataa tattttgaac accatgtagg
    17221 aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca atcagataaa aatagcactc
    17281 catatttttc atctgattgg gcttagtgcc gtagatgcaa gcttttgaac accgtttaag
    17341 gaagaggttg tgagcctgcc gtttaactct gagtcacaag gagcaatttc aaaaaacagc
    17401 ttctcatgtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    17461 attcgaaaaa aggaactgcg acaagacttt tgtcacagtt ccttcttttt tttgctatgc
    17521 tatactagat gtaaaaaata ggagaaataa aatgagggaa atttattctt ttgaatcaaa
    17581 aatctatgct tccgaagtcg acaagggcgg ggcatatatt atttttccgt atgatataag
    17641 aaaagaattc ggtgctggaa gagttaaagt ccaagcaact tttgacaata tgccgtatga
    17701 aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt tcagtctgct acatccttgg
    17761 actacgaaaa gatattcgta aaaacatcgg aaaagaaatc ggtgatattg tagcagttac
    17821 agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt cacaacctct tgataaacgg
    17881 tgttcaaaag ccagtttctc ggtagtaagt caaatttatg caaaaatgtg gaaacccatg
    17941 tttacaaaat ttttcttact actcgaaacg aagcggcttt atcacaacct cttgataaac
    18001 ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg gcaaaatatg agaagctatt
    18061 tttccatttt tttctctcat actcaggtca taacgctgat ttcacaacct cttctttcat
    18121 aatgtttttt caccatcttt ataataaact ggttctttga tatggaaatc tattttttcc
    18181 aaccaatcta aaatcttgta acctaactct tctgacatcg gaaaagaggc gttgatttgt
    18241 gcattggaga tatggtcaac atggataccg gaagtgatcg tacaacttcc cggaacggca
    18301 gttttgatga tcacagctat tttttcggct aagacatcgt ctttatggaa ccgaccatcg
    18361 tggctaggga aacgtacagt ttgcatctct gtatcacgag aaaatgtagt gactgttccg
    18421 atatgtggtg tatctccgcc tgttaaagtg atcaataagt ctttaccgat tactttaaca
    18481 gcagcttgga tcgtgaaacc tagtttggtt acttcaacta tcgtttcttt ttccatatta
    18541 atcaccctgc caacgttttg aatgctcatt tacaattcca aattgatcca aaagtttcat
    18601 tgtatggtga ttaacaagat cttggatcgt ttgaggctga ttataaaaag caggaattgg
    18661 tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag ttttccaagt gaatggcatt
    18721 taatggcgtt tcacgaggaa caatgactaa ctttcgttgc tccttcattg ttacatcagc
    18781 agcacgtgca atcaggttgt cgcctaaacc aacagagata cctgcgactg ttttcatact
    18841 tgccggtacg atgatcattc catcggtcaa aaatgatccg ctggcgacag cagcacctaa
    18901 atctttgttt gaataatggt aatcaaacag gctttcgaat tcagttagtg ataagtcgga
    18961 ttccagcttt aagttctgtt ttgcccatgg actgatgatt ccgtgtgttt ctatcatcgg
    19021 atattctttt agtttcttca ctaggttgat ggcataaatg gtgcctgaag cgccacttac
    19081 accaatcacg atttttttct tccgttgtgc caattgttcc attttccgca ctccttttta
    19141 tgatgtcaaa acagttgcag atcattcatt atttcaaata tttttcccaa tcttctactt
    19201 cttggaattt tgcacgtaca aaatgttctt tcatatcgaa aggaactgta ccgtcaaaga
    19261 tagtttttgc actcatccca cggaaacgaa tagagccagg attatattct ggtaattctg
    19321 atgggtccaa tggatggttc ctcattcctg ggatcacgat gatatcttta tctccttgga
    19381 aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa aatatcaaca tcttcatcga
    19441 ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc aagaatagct gcttgacgct
    19501 gaataccttc atccgcttcg ttttctttac ggatttgcat gatggtcatc aatttaccac
    19561 cacctgcagg aggattgtag acatttacta cttttcctgg aatagcttta tcgaccaagg
    19621 caagaatact tgcttctgta ggaataccgg ccatagatac gtgttcttca ctgggaccaa
    19681 tcgttgtttg catgattgga ttgtcttttc ggtgagtaac tgctttgact ttcactactt
    19741 gtacagctgg attagcatcc ccagtatagc caggaaattc tggcattgct tttcctgagt
    19801 tcgtgttgat atcttcttgc attgtttcat ttggcatgat ttctgcttca atgatgaatt
    19861 cagcacgagc aatacccact tcatcgactg ctataccatc aacgatttga ataggttcgt
    19921 tacgcaaagc tcctgcacac catagttcat tgtagccaag tggagtggta ggtggttcga
    19981 aagtcgtgcc aatggcaatt gctggatcta aaccaatatt gatagtgata ggcattggtt
    20041 tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg gcgtccgcca ggcatgatgt
    20101 acataccgat cgtatcttta tcttctagaa ccatacgatg gatcgttaca tcgctcattg
    20161 ttttttctgg atctgatcct aagactacac ccattgtgat gtatggacca gcatcttcag
    20221 gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa gtctgcatcg gtagctagat
    20281 gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt cacaggtttg attggatttt
    20341 ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc atggtgcaaa atttcaccta
    20401 cacgtttacg actagccatt gttccgatgt tcacacgtgt gccggggaaa cctttgatat
    20461 tgttgaaagt catagctggt ccttcttgag tcggacgttt tactgtaccg ccagcaccga
    20521 tataacggta aacaccagat aattctgctt caggatcgat ttcaatatct gtttcgtgat
    20581 attgacctgg aagtgttttt agttcttcga tgacttttct taaatcatac ggttgatctg
    20641 tcatgagaga ttcctcctta gttgtcttta cctatctata gtaaaaggtg aaaaagagga
    20701 aaacaattca acttgttcgg taaagtattc tacaatggaa tggatgcgtt tttttgataa
    20761 tagaataaac tatgatatac ttatcactaa aagaaagcgc atacttagtg ttggaggaag
    20821 gaaagtgaaa aaaatgaagg agtcatttcc taaagcagta aaagtggaaa atatagcgaa
    20881 tattttgaaa gttacatttg aaaacggaga agtaaaatac gtgaaatctc attggacaga
    20941 agagataacc gatgccttgc aatttggaaa aaaaggaaga ggaaaaagaa aaaatcttct
    21001 cgcactttct cgtaatatgt ggattgggac tgaagtaacg attgaagcag atggaacagt
    21061 attcataaat ggaaaagaca ggtatacgcc agaagagttg tggtacaaag gaaagaaaag
    21121 tataccagaa ttataaaaaa taattaaaaa aaacagcgcg aaacaaggaa catattactt
    21181 cctcgtttct tgctgttttc tactatttca gcgctcttat gactcaaatc taaatgtggt
    21241 tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc ttttttcgct gccattcgtg
    21301 caggaagatg accgccaagc atcgtcaata tagtagagac gatcactaga ataatcgcat
    21361 ggacaggatt tagttgagca acatttttta gatccgtcat gttgtataag atgctattga
    21421 tagggaacgt agctagccat gcaataaaca cacctaatat cccagaagaa atacctaaaa
    21481 tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt acgtgcacct aaagctttta
    21541 gtacaccgat ttcttttgtc cgttcaatca cagatgtata agtaatgata ctgatcatga
    21601 tcatacttgt tactaaagag atccctgcaa aagcaataag gacataagtg atagcatcca
    21661 tcaatccgcc agtcagttca gtcatcgttc cagctaaatc agtataaatg attttatctt
    21721 catctgattt gcctttattg taatcatcta gataatccaa gatcttttct ttgtcttcaa
    21781 aattgttcgg ataaatcatg atacttgaag gcaagctgtc tccacctaaa taactaagca
    21841 atgtttgttt ggcattttcg tcaacttttt cagtagttaa gacatttaca ccactgtctc
    21901 tttgagcttt gacgatttct gattctttgt tttcgttgac gatttgagtc gttaattgat
    21961 cgctataagc gattcctgga gataagagat tcattgtaga agaagatttc acgcgtagga
    22021 tccccgagat cttcaattca tcagaagcat tctgatacat cgcatcataa tctgtattag
    22081 ggataaaatt cccagttgga agttttgtat agtaagtatt gttgtttact aatttgaatg
    22141 ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc tttcacatca aatccaagat
    22201 tcttcaatgc gttgatatta gtattattat tgccgtctac gatcagcacg acatctgttg
    22261 ctgaggctgg ataacttccg gcaagtaaag aataattgtc ttttaaaaag ttttctttgc
    22321 tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc tgtcattgct gacatcgtac
    22381 tagataaaga aagagattcc gtatcaggat tttgattcga gaagctgaca ggctgtactt
    22441 tgccatttac atcacgtaaa agattgattc ctgtagtacg ggtaaatccg atattattac
    22501 ttaagttagg atcgatgtct gttacgtaat cgacgtatgt ttgatcaatc ttattcgtgt
    22561 gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt tttgctgtct ggataatccg
    22621 ctttgcttgc acccaatccg gcatcgtcac gagtctggtt agtcgttacc ttagaaatag
    22681 taatcggaaa tttcgccatc gtttcagctt gcgtgttatc gatttgtttt tggaatccag
    22741 aagaaaggga cagaacgatc gcgattccga tgatcccaat actggaagca aaggaggtca
    22801 agaaagtacg tccttttttt gttcggatat tgttgaagga aagctttaac gctgtccaaa
    22861 aactcatctt tgttctgcgt aagttaaatt gatcatcttt tggtcgttcg atgtgaggat
    22921 gggaatctgt taagatctta ccatcagaaa attcaatgat ccgatctgcg tattgatggg
    22981 ccagttcagg attatgtgtg accatgatca ctaatttttc tttcgataac tcttggatca
    23041 attccatgat ctggatactt gtttctgtat ctaacgctcc ggttggttca tcgcacaaaa
    23101 ggatatctgg gtcattggct agtgctcgag cgatggccac tcgctgcatt tgtccaccag
    23161 ataactgatt tggcttttta tgcatatgat ctgtcaagcc gactcggtgc aatgcatctt
    23221 ctgcccgttt tcgtttttcg tctttagaaa caccacttaa tgtcatccca agttcaacgt
    23281 tttcaataat gcctagatga ccaatcaagt tgtaactttg aaaaatgaaa ccgatagagt
    23341 tgtttcggta ggcatcccag tcactgtcct taaagtcttt agtagacttt ccgttgatta
    23401 ccatatcacc ggaatcataa ttatctagtc caccaatcac attcagcata gtcgttttac
    23461 cagaaccact aggacctaaa atcgctacga attctttttg tctgaaagcg acagacacac
    23521 catccaatgc tttggtggtg gtttcgccga ctttataata tttttttata tcttttaatt
    23581 gaagcataat cacccttctc ccgtattgaa atattcggta tactaataag tatatcagaa
    23641 caatgatcgt agtatacgaa ataaatgtta actgaagatg aactacaaga aaaaatctag
    23701 aaatttttca aaaaacagag ggagatcaat gaatcgtatt ttagtagtag aagatgatcc
    23761 gcaattacag ttgctttacc gttctgtgtt ggaacgagcg ggcttttcag tcatcaccgc
    23821 attgaatgga gaggatgcgc tgaaaaaact agaagcgaca cgtatagaat taatcattac
    23881 agatatcatg atgccgaaaa tggatggcta tgacttgcta gactcattac gtagcaccag
    23941 atttgacaca cctgttctaa tgattacggc aaaggccgat tttgaggata agaaacgcgg
    24001 gttcaaatta ggagcagatg attatatgac aaaacccatc gatgtcaatg agatgatcct
    24061 acgtgtggaa gcattgcttc gacgttcaaa gatcaattac agcagtagct tgataatcgg
    24121 acaaacaaaa ttagagctag atgcttatac agtgttacaa gatcagcatt cgttgattct
    24181 gcctcaaaaa gaatttctgc ttctgtataa attactgtct tatcccaaca agatcttcac
    24241 tcgccaacag ttgatggatg atatttgggg attagatacg aatacagaag aacgaacgat
    24301 cgatgtacat atcaaacgtt taaggacccg ttttgaaaag acggaagatt ttcaaatcat
    24361 cacagtacgg ggactaggct ataaggcggt tatttaaaga tgaaaaaaaa gctattttca
    24421 caattatgga tctattttgc tagtattgtc ttcatttcga tcttagtgac actgctgtgt
    24481 tttctcggtt ttattttctt attgtctcga cattctatct cgcctgctga atcggctgga
    24541 aaaccaacgt tatacccgtt attcgtcttt gcggggttca gtatggtcgt gggtacagga
    24601 atatcgattt ttgttgggcg gcgtatcctg catccaatca gtgcgctagg gacaaatatg
    24661 agtctggtag caacgggaga tttttcgatt cgaatggatg aacagcagaa agtagcagaa
    24721 gtacagcaac tgtataaaga tttcaacgtt atggtccaag aactcaatag tatcgagaca
    24781 ctgaggaatg actttgtgtc cagtgtatcc catgaattca aaacaccact agcaacaatc
    24841 caaggatatg ttcaattgtt acaagcccca aatctctctg acgaagaacg ccaaatattt
    24901 ttatatcgga ttatcgaaag tatcacgcag ctttcacaat taacagagaa catcttaaaa
    24961 ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga aagagtatcg attagatgaa
    25021 caaatcagag aagtaatcgt ttttttgcag cctaaatggg agaaagaaca gctagaattg
    25081 gatatcgaac ttgctgcagt aaattacaca ggcaatgaag agtttttgta ccaagtgtgg
    25141 ctgaatatca tggataacgc cataaagtac aatcaaatca atggacagat tcatatcaaa
    25201 ctatttgaga cagccacaga gattggactg gaagtgaccg attcaggtgt aggaatgaat
    25261 gaagagacca gagatcgaat gtttgaaaaa ttttatcaag gagatacaag tcgtcaaatt
    25321 tctgggaatg ggctaggact ttcactggtt aaaaaaattc tagaactgca tgatggacgt
    25381 attgattata gcagtatcga aggagtagga acgacagcaa tgatacggtt aagtaaacaa
    25441 taggaaaaag aataaaaccc ctaaaaattc tattgaaggt ttttaggggt ttttagtcgt
    25501 ttttttgtga ttagggaaat gttcttacgg ttcttttggc ataaaaatcg ctgctttctt
    25561 attgaactac gagaaatgtt agaggtgtca ttgaaacaag gacatgatgc aagaaagaga
    25621 gcgaaagtca tgcgtcgctc tctttttttg ggtggataga tggacagaaa atgtagggct
    25681 tagttttctt cttgatttaa atactcccaa aatgtatctg aagccaaaga atgctcaccg
    25741 actttacgag taaatgcaag ataactctct gttgttggag aaatagggag aatcacagta
    25801 ttttctttta atagattctt gactgttttt tccataacaa tagcaatccc catgtcattt
    25861 gcaatcatat tgatgatcaa gtcgattctt gctcctttat aagagatttt tggttcaaat
    25921 ccagcttcgc gacacagatc tagtacaggt tgatacaagt tcgtagtggg gccaaggagc
    25981 aaaaagttct cctttttcag ttcgcttaga tccaatttct ttttactagc taaaggatga
    26041 tttttaggta gaactgctac aaaatcgtct gtttcagtag gcaaccactc aatcgaactt
    26101 ggtggggcct ggaaatttct agcaaagtgg atcgtgtcac tatgttcatt ggcgtcattg
    26161 taagttaatt cgacactttc cacttctttc aactggactg taaattcagg atggcgtttc
    26221 aaaaactcgg tgattttgga aaaaccttta taattgatca ttgtcggtat cgttaagata
    26281 tttaatgaaa gattgttgtc tttcgtatag gtttcaagca cttcttgcat agcatggtat
    26341 tgttctacga cattttttac atatgggagc aataagcttc cagcttcggt caattcgatt
    26401 tttctatggg cgcgttcgaa taatttgacg tctaattctg tttctaacga aagaatctgt
    26461 ttagaaatat ttccttgggt ggtgaattgt cgatcagcag ttttggtgaa attcattgtt
    26521 tcggctaaat caagaaaaac ttcgaattta tggatatcca tcatattcct cctacttatt
    26581 ccaataaaga atactttaac acaaaaaatg aattggtgta atcttctctg tctgttacaa
    26641 tctttcttgc agcgaaagca ataattacag aaatttttct gatttttttc gcttcgtaga
    26701 aaaagagagg agttttttga tgccaactgg agttttgatc aatgtaggtt ccgttttgct
    26761 tggcggacta attggcggat tagtaggaaa taaattaagt gaacatttta aagcacagct
    26821 gacgatggtt tttggtgtgt gttcgatggg gatgggaatc tattccattg cgccgatgaa
    26881 aaatatgccg gctgttattt ttgctttagt gataggaaca gcgattggtc tgattgttca
    26941 tttaggtaat gggataaata agggggctgc gctgatgcaa gtacctattt ctaaaatctt
    27001 ccctagtgaa aagcttggta tgacccacga tgaatttatc agcacattag tgacggtcat
    27061 tgttctattt tgtgcaagtg gtacggggat ctatggaagt ttagattctg ggatgacagg
    27121 agacagtacg attttgattt ctaaatctgt ccttgatttt ttcacagcag ctatttttgc
    27181 gtgtaattta gggtatgtcg tttctgtgat agcaattccc caattcatta ttttttatat
    27241 tttgttctta ttggcaaaat ttatttatcc attgactaca ccagacatga tcttagattt
    27301 taaagcttgc ggcggattct tgatggtcgc taccggtttt agaatgatca acgtgaaaat
    27361 gttcccagta gctgacatga tacctgcaat gatcgttatc atgccactga gctggatgtg
    27421 gacaaattgg attatgccac tgttataata aaaatcaaag agaagccatt ttttgctgac
    27481 taaaaaggat caaatctttt agtcagtttt tttgtatttg acaaactgat tttgaaacta
    27541 tattatatgt tgtataatat agattgaaat aaaatattac acttgagaat agaaggtgac
    27601 gtaatgcaca tccagattcc aactgttctt cttgatggta ctgtgctagc agcattaagc
    27661 aaggaagata tgtatgggta tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt
    27721 gaatctacac tatatccggt tttgagaaga ttgaaaaaag aaggatatct tgagacctat
    27781 gatgaacctt ttgaaggcag aaaccgtcga tattactcgt tgacaccaat gggaagtcgt
    27841 catttagaag aaattattga tgaatggatt gtttttcgag attctttaga ccggttattg
    27901 gaggagagca caaatgaatg aatatttgaa tgaagttgtg gatcatcttc aagaaattcc
    27961 tgaagacgat cgttttgatt tgatccagta ttatgaggaa tattttttag attctggaaa
    28021 aacattagat cagatcattg atgaatatgg tacaccaaag cagtttgcct taaaattaaa
    28081 aatcagttac ttttcagaag aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag
    28141 acaaattcgc ctgatttggt taatcattat cggtttattt gcttctcctg tcctcattcc
    28201 actagcactg gggtttatat tggttattgg gggactattg gttgctttac tagcgctcat
    28261 ttttagtatc tatgttttat gtatcagtat cttaggtgtt ggcctattca tgatcatctc
    28321 aggaatcagt gtgtttggac aatcgattct gagtggatta ttctttatcg gtgtcggtct
    28381 gttcgccaca ggaagcgcag tatttatttc gccactgttg ctgaaaggaa caaaagggct
    28441 tttttatgtc atgatgaact ttgttaaatg gatgggacga cgttttatta caaaaagaga
    28501 tctgcattca gcgggcgttt gaaggaggac acataaattg agattgaaac attttttgat
    28561 taccggtatt ggtctgatgc ttactggtgg cctcctagca ggtgcaactt atacattagg
    28621 cgctcaaaag agtctgactt gggataatgg tcctcggttg atcgatatgg ttactgagac
    28681 aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca gaaaatcaaa cagtttcggt
    28741 catacgcgga ttggattttg agataaaaac gacttatgat aagacagaaa agccagaaat
    28801 aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat
    28861 tagtttagaa gacacaagtg cacaaatcac tattgttgtc cctgcagatc atgaattatc
    28921 tgaattgaca atcgaaggga ataatagtgc aattgatatc aacgaagtat caatcaaaaa
    28981 agtgaatact aagacacatg atggctgggt atcgttcaat gaatttacag gaggaacaat
    29041 aactgcgaaa gatgaattag gctcacttcg attagatgag gccaatgtgg ataacttgat
    29101 cgtcacaggt gaaaacagca acttccatct agaagacagt ttgatcagca aagaaggaaa
    29161 agtcatccta aacggtggca atttatcttt gcatgagatg gaaaacagtg gctatactat
    29221 ccaaacgtct ggtgaatctt atattgaaaa agactaccag ccaataacaa atactttgtt
    29281 agaacaagga cagaaaaagc taaagatcac tgctaaagac acatcgatca atattacgac
    29341 ggatcaagag gacaatgaaa cagattgatc ctgtactcaa ctggatattt taagaaaaaa
    29401 cagcaaagat ccttccaatc tttgctgttt ttcatcgctt aacgcacata actatagtaa
    29461 aataagaaag aaaatatcta aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa
    29521 ggtcaaagag aaaagttaaa aacagaaatg gaaagattac atacttatat cactcaacta
    29581 agccaaacct tctatgatcc agacaaagaa aaagtgatgg ttaattaccc gaacaattca
    29641 gaaggtcgcc aattagagca agtgtaccat gaagttttca aacatttatt gacggtgaag
    29701 aaagagctgg attattattc tttaccgatt atcgatacag gaatattgaa gtatgatggg
    29761 aagaaggaac gatttatctt taaatctgtt cgggaagatt tgcctctttc agcagggatg
    29821 gatctagagg tcttggtaga agactatttt acagaagaaa agcactgggt tcgaacaaaa
    29881 ttggattatt taccgcaggc agccggaggg acacaggcaa ggggttggta catcactgaa
    29941 gacaaagagt tagaactaga aggtgtaatg gcgcgtattc gaaaaaaaat taaaaaagta
    30001 acattagata tttattgaag gataaaggag cgcaggaagt agcatgagag aaaaaagagc
    30061 attttatatc aatggttatg cagggattgt cggattggtc gtattagctt tagttggatt
    30121 gtttttcttc tatttaggta tgtggcaagc gaaagtttgg gcactttttt taagtatttt
    30181 tctttggttg atcacgttat tgttgttaag ttcggcaact gtagtcagtc ctaatcaagc
    30241 aaaagtaatt ttgttttttg gacaatattt aggtacgatt cgagaaaatg gctttttcct
    30301 tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa gttcggaatt tcaatagttc
    30361 tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa atctcagcag tagtagtctt
    30421 taaagtcgtt gatacagcaa aggcattatt cgatgtagcg tattatcaag attttgtaga
    30481 aatccaaagt gagacagcca ttcggcatat cgctagccaa tacccgtatg atacattcaa
    30541 tgagaatgat ttgacgcttc gaggaaacac gacggcagtg tctgacgagt tgcaaaaaga
    30601 gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc gaaacacgct tgaaccattt
    30661 agcctatgca acagaaatcg ctagtgccat gctccaacgt caacaagcaa aagccatttt
    30721 gtctgctcga caaaccattg ttgaaggagc tgtcacaatc actcaaatgg ccttagagca
    30781 aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg aaagtccaat tgatcaataa
    30841 tttacttgtc tcaatcatta cagacaaagg aacgcaacct gttatcaata caggcgacat
    30901 gaaagaccgt aagtgacata ctttttgtaa gataggaaga aggagtttag gacggattat
    30961 tgtcttgaac tcttttttta gttgttttaa agaaaatgac aaaattgaaa gaagaatgat
    31021 agttgattcg aaggcagaat tgttcgcttt tttctataat cagtatataa agtaagagag
    31081 aaggggaaaa agtgaaaaaa cttctgatcg gtctacttgt tttcatcgtt gtggtacttg
    31141 caggggtgaa aatcgcagat tacgtagtta tgggtggaga agaatactat gtacaaatta
    31201 cgacagatgg tacaaaaaca gtttcaaaat cagactctgg acaagaaaca gtcaactata
    31261 gatatatcct gcctggctat acgaaagagg gtaaggaaaa agaactggac ttcaagggac
    31321 aattgactcg accgcttcgt aaaggagcat atttaaaaat cacctggaac aaaaataaag
    31381 gtgtcacttc gtatgaagaa gtcgaaaaaa aagacgtgcc gaaaacagca ctggaaaaac
    31441 taattaaagg gtagggaaag aaatgaacaa aatagtcgaa gtacagaatg taacaaaagt
    31501 atacggaaaa aacaatgaga aaaagacgca agcattaagc ggcatcagct tcgatgtaga
    31561 aaaaggcgaa tttattggga tcatgggcgc ttctggttct ggtaagtcga cacttttgaa
    31621 tattttatcc acattagata aaccaacaga tgggcatatc cgaatcaatc aaaaagatgt
    31681 gaccacatta aaaggaaatc aactagcaga ttttcgtgcg aatgagattg gctttatttt
    31741 ccaagatttc aatttattag aaagcttgac agcacaagaa aatatcgctg ttcctctttc
    31801 tttacaaggc gtcagaccaa aagaaatcaa gcaacgtgtc caaaaaattg cagaacgttt
    31861 atccatctct catattttgg aaagctatcc ttctgagatt tccggtggac aaaaacaacg
    31921 ggtcgccgcc gcacgtgcat tgatcacaca accaacgatt ctattagggg atgagcctac
    31981 aggtgcgctg gattccaaaa gtgcccggga tcttttagat acgatggacg aattgaatac
    32041 aaaagatcat gtatcgattc ttcttgtgac gcatgatccg ttttctgcaa gttattgcca
    32101 acgtatttta ttcatcaagg atggcggtat ccatcaagaa gtgaaacgtg gagatcaaag
    32161 ccgtgattcc ttctatcgag aaatactatc tattcttgga aacttggaac agtaggggga
    32221 gattgactat gctttggaaa ttatcactta ctgggatcaa gagccgttta agagactact
    32281 tggtgttatt ctcaggatta gttatggcat ctgctatttt ttacatgttt gaatcaatgg
    32341 caagcaacga agcttttttg aaaagtaata cgatcgttgc ggctacagtt atcattttcc
    32401 gattcggttc tgttttacta gggatcatca cattcgttta tattttatat gcaaattcct
    32461 ttttgatgac gatgcggcaa aaagactatg caatgtttat gatgctggga gcaaaaggca
    32521 gaaaaatcgc acagatgatt ttttctgaaa cattcttagt agggatcgca gctactttag
    32581 tgggttcagc tgtcggtgta ggattgacaa gtatcgtcca ccgattatta gtggatcagt
    32641 tagatctcaa tatttcgcat tttacacctt ttagtattca cgggttgttg atcactgttt
    32701 tgttttttgc tgttttgttt ttattagctg ctgttgtcaa tgctttttcg attgtcaaaa
    32761 aaccgatttt agcattgatt cgtgcggatc agacaccaac aagaatcaag cagcataaat
    32821 tcttgttttt ccttgaagta gtactgggaa ttgcttttct aggctgcggt tactatatga
    32881 tgaaacacgt ggggacctat caagtctttg gtataggggt agccttagtt acgatcatat
    32941 taggaactta tttcattttc cattctgtga tcattttctt tttatcatta ctaaagaaat
    33001 cagaacagat ttcattgaaa aaattgaaca atttcacatt gtcacagtta aactttcgta
    33061 ttcgtgaata tacacaaatg ctctcaatgg tcgctatgct gtttgccttg gcactgggcg
    33121 ctttgacggt aggattaggt tttaaaaatg aaattgcaga cttgacagaa aacaattctg
    33181 gatatgatct agtgatgaat aatcctcggg cagaagatca gcaaaaaatc aaagaacttt
    33241 caccaacact ttctgcttct tatactcaaa aagaagatgc gggaacgatt tactataatg
    33301 caagtgaatt tgcggctcaa ccattgatca tgataaaaca tgaagatgga acaccgccga
    33361 agatcacgaa agaaaaagtt cctgctaaaa aaatgaatga actgactgta caagaggaac
    33421 ttcgaagcta tgaattgccg gaacagcaag aaaaagaaat caaggtagtc agtcaatcag
    33481 aatttgatca actgaacttg ccacaatcca ctttacagct agttcaggta aaagacttcc
    33541 aagcaaacct gaaaccaatc gaaacacttg ttgctcaaaa taaaatgaac aatccatctt
    33601 tgcaaaaagt agagtacagc aaccagaaat atgaaatgta tgaagtgtat aatggtctat
    33661 tctctggttt tgaatttatg ggattcttcc taggtttagc tttcttgacc atgcttgcta
    33721 gctgtttgat gttcaagatc ttgtcaggaa gcaagagtga cattgctcgt tatgaaatgc
    33781 ttgaaaagat aggaacacga cgcgggttgc ttaaacaatc gatccgtaga gaagtaggcg
    33841 tgttattctt agcaccagga atcttaggtg tagtccatgt cttattcgga ttacaaatgt
    33901 ttggtctatt catgcaaaat ccatacaaag atatttgggt tccgtttacg atcttcttcg
    33961 tattgtattt catctactat gtactgacga catggttata tactggaatc gtattgaaaa
    34021 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaagca aaaatagctc
    34081 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgtttg
    34141 ttcggaaaaa gaggttgtga caatattttt gtcacaacct ttttttcacg gtcttttatt
    34201 agaatatagt aaattttttc tcgttactca cggaataagc tgttaagaaa acggtttcca
    34261 tgtatactgg aatcagtgca aatgaaagaa aagagggtgc aaggaacatg gaaatcaatg
    34321 aacaaaattt tggcgaaaac tatcacttga ttacgatcac gaatcaaaat gggacatctt
    34381 tgtctgtcag tgatcttggg gcacgggttg ttcattttca aacaataatc gataatgaaa
    34441 aacgagagtt gatcttaggt tttgatgaag caaaagaata tctagaaaaa gacccttata
    34501 tcggtgcttc aatcggacgt atggctggac ggattacgaa tggcacattc aaattagatg
    34561 atgaaacgtt cgaagtaaaa acagacccgg acactggtca ttgtttacat ggtggtgcac
    34621 ctagctttga agccaagcag tggcattata cagtcatcaa tggtgaaaac gaagcatcgg
    34681 tcattttcta tcagactagc ccagatatgg gaaacggatt tcctggaact ttagatgtag
    34741 aagtacggta tacgctgacg aacgatgata catggcgagt gacggtccaa gggatcagtg
    34801 accgcaaaac agtattcaat cctacaaatc atgtctattt caatttaacc ggtgatccag
    34861 cacagtcaat cgacgaacat atactttggt tgaatagccg attatttgct acgctcaata
    34921 aagatacaac gcctacagga gaagtagttt cagtaagtgg cacagcattt gacttccaac
    34981 gtcctaaaaa attaggtgag gtctttgcca gtgattttcg ccaatcaatg atagtgggcg
    35041 gcatcgatca tccatttttg ttgaaaaaac aatctggttt actaaatgct gcggaattga
    35101 caagcccaga tgaaaaagta tcgatccaag taaaaacaga tgcttcagga atcgtgattt
    35161 ttacagcaaa ttttggagaa gatggaccgg aaatgcgcgg gaataaatta gtaaatcatg
    35221 gcggtatcac atttgagact caagaactgc caggagcgga gcgacttgaa agttttggag
    35281 atgtcactct ggaacctaac caagtacgcg agtttgtaac ggaatacaaa ataaatataa
    35341 agaaatagaa taaattaaaa agggagtgtg acagtgtcac atcccctttt taatttattc
    35401 tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa ggttagtaca
    35461 ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct tgccgtaaaa
    35521 ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt gttgacgcgt
    35581 tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca tttgattggc
    35641 acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt tgttgctgct
    35701 tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc gttcaatttt
    35761 tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc ttctttgtag
    35821 tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt cagtccattt
    35881 ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt acggaattgt
    35941 gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa ggtcacgctg
    36001 cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct tgataataag
    36061 ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc gtagattcct
    36121 tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg atcagttgtt
    36181 tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga gccgcctgtc
    36241 agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa atcaggcgtg
    36301 aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg gataaattgt
    36361 ccgttcccaa tatagatacc tacatggtga gtaacaccgg aagaaggtcc ccagaagacg
    36421 agatcgccag gttgggcctg acttactgga atctgcgtac ctgcatattg ctgttcgcct
    36481 gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt caaaccagaa
    36541 cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag atgcttgaat
    36601 gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac agcatcaatt
    36661 ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc gctttgattt
    36721 gctgtatttg gacgagatga agcattgttc gttccgccat tcgatgaacc attgtttgga
    36781 tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt tgtttgattc
    36841 gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga accattatta
    36901 ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt actagggact
    36961 tcttcactag gatcagttgg tttctctgtt gaagtatccg gtccagcttt aaattctgca
    37021 gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt tacttctttg
    37081 gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat agtgtaagtt
    37141 accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc caaaggttgc
    37201 tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa agaaggagca
    37261 attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc cggatcaatt
    37321 tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc ttctgtaaaa
    37381 gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt gactgtttga
    37441 tcagctattt gattatctac ggcaaatacg gtattggtcg acaaaacaga cggagcaatc
    37501 agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa ctagtccttt
    37561 tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg aacagataat
    37621 cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata aaacaatttg
    37681 taaataagca acgagcattc attcgttttt ctttgacgtt cattttattg catagaccac
    37741 ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa attataccaa
    37801 agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa aaagactgaa
    37861 atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa gacattaggt
    37921 cttcatcaaa cccgctaacg agagtagtga tcattgctac gactagacta acagccagtc
    37981 ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc agactaccaa
    38041 atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca gcagctgcac
    38101 caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa acagttggtt
    38161 cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa cgggtttttg
    38221 aacgtttact tcgcagaaat acacctaaag ctgcaccagc cattccaaaa ttggctgctc
    38281 ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc atgacagggt
    38341 ttacagccca ttggatacca aatattacca aaatattcca accaccagcg atgattgcgc
    38401 cactgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa ccttcgccaa
    38461 ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata actaatgaaa
    38521 gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg aatttttcaa
    38581 caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta tactgcatca
    38641 gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa agtaaagagg
    38701 tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca ttcactttaa
    38761 acgttttggc agcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg gcattcgacg
    38821 cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc gtagtcgcta
    38881 aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc ggcgtaaaga
    38941 tttccgatat caaaccaaat agtttttcga tcggatttag cttttctttc ggagctgaat
    39001 gatcgatttg acgcgtcaat tccttgtaag caggagccaa tccctgatcc atgattattt
    39061 ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt tttaattttt
    39121 ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca catggaagga
    39181 cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc tgctcggaca
    39241 gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac tcactaatat
    39301 aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt tattcaactc
    39361 ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca ataatttgat
    39421 tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa tcgattgtac
    39481 ctcaagttct tctaaaacgt tttctaaaac agataaaaaa cttggaagac cgtttgagac
    39541 agccaaatct attttttcag taccaaaggg gacaggcatg cctgcatccc cgatacttaa
    39601 tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga ttacttttcc
    39661 ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt gttctaaagc
    39721 ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag aagaggctag
    39781 atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg tgctgcttaa
    39841 tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt taaaagcagg
    39901 gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta gtgtaatgat
    39961 tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac tgttatgatc
    40021 tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa tgatatctgt
    40081 cttttcaagt agttcagtag gaatcgtttc acgagctggt gcaggattca ggatcgttgt
    40141 ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc tactttcaaa
    40201 ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga cttcttgttc
    40261 atggaaagca ttatttgccc ctgcatggat caagatacta ttttctcctg ctgcgtctac
    40321 cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag atgtatcgat
    40381 gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc caacgcctcc
    40441 aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttggttgg cacctttacc
    40501 tccgccagcg tgaaaaatct catgcgcatg catcgtttcg cctggcttgg gcatgttggt
    40561 taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt tcattttaca
    40621 aacctccaac tatctcattt attttgatgg gaaaattatt ttttaaaacg ttttagtaaa
    40681 acgttttaat agttcgataa aaatatagca caccttgaaa gctatttcaa gggtgcagtt
    40741 gaaaatcttt tttctaatcg tacaggtaat cttttttctt cccattcttt ttctgggaat
    40801 tggatccgat ctaacaataa cttggcagat gtttgtccca attcgaagat tggttgtgcg
    40861 atcgttgtca gtttcggctt gatatactcg cacatatcta tattatcata gccaataatg
    40921 ctgtagtctt cagggatgct ttttccagct tcctctaatc cgcgatataa tccaaaagct
    40981 aattcatcat ttaaagcaaa cacaccagtt gcagcagaat caagaagttc tgctgtgatc
    41041 tggtagccac cttgttttga gaactgtgta ggtaagagga tcagttggtc gtgagggatt
    41101 tggtaaacat caagagcaga tttaaacccc tcaatccgag cgtggacatt ttccggtgga
    41161 ttttcaggat agacaagggc gatcgtttga tgtcctagac taagcaagtg catgcccgct
    41221 aggtaaccac cgcgaaaatc gtctgttcgt acggcatcac taaatccttc agattttttc
    41281 tgatccaaaa caatgaaggg ccggccttgt tttttcaaat tttcgttgat tgcatctgta
    41341 gagacagcag aagtcgcgat aatgaatcca tccacacctc gacgtgtgag ctcggctaga
    41401 tattcgattt ctttttgatg atcagaatca gcattacata atatcgtgac gaaattctgc
    41461 ttatacaaga tatcctcaat tcctcgcatc agtgtactga agaatggatt cgtgatatca
    41521 ggaacgagta caccaattgt tttcgtttcc ttggtaatca tttgtctagc aaaatagtca
    41581 ggctgatagc ctaattcttc tttggcagct aaaactttct gaacagtttt cggactgaat
    41641 ttctccgtat ttccatttaa tatcaaggag acagttgtga ttgaaacgcc ggaaagttta
    41701 gcgacttctt tgattgtgat cttttttttc accatgttca tactcctatc taaatcctgt
    41761 ggttattttc ccatgaaatc aaaaagaagt aaatgaaaat cacgtgtgcc agtacttatt
    41821 tgctttgttg gatagcttcg attatttgtt tcgttgagcg tactcgtgca gttttcggaa
    41881 atatcttttt tatgaacaat tgatgggatt ctttgtcacg atcactgcaa gcatcttcag
    41941 caagcacgat atggtacccg tgttgaaagg catctaatgc agtagcatat acaccgtttg
    42001 ttgtcgctac accgcttaaa atgatagttt cgattccacg ccggcgaagt tgaagatcaa
    42061 gagaagtacc aaaaaaagca cttggattgt attttttgat agtcaacata ttatctgtat
    42121 ctttcaaaga atccttcaaa agtaaatcag tgaattctgg aggtacagaa gcagtcattc
    42181 cacccatatc agtttgttga ctcaaataag ggaaagtgga gatgtctacg ttcactaaaa
    42241 ctttcaaggc agcggtacgt ttcaatgttc ttactaacaa atcattgttt gtcagaatag
    42301 atgctgcagt gtgtggagag agtgtaccgt tatttacgat ctctttttgc atgtcgatcg
    42361 cgacaaaagc agtttttgat aaatcaaaaa tcatattttc ctttaaatca ttcatgatgt
    42421 tccttctttc aaataacaat gttttttatg taagtaaatc ataaatcgtt tatttgtcaa
    42481 ctaaattaaa ggattccttt ctttagtcta gagaaattca gtggtaaaat aaataagtgt
    42541 gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg aggaatcaga
    42601 gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga aacgaccatt
    42661 acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat tatacttggt
    42721 ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga tacacctgat
    42781 gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa acagcttact
    42841 gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt tcctaatcta
    42901 acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct tgatcctgta
    42961 gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc ccaattatca
    43021 ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc caaacttctt
    43081 ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt attgaaatta
    43141 ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca taattctgct
    43201 ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag gtctgttgaa
    43261 ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt tatgaaaaca
    43321 agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg attcattgca
    43381 atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac tgctggacct
    43441 gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag gactttctga tttgcaaatc
    43501 gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat cccagatgca
    43561 aaagtagaaa caggaaaaca gatctattat tccaatactg gcaaaaacga agtgattcaa
    43621 atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga tggaaaactc
    43681 ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg gtataaaatc
    43741 ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac catcacgggc
    43801 tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa cgtcggaaat
    43861 ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga tatttattct
    43921 gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa agcttacaaa
    43981 gataaaatgg agcaaaatca agaaaaggca gaaaagtatt tacaagatcg acccgaagaa
    44041 cgcttagaag agctgaaaaa atcggccaac gaagaattag aaccggcaca gcaaaaaata
    44101 gcggatggca aagcacaaac aacgcaagca agaacacggt tagaagatgc taaaaaacag
    44161 ttagcgcaac agtcagcaac tatccagcag cttcctgaag cgcaacgaac aatcgctgtt
    44221 caaacattac ctcaacaaga agctcaactt gctgaacaag aaaggcaatt agttgagaaa
    44281 gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa aattgaaaat
    44341 ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt tcaagaattt
    44401 ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt ctttttcttt
    44461 attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa ccgcagggaa
    44521 atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa atatgcaatc
    44581 tatgcaagtc tagcctcagg gataggtatt gttttaggaa cgatattagg aacgaatctt
    44641 ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag tgcgcttatt
    44701 ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc cgcatttggc
    44761 gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt gcttcaaccg
    44821 aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct gtggtcacgc
    44881 cttagcttta atcaaaaaat cagttaccga aatctcttcc gatataagtc aagaatgctt
    44941 atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt gggattgaaa
    45001 gattctctaa gttctgtttc agccaaacaa tttggaccga tcattgacta tcaagcaatc
    45061 gttacattgg attcatccac ggaagatagt cgtcaaaaag cagcggatat gctgcagaag
    45121 caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt gagaaaaaaa
    45181 ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga tttggatcgt
    45241 tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac aggaatcgct
    45301 attacgcaaa aagtagcaga actatttgac ttatctacag gagatcatat ttcgctttat
    45361 gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa ctatctaggt
    45421 agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc gatgatggat
    45481 aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat gctttccgag
    45541 aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca aatcgaaacg
    45601 caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt attatcagga
    45661 ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga gcgtgtacgc
    45721 gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat gtacattgtt
    45781 cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt aggatatata
    45841 ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc attagtgatc
    45901 acatggtggg cttatgtctt atcagcagga ttaactatcg tatttacggt tattgtgatg
    45961 attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa atcgaatgaa
    46021 taaaaacaga ggttgtgaaa tcagcgtttt gagctaataa cgagggtcag ctgctaattg
    46081 aacaccatga atcagaggtt gtaaaaaagc cgtcctgcat cgagcaaaaa gaatactcaa
    46141 ataaaattag ctcctcatgt tttacatttg agaagactga ttgccgtaga taaaagcttt
    46201 tgaacaccat ttatagaaga aacaagaata ttttgctatt ttaaa
//
LOCUS       DAZUKR010000006        44099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000006 DAZUKR010000000
VERSION     DAZUKR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.006"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..4487
                     /locus_tag="ACG89C_000343"
     CDS             129..4487
                     /locus_tag="ACG89C_000343"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS4898929.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            4632..5105
                     /gene="rimP"
                     /locus_tag="ACG89C_000344"
     CDS             4632..5105
                     /gene="rimP"
                     /locus_tag="ACG89C_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4898930.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            5128..6303
                     /gene="nusA"
                     /locus_tag="ACG89C_000345"
     CDS             5128..6303
                     /gene="nusA"
                     /locus_tag="ACG89C_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4898931.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            6325..6618
                     /locus_tag="ACG89C_000346"
     CDS             6325..6618
                     /locus_tag="ACG89C_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS4898932.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            6615..6926
                     /locus_tag="ACG89C_000347"
     CDS             6615..6926
                     /locus_tag="ACG89C_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS4898933.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            6939..9245
                     /gene="infB"
                     /locus_tag="ACG89C_000348"
     CDS             6939..9245
                     /gene="infB"
                     /locus_tag="ACG89C_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4898934.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            9272..9619
                     /gene="rbfA"
                     /locus_tag="ACG89C_000349"
     CDS             9272..9619
                     /gene="rbfA"
                     /locus_tag="ACG89C_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4898935.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(9776..10232)
                     /locus_tag="ACG89C_000350"
                     /pseudo
     CDS             complement(9776..10232)
                     /locus_tag="ACG89C_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10397..12759
                     /locus_tag="ACG89C_000351"
                     /pseudo
     CDS             10397..12759
                     /locus_tag="ACG89C_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            13127..14050
                     /gene="truB"
                     /locus_tag="ACG89C_000352"
     CDS             13127..14050
                     /gene="truB"
                     /locus_tag="ACG89C_000352"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4898936.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            14054..14995
                     /gene="ribF"
                     /locus_tag="ACG89C_000353"
     CDS             14054..14995
                     /gene="ribF"
                     /locus_tag="ACG89C_000353"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS4898937.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            15347..16132
                     /locus_tag="ACG89C_000354"
     CDS             15347..16132
                     /locus_tag="ACG89C_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4898938.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            16145..16996
                     /locus_tag="ACG89C_000355"
     CDS             16145..16996
                     /locus_tag="ACG89C_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4898939.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(17120..17572)
                     /locus_tag="ACG89C_000356"
     CDS             complement(17120..17572)
                     /locus_tag="ACG89C_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898940.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(18037..18405)
                     /locus_tag="ACG89C_000357"
     CDS             complement(18037..18405)
                     /locus_tag="ACG89C_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS4898941.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            18555..19037
                     /locus_tag="ACG89C_000358"
     CDS             18555..19037
                     /locus_tag="ACG89C_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS4898942.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            19074..20084
                     /locus_tag="ACG89C_000359"
     CDS             19074..20084
                     /locus_tag="ACG89C_000359"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS4898943.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(20199..20912)
                     /locus_tag="ACG89C_000360"
     CDS             complement(20199..20912)
                     /locus_tag="ACG89C_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898944.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(21085..21692)
                     /locus_tag="ACG89C_000361"
                     /pseudo
     CDS             complement(21085..21692)
                     /locus_tag="ACG89C_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            22176..22403
                     /locus_tag="ACG89C_000362"
     CDS             22176..22403
                     /locus_tag="ACG89C_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4898945.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22419..23585
                     /gene="hemW"
                     /locus_tag="ACG89C_000363"
     CDS             22419..23585
                     /gene="hemW"
                     /locus_tag="ACG89C_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4898946.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            23819..24862
                     /gene="hrcA"
                     /locus_tag="ACG89C_000364"
     CDS             23819..24862
                     /gene="hrcA"
                     /locus_tag="ACG89C_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS4898947.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            24956..25519
                     /gene="grpE"
                     /locus_tag="ACG89C_000365"
     CDS             24956..25519
                     /gene="grpE"
                     /locus_tag="ACG89C_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4898948.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            25570..27399
                     /gene="dnaK"
                     /locus_tag="ACG89C_000366"
     CDS             25570..27399
                     /gene="dnaK"
                     /locus_tag="ACG89C_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4898949.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            27550..28716
                     /gene="dnaJ"
                     /locus_tag="ACG89C_000367"
     CDS             27550..28716
                     /gene="dnaJ"
                     /locus_tag="ACG89C_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4898950.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(28798..29427)
                     /locus_tag="ACG89C_000368"
     CDS             complement(28798..29427)
                     /locus_tag="ACG89C_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898951.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            29439..29612
                     /locus_tag="ACG89C_000369"
     CDS             29439..29612
                     /locus_tag="ACG89C_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898952.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(29657..30394)
                     /locus_tag="ACG89C_000370"
     CDS             complement(29657..30394)
                     /locus_tag="ACG89C_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4898953.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(30396..31898)
                     /locus_tag="ACG89C_000371"
     CDS             complement(30396..31898)
                     /locus_tag="ACG89C_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4898954.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            32157..34151
                     /gene="uvrB"
                     /locus_tag="ACG89C_000372"
     CDS             32157..34151
                     /gene="uvrB"
                     /locus_tag="ACG89C_000372"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4898955.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            34161..36977
                     /gene="uvrA"
                     /locus_tag="ACG89C_000373"
     CDS             34161..36977
                     /gene="uvrA"
                     /locus_tag="ACG89C_000373"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4898956.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            37240..38505
                     /locus_tag="ACG89C_000374"
     CDS             37240..38505
                     /locus_tag="ACG89C_000374"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS4898957.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            38509..39240
                     /locus_tag="ACG89C_000375"
     CDS             38509..39240
                     /locus_tag="ACG89C_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS4898958.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            39375..40259
                     /gene="rapZ"
                     /locus_tag="ACG89C_000376"
     CDS             39375..40259
                     /gene="rapZ"
                     /locus_tag="ACG89C_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4898959.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            40256..41254
                     /gene="yvcK"
                     /locus_tag="ACG89C_000377"
     CDS             40256..41254
                     /gene="yvcK"
                     /locus_tag="ACG89C_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4898960.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            41288..42223
                     /gene="whiA"
                     /locus_tag="ACG89C_000378"
     CDS             41288..42223
                     /gene="whiA"
                     /locus_tag="ACG89C_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS4898961.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            42766..43416
                     /locus_tag="ACG89C_000379"
     CDS             42766..43416
                     /locus_tag="ACG89C_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898962.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            43603..>44099
                     /locus_tag="ACG89C_000380"
     CDS             43603..>44099
                     /locus_tag="ACG89C_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFS4898963.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGS"
BASE COUNT        14803 a         7595 c         9449 g        12252 t
ORIGIN      
        1 gatttgaatc gcagttccaa ttttttaatg taaaactcag aaaaaacgag tatactaaaa
       61 ggtactgaaa aagaagagga taagatgcga tcaattgcat tgtttctcat cagaaagggc
      121 gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa atccaactag
      181 aggaagcaga aaaaaatcat ccactgctta ctgctggtga aatggatagt gtagtggtcc
      241 atcgtaaatc gcgtttatgg gaatttacgc tgcatttttc caaaattttg cctattatgc
      301 tatatcggtc attgactcag catctgacta tcgcttttaa agatattgca caagtcaaac
      361 tgaatatctt agcagatgac cagacattcg atgaacagct cttgcaagat tactgggccc
      421 aagctttgga aaatcagcaa tgcgatacac cactagcaca gcaagtattg aaaacgcagg
      481 tacctgtgat aaaagatcgt aaagtcatct tgccaatcga cagtacagga gctatttctt
      541 atttgaagca acaatacttg ccattagtag aagagttgtt cgtcagttac ggattcccaa
      601 aatttcgtat cgaaccagaa gtggatgagc agcaagcgga acgagtattg aaactttttg
      661 aagaacgcaa acaagaacag gcagaagcct ttatgaagca agcagctgaa agtctagttg
      721 tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc
      781 acatccagct aggacgaaat attccagccg atgaaccaac gacaccaatg atcagtatcg
      841 ttgaagaaga acgaagagtc acacttgagg ggtacgtatt cgacaaagaa gtcagagaac
      901 tacgttcaaa acgcaagatc ttgactttga aaatcacaga ttatacttca tcatttatcg
      961 tcaaaaaatt ctcgaacggc gaaaaagatg aacaagtatt tgatgcgatc agtgtcggta
     1021 gctggttaaa ggtacgagga agtattcaag aagatacgtt tgtccgtgat ttggtcatga
     1081 atgcgcaaga tatcatagaa gtcaaacata cacctcgcaa agattatgct cctgaagggg
     1141 aaaaacgagt agagcttcat gttcatagca atatgagtac gatggatgca acaaacagca
     1201 tctctgatct tgtggcacaa gcagggaaat ggggacaccg agctatcgcc attacagacc
     1261 acggtggtgc gcaagccttt ccggaagcac atagtgctgg aaaaaaagca ggtgtgaaaa
     1321 tcttatacgg cgtggaagcg aatgtcgtag atgatggtgt cccaattgct tataatgatg
     1381 cacatgaagc attgagtgaa gccacatatg tcgtatttga cgtggaaacg acaggattgt
     1441 ctgcagtata tgacacgatc atcgagctag ccgcagtcaa aatgtataaa ggaaatgtga
     1501 tcgaaagctt tgatgaattt atcgatccag gacatccttt gtcacgaaca acggtagatc
     1561 tgactggtat cacggatgga atggttcgag gatcaaagtc ggaagaagaa gtattgcgga
     1621 tgttccttga attttcaaaa gatacaattt tggtagccca caacgcggct tttgatatgg
     1681 ggttcttgaa tactagctac gcaaggtatg gtattcctga agcggccaat ccagtcattg
     1741 atacgttgga attagctaga tatctgtatc ctcaattcaa acgtttcgga ttaggtgtgt
     1801 tatctaaaaa atttggtgtc agcttggaac agcatcaccg agcaatctat gatgctgaag
     1861 caactggtca cttagcatgg atctttgtga aagaggcaat ggacaatcat aacatgcttt
     1921 atcatgatca gctaaatgag catatcggag aaggagattc ttacaaaagg gctcgacctt
     1981 tccatgtaac gattttagca aaaaatcagg caggactgaa agatttgttc aaactgattt
     2041 caatgtcgaa tgtcgaatac ttcgaacgcg tcccacgaat accacgctcc caattgaaaa
     2101 agatgcgtga aaatctattg atcggctcag cttgtgataa gggagaaata ttcgaagcca
     2161 tgatgcaaaa aggggtagaa gaggcaagaa accgagcaaa attctacgat tatattgaag
     2221 ttatgccaaa agcggtctat gcgcctttga tcgagcaaga attggtaaaa aatgaacatg
     2281 atttggaaga aattatccaa aatttggtag aaatcggaaa aagtttggat aaaatcgttg
     2341 ttgcaacagg taatgtacat tatctgaacg aagaagatgc catttaccga aaaattttga
     2401 tcaattctat gggcggagcg aatccgttga atcgtcatag tttgccggat gtccatttcc
     2461 gaacaaccga tgagatgcta actgcatttc actttttagg ggaagaaaca gcgaaagaga
     2521 tcgtagtaga aaatacgaat aaaatcgcag atatctgcga agaagtgatt ccagtcaaag
     2581 atgaacttta tacaccgaag attccaggtt cagaagatga aatatcagaa ttgagttaca
     2641 caaaagcaaa acaaatgtac ggagatccgt tacctgagat cattcaaaaa cgattaaaaa
     2701 aagagttgaa ttctattaat ggtaatggct tctcggttat ttatctgatc gcccaaaaac
     2761 tagtgcataa aagtaatgaa gatggctatc tagtaggttc acgtggctct gttggctcta
     2821 gctttgttgc gacgatgaca ggaatcactg aagttaatcc gttagcccct cattattatt
     2881 gtccagagtg ccaatattcc gagttttttg aagatggtac gtatggttct ggatttgaca
     2941 tgccagaaaa acaatgtcca aaatgtggtg cacgcttaaa caaagacggt catgatattc
     3001 catttgagac atttttagga ttccatggag acaaagtgcc cgatattgat ttgaacttct
     3061 caggtgatta tcaagcagaa gcccataact atacgaaagt attgtttggc gaagactatg
     3121 tttaccgtgc cggaacgatt ggtacagtcg ccgacaaaac agcatatggc tatgtaaaag
     3181 gatacgaaag ggacaataat ctgcagtttc gaagtgcaga agtcgacaga ttagcaaaag
     3241 gcgctactgg agtcaaacgg acaaccggcc agcatccagg ggggatcatc gttattcccg
     3301 attatatgga tgtctatgat tttacgccga tccaatatcc agcagacgat caaaattcgg
     3361 aatggaaaac gactcatttt gattttcact cgattcacga taatgtattg aaattagata
     3421 ttctagggca tgatgaccca actgttattc ggatgcttca agatttatca gggatcgatc
     3481 cacaaacgat ccctacggat gacccggaag tgatgcggat atttgccggt cctgaagtac
     3541 ttggagtaag tcaggaacaa atttattcca aaacagggac attaggtatt ccagaattcg
     3601 gcactcgttt tgttcgaggg atgctagaag aaacgcatcc tacaactttt gcagaactac
     3661 tgcaaatttc cggactatct catggtacag acgtatggtt aggaaatgcg gaagaattga
     3721 ttcgacgagg agatgcgaca ctggcggaag taatcggctg tcgtgatgat attatggttt
     3781 atttgatcca tgctggatta gatagcggga tggcatttaa aatcatggaa actgttcgta
     3841 aaggacaatg gaacaagatc cctgatgaat tgcgagaaac ctatctatcg gcgatgaaag
     3901 aaaataatgt gcccgactgg tatatcgatt cttgttcaaa aatcaaatat atgtttccga
     3961 aagcccatgc tgcagcttac gtattgatgg ctcttcgtgt cgcttatttc aaggtttatt
     4021 tccctatttt gtattactgt gcatactttt ctgtacgtgc agacgacttt gatctagtct
     4081 ccatgtgcaa aggaaaagat gctgtgaaac aagcaatgaa agaaatcacg gacaaaggat
     4141 tagatgcttc cgtaaaagaa aagaatcaac tgacagtgct ggagttagcc aatgaaatgc
     4201 tggaacgagg attcaaattt ggaatgattg atttgtataa atcggatgcc gttaactttg
     4261 ttattgaagg tgatacgttg atcgcaccat ttagagctgt cccaagtttg ggaaccaacg
     4321 tggcaaaaca aatcgttgaa gcaagaaaag acggtccatt tttatccaaa gaagatcttg
     4381 ccacccgcgg taaagtttca aaaacattga tcgaatatat gaacgacaat ggcgtattga
     4441 aagatctacc agatgaaaat caattatcgt tattcgatat gctataagca tctgttttcc
     4501 tgtctgcttg taattttaag cagagtatgg tatagttctt ataggtaatg atactaactg
     4561 tgagtgagcg gatttttcgc tcactctttt taatggaatt acgcacttaa ttttttaagg
     4621 aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca ccgattttag
     4681 atgaacaaaa ttttgaactt gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct
     4741 tacgagtatt tattgacaaa gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg
     4801 agaaactgag tgaaaaactt gatgcagcag atccagatcc gattcctcaa gcttatttcc
     4861 tggaggtatc ctccccggga gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg
     4921 cacttggtga ttatgtccat gtctccttat accaaccagt agatggcgaa aaacaatatg
     4981 aaggatttct tcaatcattt gatgctgaac agcttacatt gaaaatacgc ataaaaacac
     5041 gagaaaaaga attgactttt gaccgaaaaa atattgcaaa agctcgctta gctatccaat
     5101 tttaatcacg gagaggaatc agaaaaaatg agtaaagaaa tgttaaatgc gttagacgct
     5161 ttagaagctg aaaaagggat ctcaaaagaa atcgtgatcg atgctttaga agcagcattg
     5221 gtttctgcat acaaacgtca ctatggccaa gcacaaaacg tcgaagtgga atttgatcag
     5281 aaaaaaggaa agatccatgt ctatgcggta aaagaagtaa cggaagaagt gatggattca
     5341 cagttggaag tctcattgaa agatgcactt ttaatcaatc cagcatatga gatcggtgat
     5401 acgatccgtt ttgaagtaac accaaaagat tttgggcgga tcgctgcgca aacggctaaa
     5461 caagttattt tacagcgtgt acgtgaagca gaaagaacga ttatttataa cgaattcagt
     5521 gcttatgaaa aagacatcat gcaaggaatc gtcgaacgcc aggataaacg ttatatttat
     5581 gtcaatcttg gtaaaattga ggcagttctt tctaaacaag atcaaatgcc aaatgaattt
     5641 tatcaaccac atgatcgaat caaagtttat gtttcccgag tagaaaatac gtctaaaggt
     5701 ccgcaagtat ttgttagccg cagccatcca gacctattgc gccgcttatt tgagcaagaa
     5761 gtaccagaag tctatgatgg aatcgttgaa attgtcagca tcgcccgtga agctggcgat
     5821 cgttcaaaag tggcagttcg ctcaaatgat ccaaatatcg atgcagtcgg tacatgtgtc
     5881 gggccaaagg gacaacgagt tcaagcaatc gtcaatgaat taaaaggcga aaacatggat
     5941 atcgtcgaat ggaatgaaga tccagctgta ttcattgcaa atgcattgaa tcctgcacaa
     6001 gtcatggatg tgatcttcga tgagacaaat ccaaaggcct gcacagttgt cgttccagac
     6061 taccaattat cgctagcaat cggtaaacgc ggacaaaacg cacgtttagc tgctaaattg
     6121 actaaccata aaatcgatat caaatcagaa tctgacatga cggaatttta tgaaaatcaa
     6181 gaacaacaaa aagaaacaga agaactgcat gatgaagcaa tcgttcagtc agatttaaca
     6241 gacgatgaat atgaaacaat tgcattcaac aatgaaacgg tcgaagaaaa accagaagct
     6301 taactttagt aaggaggcaa agagatgaaa caaaggaaaa ttcctttacg caaatccgtg
     6361 gtttcaggtg aaatgaaacc taaaaaagag atggtccgaa tcacccgttc aaaagaaggg
     6421 gaagtatcga tcgatccaac tggaaaaatg cctggacgag gagcgtatgt ctcacttgag
     6481 ccggaagaag tacaacaagc ttgggataaa aaaattctcg atcgagtact cgaaacaaca
     6541 ttaacagatg agttttatca agaacttctt gactatgtca ctcaccaaaa agcacgtaaa
     6601 gagttatttg gaaaatgaat acagttaatc ggcaaaaagc catgaatctc atcggtctag
     6661 cgatgcgcgc gggaaaacta atcacgggtg aagaactgac cattgctgaa atccgcaaac
     6721 aaaaagcaaa attggtgttt gtggcaaccg atgcaagcga gaatacaaga aaaaagataa
     6781 aagataagag ttcatattat gaagttcctt gctttgagct attttctgaa gcagaaatca
     6841 cgcagatgat cggaaaacct cgcaaggtat tcggaataat ggatgccgga tttgcgaaga
     6901 agaccaagga gctaattgaa ggttaggaag gtgattgcat gggcaacaaa agaatctatg
     6961 aattagcaaa agaactcaaa aaatctagta aagacgtcgt ggaaaaagca caacaacttg
     7021 gcatcgatgt gaaaaaccat atgggtgcga ttactagcaa tgatgagaag aaattgcaac
     7081 aagcgttcaa aaacccaagc acgaaacaac aagctgcaca acaagcaagc agaaaaccag
     7141 ccaatcaaca gccgaacgat caacgtaaaa aagaaacaac gaacaatcaa caaaaaaata
     7201 aaaataaccg cagttatcaa gatcgcggtc aagggagcgg tcaagtgaat caaggaaaaa
     7261 aaccaacaac aaaccaaaat aatacgggaa ataccagcag ccaaaatcgt caaggaacga
     7321 gccaaggcgg caatcaaaac cgtactaaca ataacaacaa accaaacaat aacaatcgcg
     7381 gcaaatttaa taataataac cgtaatcgtt ataataaaaa aggtaaaaaa ggcaaacagc
     7441 aacaatcaac taaaccagct gtaccaccac gcaaattccg tgaattgcca gatgtattag
     7501 agtatactga aggtatgaac gtagcagata ttgcgaaaaa aattcatcgt gagccagcag
     7561 aaatcattaa aaaattattt atgcttggtg tcatggtcaa tcaaaaccaa gcactggata
     7621 aagatacgat tgaactgtta gcgacggact atggcatgga accacaagaa aaagtccaag
     7681 ttgacattgc tgatatcgat aaattcttcg aggcagaaga agtcgatcca gataaattag
     7741 tctcacgtcc gccagtcgta acgattatgg gacacgtaga ccatgggaaa acaactttat
     7801 tggatacatt acgccattca cgtgtaacaa gcggcgaagc cggcggaatc acacagcata
     7861 tcggtgccta ccagatcgat attgatggta aaccaatcac tttcttggat acaccaggac
     7921 atgcggcctt tacaagtatg cgtgcacgtg gagcaagtat cacagatatc acgatcttag
     7981 ttgttgctgc agatgatggc gtcatgccac aaacagttga agcaatcaac catgccaaag
     8041 ctgcaggcgt tccaatcatc gtcgctgtaa ataaaattga taaaccaggt gcaaaccctc
     8101 aacatgttat gcaagaatta agtgagtatg agttaattcc tgaagcatgg ggtggagaaa
     8161 cgatctttgt tgagatctca gcgaaattcg gccaaaacat cgaagaattg ctggaaatga
     8221 tcctgttagt cgcagaagta gaagatttaa aagcagatcc aacacaacgc gcaatcggaa
     8281 cagtgatcga agcacgtctt gataaaggta aaggtcctgt gtcaacctta cttgtccaag
     8341 aaggaacact tcatgttggt gacccaatcg ttgtcggtaa tacttacgga cgtgtccgtg
     8401 taatgacaaa cgatcttggc cgtcgcgata aagaagcagg acctgcaacg ccagtagaaa
     8461 tcactggctt gaatgatgta cctcaagcag gtgaccgttt cgtagtcttc gaagacgaaa
     8521 agacagcacg tgcagcgggt gaagaacgcg cgaaacgtgc attattagaa aaccgtgctg
     8581 ccacaagccg tgtgactttg gataacttat ttgaaagcct gaaagaaggc gaattgaaag
     8641 aagttaacgt aatcatcaaa gcagatgtac aaggttcagc agaagcactg gctgcatcat
     8701 tgaagaagat tgatgtggaa ggtgtccgtg taaaaatcgt ccattcagct gtcggagcaa
     8761 tcaatgaaag tgatgtaacc ttagcagcag ccagcaacgc tattatcatc ggtttcaacg
     8821 ttcgtcctac accgcaggca aaagtgcaag ctgatactga agaagtcgat attcgtcttc
     8881 accgtatcat ctataaagcc atcgaagaaa tcgaaactgc gatgaaaggg atgctagacc
     8941 ctgaatttga agaaaaaatc accggtcaaa tgatcgttcg tgaaaccttc aaagtttcta
     9001 aagttggtac gattgcaggt gcctatgtaa cagaaggata tattcgtaga gatagcggtg
     9061 tccgtgtaat ccgtgacgga atcgtaatct atgaaggcca acttgcaagt ctgaaacgat
     9121 tcaaagatga cgtgaaagaa gtcaaaatgg gttatgaatg tggtgcaatg attgaaaaat
     9181 tcaatgacat caaagtagat gacgtaattg aaggatttat catggaagaa gtcaaaaatg
     9241 actaaaaact gataaaagga ggccaatcat tatggctaac taccgtgacc gccgagtcgg
     9301 tcaagaaatc ttaaaagaag tcaatgatat tttgcgcaaa aaggtccgtg accctcgtgt
     9361 ggagaacgta acgatcacag atgtgaaagt tacgggggac ttacagcaag caacgatcta
     9421 ttacagcatt ttatctgact tagcatcaga taagaaaaca gcacaagatg gattgaataa
     9481 agcatcagga ttgattcgtc gagagttagg gcacaatcta agtatttata agacacctga
     9541 attgttcttt gaacttgatg aatcagttgt ctatggcaac catatcgatg aattgcttcg
     9601 cgacttgaac aaagactaat caaaaactcc cgcaaatcct gtgaaatcag gggttgcggg
     9661 atttttttgt tttcattaag acgtattacc taagagttcc atgactttag tcttgcggcg
     9721 tgtcagctaa ccaaaaaaga gaaaaagaaa agtaaaaaat tcgactttct tttttctagt
     9781 agtatataaa tttttgcata tagataaaga tcaagatacc gataacggaa gctagattgt
     9841 aatctatttt agtgacagtc aggtatagcc aagtaactag aagatcagtg gaacctgcac
     9901 cataaaagtt tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg ttgaagttat
     9961 tgatatttcc gattacgccg cttcgatttg gtcacgatcc aaattttgga taatccctgt
    10021 agaaattagc atagtagctg ggataccaat tcacatattg acaaagataa cagagacttt
    10081 tacataaaac ggatcaatca ggaaaggaat tgctcgatcc agccccagtt ttccagcaat
    10141 gtgttaactg gcccagcgtc attcagcaaa ttatgcatga tgaggagcga aatgaactgc
    10201 ggaacagcca ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag
    10261 gaaaaatttc gtggtttttt atacctgttc atttggctag atataaaatc caaacaaatg
    10321 aacagaagtg cttattaggt ttcaattggg agtatctgcg ctagaataag agtaataatg
    10381 aaaaggagtg ttatgtatgg attattctag taaagaatac tttgataaaa tgacggcttg
    10441 gtggcgtgct gctaattatc tttctgttgg gcaactttat cttaaagata accccttact
    10501 gagacgtaca ctgaaacccg aagatgtcaa aaaacatcca atcggtcatt ggggaacgat
    10561 tcctggccaa aattttattt atgtccattt gaaccgagtc atcaataaat atgatttgaa
    10621 catgttttat attgaagggc caggacacgg cggacaagtc atggtatcga atgcttattt
    10681 agatggaagt tacacagaga tctatccaga agtaacggaa gatgagacag gaatgcaaaa
    10741 attattcaaa cgattttcat ttcctggagg cattgcttct catgcagcac cagaaacacc
    10801 aggttctatc catgaaggcg gagagcttgg atactctctt tcacatgcag tcggagctgt
    10861 attagacaat cctgaagtaa tctctgctgt cgtgattggt gatggtgaag cagaaaccgg
    10921 tcctttagct ggaagctggt tctcaaatgt ctttatcaat ccagtgatag atggagctgt
    10981 tttaccgatc cttcatttaa atggtgcaaa aatagccaac ccaacgatct tggcacgtaa
    11041 atcagatgga gaattagcaa attactttaa cggacttggc tgggaacctt tctttattga
    11101 aggaaatgat tcagaaaaat taaatcctgt tatggctgaa aaaatggatc aagcaatcga
    11161 gaaaattaaa tccatccaaa aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc
    11221 aaaatggcca gtattgatcg tacgcacacc aaaaggatgg accggtccaa aagaatggga
    11281 cggggaacca attgaaggaa ccttccgagc acaccaagta ccgattcctg tagatcaaga
    11341 acatatggat catgcagatg ctttactccg ttggctgaaa tcctatgaac cagaaaaatt
    11401 atttgatgca caaggacgta ttttagaaga aatcagagaa atcgcgccta ctggtgacca
    11461 aagaatggca aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc
    11521 agattggaaa aaatatactt tgcaatttga aaaaccaggc tccgttaaag cagaagatat
    11581 gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa atttccgtat
    11641 atttggacca gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg
    11701 ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt
    11761 catcgacagt caattatcgg aacaccaaga tgaagggttc ttagaagggt acgttctgac
    11821 tggacggcat ggttttttgc aagttacgaa tcattcttgc gagtggttga ctctatgttg
    11881 actcagcact tcaaatggat gcggaaatca catgaccttt cttggcgaaa tgattatcct
    11941 tcgctaaatc tcattgcaag ctcaacagtc ttccagcaag atcacaatgg atatagccac
    12001 caagatccag gaatattgac acatttggca gaaaagaagg cggagttcat ccgtgaatat
    12061 ctgcctgcgg atgcaaatac attgctcgca gtcatggata aagcattccg ctcgtcagaa
    12121 aaaatcaatt tgatcatctc ttccaaacat ccgcgtgctc aattttactc agcagaagaa
    12181 gcagccgtct tagtgaatga aggcttgaaa atcattgatt gggcttcgac agcaaaagag
    12241 gaggaacctg aattggtcat tgctgctgca ggaacggaat ctaacttaga agcattagca
    12301 gctgtaactt tgctgctgga agaattccca aaactaaaaa ttcggttcat caatgtcgta
    12361 gatttattga aattacgtca tccaagtcaa gatccgcgtg ggttgagcga tgaagaattt
    12421 gatcaatatt ttacaaaaga caaaccgatc ttatttgctt tccacggtta tgaaacattg
    12481 gtccgcacga ttttctttga tcgtcataac catcatttaa tgattcatgg ctacaaagaa
    12541 aatggagata tcacgacacc atttgacatg cgtgtagtaa atgaacttga tcgttatcat
    12601 ttggcaaaag acgcagcttt aaaaatcaaa ggaagtcaag cagaagattt tgcgaaaaaa
    12661 atggatcaga aattacaaga acatcaaaac tatattcgtg aaaatggtat tgatctgcca
    12721 gaagtattgg attggaaatg gaaaaatctt gaccaataaa atgataaaat cgatcccgca
    12781 aaatagaatt ttttacattg attaagaaac tgaaaaaata aataaaaaaa acggaaaaaa
    12841 gactggaaga atggccagtc ttttttttcg tttttgggaa gtgaaaacca aactatttac
    12901 ctgtaagcca cccataaatc caatcaaatg gtgcaaagaa aaaatcttcc attctattcc
    12961 ctcctttaaa aaagcataac atgatttcta tttacgtgag catgaatttt gttacgtttt
    13021 tattgccgaa aaataaaaac cgttcattat gatgtcaaat tcgtcagtaa ttgatgcaat
    13081 gtgttataat aattcagtta aatcaaaaat gaaatggaga aaagacatgg acgggttatt
    13141 acctttatgg aaagaacgcg gaatgacaag tcatgattgc gtattcaaat taagaaaaat
    13201 cttacatacg aaaaaaatcg gacatggggg tacgcttgac ccagacgtag acggtgtgtt
    13261 gcccatttgt attggaaaag ccacaaaagt tatcgaatat ttgacagatt ctgggaaaat
    13321 ctatgtaggc gagataactc taggtttcag tacaacgacg gaagatcgat ccggagaacg
    13381 ggtagaaaca aaaccagtag atatgtcttt atctgaaaaa aaaatagatg aagtgatggc
    13441 ttcatttatt ggtgagatca cccagatacc accaatgtac tcagcggtaa aagtcaacgg
    13501 taagcggtta tatgaatatg cccgcaataa tgaaacagtg gagcgtcctg ttagaaaggc
    13561 tcagatttac cgttttatcc ggacaagtga attgacaaga gacgaagtga caggaaccat
    13621 ttcttggcgc ttcgaagtag aatgcgggaa aggaacctat gtccgcacat tagctgtaga
    13681 tacaggcaag aaacttggtt accctgcgca catgtctgat ctgacccgaa cagcaagtgc
    13741 cggtatgtcc cagaaggatg ctctgacatt agaagaagta gctcaagctg tgaaaaatgg
    13801 gacaatcgat gattgtttgc tgccaataga aaaaggagtg gaacggttca aacgagtaga
    13861 tattactgat gaagtatggc agaaagtaaa aaacggtatg agacttgatt atactgtatt
    13921 tgcgctccca gaaatgccgt cagaagaaat tgctttgttt tataaaggaa aagttgtcag
    13981 tatttatcag ccaaacccga aagaaaaaaa caaattaaaa cctagtaaag tattgagaaa
    14041 tgaggaataa cgaatgaata tcatcaagat cagacatcct taccaatcag aacaaattcc
    14101 agaagacgat gtcgttttag tgctgggatt tttcgacggc gtgcatatcg gacaccagaa
    14161 agtcataaag acaggtatgg aaattgcaca aaaagaagga ttgaaattag cattgatgac
    14221 tttcaatcag catccatcta ttgtctttaa aaagatcgat cctagcagcg tgaaatattt
    14281 gactagcctt aagcaaaaag aagaaaaaat ggccgctttg ggtatagatt atttatatga
    14341 agttgatttt acttcttctt ttgctcattt agcaccgcag gaatttgttg atcaatacat
    14401 cgtagggtta catgcaaaat atgctgtttc cggttttgat tatacgtatg gaccaaaaga
    14461 cattgcagat gtcgctcatt ttcctgaata tgccaaagga agattcaaaa tcgtcacagt
    14521 ttctaaagaa gaagaacaag gtgaaaaaat cagctctacc cgtattcgtg gattactaga
    14581 cagcggacaa gtaaaagaag cagcaaaact attaggttgc acatatgagg tagaaggggt
    14641 agttgtccat ggcgatgcac gaggcagact actaggattc cctacagcaa atgtcaaagt
    14701 aaagagcacg gttcatttgc ctaaagaagg tgtttatgtg tcggaaataa aaattgggga
    14761 aacatggtat ccagccatgg gttcaattgg acataatgat acattcggtg aaggacggga
    14821 gctgactgta gagttgtata ttttggactt taatcaagat atctatggtg aacatgtcgc
    14881 ggtacgctgg aatcaatttt taagagatca agtgaaattt tctagtgtag aggaattgat
    14941 tgaacaactc aaggaggatg aacaagcaac agctgaatat ttttctcagc aataaaacat
    15001 tatgcataaa atctcttgtt tatgtataac tagcaattac aataaaatgg gtgataaata
    15061 agcccaggaa aggggctgtg acaaaagtct tgtcacagcc tttttttcca aataaatggt
    15121 ttttcagaag tagcttcttc ggaaataagc cgaaatttct tgaagctgac cacttctgtc
    15181 acaacctctt tctttataga aataaaagga cactagcaat tcatgaaaac gattgctaga
    15241 gcgtatcatt tgctttagac tatatatctt ggtgtacact cactttaagg cggaaataaa
    15301 agttatccgt aataacaggt ttaggtatga aaaaggagtg ggtactatgt acaaggaatt
    15361 agaaaacaaa gtcgcagtag taactggtgg ctcaaagggg attggaacag cgattgctca
    15421 acgattcggg aaagaaaaaa tgaatgtagt tgtcaactat aactcggata aagaaggagc
    15481 agaaaaagca gtcaaagcaa tcgaagaggc aggcggaaaa gcagtctctg tacaagcaga
    15541 tgtcagttct gaagaaggaa tacagcgttt acttgatacg gctttagaaa attttgggac
    15601 actggacgtt tgggttaata atgctgggat ggaaaatcag catccaaccc atgaacttag
    15661 ccttgaagat tgggaaagag tgttaaaagt caatttgact ggcgttttta tcggaacgaa
    15721 agcggcttta aattacttta cagagaataa taaaaaagga aatatcatca atatgtcttc
    15781 tgttcatgaa cagatcccat ggccaacgtt tgctcattac gctgcttcaa aaggcggggt
    15841 aaaattattt accgaaacgg tagcaatgga gtatgcaccg aagggaatca gaatcaatag
    15901 tataggccct ggtgctatcc gtacacctat taatgcggca aaatttgatg atcctgaaca
    15961 aaaagaactc ttggaaagta tgatcccact aggacgcgta ggtgaaccaa aagaaatagc
    16021 ggcgacagct gcatggttgg cttcagacga atccagttat gttacaggta ttacactatt
    16081 tgcagatggt gggatgactt tatatccttc tttccaaggt ggaaaaggat aagggtgaat
    16141 aaaaatgggt ttattgattg cattgatccc ggcagttggc tgggggatcc agccactgat
    16201 tttgaaaaag attggtggca gaccaacaaa tgagatatta ggaactggta ttggagcggt
    16261 gcttgtcggc ttactcgttc aattattcgc ttctccagct ggtatttcag tgactacatt
    16321 tttgattagt ttactttctg gtgcattttg ggttattgga caaacaggac agtatacatc
    16381 tttcaacttg attggtgttt ctaaaacgat gccaatttcg actgcattac agttagtcgg
    16441 aacctcgttg attggtgtct tcgcttttgg agaatggtct ggaagtatgg ggaaaatcat
    16501 tggagcagca gcaatcattc ttcttgtatt tggttctgct ttaacggcaa tcagtgatga
    16561 tcctggaaaa aaaggcggta tcacaaaagg aattagtcta ttagcatcaa caagtattgg
    16621 ttattgggta tacagtgcat tgcctaaact agttgatgca agcggacttt ccattttctt
    16681 tccgcaaatg atcggtgtct ttttaggggc agtgatttat gtgattttcc gtaaaccaac
    16741 cgcattttct gaagacaaga gttggaaagc aacgatcgtt ggagccatct ttagtgtgtc
    16801 agcattggct tatatttttt cagcaaatga aaatggggtt gctactgcat atatcattac
    16861 acagctaaac gtagttatct ctacattagg cggtatgtac atcctgcatg aaaataagac
    16921 accaaaagaa aaaaaattaa cgattatcgg attattgctg atcgtagtag gaagcatggt
    16981 cacggtcttt atttgaggat aaagatggta aaatttcatt tattaaaaaa atatttgttt
    17041 gaaccaaaca gtaagtactt ataaaaaaat agcgctagaa tttccaacat ctctggaatt
    17101 tctagcgctt tttaattgtt taagatacaa gggaaatatc tgtctggatc aataattttt
    17161 cattgatcca ttgactaaag tttacgacat aattgaagtt accaccgatt agtatctccg
    17221 catgattgtc tttataaaga tcgtattgta aaagacggtc ttttttattt gcgtaaacat
    17281 cgttatggta ctggactaat tgttctcgga attgattgat tgctgtaaaa tcacgatatt
    17341 gaaggttgaa acgcatcgtg attggtgcat ttagcatgaa tcgctgcatt tcttttgctg
    17401 cttcctctgc ttgatctccg tataggaact caaaaagatt acgttcagaa aatttccatt
    17461 gcccgccaac ctttgttcct tttagttttc cttcatgaag ataatttctg atagaacgtg
    17521 tagtaacacc taattgttcg gctaattcat ttactgagta aaaatctttc aatgttcacc
    17581 ctcctatgtg aatggttcta ctgacttagt tgatatttgc ttaatcacga aatgtagaaa
    17641 gaacgatttt aattaaggtc attgtaaaaa tcaagtcaga aaattctaac ttttatttct
    17701 ctaaagaaat tcgttctctc ctaaagtgat attatattta aatatacctg aaaataatgg
    17761 gcagtattat gaaagatcac tgatattttc tcgtattttt tccttattca aaagttttgc
    17821 aggaaaattg ctgaaaaaat aattttacta tgtagaaaat tcaaagccat tcaatcagtg
    17881 atttttttca gcacaagaaa gactccttat aaaaatagca gatactttgt caattaagtg
    17941 tttaaccagg gatgataaaa aggataggat tgaacaaaac ctgtataagg tgatgaatag
    18001 tttcctcgcc agtaaaaggt ttattcgtat aaagctttat cgccaaaatt tatcatgacc
    18061 ttttttatct tcaatattaa atgaaatcaa ttgttcaatc aattcccagt tgattacact
    18121 tttccaagga actttgatga cgttatccgt atatacgtag cctgcttctt ggatattttt
    18181 ttcaagggca cgaatcgctg ctttttccgg tgcgatagaa aaatgatttt tagcaaaact
    18241 aaatgcaata ataaaggtac catggtcagt aaacataggt tggttccatt ttacagttgt
    18301 gtctagctgg ggatacgttt ctgttatctt agcaaaaagg tctttgatct tttgttgatg
    18361 ttctttttct ttgatttgat tgatgatcgg ctgaaatttt tccattctaa aacgcctcca
    18421 ttaagtggtt tgatttattt taccagaaaa acaatagtta tgtataaaaa acgaacaaaa
    18481 cacaagtaat gttgtaaccg ctatatttat atgataagat gtctcaaata ataaaataga
    18541 aaagagacat aaaaatgatt gacttggcaa agaaattctt acacgaaaat atatattttg
    18601 agttgtatca tcatcgagcc atttatacaa atgaagatgc tttggtaata aagaaagaac
    18661 aaggatttac aggtacagag accaaaagct tattcttaaa agataaaaaa gataataact
    18721 acgtatatct gacttttact acgaaacaaa cggatttcaa agcattaggg aagatcgtcg
    18781 gtaaaaggct ttctattgtt tcatcagaac gcatggaaga acaaacggga caaaagcagg
    18841 gcgctgtttc tccgtttggt tatgaaaaac agattccgat tattattgat gaagagctct
    18901 tatcaaatga aaaacttgtt tttgcaccag gtagaccaga tcaaacgatg gtcatccaaa
    18961 cggttgactt aaacaaaatc atccagctac ttgagatgcc gatatttttg atgccggcaa
    19021 acgaaaaaaa caattaacca agtaaaatag tgcatacttg gaggtgaagc aacatgataa
    19081 gttatccatt attagaaaga aatgcaacga ttggggttac tgcaccatcc tcaggcgtat
    19141 cagaggaaca ccattcgtta ctcgaatcag ctttaaagag aatggaagaa aaatctttcc
    19201 aagtagttac tggaaagacg ctttggacgc aagataagga aaaatcaggt ccagcaagaa
    19261 aacgagcaga tgaattcaat caaatgatgc aagatgaaaa tatccaaatg atctttccga
    19321 cttggggagg agaattattg attgagattc tcgattttgt cgattttgat gctctacaac
    19381 caaaatggat agtaggttat tcagatatta gtacgttgct acttgctgtc acattaaaaa
    19441 cagggatagc cactgcacat ggaactaacc ttattgattt aagaggagag tacttggatg
    19501 aaacgacagc aatgtgggaa agtgtactga aaacaagaga aggagaatcg attctacagc
    19561 aatcttctga aaagtatcaa aaagtgtggc cccatgagca ttcatcatct tctgttttcc
    19621 atctcactga accaacaaat tggcaatcag tcagcggaga tgcagtaagg atgaagggac
    19681 gattattagg tggttgtatt gatacgatca ggcatttaat cggtactcct tatggagatg
    19741 tccaatcttt taaaagaaag tataatgatg gaaagccgat cttgtggttt ctagaaaact
    19801 gtgagctatc gtcagttgat ttaagaagat cacttgttca gatgaaactg gcaggctggt
    19861 ttgatggcag ctcggggatc atgttcggta gaagtgcggc agaggatttg ttagattata
    19921 aaatgaagga tgtatacgaa gatctgtcaa aggaattaaa actaccgatc ttgtatgata
    19981 ttgattgtgg tcatgtaccg ccacaactga cattgattaa tggtgcctat gcagaagtag
    20041 cggtagaaga tagaaaagcg actgttttgc aaacgtttat ttaaaaaatt ttttcctgcc
    20101 ttggagtcta tcaaagacca ataaatctaa atagaaaaga aagtgaaaga agctgacaaa
    20161 atgctgtcag cttctttttg tataacatat tagaaagtct aattatctat aactgtcaca
    20221 tcgattaact gtttcacgtc attgggattt cctgtaggaa tagctactac agatgcatta
    20281 tcaatgattt cttgagttgt ttctctaatg aacgctggat aagatgtttg atctgcaggc
    20341 atttgccaat gtgaaggaac tggataacta gtgattgtcc cgtcatggtt acttgaatac
    20401 actaaaactc cctggtaact gtatttccca gtgagcataa ccacatcttt cggatagact
    20461 gcactccctg catcataagg ggcgataggt gttcctgcag gataatgaat aacatgtaat
    20521 tcgaaatcac ccgatgtatc tctaaaagta ttacctctaa tagctagcca aacacgtgcg
    20581 tactcaattt gtagatcaga atatcctgct aacggatcta ttgactgaga agactgtgag
    20641 gaggttgtgt ctattgttga agaagaaata tttgtctcaa ctgtactact agatgtagaa
    20701 gaagtattgg gctctgagac tttactgctt gttgaagatg tattcgtttc agtagaagct
    20761 gatttctgag tacttgattt ttcagctgaa ctggtttgtc tgattgaact tttgtctgtg
    20821 gtcgcagatg gatcactatt ttctttacta ggagacgtac atcctacaag gaataaagag
    20881 accaacaata aaataaaacc taactttttc atataaaata cctcattatt tttttaatac
    20941 cagtatagca taatcctgaa tttttgaatc cgtaaaacct tgtctatgaa tataaataga
    21001 gtgtatcttc tttgataaat gtcagaaaaa gtgtgacaaa taatcatcag taaaattact
    21061 tgtcacacta ttgataagga gtatttaatt gaagagaatg attaggagaa aggatctccg
    21121 tactttttat agtaatcata gaatccaagt ttgtatagac gaatcgtttt ttctcttatt
    21181 tcttctgttt caaaatggac tgcttgaaga atcaagtctg tgaattctat aggagctgtt
    21241 ccattcgcag taatcaaatt acgatctttc acagcttgtt gttctataaa atcttcttta
    21301 ttctgataat tttccgattc taaccaagga aattgcgcat ttccggtgtg tttgtaacct
    21361 tgcaataatc cgctctgagc taaataatca actgccccac aaatagcagc tactggtacg
    21421 tctgttttta acaatttatc gattatttga tataaatgat gattttttat gtcccatgaa
    21481 ttgccaccga tcataataaa aaggtcaact ttttcaggta tttgttcgat taagtaatcg
    21541 atttttgttt ttaatcctcc aatggaagtg acctctgatt ggatagaagc agttttgttt
    21601 tgccatttct cattttggtt tagtgtgcta gtaatataag caccttccca atcggcatat
    21661 tcctctaata gaaacgtaat cgcagttttc attttcaagc tctcctttac tatctataac
    21721 aaggacaaca gttttaacct acagacattc attaaaattg ggtagcgaca gcgaaaacaa
    21781 caattcttaa gttttagaca gagtatacag atagtcaact taattaaata cgaactaaat
    21841 aaaaatgaca ggtaatcatt tttgtttacc agcattataa cattttgcat acatggatga
    21901 caaaagaacc aattcccact tgcttgagta caatattaaa agaaactgga aggcaaaaag
    21961 taattataga aacgaaaaat aaatcatatc gggcactcag acatgaaaac aaccctagag
    22021 atatataact aggtaaccag tttaatgaaa gaaaaaatca tccaagaagt cgattcttgg
    22081 attttttagt ggatttgttc gccaaatacg tttagtatgc gtatatgaga ggttgtaagc
    22141 aaagcgttgt ggtctgagca cttaaggagg aatctgtgaa aatagctttt catatttttg
    22201 cagattttga cttaatgccg aaggtcagct ttgcgattac cgtttatcga gaggctgtaa
    22261 aagcaccgtg tagctccgat gacaataagc aagcattttt agagctaggt ttccttgatt
    22321 ctaaatatgc ggcttatttc aatgagttgg tgcttgaacg cgattttaag aaggtcgaaa
    22381 acaggcacga cttttcgacg taaggagaaa gaatttcaat gatagaaaca ataaatcccg
    22441 cagaaaacaa ggtttctgcg tacattcata tcccattttg cgagcatatc tgctattact
    22501 gtgattttaa taaagttttt ttagaagggc agccagtaga tgagtatgtg gaaatgctgc
    22561 tgaatgaaat gaaaataatg ctagaaagtc atccgacagc gaatttagaa acactttacg
    22621 tagggggcgg gacaccaact tctttatctg ctaaacaact tgatcgactg ttatcaggtg
    22681 ctagagagat cctgcctttt caaaatggta atgagtttac tgtagaagcg aatccaggtg
    22741 atttgacccg tgaaaaactt caagtaatga aaaactatgg cgtcaatcgt ttatccatgg
    22801 gagtgcagac atttgataat cgcttattga aaaaaattgg acgtaaacac accgcagaag
    22861 atgtgtatca aactatgcaa tttttagaag cagaaaattt taccaatgta agcatcgatt
    22921 tgatttatgc attaccggga caaacactag aaggatatct ggatactttg gaaaaagctt
    22981 tggctttgga tctgcctcac tattctttgt actcattgat cctcgaaaac aaaacaatgt
    23041 tcatgaactg ggtacgacaa ggaagactcg aactgcctga tcaggaaacg gaaaccagaa
    23101 tgtttgaaga gacgattgaa gcaatggaaa agcatggacg tcaccaatat gaaataagta
    23161 actttgggct ctccggacat gaatctcaac ataacctcat gtactggaac aatgaccact
    23221 attttgggtt tggagctggt gctagcggtt atcttggaga tattcgttac cgcaacaaag
    23281 gaccaatcca acactattta cgcccattac gagcaaatga acgcccggta ttggaagaag
    23341 aaaaattgac acgaaaaaat caaatagaag aagaaatgtt cttaggacta agaaaaaaaa
    23401 cagggattac taaaaaacat ttctatgatc gctatcaaaa aacaattgaa tcggtgtacg
    23461 gtgatgtgct aagggaatta gagcaagaag ggttacttgt taatgaagaa gaccgtgttt
    23521 atttgacacc aagagggacc tttttgggga acaatgtgtt tgagcgcttt ttgtttgaaa
    23581 aatagtttaa agaatatttg ttttttatct gaatttaaat aaaaaaagtt caaaaataca
    23641 ataaaggact attgaaacgt tgatataact ggatttacat caattattta gaattagcac
    23701 tcttgtgttt agagtgctaa aaaatggagt ctttctcttg acaggagaag ggcttcttgc
    23761 tatatttata agtggattag cacttagaat atatgagtgc taatgagagg tgaggaagat
    23821 gttaacaaag cgacaaaacg acattttacg tctgatcatc caacactata cgagtagtgg
    23881 cgtgcccgtt ggttccaaaa cattgatgtc tgagggagtc aaggccagct cagcaacgat
    23941 tcgcaatgat atgaaagcat tagaagatga aggattgctg ttgaagactc actcttcttc
    24001 tgggcggatt ccatctgctt taggctatcg ttattacgtg gatcatttgt taaaacctgc
    24061 tcgcgtagca gaagatgatt tgctgctgat tcgtcagtct ctaggcagag aatttcatga
    24121 aatcaatgag attattaagc aatctgctga gatattatct gagctgacaa gctatacgac
    24181 tttttcattg ggacctgaaa taaaagaccg ccgactgact ggctttcgta tcgtaccctt
    24241 gaataatcga cagattattg ctatcgtcgt aacagatatg gggaatgtag aaagccaagt
    24301 gttcactctt cccgaatata tgggaagtca ggaactagaa aaaatggtac agattgtaaa
    24361 cgaccgtcta gtcggagatc ctctgatgac ggtgtaccat aaacttcgta cggagattcc
    24421 gatgattctt cataaatatt tccaaacgac agaagggatg tctcagttat ttgacaccgt
    24481 tcttggtcat gtttttgaag ataaggttta tgtcagtgga caaatgaatc tcttggatta
    24541 tgagccccgt caagatcttg atcagttcaa atcgatgttt tcttttatga aagactcaga
    24601 cggacttaca caaatgatca ctccgatgga tagcaatatt catatcaaga ttggtgctga
    24661 attaggcaat gatttattgc agaatatgag tatgattcaa gcaagttacg agatagatgg
    24721 tcatggaaga ggtacaatag ccttgttagg gcctactagt atgccttatt ctcggatgtt
    24781 cggtctagtc gatgtttatc gaagagaact agcagctaaa cttgctgatt actaccgttc
    24841 gcttgacttg tcaaactcgt aatgtgaata acgaagctat ttgcagctga tcgttctctc
    24901 tttcttagta agagcaatca aacagctgac tgaataatga aaggaagact gtatcgtgaa
    24961 agaaaatcaa gaagaactag ataaagaaat gacagatgct cagccagaac cggaaatcga
    25021 tgtcgaagct gcagaagatt cgggtatttc agaagctgaa gcggaagaat tcgaaacagc
    25081 gaaattaaaa gctgagttag aagaaatgga agataaatac ttgcgggctc gtgctgaaat
    25141 cgcaaatatg gcaaatcgag gaaaaaatga gcgtgagcaa ttacaaaaat accgttcaca
    25201 agatttagcc aaaaaattac ttccatcaat tgacaacctg gaacgtgcat tagccacaga
    25261 agtgtcagat gatcaagggg caggtttgaa aaaaggcgtg gaaatggtct tggaaagttt
    25321 gcgtaacgca ctggaagaag aaggaattga aaaaatccct gctaaaggag aagcctttga
    25381 tcctaatctt catcaagcag tacaaactgt tccagcgact gaagatacac cagccgatac
    25441 gattgtagaa gtactacaag aaggctacaa attacatgat cgcgtgttaa gaccaaccat
    25501 ggtcattgtt gcacaataat tgtgcggata taaaacttac actttcaatt aaagaaggag
    25561 acgttaatta tgagtaaaat tatcggtatt gacttaggta cgactaactc tgcagtagca
    25621 gtattggaag gaaacgaagc aaaaatcatc gctaacccag aaggaaaccg tacaacacct
    25681 tctgttgtat cattcaaaaa tggagaaatc caagtagggg aagttgcaaa acgtcaagct
    25741 gtgacaaacc caaatacgat ttcatcaatc aaacgccata tcggtgaagc aggttacaaa
    25801 gtagatgtag aaggtaaatc atatacacca caagaaatct cagcaatgat ccttcaatat
    25861 atcaaaggat tcgcagaaga ctatttaggt gaaaaagtcg aaaaagcagt tatcactgtg
    25921 ccagcttact ttaatgatgc acaacgtcaa gcaacaaaag atgctggtaa gatcgctggt
    25981 ctagaagtag aacgtatcgt caatgaacca actgcagctg ctcttgccta tggtttagat
    26041 aaaacagatc gcgacgaaaa aatcttagta ttcgaccttg gtggcggtac ttttgacgta
    26101 tctatccttg aactaggaga cggtgtcttt gatgtattgt caacagctgg cgacaaccat
    26161 ctaggtgggg acgactttga taacaaaatc atcgactaca tggtagcaga attcaaaaaa
    26221 gaaaacagca ttgacttgtc tcaagacaaa atggctttac aacgcttgaa agacgctgct
    26281 gaaaaagcga aaaaagattt atctggcgta tcaagcacac aaatcagctt gccatttatc
    26341 acagcaggag aatctggacc attgcatttg gaaatgacat tgacacgtgc aaaatttgat
    26401 gaattaacag ctgacttagt agaacgtaca aaagtaccag tacgtcaagc gctaaaagat
    26461 gcaggtttat cacaatcaga aatcgacgaa gttattttag tcggtggatc tactcgtatc
    26521 cctgcagttg tagaagcagt acgtaaagaa acaggaaaag aaccaaacaa atcagttaac
    26581 cctgatgaag tagttgctat gggtgctgct atccaaggtg gcgtgatcac tggtgatgtg
    26641 aaagatgttg ttttacttga cgttacacca ttgtcattag gtatcgaaac aatgggtgga
    26701 gtgttcacta aattgattga ccgcaataca acgattccaa caagtaaatc acaagtattc
    26761 tcaactgcgg cagataatca accagcagta gacatccatg tattacaagg tgaacgccca
    26821 atggctgctg acaacaaaac tctaggaaga ttccaattga cagatattcc agcagctcct
    26881 cgtggtattc cacaaatcga agtaacattc gatattgata aaaacgggat tgtaaatgta
    26941 tctgcgaaag atctaggtac acaaaaagaa caaaaaatca cgatcaaatc ttcttcaggt
    27001 ctaacagacg aagaaatcga acgtatggta aaagatgcag aagcaaatgc agaagcagat
    27061 aaagctcgta aagaggaagt tgacctacgt aatgatgtag atgcattgct attctctgtg
    27121 gacaaaacat tgaaagaatt agaaggcaaa gtggacgaag aagaagtgaa aaaagcagaa
    27181 acagctcgcg atgaattgaa agccgctgtt gaagcaaaca acatcgaaga aatgaaaact
    27241 aaacgcgatg cattgaacga aatcgttcaa aacttgactg ttaaactata cgaacaagcg
    27301 gcacaacaac aagctcaaga aaacccagaa gcagctcaag gcggagcaga tgatgtcgtt
    27361 gatgctgatt ttgaagaagt cgatggcgac gataaataat cagttcactg aatttcgaga
    27421 gaaggccaaa gttgctactt tggctttttc ttttgaaaaa tagtttgaag tatcggtttt
    27481 tgtttcaaac aaaatatgat atgataacaa gcgacgtgaa aatgatgaga accaatagga
    27541 ggaaagccta tggcaacaaa acgtgattat tatgaagtct tagggctctc aaaaggagca
    27601 tcagaagatg aaataaagaa agcttatcgt aagctttcga aaaaatacca tcctgacatc
    27661 aataaagaac ctgatgcgga agaaaaattc aaagaagtat cagaagcata tgagatttta
    27721 agtgacccgc aaaaacgcgc tgcatatgac caatacgggc atgcaggcac agatccaaat
    27781 tacggtgctg gtggagctgg tggcggattt ggtggattcg gcggcttctc tggtggcggc
    27841 ttcggtggtt ttgaagatat cttcgactct ttctttggag gaggcggtcg gtcagtcgat
    27901 ccaaatgcgc ctcgtcaagg tgctgatttg caatatacgg tcgacttgtc ttttgaagaa
    27961 gcgatttttg gtgtagaaaa agaaatcaag tataatcggg aagaaatctg tcatacttgt
    28021 ggtgggaacg gtgcaaaacc aggcacacaa ccaactactt gtcataaatg tcatggtgca
    28081 ggtacgatta atgtagaacg ccaaacacca cttggacgag tgatgagccg tcaaacttgt
    28141 gatgtttgtc atgggacagg gaaagaaatc aaagaacctt gtccaacctg tcatggctca
    28201 ggacatgaga agaaagcaca ttcagttaaa gtgaatgtac cagctggtgt agaagaagga
    28261 caacaaatgc gtttagctgg tcaaggagaa gctggagaaa acggcggacc atttggtgat
    28321 ttgtatgtgg tcttccgtgt agaagaaagt gatatctttg atcgtgaagg ttctgaaatt
    28381 tactatgaat tgcctttaag ttttgtacaa gcagctttgg gggatgaagt ccaagtacca
    28441 actgtccatg gagatgtgaa attgaagatc cctgctggaa cacagacagg tacgaacttc
    28501 cgtttacgtg gaaaaggcgc tccgaagtta cgtggtggtg caacaggcga ccaacatgtc
    28561 aaagtaaaac tgatcacacc gaaaaatcta aatgaacaac aaagagaagc attacgtgca
    28621 ttcgctgaag ctggcggcat caaagttgaa gaacagcaag aagacggttt cttcgataag
    28681 tttaaagatg cttttggcgg gaaaaagcgg aagtagtttt tctagtaaaa acgctaagtc
    28741 attaataatt aaaaaccaga cgaacaatca tatgttcgcc tggttttttt gtagttgtca
    28801 tgtttgtacc aacaaaaaag ccagctttag aatatgataa tcgtcatagt ttctaggggt
    28861 ataaccagtg agagaatgga acttgttcaa tcgatattgc aaggtgtttt tatgaataaa
    28921 taattcttct gagcaatttt tgatgcttcc gttattttta ctgaagcatg ccaaagtctt
    28981 cttgaacaaa tgtacttctt cttctgtcag gcttttcaaa atacgtttta catataatgt
    29041 tcgttgatct gacgggattg ctggtaaaac gatcccgagg tctaaatctt caaaaaagcg
    29101 gatctcgttc acagattgat ggaaagctat ccattcgcaa acttgtttgg catgaaggaa
    29161 agattgcggt gtctcttgct ctgtataaca aagcgaccca attccaaagt ttagctgtaa
    29221 ctgatacatt ttattaaact ttgtctggat tttatcaagc aatgatagca atgaggctct
    29281 ggaaatccga ttgactagga taatgatttg ttgtctatgt acagtgagca aagcctgtcc
    29341 gtaataagag aagacccttt cgagcaactc caataaagga ttgatccgtt cttgacacaa
    29401 ttgctgattg tcagaaagtt gtcccacaac tacataatat ggagaagaaa aatcaagctc
    29461 aattaagtga gggtggtatc gactatgtac ttgatatcat caatgaaccg atggtacttt
    29521 cttatgcgat ggaacaggta gagcgattgc ttgtttgtgc aggaaaacaa gcgatgcggc
    29581 tgatacgatt aggtgagaaa ataagaagat aaaaatacac ggtcccttca tggcgaccgt
    29641 gcatttttgg tttttattaa atatttaata ctttattcaa gaaatctttt gtccgttcat
    29701 tttgcggatt ttcaaagatt tgttgaggcg ttccatcttc taaaaagttt ccgtcgtcaa
    29761 tgaacatgac gcgattggcc acttctttag cgaaccccat ttcgtgcgta acgataacca
    29821 tcgtcatacc ttgttctgct aactttttca tgacgttcaa gacatcaccg accatttcag
    29881 gatcgagtgc agaagtaggc tcatcaaaaa gcatgatgtc agggttcatc gctagagcac
    29941 gagcaatagc cacacgctgt ttttgtccac cagaaagcat ttcaggatac atatcttttt
    30001 tatcttctag accaactgtc tgcagtaatt tgattgcttt tttctctgct tcttctctag
    30061 attctttctt caggtccatc ggtgctaaaa tgatgttttc caaaatagaa agatgaggga
    30121 ataggttaaa gtgttggaat accattccga tatgttgtct caccaagttg atatctgtgc
    30181 ttttatccat caagtgggcg ccgtcaataa taatttcacc gtcagaaggt tcttctaaac
    30241 gattcaagca acgcaagaat gtactttttc ctgaacctga agggccaatg atacagacga
    30301 catctccttc gttgatggat acattgatgt cgtttaatac tgtgttatcg ccgtattttt
    30361 tgactaaatg ctcaactaag attttttctg ccatcttact tcaccttctt ttctaaaact
    30421 tttgctaatt ttgtcaatgc tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa
    30481 acatacgtac tttgtgttgt acgtgcaaca atgatcttac cagtttgcag caattcgact
    30541 acaccgattg cggaaatgat cgttgtgtct tttaatgaga taacgaactg attgacgaat
    30601 gatgggatca tgatgcgtat cgcttgcggc aaaatgattt tttgcattgt ccgattataa
    30661 gaaagaccta aacttctgga agcttccatt tgtccgacag gaacggcgtt gatcccgcct
    30721 cgaacaatct ctgcgatata agcacttgcg ttaagagtca aggtgataac accagcaagg
    30781 aagtcaggaa tcgtaaatcc aacgatacca ggcaacccaa agaaaataaa gaacgcaaga
    30841 accatcattg gaattcctcg gatgatatcg acataaatac tagcaattgt gcgaagagcg
    30901 cgtattggtg ctacactgaa taatccaaaa atcacaccga ttactatagc caaagcaaag
    30961 gagatcaaag ttaggagaat tgttctccaa agtcctttca acaatgcttg atagttgttt
    31021 tggataagtc caagaaatgt tgattcatca gcagtggaag actcactgga agtttctgaa
    31081 ccatccgcta catattcttt aataatctta tcgtattcgc ctgttctttt taattctttc
    31141 aatccttcat tgaacatatt aagaagctca ggattttgtc cttttttaac ggcaaatgca
    31201 tattttccgc cagattcttt tttgatcggt gtttccaatt cttgtccttg tttgatcgca
    31261 taaccgatta ccggataatc atccatcatt gcatcgattg cgccaacttt taacccgtcg
    31321 tacaattgat cagcagcatc aaaatatttg atagaatagc catattcaga ttcatggtcg
    31381 cttaaaaatt cagcactttc ggtaccaact tttgctccga ctgtttttcc tttcaaatct
    31441 tcataagact tgatcgaagt atctcctttt ttgacagcta attggatccc gctatcaaag
    31501 taagaatcag aaaaatcata gcttttcttt cgttcatctg ttacagtcat tccggcaatc
    31561 attccgtctg cttgtcctga ttccaaagct tgcaaagcag cagagaaacc acggtagtcc
    31621 attcggatgt taaatccttg cagtttggcg atgttttcca tcaattttac atcgatgcct
    31681 tcgtactcgc cctttgcgtt ttgaaattca aatggagcaa aagcagagtc actggcaatt
    31741 acgtatacgt cttttttggg ctctattttt tgcattgtct cgcttgtgct tgactctgcc
    31801 gttgtagtgt tgtcagcttg tgcaacatga ccaaacgcaa atgtcacaag tcctgtcatt
    31861 aagaaaacaa atgagtgaaa taaatgcttt cttttcataa tcaacctcct aaatttccaa
    31921 tattttatta ttataggcga aaagtgctga atcagacaag actgtaagct cgaaaaaatg
    31981 aaaaaaatgc gagtttctaa cattttatgt tataaaacat aacatgtata ataagataaa
    32041 aaagtggcga aagtatgttc ggtgtggtat aattttttag taaggaatgc ctttgctttc
    32101 cttttctttt tattggtcgt aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga
    32161 ttgagagaga tacctcccga cactttgagc ttgtctcgaa ataccaaccg gctggtgatc
    32221 agcctgaagc gattaaccaa ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct
    32281 tattaggtgc gactggaaca gggaaaacat atacgatttc caatcttatt gagaaagtga
    32341 ataaaccaac actgattatt gcccataaca aaacattggc tggtcagctg tacggggaat
    32401 tcaaggaatt tttccctaac aatgctgttg aatattttgt ttcttattat gattattatc
    32461 agcctgaagc ttatgtgcct tcaagcgata cgtatatcga aaaagattca agtgtaaatg
    32521 atgagatcga caaattacga cattcggcta ctagttcatt attagaaaga aatgatgtga
    32581 tcgtcattgc atccgtttca tgtatttttg gattaggctc gccttttgag taccaaaaac
    32641 aagtagtttc gattcgacag ggagcagaac tagatcgcaa tcagttgatt cgtgacttag
    32701 tcagtatcca attcgaaaga aatgatatag attttcagcg cggtcgtttt cgagtgcgcg
    32761 gtgatgtcgt ggagattttt ccggcttcaa gagatgaacg tgccttacgt gtggagtttt
    32821 ttggagatga ggtcgaacgt atccgagaag tcaatgcact gacaggtgaa gttttaggag
    32881 aaacggaaca cgtagcaatc ttcccggcga ctcactttgt gacgaatgat gaacatatgg
    32941 agcatgccgt agcgaatatc aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg
    33001 aaaacaaact tctggaagct caacgtttag aacagcgaac caattacgat attgaaatga
    33061 tgttagaaat gggctacacg tcgggaattg aaaattattc tcggcatatg gacggacgta
    33121 aagaaggtga accgccttat acgctattgg atttctttcc tgaagatttt ttgatcgtgg
    33181 cggacgaatc gcacgtaacc atgcctcaaa tcagaggaat gtataatggt gaccgagcga
    33241 gaaaacagat gctagtagat tacggtttcc gtttgccatc tgctttagac aatcggccac
    33301 ttcggttaga agagtttgaa aaacatgtca atcagatcat ttatgtctct gcgacgccag
    33361 gaccatatga acatgaacaa acagataccg ttatccaaca aatcatccgt ccgacagggc
    33421 tgcttgaccc agtgatcgaa gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg
    33481 aaataaatga acgagtcgaa aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa
    33541 tggcagaaga cctcacagat tatttcaaag aattagggat caaagtcaaa tatttgcata
    33601 gtgatatcaa aacgctggaa cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg
    33661 atgtgttagt tggaatcaat ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg
    33721 ttgctatctt ggatgcggat aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa
    33781 cgattggacg agctgctcgg aatgaagaag gtaaagtaat catgtacgca gacaaagtaa
    33841 cagattctat gcgtctagcg atggatgaaa cgtcaagacg tcgtacgatt cagcagaagt
    33901 acaatgaaga acatgggatc gttccaaaaa cgatcattaa agaaatccga gatttgattt
    33961 ctattacgaa agaatcggaa gatgatacaa aagaagcggt acaagtctcg tacgaagaaa
    34021 tgacgaagga agaaaaagac acgttattga tgaaattaga aaaagaaatg aaagatgcgg
    34081 caaaagcttt ggattttgaa acagccgcga atgtgcgaga tatgatttta gaattaaaag
    34141 cttcaaagta ggaggacaac atggctaacg ataaaattac gattcatggt gcacgtgccc
    34201 ataacttgaa aaatatagat gtaacgattc cacgagataa attcgtggtc gttacaggtt
    34261 tgtccggttc tggaaaaagt tccctagcat ttgatacgct gtacgctgaa gggcagcgga
    34321 gatacgtaga gagcctttct gcttatgctc gtcagttttt aggacaaatg gataagccgg
    34381 acgtagatag catcgacgga ttgagtccgg cgatttctat tgatcagaaa acaacaagta
    34441 aaaatcctcg gtcgacagta ggtactgtga cggaaatcaa tgactattta cgcttgttgt
    34501 ttgcacgtgt aggacatccg atttgtccca acgatcatat cgagattacc agtcaatcag
    34561 tagatcaaat ggcagatcaa gtaatggctt tgcctgagcg cacaaagatc caagtgttgg
    34621 caccgatcgt ggtaaagaaa aaaggacaac ataaaaaaat attcgaacga atccaaaaag
    34681 agggatacgt ccgcgttcga gtcgatggtg agatctatga tttaagtgaa gtgcctgagc
    34741 tggagaaaaa caaaaaacat gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag
    34801 ggattcgttc tcgcctattt gattctttag aggcggccct gcgattagca gacggctatg
    34861 ctctggttga cgtgatcggt gaagaagaaa tgttgtttag cgaacactat gcctgtcctt
    34921 attgcggatt tactgttggc gaactagaac caagactgtt ctcattcaat gcgccttttg
    34981 gagcttgtcc agactgtgat ggtttaggtg tcaaattaga agtcgatcaa gatttagtga
    35041 ttccagatag aacgaagaca cttagtcaag gtgctatcgt tccatggaat ccaatcagct
    35101 ctcagtatta tcctcaaatg ttagcgcagg cttgtgaaag tttcggtatc gatttagata
    35161 caccattcga agaattaccg gaagagcatc aggagatcat cttgaatggg acagatgatc
    35221 ttttccattt ccattatgaa aatgattttg gtggcgttcg cgatgtagaa acgactttcg
    35281 aaggggtatt gaagaatatc gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg
    35341 agcaaatgcg tctttatatg acagaattaa cttgtcagac atgtaaaggt tatcgattga
    35401 atccccaagc tctatctgtt caaatcaatg gaacgaatat cgggcaaaca aatgagttgt
    35461 ctattcaaaa agcagtagat ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa
    35521 ttgccaaacc gattttaaaa gaagtaaatg acagactttc attcttggaa aatgtgggac
    35581 tagactatct aactttgagt cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa
    35641 tcagattggc tacacaaatt ggatcgaatc tttctggagt tttgtatatt ttagatgaac
    35701 catcgatcgg attgcatcaa cgcgacaacg atcgactgat tggctcatta aagaaaatgc
    35761 gtgatcttgg caacaccctc gttgtagtcg aacatgatga agatacgatg tgggcagcag
    35821 attatttgat tgacgtcgga cctggggccg gagaacaagg cggacaaatc gtggctgcag
    35881 gcactcctga agaagtagct aatgatgaaa attctttgac agggaaatat ctttcaggga
    35941 aaaaatcaat ccctgtacca aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca
    36001 ctggtgcttc agaaaacaat ctgaaaaata ttagtgttga atttccatta ggagaatttg
    36061 tagcagtcac aggtgtgtct ggttctggaa agagtacatt ggtcaatagt atcttaaaga
    36121 aatcgttagc gcaaaaatta aataagaatt ctgctaagcc aggtaaattc aagacaattt
    36181 ccggctacga aagtatcgaa aagatcatcg atatcgatca aagcccaatc ggccggacgc
    36241 cgagaagtaa tccagcgact tatacaagtg tatttgatga tatccgtggg ttatttgctc
    36301 aaacgaacga ggcaaaaatg cggggttata agaaagggcg ttttagtttc aacgtaaaag
    36361 gcggtcgttg tgaagcttgt cgcggggatg gaattattaa gatcgaaatg cactttttgc
    36421 ctgatgtcta tgttccttgt gaagtatgtc atggtaaacg atataattct gaaacattag
    36481 aagtgcatta caaaggaaaa agcattgctg atattttgga aatgacagta gaggatgctg
    36541 tagaattctt caagcacatt ccaaagattc atcgcaaact gcaaacgatt gttgatgttg
    36601 gcttaggtta tgtgacgatg gggcaaccag caacgacatt gtccggtggt gaggcacaac
    36661 ggatgaaact tgccagtgaa ttgcacaaaa tctctaatgg aaagaatttc tatatactag
    36721 atgaaccaac gacaggactt catagcgatg acatcgcccg cttgttgcat gtattacaaa
    36781 gattagtaga tgctggtaac acagttttag tgattgaaca caatctagat gtaatcaaaa
    36841 cagcagatta tatcattgat ttaggaccag aaggtggaga aggtggagga acgatccttg
    36901 cgactggaac accagaagaa atcattaacg taaaagaaag ttatacaggt cactatttga
    36961 aaaaaataat ggtataaaga ggatagatag ctgagatagt taaaaaaaca taaaaaaatg
    37021 aatttttgct tgttttatta atatcagtgt tctagaatca ttacatggta gttctttcta
    37081 cactgattgg tatagataaa aacttcctat aagttcaatc cgttcaaaaa agaattgagc
    37141 aatcggagga ctttcctttg attgcttttt tctttatggt atgatattcc ttgtgtagtt
    37201 ggaaaagaaa aacaagagat tatgagtaag tgagagataa tgatgaacag tattgaaaat
    37261 gaagaattta tccctatatt attaggaagc gacatgaacg tttatggtat ggcacgttct
    37321 tttaatgaag catacggcaa aatctgccaa gcatatgctt cggatcagtt agcccctacc
    37381 cgctacagta aaattgtcaa tgtagaagtt attccaggat ttgataaaga ccctgtattt
    37441 atcgagacga tgcttcgttt ggcaaaagaa agatattctg ataagagtaa aaaatatctt
    37501 ttgattgctt gtggtgatgg atatgcagaa ttgatctccc aacataaaca agagctgtca
    37561 gaatatttta tctgtccata tattgattat tctttatttg aaaggctgat caataaagta
    37621 agcttttatg aagtttgtga agaatatgat cttccgtatc ctaaaacatt gatcgtcaga
    37681 gaagaaatgt tagtaaatgg acatttggaa caggaattgc cttttgaatt cccagttgct
    37741 ttgaaacctg caaacagtgt ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct
    37801 tttattttag agactagaga agagttcgat ttaattttag gccggatata cgaagccggt
    37861 tataaaagtg aaatgattgt acaagatttt atcccgggtg atgatagtaa tatgcgtgtg
    37921 ctaaatgcgt atgtagatga agatcatcaa gttcggatga tgtgccttgg tcatccacta
    37981 ttagaagatc ctacaccagc atcgattggt aattacgttg tgatcatgcc agattataac
    38041 gaaaaaatct atcaaaccat taaggcattt ttggaaaaaa tcgaatatac aggctttgcc
    38101 aatttcgata tgaagtatga tcctagagat ggagaatata aattatttga aatcaatttg
    38161 agacaaggtc gtagcagttt ctttgtgaca ttgaacggct tgaatctggc tcgttttgtc
    38221 acagaagatc gagtgttcaa caaacccttt gttgaaacga cctatggaac gaatcaatca
    38281 gataaagcaa gattatggat gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa
    38341 aatgaagaca aaaaactagc tgagcaaatg atcaaagaaa atcgttacgg tactactgta
    38401 ttttatgaaa aagaccgatc aatcaagcgc tggttgttga tgaaatatat gttccataac
    38461 tatattccaa gattcaaaaa gtattttcat gtgaaagaag gctagttcat ggagaatttt
    38521 ttcagtattt taggcggaat gggcacgatg gcgacagaaa gttttgttcg cttgatcaac
    38581 caccgaacga aagctacaaa agatcaagaa tatttgaatt atgtcttatt caatcatgca
    38641 acagttcctg atcgtacagc ttatatttta gatcgatcag aagaaaatcc aatgccgttt
    38701 ctgctggatg atattgagaa acaaaattta ttgcgaccaa acttcattgt gttgacttgt
    38761 aacaccgcac actacttttt tgaagagtta caagcggcaa cggatattcc tatcttgcat
    38821 atgccaagag aagcagcaaa tgaacttgtc cgtcagcata cgacagggag agtagcgatt
    38881 ctaggaacag aaggaagtat gaaagctgga atctatgaac gcgaagtcaa aaatcttggt
    38941 ttcgaaacag tgattcctga tactgcgctt caagaaaaaa tcaattacct gatctatcat
    39001 gagatcaagg aatcggatta tttgaatcaa gagctctatt acgaaatctt agaggaagcg
    39061 gtagagcgcc taaactgtga aaaagtcatc ctaggttgca cagaattatc tttgatgcat
    39121 gaatttgctg aagataatca ttacccagtg atcgatgctc aatctatttt agcggatcga
    39181 acgatcgaac gtgcattagc tgaaagaagc gaggcattgg atacagcatc ggaaaagtaa
    39241 aaaaattgga tttggggcaa atatcctaaa agatatttgg tccaaatctt tttgtaaaag
    39301 aaaaagaaaa ctggtaatga tacgcccggg atgatataat tttattatgc acaatgtgag
    39361 aggggttcgt aaatatgaca gataatttgc aattagtcat cataacaggt atgagtggtg
    39421 cagggaaaac agtagctatc caaagttttg aagatatggg ctatttttgt atcgataata
    39481 tgccgcctcg attgattcca aaattctggg aattgatcaa agaatcaggt aaagtcacaa
    39541 aaatggcttt aggagtggat ttgcgttctc gttccttctt tgaagaaatc caaaacatgc
    39601 tgatcgaaat cgaaaatacg aacttcatcg acacttctat cttattttta gatgcttcag
    39661 acgaagaact agtggctcgc tacaaagaaa caagacgagc acatccaatg gcgatggacg
    39721 gtttgatcac tgaagggata cgtaaagaac gggcgatact agacgacttg aaagcccaag
    39781 cttccgtgat cattgatacg acaacattga caccacgaca attacgtgaa aaaatcaatg
    39841 agtcattcaa atcaaaggga gaccaaggat ttcggatcga atttgtatca tttggtttta
    39901 aatacgggtt gccgatcgat tcggatattg tgatggatgt gcgttttttg cccaaccctc
    39961 attatatccc ggaactaaga ccattgaccg gaaaagatca gccagtctat gattatgtga
    40021 tgtcctttga tgaaacgcaa tcattctacc ataaattttt ggatcttctg gaaactgtat
    40081 tgcctggata cattaaagaa ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg
    40141 gtcaacaccg ttctgtggca ttgactgaac gtttggcaaa tgctttaggg aaaaactata
    40201 aagtcaatgt gacacatcgg gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa
    40261 aatgaaaacc taccggataa gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct
    40321 gccagttatc ctgaaaagtt tacgaaatca aggagcagat atcacagcag tagtgactgt
    40381 tgcagatgat ggtggaagca gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg
    40441 tgacttgaga aatgtcttag tggcgttatc ggatatgcca caattctatg aagatatttt
    40501 ccaataccgt ttcaaaaagg aagatcagtt tttagccaat catacgatcg gtaatctgat
    40561 cattgccgca gtttccgaga tgcggggaag cacttatgag gcgattcaat tattagcaaa
    40621 aatgatgcat gtagatggac atgtttatcc ttcttcagaa actcctttga ccttgcatgc
    40681 tgtttttaaa gacggaacga ctgcagtagg agaatctaaa atcgcgatca accgtaaaac
    40741 cattgaccgt gtgtatgtct gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa
    40801 agtcgtttca gcaattatgg acgccgacat ggtcgttttg ggacccggaa gtttattcac
    40861 cagtatcctt ccaaatttag tgataccaga aatcggcgaa gcaatgctca agacaactgc
    40921 tgaaaccgtc tatatctgta atatcatgac ccaaaaaggg gaaacggaac atttcacaga
    40981 tgccgatcat atacgtgttt tacacaagca tttgaatcag ccttttatcg atacagtatt
    41041 ggtcaatacg gaaaaagtgc ctgaagatta tatggaccca gagatctatg atgagtatct
    41101 agtacaagtc gcacacgact tcaacggatt gagaaatgaa ggatgtcgag tgatttccac
    41161 cgacttttta gaattgaaag atggtggtgt cttccacgat ggagacaaag tagtagaaga
    41221 attgttccgt ttagttttcg gttcaaaata ttaagcaatt tattgttgga gaaaggagga
    41281 gaataggatg tcatttgctg cagatgtgaa gaaagaacta actggtttgg aagttcatcg
    41341 agaacatgcg aaagcggaat tagctgcttt gctgcgaatg aacggctctt taggtattgt
    41401 gaaccagcag tttattttga acgtccaaac agaaaatgct gcaattgcca gacgtatcta
    41461 ttctcttttg aaagatcatt atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa
    41521 attaaaaaag aacaatgtct atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat
    41581 ggatcttgat atcatggatg gtatgatgtt caatggacac gtttcggacg agattatggg
    41641 aaattcgcaa aaaatgcgtt cttacttacg tggtgctttt atggcctcag gttcagtcaa
    41701 taatccggaa acgagtcgtt atcatttgga aatattttct atttatgaac agcataacca
    41761 agacatttgc gatatgttaa attattacgg tctacatgct cgaacgcttg aacggcgcaa
    41821 tggctatatt tcgtatttaa aaggagcaga aaaaattgct gatttcttaa cattgattgg
    41881 tgcaaccaac tcaatgctcc gatttgaaga tgttcgaatc gtacgggata tgagaaattc
    41941 ggtcaatcgt ctagtcaatt gtgagacagc aaatctgaac aagacgattg atgcggcttc
    42001 taaacaaatc gaaaacatcg aattgatcga agcaaaggtc ggtctacatg cccttcctga
    42061 aaaacttcag gaaatcgcag aacttcggct tcaacatcca gaggtcagct tgaaagaatt
    42121 aggggaaatg attccctcag gtgcaatatc aaaatcggga attaatcacc gaattcgcaa
    42181 aataaatgaa tttgcggaaa aactgcgtga agaagcagtt taatccaaag aaaaaactga
    42241 tacatgaagc aggagatgcg aatgtatcag ttttttttaa aagctatact tgcttttctt
    42301 ccgtcaaact ataattccaa ctttactgga gtaacataat gttgaaaggc ttctattgtt
    42361 ttttaataca atcgtataaa aagcggggag acaactgaat gaagatttta gttttataca
    42421 taaacttttg cttaatatag aagtcctgat atagatagga tcaagtattt atctataact
    42481 ggagattaat taaaaactgg aaggaactgc tcgatcaacc ttgagaactg tttttcttac
    42541 ttctggtcaa gattctcgtg gttggtggat aaggatggtt gataagttca ttggattagc
    42601 tagtcgaaaa caaccaggtg taattaatgt agaagtttta acaaggggat ggagtgattt
    42661 attcagtgat aatcataagt tcataaatag taattgggat gtatggttgg tatacgttat
    42721 acctgaactt gaactatcaa gatttggttt agaatttgag aatcgatgtt tataaaggga
    42781 gggcaaaaaa tgtgcttttt tacaactttt actatcatta tgattgcagt actttttatt
    42841 tttctagata tagcaaagcg aaatacagca tttttattat atcgggtttt acttcgagca
    42901 ggtctcataa catttatatc gatcgttgga ttttttctat tcacagttat tgtatttatt
    42961 tggagaacta cagcaccacc attgccagag attacatatg gagaatttcc gtttagtctt
    43021 gaatatgaaa tagatgagga aagaattttg attgaagata cggtgattgc agaatttata
    43081 ggaagtttta gagggaatgt gacaactcaa cctagacgaa gttggagtat aaaactttta
    43141 aataatagtg gagagttgaa agcatttcat tatgaattct taataataga aagtgaagat
    43201 gtaacaatca ctttaagtag tggctctgct ggatatttta tgaatgatgg tggaagcaag
    43261 ccacagctca cagtccgtga tcgtattgca ggttttatgc atttttctca ctatgactta
    43321 gaagaagctc gtaaaatact tgcagaatat gggatcacat taatcaattg ggaatatgcg
    43381 ccaccgataa gaaatttttt caatgattat tattagtctt ataaaaaaca gaatgtaaaa
    43441 tttatgtgcg cgagtattta gttttccatt ttttgtttga tagatatata gcattgttga
    43501 agcatgggat agtggtgcca attttatcaa tgaggttggt caggcagtta acataattgg
    43561 tgatgctata aatccattta attggaattg gaggaattag gtgtggaaac agtgagatta
    43621 tggatattca tgaaaaattt tcgttcggct ttgagatatg ttattctgga atcaaaatca
    43681 ggttattatc tgattgacac agatcactat actttttggg gaggctacct ttttccgatt
    43741 tttaattgga taatccccca taaggttatg aaaattaact tgcctgcaga agaaataaat
    43801 agattaatac tcaacaaaga tttacaaagg aaaagagaga aagaacattc taatgttgct
    43861 tggataggtc cagcatttgc agtagtgact tcttcaattg tcggtcctat gctagagagt
    43921 atttatagtg atttttctat tggaatacgt tatttatttg ttactttaag tatagctgtt
    43981 cttttgtgca taaagggtta tgttagcaag aagaataaag atattttaac actaatcggt
    44041 gtaaacaatt tatctagtac taaaattata ctgtttcctg aatctataaa agaaggatc
//
LOCUS       DAZUKR010000007        43809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000007 DAZUKR010000000
VERSION     DAZUKR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.007"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..458)
                     /locus_tag="ACG89C_000381"
     CDS             complement(114..458)
                     /locus_tag="ACG89C_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4898964.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(451..885)
                     /locus_tag="ACG89C_000382"
     CDS             complement(451..885)
                     /locus_tag="ACG89C_000382"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS4898965.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1023..1760
                     /locus_tag="ACG89C_000383"
     CDS             1023..1760
                     /locus_tag="ACG89C_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898966.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1753..2964
                     /locus_tag="ACG89C_000384"
     CDS             1753..2964
                     /locus_tag="ACG89C_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898967.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3168..3950
                     /locus_tag="ACG89C_000385"
     CDS             3168..3950
                     /locus_tag="ACG89C_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS4898968.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4020..4682
                     /gene="trmB"
                     /locus_tag="ACG89C_000386"
     CDS             4020..4682
                     /gene="trmB"
                     /locus_tag="ACG89C_000386"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4898969.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4780..6534)
                     /gene="ade"
                     /locus_tag="ACG89C_000387"
     CDS             complement(4780..6534)
                     /gene="ade"
                     /locus_tag="ACG89C_000387"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4898970.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6666..7595
                     /gene="comGA"
                     /locus_tag="ACG89C_000388"
     CDS             6666..7595
                     /gene="comGA"
                     /locus_tag="ACG89C_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS4898971.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7552..8601
                     /gene="comGB"
                     /locus_tag="ACG89C_000389"
     CDS             7552..8601
                     /gene="comGB"
                     /locus_tag="ACG89C_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS4898972.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8598..8876
                     /gene="comGC"
                     /locus_tag="ACG89C_000390"
     CDS             8598..8876
                     /gene="comGC"
                     /locus_tag="ACG89C_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS4898973.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8851..9321
                     /gene="comGD"
                     /locus_tag="ACG89C_000391"
     CDS             8851..9321
                     /gene="comGD"
                     /locus_tag="ACG89C_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS4898974.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9293..9640
                     /locus_tag="ACG89C_000392"
     CDS             9293..9640
                     /locus_tag="ACG89C_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898975.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            9603..10028
                     /gene="comGF"
                     /locus_tag="ACG89C_000393"
     CDS             9603..10028
                     /gene="comGF"
                     /locus_tag="ACG89C_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS4898976.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            10000..10359
                     /gene="comGG"
                     /locus_tag="ACG89C_000394"
     CDS             10000..10359
                     /gene="comGG"
                     /locus_tag="ACG89C_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS4898977.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10531..11538
                     /locus_tag="ACG89C_000395"
     CDS             10531..11538
                     /locus_tag="ACG89C_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4898978.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11563..12747
                     /locus_tag="ACG89C_000396"
     CDS             11563..12747
                     /locus_tag="ACG89C_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS4898979.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13184..14767)
                     /gene="abc-f"
                     /locus_tag="ACG89C_000397"
     CDS             complement(13184..14767)
                     /gene="abc-f"
                     /locus_tag="ACG89C_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS4898980.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(14872..15000)
                     /locus_tag="ACG89C_000398"
     CDS             complement(14872..15000)
                     /locus_tag="ACG89C_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898981.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(15345..15488)
                     /locus_tag="ACG89C_000399"
     CDS             complement(15345..15488)
                     /locus_tag="ACG89C_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4898982.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15710..16189)
                     /locus_tag="ACG89C_000400"
     CDS             complement(15710..16189)
                     /locus_tag="ACG89C_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4898983.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16262..16546)
                     /locus_tag="ACG89C_000401"
     CDS             complement(16262..16546)
                     /locus_tag="ACG89C_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898984.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16624..16818)
                     /gene="ssrS"
                     /locus_tag="ACG89C_000402"
     ncRNA           complement(16624..16818)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG89C_000402"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16890..18176)
                     /locus_tag="ACG89C_000403"
     CDS             complement(16890..18176)
                     /locus_tag="ACG89C_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS4898985.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18314..18985
                     /locus_tag="ACG89C_000404"
     CDS             18314..18985
                     /locus_tag="ACG89C_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS4898986.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18951..19439
                     /locus_tag="ACG89C_000405"
     CDS             18951..19439
                     /locus_tag="ACG89C_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS4898987.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19452..20399
                     /gene="prmA"
                     /locus_tag="ACG89C_000406"
     CDS             19452..20399
                     /gene="prmA"
                     /locus_tag="ACG89C_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4898988.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20400..21164
                     /locus_tag="ACG89C_000407"
     CDS             20400..21164
                     /locus_tag="ACG89C_000407"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4898989.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21397..23610
                     /locus_tag="ACG89C_000408"
     CDS             21397..23610
                     /locus_tag="ACG89C_000408"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4898990.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23621..24067
                     /gene="dtd"
                     /locus_tag="ACG89C_000409"
     CDS             23621..24067
                     /gene="dtd"
                     /locus_tag="ACG89C_000409"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4898991.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24238..25497
                     /locus_tag="ACG89C_000410"
     CDS             24238..25497
                     /locus_tag="ACG89C_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS4898992.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25586..26533)
                     /locus_tag="ACG89C_000411"
     CDS             complement(25586..26533)
                     /locus_tag="ACG89C_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4898993.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26927..28219
                     /gene="hisS"
                     /locus_tag="ACG89C_000412"
     CDS             26927..28219
                     /gene="hisS"
                     /locus_tag="ACG89C_000412"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4898994.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28234..30003
                     /gene="aspS"
                     /locus_tag="ACG89C_000413"
     CDS             28234..30003
                     /gene="aspS"
                     /locus_tag="ACG89C_000413"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4898995.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30112..30987
                     /locus_tag="ACG89C_000414"
     CDS             30112..30987
                     /locus_tag="ACG89C_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4898996.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31253..33136
                     /locus_tag="ACG89C_000415"
     CDS             31253..33136
                     /locus_tag="ACG89C_000415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4898997.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33211..33495)
                     /locus_tag="ACG89C_000416"
     CDS             complement(33211..33495)
                     /locus_tag="ACG89C_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4898998.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33485..34219)
                     /locus_tag="ACG89C_000417"
     CDS             complement(33485..34219)
                     /locus_tag="ACG89C_000417"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS4898999.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34348..34971
                     /locus_tag="ACG89C_000418"
     CDS             34348..34971
                     /locus_tag="ACG89C_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4899000.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35163..36284
                     /locus_tag="ACG89C_000419"
     CDS             35163..36284
                     /locus_tag="ACG89C_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4899001.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36291..39419
                     /locus_tag="ACG89C_000420"
     CDS             36291..39419
                     /locus_tag="ACG89C_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFS4899002.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39438..42632
                     /locus_tag="ACG89C_000421"
     CDS             39438..42632
                     /locus_tag="ACG89C_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS4899003.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42778..43485)
                     /locus_tag="ACG89C_000422"
     CDS             complement(42778..43485)
                     /locus_tag="ACG89C_000422"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4899004.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14140 a         7772 c         9239 g        12658 t
ORIGIN      
        1 taaaaacgtt gtcgtggtag tttcaaacaa aattcaaaat ttcatcacat tcctaggaaa
       61 tacgcaaact tttctcactt atatagttaa atctgttgag aaaataatca ttattatatt
      121 tgttcaggat actctggcag atcagctttt agtttttcta cttgttcatt gatttgtgtg
      181 atacgtggtt cggcttggaa tttaaaatct tcgatatctt ttttgattgc ttcttggaaa
      241 gcaggaatca cagattcagc tgtttctttt gtttcgatca atgcgctttt aaaatgattc
      301 aggctgttat ttaaatcatt tgtcagagtt gttgcttctt ccaattcttc gatcagtttc
      361 tttcttgttt cattcccgct cctgggcgcc aataacagac ctccgacagt gccagcagca
      421 gctccaaata acagtccttt gaaaaagttt ttaatcatcg caggacacct gctttacgat
      481 tgtttcttga atagctttta gttcttccga tccataacta tcttgatgat tgacaaagtg
      541 aatagaaaaa tcatcctctt tgctgtagcg agggactaaa tggatatgtg aatgaaaaac
      601 agattgatag gctagttcac ggttgttatt caagatatta agacctttca tttctggaaa
      661 agctttttcc aatgcttgag caatcttagg cacacgcgaa aaaacctctc ctgcaagttc
      721 gggatcatat tcaaaaatat ctgctacatg acgtttaggc acaactaatg tgtgtccttt
      781 cgttgcttga gagatgtcta aaaaagcata gaccttgtcg tcttcatata ttttatagct
      841 tgggatttct tgattgatga ttttgcaaaa aatacagttt tccatataaa acatcctttc
      901 tataaagaat aaatacttca gcttaacttt accataagtt aaagctattg taaggaaaaa
      961 aacaaaatac cgtcattttc ttcgtggtat aataaataaa aataaatggc ggaggaaaaa
     1021 gaatgagttt aattatcgat catgtcacag gggggtacgg acatctccct gtgttaaaag
     1081 atatcagttt cgatgtacag cctggagaga tagtagggtt gattggactg aacggagcag
     1141 ggaaaagtac gaccatcaag cacattatcg gtctgctgga agcttcaaaa ggaacgatag
     1201 aaatcaatca tctatcctta aagaaaaata cggaagaata tcgcaaacaa attgggtata
     1261 ttccagagac tccttcttta tatgaagaat tgacattgag agaacatatc gaagtcactg
     1321 cgatggccta cgatatacct atggatatag caatgaaaag agcagaaaag ttgttggaga
     1381 cttttcggtt atctaatcgt cttgactggt tcccagctaa tttttcaaag ggaatgaaac
     1441 agaaagtgat gatcttatgc gcctttttga tcgagccttc tttatatatc atcgacgaac
     1501 catttttagg cttggatcca ctagctatca atgcattgct tgaattgatg gtagaaatga
     1561 aaaaagaagg agccggtatt ttgatgtcta cgcatatttt agctactgca gaaaaatatt
     1621 gtgatcgttt tgtggtctta cataacggag agatacgagc agacggttct cttgagatgt
     1681 tgagaaaaga atttcagctg ccggattctt ctttggacga aatctatatt gctttaacaa
     1741 aggagcaggc acatgagtag tttttttcaa aaacgtttag caagacatca aaagaaaatg
     1801 atgaaatata tgcgttatgt attgaacgat cattttgttt tagtttgtct tttcttgatt
     1861 ggcggtgtgg gattatatta ttctaattgg ttgaaaaccc tgcagccacc gtttgcaatt
     1921 ggagggctca taatctctgt attttggatg ctctgtttat tcgtgggtaa gatggctact
     1981 tttgcagagg cagcagatat tgttttttta cttcctaaag aaaaagaaat gcaaacctat
     2041 ttggaacgag cttttcgcta ttcttgcatt tttccgttga tctttttgat attggcatgc
     2101 ggattggcca tgccattagt ggtggttagt acggagcaat ccttttcgat gtttttcgtc
     2161 tatctgatca tattatggag tttgaaattc agtcatctgc aaatcaagag agcagaattg
     2221 tttcgactag atcaacaagt tattcgttat tggaaattag gctggttttt ttcttctctg
     2281 atctttttgt ttctatcatt ttattggcta atatgggcag gaatagcagg tgcattagta
     2341 caggcaagtc tatattactg gtttttgtgg aagcaacaag aaaaaggatt ggattgggag
     2401 aaaatggtgc atgtagaaga acataggttg catcagatct atcaatttat caatttattc
     2461 acagatgttc ctgaaattac ggcaaagatc aaaagaagac gctatttgga tcctatattg
     2521 actcatatta aaagagaatc aaaaaacact tatctttact tgtttgcaag aagattggca
     2581 agaggttctg attttagtgg tctttatttc cgattagtaa ttataggtgg gttattgatt
     2641 gccggagtag tcgactttta ttttagtctg attatcggtt tcttattttt atatttgatc
     2701 ggtttccaac tgttaccttt agcgaaccag ttccgctata tggttctcgt tcagctttat
     2761 ccaatccata agaatcagaa gaagcaggcg attcagtatc tactcacatg ggtattaatt
     2821 atcgcgtcgg ttatttttgg tttgattgct gcagtcgtat tagatgggca gcagcgatgg
     2881 gttccgctag cggcttattt gattgttact atgctctttg ttaaactata tgtaccaact
     2941 cgtttgaaga agatgtcaga ctgaaaacaa agctgtgtag ggtaaaaatg agaatgaaaa
     3001 atagagcgtt gacagtaaga atgctgttcg ttctattttt tttgagaaaa gctggttttt
     3061 tgaatggcaa ttaaatgaaa aagaacgtat atgtgcttga cgtgtagtgt tcaggcgact
     3121 agaataataa gtactgcaaa acaaagagta agaggacggg agaatgcatg atggagtttc
     3181 agttagacca agagtggcgg ttacgtccca tcaaaggcga cacaggaaaa acatacatag
     3241 gcatgcgtga tgaggacaaa gtctttatta aacgaaacac gactccgatg ttggctgctc
     3301 tttcaaggga aggaattaca ccaaaacttg tttggacaaa acgaacgggg aatggcgata
     3361 ctttgacagc acaagagtgg ctggaaggtc gcttgctgac tcctaatgag attggtcaaa
     3421 gaaacgacgt gattgatgtt ttgtaccatt tacatcattc aaattctctt aaaaccatgc
     3481 tgacccgtat cggtggtcgt gtcatgacgc cggagaagat gctgcaaaaa tatgaaaaag
     3541 aattaccgga agaactccag aaaaatcaat ttctgcaaat tgtccatcgt tttcttcgtg
     3601 agcagatccc gtcaatagat aaggaaaact tatcagtagt acacggagat gtaaaccatc
     3661 gtaattggtt agtatgtaaa aattatttat atctagtcga ttgggattca atcatgtttg
     3721 cagaccctgc ggtggatatt gcaacgattt taggaaatta tgtgccactg tctagctgga
     3781 atcagtggct gatcagttat ggcatacgcc cgactgacga aatgcttgaa aaatttcatt
     3841 ggtatgcggc catgaatttg ctccaagaaa tcaccagata ttgcaaacgt gaagatcatc
     3901 gacggatgaa cgaagaaatt ttacaattaa aacgaatttt cagtggctga ggtttggccc
     3961 aaactctgcg gagatttggc cagacctttt taattagagg aaaggaatgg agataaataa
     4021 tgcgtgtccg aaatcgtccg ggagcagcgg aaatgttagc ggctcacccg aattttgtaa
     4081 ttagtgatcc aacattgtgg aagggaaaat ggaatgaatt gtttgaaaac gatcatccaa
     4141 tccacattga gattggtatg gggaaaggcc agttcatcac tggaatggca aaagcccatc
     4201 ctgaaatcaa ttatattgga gtagaaatgc aggtcagtgt tgtttcgatt gcactagaca
     4261 agttgatcga acaaccgctt cctaacctga aacttctaca tgtggatgga tcagcgttga
     4321 cagagtattt cgctgacagc gaagtagatc aaatctatct gaatttttcc gatccatggc
     4381 caaaaaaacg tcatgaaaaa cggcgattga catataagac ttttcttgct gtagatgaac
     4441 agattcttcg tccaaatgga gaaatccatt tcaaaacaga taatcaagga ttgtttgagt
     4501 attctctggc aagtttttct caatatggca tgatcctcaa acaggtatgg ttagatttac
     4561 atcaaagtca atttgaaggc aatattatga cggagtatga agaaaaattt tcttcaaaag
     4621 gtcaacgtat ttatcgagta gaagctcaat ttcaagataa gaccaaaaag aaaagtctgt
     4681 gaaactaact ttctgctttc ataaaaacat taatatccat aaataaatct cagaaaatga
     4741 acattacaga tgtaatgtat tttctgggat ttatttgatt taaaatgtat ttatttcatc
     4801 ggtttttcca caaggtggaa tcaattggaa gcgagaaaat gaaaataatc ctttgtcggt
     4861 gatcttcaat tcgggtatga cagataatgt taagaaagaa agtgtcaaaa aaggatcaaa
     4921 ggttttagct gctcctagtt gatgtgcttt agttattaat tcattcaact gttcatagac
     4981 gatcgaaaaa ggttcctgtg tcatgattcc gcctatagga agagaaacac aagctatttc
     5041 ctgctgtttg ttgacaacgg tcatcccgcc acctttttga atcaaaagat tagcagcata
     5101 cagcatatcc tcgtcgtttg tcccaacgac aactagatta tgcgagtcat gggcaatcgt
     5161 tgtcgctatc gctccttgtt tcagctgaaa tcctttcact atgcctaatc caatgtttcc
     5221 cgtttgatgg tgacgctcga tcaccgcaat ctttaattgg tctttttcga ttgaaggaat
     5281 gaatcgttcg ccttgtcggt ccactttttc taccaaatct ttagtcacta gactattcgg
     5341 gataatttcg atcacatggg cttgtgaaga agaaagaagc agctctaacg aatgtggatt
     5401 taatggttga gcgtttagtt ttggcagaca acacgtatat gaatttgaat gatcagcaaa
     5461 taaatggtct tggagttgcc cagattgaac aactaatgtt cccttataga aaacttcatg
     5521 aattggcagc ttggtaagat cgtctgttaa gaagaaatca gcttgatacc caggggcaat
     5581 cgcacctagt gttttcaaat caaaacattc tgctgcattc aatgttgcca tctgaatggc
     5641 ttgtaatgga tcgattccaa gtgcaatagc ttgacggacg atatgatcga ttgatccttc
     5701 ttttactaaa tcattgatca gtttatcatc ggtgacaaag aagcatcttc ttgaattttc
     5761 aggtgtgatc gccggtagta gcgatgccag atcttttgca acggtccctt ctcgaaccat
     5821 taaatacata ccaagatcca aacgttcttt agcttctttt gcagttgtag cttcatgatc
     5881 cgtccgaata cctgctgcag gataaacatt taactcttcc atgccgatac cagctgcatg
     5941 cccgtcaatt tttttcttat gctggtgcat caattggatc ttttctagga tgtctgtttc
     6001 attatttgct actgcttgat agttcatgac ttcagctaag cctaaaactt cgggtttttg
     6061 taaaaaaggg gctaattttt ttgcatttaa atttgcacca ttggtctcaa aaggggttac
     6121 aggtacacaa gaaggtagca taaaaaagat attcatgagt gtttgtcttg catcttctat
     6181 cataaactca agacctgtac tcccagcaat gtttcctatt tcatggggat ctgtcactac
     6241 agtagtaact ccatgaagca aggatacttt tgaaaattcg cttggtgata aaagtgagct
     6301 ttcgatatgc acatggccat cgatgaatcc tggcactaga tattgcccat tagcattatg
     6361 tacgcttttt ccttcgtatt caccgattcc tacgatcatt ccttcgcaga ttgctacatc
     6421 tttttccatg atttctttcg taaagacgtt gacgatctta gcatgcttga tcactaggtc
     6481 agcaggcttt tttttacttg ctacttcgat atatttcctt aatgttgttt ccaatggagt
     6541 gcctcccaag ataaattttt ataagtataa cataaattcg gcttttgcca atattttttt
     6601 gtaaattcat tttatttgaa acaaaaagat caaaaagacg tatgtttttt gtggagggga
     6661 gaataatgga aataaaacga ttatcggatc aaatgataag tgaaagtttt gccaaacatg
     6721 cccaagacgt atatatattg ccagcttcag aagtatttta catctatttt cgctatggaa
     6781 aaaaacgaac attctggaag aaagtctctt cggcagatgg acagcaactg atctcaagat
     6841 ttaagtattt gggacagatg gatatcggag aaaaaagaaa agctcagtta ggtgcattta
     6901 cttattcttt agaaaaacag gaagttcgtc tgcggttgtc ttctgtagga gattaccttc
     6961 aacgagagag tttagtgatt cggctattat acggtacttt tgaaacaccc catcgttgct
     7021 ttagttctcg agattttgca actattttac agaaaaccgt cagacgtgga ttataccttt
     7081 tcagcgggac agtcggatca ggcaagactt ctctaatgta tcggttggct ttggaagaaa
     7141 aattgcaagt gattactatc gaagatcctg tagagataga agaaccgttt tttctgcagc
     7201 tacagatcaa tccgaaaata gcacagactt atgatacact tttgaaactc gcattacggc
     7261 atcgtccaga cttgctaatc attggcgaga taagagatga atcaactgca catgctgcgg
     7321 taagagcagc actaacaggg caccgggttt ttgcaaccgt acatgcacgc aatcttgaag
     7381 gtacattggt cagaatccat gaactgacaa agaaaaaaga agagctatct gaatgtttag
     7441 ctggtgtcat ttatcaagaa cttttggaag atgccaatca ccatccttct gttttatgga
     7501 gttatcaatt tatggaggaa gaatcagtaa aaaaatcatg ggaggaaagt tatgaagaag
     7561 ctcaggaaaa tatttggcag tcagaactat tctaaaggat taacaagaag ccagcaaaac
     7621 cagtttgcac aaaccatggg agaactgctt ttatctggtt ttagtctgca agaatccctt
     7681 tatttattac tcacgatccg ttcgtttcca cctgttattt tggcaaatat ccaacatcag
     7741 ctaaaggaag ggcaagcgtt tagtgaggta ctttcgcaaa taggatatgg agcagagcaa
     7801 ctggtacaga tcgagttagc tgaacgtcat gggaatctgc cgacaacttt gttggaaatt
     7861 gccaaacagt ttcggcttgt tgaaggattt cgcaatgatt tgaaaaagct gcttgcatat
     7921 ccgatgctat tgctcacctt tttgctaggt atcttgttta gtatgcggtt gttcgttttg
     7981 cctcagctta ttggttcagg aatggtcatg caagagcatt ggggtattca gctgatacaa
     8041 gtctttcatt ggtatgttct actattcatc ctagcggtta ctctattggc tttactagta
     8101 aggtatcgtc tgatgaagtt gtctcctgtt gatcagagtg aatggataag cagacaatta
     8161 ttgattggac cgatatattc cttgtatcaa tcggcttatt tgtcactaga gattggaaag
     8221 ctttttcatg aaggtttcga attaaggcaa atcatagtct gcttaaagga aacgaaacaa
     8281 gaaagtctga ttcagcatct tgcctctaag ttgattcaag gattggaggc aggtatgtca
     8341 ttggctgagc agttccagaa ttatcgtttt ctatcagaag aattcagtaa aattgtttta
     8401 cagggagagg caaagggaag tttaggtaaa gaactgattt tttacagcga atggacaaga
     8461 aaacagcttt tcaaacgtct tcatcactgg atgcatttta ttcagccaat catttttttg
     8521 ggcgtagcgg ctttgatttt attagtttat gccgctgttt tattacctgt ttacggaagc
     8581 atagaggagg ttttgccatg aaaaagagaa aaatatctta ttatagcggt tttacattga
     8641 tcgaaatgct aatcgtctta ctgatcatca gtgtattagt gttgttgttc gtccctaatt
     8701 tgtcgcgtta taggaatcat gtagaccaag aaagtcgtga agcgatcatc cagttggtag
     8761 acacacaaaa agagctctat gcgcttcaaa ataatggaag agttcctact gtggaggaat
     8821 tgttgaatga aggctatatc aaacgagaac atgcagaaat ctatcagcgg ccttgaaggt
     8881 aaggttttag catcaggata tacgttgttt gaatcactct tactgatttt attacttaca
     8941 acgatccttt cttttccagt tctcgcattt tctgcatgga agaatgaatt ggcagtctat
     9001 caatttttcg gccaatttga aaaacgaatc tattctacgc aaaaaatcgc tattgtgaat
     9061 cagatccaaa caggctttta ttggaatagt gaagagaatc aaatcatttt tcatgttccg
     9121 aatccagata aaataaattg ggaagtatta gatattcctg atgagattac cttgaaacgc
     9181 catgcttcta ttacttttgc tgctagaacc gggaatgaaa gttcactaaa agcttatcaa
     9241 ttttattggg aagaaaaaga gcggacgatt acgtatcaat tccagatagg gagtggtcgc
     9301 tatacaaaaa gaattgaata aaacaggatt tgttttgttg gagtccctta cagcgcttat
     9361 gatcgcttct gtctgtgtct acttagtgag ctttggccag ctgtttttgc ttcaggcaga
     9421 aaagaaaagt ggggaggaac tgctatttat tcgaatgatt tatgaagata tcaagacgca
     9481 gcggatgtat cacaaaagac cagagcgaag ggaagaaatg atcccatacg atatccaaat
     9541 tgtcgataat aatggagtaa tcgaaaaagt gtttgtcaat atggagaaag aagcgatcag
     9601 tattgaaaaa acaagtaatt ccagcattta cgctgattga atgtctaatt ggactgatga
     9661 tattgagcag tctattgctc acattcaatc ttttgattca gcaaaccatc caagtaagtg
     9721 aatttttgca gcgtaaagat caaaaagagt ggctaatatt tttagcacaa ttagaagaag
     9781 aactcaagag cagtcacaat gtctctgtga aaaatcagca actgcactac acgatcgagg
     9841 ataaacagta tatcatcgaa agatatcagg caatgatacg taaaagaaga acttccggcg
     9901 gacatcaacc tatgttaaca agtatcactg agcttcaatt attggaaaaa gaacacacaa
     9961 tttcttttta tgtacacttt gaaaatggag aagatggata tgccatatgg acaaaaaacg
    10021 atcagtagaa aatgttataa gggctctatt ttattttctg tcttgctttt atttctttta
    10081 tgtacgtttt tattgatggc agcattagaa agctaccgtt tgtcaatgga tcttaatata
    10141 cgaatcaaaa attattatat agcaaaaata atgaagcaga tggctttatc ggaacagtct
    10201 acgcttgccg aaagttcgga aggagttttt tactacacaa ctggatctgt aacatatcag
    10261 tgggtacaac agtccctatt cttagaggtg gaagtctcac cgtttatctt tcggttcaca
    10321 gaagaagtaa aaaatgatac agatactagt acagaatgat ggaatgttcg tgaaaacgcc
    10381 ctcttttctg ttataacact gtttttaata aaaaaatgtt tgccgtttca caaataaaaa
    10441 acagtgaaaa cgattgaaat tttgtcaatt gttcgataga attaaacagg tgaaaaaatt
    10501 gttataatat atctagaaat gaggtgcacc atgttaccag aaaaaatgga acaagggttt
    10561 aatcaaaacc tggaagctat tcagctttta cagaatgcat tgggaacttc ctttttagaa
    10621 gcctatgtcg aaaatgcaga aaatttaata gatgattatc aagtacgtgt ggtggatgga
    10681 gtgccgacaa aagagacaac gcaacggatc acagctcttt atgaagagtt gaagaaactt
    10741 tctttcgagc cagaagaatg gcgcagactg tctcaattac ttttattgaa gggcagccag
    10801 acagaacatt tgcaggcgaa ccatcaactc actcctgata gtatcggttt tttatttgtt
    10861 tttcttattg aacaactata cacaaataaa aaagcaccgg ttaagattct ggatattgcg
    10921 gcaggaatgg gcaacttgtt attaacagtt cttttaaacc tatcgaatgc ggggtaccaa
    10981 acagaaggaa ttggtgtgga tattgatgat acattgttgg ctgtcgcagc ttctacaagt
    11041 gatttgacac aagcaaatgt tcaatacttt catcaagacg ggttgcagga attattaata
    11101 gatcctgtcg attttgcaat cagcgatttg ccaatcggtt attatccgaa tgacgaaaaa
    11161 gcaaaagagt ttttaacaag taccgaagaa ggccatagtt atgcacatca tttattgcta
    11221 gagcaatcaa tgaaatatgt gaagccggat ggttttggtt tgtttttgat gccgagtgga
    11281 tttttagaaa cggatcaaag tgaagaaatc aaaaaatggt ttaaagaaga aggttatctt
    11341 caaggaatga ttcaattgcc agatgaattg ttccgtaata aacaatcgca aaaaagtatt
    11401 ttgatcttac agaaaaaagg accacaagca caacaagtaa aagaagtctt gcttgtgaaa
    11461 cttgcttcat taaaagaacc agagaaggtg acggaatttt tcaacgagtt taaaaactgg
    11521 aaatcatctg ctctgtaatc attcaacgag aaggagagtc atatgtctaa aacaattgca
    11581 atcaatgctg ggagttcaag tttgaaatgg caattatatc aaatgccaga agaaaccgta
    11641 gttgcgaaag ggatcgtcga acgtatcggt ttacaagatt ctatttttac catcaagtac
    11701 ggcgataatc aaaaattcga acaaatcatg gatatcgaaa accacgaagt agcagtaaaa
    11761 atgctgttag atcagttgat tgaactgcaa attttaagct cttatgatga aatcaccgga
    11821 gtagggcacc gtgttgttca cggaggagaa acttatgggg attcagtagt gatcgatgag
    11881 aaagtaatgg accgcattgc tgaattagca gaatttgctc ctttgcataa tcctgcaaac
    11941 ttaatgggga tcaaagcgtt taaaaagatt ttacctgaca ttttgagtgt ggctgtattc
    12001 gatacatctt tccactcaac aatgccaaag caaaattatt tatatagctt accggtgtct
    12061 tattatgaag atttcaaagt aagaaaatat ggcttccatg gcacaagtca tcgttatgtg
    12121 tcggaacgtg cggcaaaatt attgaacaaa ccgatcgaag aactaaaaat catcacttgc
    12181 cacttaggaa atggtgtatc gatcacagca gtcgatggag gtaaatccat ggatacttct
    12241 atgggattca caccattagc tggggtgaca atgggtacac gttcaggtga tatcgatcca
    12301 gctgttctcc catatttaat ggaaaaatta gagattgata ttgatgaaat gatcaatgtt
    12361 ttgaacaaga aatctggtct gctaggccta acaggattgt ctagtgatat gcgtgatctt
    12421 gaaaagaact atgaaaaaga acatattcgt ttagcttatg acattttcgt ggaccgcatc
    12481 cgaaaataca tcggcggtta tgtgacaacg atgaatggtg ttgatgcaat tgtctttact
    12541 gctggtatcg gcgagaatga cggacatgta cgtagtgaaa tcatcaaagg tatgtcttgg
    12601 tttggttgtg aaatcgatcc agcgttgaat gaattgcgtg gtgaagaagt agatatctct
    12661 acaaaagaat caaaagtaag agtattagtg atcccaacag atgaggaact aatgattgcc
    12721 cgtgatgtgg agcgtctgcg cggctaagcc aaagcattcg ttaggataaa agatgaaacg
    12781 catgcaagag ggaattcttg catgtgtttt ttttgcggag aagaccgcaa aatggtaatt
    12841 taacttcaat ttcccttctt ttttacagga tcttcttagg gaattttgct tggaaaagtg
    12901 gtcaaaaggg aaaagactgg cattttattt attgattttc aggaaaatcc caatatttta
    12961 ttttcattca tctttataag ggacattaca tatgtaaaaa gaaaaaacat ttttagatca
    13021 gcctcgtggg ctgtcgactt tattcttcgc tttatgttta gctagaaaac cgatccgaaa
    13081 cattcattga tcgaataata aaaacttgat tttccacctg tactttgcct gaatcgtgaa
    13141 agcacaggta tgaaaatcaa gttttttgtt gcgaaaaaac gtcctagtca atcagttggc
    13201 cgagcggctg gcggctgatc tgctcctgat caataacgag tgcttcttgg aagtgttgct
    13261 gcaaaaagta gcggtcatgg ctgactgcta ggagcgttcc ttgatagtct tgcagcattt
    13321 cttcaatttc ttctcgagca tagctgtcga ggtgattggt tggttcatcc aaaatcagga
    13381 cgttgatttt ttctaaaaag agtttcagca acgccaaacg gattttttca ccaccggata
    13441 aatctttgat ccgtttaccc acatcgcttt gataaaagcc acttttggct aattgccgtc
    13501 gagcgacttc ttcgtttcca gtcaatgtga tggcgtaagc cagcaaacgg ctatcgggat
    13561 cggcaaactg cagctgctgc ggcaaataac cgatttgcaa gctactacct aatttgatgg
    13621 tgccaccgta aggcttttcc tcaccgagta ataatttgag taaggttgat tttcctgcac
    13681 cattgtcgcc gatgatggct aaatgatctc cacgatagac tgcaaaactg ctgtctttga
    13741 atagtacttg atcagcgtac tgctggtgca agttttgaat gatgaaaact tctttgccag
    13801 attttccacc attagacaag ctttgcagcc gatttttcgg gggaggtggc ggtttgacga
    13861 gtgtcagttt cgccaggcgg cgttccagtt ctttggcttt tttgaagaag gactcattgt
    13921 ctccttcatg tgcccactga cgaaaacgac gaatcatgac tttcaaccgc tggacttctt
    13981 tttgctgcag ttcgtaattt tttgtcagct cagccaagcg agcttctttc aagactcgaa
    14041 aacggctgta gtttccggga tactcaatca atgcaccttc ttccagttcc catgtttttg
    14101 tggtgacttg atcgagaaat tgccgatcat gagaaacgac cagaaccgat ttttttgtat
    14161 tctgtaaata gttttccaac cagcgaatac tgttaagatc taaatgattg gtcggttcat
    14221 cgagtaaaag taaatcggca tcggctgcta aaattttggc taaagcgact ttaactcgtt
    14281 cgcctccgct aagttctgtt aaccgtgctt cctgacagtg tggaatccct aagccttgca
    14341 agatcgctgc aatccggtct tctaatacgt aaccgcctag ttcttcaaag cgatgctgca
    14401 gctcaccgta acgggccaga cacttctcta agtttgtatc tggttctgtc atcaatgctt
    14461 cgtagcgact catttgctgt ttgaggtcct gtatctcagc ggcagaatgg agcaagtagt
    14521 ggttgacggt cgcttcggaa actgtaagtt cttgttcgac cgtcccgatc tttaagcctt
    14581 ttttgcgact gataacaccg ctgtcgacac cttctctgcc ggtcaagatt tgtaaaaagg
    14641 ttgatttgcc agttccgttg atatcgacta aggcaatttt ttcaccagct tcaatttgac
    14701 aggataaatc ctcaaataat ggattcccgc cgattgtttt tttaatagaa attgcttgta
    14761 tgatcatgat ctctctcctt gagaccactc aaaaaggcca tggttatccc tggcctgtgt
    14821 tatttaaact cagattatca tgagaaaaat accttgagat ggccattgat tttacgtgaa
    14881 ttcaactgtt ttggacaaac ttgtcccgtc aacagcaact gtaaaaccaa ttgcatggct
    14941 aaatgcgata tggtatcgtt ggtaattttc attacttgtt attttagtca tgcgcatcat
    15001 agtattttcc ctccttcgtg tagttgtctt ttccagtgta cctgaaagtg tcagaaagtt
    15061 caactagcgc tgacatttac ttaatattta tcgatgtgta attctgtcag agagcttttt
    15121 ttatctgact gagggtaatg tctcgtggac cacatcattg tattctgagc taaaccagcg
    15181 ttcaggcgaa atcatatggt gtttgataaa aaactttgtc tacatgactt ccttttgcca
    15241 tttgcttaat accgttgcat taaacagctc ttctctttgg aaatctcacc aacatcgcgt
    15301 tcaggagtat cgttgtggtt gcaatggcaa gcggtagcct ttcatcaatt cttgaatgat
    15361 ttaccgaatg tagcacttgc cttctcgcgg tacccgaacc cgaaataaaa ttttagtttg
    15421 gcgataaaat cggtatccgc catcgatact tcatattttt tcgtatcaga ttcatcggaa
    15481 acttccacat tgacgaacca ataagctttg gcatgcttag gtcgttttaa accacaccag
    15541 ggcgactaat cgccctggtg ttcacttttt ttattttact tgaactcatt gactgttttc
    15601 cctttatttt aaacattata aagaccgatg agtgcttgcc gcgtcttttg tttttatcac
    15661 ggttttttgt gcaaaaaaac ctgccgatag ttggcaggtc gtctacgtgt taagctttgg
    15721 tgtggtcttt agctggcaat tggttttcta aatcagttcg gacaacaagc acatcgcaag
    15781 ctgcgtttct aataacatat tcagaaacag aaccaataaa taaacgttct acagcattca
    15841 atcctgtggc tcctaacata atcaaatcta cttgattatc ttctgggatt tctctggcaa
    15901 taatttgttt tggtgagcca tattctacga cagaggttac attattcaag ccagctttct
    15961 tcgcattgct ttcataatct gccaatgttt gctttgccat ttcagtagct tgttctgcaa
    16021 gcattccatc aaaggatgaa acggtttgga aagaacgtgt gtcaatgaca tgtgctaata
    16081 gaagttctcc gttatttcgg atagctacat tgaccgcctt tttaaaagct aattcagctt
    16141 catcagaacc atctacggca accatgattt ttttgtattg ttgtaacata agcattcact
    16201 cctcaagtca atcgtattgt actcttttat tgtagaacac tttagggaaa aagagaatta
    16261 tttactgctt tggttcagca attttttttg caaatcggat actgaaggtg gcagaaacaa
    16321 taattacgat cagtaaaaag accaaggcaa taaagcgttg attgaaaaaa gctgttaaaa
    16381 tacccattac gcctaaaaca gcatatgcag caccgtaaaa ttgtaggaat ccttgatttt
    16441 tatcattttt gttgatcaat acaaaaaaga tatcctgttt attaaataaa taatagctaa
    16501 cgaataataa tactaaagtg gcgataatca ttaaaatcct aatcatgaac aaatcctcca
    16561 aaataagcaa caaatagaaa aagcaatttg ccaagagcaa attgctttat tctttaaaag
    16621 atggtaatct ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg aattcaagca
    16681 ggtgggtccc atggtcgtag cttcgaacta ccaaatgata aggcatgtta ccagcagtga
    16741 cacaagacgg gtcccaagat atcaataaaa atgttcggtc aacacacaaa gaggacaatt
    16801 cgaacatcac agatttccca cctctatagt agcatatgaa tttaaaaaga gcaatgagag
    16861 agctcgcaga aacagaaccg ttttcttgtc tagttttttg gaggatgctt tttccactct
    16921 tttatacggt aatactgttg acccagagcc tgttcatatt tccctgtatc ttttggctga
    16981 taatactgag cattcttcag tttatctggt aaatattgct gattgaccca agcctgatca
    17041 aaatgatgag gatactgata acccacgcca cggttgagtg atttcgcacc tttgtaatgg
    17101 ctatctctca aatgatcagg aacatctcca gctttgccct ctcgaatatc agcaagtgct
    17161 gcatctaatg ccatgtaggc agaattggat tttggcgaca agcaaagatc gacgacgacg
    17221 tctgctagtg ggatacgtgc ctcaggcaat cccaattttt cagcagctaa tacagcgttc
    17281 actgtacgag ccgcagcagc aggattacct aaacctatat cttcataacc gataaccatc
    17341 agacgccggc aaatacttgc cagatcacct gcctcaacta aacgggcgag ataatgaagt
    17401 gcagcatcca catcacttcc acgaatcgat ttttgaaaag ccgaaataac gtcataatga
    17461 gcatctccat ttttgtcatg ggtaagagct tttctttgga cacattcttc aatgatcgac
    17521 agtgtaagat gaatccttcc ttctttatct gaaggagtcg atagtgtagc cagttccagc
    17581 ccattcaatg cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag aagagcatct
    17641 tcgtctaact ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg
    17701 actgccaact ggatgtcttg ttctgttaat ggtttgactt caaagatctg tgtacggctg
    17761 cggatcgctg gattgatggt aatataagga ttctcagtag tcgctccgat caagatgatt
    17821 cggccacttt ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa tcggtgtact
    17881 tcatccaata agaggatcac tgtaccactc atttttgctt cttccgctac aacttgtaga
    17941 tccttttttg aatctgtagc ggcgtttagc atgcgaaatg cataattagt agagccggcg
    18001 atcgcacttg cgatactcgt ttttccagta ccaggaggtc catatagaat catggaagag
    18061 agcatgcgag catcgaccat gcggcgtatg atttttcctt ccccgactag atgctgctga
    18121 ccaacgactt catcgatcgt tcgaggtctc atgcgataag ccaatggctg ttgcatagga
    18181 ttttcctcct tcttgttttt acgtccctta attatatcat atttagcaag atgcctagaa
    18241 agaacagcct tctgttgttt tgttttcata gcttatccaa ctaatattgt gtatgataaa
    18301 gtaggtgaga aaaatgacgg ataaacaaaa gatagatgaa tttttttgga ataaaacgac
    18361 gccagaagtg gcacgggatt tattggggat gtatttggag catgacacac cagaaggaag
    18421 attagctgga tatattgtag acgcggaagc atacctaggt ccagaagacg aggccgccca
    18481 tagctatggt ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat
    18541 ctacctttac acgatgcata ctcatcggat tttgaacatc attactcagc cagaagggat
    18601 tcctcaagga gtaatgatac gtgcaatcga acccgccgca atgattgatc aaatgagtaa
    18661 aaatcgtggc ggaaaaactg gtccagatat cagcaacggt ccgggaaaat tagtggaagc
    18721 attagcaatt cctcaagaac tttatggtca gtctatagca gatagttcgt tacgcttagt
    18781 ttttgagaaa aaaaagacac caaaaaagat caatgctttg cctagaatcg gtatcccaaa
    18841 caaaggtgta tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc cttatatctc
    18901 tttacaaaga aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga
    18961 aggatctatt aacatattta gatgaaataa ttgaaaaaca agtgacagac tatgatgtag
    19021 ctttagattg ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa
    19081 ataaagcaca atttgagatt gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg
    19141 aatttgaaga cggcgtactt ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt
    19201 atctagcagt aatcccttat gaaggaaaaa aagggttggc aaaatcagta gctgattctt
    19261 tagtggaata tctaaatgaa gtattagctc aagggcaaag cgatttgctt gattttttgg
    19321 atgaagacga tgacgaagct gtattcgagc ttcactggga taatcaacaa ctagcagaat
    19381 tagtagaagt aaaacagcaa aatgaagcag atacttattt accgtaccca agttattaag
    19441 gagtgacaga tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag
    19501 cagttgctaa tattttgatg gaggcagggg cgagtggtgt cgcgattgaa gattctttag
    19561 atgttgaaca ttttcagtcg gatcaatatg gtgagatttt agataaaaat gattttacat
    19621 cgctgaaaga aggcgcacta gtcatggctt attttccgga aacgactttc ttaccggaaa
    19681 tcttgccttt tatcaaagat cgtatcacac aattacctga gtttggtcta gctattggaa
    19741 aaaataaagt aacagttagt gaagtagagg aaagcaactg ggcaacagcc tggaagaaat
    19801 actatcatcc ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg gaaaactacc
    19861 agacagaaga tcctctagaa aaaattatca cactagatcc tggtatggca tttggcacag
    19921 gcactcatcc cacgacttcc ttgacattga cagcactaga aacagtcttg cgtggtggtg
    19981 aaacattgct agatgtggga actgggtcgg gcgtgctaag tattgcagca aaatatcttg
    20041 gtgcaaaaga tgtctatgcc tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa
    20101 atatggatat gaatgaaata gccaaagatg tccatgtatc agctaacgat cttttgacag
    20161 gaatcgagat tgaaagtgat gtaatcgttg cgaacatttt agcagatatc atcttattga
    20221 tgatcccaga tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt tcaggaatca
    20281 ttgaagagaa gaagcaaatg gttctggatg ccatgttaga acaagggttt atcgtggatc
    20341 aaattttcca gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag gtggaataga
    20401 tgcagcggta ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt ttagaagggg
    20461 aaaactatca ccacattgta cgggttatga ggatggaacc cgaggatgaa gtttttttgg
    20521 cattcaatga tcaaatagcc attgttgcag aaatcacgga gattactgaa gaggctgttt
    20581 atctgaaaga aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg attaccatag
    20641 cgagtggcta tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc actgaattag
    20701 gagcgcaagc ttttcttggt ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa
    20761 aacgtatgaa acgacaacaa cggcttgaaa agattgctca agaggcagcg gaacaatccc
    20821 atcggcagat cgtgccatca gtagagttat tggaaacgaa acaagattta tttgagcggc
    20881 tagatgatta tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat
    20941 caaatgcagc caaaattctt agtgaaacaa aaccagatag tcgaatctta gctgtctttg
    21001 gaccagaagg tggattatcc cgcgatgaag tcgattattt tgtagaacac aaagggatac
    21061 tttgtggatt aggaccaaga atccttcgta cagaaacagc acctctttat ttattaagtg
    21121 ctgctagcta tcaatgggaa ttaatgaatc cttctttcag ctaacgttct tcattaataa
    21181 aagagtatcg acagaaagaa ttgaaaaaat agtcaacaat ttgtataata gaaacaatta
    21241 tttatacaat ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa catttccgta
    21301 aaaaaacgta taactgcacg gattttggga caattatcaa acagatattt gtcccgattc
    21361 ccgttttacc gaaaaaagaa agggggatcg attcctgtga caaaagaaga aattttaacg
    21421 ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt
    21481 gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa atcaggcgaa
    21541 ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa aatggatccc
    21601 catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat cactttagaa
    21661 gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt aaccaaatta
    21721 gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg aaagatgctg
    21781 ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag actacataac
    21841 atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca ggaaacttta
    21901 gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg ggaattagaa
    21961 gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca cttgatgcag
    22021 acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat tcgaatagcg
    22081 acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat ttattcaatc
    22141 tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt gctagctatt
    22201 cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat ccacacaaaa
    22261 tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc taatatgtac
    22321 caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat tcaaatccga
    22381 acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc ctataaagaa
    22441 ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg gttccatgag
    22501 atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg cgtaaaagga
    22561 gatatcttta gtgataaagt ctacgtcttc acaccaaaag gagacgttac tgagttacca
    22621 aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg taacaaaacc
    22681 actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa aaacggagat
    22741 atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg gttgaaatta
    22801 gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca agatcgagaa
    22861 gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct aggatttacg
    22921 cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt taattatcaa
    22981 acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt gacgatggcc
    23041 aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca
    23101 gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt
    23161 ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg ctgtaatccg
    23221 attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc cattcatcgt
    23281 cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc
    23341 gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga aatttacggc
    23401 tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat
    23461 ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca tttgacggtt
    23521 ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc agtacctgat
    23581 gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag ttgttcaacg
    23641 ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg gctacggcct
    23701 acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact atctggtaaa
    23761 aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata ttagtgtaga
    23821 agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa
    23881 aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc ctttatacga
    23941 atcattcaat gaaggaatca gaagtcgcgg catcacagtc gctaccggac aattcggagc
    24001 agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg attctaaaaa
    24061 caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct taagttagac
    24121 tgataaactc taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt
    24181 cggaaaaaaa gttaagctta aattatacaa gttatttttt ataggaggtt gctgaagatg
    24241 aaagaagtac cagtagtaga tatcaaaatc acagatttaa aaaaattgta tcaaaaagga
    24301 tacaacatgt tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt
    24361 ttcaataaag aagctatcac gatctacgga tcatcagcag caaaagtatt ttatgatcca
    24421 aggaatttca aacgaaaagg tgctatgcca aaattagtat tgaagacact ttttggtcaa
    24481 ggtggagtac agactttgga tggagctgct catcaccatc ggaaaaatat ttttatggat
    24541 ctaatgacac cggaaagaat ggaagattat catcgtatct tagataagaa tctgacacaa
    24601 gcacttgaag cacagcatgg tcaatttgaa ctatttgatt taagtaagat ggttttcttt
    24661 acatctattt gcgagtgggc aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa
    24721 aaattagcag aatatcaaat ctctatgatc agcggaacgt ttacttctcc gattgatcac
    24781 ataaaaggtg tcgagaatcg caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa
    24841 gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc
    24901 acagatttag atggtcagct tttacctctg gaagtggcag ctgttgaatt attgaacatc
    24961 atccgtccaa cggttgctct aactgtgtgg gcggcattga tgggacatgc acttttcagt
    25021 agaccagatt tgtatcagca attaaaaaat gacttttcga cgttacaaga tccgtttatt
    25081 caagaaatga gacgctatta tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa
    25141 gtagaagtag atggttatcg tataccagaa ggcagttggg ttattttaga tttatatgga
    25201 acagaccatg atgaacgaac agttgaagcg ccagattcct ttatgatcaa acggtatgta
    25261 gggaaagcaa aagatatttc ctataaagaa gaatatgaga tgattgcaca aggcggggga
    25321 aattttcgac aaatgcatcg ctgtgcagga gaatggatca ctcttcatag tctacgtgtg
    25381 ttttccgatc agctagtaaa taagtttgag ttctctgtac ctgaacaaga ttggacgatt
    25441 ccattcaatc agtttccgac ttatccgaat agccgagcgc tactttacaa gaattagatt
    25501 agcataaact tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt ttttcatatt
    25561 actataaatt gataaggtca attgatcaaa atccacgaaa tgcttcatca atggtttgat
    25621 aatcttcatt ggataaatgc aaatctaaag cttggacatt gtcagatact tgctctggac
    25681 gcttagctcc aggtataact acagacactc gtggattctt catgtaccaa gctaaaacaa
    25741 gttgcgcagg agtggcttgg tatcttttag caatggggcg aagtacatcg acagcggtta
    25801 aaatttctct gaaacgttct ccttggaaat ctggatttct ttttcgagga tctccttcac
    25861 caaattgttt ttcctcacca agttcatatt ttcctgtcag caaaccggaa gcaagaggaa
    25921 aatagggaac aaaactgatt ttgttttttt caagataagg gaataattct ttttcagcct
    25981 gccgatggat caaactgtat ttatcttcta ctacatcgac atatccatct gcattcgctt
    26041 ctttcaattg ttctaacgtg aagtttgaca caccgactgc tcggattttt cctgcttctt
    26101 ttaattcgtg caatgtagca actgattcat tttttggtgt gctttcatcc ggaaagtgga
    26161 tataaaaaat gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc accgattgtt
    26221 tgagaaactc aggagaatta tcaaatgttc gccctttatt tggtacatga gcagcttttg
    26281 tagcgatgac gacacgtgag cgatcatatt ctttttcctt cagtacctcg ccaatcagtt
    26341 cttcggaacg gccgtttccg taagcataag cggtatcgat caaattgatc ccgctattca
    26401 aagctgtacg aactacttgt tttcctgtct catcttccaa accgtcaaaa agattatgtc
    26461 ctccaacggc atttgctcct aatccaagct gttctgcgta gacttgtgaa tgtccgattc
    26521 ttatttctgc catagctgat tcctcctttt tgtctagtct aaaacaaaat accaaaaagc
    26581 tgaactaaaa cgtttttgtg aggggaaata gtcagatatc tcttgacttt gagactggtt
    26641 tttagtaggt tataagtaat atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt
    26701 tacaagagag gatcatcgag gctgaaaatg atctaaagtt tcattagcct aaagacactc
    26761 tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga
    26821 aatctttcaa aattaggtgg taccgtgtat gaatttcatt cacccttgtt catgatgaac
    26881 aagggtgatt tttttattta catagctaag aaagggagag aaaactatga gtttccaaag
    26941 accaaaagga acaaatgata ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga
    27001 aacagcacgt ttattattca gagattatca gtataatgaa atgcgtacac cgatttttga
    27061 acactatgaa gtgatatccc gaagtgtagg ggatacaacg gatatcgttt caaaagagat
    27121 gtatgatttt tatgacaaag gggaacgcca tgtgacgctt cgaccagaag gtacagcacc
    27181 aatcgttcgt tcttttgttg agcataaatt gttcggccca gagtatgcga aaccgttcaa
    27241 aactttttat atgggaccaa tgtttcgcta cgaacgccct caaaaaggac ggttacgtca
    27301 attccatcaa atcggagtag aagcatttgg atcagaaaac cctgcaactg atgtggagag
    27361 catggtgatg gcacttgatt tcttcaaaca gttggggatc acgcaaatcc ggctagtcat
    27421 caattcatta ggtgataaag aaacgcgtca agcctatcgt caagcattga tcgactatct
    27481 tttacctttt gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt
    27541 acgcgtattg gacagtaaag acaaacgaga tcaaaaattt gtggcagaag ctccatctat
    27601 cttagattat ctaagcgagc ctgctcaaaa acattttgat accgttgttt ccatgctgga
    27661 tgcattggct gttccttatg aaatcgacag taatatggtt cgcggactag attattacac
    27721 acatacgatt tttgaaatca tgagtgacgc accaaaaatg ggggcacaag caacgatttg
    27781 cgcaggcgga cgttatgaca atctagtgga agaactaggt ggtccttcaa cacctggctt
    27841 cggatttgca atgggtatcg agcgattact cttgactatg gaggcagagg aagtagttat
    27901 ccctgcattt aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa caaatatcga
    27961 agcattgaaa gtggtccaag cgattcgaaa ctttggtttt tcagctgacc gcgacttcat
    28021 gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa gcgaatgcca aattagtatt
    28081 ggtgatcggt gaagatgagc ttgctagtca aacgattaat gtcaaagcaa tggcaagccg
    28141 aaaagaaaaa agttttccat tagaagagat atatgaacat tttgatgaag tctatgatga
    28201 aatgacttta ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat ctattgcgga
    28261 ttagtttccg aacaacaatt agaacaaaca gttacattaa aaggatgggt acaaaaaaga
    28321 cgagaccttg ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc
    28381 gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg cggataaatg tcgcagtgaa
    28441 tacgtgatcg aagtgactgg ggaagttaga agacgcggag aaaatgcagt aaatcctaaa
    28501 atggccactg gtgaattcga agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa
    28561 accccgccat ttcaaattga agatgatcaa gcaatcagtg acgaaatacg tttgaaatat
    28621 cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca
    28681 acaaaagcga ttcgtcatta tttagatgat aatgactttc tcgatatcga aacaccttac
    28741 ctagggaaat ctacaccaga aggcgcaaga gactacctag ttccttcacg tgtccatgct
    28801 ggacatttct atgcgttgcc gcaatctcct caattgttta aacaattgct gatgaatgct
    28861 ggattcgacc gttattatca aatcgttcgt tgtttccgcg atgaagattt acgtggagac
    28921 cgtcagcctg aatttacgca agtagatatt gaaacaacgt tcttaacagc agaagaaatc
    28981 caaacttaca cagagggatt gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc
    29041 acattgccat ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa
    29101 ccggatacac gttttgatat ggaattgatc gatctaagcg atacagtcaa agaagtagaa
    29161 ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa
    29221 ggcgctgcag atcgctactc acgaaaagac atggatcagt taggtcaata tgtaggacaa
    29281 ttcggagcta aggggcttgc atggctgaaa gtagaagaag acggattgaa aggtccaatt
    29341 gctaaattca tgggagaagc aacagaagca atcattaaag caacagatgc aaaaccaggt
    29401 gacttgctga tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc
    29461 cggacacgtt taggaaaaga attaggacta attgatgaat cgaaattcaa tttcttatgg
    29521 gtaactgatt ggccacaatt tgaattttca gaagaagaag gtcgatatgt ctctgctcac
    29581 catccattca cgatgccaaa agaagaagat atcccgttat tggctgaaga ccctgcaaaa
    29641 gtctatgcag aagcttatga tatcgtcttg aacggttatg aattaggcgg aggttctatc
    29701 cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa
    29761 caaatggaag aacaatttgg tttcttgtta acagctttag attacggatt ccctccacat
    29821 ggcggtattg cattaggatt ggaccgtttt gcaatgttat tagccggaga agataatatt
    29881 cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag atgtgatgag tgatgcacca
    29941 agtatggttt cgcctttaca attgttcgaa ttgaatattg atgtaacgaa tatagaagaa
    30001 tagccgataa aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa ccttgattta
    30061 attgaaaaac actttataat gaagccataa catataaagc ggggcttttc catgaaaaaa
    30121 acattcgaaa attgggctta tcatgattac acgaccaagc tttcggtatc tatcgtttat
    30181 gcgttgatcg catcagtcgc attgaatttc ttttatcagc caggacatat ttattcaagt
    30241 ggtattactg gtctagctca gattttgacg actttgtcaa aatcactgat aggattcgaa
    30301 gtaccggtat caacaacttt gtatttactg aatatacctt tgtttgtatt ggcgtggtta
    30361 aaaatcggga agaagtttac aattttcact tttatcacag tcactttgac ctccctttgt
    30421 atccatattt taccggaaac agtcctatct accgatccaa tcatctgtgc gttgtttggg
    30481 ggggctgtga tgggactagg tattgggcta gcgttgagaa acggcctatc atcgggcggt
    30541 ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg
    30601 atctacttca atgcattgat cgtttttgtt gccggaattc tatttggctg gcaatatatg
    30661 ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa
    30721 caaaagaaga tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc
    30781 caaaactgta tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc ttataagcat
    30841 gataagcaga cagtattgat gacgatcgtt acacgatacg aactgcctct acttagagaa
    30901 gcaatgaaag cttctgatcc acaagctttt gtaagtattg cagaaaacgt ccagatactt
    30961 ggtcgtttct atgaggatgg catttagatc gagtttgaca aaaattcatg aaatactgct
    31021 gtagacagac gttcgtttgt ctgcagtttt tttctaccat agtagaatca gcaaagaaaa
    31081 actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat ttttttaaat
    31141 ttatgggatg atagaaaaaa atagtagaga aaggacacga ttctacttgc tattttcact
    31201 taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa
    31261 acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc gttcattagc
    31321 tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg gctatttggg
    31381 cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc ggcaggaatg
    31441 ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga agagactcta
    31501 tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt ttcagcgagg
    31561 gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca acaactggtg
    31621 gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac cgtttacgca
    31681 tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt
    31741 gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag gtacatcctt
    31801 cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa cccgtgttta
    31861 ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg cgtttgtttg
    31921 gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc
    31981 tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg atcattacaa
    32041 tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt
    32101 gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat ttacttggat
    32161 tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt tttatgaagc
    32221 aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa tcacattgat
    32281 tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac gttttgatta
    32341 tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa
    32401 accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt tcgttacggc
    32461 accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa
    32521 aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg ttgaccaagc
    32581 gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa aattagaact
    32641 aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag aactttatcc
    32701 aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac cgtacattga
    32761 aaaattaaag gaacaagatt tgttgaaaga cactgttctg attggactag gaacgaatgg
    32821 ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc gaaaagtata
    32881 ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga
    32941 acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct acagtaatga
    33001 ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga tgacacatta
    33061 cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca gcagtgagac
    33121 aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa
    33181 gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca aaacggttga
    33241 gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg cagcttctgc
    33301 ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa caggtcttct
    33361 tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta atcgtctaga
    33421 aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata cgtaaaaata
    33481 atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca tccatattgt
    33541 aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg attccttcaa
    33601 ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc cttttaggtg
    33661 caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga cgatggacca
    33721 tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact tcttctaacg
    33781 tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt ttatttgttg
    33841 gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta tgtcgaacga
    33901 cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt aaatggttaa
    33961 gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata gcgtcgatag
    34021 ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa
    34081 caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca tctaaagaaa
    34141 aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat tggtcgattc
    34201 gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt ctgtattata
    34261 ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta tgcatactat
    34321 gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg ggtagttcga
    34381 gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa
    34441 gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt atatctggcg
    34501 gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt caaaaatcca
    34561 gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga aaatccagga
    34621 ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag tttgatcatg
    34681 tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt gatttccaga
    34741 atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact tggggatctg
    34801 ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag
    34861 aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa
    34921 ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata aaatgataag
    34981 tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt tttcaatgaa
    35041 aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa aatagaagac
    35101 aggtgtcatg taatggatca aagtgttata ataattagaa ggacattttt tgaggtgatt
    35161 gaatgcgttt tttacataca gccgattggc atatcggaaa aaaactacaa ggttatgact
    35221 tgattgaaga ccagcgtcat gtactatctt ctatactaga tatcgcgaaa gaagaaaaag
    35281 tagaggcaat cgtgattgcc ggagacttat atgataggag tgtcccagct gttgatgccg
    35341 tagaattata caatgaattg atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg
    35401 ctatatcagg aaatcacgat agcagtacga gactttcagc gggtacgcca tggttcagtt
    35461 attcgaacta ttttttgcga acacagctag cagaagcatt tgaaccagtc gagtttaaaa
    35521 acgtacaatt tttctttctt ccttactttg aaccaattgc agcgagatta tattttgaag
    35581 atgacaatat ccgaacgatc aagcaagcgg tagccaaggt gatagataaa atgcaggagc
    35641 tatttgattc agaaaaaaaa caagtattga ttagccattt ttttgtaatg ggaagtgtaa
    35701 aatctgattc ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct ggtgaactac
    35761 ttacaccgtt tgattatgta gcactaggac atttgcataa tcaagctgct ttgagacagg
    35821 ataatgctcg ctatagcggt tccccattga aattctcttt atctgagatc aaccaaaaaa
    35881 aaggcgtatg gatcgttgac ctgacaacag gcctggagtt gaattttagg gagatcgaac
    35941 ccctgcatga tattgaagaa ataacagcga gtttcaaaga attgctggat cctgcctttt
    36001 atcagtccgt caatcgggag aattacatct atatctatct cactgataga acgatcatcc
    36061 cgaatatgat gaaccagctt cggcaaatat atcctcgtat tttggggatt gaacgggcaa
    36121 acggtcgagc agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa
    36181 aattagcaga agcatttttt agtgaaatga cggaagagca gctaactgca aaccaagaaa
    36241 aatggctcgg agaaacgttg caagaaatca tggaaatgga atgatcaaga atgaaaccga
    36301 aaaaattaca acttaaaaat tttggtcctt ttatcaatga gaccgtagat ttttcacgtt
    36361 tgacagaagc accgcttttt ttgatcagcg ggaagacagg agctggcaaa acgacgattt
    36421 ttgatgggat caccttcgca ctttttggtg aaacttccgg aagacttcgc tctggaaaag
    36481 agatgcgttc tttgtttgct acaccagacg aagaaacaag cgtgactttt tcttttgaac
    36541 atcagcagat gagctatgaa atcgaacgaa aaccagaaca agttttagca aaacgaaaag
    36601 gaaacggaac aaaaaaacag gcagccaaag taacgttgac catttttgat gggaaaggaa
    36661 aagaaataaa acaaatcaca aaacgtacag aagcagatca gttgatcaga gaactgatga
    36721 atctcgatgc gaaacagttt tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt
    36781 ttttggtttc atcaagcagt gataaagaga ctgttttacg tcatttattc ggtactcaat
    36841 ttttccaaca gttcaatgag cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg
    36901 atcatttaga gcaagagctg catcttctgc aaaaaaggtt tgttttagtc aaagaagagg
    36961 aaccagcagc agggtttgaa gccgttttag cccaatggac aaaccatcag gcaatactcg
    37021 aaaaacaaat cacggaagaa aaagaagctc taacggaact tcaggaaaaa caaaagcaac
    37081 tagaaaattc ctactatcgt tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat
    37141 taataaacaa gcaaaaggaa ttagctgctc aaaaagacga aatagaggaa aagaaacgat
    37201 ggattgggta ttatgaattt acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa
    37261 taaaagttga aaaaatagaa ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga
    37321 tgactacgca gaattatgaa gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa
    37381 caaaaatcaa agaagctgct caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg
    37441 aatccttacg tagtaaaaaa caagacgcag aacaattaac aatttcattg gataaagaag
    37501 aattgaatgc cgagaagctg tatcaaaatc tcgctatcta tcaggaaaga actcgaattc
    37561 ttcaagaaaa tttactggat caggaaaaac tgcaagaaga tcgtcttgct tttggacagt
    37621 taaaatatct agatgaaaag cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt
    37681 cgctcgcaca gataagagaa gaactcaaaa aagcaactga agaaataaca aaaaatagtc
    37741 agctattgaa agaacagaca gaacaagcag aacagctgaa aagccaatgg gcaaagcagc
    37801 aaattgctcg tcttcagctt tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa
    37861 cggaacaccc catacaacaa ttaaaacacg aacagccaaa taaggaagat atcttaaaag
    37921 tagaacaaca actggcagaa gctgagtctg ctgtagaaag aactcagcaa atcattgcca
    37981 aaagtgaagc acatgaaaat aatcttcaac aacaattatc agatttagaa caaaaaatca
    38041 tggcaacgaa tatacaattg gctgaacaag aagaacactt gcaacaggct ttgaaaaagg
    38101 catttcctga gataacagaa caaaaaacaa aaacaactct tgtagtaatc gaacaatctt
    38161 tagatagacg aaaaagcaag atggccaatg aaagaataga gctggaagaa ataagacaac
    38221 aagcagaaaa gctcgaacaa caattagctg tctacaaaca gcagcagaca gaaaaaaata
    38281 atcagctcca gcaactgcaa ggagaaatca aaagtttaga acaacaattg caagatacgg
    38341 atagtaactg tttagaagag gagatacgac gacttgaaaa acagttagaa gaagactcga
    38401 atcaactagc tgttgagcag aaagcaggag tgcaattgtt gcaagatctt catacgcttc
    38461 ggacacagct tgatcttcaa catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg
    38521 aacaagaaag aatagctaaa aagttagaaa gccagtcgta tttttcaaca attgaagaaa
    38581 tctgtgcatt tgcacaacag caagcacaat ataaagaaaa ccaacgtaca gttacttctt
    38641 atcatgaaga gcaattagtg acagctgacc ggctgcgtca attagaaaat atccagctaa
    38701 ctgagacatt cccagataca gaagagatca gccaacagtt agaagaaata agacgtatga
    38761 tcgaaataaa gcaagaaaag atatacggtt tacaagaaca aaaaattagt aatcaaaaaa
    38821 tctacgatga atgtctttcg atttatcaca caagccagac gaaattagat gagcttacgc
    38881 aattacagca gctttcgcaa acattcaacg gagaaaatcc taagaagact agtttggaac
    38941 ggtatgtgct tcaggtttat cttcaagaag tgttacaagt agcaaatgat catctccaac
    39001 gtttaacgaa aaatcgttat caatttgagc tagcggatac cattggcagt tatcgaggga
    39061 aaaccggatt agaaatcaat atctatgatg atcaggcagg tatgtctcga agtgcgcata
    39121 ccctgtctgg cggtgagagt tttattgcag cactttcttt agcattagct cttgccgatg
    39181 tgatccagac acaagctggt ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg
    39241 ggtcattaga tgaagaagct ttggaaatgg caatggaagc tttagaaacg atcgaaagtg
    39301 aaggaagaat gatcggcatc attagtcatg taagagaatt gaaagaacga gtattacaac
    39361 aaataagaat tgatacagaa ggtagcggac aaagcaaaat acgctatcaa cttggataaa
    39421 aagggagatt ggtaactatg aaatggagta ttccagaaaa agtcattgca cgtggacgtc
    39481 aatacttgca agaaggccga gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc
    39541 atgcagaagt cttgggcagt gaattgtata tggttgattt ggatggtaca gcaaaagaga
    39601 tagattactg ccaatgtccc tattgggagg aacataaata ttgtaagcat acagttgctg
    39661 tggaacttta tttacggtcg aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc
    39721 tagaaccggt aaaagcaaca agtgagggag aaatcattgc aaaaggtttt tctcgtctaa
    39781 aaacacaaaa caatcctcaa gcggtgcaac ctttgattat tgaataccaa gtagaaacga
    39841 ttgagactaa tcagtatcac ccagagcttt ctattttggc actttacttg cgtatcggtt
    39901 cattggcatc accgaaaaaa acatatatcg taaaaaacat ctatgatttt ttacaagctt
    39961 acacagaaaa agaaacttac actgttaata aacagtacca gtttcgtcta gataaacggg
    40021 cgtttcaaaa agaagatcaa aaaatactca cgcatcttgc aggaagtgct cagacgcatc
    40081 aattattagg tacgaacggc ttacaagtca aaggaaaact agataaaaga tatctgcttc
    40141 tcccagtaga acaagcccgc ttcctgttag agcagatgaa tcagactaca cgtttgcatc
    40201 tgcagataaa tgaacaaaac gatcaaggaa tacaattttc tgatcaaaac aaacctttat
    40261 cattcaaagt ggaaaaattt ggagaagatt atttgctgca agcattgaat gattttgatt
    40321 ttttctttat gcattatcag tggggagtta tccaagggac attttattct ctgacgaaat
    40381 atcaacaaac ggtttatcaa acttggaaac aattgattcg ccgattggag aaaccagaag
    40441 tagtgttcaa gaaaaaagaa ttaccgtctt tgtttaaaga gattatccct ctgctgtcag
    40501 agatcggcga cgtagctatt gcccctgcag tagaagagga agtagcagat atccccgtcg
    40561 aatttatttt ctttttccga aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct
    40621 atggtgaagt catctattcg actgacccga agcatgaggt ctcgacagaa caggcttttc
    40681 aggtgttacg tgacacagta gaagagaaac gagtacttga tctattcaga atgtatcgct
    40741 accagaaaaa tgaaactggt tatgaacgtc cattgccaag cggggaagaa ctatatgcct
    40801 ttttccgcac agaactatct gtcttccgtc agtttggtga agtcagactt ggaagaaaat
    40861 tgcgagagtt gtatttagat gcgcataaac atcaaccgaa aatcgaagta accgagaccg
    40921 gttcatggct cgatattcga ttcgatgtga cagggataga ggagcaagaa atcgatcatg
    40981 tcctgcaaag cttgctgaga aatgatgctt tttatacatt agaaaatggt caggttcttt
    41041 cttttgattc agaagaattt caacaaacaa gccaagtgtt gcaacaatta cgggaatcga
    41101 tacgtacaga agaaggaacg atccatgtac ctaaaaatca aggattgatc attcaaaatc
    41161 aattggaaaa aagcaatgct acttttagtg aatcgttcca aacgatggta caagatctca
    41221 ttcatccaga aagataccag gcacagcttc ccaaaggatt gaatgcaacg atgagagatt
    41281 atcaaaagca aggttttcga tggttgaaga tgttaggtca ttatcagttt ggcggtattt
    41341 tagccgatga aatgggactt gggaaaacac tacagacaat tgccttttta ctttcggaaa
    41401 aagaagaacg gaaaagcttc tctgctttga tcgtagcgcc tgcgagctta atttacaact
    41461 ggcaggcaga ggtgagaaaa ttcgcccctt ctttaagtat ccaagtaatc aatgggaata
    41521 aaaaagaacg tgaagaatta ctggctaaag acacagatat ccgagtgact tcttatgcca
    41581 gcttaagaca ggatctagct aattatcagt cgcaaaaaat tgattatttg attcttgatg
    41641 aggcacaaat ggtcaaaaat agcagtacga aaactgcaca agcattgaga gaattagctg
    41701 tacctcagcg gtttgcactg agtgggacac caatcgagaa taatttagaa gaattgtggt
    41761 cattgtttgc aacgattatg ccaggatttt tcccgactaa aacaaaattc agggaacttg
    41821 ccgcagaaga aattgcgcaa atgattcgac cgtttatttt acggcgagat aaacaaactg
    41881 tgttgaaaga cttgccggaa aaaacagaga tgaatctata ttctgcactt acagaagaac
    41941 aaaaaactgt ttatttagct tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt
    42001 ctgaagcatt caagaaaaac cggatcggga tacttgctgg tctgacaaga ctgcgacaaa
    42061 tttgctgcga tcctcgtctg tttattgaag attaccaagg cggttccggc aagctcgaac
    42121 aagtaaaaga tctactggta gctgccaaag agaacaaacg aagagttttg ctgttttctc
    42181 aatttacagg gatgctgact atcttgcaag aagagttagc cgagctaggg atctcgacat
    42241 tttatttaag aggcagtacg aaaccacaag atcggttaag tatggtagac gcatttaatg
    42301 ctggcgaaaa agatgttttt ctgatttcct taaaggctgg gggaactgga ttgaatctca
    42361 cgggagcaga tacggtcatc ttgtatgacc tttggtggaa tcctgccatt gaagaacaag
    42421 ctgctggacg tgcacatcgg atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct
    42481 ctgaaggaac gattgaggaa cgcatggatt cactccagca agaaaaacgc gaactgttcc
    42541 aaaaagtgat ccaaggtaat gaagaacagt tgacgaaact gactgaagaa gacatccgca
    42601 tgattttaag tgtaggagaa atcgaggagt aagagactcg aaaaagcgtt cagtaatggg
    42661 taagaaagcc tttctaaaga agcgattgtt tatttgattt aaaaaatagc aaggaggctg
    42721 ggattccatt ttatcccagc ttccttgcta tcattttttt atttctattt ttaaaggtta
    42781 tacaaagatt gtcttcattg tctctaacag ctgatcaaaa gaagaaatct cgatatcgca
    42841 aattggagtc tgttcaattt tgcgttctcg atggttgaac caaagcgctt gccagttagc
    42901 actttttgca cctagaacat cattgtcgta attatcgcca acgtataagg tctcttctgg
    42961 aagcatatga aattctttct cagccagctg aaaaatctct ttttctggtt tttggaaacc
    43021 tgttgcttga gaaatgagca tattgcggga aggaatccaa ttgttcaatt gaagttgctt
    43081 cagttttttt gtctgatggt cagtcggccc atttgtgata attcctaatg ggacattctt
    43141 ttccttcaag aaatcgaggg tttttttgac ctcctcatgc aagcagatat tatcaagttc
    43201 ttcctcgtaa attttttgaa ataagaggcc atcttcttcc gtaatatgag ggtagtctaa
    43261 atcttttagt gattggctga tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc
    43321 cattactttt gggaagtttt catcactatg atggcggaac cggatatata gtggatgcat
    43381 atctgcatct gaaacaagcg gaacgactct tttgaccgcg tttcgaaatg gctgctgctg
    43441 atcatacatc gtatcatcaa catcaaacac aactgattta atcaaaattt tacacgcctt
    43501 tcggttttca ttgatttttt cacaaatcct tgactattgt aacagaaaaa attgtctaaa
    43561 aaagctgata aataaaggtt tttcttacta tatttctatt tccttgacag aataaggttt
    43621 ttatatttca gtttttttca ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa
    43681 ttaatcatta tggtagaagt cgattgattt attttaatgc aatctctgtg gaattttcga
    43741 aagatttatt tttttaatac agtctcaata taactattca aacataaaat aacgttattt
    43801 gatttattt
//
LOCUS       DAZUKR010000008        43423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000008 DAZUKR010000000
VERSION     DAZUKR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.008"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..2389)
                     /locus_tag="ACG89C_000423"
     CDS             complement(8..2389)
                     /locus_tag="ACG89C_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4899005.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACG89C_000424"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACG89C_000424"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS4899006.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="ACG89C_000425"
     CDS             3090..3629
                     /locus_tag="ACG89C_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS4899007.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="ACG89C_000426"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="ACG89C_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS4899008.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="ACG89C_000427"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG89C_000427"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="ACG89C_000428"
     CDS             4736..5902
                     /locus_tag="ACG89C_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS4899009.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="ACG89C_000429"
     CDS             5948..7444
                     /locus_tag="ACG89C_000429"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS4899010.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="ACG89C_000430"
     CDS             7525..8208
                     /locus_tag="ACG89C_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4899011.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="ACG89C_000431"
     CDS             8853..10043
                     /locus_tag="ACG89C_000431"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4899012.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="ACG89C_000432"
     CDS             10145..11098
                     /locus_tag="ACG89C_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4899013.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="ACG89C_000433"
     CDS             11167..12516
                     /locus_tag="ACG89C_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4899014.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="ACG89C_000434"
     CDS             complement(12699..13022)
                     /locus_tag="ACG89C_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899015.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="ACG89C_000435"
     CDS             13151..14314
                     /locus_tag="ACG89C_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4899016.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="ACG89C_000436"
     CDS             14384..15733
                     /locus_tag="ACG89C_000436"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4899017.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="ACG89C_000437"
     CDS             complement(15778..16209)
                     /locus_tag="ACG89C_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4899018.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="ACG89C_000438"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="ACG89C_000438"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4899019.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="ACG89C_000439"
     CDS             19439..20035
                     /locus_tag="ACG89C_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4899020.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="ACG89C_000440"
     CDS             20157..21305
                     /locus_tag="ACG89C_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4899021.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="ACG89C_000441"
     CDS             complement(21343..21756)
                     /locus_tag="ACG89C_000441"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS4899022.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="ACG89C_000442"
     CDS             21835..22266
                     /locus_tag="ACG89C_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS4899023.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="ACG89C_000443"
     CDS             complement(22365..22598)
                     /locus_tag="ACG89C_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4899024.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="ACG89C_000444"
     CDS             22781..24451
                     /locus_tag="ACG89C_000444"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS4899025.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="ACG89C_000445"
     CDS             24598..24984
                     /locus_tag="ACG89C_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899026.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="ACG89C_000446"
     CDS             24971..25669
                     /locus_tag="ACG89C_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS4899027.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /gene="lspA"
                     /locus_tag="ACG89C_000447"
     CDS             25674..26138
                     /gene="lspA"
                     /locus_tag="ACG89C_000447"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4899028.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="ACG89C_000448"
     CDS             26135..27046
                     /locus_tag="ACG89C_000448"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4899029.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="ACG89C_000449"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="ACG89C_000449"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS4899030.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="ACG89C_000450"
     CDS             27834..29117
                     /locus_tag="ACG89C_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS4899031.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="ACG89C_000451"
     CDS             29130..30056
                     /locus_tag="ACG89C_000451"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS4899032.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="ACG89C_000452"
     CDS             30075..31355
                     /locus_tag="ACG89C_000452"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4899033.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /locus_tag="ACG89C_000453"
     CDS             31360..32436
                     /locus_tag="ACG89C_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS4899034.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="ACG89C_000454"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="ACG89C_000454"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4899035.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="ACG89C_000455"
     CDS             35680..36456
                     /locus_tag="ACG89C_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS4899036.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="ACG89C_000456"
     CDS             36453..37385
                     /locus_tag="ACG89C_000456"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS4899037.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="ACG89C_000457"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="ACG89C_000457"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4899038.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38731
                     /gene="pyrE"
                     /locus_tag="ACG89C_000458"
                     /pseudo
     CDS             38105..38731
                     /gene="pyrE"
                     /locus_tag="ACG89C_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            38758..39036
                     /locus_tag="ACG89C_000459"
     CDS             38758..39036
                     /locus_tag="ACG89C_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899039.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39074..39715
                     /locus_tag="ACG89C_000460"
     CDS             39074..39715
                     /locus_tag="ACG89C_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS4899040.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39758..40450
                     /locus_tag="ACG89C_000461"
     CDS             39758..40450
                     /locus_tag="ACG89C_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS4899041.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40447..41349
                     /locus_tag="ACG89C_000462"
     CDS             40447..41349
                     /locus_tag="ACG89C_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4899042.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="ACG89C_000463"
     CDS             complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="ACG89C_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS4899043.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13895 a         7673 c         9448 g        12407 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    38941 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39001 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39061 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39121 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39181 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39241 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39301 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39361 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39421 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39481 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39541 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39601 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39661 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39721 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    39781 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    39841 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    39901 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    39961 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40021 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40081 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40141 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40201 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40261 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40321 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40381 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40441 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40501 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40561 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40621 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40681 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40741 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    40801 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    40861 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    40921 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    40981 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41041 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41101 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41161 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41221 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41281 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41341 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41401 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41461 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41521 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41581 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41641 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41701 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    41761 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    41821 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    41881 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    41941 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42001 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42061 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42121 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42181 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42241 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42301 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42361 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42421 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42481 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42541 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42601 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42661 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42721 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    42781 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    42841 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    42901 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    42961 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43021 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43081 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43141 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43201 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43261 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43321 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttaggtat cgccgtttgt
    43381 ataattaagc gagacaaata aaaaagccat ttatgttaca ctc
//
LOCUS       DAZUKR010000009        41931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000009 DAZUKR010000000
VERSION     DAZUKR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.009"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..899
                     /locus_tag="ACG89C_000464"
     CDS             18..899
                     /locus_tag="ACG89C_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4899044.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            912..2522
                     /locus_tag="ACG89C_000465"
     CDS             912..2522
                     /locus_tag="ACG89C_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899045.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2599..3258)
                     /locus_tag="ACG89C_000466"
     CDS             complement(2599..3258)
                     /locus_tag="ACG89C_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4899046.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3541..4206)
                     /locus_tag="ACG89C_000467"
     CDS             complement(3541..4206)
                     /locus_tag="ACG89C_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS4899047.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACG89C_000468"
     CDS             complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACG89C_000468"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4899048.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACG89C_000469"
     CDS             complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACG89C_000469"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4899049.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACG89C_000470"
     CDS             complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACG89C_000470"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4899050.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6520..6840
                     /locus_tag="ACG89C_000471"
     CDS             6520..6840
                     /locus_tag="ACG89C_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4899051.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6952..7809)
                     /locus_tag="ACG89C_000472"
     CDS             complement(6952..7809)
                     /locus_tag="ACG89C_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4899052.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7917..9220)
                     /locus_tag="ACG89C_000473"
                     /pseudo
     CDS             complement(7917..9220)
                     /locus_tag="ACG89C_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9217..9726)
                     /locus_tag="ACG89C_000474"
     CDS             complement(9217..9726)
                     /locus_tag="ACG89C_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899053.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9901..12036)
                     /locus_tag="ACG89C_000475"
     CDS             complement(9901..12036)
                     /locus_tag="ACG89C_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4899054.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12119..13774)
                     /locus_tag="ACG89C_000476"
     CDS             complement(12119..13774)
                     /locus_tag="ACG89C_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4899055.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13776..14090)
                     /locus_tag="ACG89C_000477"
     CDS             complement(13776..14090)
                     /locus_tag="ACG89C_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4899056.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14260..14520
                     /locus_tag="ACG89C_000478"
     CDS             14260..14520
                     /locus_tag="ACG89C_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS4899057.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14534..14848
                     /locus_tag="ACG89C_000479"
     CDS             14534..14848
                     /locus_tag="ACG89C_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS4899058.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15578..16132)
                     /locus_tag="ACG89C_000480"
     CDS             complement(15578..16132)
                     /locus_tag="ACG89C_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4899059.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16258..18186)
                     /locus_tag="ACG89C_000481"
     CDS             complement(16258..18186)
                     /locus_tag="ACG89C_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4899060.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18492..19208)
                     /locus_tag="ACG89C_000482"
     CDS             complement(18492..19208)
                     /locus_tag="ACG89C_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4899061.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19209..21032)
                     /gene="glpO"
                     /locus_tag="ACG89C_000483"
     CDS             complement(19209..21032)
                     /gene="glpO"
                     /locus_tag="ACG89C_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS4899062.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21046..22542)
                     /gene="glpK"
                     /locus_tag="ACG89C_000484"
     CDS             complement(21046..22542)
                     /gene="glpK"
                     /locus_tag="ACG89C_000484"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4899063.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22678..24138)
                     /locus_tag="ACG89C_000485"
     CDS             complement(22678..24138)
                     /locus_tag="ACG89C_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899064.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24128..25423)
                     /locus_tag="ACG89C_000486"
     CDS             complement(24128..25423)
                     /locus_tag="ACG89C_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4899065.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25755..26432)
                     /locus_tag="ACG89C_000487"
     CDS             complement(25755..26432)
                     /locus_tag="ACG89C_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS4899066.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26462..27553)
                     /locus_tag="ACG89C_000488"
     CDS             complement(26462..27553)
                     /locus_tag="ACG89C_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4899067.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27654..28088)
                     /locus_tag="ACG89C_000489"
     CDS             complement(27654..28088)
                     /locus_tag="ACG89C_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4899068.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28672..30129)
                     /locus_tag="ACG89C_000490"
     CDS             complement(28672..30129)
                     /locus_tag="ACG89C_000490"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4899069.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30126..32021)
                     /locus_tag="ACG89C_000491"
     CDS             complement(30126..32021)
                     /locus_tag="ACG89C_000491"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4899070.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32079..33854)
                     /locus_tag="ACG89C_000492"
     CDS             complement(32079..33854)
                     /locus_tag="ACG89C_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4899071.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33904..35400)
                     /locus_tag="ACG89C_000493"
     CDS             complement(33904..35400)
                     /locus_tag="ACG89C_000493"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4899072.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35594..37216)
                     /locus_tag="ACG89C_000494"
     CDS             complement(35594..37216)
                     /locus_tag="ACG89C_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4899073.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37665..38978)
                     /locus_tag="ACG89C_000495"
     CDS             complement(37665..38978)
                     /locus_tag="ACG89C_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4899074.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39223..39447)
                     /locus_tag="ACG89C_000496"
     CDS             complement(39223..39447)
                     /locus_tag="ACG89C_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899075.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39467..39889)
                     /locus_tag="ACG89C_000497"
     CDS             complement(39467..39889)
                     /locus_tag="ACG89C_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4899076.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40033..40743)
                     /locus_tag="ACG89C_000498"
     CDS             complement(40033..40743)
                     /locus_tag="ACG89C_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS4899077.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40761..41258)
                     /locus_tag="ACG89C_000499"
     CDS             complement(40761..41258)
                     /locus_tag="ACG89C_000499"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4899078.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41295..41747)
                     /locus_tag="ACG89C_000500"
     CDS             complement(41295..41747)
                     /locus_tag="ACG89C_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4899079.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
BASE COUNT        11764 a         8934 c         7179 g        14054 t
ORIGIN      
        1 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
       61 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      121 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
      181 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      241 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      301 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      361 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      421 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      481 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      541 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      601 gactagcgga taaagtcgtg atcatacgcg aaggaacagt cgtagaagaa ggaacactcg
      661 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      721 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      781 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
      841 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
      901 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
      961 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1021 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1081 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1141 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1201 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1261 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1321 gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag
     1381 cagattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1441 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1501 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1561 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1621 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1681 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1741 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1801 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     1861 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     1921 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc ttgatttgtc
     1981 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2041 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2101 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2161 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2221 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2281 ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2341 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2401 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2461 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2521 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2581 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2641 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2701 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2761 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
     2821 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     2881 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     2941 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3001 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3061 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3121 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3181 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3241 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3301 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3361 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3421 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3481 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3541 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3601 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3661 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3721 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3781 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     3841 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     3901 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     3961 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4021 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4081 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4141 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4201 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4261 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4321 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4381 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4441 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4501 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4561 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4621 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4681 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4741 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4801 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     4861 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     4921 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     4981 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5041 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5101 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5161 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5221 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5281 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5341 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5401 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5461 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5521 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5581 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5641 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atccgcttcc
     5701 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5761 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     5821 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     5881 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     5941 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6001 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6061 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6121 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6181 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6241 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6301 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6361 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6421 tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta gtgcttcttg
     6481 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6541 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6601 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6661 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6721 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6781 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     6841 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     6901 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     6961 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7021 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7081 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7141 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     7201 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     7261 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     7321 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     7381 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     7441 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     7501 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     7561 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     7621 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     7681 agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     7741 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     7801 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     7861 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     7921 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     7981 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     8041 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     8101 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     8161 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     8221 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     8281 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     8341 cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat cttcaacgtc
     8401 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     8461 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     8521 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     8581 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     8641 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     8701 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     8761 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     8821 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     8881 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     8941 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc ttcgatcaca
     9001 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     9061 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     9121 catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata gacaaacaag
     9181 tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca ccccattttc
     9241 tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga cagaataaaa
     9301 acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt cttcaagtaa
     9361 ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta cttttttgta
     9421 tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat
     9481 caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa aagcgtacaa
     9541 ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata taaaaggaag
     9601 aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta accagtaatt
     9661 accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc ttgttacttt
     9721 tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat acatttcatt
     9781 tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac atttctcatc
     9841 agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt tatctcttaa
     9901 ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg ccgtgtacat
     9961 ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga ttacatgatc
    10021 aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta atccatatga
    10081 atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg tgataactgc
    10141 cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca attcttcaga
    10201 ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata cgatgtcgat
    10261 cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc tagaacggta
    10321 actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac aacgcataat
    10381 atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa
    10441 gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat aatcttgtac
    10501 ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg
    10561 aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag cataagctga
    10621 gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc atgccatttc
    10681 ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg cataagctac
    10741 aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact gcctatcttc
    10801 tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca aaagatgatt
    10861 cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca atttaggatg
    10921 agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg tttttccatt
    10981 tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg attgttttag
    11041 attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat tttctcccgt
    11101 gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct gtgaaaccag
    11161 ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga cattcgaaat
    11221 atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg tcatcgtatc
    11281 tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt taccacttcc
    11341 tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg tgttttgttc
    11401 cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc ctaagacttc
    11461 taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca aacgatctct
    11521 ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt gacgaaccgt
    11581 ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg aacgccaatc
    11641 ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat aaaaggattc
    11701 ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac ttttcaataa
    11761 acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca tctgatcgat
    11821 ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa
    11881 attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta tttttgccgt
    11941 atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca cgttcttcag
    12001 atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct ttttttaaag
    12061 tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt aacgagtatc
    12121 atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa aatgcaccat
    12181 caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga tagctacgta
    12241 atccgctatg acagctgacg atatattctt gttggctgtc taattcattt aatcggtccc
    12301 gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc aattcttctg
    12361 ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat tcatgccatt
    12421 gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc atgtttactg
    12481 gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt aaatcgaaga
    12541 tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc acaccttttg
    12601 ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc aacttcagta
    12661 aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca tgaatgactt
    12721 caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat gcggcagtca
    12781 ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga gaaattccgg
    12841 aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga gaaataagtg
    12901 catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca taaatatcag
    12961 gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct aattcgattc
    13021 cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata actaaatcag
    13081 cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca aatccagcga
    13141 cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct ataaaagctg
    13201 ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc atgacttcta
    13261 aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca ccaacgatca
    13321 cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct ggtacatttc
    13381 taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca actggttttg
    13441 caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc tcatgtacga
    13501 cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga cgcacatcta
    13561 aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc cgttcactga
    13621 tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac ggtccttttt
    13681 caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca gcagacattc
    13741 cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac tgttcgatca
    13801 aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt ctttaattct
    13861 ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt cttttctttg
    13921 tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt tatatgcccg
    13981 ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa aagcttttcc
    14041 tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat tttctgcacc
    14101 tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt attaatatgt
    14161 caaccagaat gactcttcat ttttttcact actttttata tttgcataaa ataccgataa
    14221 ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga taataaaaat
    14281 atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa aatgatcgaa
    14341 gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc tagtattgat
    14401 cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa acctgatagt
    14461 aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat taaaaagtaa
    14521 aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac
    14581 agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg tcgtatctgt
    14641 aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct tagatttgca
    14701 aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt cagaaagttc
    14761 tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg aactgcaatc
    14821 ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag tgatcagctt
    14881 caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt
    14941 gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    15001 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    15061 ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa atagctcctc
    15121 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtttaaggaa
    15181 gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggattt
    15241 ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat accgtttaag
    15301 gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg gaaaaatagc
    15361 tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta
    15421 ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt caaaaacaac
    15481 ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg agtctattta
    15541 tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga aacatctttc
    15601 ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa
    15661 tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc atgccaacat
    15721 agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg atatttttag
    15781 atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat agaccaaaca
    15841 acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt
    15901 tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat ctccgagcaa
    15961 tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct gctatataaa
    16021 atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg aatatcgata
    16081 aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc attttatcgc
    16141 cctacctcct cagcactctt ttctttttat tttagcttac acggggaata aaaccaatga
    16201 tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt gagataatca
    16261 cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt cttttactag
    16321 tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac gtgttttata
    16381 atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac
    16441 cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata catcaatcgt
    16501 atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga caaatatccg
    16561 atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg taactgctgt
    16621 cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt aacgctctgc
    16681 ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag cttggctttc
    16741 tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat cgaaagccat
    16801 agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc cataatgggt
    16861 cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga tcgctagtgc
    16921 agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca acgtattcct
    16981 ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc
    17041 attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga cagcaagctc
    17101 tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca ataaaccgac
    17161 tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca agcctcgata
    17221 gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc
    17281 ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat tgatttctcg
    17341 gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga tcaataaatc
    17401 gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt gatattcttc
    17461 actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa tcacagttgg
    17521 ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat ctattttatt
    17581 tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa tcattgcttc
    17641 ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg actggatttc
    17701 tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact
    17761 gactttatta ttttttattt tctggtcaat caactcttct acatttatca aatcaggaag
    17821 atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa aaaactttgt
    17881 gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg gctgcagaat
    17941 caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca taaatggaac
    18001 atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac
    18061 agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat agtttccaat
    18121 tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa tcatttcgtt
    18181 gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa gggcctagag
    18241 ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat tagaatacaa
    18301 agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact ttgtttattg
    18361 gctattcgac ttactgatta gataccagct acactcacaa aacatacccc attcttctag
    18421 gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa actttagtat
    18481 gttttataga tttacaccat taatgaatag acacctaccg cgataattgc tccgatcatt
    18541 ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt agctattggc
    18601 aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc atatcctgtt
    18661 ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa tacgttcgtt
    18721 ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac aaatgtacca
    18781 attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt tgcaaacgtt
    18841 cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga aagccaaaca
    18901 agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac gacaagattc
    18961 caactaattg cccctgtcat cgccatagct agagagactg caggattcaa atgagctggg
    19021 cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc aaatgcgatg
    19081 acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc acacacacca
    19141 tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg agtcatatca
    19201 ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat caatgcttct
    19261 aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc agcaattgca
    19321 tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag atgatttgta
    19381 cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc ataacgtaac
    19441 cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc taattcaaag
    19501 atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc ttcctctaaa
    19561 ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt agttggatcg
    19621 aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc tattccgtat
    19681 tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt acggtaatcg
    19741 gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa agaactacct
    19801 ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt caacacgtct
    19861 tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc gacaacttta
    19921 ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga accagaatta
    19981 ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa tgtgatattt
    20041 gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc ttgtgtgact
    20101 ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta agtcttgttt
    20161 tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa gtatgtgggt
    20221 tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt tggacgcatt
    20281 ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca aggaccactc
    20341 gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag gtcacgagct
    20401 ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat tttacttact
    20461 agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg ggcatcattg
    20521 ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa aaacggttct
    20581 ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc tgtcacattt
    20641 gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa ggttgttgct
    20701 ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg tggggcaata
    20761 ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc aaatgtcttc
    20821 agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa
    20881 tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac tgccacaccg
    20941 gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg cattttttga
    21001 atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt caaatgcttg
    21061 ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct cttcctccat
    21121 ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg tatctttcca
    21181 ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc
    21241 tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat attcattgtt
    21301 tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt
    21361 atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa tgatatcttc
    21421 ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat cccaataagg
    21481 agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg tagagcgttt
    21541 cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc tcagccattg
    21601 aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct ttccatttat
    21661 gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt caccggtatt
    21721 catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt cgaatgccat
    21781 ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg cttcgcttcc
    21841 ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga cttttggtaa
    21901 catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta gctgatggat
    21961 attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt gtccatcggt
    22021 cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt tttcagctcg
    22081 ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg agaaataggc
    22141 atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat tttttaagtt
    22201 atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga ttggacgtcc
    22261 tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa tcccagcaat
    22321 ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact gtacagaatt
    22381 ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt gcgtgaactc
    22441 tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc gtgagctagt
    22501 tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc cctccattat
    22561 atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa gaaactgtca
    22621 aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat tttttaatca
    22681 attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt cgtaaggtga
    22741 cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa atgtgatcac
    22801 caaatcatat tctttacctt cttcataact tccaatctca ataccgccga ttcccctcag
    22861 ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt ctaaatcgat
    22921 tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat tggcatactg
    22981 gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt gctgcaccgc
    23041 tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt tctgctgctg
    23101 ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa accattcatt
    23161 gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca aacgacgagg
    23221 acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg ttggtaactt
    23281 tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg aaaaagaaac
    23341 aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg ttcgactaag
    23401 atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat agagtaattt
    23461 cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt tcgtaatccc
    23521 gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta atacacggtc
    23581 caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc
    23641 ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct cttctccgta
    23701 taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca attctttgat
    23761 ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa cgatggatac
    23821 ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct gcaataaaaa
    23881 caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct gatactcatt
    23941 tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat cttccaaata
    24001 ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga tagaggctgt
    24061 tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt ttctttctaa
    24121 taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata tctggcacac
    24181 ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc catccttctt
    24241 ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt tggagctgtg
    24301 ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg ctttgattta
    24361 ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa aaaatgctct
    24421 cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg ccgactggac
    24481 gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca tttcgtgctg
    24541 tacggacagc gttatttact agagaaacat acattttttc atttgttgga cgaaaagtta
    24601 cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag tagtcatcta
    24661 cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga ttgtcggggc
    24721 gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc gtctctacaa
    24781 tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt gaaagccctt
    24841 gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa cgaaaaagaa
    24901 ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac ccttcagcta
    24961 tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc atgatctttt
    25021 gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt gattctgcag
    25081 aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca aaatccaatt
    25141 tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc ttttctgtgt
    25201 aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa agttgttttt
    25261 cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat cctaacttcg
    25321 attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga taaatagccg
    25381 catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt cctctttctt
    25441 ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa ctcgctttca
    25501 accttatgat acatagcgat gcagcagaat caaccctctc catcataccg tgttctaggt
    25561 ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg aagagatgtt
    25621 tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc tgtccaaata
    25681 ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag ttagaataga
    25741 cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa ttgatgagca
    25801 gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc tgaaaaagct
    25861 gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa gaatggtact
    25921 tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac tgtacgtcca
    25981 gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac accgtgacaa
    26041 acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc aagatctatt
    26101 gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc tgcataatca
    26161 tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct gaaatcttca
    26221 tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg ttctaaaggt
    26281 acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac taatacatca
    26341 taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt ttcgtacttt
    26401 tctacatttg ttaatacgat caatactttc attttcccat cctctttcta taaaaattgc
    26461 gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa agctctttat
    26521 atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat aatttctctg
    26581 cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca tatttcaata
    26641 cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg atctttcctt
    26701 gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca gaacgagcta
    26761 ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca cgtaatcgtt
    26821 caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct tctggtgaga
    26881 aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag aaaaccgttg
    26941 ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat tgatcctctc
    27001 gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct ccatgcaact
    27061 cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat gcttggattg
    27121 tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga cgttttgctg
    27181 caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt gtcattcgtc
    27241 caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct ttcgctagct
    27301 cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc cagccttttc
    27361 cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta gaacgttgtt
    27421 tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca
    27481 taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta attgattgat
    27541 caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc gagaatcatc
    27601 tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa attttatttt
    27661 ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact tgcttcattt
    27721 cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac ttccttagca
    27781 aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct aactgttttg
    27841 acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag
    27901 agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat caataaaata
    27961 aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa tttattcaac
    28021 atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt ttcaattggc
    28081 ttttccattt catcccctcc attggcgtac atatcatagt atgattttta ttttttgtca
    28141 aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa gtagccatct
    28201 tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt
    28261 gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat
    28321 ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt tatatcagag
    28381 gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    28441 tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg tttattgaga
    28501 ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa acagcttctc
    28561 atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac tgtttattcg
    28621 taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt attataggat
    28681 ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct ttaaccgttt
    28741 ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac gttttgacat
    28801 ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac caaataaatc
    28861 tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat agtcaatgcg
    28921 gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga gtccgttttc
    28981 agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa ttctcaaacc
    29041 cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt taattcctcc
    29101 agttattaag tttccttgga ccccttccca attttctgga tgagcactga ctgcactaga
    29161 catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt cttcatcacc
    29221 tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat acgctggata
    29281 atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct caattgtttt
    29341 ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga tcatacaacc
    29401 gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac ttgctataaa
    29461 ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt cttcaattac
    29521 tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag tcatccaata
    29581 ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat
    29641 aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca tttcataatg
    29701 ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca cttgatcata
    29761 aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca
    29821 tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga tgtcttcttt
    29881 aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc tgttgggatc
    29941 ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc atttttcttt
    30001 aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt catctacatc
    30061 ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc cttctggaaa
    30121 ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt tttcttatga
    30181 ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt ggtcggatca
    30241 tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc tttacgtact
    30301 ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac tgtatcaata
    30361 ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc atgtttcgtt
    30421 ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga tttttccgtc cgttggaatg
    30481 atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac ttcagataaa
    30541 tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga ttcttcttcg
    30601 atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat cgctaagaaa
    30661 taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga caaaatactt
    30721 cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc aaatatagct
    30781 ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc ataaacagca
    30841 ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact ttttactttc
    30901 ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc tacggcagtc
    30961 atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt cattactggt
    31021 acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc gataacagca
    31081 ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat cagctgactt
    31141 gcataatctg caattggacc tacaactaat aaagtaattg ggaccatcac gatgatacaa
    31201 atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca
    31261 atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt ataccttgct
    31321 agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc actgacaatc
    31381 gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt attgaacttt
    31441 tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc agctgctgca
    31501 ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt acataaggcg
    31561 agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc aaaaacagcg
    31621 gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct ctttccacta
    31681 gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc atttactgca
    31741 tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac accttctagt
    31801 tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg taaccttgtt
    31861 gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac aatatctttc
    31921 gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt agcaatcctt
    31981 cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt accccttata
    32041 atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa atcactcact
    32101 gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc
    32161 ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt gatttttgtt
    32221 tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg aagcataact
    32281 aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg gatttttatt
    32341 tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc ttcaactatg
    32401 gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc tagaacacta
    32461 ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt caaaattgca
    32521 tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc tatccggcat
    32581 tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt caaaatacta
    32641 attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt caacgcttcg
    32701 gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg aaaatgtatt
    32761 gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat cttttgtaca
    32821 aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt gatatgggtt
    32881 agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa acgaataatc
    32941 gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc attaatcatc
    33001 aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt ggaaaaagaa
    33061 tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat aaataatgta
    33121 tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac gagataccga
    33181 gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag aacctctttt
    33241 aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg ttgaggcaac
    33301 cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct ttttgaattt
    33361 tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa tttaggattg
    33421 cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt tgaaagaaag
    33481 aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa gtacgtcaca
    33541 atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac ctcttgtatg
    33601 atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg tttatttgac
    33661 tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt ttggatcgtt
    33721 ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg attcttattt
    33781 cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt acacctcctc
    33841 tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc agaggtttaa
    33901 aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga taatttgttt
    33961 ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat cattttgtct
    34021 atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg atacgatatc
    34081 gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg cctcagcaat
    34141 ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc gattcaattc
    34201 atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta attcataacg
    34261 gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc aactccaagg
    34321 ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc cagtatctac
    34381 gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg caatataatc
    34441 tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg cacagtcttc
    34501 ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga catcggagaa
    34561 aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat cacagtcata
    34621 tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat ttggatcgat
    34681 tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac aggcttgata
    34741 aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag ggaatgtaac
    34801 caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa aggtcatcca
    34861 atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc
    34921 gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg gggtttcata
    34981 atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga ttagtctgtc
    35041 ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa atattcgtgc
    35101 ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac gaatatcctc
    35161 ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg attcgtctaa
    35221 agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct ggttcatgat
    35281 cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg caccttcaca
    35341 ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt cctgatccat
    35401 aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat tcatcaaaac
    35461 aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac tcttcatttt
    35521 taatatttat atataacaaa tacataagac gatccccgaa agaaaacact cgttaaccat
    35581 ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt aaatcaactt
    35641 tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa gcttcacgtg
    35701 gaacagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat tgctggtctt
    35761 catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat tcatagtctc
    35821 cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac atataaaaaa
    35881 cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt ggattgactc
    35941 gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat tgcataggcg
    36001 tcctagcatt gtctcttcct ttcgcattga ttgattccaa gatttcctct tttgtaaatc
    36061 cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct tccgcgatat
    36121 catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa ggggtgcctt
    36181 tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac acatcgtcat
    36241 ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa ctattccatc
    36301 cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca gagatactca
    36361 agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg tgttcgaatt
    36421 ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg atttcggtag
    36481 ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt gcttgattca
    36541 tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta tccggttctt
    36601 tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg atccagaaat
    36661 tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa tcaggttgac
    36721 gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc caagcagaac
    36781 cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca cgccaaatat
    36841 aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac ggatgttcat
    36901 cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc tttgcttctt
    36961 caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct tgataattac
    37021 tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc caaattgcat
    37081 caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa tctccaatac
    37141 catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact gaatggtgcc
    37201 accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt tgcttaatca
    37261 tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt gcaccttgtt
    37321 atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa
    37381 ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac ggtgttcact
    37441 cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat gaggaactat tttttcgttt
    37501 ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa cggtgttcac
    37561 tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt
    37621 tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt cacattcaat
    37681 atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga tacttctttt
    37741 cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc aatcccatct
    37801 gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat cgcaatctct
    37861 aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac cataatcaat
    37921 gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa caagcctaac
    37981 aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac ggcgatacca
    38041 gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc cgcaagcata
    38101 gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc tttcatgaaa
    38161 cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt cacaagcaag
    38221 aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg ttttaatgca
    38281 actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg cattggaatc
    38341 aaacctgtga ccagacctaa aatcgttgta gctgctatcc cgatgaagat gccacctggt
    38401 acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa ccaagtagaa
    38461 ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat gatcccacct
    38521 tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc atatttcaaa
    38581 tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat caaaataaaa
    38641 atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc cattcctaaa
    38701 caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc aattggataa
    38761 cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt cgcagtaaac
    38821 actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa taagatataa
    38881 gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt ggtgttcaat
    38941 ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc taaatccgaa
    39001 cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg ttttatcata
    39061 tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc
    39121 atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt acaagaattt
    39181 ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt gtgatttgat
    39241 tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag gattcagatt
    39301 atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct tcttttttcc
    39361 tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat acttcaaata
    39421 accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa tcagacgact
    39481 cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa gtcaagatgc
    39541 cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt tcaaattcat
    39601 ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa
    39661 atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca atactcttaa
    39721 attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct acaaagaatc
    39781 ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt atcatcattt
    39841 gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt atatcacgaa
    39901 agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt ttctaggatt
    39961 gctttcctcc acaaactttt tttacaaaaa aaaaaaacgg caatgaatgc cgctcttctt
    40021 atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt gtctaacact
    40081 agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct gtgcgccacg
    40141 tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg tttcttccgt
    40201 cagcattgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc cttgttcatc
    40261 atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa
    40321 tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt cgatatcttc
    40381 tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta caagcggatt
    40441 gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa tagaagaacg
    40501 tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt
    40561 ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct cattcaataa
    40621 ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat ttacggataa
    40681 gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta ttactttttt
    40741 catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc tgtttgggga
    40801 tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt acgataaaaa
    40861 gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct
    40921 tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg tttaaatttt
    40981 tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc agattgactc
    41041 gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac taggaaaaaa
    41101 tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc ttgtgtcaag
    41161 taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa tgtttcgata
    41221 ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc gtatttattc
    41281 acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt ccaatcttcg
    41341 ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc ttcgtcagaa
    41401 agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa aggatagatt
    41461 tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt atcttccaaa
    41521 cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt tgtacgatct
    41581 actttgatca tttctgctag tttttcttgg atgattccag gattttcgca aatacgtact
    41641 aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt gctaatagaa
    41701 tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc atcccacctt
    41761 tattttgttt gtaagactag tttacctctt tttgttgcaa atgcaactat tttcaataaa
    41821 aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt tacgaaaaag
    41881 ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc t
//
LOCUS       DAZUKR010000010        41333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000010 DAZUKR010000000
VERSION     DAZUKR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.010"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..755
                     /locus_tag="ACG89C_000501"
     CDS             78..755
                     /locus_tag="ACG89C_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4899080.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            758..1885
                     /locus_tag="ACG89C_000502"
     CDS             758..1885
                     /locus_tag="ACG89C_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899081.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2085..2525)
                     /locus_tag="ACG89C_000503"
     CDS             complement(2085..2525)
                     /locus_tag="ACG89C_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4899082.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(2536..4569)
                     /locus_tag="ACG89C_000504"
     CDS             complement(2536..4569)
                     /locus_tag="ACG89C_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS4899083.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4571..6274)
                     /locus_tag="ACG89C_000505"
     CDS             complement(4571..6274)
                     /locus_tag="ACG89C_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4899084.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6368..7318)
                     /gene="manA"
                     /locus_tag="ACG89C_000506"
     CDS             complement(6368..7318)
                     /gene="manA"
                     /locus_tag="ACG89C_000506"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS4899085.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(7357..8382)
                     /locus_tag="ACG89C_000507"
     CDS             complement(7357..8382)
                     /locus_tag="ACG89C_000507"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4899086.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8591..9460)
                     /locus_tag="ACG89C_000508"
     CDS             complement(8591..9460)
                     /locus_tag="ACG89C_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4899087.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(9919..11811)
                     /locus_tag="ACG89C_000509"
     CDS             complement(9919..11811)
                     /locus_tag="ACG89C_000509"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899088.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(11808..13535)
                     /locus_tag="ACG89C_000510"
     CDS             complement(11808..13535)
                     /locus_tag="ACG89C_000510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899089.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13551..14201)
                     /locus_tag="ACG89C_000511"
     CDS             complement(13551..14201)
                     /locus_tag="ACG89C_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS4899090.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14405..14701
                     /locus_tag="ACG89C_000512"
     CDS             14405..14701
                     /locus_tag="ACG89C_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4899091.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            14873..15493
                     /locus_tag="ACG89C_000513"
     CDS             14873..15493
                     /locus_tag="ACG89C_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4899092.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15585..16751)
                     /locus_tag="ACG89C_000514"
     CDS             complement(15585..16751)
                     /locus_tag="ACG89C_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4899093.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16763..17116)
                     /locus_tag="ACG89C_000515"
     CDS             complement(16763..17116)
                     /locus_tag="ACG89C_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4899094.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17244..18266
                     /locus_tag="ACG89C_000516"
     CDS             17244..18266
                     /locus_tag="ACG89C_000516"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS4899095.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            18285..18446
                     /locus_tag="ACG89C_000517"
     CDS             18285..18446
                     /locus_tag="ACG89C_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899096.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(18705..19139)
                     /locus_tag="ACG89C_000518"
     CDS             complement(18705..19139)
                     /locus_tag="ACG89C_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899097.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19249..21108)
                     /locus_tag="ACG89C_000519"
     CDS             complement(19249..21108)
                     /locus_tag="ACG89C_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4899098.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            21109..21249
                     /locus_tag="ACG89C_000520"
     CDS             21109..21249
                     /locus_tag="ACG89C_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS4899099.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(21226..22386)
                     /locus_tag="ACG89C_000521"
     CDS             complement(21226..22386)
                     /locus_tag="ACG89C_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4899100.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22693..24117)
                     /gene="araA"
                     /locus_tag="ACG89C_000522"
     CDS             complement(22693..24117)
                     /gene="araA"
                     /locus_tag="ACG89C_000522"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS4899101.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24133..24831)
                     /locus_tag="ACG89C_000523"
     CDS             complement(24133..24831)
                     /locus_tag="ACG89C_000523"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4899102.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(24788..26449)
                     /locus_tag="ACG89C_000524"
     CDS             complement(24788..26449)
                     /locus_tag="ACG89C_000524"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4899103.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26667..28067
                     /locus_tag="ACG89C_000525"
     CDS             26667..28067
                     /locus_tag="ACG89C_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS4899104.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28319..29398)
                     /locus_tag="ACG89C_000526"
     CDS             complement(28319..29398)
                     /locus_tag="ACG89C_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4899105.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29484..30443)
                     /locus_tag="ACG89C_000527"
     CDS             complement(29484..30443)
                     /locus_tag="ACG89C_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4899106.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30455..31966)
                     /locus_tag="ACG89C_000528"
     CDS             complement(30455..31966)
                     /locus_tag="ACG89C_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS4899107.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(31988..32551)
                     /locus_tag="ACG89C_000529"
     CDS             complement(31988..32551)
                     /locus_tag="ACG89C_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4899108.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32557..33420)
                     /locus_tag="ACG89C_000530"
     CDS             complement(32557..33420)
                     /locus_tag="ACG89C_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899109.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33434..34330)
                     /locus_tag="ACG89C_000531"
     CDS             complement(33434..34330)
                     /locus_tag="ACG89C_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899110.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34505..35794)
                     /locus_tag="ACG89C_000532"
     CDS             complement(34505..35794)
                     /locus_tag="ACG89C_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4899111.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35766..37283)
                     /locus_tag="ACG89C_000533"
     CDS             complement(35766..37283)
                     /locus_tag="ACG89C_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS4899112.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            37481..38371
                     /locus_tag="ACG89C_000534"
     CDS             37481..38371
                     /locus_tag="ACG89C_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4899113.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(38444..39268)
                     /gene="nadE"
                     /locus_tag="ACG89C_000535"
     CDS             complement(38444..39268)
                     /gene="nadE"
                     /locus_tag="ACG89C_000535"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4899114.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(39280..40752)
                     /locus_tag="ACG89C_000536"
     CDS             complement(39280..40752)
                     /locus_tag="ACG89C_000536"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4899115.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12006 a         8614 c         7146 g        13567 t
ORIGIN      
        1 caataacttt tgttagcatt taatttagat gttttccatt ctattatgtt attaaatttt
       61 atattggagg cctatctatg acattactga tcaaaaacat gaacatgaaa cgtggtaaaa
      121 aacgtgtgat tcgacaattg aatcttttag tagaatcagg agaagtagct gctttgattg
      181 ccccgaacgg atttggcaaa acaactttgc tgaatggcat tgcccaactg ctgccaacaa
      241 cttatgatca attgtccctt cacaacatct ctcctactgc ttatacagat tatcgccggt
      301 ctttcttttt tctagaatca acgaatcagt tatccgacta tctatcggct gttgattatt
      361 tgactttgat caaatactat tggcagagca aagtagaatg ccaaactgtt ttccaacgat
      421 tgaagatgac tgagtacctt actttgccga ttggaaaaat gtctctagga atgaaacaac
      481 atgtcttgct tgctgcttac ttgatcagtg attcatctgt catgttattc gatgaaccgt
      541 tgaacggatt agacccagga agtattgata tcttgaatag cctcatcaga caatggcaga
      601 aagaggggaa gacgattctt ctttcttccc acgctatcgc gaatatccaa tcgatttgtt
      661 ctaaagcatg ttttctaaaa aatggagaaa tcgtcacaga atcctcaaat atggaagaga
      721 ttttagctat ttatcgtaaa ttatatgagg tgtaactatg tctttactta catttgaacg
      781 caatctctta ctaaaaaaca aagtaaactt tttatttcct ctattgttgg tcgtgctctt
      841 tgcattccca cttttttttg atcataaact tgcttataca gaattcgatg aactgaatca
      901 taactatgaa gagatgcaac gtttgattga aacgttgaaa gaagatgaga atgagaaaga
      961 atttgtggaa agtttagaga aaagcaacaa actgatcgaa gcaatcttac atgcaaaaaa
     1021 cacagggaat gtccagcaaa tggtcgaagc aacttatcat tatgaaaaag atatactgga
     1081 tcgactgatt tctggtcaaa gacaaggaat tccaatcatt gaacaacaaa aacgtgtcga
     1141 gttgctccgt tatatgaaag aacatcagat ccaaagatat tctatttttg acttgccagc
     1201 gcatctccct ttagcaaatt actacgaaaa tattttttca ggaatgattt ctagtttttt
     1261 gattttatgt ataaccgcct tgtttctttc ttccatcatc tcttatgaaa aaagaaagca
     1321 ggtaatctct ctagtcaatc tattaccgga tagtatggta aagaaacata gcatccgttt
     1381 taccatttat tatggtgcag ctatgctaag tctcgtgatg ccatttttga tcgtctctat
     1441 actagtaata atcaaaaatg gtttgggaga cttccgttat cctgtaggaa cgatcattgg
     1501 tcaggagata cgaatccttc cgatgtatga gtatcttttc cagagctttc tgttcctgtt
     1561 gttgtgggtg ctctttttaa gtacaatcag ttttcttcta tctgctttat ttgaacattc
     1621 tctagtcaat cttttaggta cacttctttg tcttttctta gcagagtatc gcttgttttc
     1681 ttcgattgga tggatagaat caatcagtca ttacttgcca acttcttatg ttgattttca
     1741 aaatgtgatc ataggaggag atattttctc gccactagct tcagaacagg ttactttcat
     1801 gaatggtatc ttgacacttg gtatatggag tatcgtttta ttattcatag gtatgggcac
     1861 tatctatata aaaaagagct attaaaacac aaaaataata ggggcggtga caaaaacctt
     1921 gtcaccgtcc cctatttcaa ataaacggtt ttctagaata aatagttttc aaaagcttgc
     1981 atccgcagta ataagaacct cccaacaaaa atagctcctc ttatttttat cttggttgaa
     2041 gttattactt gatgcaaggc agcttttttt actgtgaaca ttggttatat atgtccatgt
     2101 tcaaaaatca tttggacttc ttcaatggtt ttcgcatcac tgagcgccat tacttggtcg
     2161 atgtctgaac agaaaaaggc taaatcttgg agcaccgcta actgatgttc tttttttaaa
     2221 gccactacaa ataagagtgt cgcgattttt ggttcctctt ttgttccaaa gtccacacca
     2281 tcaggcactt ggactaaata gattccttct tctactactt cttcatcttt gtcagtccca
     2341 tgtggcaagg ctacgaaatt cccgatatag acactcgctt tttcttgtct ttcgatcatt
     2401 gattggatat atgccggttt gattccccct tgttgggtca tccactggcc gatctcggtc
     2461 aacgcttctt tccaattttg ataggattga tcgagcaata ttttttctgc tggtagctgt
     2521 atcatctttt caccttcatt tctttatttc ttcaatcagg attttcgcta acaactgata
     2581 aacgatttct ttatttcctg agcggaagat ctcggtgttc aagtcattca tgatgattgc
     2641 tccactgatc tttcctaaaa gaatcccgtc tttttcttgg attggcgaag gcgccagcat
     2701 aactaacata cgggacaaat cgactggctg tctatccatc ccttcaatgg tcaacggatg
     2761 tttcaaattg aaaatacaaa agatcggttt agaaatcaat gagctggatg tatggaataa
     2821 ccccatattt gtatgaggaa taccgatagg tgcttgttca tatcgcttaa gcagccggcg
     2881 atgaaccacc tcttcatccg ttactagtcc ttcaggaaaa taaggaaata agctgcgaat
     2941 cgtttcggaa atcgtctcag gattatccag ttcttggata aaaaaacgac cgagtaattg
     3001 attgatttga tccatgaaat gcattgtttc ctcataagta tcttctatca tccactcgtt
     3061 attgctgtta tacgtaattt tttgcctttt tttcttctca atatgcgaaa aagcttcttt
     3121 tagctgcttg acttcatctt ctagcagtag cggggagact aaaatatatt ttccaccgta
     3181 tcctggtaaa atagatgtag aaataacaac atcatattct tcttgtaaat cgattttttt
     3241 taaatcagca atccgataga aatgaatttg ttggatgaag ggaaaatacc ttttcaatcg
     3301 tagttccaac atgctagtag aagctaatcc acttggcgaa aatcctgcaa tactgatatt
     3361 gctttctttc ggacttcgtt ccaatgaatt cgcaaaatga aggaccatat aagcgatctc
     3421 ttcttcggat aatttttttt cttgaaaaat ccgctctact gctttacgga ttgcattcgc
     3481 aacttcctcg tattgaacca tgattttttc taaaatcgga ttggtcagtg tctcttcttg
     3541 taaaacggct cttgaaaaca caccagataa atgagtcaac agcattttgt acaaagtttc
     3601 atcttcataa aaatggacat tcgtctcttc acttacttct tgaatcagtt gttttactaa
     3661 ataagcaagc tgtgtatcga acgtctcttc aaagaaattc tgatcaaatg agttcgtgaa
     3721 gtcatttaat aatcctgcaa aaaagacgat ctcatttact ggataaagtt gttttgtctc
     3781 agaagcgatc cgtgaaaaca atcgtttagc tgtctgtagt aattctttgt ttgccagact
     3841 attatatgtc tcatttgtca gtaaatgatt tttagtcaca cgctccatcg tcaatgtcag
     3901 catcaaaacc aaatgttcaa gctttctatc acttagattg gtcttttttt ggatcaattc
     3961 ttccccgatt actttgcgtg caagagatag atgttgcgga tcaacgaatc gaaaaaaata
     4021 attcagtgat gcatcgaaag aaatactatg aaaaagttca tattcattga tttctaattc
     4081 taatacattc gctgtcagaa gccggcggta tttttccggt ccttcgatct cgtatccctg
     4141 gttccgaatg atttccaaag gcagttggcg aatactttct tccaactgtt tgatatctgc
     4201 ataaaaagtg gtattactga tgcgatatga ttccatgaaa tgagcaagac tcaacggctc
     4261 tgtcgtcagt aacaactcgg ctaaaatcgc gtgttgtcgt ttagcagtgg ataagtcatt
     4321 acttttccca tctgtatttt cttttaattg ctctaatact tcttctgatg cctcaatgac
     4381 aaatgagcct ctagctgctt ttttcaaaga agcatttacc gatttcaatg agacttcaag
     4441 attatctaat tcgcggtaaa tagtccgttt actgaccttc aataaattca tcattttttg
     4501 gatagataca ggtgacgggc tactgagaaa ttctgctaat aattgttttt ctctgggcga
     4561 taaatacatc ttatatttct cccttcacga gtttttcgta ttcattcgta gctacccaat
     4621 gttcaactag aactagccga gggatctttt cttctagctg ttcagttaca gctagttctc
     4681 tttgtgcgat ataaatcgtt tcttcttgga ttttgatttt ttctattgat tcttttgaga
     4741 taacaaactg ttttttcgct ttttttgcta attgacttaa aatcgtcatg cccatagttt
     4801 gtgagccgcg tctgttgttt ttatacaaaa atacaatatt agcaggcgac tttttttgtt
     4861 ctgtgatatc tgctatctct tttttttctt cccgctttgg aaattggatc gcttctttta
     4921 ttcgtgacag caagatatct gtttccaaaa agtgatccag tacatagatt tgactttgag
     4981 gtactttttc tcttgcaaga cccgctagtt catgttgcac gataaccagc tgatttactt
     5041 ggttattcaa ttcatagata gagctgtatc cgacagaaag atcgattttt tctgctgcta
     5101 atttcttttt cagcagggcg gctcccatag cacttgatcc catacctgag tcgcaagcaa
     5161 aaatgacgga atgaatgata gatggttcca agatttctcc cctttctttg accgttttgt
     5221 tctctttttc tttatttggt aatttcaata gtatcgctgc acaaatgaag gtcacaaatg
     5281 tactgattgc tatccctata cttaccccag ctagttgtgg ataaggcgca ttggcaaaaa
     5341 tcacgagtag tgacccaggc gatacaggtg cagctaatcc cgtatctaac cattgaaata
     5401 tcaacgtgcc gcttgcccca ccagccatta cagctaaaaa tagatacggt cgcagcaaga
     5461 caaatggaaa atagatttcg tgaatcccgc caatcgcatg gatcatcaat gctccactcg
     5521 catttacttt ttcctgtttt tctgcaaaac aaaggataga aagtaagatc cctaaaccag
     5581 gtccaggatt tgcctccatc aaaaataagg aagagtaccc gactgtttgt gagaattcga
     5641 ttcctaaagg tgtcaataca ccatgattga ttgcattatt gaaaaatagt acttttagag
     5701 gttctaagat cagactgaca agtggtaaca gattcattgt gactagccaa gacacaacgg
     5761 aggcgagcaa caagtgaaca ctatttaaaa ctggttcaat aaaaacgatt ccaattatac
     5821 aaataacgct accagtcagt cctactagta ggttgctgac aagcatttca tatccagcct
     5881 tgaatttcac gtggaatcta gaaacaaatt gttcatatac gatgccggca agcggcccga
     5941 taatcatcgc tcctacgatt tgtggagcat tggaacgagc gatcaaccct aaagaagcaa
     6001 ttgcaccggc gactgcccct ttttgtcccc ctaccgattt cccgcctgaa taactgatca
     6061 agatgggtaa aagcagctgg atcatccaat ggcatacctc ttctaatgtg gtgtgcaaat
     6121 cgccagacac accttttgtt gctaatgaca agattcccca agcgataaac agacttagat
     6181 ttggcatgat catttcattt aagtgatgtc cagcttttct cagttgtttc atagacacgc
     6241 gactgctgtt gtttgctttc catttgtatt ccatcttttt caccatactt ctatttattg
     6301 atggacggat tttatgtaga aaaagcagca aacgaaaaac cgtctgctgc tttcagctat
     6361 ctgggaacta tttgcctggt tcagtcgcaa tgattgctag ttcaccttgg accgcccact
     6421 cagaaacgcc atttggaata atacagcttg ttcctttttc taatggatat gtttttccgt
     6481 caacgacaag gtctcctgct ccttcgatta ccgtcatcaa tgtataaggg gcttgttttt
     6541 tgaaagatgt gattccttta atatcccact catatacatt gaaaaactca gtttctaaat
     6601 aagtgacgat cgatgaattc ccttttcgga cttctttgat ttgtaattct ggtgttttcg
     6661 cagggacagt tgttacatcg atcgattgtt ggatatgcag ctctcttgtt tgtccttgcg
     6721 catcttttct gtcgtagtca tacacgcggt aagtcgtgtc agaactttgc tgtgtttcta
     6781 aaatcatgat tcctttacca atagcatgga tcgttccgct tggtacataa aagaaatcac
     6841 cttttttaac tggtactttc ttcaaaaggt catcccagcg cccatctttg atcatctcag
     6901 ctagttcttc tcttgtcttt gcatgatgac catagatgat ttctgcacca ggttcagcat
     6961 cgatgatata ccagcattca gtttttccta attcaccttc atgtttcatc ccataggcat
     7021 catctggatg tacttgtaca gatagatcat cttctgcgtc taagatcttg atcaataatg
     7081 gaaacactgg ttctgaagga tggccaaaca attccttgtg ttctgaccat agttcatcta
     7141 atcttttccc tttgaattcg ccattttcta ctacactgac accatgagga tgcgcactga
     7201 tcgcccaatc ttctccgatt ttatcgttag ggatatcaaa accaaagact gaacgtaaac
     7261 gacttcctcc ccaaattttt tcttgaaaaa ctggttttaa aaacataggt tcctgcataa
     7321 ttcaaactcc tctcattttt catagctatt atagcattat ttcggtaata ccgctttctc
     7381 tttcaaaaag ttttcaacaa tttcatctcg acatatgaga tactccgcac gatgatcgac
     7441 aggatgtacg cgatttgaca aaaatatcaa cgcttcttgt gtttttatat caatcaagaa
     7501 aaacgtacct gtataaccag tatgaaaaag aatcgcatga ccgtcttcta catccttttt
     7561 taagtcccat cctaaagaac gatagccatt ttttttcggt gtttgatctt gcagtaaaga
     7621 atcaatcgtc tcttcttcta aaaaacgacc attacggatt tttcccttgt tcaaatacat
     7681 ttggacaaaa gtaatgctgt ctttcaaatc aataaagaga cccgcgttac ctgcatgttc
     7741 agccaaaact cttgctttcg gatcatgagt ttgtccacgc aaaacctcac ccgaaggcag
     7801 ttgttctgtg ggaacgattt ttttggaagg aaaaggaaga aataagctgt tcatcattcc
     7861 taatggttcc aatacttgtt cttgaaagat ttccactgca gtttcttggt aaatttcctc
     7921 tagcatcaac ccaagaagaa tcgtaccggc atctgtatat tttacttctt ttcctagttg
     7981 ctcacctggt tctagcttca aataagcggc gatcaattct tcctgagata gcttatctct
     8041 gtttggtatc catgttttga tatcagaagt atgtgtcagt aaatgacgca aagtgattgt
     8101 ctgattttca aattcaggaa gatatttatg aagcggttga tcgagttcta tctttccatc
     8161 ttctaataga cgtaaaacta atgaagtggt tgcaacgact ttcgtcagtg aggctgcatc
     8221 aaacaacaga tcttctgtca tcagctgctt ttcaggaacg acttgggcaa agccggctgt
     8281 aaatgtttct acttgatttt cttttatgaa ggcgactgct acacctggat aaaatccttc
     8341 ttccatcttt ttcaaaataa gctgtttcgt gtgtggatac atctttttct cctgcttttc
     8401 tatcagattc ttacagtcca agcttaaagc ttaatctatg aggaatcaac cttttttaca
     8461 caaaaaaact gcgcgaaaaa ccgcgcagtt tcccccggta tactattttc tctttctatc
     8521 tctagaataa gcgatagtat gaccgttcat aaaatgacga gacatgcgac aatcgttaag
     8581 aaagaaaaag ttaagcgtcg catcgaatgt cagttttatt cttttctttt ggctcactga
     8641 cgaaccacca ttcgattcct atagcatcat agattgtgag tactgtgtgc tttttgtcta
     8701 agaagaaatc aactgatagt tgctgcaagt gagcaatcag tccttccatg ctttcagtat
     8761 cgatcatgaa tttcagaaaa tcaagtccaa tcacttcatc tgtaggcaaa tcaattgcgc
     8821 ctccgctagc ttctgtcaaa ccaacatgga aatctccttc attcaataca tggatgccct
     8881 cttcttcatt atgtaccttc aaaccaagta catgattaag aaattggcgt tcttttgtta
     8941 agttagtaac atttaaatgg attttttcaa aataagaacc tgttgatagt ttactgaatt
     9001 tttcttcaga ttttcttaat aatgcttctt gatccattgt ttctggaatt tctctttggt
     9061 cgttcttatt ctcttcatgg taaatttcca attcattctc ttctgggtct acgagcaaaa
     9121 tccccatacg tccatgatct tctaaagctt ctttgattgg ataattcatt ttacggatac
     9181 gacaaaggat atctgccatt tcttctgtgc tagggataat caatgaaaaa cggttcagct
     9241 ttttgatcac ccccatgtga tcattcgctc taggactttc ttccagcagc aacatttcac
     9301 ttccagcttc ttctgttcct aaaatggcta attcgttttc ttctcttttc aaatcaaagc
     9361 caacaacgtt cttgtaaaaa tcaatcattt tatctcggtc tttcacccga atggctacac
     9421 tttttaaata ggtggcagcc cccaattgaa aatctttcat atttcttgct cccctctacg
     9481 tcaagcttta ttttaacgat ggattcttct tctttcaagc aataactttg attttgttaa
     9541 aaaaaatgtg agaaaattcg cattttgctt gttttttgta aattaatgag taaaaactct
     9601 tataaaaaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag ccaaacaaaa
     9661 atagctcctc ctatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
     9721 ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag atacaaaacg
     9781 gaaaaatagc ttctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga
     9841 acaccgttta ttcggaaaaa gggactgtga cacgactttt gtcacagccc ctaaaaaaaa
     9901 taataaattt gtactattct atgaagcttc ttcttcagaa aactgactgt tataaagatc
     9961 tgcatagaat ccgtcttttt ccatcaatgt atcgtggttt cctgtttcta cgatcgaacc
    10021 atgattcatc acaataatgt tgtctgcatc gcgtattgta gacaagcggt gagcaacaac
    10081 gaaacttgtc cggttttcca ataggcggtt catcgctttt tgaatcagta tttctgttcg
    10141 tgtatctaca ctagatgttg cttcatcaag gatcaggaca tctggatttg ctaagaatgc
    10201 ccgcgcaatt gtgattagct gacgctgccc ttgcgaaata ttgcttgcat cctcgtttaa
    10261 tacagtgtta tatccttctg gcaatttacg aacaaaatcg tctacgtgag cagctttagc
    10321 agcctcaatg atttgttcgt ctgttgcatc gtcgttccca tatttgatat tgtcatagat
    10381 acttcctgta aacagccaag tatcttgcag taccattgaa aactgtgcgc gcaattcatc
    10441 acgtgaaaga tcacgggtat ctacaccatc gtaacgaata ctacctccag aaacatcgta
    10501 gaaccgttca agaaggttga tcagtgtcgt cttccctgca ccagtcggac ctacaatagc
    10561 gaccatctca ccaggtttga catccaagtt aaaatcagtc attagcaagt tatctggtgt
    10621 gtagccgaat tgaacatttt caaaacgaac tttgtatggt gtatcttttt cttctggaag
    10681 gccagatttt tcattggaca tctcttcttc atccaacact tcaaataccc gttcggctga
    10741 agcaactgtt gattggatag tgttcatcaa acttgcaagt tgagtgattg gttgagagaa
    10801 ctgtgttgta tattgcaaga acgcttgaac atcccctaat gtcatgttcc catttgcaac
    10861 ttttactccg cctagtacag cgacgaacac atagcctaga tttttaacga aattcatcaa
    10921 cggcatgatg attgccgaaa taaattgagc tttccaacca gcggcataca gattctgatt
    10981 ttctttttca aatacttcct ggtctttttc agtatgattg aatgttttaa caatggtatg
    11041 accaccatat gtttcttcta cttggttatt caataaacct aaactttttt gctgatctgc
    11101 gaaaaacact tgtgatttcg gcgctacgat catcacgatg atcaagctta aagggatcat
    11161 caataacgcg actaacgtca attgccagct gattgaaagc atcatcacga tgaccccaat
    11221 caatgtgact gcactagtta caaattgagt caaattttgc tgcagcgtgc ttgcgatgtt
    11281 atccatatca ttgattgctc gagacatgat atccccattt gtatgtgtat cataatagga
    11341 aatcggtaca cgattcattt tggcttccaa atcatgacgt aattcataaa ctgttcgttg
    11401 ggaaacacgt gtcataataa attgctgtaa aaagttgaat acagcagaaa tcaaatacat
    11461 cacgattact gtcgcaatga tctgcgcgat tttatcaaag tctatcggaa attttgttac
    11521 ttgttggcct tgttgcatct gttgagcgcc agccattaca cctttgaata tttctgtggt
    11581 tgctttcccc aagacctttg gtgtttggat ttggaagatc acggctgcta ttgctagtac
    11641 aaatacagcg atgatcgcaa ccatacgttt agacatatag ccaaacaatc ggcgtaccgt
    11701 tccccagaaa tttttaggct tttcaccttt tacaccgaga tttctgccag gtccaccatg
    11761 tcctggaccg cctgctggtc catgagaggg acgtgtgttt gttttactca tgctaagtcc
    11821 tcctctctta gttgtgaatg aacgatttct tgataagttt cgtttgttgc taatagttct
    11881 tcatgtgttc ctttgccaac cagttttcct tcatctagca caaggatcat atctgcatcc
    11941 attactgtac ttactcgttg agcaactaaa acggtgatac tttctttcat attcgttttc
    12001 aatgcttggc gaagattggc atccgttttg aaatccaacg ctgagaagga atcatcaaac
    12061 acatagatat ctgctgtttt gactaatgcc cgtgcaattg ccagacgctg tctttgtcca
    12121 ccagagaagt ttcctcctcc ttgttccaca tggctatcta agccatcttc catctctgaa
    12181 acaaaatctt tagcttgtgc gatttctaat gctttccaaa tcatttcatc tgttgcatcc
    12241 ggcttaccat actgcatatt gtctcggatc gttccagaga acagaactgc tttttgagga
    12301 acaaagccca ttaattctcg caaattatat tgagtcatgt cactaatgtc cgttccattt
    12361 atttggatcg aaccagattc gatgtcatat aatcgaggga tcaaattgac taaggtcgtt
    12421 ttacctgaac cagtcccccc tatgattgcg acgatctcgc ccgacttggc ttggaaatca
    12481 atatcttcca atgcaagttt ttctgctcca tgatatcgat aattgacatg attgaaggcc
    12541 aatgttgcgt tttttccagc aaaagaaact gttttaggat tttcagggtc atggataccg
    12601 attttttcat ccaacacttc attaatacgg tcagctgaag cttgtgcacg aggcaccatg
    12661 ataaaaatag cggaaagcat cataaagctg atcaagatct gcatcgcata agtcataaat
    12721 gcaatcagat taccgacttc caaagtcata tctgctatgt agtgtccacc aaaccaagtg
    12781 attgctacgt ttgtaccgct catgatcaat gtcatcattg gcatcatcaa tgcgacgatc
    12841 gtattaactt tgattgccgt atttgtataa tctttattcg ctaaatcaaa acgattttct
    12901 tcaaagcgtg tcttatcgaa tgctcgaatc actctgaccc ctgtcaatcc ttctcgaaat
    12961 accaggttta aacggtcagt tttcttttgc atacttttga ataatggaac agcaaagtac
    13021 atgattccgc cgatcaaaat aatcattact gggatgacaa ctaagaagat ttttgtcaac
    13081 tgatgatctt tttgataagc aagtatgctc gcgccaatca acgtgattgg tgcattgatc
    13141 atcatacgta aaaacatttg tgtaaccatt tggatttgag tcacatcatt ggtcgttctt
    13201 gtgatcaaag aggccgttcc atatttatca aacgcatctt ttgtcagact ctctgatttt
    13261 ttatagacat ccgtacgcaa ttgcttaccc agtttctgtg attcacgagt agcaaaaaat
    13321 gtatttgcga tagcagctaa gatactgatc aaagaaaagc cgatcatcac aaaacctgta
    13381 tgccaaatat aatcgacatc tccttgcgct acccctttat caataatatt ggaggtaagt
    13441 gtcggcaaat ataaatctgc cagtacttgg atcaccatga aaactgcagc tgcaattgct
    13501 gaagtcaagg agatccgttt tacgattttt agcatctagt tcccttcttt ctatttatat
    13561 actccgtttt tcagccaaga aagtttcaaa ttgaattcac tgattgcttg ctctactgag
    13621 taatcttcag atgttgaaaa taagcgatac gaatgcgcaa ttgttgtaaa tacagtattc
    13681 atcactaatt ggatcagtaa ttccaattca tgatcatttt ttactttcag cttcgtccga
    13741 tcgatcaatt caagcaactc ttttgcatct tgtttgtgtt ctttatgtgg atcgtgaggc
    13801 gttttttggc gactcgtatg aggagcgact tttcttgagg cacggtaatc catgttcata
    13861 aacaagttac gataaaaaga agcattctcg ccttccaaaa tctcataaat cattttagaa
    13921 aaataggatt cgaatccttt gaatagatca ccatcggatt ctttcattag ttgtaacata
    13981 tctttagagc tgtcacgtct cattgtctca aagtaataaa aataaagatc ctctttatct
    14041 tcaaaatact gatagaaact gcctctaggg atatccgcta atttgacgat attagcaatc
    14101 gaagcttctt ttaagggtac tctagaaaac tcgattctcg cagcctcgat tagtcttttt
    14161 tgtttttctt taggcaaatg gaaaaatgtc tgtgtcggca tatggatcct cctttctttc
    14221 aagatgacac attgtcatat gacgctgtgt cattataaca cctcctacct gttttgcaag
    14281 acatttgcat agtttttttc attatcatcc gatacattaa aaaagtgata agaggaatcc
    14341 ttccatttga attaatgaga gttgcccatt aaaatagcca catactaaac aaaggatgag
    14401 ataaatggcc caatcaatta gtgtccaaga cttcaataaa ttgcctctcg atgctgacag
    14461 catcgtttta gatacaagaa ataccttaga ttacacaaaa aaccacttaa gccactcgct
    14521 ttctatcccc gctgctatct tgccaagaca attaaaaaat ctagatccaa agaaacttta
    14581 ttatattcta agctatacag gcaggcgatc agaagcatta gccgatcaac ttactgctaa
    14641 tggttttcgt gctgtttata tcattggtgg gatggaagct cttcagtcga gtgttgcttg
    14701 aatattgctt gttagattca aaagaggctg tgacaaaaaa tcgtgtcaca gcctctttta
    14761 taccctgtct ttttttagtt atttattctt caaccttatc aatatgactt gatagccttt
    14821 tctttttctc atagttataa caatcatagc aataaaagga gaaggtaagc atatggcatc
    14881 tgttttagta gtcaaaggtc atcccctaac tgcagaagaa tcgcgcacag ttaaggcatt
    14941 gacgtctttt ttaacaagtt ataaagaaaa tcatccagat gatgaagtga ctgttttgga
    15001 actttatcgc gatgatattc cggaaatcga tgaagaatta ttatcaggtt gggaagcatt
    15061 acgtgctggt gcagaattca cttccttatc agaaagtcaa caacaaaaaa ttgctcgttt
    15121 caatgaattg actgatcagt tccttgctgc tgataaaatc gttatcgcaa acgccttatg
    15181 gaatctaaat atcccaacaa aattaaaagc ttggttcgat accgtcaatg tagctggaaa
    15241 aacgttccgc tatacagaaa acgggccaga aggattagtg acaggtaaaa aagcactgca
    15301 catccaatct aatggcggtg tatacaacgg acaagacttt gcctctcaat atgtaaaagg
    15361 tattttaaac tttgtaggca tcgatcaagt agatcagcta ttcatcgaag gaatcgatta
    15421 tgaccctgac cgtgcagatg agttgatgca aaatgcatta gataaagctg ctgctttagg
    15481 taaatcattt taaaaattga gataaatgca tacaaaagag acagaggaaa aaatcttcaa
    15541 agcagcattt gtaccttgga tattttccca tctgtctctt tttgttattc tttctgcttt
    15601 ttcaacggat tttcttcata aagagaagag attgttgtgt accattcaaa aacatgagta
    15661 atcactactt tagctgctgc ataaacagga atccccaaga tcacaccgat gattccgaac
    15721 aatttcccag aagtcagcag aactaataaa atcgttactg gatggattgc taactgattc
    15781 cctagtacta atggagagat caagcgtcct tctattgtct gctctactgc aaaaacgatc
    15841 agtactttga tgatcatgaa tggacctgct acaatggcta aaaagatagc cggaatcatc
    15901 gctaaaaatg atcctaagta agggatcagg ttcaaaaaac ccgcaataat ccccaacgtg
    15961 acagcatact ctaaaccaat gaccgcaaaa ccaatcataa acatgattgc cacagcaaaa
    16021 gccacagtta gttgtcctct tatataagaa gaaacttgac tattcatttc actcagcaca
    16081 ttcaacgtcg gtttttgcat gcgtgtcggt aaaaatttga caaaataagg tgctaattgt
    16141 ttaccgtctt tcaatagata aaataagata aacggcatcg tcagcagagc cactaaaata
    16201 gtcgctaccg cgccgacaaa acttccaaga ctctgaactg tactcttaga tatatcttgg
    16261 atcatatcgc ctgccgaatt catgaactta tcacctgctg tttccaactg ttcgcgaaat
    16321 tggttgaaca gcgggtcccg caaaatctcg gttaatttat tatcaatcgt atctacatat
    16381 tgagggaagt tactgatgaa gctcactgtt tgttcctgaa tcttagggat gatcactacg
    16441 ctcccccatg cgattagtgc aacaaccaaa acaaacagcc caatgatact gtaaagcctt
    16501 ggtacttttc gcttttcaaa gtaatcaact accggattca tcaagtaata caagattcct
    16561 gctaaaatga taggcagacc tacgattgct aaaaattgcc agattggtgc aaacaaatat
    16621 gaaacttttg taaataaaaa gacgatcagc aatattaaca atacaacaag taaggctgtg
    16681 actacttgat tatttaaaaa ccagcgccaa aaccacgata ccggtctgtt cttcttattt
    16741 tttccttcca taattcctcc tgttagttat aaaaaatttg gcttcccaaa ccaatagcag
    16801 gcagtctatg cggatgaaga tagattccat ttgcaatttg tccttcttct ggacaagcat
    16861 caaaaatcaa tgatacgtga gctactgtct ctaaattccc attggccatc ggaccgacat
    16921 aatcgatcgt atatacctga tcatcaaaag caattgttcc gcctgctttc aaagaaaaag
    16981 gttcgtcttt tgatacttct tgaatcaccg aatattttcg taaggcagct gttgctgttt
    17041 tatcgaacaa aatcagcatg ggttcttcct tgtttattgc ttctgtccca atttctgtaa
    17101 tctttgcctg aatcattgca ctctctcctt taattgcttt caaaaacagt ataacacatt
    17161 ctcattcttt agccgtgtgg aataattatg tgatagaata aaaacgaaaa ctatttcatt
    17221 acaattagga ggaatcagct catatgatcg aaaagataat tttaggaacc tatacgcgtc
    17281 gcgaaagtaa aggaatctat acgatcgacc ttgatacaga aaaagaagaa ttagccaacc
    17341 tttcattagc tgttgaagaa aatagcccta cctatgtagc aaaaagtaaa gctggcaatc
    17401 tttacacagt tactagtgtg gatggattag gtggtgccgg tgcgtacgat aaggattttc
    17461 attttttgaa tgcggtaaca gagtctggcg ctcctttgtg ttatgtagcg gttgatgaag
    17521 cacgtcaatt actatacggg gcgaactatc ataaaggtga agtaaatgtc taccatatat
    17581 taggagatgg cgaactgaaa gcagcagatt caatattcca tcaagaagcg acaggtcctc
    17641 ataaaaacca agatcatgcg catgtccact atacggatct gactcctgac caacgattag
    17701 tcgtatgtga tcttggtaca gatcgtgtgt atacttatga tgtgtccgaa aacggcaaat
    17761 tgaatcttgt aactaccttc actgcagagc caggaacagg ccctcgccat cttgtctttc
    17821 atccaaatgg acaattcgct tatttatttg gtgaattaga cagcacagtc cgcgtgcttt
    17881 cttataataa agaaacaggt gcttttgaag aattacagaa aatatcgacc ttgccagaag
    17941 aattcgacgg tgaaaatgga ggggcagcta tccgcatttc aaatgatggc aagttccttt
    18001 atgcttctaa ccgaggacat aactcgattg ctgtattcgc agtttttgaa gatggttccc
    18061 acattcaaaa caagcaaatc atctcaacag aaggggattt tccccgcgat ttcgcactag
    18121 atccaagcaa taattttgtc gtgtgtgcca atcagaacac cgataatctg acattatatc
    18181 gccgtcatcc tgaaacagga ttactggaat tactgaaaaa agatgtctat gtaccagaat
    18241 gtgtctgtgt ttattttgat cgttagtaag aaaaggagaa tgaaatgaat cagcaagacg
    18301 tattagacag actacgggaa gaactaaata ttcctttctt tgatgggaaa atcgaagata
    18361 aagagtatag cgaagaggaa tatcagaaac tcaaagccga tttacagaac tactttgaac
    18421 aatatgtaca gaatgtagaa aactaatcag ctttccatac tatctgaaaa gaacatcagg
    18481 acaagacttt tccgtctatc ctgatgttct tttctacaaa taaaaaaccg ccgagatggt
    18541 tagactcgac ggaaaaggag tatttactcg attttgagta agtacaaggt tgttgttggt
    18601 tataggtatg tcattatcat aaaggatttt gatcactcta tcataaaggt aatattacat
    18661 ttatattaga taactctagt ttttattgtt atcaaagatt tcttttattt tttcaacaac
    18721 aaattagtca ttttttggat ggcagctgtc aattggtctg tcgcttggtc ttgttttgct
    18781 tcagatgtat tgaatagatc ttcatctacc cctgattcaa gatcgatact ttggatcatt
    18841 tcttcacagc ctgctacata tgaaagataa gctttttcaa aatttttatg aatgcccatt
    18901 gcttttgctg gtggacgcaa agtactgact ttcttaagca ttgcccgata tttttttgtt
    18961 ccttcttcaa ataacaggat gatctcttca agagtcgatt tgtccagttc acttgttttt
    19021 ccttcatcaa ttgcgtttct gatcacttca tatttcggat gcattccttc tccgattgct
    19081 tctgtttcct tgacgatttg atcgatggac tgcacataat agccaatatt cggtaacatg
    19141 ttcgttttcc tactttctcc attgttgtta ttatttattc tacttgtttt tatcctcaaa
    19201 tgacaagtac ttgaaaaaat ttaatggttt ctatacagct cgccattctt attcgtgctc
    19261 aaagaaacca tctgctaaac tatattgttt ccctgcgaca atgtcatatt ggatcaattg
    19321 atattcgtcg taaatctctt gttgcgagcg attgaattgc gcttctttat accactgtcc
    19381 gttttgatag tacagttctc gttcaaacgc cggaagttcc tgttctaatg ataaaagcag
    19441 ctgataaaag ccatttgttg tctgattcgt ctgttccatt agattcgctg caaaataaaa
    19501 cggactggtg attgcatcgt gagtcgtttg cttttctaat tctcctttac tgtcgaacat
    19561 aagaaattgt gtttcatgca atgtttgttt gtcatttttt gcttggatcg tatctgaata
    19621 gatccctgga agatgatccc cccaaaaaac aactaaagtc cttctagaaa gattcttcaa
    19681 ttcttccgtg aactgcttca gtgccgtgct gctataggaa atatcttgca gataattctc
    19741 taagtcaagt gtcccacttc catctgaagt ttttgcagga tagtctagat tatcatactt
    19801 tccatcgtag ggcatatgtg tctgcatcgt gactaaatga atgaactgag gtgttttttc
    19861 ttccttgaga agtgtcatta cttctttgta agctgactca tcagaaatat atggattatt
    19921 ctctatcgta tccgtatagg tcatcgtacg ctcgctgatg aactggtcaa atcctaatgt
    19981 ctgataaaca tcctctcttt tatacatcga agtattgtat ggatggatcg ctgtcgtctg
    20041 gtatgattgt gcgtttaacg ctgaaacgat agaaggaagc tgatccattt ttggtacaag
    20101 catcgtgtaa ggggtggtga gttgcgcatt aaataacgcc atagaaaaac cagtcaatgc
    20161 ttcaaattcg atatttgctg ttccaccgcc atagttttga gaaagcatat tgccactata
    20221 tgtttgatca gctacttcat aataatctgc caatggctca ccagatactt ctacgccgtt
    20281 taatcgacta ggatctgaga aactttcact catcacgaac acgatgttcg gcgactcttc
    20341 ctctacagtt ttattttttt catcggctag cttctggtat ttttcagtga tttctttgat
    20401 cttttctttc gaatatcctt caggttcatc cattggttct accttcagat tgtacaaaaa
    20461 cccaccgata aaaccagtgt tatagtagtt cattttctgg ctgtaaggta tccatagtgc
    20521 cgttttatta tatgcttttc gcaacaaatt atcgggattg ttgaaattac tgatataccc
    20581 tagcagtcca attgccacaa gcagtgtcgt taatcggatc aattggaatc gccttccttt
    20641 tttcaggctt cgaaacatgt accagcaaaa caatcctatc acggttcctg cggcagctaa
    20701 gatcactgta aacagcatag tgccggtcat atctttcagc aatccgattt cagtgatcat
    20761 cttcaaatcg tctggataaa tcggttcttg ccgataactc attttcatat aatttgcaaa
    20821 acctaataca ccgatggcta cgacatacgt aagggaacct aaagggacag atcctgctag
    20881 tgcaatcaga aaaataagaa taatcagcaa gacgagacag gctaagaaga atttttccgt
    20941 atgccaagaa aaagcaaatt tcaacgcaag gtctacggat agatcatttt gacaccactg
    21001 caaaaataaa ttactaaaaa caacaagaaa aaagatactg atgattgctg ctgttccttt
    21061 taaaatcttt ttctgtttgg tcgaaagtct gcttatatat cttttcaaat gcttgctcct
    21121 tcatttctat caataaaaga atgttgcttc tcttatttta gttgtacgaa attcgaaagt
    21181 aaagaacgga aagaagaatt aatgaaatcc aaatattctc tttctttatg gcctttctgg
    21241 tttctttaat aagcggacct ttcgtactcc agaaattttt tctagctgtt gtttcacttg
    21301 attgagctgt tcaaaatcag tactgtctat gtcaatcaat atgtagacat gctcattctt
    21361 atggttacgc gatatatctg cgatcgctag ctcgttctca ttcagcagag aaaatatctc
    21421 tgctaatata ttggttttct tttgataaaa tatagtgaat cgaaacggcg cttggaaaaa
    21481 cattcgtgcg gatggataat tgaccgcttc tctaaccgta ccgaacaaaa gataatcttt
    21541 taaggcttca actgccaaac gtccgctgtc tgccaaagct tcctgagtcg atccccctag
    21601 atgaggcaac ataagtatac ggtcattttc taagaactct tcttctggaa aatcagtcaa
    21661 gtatttagcc agatgttgtt tttccaatgc ttgcataaca gcatatttat caacaatttc
    21721 acttcttcct acattgataa gtacagcatc ttttttcatt ttttctatat tcgcctgatc
    21781 gatcaatcct tttgtatctt ctgtcaacgg cagcatgact acgatgtaat ccgaggtaga
    21841 aagaactgtt tctaaatctg cttgccgaac ataatccaac tgggcatcgc ggatagaata
    21901 acccaagaca tccatcccta agctatagca acttagcgct acttttgtgc ctatcgctcc
    21961 aagaccaaga agaccgatgg ttttgccttg caattctcgt cccacataag cacttctttt
    22021 gttttctgcc tgttctaaaa tattaggacc tgttagtgtt tgtaccatac gacttgcttc
    22081 gattatcgga cgggaagata agagcaaaca gcacaaaaca agctctttta ctgcattagc
    22141 atttacacct ggtgtattca tgacgatcgt cccgttttca gaagcttttt caacgttgat
    22201 cgtgttcacc ccaacaccag ctcttgaaat tgctagcaaa tctggtgtaa gccattcatc
    22261 tttcactttc gtactacgaa taactattcc ttcggctggc aaatcatcta ttattttgaa
    22321 atattcccct tgtaactttt ctatctcttg ttctggaata gcatcaatca aatgaacgtg
    22381 atacataaaa ccccctcctt actaacaagt atctcgaaat ttctgcaatt agacaactgt
    22441 ttaccatttg acttcctaga agcaaaaaga gcagttagag cagagtaggc tgagtgataa
    22501 aaataaggct atgacaagtt actgccataa gccttatttt cgagagcaaa atattgaggg
    22561 tgagatatct tgataaatgg tgttccgtta gctgacattg ataataagac taaatttcaa
    22621 aaaagttgaa aagcaaattt cgtcaatctc ttcttatttc ttaaaactaa gtggcttttt
    22681 cacaacctct ttttatctaa taaccactgt ttccaactca actaattttg cccacgtttc
    22741 aatttgatct gttgttagat ttagcgagac aacggtatga tgaccgccac ctgcctgaat
    22801 ccatgaatgg acaccttcat ggaaattcgg tttagttttc cataacacac gtgcaacagg
    22861 taaattaggt gcttcagtcg ttggttcaaa ggcttctact tcattgatca gcagtttata
    22921 atgcgtaccg aaatctgcca tcgatacgac taccccttct cctgctttcc catcaaatac
    22981 aagacgcgcc ggatcttctc tatctcccat tgataaagga gaaacaacga ttcttggttt
    23041 cgtcgcagca agcgtcggat ctacttccat catatgggat tccaagattg cttcttggcc
    23101 actagctagc tcgtaagtat aatcttccat gaatcctgta tctttccctc gagccatgat
    23161 tttcagcaac cgatcaatcg ctgcagtttt ccagtcgcct tccccagcaa aaccgtatcc
    23221 ttctgccatc aaacgttgta cagctagtcc aggtagttgt ttcattccat acaggtcttc
    23281 aaaattagtc gtaaaagcgt tatagcctcc ttcttctaag aaacggcgaa taccgatttc
    23341 ctgctgagct tgtactttta catgttcctc ccatgtcttt tcttcataat caccatagtc
    23401 gaaggtatac aattccttgt actcttcaaa taattgatcg acttcttcgt ctgatacgcg
    23461 atcgattact tgtacaagat ctccgatacc aaaataatca acagtccaac caaattgaat
    23521 ctgtgcttcc actttgtctc cttctgtgac acccacgttt ctcatgttgt caccaaaacg
    23581 agccactttt attccaaaac tttcattata ggctactgca acatccatcc aatcagcaat
    23641 ctctttttgg atctcaggtc gtttccagta gccaacgacg attttatttt gtttatttaa
    23701 tcgagcattg atgaacccat attcgcggtc accatgagcg gattgattta aattcatgaa
    23761 atccatatcg atcgttttcc aaggaatgct ttcgttatat tgcgtagcta aatgaagcaa
    23821 tggtttttgt agaagctttg tcccgcggat ccacattttt gctggtgaga aagtatgcat
    23881 ccaagtgatc acacctgcta cttcttctcg ataattgacc tctttcatga tttttgtgat
    23941 cgtatcagca gtaactgcta attcttgaag gcggattgga taaggcaatt ggccattttc
    24001 attcagttca tcgaccattt cttgtgcatg tttctttact tctcgcagtg cttcttcccc
    24061 atataaatgc tgtgatccta caacaaacca aaattctttt tctccaatat tcaacataat
    24121 tagccactcc ttttatttgt tttgtccgta atacgcattt tttccgtgtt ttcgtaaata
    24181 atgtttatct aaaatccgtt gaggcagctc ttctgaaaat gaattgagct gtctggtaaa
    24241 taaattcatc ttgcatactt catctagtac gactgcgttc attaccgcac tttgcgcatc
    24301 tttcccccaa gtaaatggac cgtgtccgtg taaaagtacc cctggaattg ctaagggatc
    24361 aattttgcgt tctttgaatg tttctatgat aacttttcct gtttcatatt cgtacccgct
    24421 gtcgatttcc tgttgtgtca aaaatcgagc acaaggtacc gatccataaa aagtatctgc
    24481 atgcgtcgtc cccatcgcag gaacatccaa tcctgcttgt gcccagatcg tcgcccatgt
    24541 cgagtgagta tggacgattc ctccgatttg aggaaatgaa cgatataaaa cagcatgagt
    24601 aggcgtatcc gaagagggat tgagtttacc ttcaatgata tgattgtcca agtccacaac
    24661 gaccatgtcg tctgctgtca tctcttcata actgactccg ctaggtttga taacgaaata
    24721 tcgtgtttca ggatcaaaag cgctcacatt tccccatgta tatttgacga gtccgtgttt
    24781 aggcaagtca agatttgctt ggtatacttc ttctttcagt tgttccagca ttttctatca
    24841 ctcctttttc ttgcaattga tgaatcgctg ttttttcaat gtcgatcccc tcacggtaac
    24901 gtctcataaa tatctgatag cctgctattt cttctgctga aggtgtgatg gtgattcctt
    24961 ggtcttgtcc aaacacgtct tctgccaaga agttttctaa agacttccca tgtcgatcca
    25021 tcatataggc tgctaataac gcgattcccc acgctccacc ttctccggct gtgtccatca
    25081 ctgtaactgg tgcctccata gcagatgcta gaatccgctg tccaacttct ggtgttttaa
    25141 aaatcccccc atgccccact aaacgatcaa tttggacatt ctcagatttc aatatatcca
    25201 ttccgattct cattgcacca aaagcactgg ctaaatggat ccgcatgaaa ttcgctaaat
    25261 caaaacggct acctggtgta cgtacaaata atggccttcc ttcatttact cctgtaatgt
    25321 tttcaccgga aaaataacca taagaaagta atcccccgca atccgtatcg ccttctagcg
    25381 ctttgttgaa taataccgtg aataatttat ctctgcttat ctccataccc aaagaattgg
    25441 taaattcttc aaaaatactg acccaagcat tgatatccga agagcagtta ttcgtatgaa
    25501 ccatgcctac tagttctcct gaaggtgtag tgaccatgtc gatttccgga tggacatttt
    25561 ctagttcttt ttctaatacg atcatagcga aggcggaagt accagccgaa atgttccctg
    25621 tccgtttggc tacactgttc gtagcgacca taccggttcc tgcatcccct tctggcggac
    25681 aaaccgggat tcctggtctc aaatttcccg tagggtcgat cagataagcc cctgattcgc
    25741 ttaacttgcc agcttcttct cctgctacta gaactttagg caaaatgtga tccaagtcaa
    25801 tcgctacgtg cgctttttcg attaactgac caaaaatatt tttcatttct tgatggtagg
    25861 ttttcgttga aggatcgata ggaaacatgc ctgatgcatc tcctacgcct agtactttct
    25921 caccagtaag ctgccaatgg acataacctg caagcgttgt caaaaaatta atttctttca
    25981 catgttcttc tttattcaaa atagattgat acaaatgagc gatactccat ctctgcggaa
    26041 tattgtattg aaatttttcc gttaatattc gttctgcttc ttcagtgatt gtatttcgcc
    26101 aagtacgaaa tggtactagt aactgttcct tgctatcaaa tgctagataa ccgtgcatca
    26161 ttgcactgaa cccaatcgac ccgattgtcg taagattctc gccatagatt tcgaatactt
    26221 ctgctgccat ttttcggtaa ctgacgcgca gtcctttcca aatatcttct aatgaatagg
    26281 tccagatacc attttctagt ttgttttccc actcataact accagcggca ataggagtaa
    26341 aagatgaatc aatcaataca gctttgatcc tagttgatcc aaactcaatc cctaaagatg
    26401 tacgaccatc tatgacatct aacatacttt gcgcatttac ttccttcata atgatttaac
    26461 tccctccttc tcgtcaaacc tgatcaaacc gtctttttag tagcgcttac aatgattatt
    26521 gtatactttt gtacgtacat gtcaagcggt tatttacaag aatacaataa aaaacacttg
    26581 ttttgtacgt acataagtta taaaatgaaa ttgcttacaa aatctgatca accgccttct
    26641 cgaaattcta gaaggagaga atacttatga aaaaagaaaa aaaagtttcc tcatcgttta
    26701 tttatttttt tggcgcattt ggcggcattc tatttggata tgatattggt gttatgaccg
    26761 gcgccttacc ttttttacag catgattggg gtttggcagg taaagcttct ttgataggat
    26821 ggatcacgtc ttcggtcatg ttaggtgcga tccttggcgg ctctttatct ggtcttcttt
    26881 ctgataagct tggacgacga aaaatgattc ttctttctgc acttattttt atggctggtt
    26941 ctgtcctttc tgcatctgcc cctcacaatg gttcttattt tctgattgca gcaaggattc
    27001 ttctaggttt agctgttggc gctgcatctg ccctagttcc tgcttatatg tctgaaatgg
    27061 ctcctgctag actaagggga agattgtcag ggatcaatca agtgatgatt gcttctggca
    27121 tgctcctttc ctatgttgca gattatttgc taaaagtgtt gcctgaaacg atggcttggc
    27181 gcgtgatgtt agggctcgct gctgtccccg cgctgatttt atttttcggt gttttagctc
    27241 taccagagtc accgcgtttt cttatgcaat ctggccgctt ggaagaggca aaaagagtat
    27301 tgaactatat tcggacacca aaagaagctg aacaggaatt cgaacaaatc cagctgaatg
    27361 tcaaacaaga aaagacaacc ggaacttctt ggcatacttt gtttttagaa aaatatcgtt
    27421 ccttagtcat tgccggtatt ggtgtagcag tattccagca atttcaagga gctaatgcta
    27481 ttttttacta tattccgctg atcgtagaaa aagcgactgg gcatgcagcg agcgatgcat
    27541 tgatgtggcc gatcatccaa ggaatcattt tagttgctgg ttctttgctc tttttggtta
    27601 ttgcagacaa gttcaatcgg cgaactttat tgaaaatagg cggtagcgtg atgggacttt
    27661 cattcatcct accagctgtt ttaggaactg tactggatgc tcatacaaat tcgttgttga
    27721 ttctcttgtt tttatgtatt tatgtcgcat tttattcttg cacatgggct ccactgacat
    27781 gggtaatcat aggcgaaata ttcccactag ctgtccgcgg gcgagcatct ggcctagcct
    27841 cttcttttaa ttggattggt tctttcctag ttggcttgct ttttcctgtg atgactgctt
    27901 ctatgtctca agaaatcgtt ttcggcattt ttgggatcat ctgcttttta ggggttttat
    27961 ttattcaaga gatcgtacca gaaactcgtg ggaaatcttt agaggaaatc gaacaaagtg
    28021 catctaaaaa aacttatcct aaacgtattt ctatccatca ttcttaaaga ggttgtgaca
    28081 gaagtggtca gcttcaagaa ataagaagga accgcttagt ttccagtcac aaggaacaat
    28141 ttcaaaaaac agcttctcat gtttgatgaa tattgtgact tgtgaccagt tggtaggaga
    28201 acaccgttta gtggagaggt ggtgaaagaa acgttcagcc ttgagcgatg acaaaagctc
    28261 tcacaaacat tgcgacgagt aaccgtagga gcaataagag gcggtaaaaa cccgtctctt
    28321 atttttcgtt ttctttcact gtttcccgtt caactagtac aggttcatag agaatatttg
    28381 gctgtgcctg tcctttttca atcgttgtga cgatccaccg tgcggcatct tgtcccaact
    28441 tttcttttgg atgcgtcaat gttgtcaatt ttttattccc taaatggctt aaagaagaat
    28501 cgtctgttcc tacgatcgat aggtcatctg gaatatgata gccattttct gtcaacttat
    28561 ctagcattaa actagctatt tgatcgttat agcaaatgat gccatctaaa gcttgttctt
    28621 ctttcaaccg ttgtactgtt ttttcagcga ttgtttctcg tgtttccgtg gtgaaagtaa
    28681 tgatatcttc cgtcaaaaaa cgcacattat actgttcaca cgcgctgata aaacctttca
    28741 tcctgtattt tccttgaaga tcgtcgattt ttgtgatgaa caacagacgc tgatgtcctt
    28801 tttttatcaa atattctgct gccataaaac cgactttcac atcatctaaa caaatataag
    28861 aggcatttaa ctcttcataa gcagcattga tcatcaccat cggaatgcct tgttctctca
    28921 aagcaacata aagtgctaag tttgggttat actgattact tttcgtaggc tcaacgatca
    28981 aaccatctac cccataagca atcattttct tcaaacactc tttctccaat tcgtgatcgt
    29041 tattcgtact ggctaaaagc aatgaatacc cagattctcg taattcattt tcgattcctc
    29101 ggataatcga cggaaaaata taatcagaga gatatgtggt gatgacaccg atcgtttttg
    29161 ttctgttcaa tggtgttttt tctttcttat agctgtcatc tacatatgtg cccgaccctt
    29221 tttctttacg cagatagccc tcatttacca acacagcgat tgcttgccga atcgtatgtc
    29281 tgctgacgtc atatatttct tgcagcttga attctgaggg aatggttgta tttacttcgt
    29341 attcctcacg aaggatcttt tgttttatct cttctgcaat atcttgatat tttgccattt
    29401 gccttgcccc tttttatttg tacgtacata tatcataacg agtctgcaag gtttattcaa
    29461 gcaaaaatca aggtttcttg tgctcataag gcatacggaa caggttcacc aaattctgga
    29521 aatccttgct cattccacgt aaagcattgc gcgttcgcgt gccgattagg attgtctaac
    29581 ggatcccctt tttggttctt ctctggtcga gcatgataga tcaaaatatc aaagcacccg
    29641 tcttctgaaa ccgtaaacga gttgtgacca ggaccaaatt gcttattttt ttcagaagaa
    29701 acgaatactg gctccgataa tttatgccat gattctttta ataaaagatt cttgtgctca
    29761 tcagcccaca gcaggcccat ggcatagttt tcatctgttg cactaccaga atacgtaatg
    29821 aatattttcc cattgcgagt aaggactgct ggaccttcat ttactgaaaa accccgtttt
    29881 tcccattcat actcaggtat agaaaggagg atctgcttcc cctttaacgt ccaaggatta
    29941 tccatttcgg atagataaag attagaattc ccagtaattt caggatcttg ttgggcccac
    30001 acataatata gctttccttg agaagagaaa gttgttccgt ctaaactgaa agtttccctt
    30061 ttcgttttta tttgtccctt ttctatccaa tctgtattca ttggatctat atgctgattt
    30121 tcaagtacga acatgcggtg atgatgagat tcatcacgta tagttttatg attgcttgca
    30181 gcaaagtaaa tataccattt tccattgata taatggactt caggtgccca gatcaattca
    30241 ctcatcggtc cttcttcatg agcctgccat acaatcaggc ttgcagcatg ttctaattca
    30301 ttcagtgact ttgcccttct taattcaatc acttggtagc ctggaaccga tcctgtaaaa
    30361 taataataac catctgtatg tttatatacc caaggatctg cccgttgccg aacgatagga
    30421 ttattataaa gtgtcttcat cataacaaat ttcctcacat tttctttatt ctaattacat
    30481 tccaagagta tttttctatc cctacattta attcgtcatg tttttctatt gaaaaatagt
    30541 ttatttcagg ttttattttt tctggttctt cacgtgtatt tttgtccaac agatcgctac
    30601 tggttaatgt gtagtgctct ccttgatcta gcaaattgaa atcatgttca atcgttatct
    30661 tttgttgttc atttgtgcga ttgatcaaga aaatagtcag ctgattgccg ttatctacga
    30721 tcacactgtc cacataagga acatttttta tcttttcaca atcataaaca gggcaaatga
    30781 tttgttgttt caatacttga cctttagcat atttcgataa gtgttgcatc acataataag
    30841 ttggattcct ccatgcttgt cctttagcat ctgtcagtat taaaggtatt gtgtttacta
    30901 acaatgattg acaactaatc tttacgcgat ctgcgtgtcg taaaatagcc agccctaatg
    30961 aaccaaataa taatgtatct tccatagtaa agtaacacca atcgatggga ctaccgactt
    31021 caaaatccct atgtcttttt tccggttggg catgtacatt ccattcatca aacgctagat
    31081 acattgtttt ctcacttctc aaatatcctt ttacataatc acaagtagca acgatttctt
    31141 caatttgacg atcgaatcga taagcttttg caaggtatgt cgctgtatca tcacgctcat
    31201 ttttaggagg tttagtcaag tcttcttcat catacttatc aatatagtta tgcaaagcta
    31261 aataatctac attctcataa gcttcttcta atgcgatacg atcccattca gggtacgatg
    31321 gtaaacgtgg cgtagagctt ccaactaaga ttgtttcaat tgaatcatcc actaacttca
    31381 tgacttttga ggcttcattt gctaacctac catattcata agcactctta gcccccactt
    31441 gccatggacc gtctagctca ttccctaaac accataattt aacgccaaaa ggcttttttt
    31501 gcccattttt tattcttaaa tcactccaga aggttccttt tggaaaatta caatattcta
    31561 ctaaattttt tgcttcatca attccttttg ttcctaaatt caccgtgaat atcggtttcg
    31621 cttgtacatg actgatccat ttaaaaaatt cgtggagtcc aaaagaattt ggttctatac
    31681 ctcgccatgc taaatcaatt ttttttggtc gttgttcttt cctacctaca gtatccaccc
    31741 aatcataacc agatgtaaag tttcctccag gatagcgaat gacacctaat tctaattcct
    31801 ttacataaga gaggacatct tgtctaaacc cgtcaccatc agctgtttca tggcccggtt
    31861 cataaatccc tgtatagacg acacttccca tatgctcaac aaatgagcca aacagccgag
    31921 aactgatctc tcctaccgta aaatcctctc tgatttttac tttcacttct tcgtccaaca
    31981 gctttcccta cctttcattt cctatagtca agataataga tacaagtttc catacgccac
    32041 cagcaaagat aaaaataaaa ggagggaaaa gaaatatgag cagtacaaat cctaataaca
    32101 atacgcaaca aagaatattt ctcaaaaaat caaaaaatac tttccatgca ctttgttcaa
    32161 aaatttgtaa aaatgtgata tctttgtttt cagaaatcac atatatccag gataaaagaa
    32221 acatggaata aataagtaaa actacatatc cactaattga aagtacgatt gtctgttctt
    32281 ctaataattg attaacaaga aaataaataa tgatcgttaa cagactaagg aacaagttct
    32341 taccaaaatg ttgctttatt ccttgaaaga aaaaataaat gatatttcct gatccctcac
    32401 ctttccattg cgccattaca taaaaaacag cacatgtagc aggcacgata gtaataacag
    32461 gcaaacacac gattacccag agaataccta ataagatgta atcagaaacc acaatcaact
    32521 gtccataaaa tttagtctca actattttca tcttcctcac tctttcattc cagacatagc
    32581 tgttccttga acaaaatatt tctgtgcaaa aatataaacc actagtaatg gcagcattga
    32641 aataaccgtt ccagccatta caggtccata aaaagaaatg ttctgccctg taaataaagc
    32701 taaccctgct ggtaaagttc tcatttttga actacttgtc aatatcaatg gataaagcaa
    32761 gtcattccaa ctattcatca gcgtgaaaat ccctaatgat aaaagagtag gtttgctcaa
    32821 tggcaacatg attttcaaaa aaatattaaa ctcacttaat ccatcaatgc gtgctgcctc
    32881 ttccaaagaa tctggtaaag taataaaaaa ggaacgcatc atgaagatac caaatgcagt
    32941 tgtcatacga ggaatgatca aacctgcata agtatcaagc atgccaagca aattgacttc
    33001 aataaataga ggaatcatga agacttggaa tgggatcatc attgtcagca aaacgaaata
    33061 gaacaaaact gattttcctt tgaaacgcat acgagcaaat gcataccccg caagagaatc
    33121 aaagaataca gaagtgatca ctgtccctcc agcaaaaatc actgtatttt tgatgtaatc
    33181 taataaaggg atcgttgtcc aaaccttaat atagttatct agggtatagc tatctgcaaa
    33241 tattctaggc ggaaatgaga taatgtcttt ttcgtgttta aaagaagaaa taatcagcca
    33301 caaaaaagga aacgcaataa ccaacaatat aactagcata ctaatcgttc gaatgatttt
    33361 tttaccacta actgcttttc gactattcac agtaactgta atcgagcttt ctggtgccat
    33421 acaggtttcc ctcctatttc atttgttttt ctttccgtga catgtacaca ttagtggaga
    33481 tagctatagt tgcaacaatg ataaacaaaa ccgaagacag tgctgatgca tagcccaatt
    33541 cataaggtgc tgtaaatcct cgactgtaaa tatattgaac agctgtttct gtcttaaatt
    33601 gtgggccacc agcggttgca acatatactt ggtcaaacac ttgcatcgaa ccaatcagat
    33661 tcgttaaaat acaaaaacct aaggagggaa cgatctgagg tagcgtaatg ttaaaaaact
    33721 gctgtcgatt tgttgcacca tccatttgag cagattcata caaagaggga gagattccct
    33781 gaatagcagt caagagaatc gtcatagtaa caccaaaatt cttccaaaca gtcatgatgg
    33841 ctaccgtcgg catagcaagg gttggatcgc gaaaaaattg agggatcggc aagccgactt
    33901 gtgaaaacaa gtaaggaatc gcccctacat tcggatcaag caacatagaa aaaataatcg
    33961 caattgacgt caatgaacaa ataactggaa tgtaaaatgt cgaacgacaa aaacgattga
    34021 atctggtatt tttagataat gctactgctg ctaataaacc aacgataatt tgtgttggcg
    34081 tttcgagtac ggtgaaatat aaagaatgaa tcaacgaatt aactgctcgt tcatcactaa
    34141 atatacgtgt gaaattttca aaacttacaa aatttcttga tgtaaagaaa atattcaaat
    34201 ctgtaaaact aatagcaaat gttcctaaaa gcggtatgac agtaaataat aataaaacta
    34261 aaaaagatgg agttaaaaac aaatacgcag cacgatcagg acgattccaa tatttggtga
    34321 atccgttcat atagtccacc tcatctttca ttttgataaa atatacaatc ttggtttaaa
    34381 cgaatctgaa taagatatcc gaacaatcgc tgtgattttt gcaataacac ctatctgcaa
    34441 atactaggtc gatcattcag atatctgtat cttgccaaca gtctatcctg tataaagttc
    34501 tgtactattt ttgcagtaat tgattgatgg catcatctgc atcgttcatg agtgtctcag
    34561 gatcttctcc tgctaataat ttttcaatca gtggattgat gatatcgtta ttgatcgcag
    34621 agatttgggt aaaacctggt aaatatggct ctgcatagtc catttgtttc gataattcag
    34681 atacaatagg atcatttttt acttcttcat cttttgctac tgaatccaag taggcaggaa
    34741 aaccattttc gatactccat tttttaccaa tttcagttgt attccaatat ttcaaaaact
    34801 catagacagc ttctttttta ttattatctg tacttgccgg aatggcatat cctacgccat
    34861 ctaaaatagc tgtttttttg tctgaatcac tcccttgtgg taaggtagtc acattataat
    34921 caatctcgtt tgctttcaat ccgttattta accatggccc attgatttca atggctagtt
    34981 gaccagcatt cattaaattg tccatttcgg cgccagaaat cgattctgga ccagtcttgt
    35041 ctacatggat cattttttgg atcgctttta acgttttaag gttctcagga gacgcaaatt
    35101 cagacttagt ataatcatcg ttcactaatt taccgccatt tgctaacatc cagttatata
    35161 acggtgcagt tccatcctta ttgaagacaa aacctgatac atttttacta ctatcagtta
    35221 attttggtgc catttctgtc aattcttccc aagtagtagg tggttgttca gtatctaatc
    35281 cagcttgttt aaacaaatca ttattccaat acatatacat gccttgtacc tgcatgggga
    35341 caaaatactg tttaccttct acttcaccta attcaatagc cgtttcttta aaatcagatt
    35401 tatctactcc atcatattcc cagaaatcgt ctaatggttg taccgctccg ttttctacat
    35461 actgtgcaaa atctggataa tttaatgcaa taaagtcagg tgctttttta gaagaaatcg
    35521 ctgtaggaag tttttcattc aaattggccc aagtcatgat atccatttca attttgatgt
    35581 ttttatcatt tgttttatta aattcattaa cgatttcttg caatatttca ccatcagatg
    35641 cagtaaaccc attccaaaaa gtcaaagtca cttctttatc agaatcagat tctgctgaac
    35701 ttccgccacc gcaagctcct aaaatacctg ttgcagctac caacaaagag ccaattaacc
    35761 attttttacc tttcattttt tgttcctcct tcatctgata aacgacatct gaccacattc
    35821 caagatagcg gtttcaataa acaattgatt ttcccatctt cagcttttac atttgttgaa
    35881 tgacttggtt ttacttgttc acttttagga agattagttg cttttgtatc aaatccactc
    35941 atttcagaaa aatcaatcac tccctctaat gataattcag acaagtgaac agttagattc
    36001 atttcttctt ctccacggtt aactgcaaat atgacaagtt ctttgtcatt tattacggct
    36061 atactttcta aatacggcac ttcttcaaaa tcttccgtcg catagtttgc tgaacgaatt
    36121 tgtgggctta acacctctcc ttgtccatac acagcagctt gcataaatgg ataatagatc
    36181 gtctgtctcc aagcttctcc gtttgtttct gtcctgattg gtgcaataac gtttaccaat
    36241 tgagcaagac aagcaatctt tactctatct gcatgtttca ataaagtgat taatagacag
    36301 cctaatagta atgcatcttc aaaattatag ttatcttcta gtaaaggagg agcaatctgc
    36361 caaggcgctg cttgctcatc ttgttccgct gaatgatacc aaatattcca ttcatcaaag
    36421 gacaggttga tctgcttctt actatgtttc ttcgccttga ctgtatcgca catcgctgtc
    36481 acttcagaaa tgaaccgatc catgtctaag gaacgtgcaa gataatgaga aagatcattt
    36541 ttttggttgc cataatattg atgcagagac aaataatcta tttgatcgta cgcttgatca
    36601 agaactgtta actcccaatc accaaatgta ggcatttgcc gattcgaact tccgcataaa
    36661 accacctcaa ttgaatcatc tacgagtttc atcgccttag ccgtttcctc tgctaaccgt
    36721 ccatattctt ccgctgtttt atgtccaatc tgccaaggac cgtccatttc attccctaaa
    36781 caccatgtct taattccaaa agggctctct tgaccattcg aacgccttaa atcactccaa
    36841 taagttcctt taggaaaatt acaatattcg actaaattac gagctgcatc tactccacga
    36901 gtacccagat tgacagccat attgatttcg gcccccacat gctgacacca gttataaaat
    36961 tcatgaatcc caacttcatt cgtttcaatt gtccgccaag ctaaatccaa ccttctaggt
    37021 ctatttgcta tgggcccaat actgtcttcc caacgaaatc ctgacacaaa atttccacca
    37081 ggatatctaa tcatgggaat gttcaattct tttaccaagt gaatgacgtc ttggcgaaat
    37141 ccacttttat tagctgatgg atgatctggc tgataaatcc cttcatatac tgctcgtccc
    37201 atatgttcaa taaatgaact gtacaaccgc ggatcaactt tgcctacacg cttgacacta
    37261 ctaatcaaca ttgtggcttc cataactgac ctcctttaat ttatacatga ataatacacc
    37321 tcgttgaaag cgttgtcaat aatataaatg ttaaacttac ttatttttta tcgattttaa
    37381 ttaaccaaaa tattaataat gattaaaaca aataatttaa tataactttt attaacaaat
    37441 atgttaatat ataagaaaaa ccttttaaga ggtgatatat atgcaactac aaataaacga
    37501 agaatcctta ccggtatatg aagcattagc tagtaaaaca aggatcaaga tcatccagct
    37561 actttccaaa aaaaagatga atgtcaaaga tttggcaaaa gaacttggag tgagcagtgc
    37621 tatcacgacg atgcacgtga aaaagctaga ggaagcaaac atcatcaaaa cagaaaaagt
    37681 aggacaacaa aaaatttcga gccttcgtgt cgataaaatc gatattagtt ttccagaaaa
    37741 aatattcaat gcttttgata cgaaagaaac atcgatacct attggtcact atacaaatta
    37801 tgcaattgaa ccaacatgcg gtttagctac gatccatgat tttatcggga aagtggatga
    37861 accacgctat tttatggatc ctcgaaggat ggatgcccgc attctctggt ttaccagcgg
    37921 atttgtcgag tatcaggcac ctaatttttt aaatacagag gatacattgg agatgttgga
    37981 agtttctgtg gaaatttctt ctgaatttcc tttttcaaat gacaattggc cttcagatat
    38041 cactttcagc ctaaatggtg tggagctagg aacatggacg agccctggag attttgctga
    38101 tatccgcggt aaatatacgc ctgattggta tccagataat cttaatcaat acggcttgct
    38161 gaagacgatc cgaattacca aacacttgac gaatatgaat ggagaaccct tgtcaaacat
    38221 cacgatcaac gacatcccta aagagcaaga cacttggcat ttgcgaatcg aagtgaaaga
    38281 cgatgccaaa catgtggggg gctgtacttt atttggtaaa ggattcggta attacgatca
    38341 ggatatcaaa ttaaaagttt actacagctg atatttttct tttctatttt ataaaacaaa
    38401 aacagccaat ccctatgaag ggacggctgt ttttgcttta ttgttatttc cagaaatcat
    38461 ccaaaacgga aattggcaaa tgtcgtttat gttgtgtctt gttccaccat gcttcgattt
    38521 tttcttgtgc ttgagcggga acttcttttc cttctagata atcatcaatt tcattatatg
    38581 tgacacctag tgcgacttca tctgcaatca ttggtttccc atcttcaaga tctgcagtcg
    38641 gaatttttgt gtataatttt tccggtgcat tcaatgcttg aagcaattgt ttcccttgtc
    38701 gtttgtccaa acggaataat ggcaaaatgt cggctccacc atcaccaaat ttcgtgaaga
    38761 atccagtaat gttttctgca gcatgatctg tccctaatac ggctcctgct ctttctccag
    38821 ctacggcata ttgcgtaatc atacgttgac gtgctttgat atttcctttg ttaaaatcgc
    38881 tgattcccac acctgcttcg cttaatactt gtacttgtgc atcaacagct ggtttgatat
    38941 ttactcgcag actaacatcg ggctggataa agtcaagagc agcttttgca tcttcttcat
    39001 ctgcttgttc accataagga agacgaacag cgataaattg atagttttca tcttttgttt
    39061 cttcacgcat ttcttccatc gttagttgcg ccaaacggcc tgctaaagta gaatcttggc
    39121 cgccgctgat acctagtaca aaagttttaa gaaacggata ttttttcaaa tatgctttca
    39181 taaagtcaat acttttacgt atttcttctt ttggatcaat tgttggtttg acacctaatt
    39241 cattgataat ttctgtctgc aggctcattt attctcctcc tatgcttctg tatgaagttc
    39301 tgctacgctt tttttcattc gttccatgat agccatttta tggttccagc attcagtaga
    39361 cagatcgact gggtattttt gcggattcaa gtctcgttta tactcttccc atagtgaatc
    39421 tagattttct tttgtatatt ctttgatttc gtttagtgca ggtaattcgt aaactctttt
    39481 tccttcgata aagatatctt gtaaaactgg gcgagctgta aagtctcgaa cagttttatt
    39541 gataaacgta tgcactggat ggaacatgaa gatagcttct tcttctcgag ggtcttcatc
    39601 ccataatgtc acgtagtctc cttcagattt cccatcaaaa tttcgagtga tgcgccacac
    39661 ttgttttttc cctggagtag ttactttttc tgcattgctt gataacttga tcgtatccat
    39721 catttttcct tcatcatctt cgatcgatac tagcttgaat actgcaccaa gggctggctg
    39781 atcataggct gtgatcaatt ttgtcccaac accccataca tcaattttag ctttttgcat
    39841 ctttaaattc aaaatcgttg cttcatccaa gtcattcgaa gcatagattt ttgcttccgt
    39901 aaatccggct gcatctaatt gttcccgaac acgcttagat atataggcca tatctccact
    39961 gtcgatgcgt acgccttgga agttgatttt gtcacctaat tctttcgcca cgcgaatcgc
    40021 acttggcaca cctgatttca acgtatcata agtgtcgact aagaacacgc agtctttatg
    40081 ggttttagcg taggccataa atgcttcata atcatttcca taagattgga ctaacgaatg
    40141 agcatgggtc ccgctagctg gtatcccaaa tatcttccca gcacgtacat tgcttgttgc
    40201 atctgctccg ccgatatatg ccgctctagt tccccagact gctgcatcca gttcttgtgc
    40261 ccgtctagtt ccaaattcca atagtggatc ttctccgatg acagatttga tacgagctgc
    40321 ttttgtcgcg atcaacgttt ggaaattgac catattcaac agtgctgttt ctactaattg
    40381 gcagtgcgct aaaggccctt ccacttggat caatggttca tttgcaaaaa ctagttctcc
    40441 ttcacgagca gaacgaactg ttgctttaaa ttcaaaattt tgtaaatact ccaaaaaacc
    40501 ttcaggatag acttctaatc cacgcaagta agcaatatcg gaatcattga acgtcagatt
    40561 ctctaaataa ttgactaacc gttccaatcc tgcaaaaacc gcgtatccgt ggttaaaagg
    40621 catttctcga aagtaacatt caaaaacagc atgtaaatca gctcgaccta attcccagta
    40681 tgtctgcatc atattgattt gatatagatc cgtatgcagc gtcaaactgt cgtctgggta
    40741 agtattattc atcttgtatt actcctcttg gcaagttttc atttcctacc cattataaca
    40801 aaaaaaatta ataggaaaag aaatcttcca ttttttctga aaaagcacat gttttacgga
    40861 tatttcttgc taaaaatgaa ctccatctgc taaaactatc ctttcttatt ggtatttaac
    40921 ctaaacgaga aagaatactt attttattca atgtatccga gacacgaaga aattagcgtt
    40981 ttctattttt cttacttgat aaaataagta gattttccta ctacaatcgt agccgttaag
    41041 ataccgatga ttgaacaaat ataaccaaat gtctctacaa cataatcaat ggcaaaagtt
    41101 tttgaagcaa taatacgtct ttcctttttt gcttcttatc cccaaagaaa cggatataac
    41161 ttattatgta ttgaattttt ttggaaaggc gaattatgac aagatatacc ttgcgttgaa
    41221 aaaactcgcg gaaaaaatat cacacgtttt taaaaagata caaagtaaga aagcagctgt
    41281 caatagaatg gaaacaaaaa tggcgagaca tcaatcagtc tcgccttgtt ttt
//
LOCUS       DAZUKR010000011        40550 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000011 DAZUKR010000000
VERSION     DAZUKR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.011"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACG89C_000537"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACG89C_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4899116.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACG89C_000538"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACG89C_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4899117.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG89C_000539"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG89C_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4899118.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG89C_000540"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG89C_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4899119.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACG89C_000541"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACG89C_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4899120.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACG89C_000542"
     CDS             7565..8422
                     /locus_tag="ACG89C_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4899121.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACG89C_000543"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACG89C_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS4899122.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACG89C_000544"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACG89C_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4899123.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACG89C_000545"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACG89C_000545"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4899124.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACG89C_000546"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACG89C_000546"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4899125.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACG89C_000547"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACG89C_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4899126.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACG89C_000548"
     CDS             13203..13895
                     /locus_tag="ACG89C_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4899127.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACG89C_000549"
     CDS             13892..14659
                     /locus_tag="ACG89C_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4899128.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACG89C_000550"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACG89C_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS4899129.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACG89C_000551"
     CDS             16624..16944
                     /locus_tag="ACG89C_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS4899130.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACG89C_000552"
     CDS             16944..17294
                     /locus_tag="ACG89C_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4899131.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACG89C_000553"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACG89C_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS4899132.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACG89C_000554"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACG89C_000554"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4899133.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACG89C_000555"
     CDS             19288..20313
                     /locus_tag="ACG89C_000555"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4899134.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACG89C_000556"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACG89C_000556"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4899135.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACG89C_000557"
     CDS             21391..21831
                     /locus_tag="ACG89C_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS4899136.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACG89C_000558"
     CDS             21834..22436
                     /locus_tag="ACG89C_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4899137.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACG89C_000559"
     CDS             complement(22495..23601)
                     /locus_tag="ACG89C_000559"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS4899138.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG89C_000560"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG89C_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS4899139.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="ACG89C_000561"
     CDS             complement(25149..27494)
                     /locus_tag="ACG89C_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS4899140.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="ACG89C_000562"
     CDS             27773..28675
                     /locus_tag="ACG89C_000562"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4899141.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="ACG89C_000563"
     CDS             28888..30663
                     /locus_tag="ACG89C_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS4899142.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="ACG89C_000564"
     CDS             complement(30729..32315)
                     /locus_tag="ACG89C_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4899143.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="ACG89C_000565"
     CDS             32566..32790
                     /locus_tag="ACG89C_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS4899144.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="ACG89C_000566"
     CDS             32932..34683
                     /locus_tag="ACG89C_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4899145.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="ACG89C_000567"
     CDS             34683..36467
                     /locus_tag="ACG89C_000567"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899146.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /gene="licT"
                     /locus_tag="ACG89C_000568"
     CDS             36793..37644
                     /gene="licT"
                     /locus_tag="ACG89C_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4899147.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37737..39623
                     /locus_tag="ACG89C_000569"
                     /pseudo
     CDS             37737..39623
                     /locus_tag="ACG89C_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(39663..>40550)
                     /locus_tag="ACG89C_000570"
     CDS             complement(39663..>40550)
                     /locus_tag="ACG89C_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4899148.1"
                     /translation="KIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYVHPSTL
                     IRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMASLEQM
                     TIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDVTIVTT
                     FGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDSTLSAMS
                     TAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQKFDKR
                     PISSAIMKEPFNFENKEVE"
BASE COUNT        13042 a         7167 c         8632 g        11709 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt
//
LOCUS       DAZUKR010000012        38768 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000012 DAZUKR010000000
VERSION     DAZUKR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.012"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..1002
                     /locus_tag="ACG89C_000571"
     CDS             64..1002
                     /locus_tag="ACG89C_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4899149.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            1025..1705
                     /locus_tag="ACG89C_000572"
     CDS             1025..1705
                     /locus_tag="ACG89C_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4899150.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            1678..2502
                     /locus_tag="ACG89C_000573"
     CDS             1678..2502
                     /locus_tag="ACG89C_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4899151.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            2669..3487
                     /gene="purR"
                     /locus_tag="ACG89C_000574"
     CDS             2669..3487
                     /gene="purR"
                     /locus_tag="ACG89C_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS4899152.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            3990..5363
                     /gene="glmU"
                     /locus_tag="ACG89C_000575"
     CDS             3990..5363
                     /gene="glmU"
                     /locus_tag="ACG89C_000575"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4899153.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            5457..6428
                     /locus_tag="ACG89C_000576"
     CDS             5457..6428
                     /locus_tag="ACG89C_000576"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4899154.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      6434..6536
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6602..7171
                     /gene="thiT"
                     /locus_tag="ACG89C_000577"
     CDS             6602..7171
                     /gene="thiT"
                     /locus_tag="ACG89C_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS4899155.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(7252..8025)
                     /locus_tag="ACG89C_000578"
     CDS             complement(7252..8025)
                     /locus_tag="ACG89C_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899156.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            8292..9992
                     /locus_tag="ACG89C_000579"
     CDS             8292..9992
                     /locus_tag="ACG89C_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4899157.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            9962..10180
                     /locus_tag="ACG89C_000580"
     CDS             9962..10180
                     /locus_tag="ACG89C_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS4899158.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            10200..11090
                     /locus_tag="ACG89C_000581"
     CDS             10200..11090
                     /locus_tag="ACG89C_000581"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4899159.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            11108..11392
                     /locus_tag="ACG89C_000582"
     CDS             11108..11392
                     /locus_tag="ACG89C_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899160.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            11879..12493
                     /gene="gmk"
                     /locus_tag="ACG89C_000583"
     CDS             11879..12493
                     /gene="gmk"
                     /locus_tag="ACG89C_000583"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4899161.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            12498..12803
                     /gene="rpoZ"
                     /locus_tag="ACG89C_000584"
     CDS             12498..12803
                     /gene="rpoZ"
                     /locus_tag="ACG89C_000584"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4899162.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            13056..15464
                     /gene="priA"
                     /locus_tag="ACG89C_000585"
     CDS             13056..15464
                     /gene="priA"
                     /locus_tag="ACG89C_000585"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4899163.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            15479..15970
                     /gene="def"
                     /locus_tag="ACG89C_000586"
     CDS             15479..15970
                     /gene="def"
                     /locus_tag="ACG89C_000586"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4899164.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            15963..16901
                     /gene="fmt"
                     /locus_tag="ACG89C_000587"
     CDS             15963..16901
                     /gene="fmt"
                     /locus_tag="ACG89C_000587"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4899165.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            16901..18259
                     /gene="rsmB"
                     /locus_tag="ACG89C_000588"
     CDS             16901..18259
                     /gene="rsmB"
                     /locus_tag="ACG89C_000588"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4899166.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            18272..19012
                     /locus_tag="ACG89C_000589"
     CDS             18272..19012
                     /locus_tag="ACG89C_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS4899167.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            19009..21078
                     /gene="pknB"
                     /locus_tag="ACG89C_000590"
     CDS             19009..21078
                     /gene="pknB"
                     /locus_tag="ACG89C_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS4899168.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            21243..22142
                     /gene="rsgA"
                     /locus_tag="ACG89C_000591"
     CDS             21243..22142
                     /gene="rsgA"
                     /locus_tag="ACG89C_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS4899169.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            22155..22805
                     /gene="rpe"
                     /locus_tag="ACG89C_000592"
     CDS             22155..22805
                     /gene="rpe"
                     /locus_tag="ACG89C_000592"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4899170.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            22806..23444
                     /locus_tag="ACG89C_000593"
     CDS             22806..23444
                     /locus_tag="ACG89C_000593"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS4899171.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            23599..24453
                     /gene="mreC"
                     /locus_tag="ACG89C_000594"
     CDS             23599..24453
                     /gene="mreC"
                     /locus_tag="ACG89C_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4899172.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            24457..24966
                     /gene="mreD"
                     /locus_tag="ACG89C_000595"
     CDS             24457..24966
                     /gene="mreD"
                     /locus_tag="ACG89C_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4899173.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            25221..26795
                     /locus_tag="ACG89C_000596"
     CDS             25221..26795
                     /locus_tag="ACG89C_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4899174.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(26863..28662)
                     /locus_tag="ACG89C_000597"
     CDS             complement(26863..28662)
                     /locus_tag="ACG89C_000597"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS4899175.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            28785..29291
                     /locus_tag="ACG89C_000598"
     CDS             28785..29291
                     /locus_tag="ACG89C_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4899176.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            29370..30092
                     /locus_tag="ACG89C_000599"
     CDS             29370..30092
                     /locus_tag="ACG89C_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4899177.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(30170..31570)
                     /locus_tag="ACG89C_000600"
     CDS             complement(30170..31570)
                     /locus_tag="ACG89C_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS4899178.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(31740..32714)
                     /locus_tag="ACG89C_000601"
     CDS             complement(31740..32714)
                     /locus_tag="ACG89C_000601"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4899179.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            33067..33627
                     /gene="pth"
                     /locus_tag="ACG89C_000602"
     CDS             33067..33627
                     /gene="pth"
                     /locus_tag="ACG89C_000602"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4899180.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            33644..37165
                     /gene="mfd"
                     /locus_tag="ACG89C_000603"
     CDS             33644..37165
                     /gene="mfd"
                     /locus_tag="ACG89C_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4899181.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            37180..>38768
                     /locus_tag="ACG89C_000604"
     CDS             37180..>38768
                     /locus_tag="ACG89C_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4899182.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
BASE COUNT        12549 a         7060 c         8351 g        10808 t
ORIGIN      
        1 gatacattag ttcacgttaa taaatcgtaa aattccgatt tgttcattga aaggagcata
       61 taaatgaaaa aatatctttt gacagctacg gtaatgatag gtgctttact gtttgcagca
      121 tgtggaaata cgaacaagga agcagacaaa aaagaggatt tgacgatcgt tacgactttt
      181 tatccgatgt atgattttac aaaagagatc gtaggggatg aaggaaacgt gaaacttttg
      241 atccctgctg gaacggagcc gcatgatttc gaaccaagtg caaaagaaag agcagaaatt
      301 tccgatgcag atgttttcgt gtataatagt tcagacatgg aattttttgt ggattcgtta
      361 aaggattcag tggatagcaa acagaccttg atgatcgaag cagctaaagg aatcgaccgt
      421 ttagaatcgc aagaagcaga cgaacatgaa gaatcagaag aagggcatgg tcattcacat
      481 gaatacgatc cacatgtttg gctcgatccg gtattagcta taaaagaagt cagaacgatt
      541 gctgaagaat taggcgaaaa atatccagat aaaaaggaaa cttttacgaa gaatgccgat
      601 gcttatatca aaaagcttga agcactggat caaaaatact ccgatgagct aaaagatgca
      661 acaaaccgca catttgtcac tcagcatgct gcttttgctt atttagcgaa tcaatacgac
      721 ttaaaacaag tagcgatttc gggcgtttca ccagatcagg aacccacacc aagtcgctta
      781 gctgagctaa aagaatttgt gaagaaaaac aacatcaaag tgatttattt tgaagaaaat
      841 gcctcttcga aggtagctga gacattgtcg aatgaaacag gagtgaagct agaagtgttg
      901 aatcctttag aaagcttgac taatgaacaa atcaaagcag gcgaaaatta tatttctgtc
      961 atggaaaaaa atctgaaagc tttaaaagaa agtatcaatt aagttttaga atggaggttg
     1021 taaggtgcgt tatatcgaaa tatctgattt gaccttttac tatgatgatg aacctgtact
     1081 agaagatgtc tcttatcatg tagatgcggg tgaatttgtc attttaactg gagaaaatgg
     1141 agcagccaag tcaaccttgg tgaaagcatc gttaggatta ctgaaacctt caaaaggaat
     1201 gataaagatt gctgagaaaa atcaagaagg aaaaaaatta agtatcggtt atattcctca
     1261 acaagttgca tctttcaatg caggttttcc tagtacggtg atcgaattag tccgttcagg
     1321 aagatatcca agaggccgct ggttcaagcg tctctctgaa aaagatcatc aacatgtaca
     1381 gcgtgcttta gaagctgtag ggatgtggga tatgcgaaat cggcgaatag gtgaattgtc
     1441 tggcggacaa aagcagcgaa tcagtttagc gcgtattttt gctactgacc ctgacttatt
     1501 tgttcttgat gaaccaacaa caggtatgga tgaagaatcc agaaatgaat tttatcgtct
     1561 tctccgacat agtgcgcatg tccatgggaa atcagttcta atgatcaccc atgatcatga
     1621 agatatcaaa cagtatgcgg acagacagat ccgactggtg agaaaggagg attctcaatg
     1681 gcgttgcttt catatgaatt catgagaaga gcgtttcttg ccgctatctt cattgccgga
     1741 atcgccccga tgctaggtgt gtttttagtg atcagaagac aatcgctgat ggctgatacg
     1801 ctttctcatg tctctttagc aggggtagcg ttaggattct ttttgaacct taatcctaca
     1861 cttacgacca tgctagtcgt tgtattagca gctgtgatat tggagtatct gcgaacgatc
     1921 taccgaacct attcagaaat ctccattgcc attttaatgg ctggtggttt ggcgctagcg
     1981 ttagtattga tgaatttgag cggagggaat tcagcaacaa gtatccaatc ctatctgttt
     2041 ggttcgatcg tgaccattac atgggagcaa gtcatctttt taggaatctt attcgttgcc
     2101 atcggtatat tgttcttttt attcaaacgg ccgatgtatg ttctgacttt tgatgaagat
     2161 accgcccacg tggatgggct acccgttcat ttgatgtcga tgatgttcaa tgtgattact
     2221 ggtgtggcta ttgctgtgat gatcccgatt gccggtgcac ttttgatatc cgcaatcatg
     2281 gttcttcccg cagcaatcgg tatgcgatta ggcaaaagct tcaatgtcgt gattgctatc
     2341 agtgtttgtg taggatttct agggatgctg tcaggactaa ctagctcgtt ttatctagat
     2401 acacctccag gagcaacaat cacattagtc tttattgctt tgttcctgat tgtaaatatc
     2461 atcaaacgaa tattggttgc cttcagaaga aaaaataact aaattataaa gaaaagaagc
     2521 cttataaaaa cgaaaaattt tttataggct tctttttatt ttaataaata ccgaactatt
     2581 ttcttatttt ttcataaaat gctcgaattt ttgaagaaaa acttatataa tgtaaaacga
     2641 taaaatttct taaaaggagt tggaataggt gaaagtacgt cgcagtgaac gtctagttga
     2701 catgacacag tatttattga atcacccgca tgaattgatt tccttaacaa gctttgccga
     2761 acgttatgaa tctgctaaat cttctatcag tgaagatttg ggaatcatca agaaaacatt
     2821 taaagaacga gggtatggaa ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt
     2881 catccctgaa atttcatatg aagaggcgaa aaaatatatt gaatctctag cagaacgtct
     2941 atcagaacaa gaccgtctgc ttccaggagg atatgtttat ctctctgatt tgttaggaga
     3001 acctgaactg ctgcgtcaag ttggaagaat cattgcttct aaataccttg gtgaaaaaat
     3061 cgatgctgta atgactgtag ccacaaaagg cgtacctatt gcccaagctg tgtcctatta
     3121 tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa ataacagaag gatcaacagt
     3181 aagtgtcaac tatgtgtctg gatcttctga aagaattgaa aagatggaac tgtctaagag
     3241 aagtttgaaa cgtggttcac gtgtccttgt agtggatgat ttcatgaaag gcggcggaac
     3301 agtcaacgga atgaagagct tgattgaaga attcgaatca gaacttgttg gcatcactgt
     3361 atttgccgaa tcaaaattca acggtcatcg cgccatcgat gattatacgt cactgcttta
     3421 tgtcaaagat gtagatactc aaacgaaaaa tattacggtt gtgccaggaa attatttttc
     3481 agaataagag gttgtgaaaa agccgcttag tttcgagtag taagaaaaat tttgtgaaaa
     3541 tggctttcca cattttttca taaatttggc ttaataccga gaaactggct tttgaacacc
     3601 gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa taaacggaaa
     3661 aattgctcct catattttgc cgtttattga gctaataccg aaggtcagct gattgaaaaa
     3721 cgtttaatga agaggttgta agcctaccgt ttaactctaa gtcacaagaa acgatttcaa
     3781 aaaacagctt ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt ggcaggagag
     3841 caccggttac caaatagata ggcccgttat ggtctatcta tttttttatc cagaaaacgc
     3901 tgggataaaa gaaaatcact tgttttgttg aaaaaatggg tatactggga taaactgaaa
     3961 tcagcaaact atatttagga gtgacgcagt tggacgcacg ttatgcaatt attttggcgg
     4021 cagggaaagg tacccgcatg aagtcaaaat tatataaagt tttacatccc gtttctggta
     4081 aacctatggt agaacatatc attaatcggg tcagtgaaac gaaaccagat gaagtgatta
     4141 cgatcgttgg acacggagca gaacaagtta aagcacaact aggagaacgc agtaaatatg
     4201 cgttacaagc agaacaattg ggaacaggac atgctgtttt gcaagctgct tctttcttgg
     4261 aaggaaaaaa aggaacaacg ttagttatca gtggcgatac accattattg actactgaaa
     4321 cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg
     4381 cacaagcaga aaacccaaca ggatatggac gaatcatccg tgaccatatt gggatcgtag
     4441 aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa gaaatcaata
     4501 caggaactta ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa
     4561 acaatgctca aggtgaatac tatctaacag atatcatcga aatcttaaaa gaagaaggcc
     4621 atacagttgc tgcttatcaa acggatgatt ttgaagaatc aatgggagtc aatgatcgga
     4681 tcgcgttagc taaagcaaat gagatcatga gaaagcgaat taaccagatg catatggtca
     4741 atggcgtaag ctttgtcgat tcagcaacga catacatcga tgcaggcgta gaaatcggtc
     4801 cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt
     4861 gtgtcatcgg gtcacattca aaaatcgtag acagtaggat tgaggatcac gtcgtcattg
     4921 aaaactcagt cattgaaagc agccatgtca agaaacatgc agatgtggga ccttatgcac
     4981 atttacgacc aaaagctgaa attggcgaaa acgtccatat cggaaacttt gtggaagtga
     5041 aaaatgctca aatcggcaaa ggaactaaag tcggtcactt gacttatgtt ggtgatgcta
     5101 cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga
     5161 acaaacatca tacgactgtc ggcgaccaca gctttattgg ttctagcacg aatatcatcg
     5221 gacctgtcga ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt acagacaata
     5281 ttccagaata tgcgctggca attgcgagag caagacaagt caataaagaa ggatatgcaa
     5341 aaaaactccc ttatctaaac taagactttt gcaaaatcac ttgatttatt taaaaaatat
     5401 gactactatt tattaggtag aattctagag ttgtgaaaaa gaaagtggag gtccccatgt
     5461 caaaacatta ctttgaccca agattaaaaa tttttgcgtt aaattccaat cgcccattag
     5521 cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt actcaattta
     5581 gtgatggaga aattcaagta aacattgaag aaagtattcg tggatcgcat gtctatgtta
     5641 ttcaatcaac gagcagccca gtcaatgata acttgatgga gttgctgatc atgattgacg
     5701 cactgaaacg agcaagtgca aaaacaatca acgtagtcat gccttattat ggctatgcgc
     5761 gtcaagaccg taaagcacgt gcacgggaac caatcacagc gaaattagta gcaaacatga
     5821 ttcaaaaagc tggtgcaaca agaatgctga ctttggacct tcatgcagtc caaatccaag
     5881 gtttctttga tattccagtc gatcatttga tgggtgcgcc acttattgcc aattatttct
     5941 tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc accagaccat ggtggtgtca
     6001 cacgggcacg taaattagcg gaatttttga aatctccgat tgcgatcata gacaaacgcc
     6061 gtccaaaagc aaatgttgca gaagtaatga atatcattgg acaagtcaaa ggcaaaactt
     6121 gtgtactgat cgatgatatg atcgatacag caggtacgat cacattggct gccaatgctt
     6181 tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac acatccagta ttatccggcc
     6241 ctgctttaca aagaatagaa gattcagcta tcgagcgtct agttgttaca gactcgatct
     6301 atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg
     6361 gagatgcaat caaacgtatc catgaaaaca aaccagttag tccattgttt gaaacaaaaa
     6421 ataaataata aataaactca caaaggggag tccaattggg ctgagattga atctatttct
     6481 aaacccttcg tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg
     6541 actattttcg aaagctgctt cctcctttgt gactttttta ttcaaaggag gaagtttttt
     6601 tatgggaaaa aatagagttt gggtagaagg aacgattgtt gcagcgttag cgatggtgtt
     6661 gtcgttgatt ccgatccaga tcggcagcag tttttctatt tcattagggc agattccatt
     6721 gactcttttt gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt tcatttgggg
     6781 catcctacat tttccgttgg gacaagttta ttatttaagt gtcgttcaag tgttgattga
     6841 gtacccgatt gcctatactt ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa
     6901 aggctatgta gacgataata cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat
     6961 tggcgcactt tccagatatt tttggcattt tattgcagga gtggttttct ggggagcgta
     7021 tgcactatgg gggatgaatc cttggctctt ttctttcgta atgaatggtg ccagcgggtt
     7081 gagtacagct attgtaacga tcatcgtttt attagggatc caacaaacga ctccttcgat
     7141 ttttgtgcca gataaacgac aggagctgta aataaaaaag aagcaagaaa cttgagacag
     7201 aatcatgtcc tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa
     7261 tggcagataa gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta
     7321 gattaaagta gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata
     7381 atcttcaatc acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatcaaa
     7441 agaaaaaatg atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat
     7501 tttttcaaca ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa
     7561 gatcgaactt aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat
     7621 ccgattcagc tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa
     7681 cggcgaatga agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg
     7741 tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc
     7801 acgcctgtta cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt
     7861 cagccctgta tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt
     7921 ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac
     7981 agcattactt gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt
     8041 atttcctctt aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat
     8101 tttaaaattc cattacaaaa tcacgttttc attgaaaaca ctctcatttt caattaattt
     8161 ttgtgatttt tgtatttttt tatttaaagg aggttttatg aattgttttc ttatggaatc
     8221 tacaaagtcg aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa
     8281 aggagagcgc gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc
     8341 ctatccagca agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg
     8401 ctttttggcg ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg
     8461 tctattttac tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag
     8521 gtgaaacttt tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta
     8581 ttaaagaaaa ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt
     8641 ttcataaaaa cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag
     8701 cacttgtttt aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc
     8761 tgaaggaaca tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg
     8821 gcgcattgat tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga
     8881 caagacaagt gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct
     8941 atcgagaaag acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc
     9001 cattggaaaa gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa
     9061 tacgtttata tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc
     9121 aacagacaaa aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg
     9181 ctatggctta tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa
     9241 tacctattga tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag
     9301 ggccaggccc agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa
     9361 cattgtggac actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac
     9421 aattaggcta tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat
     9481 ttcattctgt taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag
     9541 aaattcagtt agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt
     9601 tatataagaa cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa
     9661 aaaaacattt tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct
     9721 gccaacaaga tataaaaagt atgccggatg atttattggc acaaattggt caattaagta
     9781 ctgaagaatg gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa
     9841 ctgtttattt tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc
     9901 ttaagcaacc agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac
     9961 catggcacaa gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa
    10021 caaggggtgg cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga
    10081 tcttcgagat tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga
    10141 agagtttgaa caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa
    10201 tgaaaagtat gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata
    10261 ttgaaataaa aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc
    10321 tgaactttgt tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa
    10381 tcgaagtatt tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact
    10441 gggaattgat tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta
    10501 gtcaaaatca agtgtcaatt agtcggatgg cggaacgctt cgtgctggac gaaaacttcg
    10561 taacggtaga agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata
    10621 cattgaaaag cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag
    10681 ctttagtgtt gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt
    10741 ttgtagaaat ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc
    10801 ttggagcgaa agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg
    10861 gagatattca cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta
    10921 tcgaagcaca atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg
    10981 aaatcaacac gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt
    11041 ctaagacgat cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt
    11101 agagataatg aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa
    11161 acgggatatc ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta
    11221 taatgagaac gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa
    11281 atttattgtt cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg
    11341 aaaagatgag atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg
    11401 ctgaacaaag aaatacaata aataaacaga attggatttc ttcgcttagc ttctttgctc
    11461 taggaataca tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata
    11521 agtgatgttc aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa atgtgagaaa
    11581 ctattttcac aaaatttttc ttcatactcg aaactaatcg gcttcttcac gacctcttat
    11641 acacggcgtt caaaagcttg catctgcgat aataacccca accaaacgaa aaacatgaga
    11701 agctgtttat gtttggctgt tcttattact tgatgcagga cagctttttt acgacctctt
    11761 ggtacagggt agctttttta ccaatttcta gctataatta aagataacct tgaaagttgc
    11821 agaaaaaaag tgcaaaataa acgataagta caaaaagact agttgaaagg acgtcgtcat
    11881 gtcagagcgt ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa aaggcacagt
    11941 acgaaaagca atttttgaga gtgaggataa tgattttcag tattcgatct caatgactac
    12001 ccgtaaaatg cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa gcaaagaaga
    12061 gtttgaatca atgatcgaag ctggagagat gctggaatac gcagaatatg tcgggaatta
    12121 ttatggaact cctttgtcat atgtcaacaa gacattggat gaaggaaaag atgttttctt
    12181 ggagattgaa gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg gtgtattcat
    12241 ctttttgact ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc gaggaacaga
    12301 tgcagatgaa gtaatcgaaa aacgaatgaa agttgcaaaa gaagaaatcg aaatgatggc
    12361 tctttacgac tatgctgttg taaatgatga agtgccgcta gcagttgaac gaatcaaaaa
    12421 cattattgcc agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata ttaaaatgtt
    12481 aaaggagatg tagtcttatg atgttaaaac cttcaattga ctcactatta gatcgtgtaa
    12541 attcaaaata ttctctagtg attttagcaa gtaaacgcgc gcatgaattg gatgcaagtg
    12601 cacagcctac tctagattct tttgattcag taaaaagcgt cggtcaagct ttagaagaaa
    12661 tcgatgcagg aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa cgtttgaaga
    12721 tggaagaaga ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg gaatcacgta
    12781 tccgtgaaga gcaaaaaatg taataaagaa aaatagagcg aacatctaaa aagaaaagca
    12841 aacccagctg tttccttttc aaacattcgc cacttattgt ctggtagaaa aggctgtgtc
    12901 tgaacttttt gacacagttc ttttttttct tatctgctgt taattttttg ttaggatttg
    12961 gcagaagata agacaaatcc atcgatctct agtaaaatag gcaaggaact ttttgtcgct
    13021 tgataagaaa agacaaaaaa aggaggaaac gaaccatgac attaacagct gaagtcattg
    13081 tggacgtacc tactatgcag accgatcagc cattcactta tttggttccc tctgaaatgg
    13141 aaacagcttt gcaagtcggg atgcgagtag aagtcccttt tggtaatggc aatcgtcatg
    13201 tccaaggatt tgttatggct atccaaaaat cagaagaatc agctaatcca tctttgaagg
    13261 cagttattcg tttgctagac cttgctccgg tggtcaatga agaattactt tcattagctg
    13321 attatatgaa aaaaattact tacgcattca aaatcacttg tctacaaacg atgcttccga
    13381 gtgtgatgaa agcagaatat gataaattga tctatccttt ggcagacaca ccagaagttc
    13441 aggtattgtt tgatcagcga gaagtgattt cctggaagga agcagaagaa gaaggcagcc
    13501 tttctcaatt gattcgctgg cgccaagagc aattagtaga tatcaaatat gaagtccata
    13561 cacgaaataa agtaaaaacg attcgtttgg tccgttcttt gttaactgaa aagcaaatcg
    13621 aagaagaatg ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt cttttgtgtt
    13681 tatctgaact gagccaagaa gaaccaattg cttatttcaa aaataaagga atcagtactg
    13741 ctgtcttgaa tcaaggaaaa gaaaagggct ggctggagtt tatagaatca gaacgttatc
    13801 gcgaccctta taaagaccga gtatttgacc aaacgacggc attggaactg aatgcagaac
    13861 aaaaaaatgc tgtcgaacaa atcatcacag caggtcagca gcaaaaagat caggtctttt
    13921 tgctggaggg aatcactggc agcgggaaaa cagaagtata tcttcaggca atcgctgatg
    13981 ttttgtcaga aaataaaacg gctattatgt tagtgcctga aatcgcattg actccgcaaa
    14041 tggtcgagcg attcaaagga cgcttcggag aatcagttgc ggtcttacat agcggacttt
    14101 cccaaggtga aaaatatgat gaatggcgca agattgagcg ggaagaagcg caagtagtag
    14161 taggcgcacg atctgctatt tttgccccgc tgaaaaatat cgggctgatc attatcgatg
    14221 aagaacatga atcaagttac aaacaagatg agacaccgcg ttatcatgcg agagatctag
    14281 caatttggcg cagtaaatat catcattgcc caatagttct aggaagcgcg acgccctctt
    14341 tagaatcaag agcacgagct caaaaaggcg tttatcagtt attgcagtta aaccaccgag
    14401 ccaaagcagc tgcacagctt ccagctattg agatcgtaga tatgcgtgaa gagtttcaga
    14461 atcatcggac ctctactttt tctgctaatt tgcaggaaaa aatacaaaat cgattagaca
    14521 aaaaagaaca aactgtccta ctgctcaatc gtcgaggata ttcgtctttt gtgatgtgta
    14581 gggactgtgg atttgttctt ccatgtccta attgcgatat ctctcttaca ctgcatatgg
    14641 atacacgttc aatgcgctgc cattattgcg gacatgaaga acctattccc aatcgctgtc
    14701 ctaattgtgg cggcaataaa atccgctact atggaacagg tactcaaaaa gtagaagagg
    14761 aactccgaga actttatccg caagctagga ttttgcgtat ggatgtagat accactcgaa
    14821 gaaaaggggc acacgaacag attttacaaa aattcggtgc aaaagaagca gatatattat
    14881 tagggacaca aatgattgct aaaggtttgg actttccaga agtgacatta gttggggtgt
    14941 tgaatgctga tacctccctg aatttaccgg attttcgttc tagtgagcat acattccaat
    15001 tgcttaccca agtttcagga cgagcagggc gagcggaaaa agcaggcgaa gtcgtgatcc
    15061 aaacattcaa tcctcaacat tacgccatag aattggctaa aaaacaaaat tatgagcagt
    15121 tttatcagca ggaaatgcat gtacggcatc gtggaggcta tcctccttat tactttactg
    15181 tgaaaataac cgccagccat cctgaagaac aagtagcggc aaagaaaatc tttcagattg
    15241 ccaatcagct aaaagaagtg ttaagccctc aaagcttgct attagggcca acgcccagca
    15301 tgattttgag agtgaaaaac cgatattatt atcaactaat catcaaatat aaacatgaac
    15361 cgaatcttcc tcatgttttg gatgaaattt tgaatggaag ccagaaagaa cagcgacaag
    15421 gtctttttgt ggcaattgac aacgaaccat taaactttat ttaagaaagg gccaaattat
    15481 gcgctatcct atattgatcc accctaatga taaattaaaa agaactgctc agccaatcga
    15541 tgtaatcacg gacgagacaa tcgctttatt agacaattta tatgagacaa tgattgcgaa
    15601 tgacggaata gggatcgctg ctcctcaagt aggacaaaac aagagaattg ctgtgatcga
    15661 agtagatgaa ggcgagaaat ttgaactgat taacccagaa atcatcgaag caaaaggtga
    15721 aagccttgac gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg tcaagcgagc
    15781 agatgaagtg accgtgcgtt attatgatcg agacggagaa gagatagaag tgacggcttt
    15841 tggctattta gcccgtgcct tccagcatga aatcgatcac ttagacggta tcttgttcat
    15901 tgagaaaatg atacagcaga tcccggaaga agaacttgaa gaatatatgg aggaacatct
    15961 tgatgactaa gctagtattt atgggcacac ctgctttttc tgtgccgatt cttgaagggc
    16021 tgatagaatc aggttacgag atccaggcag tggtaacaca gcccgaccgt ccagttgggc
    16081 ggaaacgagt aattacgccg acaccggtaa aaaaagcggc tctgaagcac ggtatccgtg
    16141 tgctgcagcc agagaaaatc tccggttcgc cagaaatgga agaaatcatt gaattggtgc
    16201 ctgatgtgat cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta ttgcaagtac
    16261 caaaattagg ggcaatcaat gtccatgctt ctttacttcc taaatatcgg ggaggtgcgc
    16321 ctgttcatta tgccatcatg aatggggaga aagaaactgg tgtaacgatc atggaaatga
    16381 tcaaaaaaat ggatgcaggc ggaatctatg cgcaagaaag cataccaatc actaaacaag
    16441 atgatgtagg gacgatgttt gaaaagctta gccttttagg aagaaagcta ttgctagaaa
    16501 cattgcccaa tattttggat ggacaaaaac cagtacctca agacgagtca gaagcaactt
    16561 tttcgccaaa catcacacgg gaacaagaag caatcgattg gcataaaact gcagaagaaa
    16621 ttgacaacca agtacgcgga atgcgtcctt ggccaattgc attcacgacc tatgagggca
    16681 tacgctggaa attattatcg gttgaagtat tggcagaaac tacagacaaa gcacctggta
    16741 cgatcatccg gaaagacaaa aaaaatctat ggattgcttg cggtaaacag accgtcttag
    16801 ccatcaatga attacagcca gctggtaaag gaaaacaggc cgtacatgat ttccttaacg
    16861 gaagcggtca gcaagttcag attggtcagc aggtgaaata atgaaaaaaa tccctaaaaa
    16921 aatgaaaaaa tcagtacgat atatggcatt actgacatta gagcgagtga acaagggtgg
    16981 cgcctactcg aatcttttgt tgaatgagat gatcaaacaa agcgagctaa atcaaaaaga
    17041 tgtgggttta tttacagaat tgatctatgg tacgattagc catcagcgac tattggaatt
    17101 ttacgttgca ccattgatcg ctaaagcaaa aaaagtggat gactgggtca agatgcttct
    17161 ttatctttct gtataccaat tagaattttt agataaagtc cctgcacatg ctattttaaa
    17221 cgaggcggtc gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat ttgtaaatgg
    17281 tgtcttgagg aattatcagc gtcaaggagc accagatctg caaggcattg ctgatcctat
    17341 cgaaagactt tctgtcaaaa tcagcctgcc tctatggatg acgaaacgac tggtggatca
    17401 aatcggatac gaagaaactg aaaagctcgg tttatcgcta tatcagccta gccatgctag
    17461 tgcccgagta gatacacgcc gactttccag agatcaggcc atcactgttt tgcaagaaga
    17521 agagatcaca gctgaaaaaa gccaagtcag tccatacggt atagtagcga aaaaagggca
    17581 tttagcttct agctcattgt tccacgatgg cgtcatgacg atccaagatg aaagttcgat
    17641 gcttgtagcg ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg cctgtgcagc
    17701 acccggaggt aagacgacgc atattgcttc ttttttagat ccgtcatgcg gtggagaagt
    17761 ggtcgcttta gatgtacacg aacacaaagt caaactcatt gaagataatg cagcacgtct
    17821 aggtgtctct tctgctgtct acgctcaaaa gatggatgct agagaagtca gtaaacagtt
    17881 cgaagcagag cagtttgatc ggattttagt agacgcgcct tgttcaggtc ttgggctgct
    17941 tcggcgaaaa ccagatattc gttataaaaa acagccggat gaattagcga atttaccaaa
    18001 aatccaattg gaaattttag aaagctgcgc accaacttta aagtcttccg gtattctgac
    18061 ttatagcact tgtacgatct taaaagaaga aaaccaagac gttgtttctg ctttcttgga
    18121 aagacaccct gattttgaga ggattgatgt tttgaccaat cctatgctac aatcatcaat
    18181 cgaagagaaa atgttgactt tgtatccgca tcagttttac acagatggat ttttcatttg
    18241 ttgtttgcgc aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat cagatgtagg
    18301 aagacgacgt aacacaaatc aagactatgc cagtgtattt accagccgtt caggtctcaa
    18361 gcttgctgta ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg ccagtcagat
    18421 ggcggtaacg aatctaggcg aagcttggga aaaacaagaa gctgttgacg atgaaaaaat
    18481 cgcccaatgg ttcattcaat cgattcaaga agaaaatatg ctgatttatc agcgtggaca
    18541 agaacagccg gaatataacg gtatgggaac aacaatcgta gcggccgctc tgtcagatga
    18601 ctggttcacg attgccaatg ttggggatag cagagcttat ttgattcgtg aagataaaat
    18661 catgcaattg acggaggatc attctttagt caatgaacta gtcaaatcag gagaaatcag
    18721 cgaagaaatg gcagtgaacc atccaaggaa aaatatttta acacgttctg tcggcatgcc
    18781 tggaacagtc gaagtagatg tttctactca tctttggcaa ctaaaagatc gactgctgtt
    18841 atgttcagat ggcttgacga acatgctgtc tgaagaagaa ataggaacga tcatcaatca
    18901 agaaggccca ttgtctcata aagtaactga gctgattgat cgtgccaatg aagcaggcgg
    18961 agcggataat atcaccgttc ttcttatcga atataaggag gacagtgtat gatcgagttg
    19021 ggcaaaaaat taaatggccg ttatcaaatc actggaaata tcggtagcgg tggtatggca
    19081 aatgtatttt tggcgcacga tttaatcttg gatcgtgatg tagcagttaa agtcttacga
    19141 tttgactttc aaaatgacca gactgcgatt cgacgatttc agagagaagc tttagcagca
    19201 acagaactcg ttcatccaaa cattgttagt gtttacgatg ttggtgaaga agacaacatg
    19261 cagtatctgg tgatggaata cgtcaaagga atggacttga aacgatatat ccaaacacat
    19321 tatcctgttc cttatgaaac agcagtaaat attatgcagc agatcctttc tgctatttct
    19381 ttagctcata accatcaaat catccaccga gatttgaaac cccaaaacgt actgatcgat
    19441 aatgaaggtg ttgtaaaaat cacggatttc gggattgcta tcgctttatc tgaaacatcg
    19501 atcacgcaga cgaatacgat gctaggatct gttcattatc tgtcccctga gcaggccaga
    19561 ggaagtatgg caacgaagca gtcagatatt tatgctttag ggatcatcct ttatgagatg
    19621 ttgacgggta gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt aaaacatttt
    19681 caagatgatc ttccatctat aaaagcactt gatccaaatg tgccacaagc actggaaaac
    19741 gtgattttac gggcaacggc taaagaacct gcagaccgtt ataagtcagc cgaggaaatg
    19801 tctgatgatc tttctacagt attatcacca gctcgtgcaa acgaagaaaa atggcagcct
    19861 catgtgatgg ataatgaaac aaaagttatc acacccctta cggaagatgc accaatgcct
    19921 gattcattca aatctatgcc tctgccaaaa gacaagccag aaaacgaaac cttaccagag
    19981 gaggaagaag ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg gctgatccta
    20041 ttagctattc ttgctgtttt aggaatcgga acagctgtat tctttgcttc tggcgggcga
    20101 ggagaagttg ttgttcctga tgtttctgat ctttctgaag cttctgctag agaaacactc
    20161 gcaaaagcga aattagaagt agcaacaaaa acagaagaaa ttgcagatga caaaatcgaa
    20221 gaaggtaatg tagtgaagac agacccggca gcgggaacga cagtcaagca aaaacgcgaa
    20281 gtcactctgt atattagtag cggcacgaaa aaaatcaaat tagatgatta cagtggtaaa
    20341 agttatgaag atgccgaagc aagtttgaaa gaattagggt tttctgctga cttgattcaa
    20401 aaagaagagg aattcagttc agaggtagaa agcggaatga tcatttcgca atctccatca
    20461 gaagatacag aagtcgatcc taaaacagac acgattactt tcacagtcag caaaggacca
    20521 gaaacagtga ctttgagtag tcatgtagga gaaaactacg aaactgttcg taattggctt
    20581 gttacacaag gattcaatat caataatatt agtgctactt atgactatag cgacactgta
    20641 ccatcaggtt cgatcatcag tcaggatcca gcctcgggtg aagttgtagc agaagaaact
    20701 tacatcaatt ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat ctccggctat
    20761 acaaaaagcg aagcacaaag ttatttagct agtgtcggtg cagaatatat cgggcatgag
    20821 acttccgaat attcaaacac ggtggataaa gataaagtga ttcgtacagt accaggggca
    20881 ggaacgacaa ttaccaaagg aatggttgtg aacgtaattt actctaaagg accagatcca
    20941 gcgtcttcta gttctgaaaa agactcttca gacagttcta gcagctcaag tagctccagt
    21001 agcacaagca actcttcaaa agaagaatct tccagctcca caagttcatc agctagcacc
    21061 tcagaaagtt caaaataagt gctgaataaa atatatcaga agggaatgcg acattgattt
    21121 gttgcattct cttttttgtg tttctatctc ttttgatcaa ttttcaagcc atatactttg
    21181 agaagtttgc atggaataac acctactttc ctcttcgtat ggtaaaataa acaaaaacgg
    21241 aggtggcact tatcaaagga caaatcagaa aagcaatcag cggtttttac tatgtatacg
    21301 caaacggaga gacctatcaa acaagaggaa gaggaaattt ccggaatcgg aaaatcacgc
    21361 cgcttgtcgg agatcaggtg atctttgaga gtgaaaatca aacagacggc tatcttctgg
    21421 aaatcttgcc gagaaaaaat caattagttc gcccaccagt cacaaatgtt gatcttggaa
    21481 tcattgtcac aagtatcgta gagcctgcat tttcttataa tctacttgac cgctttctag
    21541 ttaccttgga atacgagtcg atcgaaccga ttattttttt gacaaagacc gacttgtcag
    21601 acgacccaga aaaaatcgaa gagatcaagc aaacctatac taaaataggc tatccggtga
    21661 ttgtacccaa tcatacgggt gatacgacag aactgatccg ttacttccca gaacggctat
    21721 cggtctttat gggtcagtct ggagcaggaa aatcgacgct actgaatcaa atatcaccgg
    21781 aactgaattt agcaacagga gagatttctg atgcacttgg aagaggacgg catacaacac
    21841 gtcatgtgga actgattcct atctatggcg ggttagtagc agatacacca ggatttagct
    21901 cgattgactt tttgacaatt gaagcaggag aactgccgag acaatttcct gaatttgtag
    21961 aagcttctgt ccattgcaaa tttagagagt gcatgcatgc taaagaacct ggctgcgaag
    22021 taaaacatag agtagaaaca ggtggtatcg cacagacacg ttacgacaat tatttgcaat
    22081 tcttacaaga aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt aaacgaaaat
    22141 aggaggaaac aagaatgaag atagcaccat ccattttgag tgcagatttt gccaatttac
    22201 aaagagacat tgagttagtg gaaaaaggag gcgcagatta catccacgta gatgtaatgg
    22261 atggccgatt tgtcccaaat atcacattag ggccaaatat tgttcaggca atccgtcctg
    22321 ttactaaatt accgttagac gtccatctga tgatcgttga tccagaaaac tacattccag
    22381 catttgcaaa agctggagca gatatcatta ctgtccacgt cgaagcaact cctcatattc
    22441 atcgggcact tcaaatgaca aaaagcttag gagttaaagc tggagtagtt atcaatccag
    22501 gaacaccagt ctccatgatc aaacatgttt tgcctttggc tgatcaagta ctagtcatga
    22561 ctgtcaatcc tgggttcggc ggacaaagct ttattgaaga aacagtagaa aaaattacgg
    22621 aactcgctga tctacgtaga gagaacaatt ggacttatgc aatcgaagta gatggcggaa
    22681 tcgttccaga aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc gttgcaggat
    22741 catatatcta tggagccgaa aatccagtcg gacaaatcga acgattaaaa gaggcattag
    22801 actaaatgaa aattttatta gctgctggag gtaatccgca aaactggccg cagtctctgc
    22861 tgtccaaata cgatgcctat gtcgggatag accgtggtgc gctgtacctg cttcaagcag
    22921 gctacccagt agatctggca gtgggtgatt ttgattcatt gtcgaacgaa gagcagaagg
    22981 aagtatttgc aaaagccaaa gagtataccc gttcccctgc agaaaaagac gatacagata
    23041 cacagttagg tatcttaaaa acatttgaac gttacccgca agcggaagtc actttgattg
    23101 gagctacggg cggaagattg gatcatcttt tagccaatct ctggttaggt atggaacctc
    23161 gcttcaaacc ctttgcccat cagcttatta tcagagattg tcaaaattat ttgtcttatt
    23221 atttgcctgg agagcatata gtaagacaga taccagatat gaagtattta gcttattgct
    23281 gcttgactcc cgtctcgaat cttacattaa aaggcagcaa gtatgtattg gaaaataaag
    23341 aagtagaata tccgacatct tatgcaagta acgaatttct ttcagatacc gcagaatttt
    23401 ctttttcaga tggtatgatt gcagttattc aaagcaaaga ttgatccata aacaaataaa
    23461 aaaatttgtc tttcaggcta aaaatgacag aatctattat ttgctaaaaa aaagcttaag
    23521 aggagcaact taccttttgt ttcacggcat ttagtgataa aattgaacac aagagtctaa
    23581 ttaattcgag gtgtctttgt gaagaagttc aatcctaata aaaatattat tattaccttg
    23641 gttattgtta tcatcgtggt gacgatacta agtgtgaccg ctgcgcagcg agcgaatgaa
    23701 ggaaagccca atttcttcca atccatcgtt aatgacagtg ttgcaatggt tgataaaaca
    23761 atctctgccc cagtacggtg ggtacgaaat ggagtagatt ctgtccataa tcttttttca
    23821 acttatgccg aaaatgagcg gctaaaagaa aaaatcgata gctatgatga cgtttcacag
    23881 caaaacaaga attacgagag agaaatcgaa gcgttgaaaa aagaacttga gttgaatcag
    23941 acgctgacta gttacgaaaa agtaacggca aatgtcatta ctcgttcgcc tgacagttgg
    24001 caggacatgc tgatcgtaga caagggttct aaagacggta tcgaagcaaa tatggcagtc
    24061 atgtcgcaaa aaggattgat tggccgagtg atcgaggtca atacggcttc gtctaaaatc
    24121 gaattactgt catcctctaa tgaaagctcc aatcattttc cagtacgggt atcttcggct
    24181 aatggcgaag cgtttggttt gcttaaaaac tatgatgaaa agctccatgc cttagtggtg
    24241 acccaattaa ctggtgatac ggatatcaaa gaaggggatg ttgtccagac atccggtctt
    24301 ggagggaatt ctccagctaa cttgccgatc ggtacggtta ttaaaacgaa accagatagt
    24361 tatgggctgg atcgggaagt ttatgtgaaa ccttatgcag aaatgtatga cgtgtcagtt
    24421 gtgacgattg tccaacggct agcggaggat tagtccatgt tgaagaaaga aacaatgaag
    24481 tactatctgc caatcgtttt gttctttttg atgttgatag atggtcattt aacaagaatg
    24541 ctaggggagt ggtcgaaagg cacctatatg tcaaatgccc actttctgat attggcatta
    24601 ttatgttgca gtatggcgtt tgaaaaacgt tatttactga ttaccacgat tgttctcggg
    24661 gctatctatg acgcttacta tattggcgtt atcggtatct atgcagtagc tctcccttta
    24721 attgtatggt tgatgtatgt aatgaaagac gttatccatg tcaacatctt tactgaattt
    24781 ttcagtatga tcatctttgt cacgggttat gaattgttta cgatggtggt ccagttgatt
    24841 tttaaattag cagtagtaaa taacacgtat tttattacaa ggtttttagg acctacactg
    24901 ctgttgaaca tgattatatt tgtattattc atttttccct ttaagaaatt attcagtgat
    24961 gaataatttc tttttttatt tttaggtcga aagcgaaaaa gaatcgttgt tatcttaaga
    25021 aatgctagac ttaacgcggc tttttcatga tcattttctg aaacgaacag ttttgtcatt
    25081 tttaaaagtt gttttgaaac atttttgtaa cataactatt atatcgttga cacactgcta
    25141 tggtacaatt tttatatcgt taagaaagac ttaaatatat ttagatattt attaaattaa
    25201 gccagtcgga ggaatgaaga gtgaaaaaga gtttaatatc agcagtaatg gtatgttcaa
    25261 tgaccttgac tgccgtagca tcaccgatcg ctgccgcggc ggacgatttt gattctcaga
    25321 tacaacagca agatcaaaag attgcagact tgaaaaatca acaagcagat gcacaatcac
    25381 aaatcgatgc cttagaaagc caagtttctg aaatcaacac acaagcacaa gacctattag
    25441 caaaacaaga tacattgcgt caagaatctg cacaattagt aaaagacatt gcagatttac
    25501 aagaacgtat tgaaaaacgt gaagatacaa tccaaaaaca agcacgcgaa gcacaagtaa
    25561 gcaacacaag ctcaaactac atcgatgctg tattgaatgc tgattcatta gcagatgcta
    25621 ttggacgcgt tcaagcaatg acaacgatgg taaaagcaaa caacgacttg atggaacaac
    25681 aaaaacaaga taaaaaagct gtagaagaca aaaaagctga aaacgatgct aaactaaaag
    25741 aattagctga aaaccaagct gctttggaat ctcaaaaagg cgatctttta tctaaacaag
    25801 ctgatttgaa cgtactgaaa acttcattag ctgctgaaca agcaactgct gaagacaaga
    25861 aagctgactt gaaccgtcaa aaagcagaag ctgaagcaga gcaagctcgt attcgtgaac
    25921 aacaacgtct agcagaacaa gctcgtcaac aagctgcaca agaaaaagct gaaaaagaag
    25981 cacgcgaaca agcagaggct gaagcacaag ctactcaagc ttcatctact gcacaaagct
    26041 cagcaacaga agaatcatca gctacacaat cttcaatgac tgaagaatca tcatcagcta
    26101 cacaatcttc agcaacagaa gaatcaacaa cacctgaatc ttcaacagaa gaatcaacag
    26161 cgcctgaatc ttcagcaaca gaagaatcaa caacagcgcc tgaatcttca gcaacagaag
    26221 aatcaacaac agtgcctgaa tcttcagcaa cagaagaatc aacaacagtg cctgaatctt
    26281 caacaacaga agaatcaaca acacctgcgc ctacaacacc atcaacagat caaagtgttg
    26341 atacaggaaa cggcacagga agtagtactc cggctccaac gccaacacca acacctgaac
    26401 aaccaaaacc tgtaacacct gctccagctc cttctggaag tgtgaatggt gcagcaatcg
    26461 tagcagaagc ttacaaatat attggtactc catatgtttg gggcggtaaa gatccaagtg
    26521 gctttgactg ctcagggttc acacgctatg tttacatgca agtaactggt cgtgacatcg
    26581 gtggttggac agttcctcaa gaatcagctg gaacaaaaat ttcagtttct caagcaaaag
    26641 ctggagactt gttattctgg gggtcacaag gcggaactta ccacgtagcg attgctttag
    26701 gcggaggaca atatatccat gctcctcaac caggcgaaag cgtaaaagtt ggttcagttc
    26761 aatggtttgc ccctgacttt gctgtcagca tgtaagaatg attaatcaaa aagagttagc
    26821 ccattctgga ctaactcttt ttttattgtg ataaaataga aatcagataa attcttctgt
    26881 caattccatg aacgtttgga tatcttcagt caccatgtcg atacctgctt gccaaaaatc
    26941 tggttgggta agatcgacat tcaaatgttt cttcgcaaga tcttccgtcg tcattgaggc
    27001 agtatcgcgt agtaaatgga tatattcttc ttcaaaattt gctccctttt ggttggcgta
    27061 agcatagatg cccaaactaa agaggtagcc aaatgtgtat gggaagttat aaaacggtac
    27121 gtcatcaata aagaaatgca attttgcagc ccagaaatat ggatggtaag tagctaaagc
    27181 tccttgatag ccttcttttt gcgcctcaac catcatctct gtgatcttat cttctgagac
    27241 taatccttct tgccgtgctt catagaatcg attttcaaaa atgaatcggg aatggatatt
    27301 catgaacata gctaaagcat tttgcagttt tgtatctagc aaattgattt tttcttcttt
    27361 tgtttttgcg acttttaatg tagcatcggc tacgatcagt tctgcgaatg tacttgctgt
    27421 ttctgcgaca ttcatggcat attcgcgatt taaagaaggt aaatcccaca tcgtgctgct
    27481 atggaaagca tgtcctagtt catgagcaag tgtcgcaacc tcattaacag aattactgta
    27541 ggtcataaag atccttgatt cttttgtttc tggtaactca gtacaataac caccaggacg
    27601 tttcccagga cgatcttctg cttcgatcca gctcttttca aaagcagatt gggcaaattt
    27661 agccattttt ggactgaatt tgttaaagtt ttcgataata aatgctgctg cttcgtcaaa
    27721 actaaaggtt ttttctttca aatcgcctaa aataataggt gcatcttgat cttgccattc
    27781 cattttttct ttaccgaaaa gattagcttt tctagtcaag taattaacaa ggggttgttt
    27841 gtttttctgg attgtatccc acatgacaga caatgtttct ttcttcaacc gattgtactc
    27901 taatggtttt tgtagaaaat ctgtgacacc atgaagttca tagtcagaaa gtctaaaacc
    27961 atctaaatga ttcaaagtat ccgtgaagag gggtgccttt tctttccagg cttcttccca
    28021 tgctgcgaaa agtgtttctc tgacttttgg atctggatcg cccatcatct tattgaaggc
    28081 ttgtccagca gataattcga ctacttgtcc atcttgttcg aagggaatcg aaatgctggc
    28141 tacaatcgta tcataatgac tgctccaagc atttagacca tctaaagaaa gcgtatttat
    28201 aatgttttct tcagcttctg ataataattg tgagccatca cgacgaatct cgtttaaacg
    28261 aaaagcaatt gtttcaaacg aagattgaga aagcatctga ttccaatcat tgtcagatat
    28321 ttctgcaaat ttctttgaca atacagtttc ggctgattgc agacgaggca gcattgcgta
    28381 aagcttgcct gataagacct ttgcatctga atcattgaca ttggcagaaa gcaatgcagt
    28441 aatatagctg ctgcattggg taaaaccatt cgtaatcgtt tcttgcaatt gaagaatagc
    28501 gtttaactgc tctgcattgt cagaactagg tgaccatttt gttacgcggt gataatactc
    28561 gttcatttgg tcttccaatt gcttcatccg ttggttcaaa gcatcagaat gactgcctcc
    28621 ggaaaaaata gaatctagat tccaatttaa tgaataactc atgaaaaaac tcccttcata
    28681 ttgtaataca aaattaagta tagcactttt ttatggaaga agtctctatg aaaatgattg
    28741 ttgtttcttc aaaaaaagtc taagataaaa tacagaggtg acttatgaat tcttcaaaaa
    28801 aacaaatgaa aaacggaaat atttatttag taatcgcact gatagtcgtt ataatcttat
    28861 tttacagttc ttctcaaact tacggacagc aatcacaagt ttcgcgatta gaaagctggc
    28921 tgcctaatca gccatttaaa gacacattag cgggtatacg ttttaattat ggtggaagta
    28981 tggtgagtat caaccatctg ggctacgcca aatttttaga gttcttttta aggaagggcg
    29041 cacatttcgg tacctatttt attttgggag gcagcttgtt tttaggtgtt ttccccaagc
    29101 taaaaatttg gtggctgaca gcgatccttg cttggctttc agcaacaggg tacgcaggtt
    29161 tggacgaatt ccatcagatg atgaccggtg gacgaacacc tatgttccaa gatgtcatgt
    29221 tggattcgat cggtgctctg actgctgtga tattatgtgt gctttttgcg gcattcaaga
    29281 agcgtttata gattggctgt ggctttgttt ttttgtgaaa tagtactaga atagaagtgt
    29341 attctcaaaa gaacaaggga ggaaaaatca tgtcaggaca tagtaaatgg cataatatcc
    29401 aaggaagaaa aaatgcccaa gatgcaaaac gagggaaagt ctttcagaaa ctttctcgtg
    29461 aaatttatat ggcagctaaa gcaggcggtc ctgatccatc tatgaatcca gctttgcggc
    29521 ttgttgtgga caaagcaaaa gctgcaaata tgcctaacga caatgtggaa cgggcgatta
    29581 aaaaggctac gagtgccgta gatgaaacga attacgatga aatcacgtat gaaggatatg
    29641 gtccgggtgg agtaggaatc ttagtccatg cattgacaga taaccgtaat cggacagcga
    29701 caaatgtccg agtagctttt actcgaaacg gtggatcact gggagaaacg ggttcagtca
    29761 gttacttgtt tgaccgcaaa ggatatatcg ctattgaacg cgatgggttg actgttgatg
    29821 aagagacgat gtttgaggat gtattagagg cgggggcaga agatctgcaa acttctcctg
    29881 aagtattcga gatttatact gcaccagaag attttgctgc tgtacgagat gagttggaaa
    29941 aagagttcac tttggcgcaa gcagaattga cgatgattcc acaaacgacg attgatttaa
    30001 atgaggaaca aaaagagcaa ttggaacgtc tagtagataa gctagaagac gatgatgatg
    30061 tttcagaagt ctttactgct gctgaaatgt aagtagcaca ttgttagata ataaaaagaa
    30121 agcacctgta ccaatcgtac gggtgctttc tttttatata ttatttttgt tagaagacca
    30181 atacaggcgt tcctttttcg accatcccga ataattcctt cattacactt ggtggggtgt
    30241 tgatgcagcc gtgtgaaccg cgggttttcc ataagtctcc accgtattca ggttgccagt
    30301 ctgaatcgtg gataccaaca cctgtccagt caatcggcat ccagtagttt accggactct
    30361 cgtaaggtgt tccatcgtca tttgttcctt ttaaagttgc atcttcttct ttattccaaa
    30421 catagaagac tccagcaggt gtaggtgtag ttggctttcc ggaaacgata tctgtttcaa
    30481 gggccacttt tccgtctttg taataccaca tatgctggtt ctccagatca acttcgatat
    30541 acgtatcttc gatcaatgga tgatcagcgg ttgtgccacc ttgcacaatg ggtgaacgag
    30601 taaaatcttg gcctgccaga atcgcttttt tcaatgcttc tgtctcactg tctgtttgga
    30661 tcgtccaact gtaagtgcca actgggaccg taacttctcc tctttttgta cttttaaatt
    30721 ttgtgtcatt cgtacttgtg ttatactttg tgcctaaatc tgttacatat tgacgtactt
    30781 gttctgtatc taaatcgact tttccatcat tgtaggtcag ccaactcata atatcacttg
    30841 atgggatctg gaaagtctca ccgtttatac tgtaagtagc ttttacattt gcgatagcat
    30901 tcatggatgc gagttgttct ttcagtgagc tgtcttcaga tgttactttt ggtttttctt
    30961 tgaattcagt caattcaatt gtatctttac cagagtttac cgcacttttt aattgtttga
    31021 ccgcagcatc gacgtcgatc gtatttccat tgacttcagg cttgataacg aaagagtcac
    31081 ctgctttttc gatcgttgca tcagtagtag gtgtccggtc tttgttcaaa tcagtcagct
    31141 tagttgtcaa tgtctgaacg gtagtgtcta gtttttggtg gttttgactg acaggatcta
    31201 tttcttgttt ttctgccgct gagacataag tcattcccca agtccatccg ttttgttcat
    31261 ttaggatcgg agaaagctcc gaagtgaaat catctttgta gccgagctcg gattttggta
    31321 cttcctgcca gacttttcca tcttctttaa tagccagttt tttatcagaa taagcttctt
    31381 ttaatttttc atttgctttt tcgactgtta gattgctgac atttattcca tttatttctg
    31441 tattcggcaa aaaatgcttt gtgtagtatg tactgcgata ggtataaaag ctgatcagca
    31501 ctaagagaac accaagaact gaaagcaaag caacatgttt cgattttttt cgatgcgaag
    31561 atcttgtcat atcgacaact ccttaagtaa aagtttaggg tggaacggtc tcatgatagc
    31621 ataatttaga ggaaaagacg aaggttatct ctactttatg aaaaaattta gaaaccataa
    31681 gggtgataaa ataaaaaaac ggaaaacacc gaatagatgt tttccgtcat gttcaaagat
    31741 tattcagcgt ctagtttagc aaaagcttca tctagaattt cttttaattg cttagcagaa
    31801 gcagccatac ggtcttgctc gctgtcactt aaaggaattt cgattacttt ttggatacct
    31861 tgacggttga taacagcagg tgcaccaatg tagatgtcgt tcaatccata ttcgccattc
    31921 atgaatactg ataaaggaag aacagcattt tcatcatcta agatggcacg agtgatacgt
    31981 gctaatgcta ctgcgatacc atagaaagta gcgccttttt tctcgataat tgtgtaagca
    32041 gcgtcacgta cgccaaagaa taggttaacc atagcttctt catcgatttc tgggttgttt
    32101 tttacccatt catagatttg caagccggca acatttgcat gtgaccaaac tgggaattct
    32161 gaatctccgt gttcacctaa gatgtaagca tggacgttac gtgcatcaac gtcaactaat
    32221 tccgcaagtg cttgacggaa acgagctgag tcaagagagg tacctgaacc gattacgcgt
    32281 tctttaggga aacctgagaa tttccaagtt gagtaagtca aaatatctac tgggttagcg
    32341 gcaactaaaa agataccgtt aaatcctgat tcaacgattt gtgtgacaac atcgcggttg
    32401 atttttaggt ttttgtttac taagtctaaa cgagtttcac ctggtttttg tggtgcgcca
    32461 gctgtaataa caactaagtc agcatcatgt gcgtctgcgt aagtagcaga atagattttt
    32521 ttaggtgaag taaatgccaa agcgtgagac aagtcaattg cgtctccttc agttttagca
    32581 gtattgatat ccacaatacc tacttcttga gcgatatttt gagtaactag agcaaaggca
    32641 tagctagaac ctacggcgcc gtctccaaca agaataactt tttgatgatc ttttttttct
    32701 gcagctgcag tcatttagta tcaatccttc cattgatttg ttttttccca tgcttatgct
    32761 aacatttctc aagccccatg tcacgctatt tggctataaa atataacaga taacaaggtt
    32821 gtattatatg taaaagctta catgtgcgtt atttcactat ttttatatat caattatttc
    32881 attaaacttt gaagaaattt ttaatttctt tatcatttat taacgattct cttttagccg
    32941 aaacgccttg ttttgtgtta tactatgcat tgacaagtgt aaaaaagaaa aaagctcgat
    33001 ggactggacc gctaattagc agtccagcct tttgtgctgc tttttagaat taggtggaaa
    33061 acaaaaatga aaatgatcgt cgggttagga aacccaggta ccaaatacca atatacaaaa
    33121 cacaatatcg gctttatggt cgtggataaa attgcacgcg agcatcaagc tactttcaaa
    33181 aaaaatccgt tcgaagcaga agttgccgaa ttttttcata atggagaaaa gatcctttta
    33241 gtcaaaccac aaacattcat gaatgaatct ggtcgtgcag tcggcccact aatgacttat
    33301 tttgggattt atccagagga attagtcgta atctacgatg atttggatct ggcagtggga
    33361 aaaatccggc tccgtcaaaa gggaagtgcc ggcgggcata acggtatcaa aagtattatt
    33421 tcccatttga atacgaatgt ctttgatcga atcaaggtgg gaatcggcag gccagaaggg
    33481 aaaaaaacag tagtccagca tgtgcttagt cctttcagca aagaaaatca gcctttgata
    33541 gaagaaagca tgtgtcaatc tgttaaagca gtcgaatatc tcatagaagg gcattcgttc
    33601 gtggatgcga tgaatcgatt caattgagga aggaggaaaa ggaatggata ttattcaatt
    33661 agtcggaaaa gatccgcaag taaaagaatg ggtcagcaat ttagaagaaa aaatgcctag
    33721 acaactaatc acaggattag cgggctcagc gaaaacactt ctatttgctc gagcgtttaa
    33781 gaaaacaaat aaaaacatga ttattctgat gccaaatttg tattatgcga atcagttagc
    33841 agaagatctg caacatgtga ttccgcaaga tcagatccat ttatttccag tggatgaagt
    33901 gctttctgct gaaatggcct tttcttctcc agaagcgcga ggcgagagag ttgcaacgct
    33961 taacgcactt caagaaaaaa aagcaggtat ctatttgttg ccagtcgcat ctttgcacaa
    34021 acgactgccg aataagaaaa cttggctaca ggcgcaattg aattggcata ttggtgatga
    34081 gatagacgtg gaaaatcttc ctcgccaatt gactttgatg ggctatgtac gagaagagat
    34141 ggtcgcaaaa ccgggagaat tcagtattcg aggaagcatt atcgatattt acccgttaac
    34201 gacaacctat cctgttcgtg tagaactatt tgatgtagag atagattcta tgcggtattt
    34261 tgaagcagaa acacaacgct caatcgaaaa aacggaagaa atctgggttt ccccaacgag
    34321 tgaacaagtc tacggaccag aggatctaga acatggaata gagcaaatgc aaaaaatgct
    34381 agaaaaacgg cttgctgcga ccacagaaga agcagaccgc gagtttttgc aagattactt
    34441 cggtcagctg acttcaagct ggcaagcagg tgtcccgaca gaagaagcaa aattctactc
    34501 agatcttctt taccaagaat ctgtgacgat cttggattat ttcccttcaa atagtttact
    34561 agtcgttgat gattatcagc gaatcatgga aacaaatcgg gaaatcgaaa gggaagcggc
    34621 agaatggcag actcaaaaaa tcagcgaatt gcgggtgttt tctgagcaga cattttcagc
    34681 agatatacaa aaaatcgtgc agaaggaaaa gttcaccaca acctttttct cactcttcca
    34741 aaaagggatg ggaaatcttc gtttccaagc aatccataat tttcaatatc ggacgatgca
    34801 acagttcttc ggacaaatgc cattgctgaa aacagaaatg gaccgttggc gcaagcaaaa
    34861 acagactgtt gtgatcttta ttccgacaaa ggacagaata cgcaaagcag aacaaatgct
    34921 tcgagatgaa gacatattag tagtagaaac agaaaaaaat gcattgatca gaggacaggt
    34981 acaattagtt gaaggagctt tgcaggctgg ttttgaactt cctcaagaaa aaatagtagc
    35041 tatcacagaa aaagaaattt tccaaaaaac gacgaaaaaa caaacgagac gtcaaacagt
    35101 aacaaacgca gagcgattga aaagctataa tgagttaaaa gcaggagatt acgttgtcca
    35161 tgccaatcat ggtatcggga aatacattgg gatggaaacg ctagaagtag atggcgttca
    35221 ccaagactat atgactattt tgtatcaaaa cgacgacaaa ctgtttattc ctgttactca
    35281 actgaatctg atccaaaaat atgtcgcctc tgaagccaaa gcgcctcgca tcaataagct
    35341 aggcggaagt gaatggacga aaaccaagcg aaaagtctct tcaaaaatcg aggatatcgc
    35401 tgacgatcta atcaaattat atgccgcaag agagtcagaa aaaggatatg cttttggacc
    35461 tgatgacgcc taccaaaaag aattcgaaaa tgccttcccc tactctgaaa ctgatgacca
    35521 gttgagaagt gcagcggaga tcaaacggga tatggaaaaa gaaaaaccaa tggatcgact
    35581 actcgtaggt gatgttggtt atgggaaaac agaagtagcg cttcgagcgg cctttaaagc
    35641 gataaaggaa agcaaacaag tagccttttt agttccaaca acgatcttgg cacagcagca
    35701 ctatgaaacg atgctggaaa gatttgaagg tttccccgta aatatcgggc tattaagtcg
    35761 tttcagaaca agaaagcaac aaaaagaaac catcgaacaa ctgcgtacag gtcaagttga
    35821 tattgttgta ggaacccacc gaattttgtc aaaagatatt gaatttagcg atttgggtct
    35881 actgatcatt gacgaagaac aacgttttgg tgtaaaacat aaggaacgat tgaaacagtt
    35941 acgtgcacaa gtggatgttt tgactctgac tgcaacaccg attccgagaa ctttgcatat
    36001 gtcgatgtta ggcgtgcggg atctttcagt aatcgaaaca ccgcctgcga atcgttatcc
    36061 gatccaaacg tatgttatgg aaaaaaatcc tggggctatt cgcgaagcaa tccatcgaga
    36121 aatgggacga ggtggacaag tattctattt atataatcgg gtagaaacga tcgaacaaaa
    36181 agtagaagaa atccaagaac tcgttccgga agcacggatc ggttatgctc atgggcaaat
    36241 gacagaggcg caattagaaa atacgctttt tgaatttatt gaaggtcaat atgatgttct
    36301 tgtcactaca accatcattg aaacaggagt agatatcccg aacgcaaata cgttgttcgt
    36361 tgagaacgct gattatatgg gattatccac gttataccaa ttgcgagggc gggtaggaag
    36421 aagcagccgt gtcgcatatg cttactttat gtatgagcag cagaagatcc ttaacgaagt
    36481 cagtgaaaaa cgtcttcagg caatcaaaga ttttacagaa ctgggatctg gattcaagat
    36541 tgcgatgcgg gatctttcca ttcgcggtgc aggtaatctc ttaggtgctc agcagcatgg
    36601 atttattgat gcagtcggtt tcgatatgta ttctcaaatg ctaagcgaag ctgtttctcg
    36661 taaacaagga aagaatagtc aagtggagaa gaccactgtt gagatcgacc tcggtgtcga
    36721 tgcgtatcta cctgaaacat acgtggcaga tcaacggcag aaaatcgaga tctataaacg
    36781 aattcgtgaa cttgattcgc aagaaatgct agatgaacta gaagatgatc tgctcgaccg
    36841 ttttggagaa tatccagaag aagtagcaca tctcttgaca attgggcaga tcaaaatgga
    36901 tggcgatcga gcgctcctag aaacgataag gaaacaggag gcgcatatag tctttactct
    36961 aagtaaagtt ggaacaaaac gctatagtgt ggaacagtta ttcgaagcac tgacagcgac
    37021 aaaactaaaa gccagtctag gcgttgaaaa agaacagatg gtgatacgct tgacgatccc
    37081 caaccaaatg gaagaagcag tatggcttca ggaaatccaa aagtttgtca aagcgttgcg
    37141 tgagcaaaaa tatctgtcag cgtaggaagg agcaatgatt tggatcataa agaaatgcgg
    37201 cgcgtcatgc aaggagcatt tgtattgacg attgcttctt tcatagcaaa agtattaagt
    37261 gccctttacc gaattcccct ccaaaactta gtcggcgacg aaggttttta tgtttatcag
    37321 caagtttatc caatttatgg gatagcaatg actttagctt tatccggtct tccgcagttc
    37381 atatcaaagt acgttgcaga aagaaagaat cctttcgagc gaaagcaagc tttgcaaaat
    37441 ctttatccct tagtattttg gacaggtgtc tttttgtggt tgtttgtctt tgcatttagc
    37501 agctggattg cttggatgat gggagacata caattgaaac cgctgatcca agtagtttcc
    37561 tttacctttt tgctgatgcc tggtctatct ttttatcgag ggaatttcca aggaagattc
    37621 ttgatggaac caactgctgt ttctcaagta gttgagcagt tcgttcgtgt ttttgtgatt
    37681 gtctgtagtg cgtttgcttt tagaaagctt ggatggacgg tgtaccaaac agggacggca
    37741 gcaatgagtg gagctgtttt tggcggttta tgcgcatatg ggatccttca ctactatgaa
    37801 caaaagattc atggaggagc gttaagcttc cgacggtttc cattgttcaa acagccgtca
    37861 aagcccttga tccggcgttt tctggtagaa ggcggactgg tttcgattta tagtggattg
    37921 cttatctttt tccagctgat cgactctttc tttgtaaaaa atgctttgga agtttacgga
    37981 ctgtcagaat acggtgcgaa gatagcaaaa ggggtctatg atcgaggtca gcctttagta
    38041 caattaggtt tagtgattgc cactgcatta agtgcgacat tcctgcctgc gttgactcgt
    38101 catttgacca accggatcta tcgtcagttt ttacagacag ccaaaatcta tcttagattg
    38161 acgaccgctt tagcattagc tgcgtctttg ggattagcct tgctgctccc ttatatcaat
    38221 tatgcgttgt ttaaagatta tgcgggaaat gcagcgcttg tattatttgt tttttccatt
    38281 gctttcactg cagtcatcca agcttatcaa agtattgctc aaagcaagaa ctcgtttcgc
    38341 ccttctttaa aaggcgcagg atggggacta ttggtaaaag gattgactac ttcttttctg
    38401 accggtttat taggaacagc aggtgcaagc ctgtctaccc ttttaggatt aggtgtgacg
    38461 ctttggtatt ttgttcgaat tgaaacaaaa gagctaaatg ctttttggaa agaacggcat
    38521 tttggacgga agttgatgtt cagccttctt tgtatgacag tagtactgct gctgtattat
    38581 ggtgtattga atatagtatt tggtcctgta actcatcgtt ccactgcttt ttttgtcagc
    38641 gtgataggag ttgggatcgg ggcttctgtg ttcatttatg cagtgattcg tcttcgtctg
    38701 tttacagtac gagaatggct aatgttgcct tttggtaaga aaatcctgcg attaaataaa
    38761 aaaattaa
//
LOCUS       DAZUKR010000013        35398 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000013 DAZUKR010000000
VERSION     DAZUKR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.013"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACG89C_000605"
     CDS             <1..649
                     /locus_tag="ACG89C_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899183.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACG89C_000606"
     CDS             708..1148
                     /locus_tag="ACG89C_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899184.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACG89C_000607"
     CDS             1160..1828
                     /locus_tag="ACG89C_000607"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4899185.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACG89C_000608"
     CDS             1892..2515
                     /locus_tag="ACG89C_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS4899186.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACG89C_000609"
     CDS             2571..2957
                     /locus_tag="ACG89C_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS4899187.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACG89C_000610"
     CDS             3170..4561
                     /locus_tag="ACG89C_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS4899188.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="ACG89C_000611"
     CDS             5172..5540
                     /locus_tag="ACG89C_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4899189.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="ACG89C_000612"
     CDS             5720..6661
                     /locus_tag="ACG89C_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4899190.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="ACG89C_000613"
     CDS             6730..7461
                     /locus_tag="ACG89C_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4899191.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="ACG89C_000614"
     CDS             7605..9785
                     /locus_tag="ACG89C_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4899192.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="ACG89C_000615"
     CDS             9805..10287
                     /locus_tag="ACG89C_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS4899193.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="ACG89C_000616"
     CDS             10303..11064
                     /locus_tag="ACG89C_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4899194.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /gene="murC"
                     /locus_tag="ACG89C_000617"
     CDS             11195..12529
                     /gene="murC"
                     /locus_tag="ACG89C_000617"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4899195.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="ACG89C_000618"
     CDS             12836..13321
                     /locus_tag="ACG89C_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS4899196.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="ACG89C_000619"
     CDS             13314..13997
                     /locus_tag="ACG89C_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4899197.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="ACG89C_000620"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="ACG89C_000620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4899198.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="ACG89C_000621"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="ACG89C_000621"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS4899199.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /gene="coaE"
                     /locus_tag="ACG89C_000622"
     CDS             17613..18242
                     /gene="coaE"
                     /locus_tag="ACG89C_000622"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4899200.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="ACG89C_000623"
     CDS             18294..18626
                     /locus_tag="ACG89C_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4899201.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="ACG89C_000624"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="ACG89C_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4899202.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="ACG89C_000625"
     CDS             19452..20825
                     /locus_tag="ACG89C_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4899203.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="ACG89C_000626"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="ACG89C_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS4899204.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="ACG89C_000627"
     CDS             21880..22320
                     /locus_tag="ACG89C_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4899205.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="ACG89C_000628"
     CDS             22464..23165
                     /locus_tag="ACG89C_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS4899206.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="ACG89C_000629"
     CDS             23162..24283
                     /locus_tag="ACG89C_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4899207.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="ACG89C_000630"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="ACG89C_000630"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4899208.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="ACG89C_000631"
     CDS             25662..26570
                     /locus_tag="ACG89C_000631"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4899209.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="ACG89C_000632"
     CDS             26605..26877
                     /locus_tag="ACG89C_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS4899210.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="ACG89C_000633"
     CDS             26888..27934
                     /locus_tag="ACG89C_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS4899211.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28185..30794
                     /gene="clpB"
                     /locus_tag="ACG89C_000634"
     CDS             28185..30794
                     /gene="clpB"
                     /locus_tag="ACG89C_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4899212.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30945..31631)
                     /locus_tag="ACG89C_000635"
     CDS             complement(30945..31631)
                     /locus_tag="ACG89C_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899213.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31609..32103)
                     /locus_tag="ACG89C_000636"
     CDS             complement(31609..32103)
                     /locus_tag="ACG89C_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4899214.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32123..33343)
                     /locus_tag="ACG89C_000637"
     CDS             complement(32123..33343)
                     /locus_tag="ACG89C_000637"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4899215.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33490..35325
                     /gene="lepA"
                     /locus_tag="ACG89C_000638"
     CDS             33490..35325
                     /gene="lepA"
                     /locus_tag="ACG89C_000638"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4899216.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11847 a         6250 c         7612 g         9689 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
    28081 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
    28141 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
    28201 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
    28261 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
    28321 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
    28381 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
    28441 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
    28501 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
    28561 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
    28621 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
    28681 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
    28741 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
    28801 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
    28861 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
    28921 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
    28981 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
    29041 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
    29101 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
    29161 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
    29221 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
    29281 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
    29341 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
    29401 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
    29461 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
    29521 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
    29581 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
    29641 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
    29701 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
    29761 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
    29821 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
    29881 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
    29941 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
    30001 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
    30061 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
    30121 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
    30181 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
    30241 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
    30301 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
    30361 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
    30421 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
    30481 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
    30541 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
    30601 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
    30661 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
    30721 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
    30781 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
    30841 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
    30901 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
    30961 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
    31021 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
    31081 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
    31141 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
    31201 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
    31261 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
    31321 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
    31381 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
    31441 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
    31501 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
    31561 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
    31621 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
    31681 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
    31741 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
    31801 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
    31861 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
    31921 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
    31981 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
    32041 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
    32101 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
    32161 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
    32221 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
    32281 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
    32341 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
    32401 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
    32461 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
    32521 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
    32581 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
    32641 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
    32701 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
    32761 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
    32821 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
    32881 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
    32941 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
    33001 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
    33061 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
    33121 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
    33181 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
    33241 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
    33301 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
    33361 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
    33421 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
    33481 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
    33541 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
    33601 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
    33661 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
    33721 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
    33781 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
    33841 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
    33901 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
    33961 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
    34021 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
    34081 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
    34141 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
    34201 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
    34261 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
    34321 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
    34381 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
    34441 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
    34501 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
    34561 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
    34621 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
    34681 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
    34741 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
    34801 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
    34861 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
    34921 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
    34981 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
    35041 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
    35101 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
    35161 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
    35221 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
    35281 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
    35341 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtg
//
LOCUS       DAZUKR010000014        35126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000014 DAZUKR010000000
VERSION     DAZUKR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.014"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1309
                     /locus_tag="ACG89C_000639"
     CDS             164..1309
                     /locus_tag="ACG89C_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4899217.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="ACG89C_000640"
     CDS             1446..1790
                     /locus_tag="ACG89C_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS4899218.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="ACG89C_000641"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="ACG89C_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS4899219.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="ACG89C_000642"
     CDS             complement(3485..4084)
                     /locus_tag="ACG89C_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4899220.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="ACG89C_000643"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="ACG89C_000643"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4899221.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="ACG89C_000644"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="ACG89C_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS4899222.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="ACG89C_000645"
     CDS             7290..8408
                     /locus_tag="ACG89C_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS4899223.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="ACG89C_000646"
     CDS             complement(8468..9760)
                     /locus_tag="ACG89C_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4899224.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="ACG89C_000647"
     CDS             9910..10470
                     /locus_tag="ACG89C_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4899225.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="ACG89C_000648"
     CDS             10599..10976
                     /locus_tag="ACG89C_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS4899226.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="ACG89C_000649"
     CDS             10909..12981
                     /locus_tag="ACG89C_000649"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4899227.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="ACG89C_000650"
     CDS             complement(13192..14139)
                     /locus_tag="ACG89C_000650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4899228.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="ACG89C_000651"
     CDS             14385..14849
                     /locus_tag="ACG89C_000651"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4899229.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="ACG89C_000652"
     CDS             complement(14911..15954)
                     /locus_tag="ACG89C_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS4899230.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="ACG89C_000653"
     CDS             complement(16331..17296)
                     /locus_tag="ACG89C_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS4899231.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="ACG89C_000654"
     CDS             17530..18375
                     /locus_tag="ACG89C_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4899232.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="ACG89C_000655"
     CDS             18566..19753
                     /locus_tag="ACG89C_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4899233.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="ACG89C_000656"
     CDS             19759..20115
                     /locus_tag="ACG89C_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS4899234.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="ACG89C_000657"
     CDS             20117..20455
                     /locus_tag="ACG89C_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS4899235.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20531..20787
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20850..21884
                     /locus_tag="ACG89C_000658"
     CDS             20850..21884
                     /locus_tag="ACG89C_000658"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4899236.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="ACG89C_000659"
     CDS             21877..22563
                     /locus_tag="ACG89C_000659"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4899237.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="ACG89C_000660"
     CDS             22587..23435
                     /locus_tag="ACG89C_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4899238.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="ACG89C_000661"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="ACG89C_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4899239.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="ACG89C_000662"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="ACG89C_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4899240.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="ACG89C_000663"
     CDS             25701..26936
                     /locus_tag="ACG89C_000663"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4899241.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /gene="sufU"
                     /locus_tag="ACG89C_000664"
     CDS             26923..27393
                     /gene="sufU"
                     /locus_tag="ACG89C_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS4899242.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="ACG89C_000665"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="ACG89C_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4899243.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="ACG89C_000666"
     CDS             complement(28884..30026)
                     /locus_tag="ACG89C_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4899244.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="ACG89C_000667"
     CDS             complement(30065..30799)
                     /locus_tag="ACG89C_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4899245.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..31306
                     /locus_tag="ACG89C_000668"
     CDS             31031..31306
                     /locus_tag="ACG89C_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899246.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31404..33401
                     /locus_tag="ACG89C_000669"
     CDS             31404..33401
                     /locus_tag="ACG89C_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899247.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(33477..33812)
                     /locus_tag="ACG89C_000670"
     CDS             complement(33477..33812)
                     /locus_tag="ACG89C_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4899248.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            33939..34154
                     /locus_tag="ACG89C_000671"
     CDS             33939..34154
                     /locus_tag="ACG89C_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4899249.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            34191..34553
                     /locus_tag="ACG89C_000672"
     CDS             34191..34553
                     /locus_tag="ACG89C_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS4899250.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            34599..34925
                     /locus_tag="ACG89C_000673"
     CDS             34599..34925
                     /locus_tag="ACG89C_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899251.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        11576 a         6221 c         7357 g         9972 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    31321 ctaaaacata tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag
    31381 aattttataa ggaggaaaca caaatggaaa catcaatgat taatattcca atttatgtag
    31441 atcaagaaaa attattagag aagccagtta caaaaaaaga aaaaactgat gtaagagata
    31501 ggtgtttaga taactataga gaagttacaa gagatgagtt tgtagagata ctaaattcat
    31561 cacaatcatt tattccaagc aagacaaaga ataaaggcaa tgacaaggag agttttgtgg
    31621 aaaccagagt aattattctg gatgtcgata acactgttaa agatgagaag gggaaggtag
    31681 tagatttgag taaagatgat agtagatact taagcgtaga aaaggtttta tctatcaatt
    31741 tagttcataa ttctgcgttt gcgatacaaa aaagtattcg gtactctgaa aatctagaaa
    31801 aatacaagat tgtctttcta ttaaaagaag caattactga ctatggtaaa atggttgctg
    31861 tttacgaata cttgaaaaag aacatccccg ggtgtgatga caatgtaaat gcttcgaata
    31921 gaatgttttt tggaggatat aagtcagatg cagtgataat aattaatgaa gacaacatgt
    31981 tagatattac tgagcttccg attgatttta ccatgacatg ttctaaaaat tgtcaaagtg
    32041 attcagggag agagtatgaa ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg
    32101 acgacaaaga agaaatgaaa cagtggttta aagactgcct gtttgattcc tccgacatgt
    32161 cttttaatga gatttatgaa agattgttaa ccattgatat gaataaatta cttaagtcaa
    32221 gtaaaaactt aagatgtctg tttcacaacg atcataatcc ctctgctagt atttttacca
    32281 gtaaaactgg gcattccatt ttttattgtc attccagtaa ttgtgatatc agtgaaaatt
    32341 ttattggagt tgtgatgcta attttaaaga aagacagtcg ggtagaaaca tttaattggc
    32401 tattagagaa cctagacttg tatcctacac attttcgaaa attgaaagag gaatcaaaag
    32461 tactttttga cactcttgaa tcaatgaaag acaaactttt caggacaata aggaattatc
    32521 tagaggaaat gcgacgtgtc tatgatgtct tgctttctga tgttaatttt tttgatgata
    32581 gtaaggaaag tgtgacttgt attttgtcgg gggaacaatt agctaaaaaa atgagttctt
    32641 actatggtaa aacttatgac atagataaat gtaataagtt actatcactt atgactttta
    32701 taggattgat aaaaaaatta gatgatgaag atgttccttt gaagatgctc gaatacctaa
    32761 ataggttaaa ggaagagaaa gcgttaggaa actttaatca taagagaagc aatgtctatc
    32821 gattagacgt ttttaatgca acttctgtga ttcaaaaatt gaatgaagat atccttccac
    32881 ttctggaaaa aattcatttt acatatcaat atttttctta tgaatgggtt aagatatgtt
    32941 ttaacgaaaa agaggctaaa agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg
    33001 aaaaacaatt gattttagat gaagcacatg aagttttaca gggcttatcg tggcaaggga
    33061 catatctaat gacagagaaa gaacttatta acactgtttg tgaacgccta cgtaatgttg
    33121 gggataacag cgtgaaaaat gctttaataa aaaacagagg ctactttgtt aaacaaggat
    33181 tcactcttga acgagcttct aaggatatta agaaactata caatgtgaaa gcaagtagta
    33241 gttttttaat atatttagcg aaaggggcag ggatagatga aacaataaaa gaattgatat
    33301 tatcaggagc tgggatagtt cctctgtatc aatttacaag tgtaacaaga gatgatggac
    33361 ggagagtaag gaagagaaaa gacccaattg tattaaacta gacaggttat ccatagctct
    33421 taatcttatt tcatctcaat acacccaccg ttaaagttcg gtgggttatt tatttttcaa
    33481 tctttcaata gctgattgcg ttcattttct tgccttttta atagcttcat atagtcaatt
    33541 gttcgaactg catcttcagt gctaagagaa cgatagagag ccaaaaactc ttcctcataa
    33601 tctacgacaa cattgtctag aaaatggctt acaggggttc ctagggctct agaaagagct
    33661 gttaagctat cgaggttagg ctctgttaca gaattttccc atcttgctac tgcctgttgt
    33721 gtagagaaga ttttttcagc taattctttt tgtgtccacc cgagagcttt tcgcttttct
    33781 ctaatgacct ttgcatattg tttctgtttc attaatacac ccccatctca gtatagtaca
    33841 acaagttgtt gtatttgtaa tggtatttga agttttttat attttatgag ttgaaataca
    33901 tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta agtaatctca
    33961 gccgatacag aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa atgaatgtta
    34021 cacagcaatg tgtaagttcg tggcaaacag ggagaacgat tcctaaaccc tatcaaatga
    34081 agatgctttc tgagatatta agtgtaccaa tgaatgagct gttttctgat gtatttaata
    34141 aagtaaacag ttaactaata agcaattgaa tgattttagg aggagaagac atgtattgta
    34201 gaaaatgtgg caaagaaata acagatgaaa gtaagtattg ccagttttgt ggagcactac
    34261 aagaaacaga tgagacaacc ggaacagaca cagtaaagaa aagtactgag cagacaatta
    34321 atgaaatgaa ggaacaaatc gtacatggac aagagacagg gaagctgtat ttgattattg
    34381 gttggatttc tatggtagta tcgttagcat ttattcctgt tgtatttggt gctgttgctg
    34441 ttattatggg ctacttatat cgagaaaaag atgagaaaat gggtacgatt ttgatgatag
    34501 caggggtagc tggagcaatc tttggtgtac ttttagggat gtcaatgtac tgataagagg
    34561 gataagagca aattattttg gagataggag ataaaaagtt gaataaagta aatatgaaca
    34621 agaagtttgt cattataggt attgttgtct tggctattat aggtattatt ttttcaaaaa
    34681 tgacaggtac atctggatta gagaagtcat caaaaccatt ggtaacacag attttacaag
    34741 aacagtacaa cttaaatcga tcatgtgatg atgtgacgat tactcatgaa aatggagata
    34801 acacataccg ggctaaagct atattagaca atggaagtgc tatcaacata aatattgagt
    34861 actatccaaa gaaagaccgt atatatgtgg agataccata tgctgaggtg ttaatgttaa
    34921 attgatttaa atgtattatg cataccgtcc aaacaggaac tcccaaaggt gtaaattcac
    34981 tagggagttt ttatagtttc ctataaatta aatactaata caggtgacaa aaaaatatta
    35041 caatgattgt cgggaatttt tatgttattc actgttataa caatataaaa gaaatgaaaa
    35101 agaaaaaatt attatggata tgtttg
//
LOCUS       DAZUKR010000015        34636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000015 DAZUKR010000000
VERSION     DAZUKR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.015"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG89C_000674"
     CDS             591..1280
                     /locus_tag="ACG89C_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4899252.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG89C_000675"
     CDS             1267..2475
                     /locus_tag="ACG89C_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4899253.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG89C_000676"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG89C_000676"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4899254.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG89C_000677"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG89C_000677"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4899255.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG89C_000678"
     CDS             complement(6578..7273)
                     /locus_tag="ACG89C_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS4899256.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG89C_000679"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG89C_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4899257.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG89C_000680"
     CDS             complement(8472..8663)
                     /locus_tag="ACG89C_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS4899258.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG89C_000681"
     CDS             complement(8684..9574)
                     /locus_tag="ACG89C_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4899259.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG89C_000682"
     CDS             complement(9561..10328)
                     /locus_tag="ACG89C_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4899260.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG89C_000683"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG89C_000683"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4899261.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG89C_000684"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG89C_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4899262.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG89C_000685"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG89C_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4899263.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG89C_000686"
     CDS             14899..15282
                     /locus_tag="ACG89C_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899264.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG89C_000687"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG89C_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS4899265.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG89C_000688"
     CDS             complement(16226..17044)
                     /locus_tag="ACG89C_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS4899266.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG89C_000689"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG89C_000689"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4899267.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG89C_000690"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG89C_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4899268.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG89C_000691"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG89C_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4899269.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG89C_000692"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG89C_000692"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4899270.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG89C_000693"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG89C_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS4899271.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACG89C_000694"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACG89C_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4899272.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG89C_000695"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG89C_000695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4899273.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG89C_000696"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG89C_000696"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS4899274.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG89C_000697"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG89C_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4899275.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACG89C_000698"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACG89C_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4899276.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG89C_000699"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG89C_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4899277.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACG89C_000700"
     CDS             28352..30325
                     /locus_tag="ACG89C_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS4899278.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACG89C_000701"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACG89C_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4899279.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG89C_000702"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG89C_000702"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4899280.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACG89C_000703"
     CDS             33204..34496
                     /locus_tag="ACG89C_000703"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4899281.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAZUKR010000016        33689 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000016 DAZUKR010000000
VERSION     DAZUKR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.016"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..820
                     /locus_tag="ACG89C_000704"
     CDS             <1..820
                     /locus_tag="ACG89C_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4899282.1"
                     /translation="KKSKEYANIKIIKGYVRSMFDIAEILNYIEFNRTTKIIQSITAP
                     KKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSE
                     TYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQAD
                     QLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRH
                     TYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
     gene            complement(908..1300)
                     /gene="rpsI"
                     /locus_tag="ACG89C_000705"
     CDS             complement(908..1300)
                     /gene="rpsI"
                     /locus_tag="ACG89C_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4899283.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1316..1744)
                     /gene="rplM"
                     /locus_tag="ACG89C_000706"
     CDS             complement(1316..1744)
                     /gene="rplM"
                     /locus_tag="ACG89C_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4899284.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2278..2745)
                     /locus_tag="ACG89C_000707"
     CDS             complement(2278..2745)
                     /locus_tag="ACG89C_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4899285.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2865..4403)
                     /locus_tag="ACG89C_000708"
     CDS             complement(2865..4403)
                     /locus_tag="ACG89C_000708"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4899286.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4483..5169
                     /locus_tag="ACG89C_000709"
     CDS             4483..5169
                     /locus_tag="ACG89C_000709"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4899287.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5370..5897)
                     /gene="msrA"
                     /locus_tag="ACG89C_000710"
     CDS             complement(5370..5897)
                     /gene="msrA"
                     /locus_tag="ACG89C_000710"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4899288.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5981..6658)
                     /gene="pgmB"
                     /locus_tag="ACG89C_000711"
     CDS             complement(5981..6658)
                     /gene="pgmB"
                     /locus_tag="ACG89C_000711"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4899289.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6655..7785)
                     /locus_tag="ACG89C_000712"
     CDS             complement(6655..7785)
                     /locus_tag="ACG89C_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4899290.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7785..9968)
                     /locus_tag="ACG89C_000713"
     CDS             complement(7785..9968)
                     /locus_tag="ACG89C_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS4899291.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9965..10990)
                     /locus_tag="ACG89C_000714"
     CDS             complement(9965..10990)
                     /locus_tag="ACG89C_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4899292.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11035..11862)
                     /locus_tag="ACG89C_000715"
     CDS             complement(11035..11862)
                     /locus_tag="ACG89C_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4899293.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11859..12926)
                     /locus_tag="ACG89C_000716"
     CDS             complement(11859..12926)
                     /locus_tag="ACG89C_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4899294.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12952..13788)
                     /locus_tag="ACG89C_000717"
     CDS             complement(12952..13788)
                     /locus_tag="ACG89C_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899295.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13785..14654)
                     /locus_tag="ACG89C_000718"
     CDS             complement(13785..14654)
                     /locus_tag="ACG89C_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899296.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14724..16007)
                     /locus_tag="ACG89C_000719"
     CDS             complement(14724..16007)
                     /locus_tag="ACG89C_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4899297.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16021..17706)
                     /locus_tag="ACG89C_000720"
     CDS             complement(16021..17706)
                     /locus_tag="ACG89C_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4899298.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17981..19006
                     /locus_tag="ACG89C_000721"
     CDS             17981..19006
                     /locus_tag="ACG89C_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4899299.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19601..23254)
                     /gene="rpoC"
                     /locus_tag="ACG89C_000722"
     CDS             complement(19601..23254)
                     /gene="rpoC"
                     /locus_tag="ACG89C_000722"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4899300.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23333..26950)
                     /gene="rpoB"
                     /locus_tag="ACG89C_000723"
     CDS             complement(23333..26950)
                     /gene="rpoB"
                     /locus_tag="ACG89C_000723"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4899301.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVYYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27678..28646
                     /locus_tag="ACG89C_000724"
     CDS             27678..28646
                     /locus_tag="ACG89C_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4899302.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28664..29569
                     /locus_tag="ACG89C_000725"
     CDS             28664..29569
                     /locus_tag="ACG89C_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899303.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29566..30372
                     /locus_tag="ACG89C_000726"
     CDS             29566..30372
                     /locus_tag="ACG89C_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899304.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30529..31497
                     /locus_tag="ACG89C_000727"
     CDS             30529..31497
                     /locus_tag="ACG89C_000727"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS4899305.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31814..32338)
                     /locus_tag="ACG89C_000728"
     CDS             complement(31814..32338)
                     /locus_tag="ACG89C_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS4899306.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32625..>33689
                     /gene="scm"
                     /locus_tag="ACG89C_000729"
     CDS             32625..>33689
                     /gene="scm"
                     /locus_tag="ACG89C_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4899307.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         9548 a         7196 c         6320 g        10625 t
ORIGIN      
        1 caaaaaatcg aaagaatatg caaatataaa aatcataaaa ggatatgttc gttccatgtt
       61 cgatattgca gaaatactaa attatatcga atttaatcga actactaaaa ttattcaaag
      121 tatcactgca cctaaaaaaa atgctttaga agaaaaaaga atacaagaag gtaagcaagc
      181 actatcatca aaagaactta ctaattggat agaagcagta aatgatgatt ttaataatca
      241 tttacttact tttcatgatt acacactttt tatgattacc ctgtatcttg gtgatagaaa
      301 aagtgagact tacgctttgc aatggaaata tattgatttt gaaaaacaaa cggtccgtct
      361 taaacatact ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa aagataccgt
      421 cattcaagta ccagaagttg taatgacatt gctaagtgaa tggaaatcgg tacaagctga
      481 tcaattgttg aaattaaaaa ttaagcaaac gttagatcag tatctcttta cttatactaa
      541 accatctgga gaagtaaatt gtcctgttca tgccgattac ctaaactatc gaatcaattc
      601 aattaaaaga aggcatcctg acttagttca tttatctcca cacaaactcc gtcatactta
      661 tgcaactata gctagacaag gtggagcaga tatgaatcaa atttcaaatg ctttaacaca
      721 ctcagatatt tctacaacaa agatatatgt gaacacacca gatatagttg ataaggctgt
      781 ttttgaagct tttcagagag gattgaataa gtgtgattga tatgtgttct taaatgtgtt
      841 cttaaaaaca aaagaagcac tttccaaata attgaaaagt gccagaaacg ttgatatata
      901 aaggatatta acgttttgag aattgtgaag ctttacgtgc tttcttaaga cctggttttt
      961 tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga
     1021 agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc
     1081 cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa
     1141 cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat
     1201 cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt
     1261 ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga
     1321 ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac
     1381 cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt
     1441 ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg
     1501 atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac
     1561 ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag
     1621 gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc
     1681 ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg
     1741 ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac
     1801 ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat
     1861 ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt
     1921 tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga
     1981 caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg
     2041 tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac
     2101 tcggagctga acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc
     2161 ggtattagct caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata
     2221 ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta
     2281 cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt
     2341 ggctgttttg aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac
     2401 ttcgattcct ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa
     2461 acgttctgga atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc
     2521 aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc
     2581 atctaactga gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa
     2641 acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc
     2701 tgcaactaat tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta
     2761 acattttctt acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt
     2821 acctgagaac gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca
     2881 gctaattctt ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc
     2941 gaaacaaata atggatagat tttctgatat tttttatgat cagtttcttg tactccaact
     3001 tgctctttca attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt
     3061 ctccagccta aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc
     3121 gggtaaccca gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct
     3181 gtagcacgga tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg
     3241 ttgagacaga tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt
     3301 aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa
     3361 tagggatgaa aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt
     3421 gcatcatatg gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta
     3481 tgccctgccc aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt
     3541 ttctgatcca gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta
     3601 ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca
     3661 cctaacccta gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt
     3721 ggcaaaccta gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga
     3781 aagacttgcg gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat
     3841 tggaattcat ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt
     3901 tcgccaaaca atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg
     3961 aataattcag gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga
     4021 atcggcgtcc cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct
     4081 gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct
     4141 tcatccaaca atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg
     4201 tctgaaggca actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat
     4261 tcctgatctt gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg
     4321 gatgctgctt cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata
     4381 tctaatccga ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta
     4441 gtttaagctt gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc
     4501 attggttgct gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac
     4561 tctattattt atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa
     4621 ttgattccga ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg
     4681 ttgcccttct actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt
     4741 actttttctt ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt
     4801 tcgttgatgg atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca
     4861 attttatgga ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg
     4921 tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact
     4981 ctgttgatgc tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt
     5041 cttgctagtc tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag
     5101 ttggaaaagc tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt
     5161 aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc
     5221 ttaccaaatc cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt
     5281 gttcaatcag ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac
     5341 ggtgttccat aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg
     5401 aaacctgcac gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa
     5461 taatcctgat ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg
     5521 attggcttat cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt
     5581 cgttcttctt catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac
     5641 tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa
     5701 gaaataactt ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt
     5761 gttacttgtt cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa
     5821 atgatccctg gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa
     5881 actgcttttt ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag
     5941 gaggataacg ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt
     6001 ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt
     6061 cagcagaagg aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag
     6121 ctgcttgaca accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca
     6181 attgctgtgc tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc
     6241 cgtcaataag gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat
     6301 ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag
     6361 ctattgcacc aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat
     6421 tgtttttttg ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc
     6481 caaatgacag caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt
     6541 ctttctcagt gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat
     6601 aacgagctgt gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg
     6661 attacctcct tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg
     6721 aagattatca gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg
     6781 aaaaacggcc gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca
     6841 aatagctatc acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc
     6901 ttttagactt tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac
     6961 ccgtttatcg aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga
     7021 agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat
     7081 tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc
     7141 attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa
     7201 aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt
     7261 cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt
     7321 atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt
     7381 actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat
     7441 cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc
     7501 tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg
     7561 gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa
     7621 actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata
     7681 ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc
     7741 tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt
     7801 tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt
     7861 tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga
     7921 tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata
     7981 gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt
     8041 gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca
     8101 atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc
     8161 tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa
     8221 tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg
     8281 acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt
     8341 aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg
     8401 agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta
     8461 tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct
     8521 aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg
     8581 tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg
     8641 ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat
     8701 tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc actgatatga
     8761 tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca
     8821 aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga
     8881 tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc
     8941 aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta
     9001 tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca
     9061 cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac
     9121 tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa
     9181 taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata
     9241 cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc
     9301 gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt
     9361 gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct
     9421 gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc
     9481 aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg
     9541 cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga
     9601 tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca
     9661 tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc
     9721 gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga
     9781 ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt
     9841 ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt
     9901 ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct
     9961 actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat
    10021 tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc
    10081 gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta
    10141 aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg
    10201 cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag
    10261 tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta
    10321 ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc gtaccaattt
    10381 tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg
    10441 tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga
    10501 taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa
    10561 tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct
    10621 ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt
    10681 gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag
    10741 ccaatacatg ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa
    10801 tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa
    10861 agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc
    10921 ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta
    10981 ctttcttcat ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc
    11041 tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga
    11101 ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc
    11161 ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc
    11221 tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt
    11281 caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg
    11341 cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat
    11401 atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac
    11461 tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg
    11521 tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc
    11581 tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag
    11641 acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac
    11701 tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt
    11761 accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat
    11821 gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact
    11881 tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct
    11941 gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc
    12001 atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg
    12061 tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga
    12121 ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca
    12181 tagccgatcc ctattaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact
    12241 gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata
    12301 ggatctagcg tgtaatcggc accgtgtttt tctgctaatt ctctacgctg cgctatggga
    12361 tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg
    12421 atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata
    12481 ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga
    12541 tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt
    12601 ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg
    12661 atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt
    12721 ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga
    12781 cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg
    12841 atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt
    12901 ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt
    12961 tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat
    13021 gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt
    13081 actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat
    13141 cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat
    13201 tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc
    13261 gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa
    13321 tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata
    13381 caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat
    13441 ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa
    13501 agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac
    13561 taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata
    13621 atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga
    13681 atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa
    13741 caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc
    13801 ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct
    13861 gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata
    13921 ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt
    13981 aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc
    14041 tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc
    14101 aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg
    14161 accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc
    14221 aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa
    14281 gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt
    14341 ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg
    14401 cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta
    14461 tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc
    14521 agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca
    14581 gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc
    14641 cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg
    14701 aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga
    14761 tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt
    14821 gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca
    14881 tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct
    14941 ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc
    15001 aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc
    15061 tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca
    15121 gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa
    15181 acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca
    15241 tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa
    15301 aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa
    15361 acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca
    15421 gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt
    15481 tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg
    15541 tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct
    15601 tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata
    15661 acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc
    15721 tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta
    15781 ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca
    15841 aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg
    15901 aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga
    15961 agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc
    16021 ttattttatt attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg
    16081 actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata
    16141 acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg
    16201 aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa
    16261 ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct
    16321 taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat
    16381 ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact
    16441 ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa
    16501 gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc
    16561 gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc
    16621 ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac
    16681 cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga
    16741 tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg aacgaatcgc
    16801 ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt
    16861 cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc
    16921 tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag
    16981 gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa
    17041 tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg
    17101 atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt
    17161 cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct
    17221 tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa
    17281 atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa
    17341 ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc
    17401 ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg
    17461 tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt
    17521 tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc
    17581 ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat
    17641 catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt
    17701 atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga
    17761 aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata
    17821 agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat
    17881 gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga
    17941 attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga
    18001 cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc
    18061 aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga
    18121 accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc
    18181 tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat
    18241 ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga
    18301 agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat
    18361 tttaagctct acgatggatg aaacacttct ttttgaattg gatcaatata atattcctat
    18421 tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa
    18481 tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg
    18541 ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca
    18601 atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg
    18661 cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc
    18721 gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg
    18781 aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct
    18841 attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac
    18901 agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc
    18961 aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa
    19021 agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc
    19081 gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg
    19141 tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt
    19201 aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca
    19261 atccatgtta tttggggata cttatctcta aaacataaat agaataacag acatgtctac
    19321 tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg
    19381 ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga
    19441 ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt
    19501 aagatccttt cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag
    19561 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
    19621 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
    19681 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
    19741 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
    19801 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
    19861 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
    19921 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
    19981 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
    20041 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
    20101 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
    20161 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
    20221 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
    20281 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
    20341 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
    20401 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
    20461 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
    20521 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
    20581 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
    20641 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
    20701 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
    20761 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
    20821 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
    20881 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
    20941 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
    21001 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
    21061 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
    21121 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
    21181 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
    21241 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
    21301 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
    21361 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
    21421 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
    21481 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
    21541 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
    21601 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
    21661 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
    21721 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
    21781 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
    21841 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
    21901 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
    21961 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
    22021 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
    22081 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
    22141 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
    22201 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
    22261 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
    22321 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
    22381 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
    22441 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
    22501 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
    22561 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
    22621 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
    22681 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
    22741 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
    22801 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
    22861 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
    22921 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
    22981 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
    23041 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
    23101 aaatccgctc acagaacaac ccttcacgtt caggttttaa ggtacgatag ttaatcgtct
    23161 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
    23221 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
    23281 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
    23341 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
    23401 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
    23461 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
    23521 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
    23581 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
    23641 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
    23701 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
    23761 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
    23821 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
    23881 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
    23941 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
    24001 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
    24061 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
    24121 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
    24181 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
    24241 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
    24301 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
    24361 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
    24421 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
    24481 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
    24541 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
    24601 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
    24661 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
    24721 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
    24781 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
    24841 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
    24901 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
    24961 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
    25021 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
    25081 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
    25141 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
    25201 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
    25261 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
    25321 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
    25381 gagtttcgat cggacacata cggccatagt gggagtagta aacgtcacga acttcataac
    25441 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
    25501 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
    25561 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
    25621 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
    25681 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
    25741 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
    25801 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
    25861 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
    25921 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
    25981 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
    26041 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
    26101 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
    26161 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
    26221 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
    26281 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
    26341 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
    26401 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
    26461 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
    26521 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
    26581 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
    26641 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
    26701 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
    26761 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
    26821 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
    26881 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
    26941 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
    27001 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
    27061 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
    27121 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
    27181 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
    27241 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
    27301 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
    27361 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
    27421 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
    27481 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
    27541 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
    27601 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
    27661 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
    27721 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
    27781 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
    27841 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
    27901 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
    27961 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
    28021 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
    28081 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
    28141 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
    28201 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
    28261 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
    28321 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
    28381 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
    28441 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
    28501 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
    28561 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
    28621 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
    28681 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
    28741 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
    28801 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
    28861 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
    28921 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
    28981 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
    29041 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
    29101 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
    29161 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
    29221 cggttatatg atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa
    29281 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
    29341 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
    29401 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
    29461 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
    29521 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    29581 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    29641 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    29701 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    29761 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    29821 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    29881 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    29941 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    30001 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    30061 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    30121 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    30181 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    30241 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    30301 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    30361 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    30421 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    30481 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    30541 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    30601 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    30661 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    30721 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    30781 ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt
    30841 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    30901 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    30961 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    31021 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    31081 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    31141 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    31201 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    31261 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    31321 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    31381 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    31441 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    31501 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    31561 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    31621 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    31681 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    31741 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    31801 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    31861 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    31921 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    31981 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    32041 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    32101 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    32161 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    32221 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    32281 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    32341 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    32401 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    32461 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    32521 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    32581 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    32641 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    32701 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    32761 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    32821 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    32881 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    32941 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    33001 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    33061 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    33121 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    33181 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    33241 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    33301 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    33361 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    33421 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    33481 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    33541 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    33601 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    33661 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAZUKR010000017        32207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000017 DAZUKR010000000
VERSION     DAZUKR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.017"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..525)
                     /gene="yajC"
                     /locus_tag="ACG89C_000730"
     CDS             complement(145..525)
                     /gene="yajC"
                     /locus_tag="ACG89C_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4899308.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(622..1767)
                     /gene="tgt"
                     /locus_tag="ACG89C_000731"
     CDS             complement(622..1767)
                     /gene="tgt"
                     /locus_tag="ACG89C_000731"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4899309.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1985..2683
                     /locus_tag="ACG89C_000732"
     CDS             1985..2683
                     /locus_tag="ACG89C_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4899310.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2851..5490)
                     /locus_tag="ACG89C_000733"
     CDS             complement(2851..5490)
                     /locus_tag="ACG89C_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4899311.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6109..7140)
                     /gene="queA"
                     /locus_tag="ACG89C_000734"
     CDS             complement(6109..7140)
                     /gene="queA"
                     /locus_tag="ACG89C_000734"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4899312.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7333..8508
                     /locus_tag="ACG89C_000735"
     CDS             7333..8508
                     /locus_tag="ACG89C_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4899313.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8512..9153
                     /locus_tag="ACG89C_000736"
     CDS             8512..9153
                     /locus_tag="ACG89C_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899314.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9150..10067
                     /locus_tag="ACG89C_000737"
     CDS             9150..10067
                     /locus_tag="ACG89C_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4899315.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10064..10735
                     /locus_tag="ACG89C_000738"
     CDS             10064..10735
                     /locus_tag="ACG89C_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899316.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11276..11764)
                     /locus_tag="ACG89C_000739"
     CDS             complement(11276..11764)
                     /locus_tag="ACG89C_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS4899317.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11965..12231)
                     /locus_tag="ACG89C_000740"
     CDS             complement(11965..12231)
                     /locus_tag="ACG89C_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4899318.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12231..12494)
                     /locus_tag="ACG89C_000741"
     CDS             complement(12231..12494)
                     /locus_tag="ACG89C_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4899319.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13015..13155
                     /locus_tag="ACG89C_000742"
                     /pseudo
     CDS             13015..13155
                     /locus_tag="ACG89C_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13297..15120)
                     /locus_tag="ACG89C_000743"
     CDS             complement(13297..15120)
                     /locus_tag="ACG89C_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4899320.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15718..18423)
                     /locus_tag="ACG89C_000744"
     CDS             complement(15718..18423)
                     /locus_tag="ACG89C_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS4899321.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18729..19676)
                     /locus_tag="ACG89C_000745"
     CDS             complement(18729..19676)
                     /locus_tag="ACG89C_000745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS4899322.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19866..20135
                     /gene="rpsN"
                     /locus_tag="ACG89C_000746"
     CDS             19866..20135
                     /gene="rpsN"
                     /locus_tag="ACG89C_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4899323.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20311..20673)
                     /locus_tag="ACG89C_000747"
     CDS             complement(20311..20673)
                     /locus_tag="ACG89C_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4899324.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20684..21805)
                     /gene="alr"
                     /locus_tag="ACG89C_000748"
     CDS             complement(20684..21805)
                     /gene="alr"
                     /locus_tag="ACG89C_000748"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4899325.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21821..22171)
                     /gene="acpS"
                     /locus_tag="ACG89C_000749"
     CDS             complement(21821..22171)
                     /gene="acpS"
                     /locus_tag="ACG89C_000749"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4899326.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22301..23812)
                     /locus_tag="ACG89C_000750"
     CDS             complement(22301..23812)
                     /locus_tag="ACG89C_000750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4899327.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24134..25486)
                     /gene="murF"
                     /locus_tag="ACG89C_000751"
     CDS             complement(24134..25486)
                     /gene="murF"
                     /locus_tag="ACG89C_000751"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4899328.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25639..26715)
                     /locus_tag="ACG89C_000752"
     CDS             complement(25639..26715)
                     /locus_tag="ACG89C_000752"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4899329.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26911..28656
                     /locus_tag="ACG89C_000753"
     CDS             26911..28656
                     /locus_tag="ACG89C_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS4899330.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28936..29373)
                     /locus_tag="ACG89C_000754"
     CDS             complement(28936..29373)
                     /locus_tag="ACG89C_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4899331.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29357..30730)
                     /locus_tag="ACG89C_000755"
     CDS             complement(29357..30730)
                     /locus_tag="ACG89C_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899332.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31052..31540)
                     /locus_tag="ACG89C_000756"
     CDS             complement(31052..31540)
                     /locus_tag="ACG89C_000756"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4899333.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31562..32164)
                     /locus_tag="ACG89C_000757"
     CDS             complement(31562..32164)
                     /locus_tag="ACG89C_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4899334.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9208 a         6883 c         5943 g        10173 t
ORIGIN      
        1 aagttattta ttattattta tatgacacac ccgattattc aatattgagc aatatatccc
       61 atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt ctgccctacc
      121 tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct cttccagcgt
      181 ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg tttcaacagt
      241 agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg ctgcacgatc
      301 gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat tgtcgatttc
      361 tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt tcatgctatc
      421 taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa aaaaccacat
      481 tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt tcacctcact
      541 aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta gttttttact
      601 taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga acccgtattc
      661 ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt gacgtacttg
      721 tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt
      781 ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac atgtataaca
      841 atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt tcactactag
      901 acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca agacacagtc
      961 aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac cgacacccat
     1021 caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata cacgattcat
     1081 ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga aatccatccc
     1141 gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt ggatgattcc
     1201 aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg cccatcgaga
     1261 cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg gacattcgtc
     1321 aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt tttctggtga
     1381 taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt cttcgatatt
     1441 ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc
     1501 ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac cagggcgtag
     1561 ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca actcttctgg
     1621 ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg tggggaaagt
     1681 tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt tcttgattaa
     1741 acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa aatatcttga
     1801 caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg cttccttcta
     1861 ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa agaaaaacat
     1921 tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa aggagggaaa
     1981 aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg
     2041 gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta ctattcttct
     2101 tattatgatt cctattatct tgtttgccac caccatatat gatggatcag cgagcgtctc
     2161 agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta tttccagcat
     2221 cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt tacgtggaca
     2281 aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg
     2341 cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg gtttgctatt
     2401 agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta tttactacga
     2461 tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg caagtcgaaa
     2521 attgatggat ggttataaag gacaactttt ctggttagat ctttcattta tcggctggca
     2581 tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat acatttctgc
     2641 aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag tgtgacttgt
     2701 gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa acattcagcc
     2761 cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt atagttccag
     2821 taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt ccggtaatcg
     2881 atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc ctaccaagaa
     2941 ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc
     3001 aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt tattggtcgc
     3061 taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca gaatcgatga
     3121 ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt ctacccatgg
     3181 gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga tggtgaagac
     3241 ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt catttttcgg
     3301 aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag caaatcctgg
     3361 aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg gtgctcccca
     3421 tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc ttagcaaaaa
     3481 gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag cgtctacgat
     3541 cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca catctgttcc
     3601 tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat cattcacacc
     3661 atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga tccggatctt
     3721 gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat ctaattcttc
     3781 gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat tcaagatacc
     3841 taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca tgactgtttt
     3901 gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg gtggatcgat
     3961 cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag aaatatcttg
     4021 cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc gtttaccaaa
     4081 gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac
     4141 atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa ctgttgtcat
     4201 catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt cttctaattc
     4261 tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg ttggattacc
     4321 tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata gtgcagccat
     4381 tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta cttcatcttc
     4441 tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg ttttgtctga
     4501 gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga tgatcgcatg
     4561 tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt
     4621 ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc
     4681 tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga tcatactgat
     4741 tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag tattttcgtt
     4801 caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga cgatcgcttt
     4861 cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt cacctaatga
     4921 ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt ctccagtcaa
     4981 cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc
     5041 atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact caacagtggt
     5101 cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca aggattccag
     5161 tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca caacgatagc
     5221 gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg tataaaagga
     5281 aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt
     5341 tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct tttttcgccg
     5401 ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta ccttctttac
     5461 ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc ttcgtacaaa
     5521 aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa agggcttatc
     5581 gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg ctaccaaagg
     5641 atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag aatttctcga
     5701 tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga catgcgtgta
     5761 gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag tatccgaaac
     5821 aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt gaataggaac
     5881 aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta acatgaagaa
     5941 attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt ttatttgaat
     6001 ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt tgtcataaaa
     6061 tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt atttcacgaa
     6121 catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct ggtaagcaga
     6181 taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg attttggcaa
     6241 atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg taatgaaaat
     6301 atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa tcgtttctaa
     6361 tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac gtacttggtt
     6421 caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc
     6481 aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg tcagatagac
     6541 taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat gcaatccagc
     6601 tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc gatctgggtc
     6661 ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt caagaatctc
     6721 tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca attcttcttt
     6781 cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt tggcacgttt
     6841 cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa gaaggacttc
     6901 taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa ctcgtgtatt
     6961 attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga aatgtttatc
     7021 ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa
     7081 tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg ttgttaacat
     7141 atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg ttctgttggc
     7201 aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac taccctattt
     7261 gtattttttc attgttttag gcatactagt aatagatagt tcttcattga aaatagaaaa
     7321 agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc
     7381 gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt tatcggtacg
     7441 agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga tccaacacaa
     7501 gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga attgcgacgt
     7561 cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat ccgtgaaaat
     7621 atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa aacagctgaa
     7681 aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc acatgaatta
     7741 tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc
     7801 atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc cttgcaagac
     7861 cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac ccatgacatg
     7921 gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg tgtgatccaa
     7981 tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga agagttgatt
     8041 ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga agttatgcta
     8101 aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa actgatgagg
     8161 gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa aggatttatc
     8221 gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga cattttgaat
     8281 aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca acgcatttta
     8341 aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg
     8401 actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga tcccgcaact
     8461 actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc tatgcagcag
     8521 ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct ttatatttca
     8581 ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt actgacacgt
     8641 tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac aatcccttct
     8701 cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc tgcgattatc
     8761 gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg gatgaaaaat
     8821 gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt
     8881 ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg tttagctgcc
     8941 gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg tttaggcgat
     9001 ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg tacgatccct
     9061 gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca ccggctgaca
     9121 ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg accatggttc
     9181 ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc aatacagatg
     9241 aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta gcaagtctag
     9301 ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac aatctggcta
     9361 ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca gcggctcgct
     9421 acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat ccgaaaaaag
     9481 cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct
     9541 atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa aaataccatc
     9601 tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt gtagatactt
     9661 cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat ggcttctcct
     9721 tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa
     9781 tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat ctagtcatgt
     9841 tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta gacaacaaga
     9901 tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat accatcagta
     9961 cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa ccatcagtag
    10021 tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc aattggataa
    10081 aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct atatctttgc
    10141 acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag cagttgtcgg
    10201 gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg tgattcgttt
    10261 ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa tcattggatt
    10321 aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct taccgatcat
    10381 caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg tcggcaaagg
    10441 catggggatg actgctagac aacgactatt catggtagag ttaccactct ctgtttctgt
    10501 tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg cgatcggtgc
    10561 attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg caacagaggg
    10621 cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca tcacggactg
    10681 gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac gctgatgtta
    10741 ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt
    10801 catttttaag gcaataatcc actctgattc ttccctcctt atgggtaaag agttgctttt
    10861 cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt tcatatctgc
    10921 atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca ttttcccatc
    10981 actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt tctttgaatt
    11041 gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc atcagtcctc
    11101 gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc ggtaacttcg
    11161 atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa catatctagc
    11221 cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac gattcttatg
    11281 gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga agagcacccc
    11341 cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa aagatatcga
    11401 tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc acgactttaa
    11461 cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta
    11521 aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac catgccaacg
    11581 aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt ttcaacatac
    11641 ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt tggcgataat
    11701 cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg attttccttg
    11761 tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct aaactcaaac
    11821 aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca ataaatccaa
    11881 gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt tgttaactat
    11941 ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat aaatttctac
    12001 aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca gcctgcgtga
    12061 gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca tatatggatc
    12121 ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt
    12181 tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca tcaaagagct
    12241 tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact
    12301 ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc
    12361 agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat ttcagtatga
    12421 ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct aggattcatc
    12481 gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta cttgattaag
    12541 tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct tttttacata
    12601 gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta ctcctttacg
    12661 actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag caaaggtcga
    12721 atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt
    12781 gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta acggttattc
    12841 aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct ctttcgtctc
    12901 ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt tgtgaatcac
    12961 caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga ggctgagatt
    13021 cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata
    13081 ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat caaaatcaaa
    13141 atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc atctttaatg
    13201 gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag cttcaattaa
    13261 gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat ggtagctata
    13321 ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca aacttgtttg
    13381 gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca acacagtagt
    13441 tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga taattggatt
    13501 ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga aatgcttttt
    13561 aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa catgcatcgc
    13621 aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta cccgtgtcac
    13681 attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg catcattttc
    13741 taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa aaatgaacat
    13801 aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga caaacaaaat
    13861 cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca
    13921 tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag caaatggaat
    13981 gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga aaataaacag
    14041 caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta gtcgatcccc
    14101 acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg caagaactgg
    14161 aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag caaattgaag
    14221 gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca aaacagtgac
    14281 ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca cgaagaagaa
    14341 acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc ttggcttctt
    14401 aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag aaccagaaga
    14461 aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg gaagtgcagt
    14521 agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga tcagtaacga
    14581 gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt ccctcagccc
    14641 acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga cttgatgacc
    14701 gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag atactgctac
    14761 tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat tctttccaag
    14821 attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga tgatttgacg
    14881 atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg gtagcgaata
    14941 ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg taccatatgc
    15001 aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg ttaatttgtg
    15061 ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta agtaatccaa
    15121 tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa tttatagtaa
    15181 acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag aaaataattg
    15241 gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca aaaatctaac
    15301 atttttttat gggctttttc tttgaattct ataataaaag gatagaactt gacttagaaa
    15361 atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag gctgctttct
    15421 acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg ataaacggtg
    15481 tttaaaagct tgcatctgcg gtaataagcc caaccaaatt aaaaacataa gaagctgttt
    15541 ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc ttggtacacg
    15601 gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat gaggaactat
    15661 ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc tcttttttta
    15721 atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt cttcgatgaa
    15781 cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca tgatccagat
    15841 tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca agtttaccgc
    15901 atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga cttggatcgg
    15961 atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga tccctttacc
    16021 gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta aggcgatcaa
    16081 gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta agaaaatatt
    16141 ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca gcatacgagc
    16201 aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat agtattctgt
    16261 cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag tataaaacgg
    16321 ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta gctcaacagg
    16381 ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga tgacttcgcc
    16441 tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga taccagcttt
    16501 gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg tttcgacttc
    16561 tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta gtgctggcca
    16621 atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg gtaattcatt
    16681 gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat ttccattcag
    16741 cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt gatatttttc
    16801 taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg aatcaaagtc
    16861 gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga tgacttggtc
    16921 tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa tgatgccatt
    16981 gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct tcgtgtcttc
    17041 attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta gcaagggttt
    17101 taattgattt agctgatcaa taattggctg taatttcgta tttccagcac ttgtttgcaa
    17161 tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata ttccgtcaat
    17221 aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct taccatcttc
    17281 aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt gtccaattga
    17341 cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca gctgagaggc
    17401 acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt tcacatctgg
    17461 caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat cgattccttg
    17521 gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt gcttaccggc
    17581 attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt gatctttcaa
    17641 ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta ctttagggat
    17701 gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta gttgactttg
    17761 taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat ctgttgataa
    17821 aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt aacctatctc
    17881 attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg tgatttgcga
    17941 ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga ttttttcatt
    18001 taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca gcagatcttc
    18061 tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga ctccgtcttc
    18121 taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat gaagcgattc
    18181 gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct tgtctgcact
    18241 gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg ctttgatgtt
    18301 aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg ttgcaatcgg
    18361 gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt tgattgactg
    18421 catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat ttcatacttt
    18481 tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca attgtctcat
    18541 tgtaatctct tacatattga gaaataatta aggattacag cctttacttt atcatgaaat
    18601 aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac gcaaaaaagc
    18661 taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt tttttattcc
    18721 tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg tccttcaact
    18781 agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga aataaaaaca
    18841 tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc atctagatat
    18901 tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa atctagatct
    18961 gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat gccggtttct
    19021 cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc aaattgcaga
    19081 cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt taccttttcc
    19141 tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag aagctggatc
    19201 agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt cggctggatt
    19261 tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat tccggctgct
    19321 tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa cacgacttct
    19381 tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg aacagttgtg
    19441 acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac ataagggaat
    19501 ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata aggattcaca
    19561 ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg atgagcatcg
    19621 atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct tcgcatggtc
    19681 accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa aaaaatgtct
    19741 tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta acgtgttaaa
    19801 ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat tggaggaatt
    19861 tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa acgattgaac
    19921 gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc ttgcgtaaac
    19981 ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat ggacgccctc
    20041 gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta gcacataaag
    20101 gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt aatgtaaagg
    20161 aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct tcttctagca
    20221 gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc cttttttata
    20281 tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca gtgcttcgat
    20341 acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat ccaaatggca
    20401 gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca ggataacgga
    20461 gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa gcttaggttt
    20521 agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt
    20581 attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga ctggagaaag
    20641 atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct tgatattctc
    20701 ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct aattgatctg
    20761 ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc acttttgttc
    20821 cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca cgcccaatcg
    20881 tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt ggccagccat
    20941 ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata tatgtttctc
    21001 catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca gaaaccagct
    21061 caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga ttaagtccgt
    21121 acattgctac tccataacga atcatattgc cgattgtttc atcatgccat aaagccgttg
    21181 cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc acttctataa
    21241 agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta gaaaaatggg
    21301 taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct gcctctttta
    21361 cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt agatgaatcg
    21421 aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc aaccattccc
    21481 gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg tcgtaaggaa
    21541 ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg actccttcat
    21601 ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca gctgtttcaa
    21661 tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt ccttgcggaa
    21721 gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc actgctttcg
    21781 ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc aaaataattt
    21841 gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct tcatgaggtg
    21901 aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct tgaaaggtaa
    21961 cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg taacgcccgc
    22021 ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt tcatcagagg
    22081 tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc ctaggaagtt
    22141 cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat aaatttagaa
    22201 cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa ataacaaaaa
    22261 tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta tttgaacgga
    22321 ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg ctgttattat
    22381 ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca cgacgattac
    22441 ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc attggcaatg
    22501 ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct gtcgttttta
    22561 gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct gctgtttgca
    22621 ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta
    22681 attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg
    22741 tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca
    22801 taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt tctggatctt
    22861 gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca cgtgcagcaa
    22921 cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat acgctcattc
    22981 gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt gcttctaatc
    23041 cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt aattcaggtg
    23101 tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt gcgcggacat
    23161 agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca tgatgtggat
    23221 ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct gagaaaagta
    23281 atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc aagaagccca
    23341 tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca agtttcaatg
    23401 tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata tgtggacgat
    23461 ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct tgtacacgta
    23521 cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct aattcacgtg
    23581 ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt tctaacattg
    23641 gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc
    23701 cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca aaaccagcgc
    23761 gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc aaatgaatcc
    23821 tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc
    23881 aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc ttagcccctg
    23941 tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg
    24001 ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg gcaaaaccag
    24061 tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc gttttctgca
    24121 agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat aagcccatgc
    24181 tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttg atagcatcaa
    24241 tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat ttttggttca
    24301 attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg aatttctcag
    24361 gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct aattccagca
    24421 tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa gtatctaata
    24481 ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct gcaccatttc
    24541 ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc ccttggcgta
    24601 tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta
    24661 cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca aaatcggttt
    24721 gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata ccgaatgttg
    24781 tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa
    24841 cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta gcgattcctt
    24901 ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct gcagcttttg
    24961 gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc atttccaaaa
    25021 cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca atattattgt
    25081 tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac gcggttagat
    25141 ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg cctacttttt
    25201 ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca ggaataactg
    25261 gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat gctccgtttg
    25321 tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga acgaataaac
    25381 ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc agtgcagagc
    25441 attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag atctctccta
    25501 aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact ctcacttcca
    25561 agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca atcactccag
    25621 cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct gatggtatcg
    25681 attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc ccatattttc
    25741 ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta attcattcag
    25801 gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa
    25861 catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt ctggcacttc
    25921 ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat catagaatgc
    25981 tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc ctaatacagc
    26041 aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat catactgata
    26101 ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa tgccgacact
    26161 agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt cacattgatc
    26221 aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat aaggaacttg
    26281 cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg cacatgcact
    26341 ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc cttggatcgt
    26401 tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt cttctttgat
    26461 ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac ttgggtccca
    26521 tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac ctttgaccca
    26581 ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat aaatggcatt
    26641 taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc cgccatatag
    26701 taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga tttactctca
    26761 tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg agccagcttc
    26821 taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt tttttataaa
    26881 aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg cagccgcttg
    26941 gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt acagcaaaaa
    27001 aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat caagatagga
    27061 cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac attcaaaaca
    27121 ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat cgaaacagaa
    27181 ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt tgcctctgct
    27241 tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt cgttaagatc
    27301 caacacccta acatcgccga agagatccga cttgatcttc aactatttga acgagcgatc
    27361 cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt actccaagaa
    27421 gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa tggcgaacaa
    27481 ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta tgacgctttt
    27541 tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa tcatttaatg
    27601 aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca gctgaaacaa
    27661 gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga tggttttttc
    27721 catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga agaacagaca
    27781 caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt tgcttttcga
    27841 gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat cgattttggt
    27901 atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt tttagcgctg
    27961 tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca gcaagaaggc
    28021 tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca gtattatgat
    28081 tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac gatttgtcat
    28141 caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt tgggacatta
    28201 gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc ttcgcctttt
    28261 gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca aagcggatta
    28321 gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca ccatctatta
    28381 gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca agacaaatta
    28441 ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt agctgcattg
    28501 atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga ggtcgtttcc
    28561 ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt ggcaatcgat
    28621 gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac agaaagaatc
    28681 agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt gtgaaaattc
    28741 ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt gaaaaagctg
    28801 ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt atcctgtttc
    28861 cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc
    28921 tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag acatatgatc
    28981 tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact
    29041 atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg
    29101 atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat
    29161 caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg
    29221 gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact
    29281 ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac
    29341 tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat
    29401 aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca
    29461 gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg
    29521 gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca
    29581 cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg
    29641 tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa
    29701 gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca
    29761 gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga
    29821 ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt
    29881 ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca
    29941 ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt
    30001 gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa
    30061 aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata
    30121 tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca
    30181 gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa
    30241 tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa
    30301 atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct
    30361 tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca
    30421 aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac
    30481 cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta
    30541 gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa
    30601 tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa
    30661 ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt
    30721 tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag
    30781 cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa aatacagaaa
    30841 gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt
    30901 ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat
    30961 tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc
    31021 gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc attttataat
    31081 cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt
    31141 catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc
    31201 tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt
    31261 gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca tttatctctt
    31321 cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag gacacacctt
    31381 ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg taatccactt
    31441 gtttttgacc aatgatcggc acaaaaactt ccggccatac ttcttgtatg actgccatca
    31501 gctcatccag ttcattttct gttactttta ttttttccat acacccacgt ccttttttac
    31561 attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta tacacaccca
    31621 ccgaccacgg tttcaagaaa atcacaatct gcataaattc cctaaaagat aatttcgtca
    31681 aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta
    31741 gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc tgatactttt
    31801 ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc aaaaatccga
    31861 ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca ttgccaaaca
    31921 tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc acttggatca
    31981 cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata aacgcttgca
    32041 aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac acaaagaaaa
    32101 aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat ccttttttag
    32161 acatctatat tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DAZUKR010000018        32157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000018 DAZUKR010000000
VERSION     DAZUKR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.018"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACG89C_000758"
     CDS             complement(407..766)
                     /locus_tag="ACG89C_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4899335.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACG89C_000759"
     CDS             complement(796..1137)
                     /locus_tag="ACG89C_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899336.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACG89C_000760"
     CDS             complement(1134..1808)
                     /locus_tag="ACG89C_000760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4899337.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG89C_000761"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG89C_000761"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4899338.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACG89C_000762"
     CDS             complement(4322..5512)
                     /locus_tag="ACG89C_000762"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4899339.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACG89C_000763"
     CDS             complement(5533..6060)
                     /locus_tag="ACG89C_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS4899340.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACG89C_000764"
     CDS             complement(6107..8896)
                     /locus_tag="ACG89C_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS4899341.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACG89C_000765"
     CDS             complement(9043..9243)
                     /locus_tag="ACG89C_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4899342.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACG89C_000766"
     CDS             complement(9696..10016)
                     /locus_tag="ACG89C_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899343.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACG89C_000767"
     CDS             complement(10519..11637)
                     /locus_tag="ACG89C_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899344.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACG89C_000768"
     CDS             11870..11992
                     /locus_tag="ACG89C_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899345.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACG89C_000769"
     CDS             12108..13415
                     /locus_tag="ACG89C_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS4899346.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACG89C_000770"
     CDS             13536..14735
                     /locus_tag="ACG89C_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4899347.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACG89C_000771"
     CDS             complement(14762..16030)
                     /locus_tag="ACG89C_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS4899348.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACG89C_000772"
     CDS             complement(16200..16841)
                     /locus_tag="ACG89C_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS4899349.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACG89C_000773"
     CDS             complement(17250..17753)
                     /locus_tag="ACG89C_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4899350.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACG89C_000774"
     CDS             complement(17876..18640)
                     /locus_tag="ACG89C_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS4899351.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACG89C_000775"
     CDS             18748..19023
                     /locus_tag="ACG89C_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4899352.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ACG89C_000776"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ACG89C_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4899353.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ACG89C_000777"
     CDS             complement(20579..20980)
                     /locus_tag="ACG89C_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4899354.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ACG89C_000778"
     CDS             complement(20994..21803)
                     /locus_tag="ACG89C_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4899355.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ACG89C_000779"
     CDS             complement(21790..22680)
                     /locus_tag="ACG89C_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4899356.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ACG89C_000780"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="ACG89C_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="ACG89C_000781"
     CDS             complement(23348..24289)
                     /locus_tag="ACG89C_000781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFS4899357.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACG89C_000782"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACG89C_000782"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4899358.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ACG89C_000783"
     CDS             complement(25274..26470)
                     /locus_tag="ACG89C_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4899359.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ACG89C_000784"
     CDS             complement(26488..27216)
                     /locus_tag="ACG89C_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4899360.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACG89C_000785"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACG89C_000785"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4899361.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ACG89C_000786"
     CDS             complement(28628..29086)
                     /locus_tag="ACG89C_000786"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4899362.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ACG89C_000787"
     CDS             complement(29083..30546)
                     /locus_tag="ACG89C_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4899363.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACG89C_000788"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACG89C_000788"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4899364.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32157
                     /locus_tag="ACG89C_000789"
     CDS             31602..>32157
                     /locus_tag="ACG89C_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4899365.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYV"
BASE COUNT         9379 a         6920 c         5511 g        10347 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtct
//
LOCUS       DAZUKR010000019        30527 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000019 DAZUKR010000000
VERSION     DAZUKR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.019"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..165)
                     /locus_tag="ACG89C_000790"
     CDS             complement(<1..165)
                     /locus_tag="ACG89C_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4899366.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLV"
     gene            complement(167..364)
                     /locus_tag="ACG89C_000791"
     CDS             complement(167..364)
                     /locus_tag="ACG89C_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4899367.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(758..2485)
                     /locus_tag="ACG89C_000792"
     CDS             complement(758..2485)
                     /locus_tag="ACG89C_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS4899368.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2478..3671)
                     /locus_tag="ACG89C_000793"
     CDS             complement(2478..3671)
                     /locus_tag="ACG89C_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4899369.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3858..4502)
                     /locus_tag="ACG89C_000794"
     CDS             complement(3858..4502)
                     /locus_tag="ACG89C_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4899370.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4596..4730)
                     /locus_tag="ACG89C_000795"
     CDS             complement(4596..4730)
                     /locus_tag="ACG89C_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS4899371.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4732..6864)
                     /gene="feoB"
                     /locus_tag="ACG89C_000796"
     CDS             complement(4732..6864)
                     /gene="feoB"
                     /locus_tag="ACG89C_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS4899372.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6861..7334)
                     /locus_tag="ACG89C_000797"
     CDS             complement(6861..7334)
                     /locus_tag="ACG89C_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS4899373.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7735..7959
                     /gene="nrdH"
                     /locus_tag="ACG89C_000798"
     CDS             7735..7959
                     /gene="nrdH"
                     /locus_tag="ACG89C_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4899374.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8118..10277
                     /gene="nrdE"
                     /locus_tag="ACG89C_000799"
     CDS             8118..10277
                     /gene="nrdE"
                     /locus_tag="ACG89C_000799"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4899375.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10327..11292
                     /gene="nrdF"
                     /locus_tag="ACG89C_000800"
     CDS             10327..11292
                     /gene="nrdF"
                     /locus_tag="ACG89C_000800"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4899376.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11381..12520)
                     /locus_tag="ACG89C_000801"
     CDS             complement(11381..12520)
                     /locus_tag="ACG89C_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4899377.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12829..13542)
                     /locus_tag="ACG89C_000802"
     CDS             complement(12829..13542)
                     /locus_tag="ACG89C_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4899378.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13796..14497)
                     /gene="nagB"
                     /locus_tag="ACG89C_000803"
     CDS             complement(13796..14497)
                     /gene="nagB"
                     /locus_tag="ACG89C_000803"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4899379.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14731..16263
                     /locus_tag="ACG89C_000804"
     CDS             14731..16263
                     /locus_tag="ACG89C_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4899380.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16454..17656)
                     /locus_tag="ACG89C_000805"
     CDS             complement(16454..17656)
                     /locus_tag="ACG89C_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4899381.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17738..18427)
                     /locus_tag="ACG89C_000806"
     CDS             complement(17738..18427)
                     /locus_tag="ACG89C_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4899382.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18533..18901)
                     /gene="rplL"
                     /locus_tag="ACG89C_000807"
     CDS             complement(18533..18901)
                     /gene="rplL"
                     /locus_tag="ACG89C_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4899383.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18954..19457)
                     /gene="rplJ"
                     /locus_tag="ACG89C_000808"
     CDS             complement(18954..19457)
                     /gene="rplJ"
                     /locus_tag="ACG89C_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4899384.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19661..19788)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19867..20556)
                     /gene="rplA"
                     /locus_tag="ACG89C_000809"
     CDS             complement(19867..20556)
                     /gene="rplA"
                     /locus_tag="ACG89C_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4899385.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20660..21082)
                     /gene="rplK"
                     /locus_tag="ACG89C_000810"
     CDS             complement(20660..21082)
                     /gene="rplK"
                     /locus_tag="ACG89C_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4899386.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21425..22093
                     /gene="sdaAB"
                     /locus_tag="ACG89C_000811"
     CDS             21425..22093
                     /gene="sdaAB"
                     /locus_tag="ACG89C_000811"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS4899387.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22106..22978
                     /gene="sdaAA"
                     /locus_tag="ACG89C_000812"
     CDS             22106..22978
                     /gene="sdaAA"
                     /locus_tag="ACG89C_000812"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS4899388.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23069..24169)
                     /locus_tag="ACG89C_000813"
                     /pseudo
     CDS             complement(23069..24169)
                     /locus_tag="ACG89C_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24261..25148)
                     /locus_tag="ACG89C_000814"
     CDS             complement(24261..25148)
                     /locus_tag="ACG89C_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4899389.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25225..25770)
                     /gene="nusG"
                     /locus_tag="ACG89C_000815"
     CDS             complement(25225..25770)
                     /gene="nusG"
                     /locus_tag="ACG89C_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4899390.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25871..26041)
                     /gene="secE"
                     /locus_tag="ACG89C_000816"
     CDS             complement(25871..26041)
                     /gene="secE"
                     /locus_tag="ACG89C_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4899391.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26065..26217)
                     /gene="rpmG"
                     /locus_tag="ACG89C_000817"
     CDS             complement(26065..26217)
                     /gene="rpmG"
                     /locus_tag="ACG89C_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4899392.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26379..27023
                     /gene="cbpA"
                     /locus_tag="ACG89C_000818"
     CDS             26379..27023
                     /gene="cbpA"
                     /locus_tag="ACG89C_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS4899393.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27079..27582)
                     /locus_tag="ACG89C_000819"
     CDS             complement(27079..27582)
                     /locus_tag="ACG89C_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4899394.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27595..27641)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28179..29144
                     /locus_tag="ACG89C_000820"
     CDS             28179..29144
                     /locus_tag="ACG89C_000820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4899395.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29243..30415)
                     /locus_tag="ACG89C_000821"
     CDS             complement(29243..30415)
                     /locus_tag="ACG89C_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899396.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30402..>30527)
                     /locus_tag="ACG89C_000822"
     CDS             complement(30402..>30527)
                     /locus_tag="ACG89C_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899397.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9313 a         6271 c         5406 g         9537 t
ORIGIN      
        1 caccaatcta tttattaaag gaccgggatc taattcatat ttttgttttt ctaattttaa
       61 atataccttt tgtaaaacct caagaatatc agaatattta ggatctttac ttatagatat
      121 atcattatat aattcatgaa caatttgctt agcatttttt ttcattttag cacttccctg
      181 gaattgctcc acctaaaaaa ccacctatag acattcctgc aatacaagta gttgccttgg
      241 cccaatcgac cgtacattta tttttagtgc aatacactcc atttccataa tattttccac
      301 tatgagtggt acccccatag ataagttgtg tctctttaat agacaaaatt tttaaatgtt
      361 tcataatcta atcaccccta aattaatgtt tttagactat tattctaaca ttcattaaat
      421 ttcaaaaaac aattccttct tgaaacgtag caaaataatt cctgaataag aatttaatca
      481 cttttaagta tataaactat gtatattctt taatattttt tgagaatgca cttcgtacat
      541 aataaacttt aatcacatta gtctttcata cctacgatcg atctctttga ctcgcaccca
      601 taaattcata atagtttgtc ggcgtctttg gacgtttcga agatgagcta cgcattctct
      661 ttttttatag acaaaaaaga tagagacgaa catggagctt ccaaatttgg atatttgatc
      721 caattttgga agctccattt atttcgtgtc tgtcttttta ttttttccag ctttcgacta
      781 attcttggtt ctctttgatc cagtcttttg ctgcttgctg tggatctttt ccattgttga
      841 tttccaacat aactttttcc atatcttttt ctgaccaatg gaaattgtct aatactttgt
      901 aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat cgtatggatc tgctcttcgc
      961 ttcccattcc gttttcagga tcttctaagt atttcagatc atatttggca aacatccaat
     1021 gaggtgtcca tcctgtaact acaattggtt cgtgtttttt gatcgcttga cctagtgcaa
     1081 cggtcattgc tcctgaagaa gatgtctcta ctttccagtc tttcaagttg tcatattttt
     1141 ggatcgtgtt ttcagcagcc gtaactacac cagctcctgg ctcgattcct atgattttct
     1201 tacctgcctg atcggttaac tcatcgatcg aatttacatc catgtaggct ggcactgcta
     1261 aaccaacttt tgctcctgtc aaattggcac ctaggtcttc gacttgatct ttatattgtg
     1321 catattgtga accatgagtt ttaggtagcc aagctgaaac catggcatct gactcgccat
     1381 ttgaaacaga tttccacatg attgaattat ctaatggcgt catcgttaca tcatagccca
     1441 tttgtttcaa tacttcaccg actacatttg ttgaagcaac ttctgtatcc cattcaacat
     1501 aagataagtt gatacgtttt gtttctttgg cagatgaaaa actccctata ccaatcgcac
     1561 ctaagatcac tactactaca gcaccaatga tcagtccttg acgtttttta cttttctgtg
     1621 ttgttgctcc tgcagctttt ttattgtttg gctggttcaa atgctgtgta aaacggtcaa
     1681 tgatgattgc taagataact aatgcaaccc cgttgacaaa tccattcccg acttgtgctc
     1741 tttgcaatgc tgataatacg ccgcgtccta agccgggtgc accgatcata gaggcaataa
     1801 cgaccattga aagtgacagc attgttgttt gattgatccc tgccatgatc gtacttttag
     1861 ctaaaggcaa ttctagtttg aatagttttt gacgacttgt gctcccgaat gaatcagaag
     1921 cttctactaa ttctttagga acttgtcgaa tccctaaatt cgtaaaacga acggttggtg
     1981 gcagagcaaa gatgactgag gcaaataccc ctggaaccat cccgataccg aaaaatgcaa
     2041 ctgctggaat caaataaacg aatcctggca ttgtctgcat aaagtcaagg atcggtttga
     2101 taatactttg ggctttactg ctttttgcca tcaagatccc taatggtacc ccaatgatga
     2161 tcgagatcac actagataaa agaacaagtg tgacagtatt catcaaatca ttccatagac
     2221 cttggttata aataaaccat agaccgatta atgtgaacaa cgtaagtcct ggacgtttct
     2281 tagaaataaa gaaagctgct accgtcaata acaagataaa caaaggagct ggaatcacta
     2341 acagtaaatt tgtaatcccc gacatcactg cctgtccaat cgtttgtaaa aatgaaaata
     2401 ggccagaaaa agttgtcgta aaccattctg tgatattttc tacccagttt gccactggta
     2461 attgatagtt caatagatta ttcatgttca ctcacctcgc ttgtttctgc taatgcttca
     2521 atcacactac ctcggatgac gacacctttt aattttccat cctctacaac tgcaagtgga
     2581 gtcggtgagt cataaataat attgaaaata tcacttacca ccatgtcttt cccaatcacc
     2641 gtcacatttt catctaggaa gtcgatcaat ggacgttttt gcttcctagc ttctaacgct
     2701 tgatctgcag tgatgatccc tttcaggtgt ctctttctgt caacagccat cagcatgctg
     2761 acttcttcat tacgcatcct tgtcaatgca acgtttggtc catcagattc gatattcgtc
     2821 gtcaatgcag gtaccatgat gttttgagcc gtaagcactt tggaacgatc gacttcttcc
     2881 acaaattcac gtacataatc atttgctgga ttggtcaata tttcttcacc tgttccgatc
     2941 tgcatgattt cgccgtcttt catcaaggcg attctgtccc caatccgtaa agcttcattc
     3001 aaatcatggg taatgaaaat aatcgttttt tgaacattgg cttgcaggtc cagtaattcg
     3061 tcttgcattt ctcgccggat cagcggatca agagccgaga acgcttcgtc catcagcaag
     3121 atctcaggat catttgctaa agctctggct aggccaaccc gttgttgcat cccaccagat
     3181 aactgactag gatattggtc tttaaatgaa agcaaactag aattttctaa tgctttttct
     3241 gctttttctt gtcgttcttc tttaggtaca ccacggactt ctaacccgta ttctgtattt
     3301 tccaagatcg ttcgatgtgg aaacagaccg aagttttgga aaaccatatt gattttatga
     3361 cgacggactt ctcgcaattc ttctttgctt aacttcgata catcttctcc atcaatatag
     3421 atactgcccg aagttggctc aatcagacga ttcaatagac ggatcaatgt ggatttccca
     3481 ctcccggaaa gccccatgat cacgaagatt tctccttctt ttacgtcaaa attcacgtca
     3541 tacacaccaa ccgttgcacc ggttttcttt aaaatctccg ttttcggctg gtgatcattc
     3601 atcatatcta aagcggcttg tgtcttttta ccaaatatct ttgttaaatg ttttacttct
     3661 acttttacca aacaaattct ccttttttat gtaaattcat ttttctttct gaaaaaagtg
     3721 accacactat gttaacatca tgtagtcact ttgtcaaaaa tgaaagaaat ttgcttgtcc
     3781 aaactgaaaa aaacttggct aaatctttgt aaataaaagg tttagccaag ttaatatttt
     3841 tttaatattt cacacaatca ttctgcaagc tgatgctccg ggtaaaaaaa gtttgtcata
     3901 cgctgtagtg tatattcatt tccaataaaa tgaacgatgt cccctgccgt aaatttagca
     3961 taggggccag gtgaaacaag cagttcttca ttatgagaaa ttgcgataac tgtggcgcca
     4021 gtcgcctgcc agatattcaa ctcgctaatt gaacggtcca gatgcattgc tttttctgat
     4081 aaagataatt caaacggaag taaaggattc atcttatgta ttcgcttcgt ttgattgacc
     4141 agctgatcca ataattgaga aaaatgattc agctcatctt gttgcttctc cacacttgcc
     4201 atgatttctg cttttgtatc ttggatggat tgtacatctt gatattgcaa taggaaatct
     4261 tttgcttttt ccttcgaagc aacaaatgcc ccacttccat gccgtacttc catgattcca
     4321 agatctacca gcacattgat agccttgcgg gtagtctcag gagaagcatt gtacgtactg
     4381 gcaagtgttg agcgggcatg gatcttctca cccactttat atcggttttc aacgatacgt
     4441 tcggctaaat caacagcaat ctgctggtag cgcgggcgga cgattttttt ctttccagac
     4501 attttctttc ctctttcttt ttttactttc cctcatctta ctatgctcta gcaaaataag
     4561 caaaaaaggg gagaatagcc tcttgcaacg actgatcatc gcttgatctg ttcctttttt
     4621 actgggcaag ctgtttgaca gtcatcacaa ttcttaccgg attttaaacg agtatagaca
     4681 attgcgcctg cacttccaaa gattaacaca cttaataaga atgtcgccat cctattcctc
     4741 tccttctaat gacaatatag tataaaactg gttcttggat tttggctgac ggaacaaaac
     4801 ataaacgagt cctatcatca gtaaaatagc aagatacgtc cctaacgcta cctgtccacc
     4861 ctccaataag tgtcccaact gataaatcac aaaactaact aaataagcta aaccgcattg
     4921 atagcctatg gcgatccaag tccatttctt ggcattcatt tcccgatgga tagccccaat
     4981 tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac aatgaataag ctgctgcggg
     5041 agtatatgtg tgttgtagca cttgccagat ttctctgcca ttttcggata cttccgcatg
     5101 accaaacaaa ataccaaatg tgccaataat attttcttta gcgaccaatc ctgtaatcgc
     5161 tgcgactgtt ccttgccagt ttccccaacc aagcggctga aaaacaactg ccaggattcg
     5221 cccgaaaaag gctaagatac tttggtcttc agaaacaggt tgagcataaa aattataaga
     5281 agagctgaac cagatcacga tactagaaac aaagataatc gttcctgctt ttttaacaaa
     5341 agacttgctg cggtcatagg tttgtcttag gaccgctgac cattgaggaa aatgatatgc
     5401 gggcaattcc atgataaaag gagctgtatc tccagcaaac aaacgtgttt ttttcaagct
     5461 gattccagag aaaataattg cagtcatacc aagaaaatag gcagaaggag ctacccaaga
     5521 ttgatgagga aaaaaggccc ctgctactaa ggcaataatc ggcaactttg cagaacaagg
     5581 catgaaggtt gtgaccatga tcgtcaaacg acgatcttgc tcgttttcaa tcgtgcgact
     5641 ggccatgacg cctggcactc cacatcctgt agcaatcagc ataggaatga aggattttcc
     5701 tgaaagaccg aatttcctga aaagtcgatc catgacaaaa gcgatcctcg ccatatatcc
     5761 gcagtcttcc agaaagctca agcataaaaa caatataatc aattgcggta gaaatcctaa
     5821 cactgctccc acgcctgcga tgatgccgtt caagatcagc tcctgcatcc atcccgctac
     5881 ttgccattca gctaacaatc tgccaacatg ttctggtaac catgtaccaa acaatgtgtc
     5941 attgatccaa tctgtcccca ttgttcctac tgtttgaata gccagataat agatcagcca
     6001 catcacaaat gcgaagatcg gcaaggctaa ccaacgattc gttgcaatct gatcgatttt
     6061 gtcactcatc gataattgga aagaatcatt tgacacaaca cagagtgtac atagttgggt
     6121 aataaattca taacgttcat tgaccagaat cgtctcacta tcgtcgtcca atagtttctc
     6181 tgttaactgg atcagttgct cgatctcttt ttgctgagaa actgctatct cgtaatcctc
     6241 tcttaccttc tgatctcgtt caaaatattt cattgcatac cagcgtttct tgccgctttg
     6301 ttctcctagt acatcttcaa tttcatgtaa agctgcttct aaacgtggat catatgtaca
     6361 gggaacagga atagaaggcc gcttttttgc agactcctga atcttttcga ttagtttttc
     6421 gattccttgc tttttcaata cgctgattgg taaaactggt acaccaagac cataggaaag
     6481 cttttctaga ttgatttttt ttccttcttt tttgatgaca tccatcatat tcagaccaat
     6541 gatcatagga atgtctaaat ccaataactg cgtagttaaa taaagatttc gttctaaatt
     6601 ggtagcatca ataatgttga tgatcagttc aggctgttcc tgaaccaaat aatctcttgc
     6661 aatcacttct tctggtgtat aaggagacaa agaatagatg ccgggtaggt cctgaatatg
     6721 aatgttcgga ttttttttca acgatccttc ttttttttct actgttacgc ccggccaatt
     6781 ccctacatat tgattcgaac cagtcaagag attgaacaag ctagttttcc cgctgtttgg
     6841 atttccgact aacgcgatat tcattctttt tcctcccttt gtatcaagat tagttcggcc
     6901 tctgactttc gtaacgtcag ttcataccct cgcactgtca gttcgattgg atctcccaga
     6961 ggggctagtt ttcggataac tatttttaca tttcgcgtca atcccatatc cattaaccgt
     7021 cgcttcactg cgccttgtcc gtggattgct acgacgatcc cagtctctcc tgcttttaat
     7081 tgatcaagag aagtccagct ttcagaggaa gcagtctttt tattgacaaa gatttgacct
     7141 agtatttccc gacttactgc cactcgatga ctttttacca gtaaaatggc attgttgcct
     7201 gataaattga cgacgacgat tttacttccg gcaacgatcc cgatattttg caaatggcgt
     7261 tttgtttttt cttctgtttc gattcgttcg acttgataaa cgaaattctg ctttgcctgc
     7321 gctaaggtct gcatattgat tcctctcttg tgaaataaat catgtattaa aatcattcat
     7381 tatatgttct aattttctta cgtgttacgc aaataaatga gaatcattct cacatagaat
     7441 actctcggta attgagaatg tcaattatac ttgtgatatt atgagcgaat ctttagtatt
     7501 gatagataaa aatgagaaaa aaatatttct tcatagtttt ccacaatttt ttctctatta
     7561 aatttgctat tttcccacac gatacttgtt ttcagctctt ttttcaaaaa cacaaaactt
     7621 agtattccac agtttccaca acacaatatg tagtgtttgt tgtttgactt ttttactaag
     7681 agttttatac tatctattat agataataag aatgtgttag aggaggcact caccatgaac
     7741 gtaaaaatct tttctaaaaa taattgtatg caatgtaaga tggtcaaacg gtttttaaca
     7801 gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac agcctgaagc agtagactgg
     7861 ttgaaagcac aaggattcca aagtgttcct gtcattactt ctgatgcaga aacagttatc
     7921 ggattccgtc ctgatcagtt gcgtaaactt gccagctaac atgactaaaa gaatcaaaac
     7981 gtgatacatg gatttgaagg gcttgagaca gcaccagttt ttgtcgcaaa cttccaaatc
     8041 cttttttaaa gcttaaaaac aatgcaatga tttgcaatca agcatcatca aatacgaaat
     8101 gaaggttggg cgtacgtatg agtcttaaaa atttacaaga tgtcagttat ttcaagctga
     8161 ataatgaaat caatcggcca gtcaatggtc aaatcccttt gaataaggat aaagaagctt
     8221 tagttgcctt ttttgaggaa aatgtcaaac caaatacgat gacttttcta tctattatgg
     8281 aaaaaatcaa ttacttgatc gatcaagaat atatagaagc agaattcatt caattgtatt
     8341 caccatcatt tattgaaaag ctatatcaat ttttggacga gcaacatttt tctttcaaat
     8401 cttttatggc tgcttacaaa ttttattctc aatatgcgtt gaaaaccaac gacggttccg
     8461 cttatctgga aacttatgaa gatcgagtag ccttcaatgc gctttacttt gcgaacgggg
     8521 atgaagaatt agcacttgct cttgcagacg aaatgatcca tcaacgatat cagccagcta
     8581 caccttcttt cttgaatgct ggacgtaaac gtcggggcga attggtgtct tgtttcttga
     8641 ctcaagtcac tgacgatatg aactctatcg ggcgctcaat caactctgct ttgcaattgt
     8701 cccgtattgg tggcggcgta gggattactt tatcaaatct tcgtgaagcc ggcgctccta
     8761 tcaaaggata tgagggtgct gcaagtggtg tcgttccagt catgaaatta ttcgaagata
     8821 gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc tggcgtggtt tatttgaacg
     8881 ttttccatcc agacattatt gctttccttt cagcaaaaaa agaaaatgca gatgaaaaga
     8941 ttcgcgtaaa aactctttct cttggcgtga tcgtacctga taaattttat gaattgacac
     9001 gtaaaaacga agatatgtat ttatttagtc catatagtgt cgaaaaagaa tatggtgtcc
     9061 cattctctta tgtaaatatt acagctgagt atgatcatct agttgcaaat ccaaacattc
     9121 gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat ctcaaaatta caacaagaat
     9181 ctggctatcc ttacgtaatc aatattgata cagcgaaccg cgagaatcca atcgatggca
     9241 agatcatcat gagtaattta tgttcagaga ttttacaagt tcaaacgcct tctgtgatta
     9301 acggcaaaca ggaatatgaa gtattaggaa cggatatctc atgtaactta ggctcgacaa
     9361 atatcgtgaa tttgatgcaa agccctaact ttggtcaatc tgtgcgtacg atgactcgtg
     9421 cattgacatt tattacagat gcatctgata tcgatgtcgt accaccgatc caaaacggaa
     9481 ataagctgaa tcatacgatt ggattaggtg ccatgggctt acacacttat cttgcaaaag
     9541 aacaaatgga atatggctca gaggattcat tagactttac aaatatttac ttcatgctgt
     9601 tgaattattg gacacttgtt gaaagcaaca atatcgctcg tgaaagaaaa caagttttcc
     9661 ataattttga aaaatcagct tatgctgacg gcacttattt cgaaaaatac gtaactggag
     9721 aattccagcc aaaatcagaa aaagtcaaag cattgtttga aggcattttt atcccaactg
     9781 ctgaagattg gaatgctttg aaacaagcag taatgaaaga tggactgtat catcaaaatc
     9841 gcttggcagt agcaccaaat ggctcaattt catatatcaa cgatacgagt gcaagtattc
     9901 atcctatcac tcgcatgatc gaagaacgcc aagagaagaa aatcggtaaa atttattacc
     9961 cagcgcctta tctatccaat gagacgattc cgtattacac ttctgcttat gatatggaca
    10021 tgcgtaaagt aatcgatgtt tatgcaatgg cacaacaaca cgtcgatcaa ggcatgagtt
    10081 tgactttatt catgcgttct gaaatcccag aaggcattta tgaatggaag acaacaacga
    10141 agcaaacgac acgtgatttg aatattttaa gacattacgc tttccacaaa ggaatcaaat
    10201 ccatttatta cgtacgtacc tttactgatg atgcagaaga aatcggaagc aatcaatgtg
    10261 aaagctgcgt gatctaatat catagataga ttcgcttatt catattttaa ttaaaggagc
    10321 attctcatgt cagaaacata ttatgaagca ataaactgga atgaaattga agatatcatt
    10381 gataaatcca cttgggagaa gctgacagag caattttggc tagatacacg tatcccactt
    10441 tcaaatgacc tagatgattg gcgtacattg tctcaattgg aaaaagatac agtaggacat
    10501 gtatttggcg gtttgacact gctagatact gtccaatctg aaagtggcat ggatcagttg
    10561 cgtaaagatg tccgcactcc ccatgaagaa gccgttttga acaatattca attcatggaa
    10621 tccgttcatg ccaaaagtta ttcttctatt tttagtacac tgaatacgaa aaaagaaatc
    10681 gaagaaattt ttgactggac aaatacgaat ccttatctgc aaaaaaaagc tgagcgtatc
    10741 aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa ttgccagcgt ctttttagaa
    10801 acattcttgt tctattcagg tttttatact cctctttatt atttaggaaa caacaaattg
    10861 gctaacgtag cagagatcat caaactaatc atccgagatg aatctgtaca tggaacctac
    10921 atcggttaca aattccaatt aggattcaac gagttacctg aagcagaaca agaaacgtta
    10981 aaagactgga tgtacaatct tctttatgag ctttacgaaa acgaagaacg ttatacagaa
    11041 gaattatacg atccaatcgg ctggacagaa gaagtcaaga cattcctacg ctataatgcc
    11101 aacaaagcat tgatgaacct agggatggat ccactctttc cagatactgc caatgatgtc
    11161 aacccaatcg taatgaacgg gatttcaaca ggcacaagca accatgactt cttctcacaa
    11221 gtcggaaacg gctatttgtt aggtcatgta gaagcgatga aagacgacga ttatttgatt
    11281 ggattggaat aaggatttat tttaattcaa aaatttttcg aaaaataaaa agagtttagc
    11341 gtacagatat tttaaatgta cactgaactc ttttttatgc ttattcactt gtttcttcca
    11401 gcacttttgg actgtctaac ttttccgaaa catgttccat tcttctcaat aaatagattt
    11461 tccggatatt taatattatc gtcttaatca ctgcatagac aggtatgcaa atcagcatgc
    11521 ctaatattcc tgcgacactt cctgctccaa tcaataccaa gatgatcgtc aaaggatgga
    11581 tattcattga tttcccaaat aacaacggct tgatcaaatt tccatctaat tgttggatca
    11641 ctaaaatcga caacgccata tacaaagctt gaaatggaga gataaacaaa ccgacaatga
    11701 ttgctggtac cgctccaata aaagggccga catacgggat aatattcgtg attccgcaaa
    11761 tgaccgcaag caacaagcta taaggctgat cgaatattct cattgccaaa aagctcatta
    11821 caccaataat caaagcatct aagatcgtac tgctgatata ggcagataat gtttcgttta
    11881 attcttttac cgtttggcgt aactctttac gaatcgcttt cggaaagaag tgagacaacg
    11941 catcaataaa tttgtgccca tccttgaaca taaatagcaa aataaatgga acggtaaata
    12001 acaaaataaa aaattgcatc ataaatgaaa tgatttttga gacacttgca gataaactga
    12061 ctaatatgat attgaacaaa ttactaagag ataaatttgc tgatgctaat tgttcttcca
    12121 aattgaaatt tttaaactct tcccgctgcg ccaattcatt taaccatttg cttgtttcat
    12181 ctgcatacat tggcaagctc tgggtcaatt gaattgtctg ttccattaac tgaggcacta
    12241 cattcatgac agttaaaaca actaaaatga ttacgaccaa aaacgtcaat aaaaaaccga
    12301 acactctcgg gatccgatgc ttttcgagaa aaacaacgat tggatcgaac atataataga
    12361 taaaaccagc aacaagcaat ggtaaaaaaa ctgccgaaag cattttcaat attggactaa
    12421 aaatattcgg catttgtaaa ataaagtaca cgccgatgat cgtgagtact agctccactg
    12481 tccagaaaaa taattttgat tgtttaaaac gtgattgcat acatctcctc caaggtcctt
    12541 tgatacttct attctatcat ttaacaaaga gttacgcgct gagattttca tttgcttaaa
    12601 aaaaacaaaa aaaataaaca tattttaatg agtagacgag tacttaaaca aaaacacggt
    12661 ttggagtagt gacaaaaatt atactgcagt ccctttttcc aaataaacgg tgttcaaaag
    12721 cttgcatctt cgtaataagc ccagccaaac gaaaaatata aggaactatt tttatttggc
    12781 tgggcttatt acttgatgta ggacatctat tttacaacct cagatcattt attgcgcaat
    12841 actgactaaa tgcaactttg tgatattttt gtgtacggac aactcctcga tcacatcggc
    12901 ataagtgagc ccttttggaa gatcaatcgt atagatattt ttgtacactc ggtagtcatc
    12961 aacgaatttt acgtcaaaat tcacatcttc gattacgata tttttctctt caaaataatc
    13021 ggtaagaaaa tctttggttt cttttctatg gataaattgg atttctaact ttttagtcgt
    13081 aggtacatgg atcacgcgtt tcactactgt gagtgcaata gcaatcccga taaaactgat
    13141 gatcgcaatg gaatagtaac ccatgccgat ggcgatccct aatccagcaa ttgcccataa
    13201 agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg acgatgatcg ttcccgcgcc
    13261 taagaaaccg acaccactca ccacttgagc aatcagcctt gcttcatctg aacgaatcgt
    13321 tccagcaagc tgtggatact ctaaagcatc tcttaatgca ccactagcga tctcaacttg
    13381 gatcaatgca atgatacatg ctcccataca aactaaaata tgcgtacgca tacctgctgg
    13441 acgattttta tattgtcgtt caaaaccgat tgctccgcca aatagcatag cgatccctaa
    13501 acgtaaaata atctctggaa ttgtcaatac catatcattc atctaaacac cctccagtcc
    13561 aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa tttttacctc ttacgaaaaa
    13621 aggactgtga caaaattttt gtcatagtcc tttttatgaa taaacggtgt tcaatcagct
    13681 gaccctcggt attactcaac aaacggcaaa atatggggag ctatttttcc tttgtcttct
    13741 ctaatactca ggtcaaaacg ctgatttcac aacctcttta gcctactatt gaaaattaca
    13801 acttacttgc tgcttcttca tcgatgatca caacaacatt gtcgtgattt tgcaatacac
    13861 ttgctggcaa atgattcgtt acaggtcctt caatcatccc tttgattgca tcagctttgc
    13921 tttcgccaaa tgccatcaag acgatttctt tacttttcat aattgaaccg attcccattg
    13981 aaacagcttt tgttggtaca tcttctgctt tatcgaagaa tcgtttgttt gcattgattg
    14041 ttgattctgt taagtcaact actgaagtcg ttccgtcaaa tgaagcacca ggttcattga
    14101 aaccgatatg tccattttgc ccgattccta agatttggat atcgattgga tgtgcatcga
    14161 tgatttgatc atactttttg catgctgctt ccagatcttc tgcttttcca tctggtacaa
    14221 acgtttcttt gaatggtttt ttatcaaata attggacatc catgaaatga cggtagcttt
    14281 gttcatctga accgcctagt ccaacgtact catctaagtt tacagaagtc atttcactga
    14341 aatccaaatc gcttgaagtc atttctttat ataaagtaac tggtgtgctt cctgttgcca
    14401 atcctaatac ttttgcacct ttgttcatac cttctttgat gatttcaaat gctttttttc
    14461 cgccttcttc agcgtttttt acacgaatga tttccatagt gattgctcct ttatattttg
    14521 gtatagccca attatagcat tctttttttc tttttacaat aattagtata gaccaaattg
    14581 catttttttc attttcttga caataaatag gaatccgtgt taggtttcat aagatggttc
    14641 aatcaaacaa tccatgttgc atgaaaacca agcaagtgat tcactgtgga cgctttatgt
    14701 ctgcagtttt tttatgaaaa aggagttatt atgagtatac tgacattaaa caacattacc
    14761 cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc tgcaattaaa tgctggcgaa
    14821 catcttgggc tgattggaca aaacggagcc ggtaaaagta cattgattaa aatcatcact
    14881 ggtgagatct taccagatga cggacagatc cattggcaaa aaaatattca caaaggttac
    14941 ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg aagaatttct aaaaactgcg
    15001 tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc tttaccaaac atatagcgag
    15061 accttcgatg attccttgct ggaaaaagct gggaaactcc aaacggaatt agaccaagga
    15121 gtgttttatc aaatcgacac cttagttgct gagatgagca gcgggttggg gatcgatgtc
    15181 ttaggtttag attcactgtt gaaaaatcta agtggtggac aacgttcaaa ggtgatttta
    15241 gcaaaactac ttttagaaaa cccagatgtt ctgatcttag atgaaccaac caatcactta
    15301 gatgatcagc atatccaatg gctgacgcag tttcttcagg attttgacgg tgcgtatatc
    15361 gtgatttccc atgaccaaga atttcttgat cagatcacca cccatatcgc agacatcgag
    15421 tttggaaaga ttactaagta tactggccat ctaaaacaag cattgaaaca aaaggaacaa
    15481 aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa aacaaatcga aaagaccgaa
    15541 gcttatattc gaaaatacaa agccggttcc cgatcaacga tggcaaaaag ccgacaaaaa
    15601 caattagata aaatcgaacg gctgacgcct ccagcttcag caagtaaagc tgtctttgat
    15661 tttccattct cgcctattgt cacgacatta gcagtggaaa caactgattt agtcatcgga
    15721 tatgatcgac cgctacttgc acccatcaat ttgaccatcc gctttggtga aaatattgcc
    15781 attcgaggct tcaacgggat tggaaaatct actttattaa aaacactgat cggtgagatt
    15841 gaaaaaataa gtggcgattt ccacttccca gataacacaa aaatcaatta cttttcacaa
    15901 gatctgtact gggaaaatcc attagaaaca ccactacaat atttaagcag tcaatttcca
    15961 aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag cagggttagt taatcaatta
    16021 gcaagtgaac ctctcactac attaagtggt ggggaacaaa ctaaggtgaa acttgcacag
    16081 ttgacaatga accaaggtaa tttactgatt ctcgatgaac cgacgaacca tatcgatcaa
    16141 gaaacgaaag aaagcttaca agacgggata cagaaatttc caggaacagt aatcatcgtc
    16201 tctcatgaac aagaatttta tcaagatctt gtgcaacgag tcattgagat tgaaccaaaa
    16261 taaacgtttg attgaatagt tgaaaaaaag gagctgtgac aaaagttttg tcacaactcc
    16321 ttcttctgaa taaatggtgg ccaattagct gaccttcggt attagctcaa aaaacggcaa
    16381 aatatgagga gctattttta tttggctatt cttattactt gatgcagaac agcttttcac
    16441 aacctctttt cgtctatttt ttattttcga gatttctctt tcctttaaaa acaactgttt
    16501 gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac cgttacacct aaccagccat
    16561 actgctgcca cattgtcgtc cctagcaatg aaccagccgc acctccaata aagtacataa
    16621 acataaatac agtattattt cggttgcttg ctgcttcacc tagcccttgt actcgtgttt
    16681 gattggcgac ttgaccaaat tgattcccga cgtctaataa aataatagct aaaagcaaaa
    16741 tggccaaatg gcttccgcca accaataaag caataaagct tattgtttgc ataacaagtc
    16801 ccatatacac gatttttctt tcagaataag aatctgccag tcgtcccacg ataggtgcag
    16861 caactgctcc agacaaacca aaaatcgcta atatccctgc ttcgaatgtt ccccaattgt
    16921 aggcgggact gctgatataa aaaatcaacg tagaccaaaa gatcgaaaat gttccaaaca
    16981 tgaaaaatcc actgactgcc gcttctctta atagacgttg agaagcaact aaatgcggca
    17041 aacttttaag tgaatcaagg tatgaaacag ctgtactact ttgctggaca gaacgcggca
    17101 ttttccagta aagcagaagt gtaagaattc cgactgcaca taaagcaaat aaatagatca
    17161 tccgccaaga aaaaagactg gcaacaatac cagataccgt ccgagaaagc aaaatcccca
    17221 cgagtaaacc gctaagcaat gtacccatga ccttccctct tgcttttggt cccgctagta
    17281 ccgcaccgta aggaatgatg atttgcggca cgatcgaaag taatcctatt aaaaaagaag
    17341 cgcatgcaaa taacgtaaac gaaggcgcaa aaaaagcggc caacagagag acggcagata
    17401 acgctgccat tcgaatgatc aattttggac gatccaccac gtcccctaaa ggaactaaaa
    17461 acagtaaccc taacgcatag cctaattggg tcaacatcgt caatatcccg actgcacttg
    17521 tgctaacaga taaactagct gctacttttg ttccaatcgg ctggatgtaa tacatattgg
    17581 caacaacaac gccacaagtg attgataata gtaaaataag cgaagttgtt aaaggttttt
    17641 tttcattact cttcattgat ttcccccatt tcctatctta gtttacaact tatggaaaag
    17701 attggaaata gaaaaataca actattcgcc acacttatta tttgtgcttt tgttttcctt
    17761 gttttttctt gcgatcttta taaatgaaga caactaaccc tactagcaca gtaacacttg
    17821 caaccaacgt gactgcagtt ttgttatatc cgtgaaacag agcttctcca ccacacgcat
    17881 acaacacaga cgaagggatg actccaatca cgatcgacaa tatgatttgc tgtagaggca
    17941 atttcatcgc atctgcagca aaattgacca tagaagatgg aataaccggt atcatatagc
    18001 ctaacattac acctattttt ggatgtttca tttgtcggat tgcttgcacc caatgattcg
    18061 tttcggtttt cttatctaaa aattttaagc gatgcataag gaaaattgca gtgatgttac
    18121 caagagcatt acccaaaatg ttgatgatcg cacctgtcag tggtccaaag ctcaaaccag
    18181 caatgactcc aactactgaa gtaggaatac ctggaacagc acacatcacc gctgtcaata
    18241 cgatcaagag tatcgatgca aaagtcccat gcgaacgaat atgtgcgagt agctctgcct
    18301 tactcgctct cggatttaaa aataattgga tgtctgtctg atattctaaa tacaaccgat
    18361 agcccactac cagtatcaga atgattccca acgagatcaa tatcaatcgg attattttct
    18421 ttttcatatt tcctcctgaa aaaaatctat attcattaaa taatagttta acataaaaac
    18481 aaaaaagcac cgaaatccaa cataggatct cagtgctttt aaatagtaaa aattatttaa
    18541 cagttacaga tgcgccaact tcttcaagtt tagctttcaa ttcttcagct tcttctttag
    18601 atacgccttc tttaactggt gcaggagcgc catcaactac agctttagct tctttcaagc
    18661 ctaaaccagt tgcttcacga actgctttga taactttaac tttttggtct ccagctgcag
    18721 ttaattctac agtgaattct gtttgttctt cagcagcagc agcgccacca gcagcagcta
    18781 cagctacagg agcagcagca gatacgccaa attcttcttc aatagcttta actaggtcgt
    18841 taagttctag gatagttgct tcttttaact cagcaacgat gttttcaatg ttcaatgcca
    18901 ttataatttc ctccgtttaa ttgttctttt ttatttaatg ctttatgcgg caattaagct
    18961 gcatctccat cttctttgtc tgccactgct ttgacagcgt aagccacgtt gcggactggc
    19021 gcttgtagta cagatagcaa catagataga agtccttcgc gatttggcaa ttttgccaat
    19081 gctgtgattt cttccaatga agagacttta ccttcgatga taccgccttt gatttctaat
    19141 gcttttgctt ctttagcaaa ttcgtccatg attttcgctg gtgcgactac atcttcgtta
    19201 ctgaatgcaa cagctgtagg tccagtgaat acttcgtcca tgccttcaag gccagctttt
    19261 ttagctgcac gtgaaagaat agaatttttg ataactttca tttctacgtt agcatcacga
    19321 agttgtttac gtaaacgtgt tacttcatca actgttaatc cgcggtagtc aacgactact
    19381 actgatgcag ctgctgtaaa tttttcgctt acttcatcaa cgattgctgc tttttttgcg
    19441 ataattgctt cactcatttt tgagtttcac ctcctgaatt ttgatggaag agttttttgc
    19501 actgaaaagg caagtcactt ttatagttac tttgctcaac cagtattatt catcacctat
    19561 tcacttcgtt catagtgatt ctactcgctt tgctcgaaag gaaactcctt tcgctttgct
    19621 caaccagtat tgttcatcat ctcttcactt cgttcatagt gattcacaat aaaaaactct
    19681 atgccaccgc agacatagag ggactattga atatattcaa taaacgtcct cggtaggaaa
    19741 ttaaggcttg atgccaccta ctgtcttcgg tacaagttaa ttattaacct caactagctt
    19801 agcatgctaa actagttgag tcaagaatta tttttcagtt ggatcaatca acattctaat
    19861 atttaattag aaagaagctt ggtcaacatg gataccaggt ccaaatgttg ttgtgatcgt
    19921 tacatttttc atgtattgac ctttagctgc tgatggttta gcttttagca atacatcatg
    19981 gattgctgca aagttttcaa ctaatttttc atcgtcaaat gaaacttttc cgattggtac
    20041 atggatattt ccagctttgt ccacacggta agttacttta ccagctttca cttcgtttac
    20101 tgcttttgtt acgtccattg taactgtacc agttttaggg tttggcatta agccttttgg
    20161 tcctaatact cgtcctagac gacctacagt agccatcata tcaggtgttg caacaactac
    20221 atcaaagtca aaccatccgt tttggatttt ttgaaccatg tcgtcgtcac ctacgaaatc
    20281 tgcaccagct gcttcagctt cttttgcttt gtctccttta gcaaaaacta aaacagtttg
    20341 tgttttacca gttccgtttg gtaatacaac agcaccacgg atttgttggt ctgctttttt
    20401 agggtctacg ttcaaacggt atgcaacttc aactgttgca tcaaattttg caatgtttgt
    20461 ttcttttgct aaagctactg cttcggcaac aggataaact ttacttgaat caactttttt
    20521 taatgcatct tgcatttttt tgctcttttt agccattttg gttcctcctt gattgtggtt
    20581 ataacggttg tacctcccac gtgttctata tctttcaata tagagccaac tgagaagctg
    20641 ctgtcttagg gtgtatagat tattctacag tgatccccat gcttcgtgca gttccttcaa
    20701 ccatgcgcat tgctgcttca acgttagctg cgtttaggtc ttccattttt aattcagcga
    20761 tttctcttac ttgatcgcta gaaactttcg caactttatt tttgtttggt tcgcctgaac
    20821 ctttttcgat tttagctgct tttttcaata gaacagcagc tggcggtgtt tttgtaacga
    20881 atgtgaatga acggtcttcg tatacagaaa taacaaccgg aatgatcaaa cctgcttgat
    20941 cagctgtacg agcgttgaat tcttttgtga atcccatgat attgataccc gcttgaccta
    21001 gtgcaggacc tactggtgga gctggagttg ctttacctgc aggaatttgc aatttaacga
    21061 ttttttctac tttctttgcc acgagacata cctccttaag tccgtgatgt ggttaattgg
    21121 agatgcatat ttctcctccc acgttttatc tgatctgaaa aaatccagac aaataatgtg
    21181 caaaacaatg cacactggta cagtatagca aatttatttg ttttttcaag atttttttct
    21241 gctattttta cattttgctt gaataaacgc taaatttatt ctaaaaaaac aacaattttt
    21301 cgttctcatt tcttctttta tgttacactt gcgctagcat ggttcaacag atgaaagcaa
    21361 acaataacac ggttttttag acccttgtac aaagattcct acataacaaa atgaggtgga
    21421 gaaaatgaaa gatttaaggt atagaagtgt ctttgatatt attggacctg tgatgattgg
    21481 tccaagtagc tctcacactg ccggagctgc tagaatcggg aaaatcatac gtagtatatt
    21541 tggcgaacag cctgattctg tagatattta tctatatgaa tcatttgcaa aaacctatcg
    21601 ggggcacgga acagatattg cattagttgg cggcctgtta gggatggaac ccgatgatga
    21661 acgtttagct gattctttga aattggcata tgaagccggt atggaagtct tgtttgttcc
    21721 taagagtgaa aaagccgaac atccaaactc tgtaaagatg cttctaaaaa aaggaacacg
    21781 aaagctttca gtcactggca tttcgatcgg cggtggcaat atccagattt ctgaattgaa
    21841 tggattcaaa atttctttaa gcatgggtac tcctaccttg atcgttgtcc accaagatgt
    21901 tccaggtatg attgctaaag tgactagtat actttctgag accaatacga atatcggcac
    21961 tatgacagtg acacgggaat caaaaggtga aaatgccatt atgataattg aagtagatga
    22021 tcctcaagta gaagaaacgg taaaaagact caaacagctg ccaaatatcg acagcgtaaa
    22081 ttatttcgat taaggagcta aaaaaatgtt tttatcgatt gaagaattag tagatcaagc
    22141 aaaagcttat ccaagtgtag cagaattgat gattgcagtt gaaatggaaa tgagtggccg
    22201 ttcccgtagt cacatcatcg agatcatgga aaaaaacctc tcagtaatgg aacaatcaat
    22261 caacgaggga aaagaaggtg ttacctccgt cacaggaatt accggtggtg atgcaaaaaa
    22321 attagatgat tatttaaatg aggcagactt tttaagtgga gaaacaatct taaaagctgt
    22381 acgcaatgct gtagctgtta atgaagtcaa tgcaaaaatg gggttgattt gtgctacacc
    22441 gactgccgga agcgcgggtg ttgttcctgg tgtgctgatg gctgtccgag atcggcttga
    22501 tttgacacat gaacagcaat tggaattctt atttaccgct ggcgctttcg ggttagtcat
    22561 cgcaaacaac gcttctatca gtggtgcaga agggggatgt caagcagaag ttggttctgc
    22621 aagtgcaatg gcagcagcag ctttggtttg tgcaaaaaat gggactcctc atcaagcagc
    22681 acaagcagta gccattacat tgaaaaatat gatgggactg atttgcgatc ccgttgcagg
    22741 cttagttgaa gtcccgtgtg tcaaacgtaa tgctttaggc tcttctcaag cttttatctc
    22801 tgctgatatg gcattagcgg gtatcgaaag tgtcattccg ccggatgaag tcgttgctgc
    22861 aatgtaccaa gtcggacgtc aaatgccgtc gattttcaaa gaaacagcag aaggtggctt
    22921 agctgttacg ccaactgcta aacgacttgc ggaagagatt ttaaataaga gggcgtgata
    22981 aagctcctta atttcgagta ttaagagatt agttatcttc ataacaaaaa acgaagagag
    23041 gactaattgc ccttctcttc gttttttatt aatccatcaa tttcgtattt tttacttcat
    23101 cgatcgtttt tgttcctgca agttgcatcg tgatcgacaa ttctttgttc aggtgttcaa
    23161 aaacagatgt tactccttct gccccaccta gattcaaccc ataaatcact ggtcggccaa
    23221 tcgcaactaa gtcagcacca cttgccagag ctttgaagac atgctctcct cgacggacac
    23281 cagagtcgaa aatcactggt acacgtttgt tgactacttc ggcaattttt ggcaatactt
    23341 caaaggacgc aggacctcca tctagttgac gtccaccatg attggatacc cagataccat
    23401 ctgcccctgc agagatagcg attactgcat cttctggcga ttgtattcct tttacgataa
    23461 ctggaagatg ggtgatttct ttgattgttt tgatgtcatc tggtgtcaag ccttgttttg
    23521 cagctgcata aatttctgcg attccttttc cttcgccatt accacttgca ctttgttcgc
    23581 tatacgcagc taagttaggc attggtaatg gaaattggaa ttgattgatg acgtcttctt
    23641 cacggtaacc accgagcgtt gaatctgccg ttaaaatgat agctttagcc cctgcttcta
    23701 ctgctttgtt caaaatgaac tcattgaatc tgtcgtcttt actcatatat aattggaaaa
    23761 actgcggtgc gcctggcgct gcatcggctg cgtctttgat tgtcgtattt gcataagtgc
    23821 tgatggaaaa gattgatcca gcagcagcta ctcctttagc tgtatcagct tccccttttt
    23881 catgtgccaa tccttgggcc gctgatggcg cttggataat cggtgtatct aattcaattc
    23941 cgaaaacaga tgtatgcaaa tcggctgagt cgattccgcg taatatccgt ggcatgattt
    24001 tctttgtatt aaaagaagtc gtattttctt tcatcgtcca ctcatcttca gaaccaccgc
    24061 gaatatagcc aaaagctcct gcttccatgc gctcttttac acgaccttct agttaggcaa
    24121 tgtttacaat atcgattgga tgttcttcat tacttgtatg ataagtcata gtagatcctt
    24181 ctttcatctt cttgattaca tataaatcat actaaactta caaaaagtaa gcaaacattt
    24241 actctcagat acttttatcg ctattctttc caaataaatc gacttacaag ctgatctact
    24301 tcttggtttt catgaagcat actatgagag gcattttcac ctgtcactac tttttcctct
    24361 atttcatttc cgcgttgctt caacaacgca tagaccgcta atgcactgtt taacggtact
    24421 gttccatcac tcaagtctgt ttcatctagt tgaccagcaa tcaagaaaaa tcgagtggtt
    24481 ataggaatat taccgatcat ttgctggtaa tcttgatagc ggttagaaac gacagcaggc
    24541 ccctttgcta actcatctgt cagagactgc gcactatcat cgacaaaatc attaaaaggc
    24601 gcgccaattg ctgttaatgt ctgaacttgt ggcaatgacg tatcctgtcc atacgttcct
    24661 aaatagcgta gtgcactaac tcctcccatg gaatgcccca ctatgttgac tttatctatg
    24721 ccgtagtttt ctttcagata caaaagacaa gttttgatcc attctgcttg attccactcg
    24781 ttatttttat tatcttcaaa caatacttgg accatcggat tgtctgcttt tccagacaaa
    24841 tctcctgttg cttgtatttg tccatctgtt cctactgtta aaaccaattc ttttactgct
    24901 tgctgatttt tttccaaacg ccgcaacatc cctttaaagg aattttctgt cccgctgtag
    24961 ccgtggaaaa aaagtgttgg aatagtggtt ttttctatca ctttactgga ttctgccgtt
    25021 ttttctttat cagaaacttc agtagtattt atcttcgtcg tctggcaacc acttaacgca
    25081 aacaaactga taaggactaa agctgctgca gttgcacgtt tgcataaatt tttgattggt
    25141 tctgtcatac acctcttcct ttccatatag aaaaaagaaa gacttgaaag gccaatcata
    25201 tgatcgacat ttcaagtctc tttattaatc gattttatct acttgatcga aatcaagttc
    25261 tgtactggtt tcacgtccaa acatatcgat gttgactttc aatttttctt tttctccgtc
    25321 aacttctgtt acttgtcctt ctaatccagc aaatgcgcct tcgatgattt tcacgacttc
    25381 gccaacttct acttctagat cattttgtcg caggctcata ccgatagaac gcaagatatg
    25441 attgacttct tctggaagta atggtgctgg tttgcttccg gctccgtgtg aaccaacgaa
    25501 tcctgtcaca ccaggtgtgt tgcggacaac ataccatgaa gcgtctgtca tgatcatttc
    25561 aacaagtacg tatcctggga atgttttatg aacgatttct ttttctttcc catttttgac
    25621 ttcagtttct ttttcttcag gaacaacgac acggaaaata aagtcggcca ttttcatact
    25681 ttgcgcacga gattcgatat ttgcttttac tttgttttca tatcctgaat atgtatgcaa
    25741 gacataccag tttttttcaa aagattccat tgaaggctcc tcatttcatt gtaaaataaa
    25801 aaaacctcag tacacccgaa gtttttcttt cgcttcatta tagcactaaa agtacgttgt
    25861 ggctagcgag ttattttaaa atccagccaa ataagctttg gatggctgta tccattacgt
    25921 aaaacatcaa tccgaaaata atagaagttt cgatgactac taacgtatct ttacggagtt
    25981 gttttctgct tggccaagtt acttgtttga tttcatcttt tacgcttttt agaaatttca
    26041 tgagacaacc tcccttgcta ctgtttattt cgtttcttta tgtaatgtat attgattaca
    26101 atatttacaa aacttattga tttccaaacg ttcgccgctt tttccttcac tgactgattt
    26161 tgtgtagttg cgagacccac aaacggaaca agcgagtgct gcttttttct ttgccattta
    26221 cttctgcccc cttgctgaga atcagactta tacttcataa cagtatcacg taacttttta
    26281 cttgtcaatt tgtgaaataa actatctatt gttttggata aaattatgat atgattaatt
    26341 tgaaattaag ctaaaatgag acaattggag gtatagcgat gctattaaaa acacttgtat
    26401 ataagaaaaa agatctgaca accgttacag agactagtac attagaagaa gcattaaaaa
    26461 ttttagaaac ttccggatac cgttgtgtgc cgatcttaga ttcttcaggt aatattttcc
    26521 gtggaaacat ttacaaaatg catatctatc gtcataaagc aaatggcggt gatatgacgc
    26581 ttcctgtcac tcatcttttg aaaaacgcta caaagttcat ttacctggat acttctttct
    26641 tcaaagtatt ctttacgatc aaagagcttc cttatattgc tgtccttgac agtgaaaatc
    26701 atttttatgg tattttgaca catagtacgt tgcttaatat gctctctcaa tcttggagcg
    26761 tagaacaagg aagctatgta ctgacaattg cttccacagg aaagcaagga gatttggctg
    26821 ctattgcaaa aatcatttca aaacacagca gcattgccag ctgtattact cttgatatcg
    26881 aaaaagacga atacattcga cgtacattga tcacgctacc ggctaacact gaagcgaaag
    26941 tatgtgaaac gatcgtcaac cacttagaga aaaagaatta taaagtggta gaaattgaaa
    27001 atttgcaaaa ccaagaagca taatcaaaaa gcgaaaaaag aaaacagcta atctgctttc
    27061 ttttttcgct ttttataatt agacacgctt ttcaaaatca agcgcgcgat ctaaataata
    27121 catcaatggt gctgaaacag ccatgacgat aaactcactt aatgcaagcg tgccataggt
    27181 tgtccaaaat gcttggccac ctgttggaac taacatataa gcaatcagtc ccatgctgat
    27241 ggtaaagaag atcgtgttca atactaaacg catctttaca tttttcactt tgttttgcaa
    27301 taaagctgtc aggcctaaag aaagcaatgt ttggccgcca ccaaaaacca catccatgat
    27361 accaaaacca aagaaggcat tatagacgat gaccccgcct aaaactcccc aaagcaattt
    27421 acggttaaag acaactaaat gattcaaact ttctgaaata cgaaactgga tcggccctga
    27481 ggatacaggc gcaactaaaa tagttaacgc tagataaaga gccatgatga tgccatttac
    27541 aacgattacg gataaccgtt gtctgctttt cgttttttgc attttctctc ctcttttctc
    27601 ctagtttttt taacgcggga tggttgatga accgcggtta tgtgaaacaa acaataatta
    27661 taacagactt ttcatttatt tcaatccgtc ttatctgctt ttaaaaaagc aaaagcttaa
    27721 acaatgtaaa cgtttgctaa aaatcgtggg atatggtata aagtaagtgt aaggggtcat
    27781 aagaattccc agtaaagcag acgaagtagg atctgcaaga acagaattgt tgtgaaactt
    27841 aaataaataa aaaaggatgt gggaatttga gcaacctctt actagagaac ggtctagcaa
    27901 gcagctgaag atctaagtgg acatgccgaa ccttggaact tctccttacg taagtaatgg
    27961 atcatgcgct tgctaggctt ttttgtgttt ttcggtcttc ggaccttttt cattttccta
    28021 gacttctgct ataataacca agaattaatt ttgtaacaga tttttttgaa aaaacaagtt
    28081 tctttcattc aaaaaataga aaaagcttgc taattattgg ttaaccagca aacaggagta
    28141 ttaaaatatc ttgttttatt gaatagcagg aggaaatcat gaaattccat tatcatatat
    28201 tgatcaatcc atcggcaggc agcggaaatg gctataaagt agcagaaaga attttaccgg
    28261 tattaaaaaa taagcatatt gactatacac ttcattatag tgaatacaaa ggacacgatg
    28321 ctgagattgc tgaaactttg gcaaaagaaa cattgattcc ttggatagaa gaagaacaga
    28381 aaacaatcga tactttcccc cttttgatca tcgtaggagg tgacggcacc ttacatcagg
    28441 tacttgatac tttctatcag atggaagtag aattcccagt tgcttatatt ccagcaggtt
    28501 ctggagatga ttttgctcga ggggcaggcc ttccaaaaaa tcccaaaaaa ggacttcagc
    28561 tgattttagc agctcaatca ccagaaaaag tccacataat ggcttatgaa gaaaaaataa
    28621 gtgaaaaaaa aggaatagct gttaataatt tcggtatagg attagatgca gctatcgtcc
    28681 atgcgacaaa tcattcttcc acaaaaaagc gattgaacaa atacaactta ggatcatttt
    28741 cgtatttatt ctctattctc cgtgctttat tcactcaaaa aggatttcca attctggtag
    28801 atgcaggagg aaaacaatta agtttttcaa acgcattttt atgtacagca acgaagcatc
    28861 ctttttttgg cggaggaatc gcgattgttc caactgctga tgcgtctaaa ccagtcattg
    28921 attttgtcgt ggtcgaacga atcaatttct ttaaaatctt gtggctcatt cttttactta
    28981 tacagaaaaa acaattgaaa tcgaaatatt tccatcacta cacaacaaac agattacgta
    29041 tcgtttcaac aacaccgcaa catggtcaag aagatggaga agaaatggaa aaacgacctt
    29101 ttgatatcac aataaccaca actaaacagt tgttttggtg ttaataaaag aggttttgtc
    29161 agaagctaca tctggaacac cgtttattcg gaaaaaagga ctgtgacacg acttttgtca
    29221 cagtcctttt tttactatgc agttaattct ttgcccaatt gggttgccat cgatagtttc
    29281 tttctttacc caaaataaat tggatcattg aaaacaccgc agcaaaagca actaagacac
    29341 aaccattgaa ccaaaaagat tctgcaaatg tttgattgta actgagtgtt tcactgatca
    29401 agttattatt ttgaacaaac caatatgttg gtgtgaatgc cgctattttg ctaaccattt
    29461 ctggcaagta ttctgctggt acaaacaccc caccgataaa gcaactgcct gtgataaaaa
    29521 tatctttgat cccaccgctg gtttctgagt tttttaccaa tgaagtgata caagccgaaa
    29581 agctaatgat cggcaataaa aacaagaaag tatttaaaat atgataccat accgttatat
    29641 cccacgaggt gtggatcact aacagaacca ttagaagtaa gaagccactg aacaacagca
    29701 gcgagtaact aatcaaacta aaggttactt tgcgattcaa gtttcgtttg gtaatgggcg
    29761 cactctggtt tcgtttactg atctctttgc gattaaatgc tagattgatg acgctgaaac
    29821 cgctaaaaat ggatagaaac aacccataag acaagaaatt aaaggttgtg cccgtcaaca
    29881 gctttttcat ccgctgacta tatcccgcac ttagctcaac ttctccagta accgagagtg
    29941 ccatttgtgt ctgttccata gctgcatcga ttgttttatc ttcttcttga aacagaatca
    30001 gtgtttgcaa aaaggtgttc acttgctgtg taacaactgt tttagaaaag gcatctgggc
    30061 gactctgtat cttgatcgtc ggcatctttc ctacttcgac ttgttcggta aagtctgcag
    30121 gcagatgtaa gataatgttt acacgattaa aataaagagc atcatcgatt tctttttgtg
    30181 aggttccatc caatgtaacg actgtttcct tgtcattcaa atatttacga aggccatcga
    30241 ctacaggcga ctcttcttca ctaatcaagg cgattttcgc atcttgcaga gaggtatttt
    30301 caacatcaat attttgcgca aaaaagaatg tggcaacaac agtgataatt acacctatca
    30361 gtaaattcga tttatttgct ttaagcaatg tccaaatcgt tttataaagc gtcataacgc
    30421 acccctcttt ccatccaagc atttcctaac gcaaaaagga aaataaatcc gatcaaccat
    30481 agaagattgg tataaaaact agccaaatca gtgtagaaat aaagttt
//
LOCUS       DAZUKR010000020        30123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000020 DAZUKR010000000
VERSION     DAZUKR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.020"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..612)
                     /locus_tag="ACG89C_000823"
     CDS             complement(34..612)
                     /locus_tag="ACG89C_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4899398.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(689..1219)
                     /locus_tag="ACG89C_000824"
     CDS             complement(689..1219)
                     /locus_tag="ACG89C_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS4899399.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1223..2083)
                     /gene="rlmB"
                     /locus_tag="ACG89C_000825"
     CDS             complement(1223..2083)
                     /gene="rlmB"
                     /locus_tag="ACG89C_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4899400.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2076..2471)
                     /locus_tag="ACG89C_000826"
     CDS             complement(2076..2471)
                     /locus_tag="ACG89C_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS4899401.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2468..3880)
                     /gene="cysS"
                     /locus_tag="ACG89C_000827"
     CDS             complement(2468..3880)
                     /gene="cysS"
                     /locus_tag="ACG89C_000827"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4899402.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3877..4413)
                     /gene="epsC"
                     /locus_tag="ACG89C_000828"
     CDS             complement(3877..4413)
                     /gene="epsC"
                     /locus_tag="ACG89C_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS4899403.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4499..4738)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4735..6195)
                     /gene="gltX"
                     /locus_tag="ACG89C_000829"
     CDS             complement(4735..6195)
                     /gene="gltX"
                     /locus_tag="ACG89C_000829"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4899404.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6365..7522)
                     /locus_tag="ACG89C_000830"
     CDS             complement(6365..7522)
                     /locus_tag="ACG89C_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS4899405.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7556..8926)
                     /gene="radA"
                     /locus_tag="ACG89C_000831"
     CDS             complement(7556..8926)
                     /gene="radA"
                     /locus_tag="ACG89C_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4899406.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8989..9516)
                     /locus_tag="ACG89C_000832"
     CDS             complement(8989..9516)
                     /locus_tag="ACG89C_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4899407.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9746..10552
                     /gene="proB"
                     /locus_tag="ACG89C_000833"
     CDS             9746..10552
                     /gene="proB"
                     /locus_tag="ACG89C_000833"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4899408.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10549..11796
                     /locus_tag="ACG89C_000834"
     CDS             10549..11796
                     /locus_tag="ACG89C_000834"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4899409.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11872..12216
                     /locus_tag="ACG89C_000835"
     CDS             11872..12216
                     /locus_tag="ACG89C_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899410.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12333..12659)
                     /locus_tag="ACG89C_000836"
     CDS             complement(12333..12659)
                     /locus_tag="ACG89C_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899411.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13274..14476)
                     /locus_tag="ACG89C_000837"
     CDS             complement(13274..14476)
                     /locus_tag="ACG89C_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4899412.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14593..15108
                     /locus_tag="ACG89C_000838"
     CDS             14593..15108
                     /locus_tag="ACG89C_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4899413.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15307..15753)
                     /locus_tag="ACG89C_000839"
     CDS             complement(15307..15753)
                     /locus_tag="ACG89C_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4899414.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15800..17278)
                     /gene="msr(C)"
                     /locus_tag="ACG89C_000840"
     CDS             complement(15800..17278)
                     /gene="msr(C)"
                     /locus_tag="ACG89C_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4899415.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17632..18261)
                     /locus_tag="ACG89C_000841"
     CDS             complement(17632..18261)
                     /locus_tag="ACG89C_000841"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4899416.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18245..18709)
                     /locus_tag="ACG89C_000842"
     CDS             complement(18245..18709)
                     /locus_tag="ACG89C_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4899417.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19196..20464)
                     /locus_tag="ACG89C_000843"
     CDS             complement(19196..20464)
                     /locus_tag="ACG89C_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4899418.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20695..21432)
                     /locus_tag="ACG89C_000844"
     CDS             complement(20695..21432)
                     /locus_tag="ACG89C_000844"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4899419.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21499..22068)
                     /locus_tag="ACG89C_000845"
     CDS             complement(21499..22068)
                     /locus_tag="ACG89C_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4899420.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22196..23068
                     /locus_tag="ACG89C_000846"
     CDS             22196..23068
                     /locus_tag="ACG89C_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4899421.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23410..24282
                     /locus_tag="ACG89C_000847"
     CDS             23410..24282
                     /locus_tag="ACG89C_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4899422.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24279..26237
                     /gene="nagE"
                     /locus_tag="ACG89C_000848"
     CDS             24279..26237
                     /gene="nagE"
                     /locus_tag="ACG89C_000848"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4899423.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26569..27420)
                     /locus_tag="ACG89C_000849"
     CDS             complement(26569..27420)
                     /locus_tag="ACG89C_000849"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4899424.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27543..28010
                     /locus_tag="ACG89C_000850"
     CDS             27543..28010
                     /locus_tag="ACG89C_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS4899425.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28273..28755)
                     /locus_tag="ACG89C_000851"
     CDS             complement(28273..28755)
                     /locus_tag="ACG89C_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4899426.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28855..29718)
                     /locus_tag="ACG89C_000852"
     CDS             complement(28855..29718)
                     /locus_tag="ACG89C_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4899427.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8598 a         6373 c         5455 g         9697 t
ORIGIN      
        1 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
       61 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
      121 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
      181 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
      241 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
      301 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
      361 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
      421 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
      481 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
      541 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
      601 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
      661 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
      721 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
      781 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
      841 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
      901 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
      961 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
     1021 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
     1081 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
     1141 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
     1201 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
     1261 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
     1321 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
     1381 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
     1441 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
     1501 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
     1561 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
     1621 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
     1681 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
     1741 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
     1801 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
     1861 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
     1921 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
     1981 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
     2041 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
     2101 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
     2161 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
     2221 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
     2281 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
     2341 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
     2401 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
     2461 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
     2521 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
     2581 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
     2641 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
     2701 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
     2761 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
     2821 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
     2881 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
     2941 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
     3001 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
     3061 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
     3121 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
     3181 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
     3241 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
     3301 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
     3361 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
     3421 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
     3481 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
     3541 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
     3601 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
     3661 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
     3721 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
     3781 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
     3841 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
     3901 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
     3961 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
     4021 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
     4081 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
     4141 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
     4201 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
     4261 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
     4321 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
     4381 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
     4441 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
     4501 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
     4561 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
     4621 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
     4681 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
     4741 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
     4801 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
     4861 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
     4921 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
     4981 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
     5041 agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc taacgatctt
     5101 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
     5161 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
     5221 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
     5281 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
     5341 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
     5401 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
     5461 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
     5521 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
     5581 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
     5641 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
     5701 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
     5761 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
     5821 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
     5881 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
     5941 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
     6001 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
     6061 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
     6121 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
     6181 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
     6241 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
     6301 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
     6361 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
     6421 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
     6481 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
     6541 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
     6601 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
     6661 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
     6721 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
     6781 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
     6841 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
     6901 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
     6961 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
     7021 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
     7081 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
     7141 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
     7201 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
     7261 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
     7321 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
     7381 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
     7441 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
     7501 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
     7561 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
     7621 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
     7681 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
     7741 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
     7801 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
     7861 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
     7921 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
     7981 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
     8041 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
     8101 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
     8161 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
     8221 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
     8281 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
     8341 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
     8401 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
     8461 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
     8521 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
     8581 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
     8641 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
     8701 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
     8761 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
     8821 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
     8881 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
     8941 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
     9001 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
     9061 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
     9121 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
     9181 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
     9241 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
     9301 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
     9361 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
     9421 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
     9481 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
     9541 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
     9601 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
     9661 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
     9721 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
     9781 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
     9841 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
     9901 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
     9961 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    10021 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    10081 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    10141 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    10201 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    10261 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    10321 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    10381 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    10441 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    10501 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    10561 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    10621 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    10681 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    10741 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    10801 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    10861 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    10921 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    10981 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    11041 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    11101 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    11161 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    11221 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    11281 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    11341 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    11401 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    11461 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    11521 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    11581 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    11641 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    11701 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    11761 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    11821 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    11881 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    11941 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa
    12001 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    12061 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    12121 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    12181 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    12241 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    12301 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    12361 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    12421 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    12481 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    12541 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    12601 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    12661 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    12721 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    12781 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    12841 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    12901 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    12961 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    13021 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    13081 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    13141 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    13201 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    13261 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    13321 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    13381 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    13441 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    13501 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    13561 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    13621 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    13681 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    13741 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    13801 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    13861 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    13921 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    13981 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    14041 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    14101 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    14161 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    14221 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    14281 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    14341 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    14401 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    14461 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    14521 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    14581 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    14641 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    14701 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    14761 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    14821 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    14881 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    14941 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    15001 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    15061 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    15121 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    15181 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    15241 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    15301 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    15361 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    15421 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    15481 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    15541 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    15601 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    15661 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    15721 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    15781 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    15841 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    15901 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    15961 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    16021 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    16081 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    16141 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    16201 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    16261 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    16321 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    16381 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    16441 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    16501 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    16561 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    16621 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    16681 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    16741 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    16801 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    16861 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    16921 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    16981 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    17041 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    17101 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    17161 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    17221 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    17281 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    17341 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    17401 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    17461 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    17521 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    17581 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    17641 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    17701 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    17761 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    17821 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    17881 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    17941 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    18001 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    18061 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    18121 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    18181 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    18241 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    18301 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    18361 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    18421 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    18481 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    18541 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    18601 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    18661 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    18721 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    18781 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    18841 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    18901 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    18961 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    19021 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    19081 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    19141 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    19201 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    19261 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    19321 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    19381 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    19441 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    19501 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    19561 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    19621 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    19681 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    19741 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    19801 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    19861 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    19921 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    19981 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    20041 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    20101 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    20161 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    20221 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    20281 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    20341 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    20401 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    20461 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    20521 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    20581 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    20641 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    20701 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
    20761 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    20821 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    20881 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    20941 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    21001 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    21061 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    21121 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    21181 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    21241 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    21301 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    21361 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    21421 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    21481 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    21541 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    21601 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    21661 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    21721 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    21781 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    21841 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    21901 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    21961 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
    22021 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    22081 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
    22141 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    22201 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    22261 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    22321 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
    22381 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    22441 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    22501 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    22561 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    22621 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    22681 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
    22741 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    22801 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    22861 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    22921 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
    22981 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    23041 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    23101 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
    23161 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    23221 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    23281 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    23341 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    23401 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    23461 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    23521 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    23581 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    23641 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    23701 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    23761 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    23821 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    23881 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    23941 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    24001 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    24061 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    24121 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    24181 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    24241 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    24301 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    24361 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    24421 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    24481 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    24541 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    24601 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    24661 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    24721 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    24781 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    24841 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    24901 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    24961 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    25021 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    25081 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
    25141 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    25201 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    25261 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    25321 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    25381 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    25441 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    25501 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    25561 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    25621 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    25681 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
    25741 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
    25801 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    25861 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    25921 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    25981 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    26041 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    26101 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    26161 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    26221 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    26281 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    26341 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
    26401 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
    26461 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
    26521 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    26581 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    26641 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    26701 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    26761 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
    26821 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    26881 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    26941 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    27001 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    27061 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    27121 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    27181 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    27241 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    27301 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    27361 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    27421 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    27481 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    27541 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    27601 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    27661 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    27721 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    27781 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    27841 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    27901 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    27961 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    28021 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    28081 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    28141 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    28201 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    28261 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    28321 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    28381 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    28441 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
    28501 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    28561 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    28621 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    28681 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    28741 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    28801 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    28861 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    28921 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    28981 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    29041 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    29101 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    29161 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    29221 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    29281 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    29341 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    29401 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    29461 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    29521 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    29581 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    29641 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    29701 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    29761 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    29821 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    29881 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    29941 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    30001 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    30061 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    30121 aaa
//
LOCUS       DAZUKR010000021        29030 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000021 DAZUKR010000000
VERSION     DAZUKR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.021"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..577
                     /locus_tag="ACG89C_000853"
     CDS             257..577
                     /locus_tag="ACG89C_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS4899428.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            731..1636
                     /locus_tag="ACG89C_000854"
     CDS             731..1636
                     /locus_tag="ACG89C_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899429.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1633..2406
                     /locus_tag="ACG89C_000855"
     CDS             1633..2406
                     /locus_tag="ACG89C_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4899430.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2587..4011)
                     /locus_tag="ACG89C_000856"
     CDS             complement(2587..4011)
                     /locus_tag="ACG89C_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4899431.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4222..4668
                     /gene="nrdI"
                     /locus_tag="ACG89C_000857"
     CDS             4222..4668
                     /gene="nrdI"
                     /locus_tag="ACG89C_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4899432.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4733..6715)
                     /locus_tag="ACG89C_000858"
     CDS             complement(4733..6715)
                     /locus_tag="ACG89C_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4899433.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6729..7625)
                     /gene="murQ"
                     /locus_tag="ACG89C_000859"
     CDS             complement(6729..7625)
                     /gene="murQ"
                     /locus_tag="ACG89C_000859"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4899434.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7628..8689)
                     /locus_tag="ACG89C_000860"
     CDS             complement(7628..8689)
                     /locus_tag="ACG89C_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4899435.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8900..9427)
                     /locus_tag="ACG89C_000861"
     CDS             complement(8900..9427)
                     /locus_tag="ACG89C_000861"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4899436.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9494..10996)
                     /gene="eat(A)"
                     /locus_tag="ACG89C_000862"
     CDS             complement(9494..10996)
                     /gene="eat(A)"
                     /locus_tag="ACG89C_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4899437.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11254..12375)
                     /locus_tag="ACG89C_000863"
     CDS             complement(11254..12375)
                     /locus_tag="ACG89C_000863"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4899438.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12501..13442
                     /locus_tag="ACG89C_000864"
     CDS             12501..13442
                     /locus_tag="ACG89C_000864"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4899439.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13592..14488)
                     /locus_tag="ACG89C_000865"
     CDS             complement(13592..14488)
                     /locus_tag="ACG89C_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4899440.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14757..16097
                     /locus_tag="ACG89C_000866"
     CDS             14757..16097
                     /locus_tag="ACG89C_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4899441.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16315..16758
                     /locus_tag="ACG89C_000867"
     CDS             16315..16758
                     /locus_tag="ACG89C_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4899442.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17262..18680)
                     /gene="nhaC"
                     /locus_tag="ACG89C_000868"
     CDS             complement(17262..18680)
                     /gene="nhaC"
                     /locus_tag="ACG89C_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4899443.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19083..20306)
                     /locus_tag="ACG89C_000869"
     CDS             complement(19083..20306)
                     /locus_tag="ACG89C_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4899444.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20337..21176)
                     /locus_tag="ACG89C_000870"
     CDS             complement(20337..21176)
                     /locus_tag="ACG89C_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4899445.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21193..21873)
                     /locus_tag="ACG89C_000871"
     CDS             complement(21193..21873)
                     /locus_tag="ACG89C_000871"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4899446.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21875..22948)
                     /locus_tag="ACG89C_000872"
     CDS             complement(21875..22948)
                     /locus_tag="ACG89C_000872"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4899447.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23004..23242)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23285..23518)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23595..24266)
                     /locus_tag="ACG89C_000873"
     CDS             complement(23595..24266)
                     /locus_tag="ACG89C_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4899448.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24353..27001)
                     /gene="mgtA"
                     /locus_tag="ACG89C_000874"
     CDS             complement(24353..27001)
                     /gene="mgtA"
                     /locus_tag="ACG89C_000874"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4899449.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27343..27517)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27690..28127
                     /gene="ndk"
                     /locus_tag="ACG89C_000875"
     CDS             27690..28127
                     /gene="ndk"
                     /locus_tag="ACG89C_000875"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4899450.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28149..29015)
                     /locus_tag="ACG89C_000876"
     CDS             complement(28149..29015)
                     /locus_tag="ACG89C_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4899451.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
BASE COUNT         8375 a         6104 c         5156 g         9395 t
ORIGIN      
        1 taataaataa caacttcttt ctatgaaatt gcaaagcatt catcttcttt tccgctaacg
       61 atatatccgt cagtgcttgg tacactttac taaaaagtat gagattgccg atatctggat
      121 aggtgtgcta tactacagaa aaagatatac aatctgccac cgggtaggat aaagaggatg
      181 atcattccat taggtctttg aagggatgat tttcctcttt ttgtgtattt ttttatagtt
      241 aaggaggaat gtaagtatgg catggatgga attaatcata gcaggaattt tggaagtatt
      301 ctggtcaacg atgatgaaat ggagtgatgg tttttctaaa atcaattatt ctttttacac
      361 agtgattggc atgattgcta gtttttactt tttgtcaaag gcaatcaaaa gtttgccgat
      421 gagtctctct tatcctatct ggacagggat tggcgcagtt ggttcggtca taattggtgt
      481 ggtcttgttt catgataagc tgaatctttc cacttggttc tttgtgggga tgctactcat
      541 cagtatcatt ggcatcaaga ttactagcgg ccactagaca cttggaataa gtactgatca
      601 gaaaaaataa gcctttttat ttaatattca atttttttat gaaaaaataa gaaataagtc
      661 caatgaccta gcgacttctc cactcatcag gtgtatgctt gtactatcta tagaaaagga
      721 gtgaatattt ttgaaaagtc tttcggtcca agaattatcc aagaactttg gtcacaaaca
      781 agccctcagc gatgtcagtt ttagcttatc ccctggcaaa atcgttggat tagtaggtcc
      841 taatggcgct ggaaaaacaa cgatcatgaa agctatttta ggactgatcc attactcaga
      901 tggaacaatc tgtattggtg aaaatgagat ttctccctct agtcataaag ggttggaaca
      961 agtaggagca ttgatcgaat acccgggtat ctatccattc ttaacaggct atgatcatct
     1021 gaagttattc tctgaaacca acgatgtatc tgctatcgac actatcgtga atcagctcaa
     1081 gatggaaaag tacattcaca aaaaagcgaa atcctactcg ttgggaatga aacaaaaatt
     1141 gggaattgct cttgccttac tgaaccatcc gtctatcgtt attttagatg agccgatgaa
     1201 cggtttggat ccacaagcaa caagagatgt gcgaaaaatc attaccgact tggctactca
     1261 gggcacctct ttcttgatct ccagtcactt actcagcgag cttgaaaaaa tggtcgatga
     1321 gctgatcctg atcaatcaag gaaagatcat caaacaatgt acaatggctg aattgaacca
     1381 aacagcgcaa gatttcatcg ttatctctac aacaaatgat ctagctgctc gaacgatctt
     1441 gacagaagct ggatatactt tggaagatcc tgataaaatc aagcttttaa aagaaaacga
     1501 agaattatta ggaaccgtga tccagctatt gtctgcacaa aatatccatg tgacagacgt
     1561 ccagcatctg acgaatgatt tggaatcttc tctattggaa attctggatt cagtaaaggg
     1621 ggaagaagaa aaatgacatt gcttgtaaaa caagaattat ttaaattgat caagaaaaag
     1681 tcaactgctg ttttatctgt tctattagtt gttttgctca taggaacagc acttctagcc
     1741 aaaaaataca cgacgatcat tgatccagtt gaaatgactg ctcagctttt tagtgccaca
     1801 tcttggattg tctttatcat gattgcagct gcaagcacga tcatttcgat ggaagcacaa
     1861 tatggtacat tgaaaaatct cctttatcga aagtattctc gaggagaaat attagtaagc
     1921 aaatggatca cattagttat ttattctgtt tatttatacc ttttagctat tattgtcact
     1981 gtgttgatga aactgattct tttcccttcg atttccttca cggagaaggt ttctactggt
     2041 caaacactga tccaaagctt gttcacttat actttgggta gttatatcgg tttatggctg
     2101 attttaagtt tagtgttgat gattgcttgc tttatcaata gttccggcgc atccatctca
     2161 gcaggaatcg tcttttattt tgcttcgtca atcatatctg gtatccttat tgcactcatc
     2221 caaaaatggg agtggatcaa atggaatccg atcagtatgt tgaatctgca aaaccaaatt
     2281 ggaaacgaag agatcatgaa gcaattgact catctttcta ctaatcaaat gctattcggt
     2341 aatttagctt acatcgtttt gttcttagca ttaggatatc tcgtatttaa gcgaaaaaat
     2401 gtatgatgac tcatcaaaaa agaggctgtg aaagagacgt tcagccttat tgccgattgg
     2461 ctgtctctta aacaccgttt attctgaaaa aggagctgtg acaacatttg tgtcacagct
     2521 cctttcctag tatgtttttg aataaacgac aggctcacaa cctctcttta tacttctttt
     2581 atacttctat acgtgaagca tcccagtggc aaataaaacc attgcaatca ctccgaccat
     2641 cgtcacacct ataagtaaaa tcttatctaa gtggatatgt tgtacaggct cgttttgctc
     2701 cttacgtgct ttgaggtaaa aataaatgcc tggcaagaaa agaacaagaa caagcattac
     2761 ttcattaaag gctacgacta gcatagccca tacttgaaag gcacttgcta aaagccctac
     2821 taaaaattgg acccattctt tccgctgata agaaagtttc atttggtaca atcctaagaa
     2881 aagccacgtg aagaaaatag ctgctgtacc aagtgtgtaa gcaaagttat aagcactgtc
     2941 agtgaataat aatgaaagta aaaagacatt cgtacataac gtcgtcaata cgatagcata
     3001 agttggtgcc ccatttttgt tgagttttcc ccatgcttta ggcaaaagtc cgtcttcagc
     3061 catgtttccc attgtttcaa tcggtaagat cgtccatgag agccaaacac caactaaaga
     3121 aatgatcaaa ccgccgttga tcacgactgc tccccattta ccaaccatca tttcaaacac
     3181 atagcccata gcaggctgcg ccttgatcgt ttgcagctga ttcatcgtca ttgtcccata
     3241 tggaagcatg gaaaccaaga cgtaaataat aatcaagccg aatatcccta gaatcgttgc
     3301 tttcccaaca tctgattgtt tttttgctcg tgaacctacg acagaggctc cttcaatacc
     3361 aacaaatacc caaagcatca ccatgatgca actcaatact tgactggaaa acgggatcgg
     3421 ctgtccattc gcattcaata taatgtcgct agaaaagtta tcaatgaatt gttggatcga
     3481 aaacgtgctg atagctacta caataaataa aaataatggc actaatttac agaatgtgac
     3541 gatagcattg acgaaactag cattttcgac tccccgattc actagccatg catatcccca
     3601 taaaaagact gtactcatca cgatcgatgc cagattttgc ccaccttcaa atactgggaa
     3661 gaaatatcct aatgcgctca tcaacaatgt tgcaaaagct acgttgccta accagcatgt
     3721 gagccagtaa ccccaaccgc tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta
     3781 actaaagatc ccgcctttta aatctggacg ttttttattg atattattga tcgacaagga
     3841 gagcatcaac attgctgttc ctacgagtac ccaactgaaa atagctgggc cgccagccga
     3901 tccggagatg tccgtcataa taccaaatac gccacctcca atcgctgaac caattactaa
     3961 ggcagcaagt ggaattaaac caattccttt tttctgttgc tctgtatcca tgtaagcatc
     4021 ctccaaaata aaaccgcttt tatcaaaaag tataacaaac cttttttatt tttacttaat
     4081 taataaaaac tttaatagaa atataattct accggctttt ttctcattat acaagcaaag
     4141 cgcctagtcc ctttccaaat tacaccacca aaaaacttaa aaaagatgct atactaaaag
     4201 gaaagaattg gaggatgatc catgaacatt ttatatatat cgatttcagg aaatacgcgt
     4261 gcctttgcca aacatctagc agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat
     4321 cctgaagtta ctttaaaaga aatccatgaa aatagtgatt ttacgaaaga aacagaacca
     4381 ttttttactt tcgttcctac ttatttagac ggcggaaatg gtttagataa cggtgataca
     4441 gaaatcttga ccgaaacgat gcgtgaatat ctggaacatg aagacaatca taaactttgt
     4501 cttggtgtag tcggtagcgg aaataaaaac ttcaataatc agtattgtct aacagccaaa
     4561 caatatgcac aaacatttgg ctttcctttc ttggcagatt atgaattacg gggaacacca
     4621 tctgatgtga cacgaattta tcagatttta gcagaaaaca ataaataata aaaaagaaga
     4681 agctgaaaaa tagatttcag tttcttcttt cgtttctatt gtttctatca tattaagctt
     4741 tgacatccat cacaggtgct tttgcttttg ctttacctgt tttttccaag acaaagtttt
     4801 ttacacgatc catattggtt accaagacga tcatggatgt ttctttgcct gctgctttga
     4861 tcgcttctaa gtcagcttct gctacaagcg tatcaggtgt cacttgctgc ccttctgtta
     4921 ctttcacatc aaatggcgta ccgcctaagt caactgtgtt gattcccata tgaagcaaga
     4981 tttctaaacc atttggcgtt gtgatcccaa tagcgtgttt cgttgggaag acagagctga
     5041 ctaccccagt aactggagaa tagatatgtc cgttttctgg ttctacaaag tagccttcac
     5101 ccatcatctt ttgtgcaaaa actggatcac ttgctttatc taaagaaaca actgttccgt
     5161 cagcaactga tgagaggata gtcacacttt cagctgaagt ttgaggtact actttttcag
     5221 gcacttcatt tactgtggct gtttcagtcg ctaattcttc tttttctaaa cggtctttag
     5281 gaataccgaa gaagaatgta gcaacgaagc caccagcata agctgccaat aatccaagga
     5341 tgtagcctaa ccatttaccg tcagagatca atgggatcaa agcggctcct gaaggtccaa
     5401 tagcgatagc cccgacattt ccgatcattc cgatcactgc cccgccgata cctccaccga
     5461 tacaagcagt aataaatggg cgtcctagag gtaatgttac tccgtaaatc aatggttcgc
     5521 cgattcctag gatcccgact ggtaaagctc ctttgatcat ttctgttaat tctttatctt
     5581 ttttgcagcg aatccataat gccaatgctg cacccacttg tcctgcacca gccatcgcaa
     5641 gaattggaag caatagcgta cgaccagttt catcaatcat ttgcacatgg attggagtca
     5701 aaatttgatg cagtccgaac ataaccatcg gcaagaataa tgtgcctaga acgaagccgg
     5761 caaatgctcc accaacgcca agtacccagt tgatcccacc gaccatgcca tcagaaatga
     5821 aacctgctaa aggcatgatg aagaaaattt caatcagacc gatggcaatc aatgaaagag
     5881 ttggtgtaac gatgatatct accgcatcag gtactacttt atgcagtttt ttctcaacga
     5941 ttgacaatag ccaaacagcg aagatgacac ctaggatccc accttgtcca gctgcaagag
     6001 cttcaccagt aaataagttt ttcagtggtg cttccggatt catccctgtc agcatcacaa
     6061 cggccccgat aactccacca agagttggtg ttgcaccaaa aacttgtgca gcattgatac
     6121 ctgtatagat aactaggtag ctaaacatcc cgtttttcaa gatgttcatc acatcgatat
     6181 attgttgcca tgttgctgca tcaagtgttc cggctgtgac taagtttgct aaaactgcgg
     6241 caatccctgc cacgatccca gtaccgacaa atgcaggaat cattggcaca aagatattag
     6301 aaatgtcttt taataccgat ttgattttag atggtttttg tttcgatttt tgtgccgctt
     6361 tcatttcttg tgcccgttcg tttacacggt cttttccaga tgctgatgaa tttgctgtag
     6421 ctgtacttac agaagaaagt ttttctccta atttcacgcc tgccaaatca accatttgct
     6481 gtgctacttt gttcactttc ccaggtccga tgatgacttg aagctgttca tcttctacta
     6541 caccaagcac tcctggaatc gcttttaatt cagagacatt tacaagatca tcattgatga
     6601 tcttcatacg cacacgagtc atgcagtgga caatgctttc tacattgccc atgccgcctg
     6661 cccctttata aatttgtttt gccagttctt ccaaagtcag ttcgtttttt gccatggtct
     6721 tttccccctc ataaggtccc ttttacaaat ccgtttgctt cagctaattt tgcttgagct
     6781 tcctctttat cactatttgt caaaatcatg acgatagcca gcttaacatt ttgatctgct
     6841 tcttcaaagt acttttcagc tgtttcgtaa tcagcttctg tcgcttgcat gatgattcgt
     6901 tttgagcgtt cgattagttt ttcatttgtt ggtttgacat cgaccatcaa gttattgaat
     6961 actttaccta taccaatcat actgatagta gaaatcatat tcaagataag tttttgtgct
     7021 gttcctgact tcaatcgagt agagcctgtc aaaaattctg gacctgcatc gacttcgatt
     7081 gggaaatccg cgtgtttact gatttcggct tgtttattgc aagaaatcgt tcctgtgaca
     7141 gcaccgatcg attttgcgta atctaatcca ccgatcacat atggtgtacg accgctagct
     7201 gcgataccaa taactatatc attttttgtc aaattcagct tgattagatc ttcttgccct
     7261 agctcataag aatcttcggc cccttcgaca gctacagtca ttgcttcttc tccaccagca
     7321 attagaccta tgaccatttc cggttctaca ccgaatgtcg gtacgcattc agcagcatct
     7381 aacacaccta gtcttccact tgttccggct cccatataaa tcagacgtcc acctttttta
     7441 aaggactcaa tcgtgttagc aatcactggt tcgatacttg tcagttcttt ttcaaccgca
     7501 gcggccactt tctgatcttc tttgttcatt agttgaacgg cttgagaaat actcatctca
     7561 tctaatccaa aagtttcttg gtttctgcgt tccgttgtta ggttctctaa attgattttg
     7621 ctcattttta tcttccttct tttcttataa attgatagtt cataccagct ttgatttggt
     7681 tgatcaatgg tagctcttct ttgataactt tagctactac attcactttt tcatccgctg
     7741 gcaaatcata tttggttaat tggatctcac ccatatagcg caaatacttt tcgttatcta
     7801 aagtaataga ccctttcata cgcgtagctg tatttcttgc tgggataggc ggaatctctc
     7861 gaaaacgtgc atctacgctt cgtatgacat ctcttgcatc gtcttgccga ttcgtatgct
     7921 tccctaatat atattcgtaa aattcttcat ctacaggttc cgtatggagc aagactgtct
     7981 gttctttttg gaaagaagca aattgctcct gaacgttttc tgacagacct gcatctccga
     8041 tgtaaacaat atccgtgtta cttgctaata gatctagagc gcctgcaaga ggatggatcc
     8101 ctcgatgttc ttccaaagtt ggcaatcctt gatatagtgg gcctctcaac tctgtatctc
     8161 ctggtacaaa accttggaca aggagacctt gaattttgag ccattgattt tttttctgat
     8221 accattcttt gtccagccct gtttcgggac gtggataata attatgccaa gcttcaatct
     8281 gtgagaaatc agcttctgct tctttcaatt catcgatatc tttaggcgtg atcgtgctag
     8341 cattcaaact gattttcaat tgatgtgacc agtcggcaat ctgctgattg gaaatatgat
     8401 aatccattct taaacctgtt acaccaattg ctttcaaagg cgttagatct tctaatgaaa
     8461 aacccgctcg ttccaatgcc tctcctgaaa tatcgaccat caattctagt ttattctttt
     8521 tcgcttcgct tcctagttcg atcaatcgct tttgatatgc cgaaacatca tcttctggta
     8581 tatgcatgga agtgaaaata ccgacaaagc cattggatgc catttggcgg atatactgtt
     8641 tcttctcttc tgacaaagaa tcgttcataa atattgaaaa gccaaacata ttatccctct
     8701 ttttctgtat ccgtcttcat ttctatgact agagtatata tctaaagaaa caaaatttca
     8761 atacttttat taaaataatt aaatttcatt tctaaaggtt gagtttatat tatcaaaaga
     8821 attgtcataa cctaaaaaag gcactgctaa gcaaaagcag tgccttgaaa tattttattt
     8881 aaataacaca aaaacataat tactcataag aaaccaattc tgtattatct accatgaatg
     8941 taaagaaccc ctcattaggc atcggatgaa aataagcatt gatcactctt atcaaggcat
     9001 tatggcaaac taacagatag acattttctt cgtcttcgat gcattctttc aataaaggaa
     9061 cgatccgtgc atacacatct aagactgact ccccatttgg gaatcgtaca gcgaacgcta
     9121 aacgtgcttt ttgaaagttc tcatctttgc ttggcagtcc atcataatcc ccaaaatcca
     9181 tttctaccaa tcgttcatca acggtcagcg gcaagctgag acgatcagca actgcttgtg
     9241 ctgtatctct tgctcgttga agaggggaat ggatgatttt cgtgattgga acaggcaaat
     9301 ctactacctt ttctgctagt ttctgtgcct gcatctgtcc tttttcagtc agcggcacat
     9361 ctgcccgtcc gcaaactaat ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat
     9421 aaagcatgtg attctctcct tcgttggcaa agttcccctt aaccatacct tgttgttctt
     9481 gagattacaa gggttagatt ctctcaagag aaataatctc agtagatact ttatccagga
     9541 aggtttgatc atgttccact agtaacatgg caggtttcac gttcaagatc agttgttcta
     9601 attgttcttg attgaagaca tccaaataat tcaatggttc atcccatgta tatagttcag
     9661 ctggctgtga taaagattta gccaattcca cttttttccg ttggcccata ctcatctgct
     9721 cgatcttgtt atgaaaaaca tctctttcca tcccaagctt tcggaggttg ttcaaaaatg
     9781 cttggtagtc tacttggttt ttctctgcaa attccgccaa cgttcctcga ttgtcttcat
     9841 aattttggct tgcataacta atgctcagat gttttggctg gtatttttct cctataacct
     9901 tgccgttgaa tgcccccaga agataatgga taatggatga ctttcctgca ccgttaggac
     9961 ctgaaatcgc cacccgctga tgaggctcga ttgtaaaatg aattggctcg aataacagat
    10021 tttcataccc taattgaaga tcttctacgc ttaaaagtcg cttatggtga gacgcttggc
    10081 tattcatcgt caaagaatcg atatattcga tatcttttaa tagtttttct ttctcgctga
    10141 tctgggtctc catccgattc acgattgctt tggatcgttt catcgtccgt gcagcatcag
    10201 cacccactgc tcctttattc actcgtctag attcagtatc gatgaatccg acttgtttct
    10261 ttgttttatc tccttctcgg gaacgagacc attcggcttt ttcagctgct gttttcttta
    10321 gcctactgac ttcttttttc aatttttcat tttgagccat ttcaaattca tcacgaagtt
    10381 ttttctgttc ttcatagata gagaaattcc cttgataaag ttccagttga cttttttcga
    10441 ttgctaaaac atggtccact acttcatcga taaatccccg gtcatggctg accacgatga
    10501 agccttgttt tttctttttc aaataagccg ctacttgttt tctaccagag atatccaaat
    10561 gattcgttgg ttcatcgatt aacgggaaat gagtgtcatc cacaaataaa agtgccagta
    10621 ggacttttgt cttctctccc cccgatagtg tgctgaattc tctccataag atttcaggat
    10681 ctgtttgcat caattggagc tctctttcga tttcccatat ctcaaaatcc gtaatatcat
    10741 ttaacacgta ataggttaaa cgttctttat cttttgtctg ttgcgggaaa taggcaaatt
    10801 cttgctgatg gattacttgc ccttgataag gtagtttgtt ttgtagaata ttcagtaaag
    10861 ttgtctttcc tcgaccgttt cgtccgataa gtcctagttt ccattgtgtg tcaaaattta
    10921 gatttgcttg ttcaaataat aatgtgcctt ggctgtcgta gccgaatgtc agatttttta
    10981 tttcgatttt agacattttc catcacctcg taaaaaaaga ccagtcctat tttcagaact
    11041 ggctaaagag gatgcaaggg ttggatacac acaaaaagac ccatacatcg aaaaaaccaa
    11101 gccaatcccg aaaatctgca cactgcttcc ttgtctccac aaggtacagt ctgaatgcag
    11161 ctttcatcaa tcgacttaat ttttcaatgc acggatccct cactttgtcc tatatttgaa
    11221 attacgatag cataaagaaa atgattttgt caactacttc gggaggatgg tgaaaaatct
    11281 gctaagtgtc tgtcctaaga tttttgtttt ttctaatgtt tgcctgtgtt ccattgcttc
    11341 ttctaaacct attggaccca aatgaatact taagacaatt gggagaaaat cagaaagtcc
    11401 ttctagctct tggtctctac ttccgcaaat agcgattatt ggttttcgat atttttttgc
    11461 aagcactgct acaccgtatg gaacttttcc gtggatcgtt tgctgatcga tccgcccttc
    11521 tcctgtaatg atgacatctg cttgttcgat ctttttttcc aatccagtga tttggctgac
    11581 taaatcgaaa ccagaaacca tcttcgctcc caacaaaaaa agggctccac caataccgcc
    11641 tgctgctcca cttcctggaa tatcatctaa atccacctcc cagaaatctt ttatttgctg
    11701 agcaatcttt cctgcttgtt tatctaagaa agctagcgtc gtatctattc ctcctttttg
    11761 tcttccaaat tgccaagcag ctccttgttg acctgtatat ggattggtaa catctgcagc
    11821 gataatcaat tttactttag aaacaagaac gtttcttcga tcaaaatgaa gttttttagt
    11881 agataataaa ggatttcctt ctgtgtaccc ttctaaagaa gcacccaagg attggagtaa
    11941 tcctagacca ccatcgctac acccgcttcc tcctaaagtg acaataattt gttctacttc
    12001 ttgaagcagt gcatctttaa taagttctcc taaggcataa ctggaagcaa gtcggatagt
    12061 ttgatccgtt ggtttatcaa gtagatctaa ccccaatgct tgtgcagatt cgatgactgc
    12121 ggtgtttttt ccttgccaag ttgtcaataa atagctgatt tttcgctgac ggaataagag
    12181 atccatccca tcaatggtac gccatgttcc cctgagcgcg taatagattg cttctgcagt
    12241 attttctccg ccatctgcaa tcggcacatt gatttttctt cctttacgat cttcccagcc
    12301 atttagaact gcctgattta gttcaaatga agaagcactt cctttaaatg aatcaattgc
    12361 cgccaaaata tccatctgta tccctcactt tcttcttaag taagacactt tttttcattt
    12421 ttttcaaaca aaacaatgct attttcttcc atttttctat atactaaaag ggaactcatt
    12481 gaataaagga gagaaaaaac atgaaaatcg caatagctgg ggccggagca atgggcagcc
    12541 gaatcggatt gatgctacat caaagcggaa atgaggtcct cttgatcgat cgttggcctg
    12601 ctcacattga agccattcgt acaaatgggt tgattgctga ctttaacggt aaagaagtgg
    12661 tcgcaaaact gccaatctat tcaccagaag aaatcatcga aagcaacgaa cacgtggact
    12721 tgatcgtagc attaacaaaa gccaatcaat tagatgatat gttttgttcg atccaatcca
    12781 ttattactga caatacttat gttttgtgtt tactaaatgg attaggtcat gaagatgtac
    12841 tggaaaaata tgtgccaaag aaaaatatct tatttgggat cacgatgtgg acagctggtc
    12901 tagctggtcc cggaaaagtc acattgttag gtgatggaga aatcgaacta gagaacttgg
    12961 aaccagaagg cgaagcattt acaaaaaaag ttgtagaagt ctttcaagaa gcaaatttaa
    13021 atccgatcta tagccataac gttcgttatt ccatttggcg aaaagcttgt gtaaatggaa
    13081 cattgaatgg tttatgcacg attttggatt gtaatattgc tgaactaggc gcgcaaaaag
    13141 cggcagagag catggttcga acaatcgtat ctgaatttgc ttctatcgca gcaaaagaag
    13201 gaatcatact agatcaagaa gaagtgtacc aacatattgc ttctacttat gatcctgaca
    13261 acatcgggtt gcattaccca tctatgtacc aagacctgat caaaaaccat cgtttgactg
    13321 aaatcgacta catcaatggt gccatttggc gaaaaggaca aaaatatgat attgctacac
    13381 catattgtgc ctttttgacc caattagtcc atgcaaaaga aggaatcctt ggcgcagaat
    13441 aaaaggctct ctgatgagaa tcagctgaat cagcagctgg ttctcttttt ttaagcccaa
    13501 taaaagatca aaaagaaaac acaactctca tgatgccata agaattgtgc ttgtttgttt
    13561 cagtacataa tctcttcaaa atgtaggttt tttatttgtt ttcaacagag gagaggtcat
    13621 attcgtattg ctcataaata gtggaatcgt tagtgataaa tcgttttgcc caattttctg
    13681 catcataaaa ttccaatagc tctgatagga ttggcttaaa tggtgtatgc agaaattccg
    13741 gtaattcatc taaatcacgt tctcgaagca aaaaaatatt ttcaggacga taaaaatgat
    13801 aatgagcaat cgcttgatta tccgtcacta gtttcttttg aaaatacatg gattcaaatg
    13861 tccgcaaagt cactccttgc tgaccctccc gcaacagttc cagaatcccg cgcgattccc
    13921 ctgttttttt caggtactct tcataagtaa gatatccagg ttgttcattc cctcgatgtg
    13981 gaagaatgaa atagttggtc gtaacatctc tttttataaa ttcctgctta tagctttctg
    14041 ctcgttcttt tctcccatta ttcgttgcac caaaaaatag atccgtcttt ggttcgctag
    14101 aagaaaaatt catatgtttc gaataaaatg aactattatg tttcagccca agttcttttg
    14161 cttccaaaaa atcaaaatga taaatctcat cgattgccgg ttcatcttgt gcctgcttca
    14221 gcatcgcctc gtgttctgct ttgaagtgat tccaaaaaaa taacacaatt tttgcattgg
    14281 taaatgggcg cacgcgtcga gccatctcca tatcaaatcc ataatctaaa aaaacgatca
    14341 gctgataatt tgccaactgt tgtctagctg atttaatgaa ttccgactgt agtaaatctt
    14401 ttccggacca aacatctaaa gagcggatgg aaggcaaaaa ataaagtgat ttattttttg
    14461 caactagcag tacgtcattt ttgttcatct tttccgcctc actttctctg tcactttttc
    14521 ctatgatagc ctgtttaaat agtaaaaagc cagtaaaacg tttcctatat ttgtatgaaa
    14581 atttaacatt tgtatgaaaa tatgatgaaa taaatcattc ttctattaaa attcgcctaa
    14641 aagttttctg aatagttgta attgtctgaa aatttgttat cattagtcat tgttaaatat
    14701 tgctatttat tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg
    14761 aagataaaaa attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg
    14821 gagcaatcgg tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt
    14881 ctgtattgat tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg
    14941 gaaaaatcgt tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt
    15001 atctaggtgc tcattctgct gattttactg attgggtcta ctggtctatg tggattatca
    15061 atatcatcgc tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc
    15121 cagcctgggt atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg
    15181 tacggttatt cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct
    15241 tactgatcat ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc
    15301 ctacccttca gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca
    15361 tcagttcttt gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg
    15421 taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa
    15481 gaatcatttc cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata
    15541 ctttagcagg cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat
    15601 ttgcagcgga tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg
    15661 gtgtgtatgc atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta
    15721 gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca
    15781 gtgttcttta tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc
    15841 tggcaggttc cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct
    15901 ttgtactggc actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag
    15961 ctgtcttagg attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat
    16021 tgactggcct gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg
    16081 tattgacgaa tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag
    16141 ctattgaaga aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag
    16201 tctcatgacc tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc
    16261 tccatgctca tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa
    16321 aagtattgga agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg
    16381 tacttgaatg tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg
    16441 ttgatgatgg gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa
    16501 aacaaaaaga taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg
    16561 tttatggtac gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct
    16621 catcattcta tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa
    16681 aaattagcgg aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg
    16741 atcaaagaaa tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt
    16801 atcagcttca agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa
    16861 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc
    16921 gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt
    16981 aaaatcagcg ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt
    17041 tgccattttt tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg
    17101 tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt
    17161 ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag
    17221 ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct
    17281 gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag
    17341 gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg
    17401 agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag
    17461 tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct
    17521 gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca
    17581 ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg
    17641 ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac
    17701 aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt
    17761 cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat
    17821 tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct
    17881 actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac
    17941 agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga
    18001 acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc
    18061 atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg
    18121 tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga
    18181 gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt
    18241 aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag
    18301 gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac
    18361 tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc
    18421 catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg
    18481 atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac
    18541 aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca
    18601 acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt
    18661 ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa
    18721 agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa
    18781 aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga
    18841 tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat
    18901 taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg
    18961 cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga
    19021 aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa gtttattttt
    19081 tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg
    19141 attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct
    19201 gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct
    19261 gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact
    19321 tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct
    19381 tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta
    19441 acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt
    19501 atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga
    19561 tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact
    19621 cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg
    19681 ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct
    19741 gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat
    19801 tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca
    19861 aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct
    19921 ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg
    19981 gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt
    20041 aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt
    20101 gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct
    20161 aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg
    20221 tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat
    20281 tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat
    20341 tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact
    20401 ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca
    20461 tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct
    20521 aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc
    20581 actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg
    20641 tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta
    20701 tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca
    20761 tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta
    20821 gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt
    20881 tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca
    20941 tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg
    21001 ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc
    21061 cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct
    21121 gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt
    21181 tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa
    21241 aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt
    21301 ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt
    21361 agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc
    21421 ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc
    21481 ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg
    21541 aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa
    21601 gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat
    21661 attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg
    21721 tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca
    21781 aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac
    21841 attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga
    21901 tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat
    21961 aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta
    22021 acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt
    22081 tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca
    22141 ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg
    22201 cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc
    22261 tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct
    22321 tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta
    22381 actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg
    22441 cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg
    22501 ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca
    22561 aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag
    22621 aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat
    22681 gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac
    22741 tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca
    22801 cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt
    22861 tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac
    22921 atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct
    22981 caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt
    23041 caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt
    23101 cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa
    23161 tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta
    23221 cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg
    23281 tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc
    23341 ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc
    23401 ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc
    23461 accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata
    23521 ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg aaattcaaat
    23581 tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac agttcgaaga
    23641 tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata atttcggaca
    23701 aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc tgttgctcct
    23761 actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg ttccaaagct
    23821 gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg
    23881 tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt cgtaacagtg
    23941 gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat ttcttttgca
    24001 tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc ttcacgcttc
    24061 gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat atcaaaactt
    24121 tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg gatatcaaat
    24181 tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac taacaaacca
    24241 tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac aatacacata
    24301 gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc gtttataaga
    24361 gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga
    24421 tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag cctaattcag
    24481 caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta gtgataacaa
    24541 acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc agtgcatgga
    24601 gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat aatgcgatga
    24661 aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca gcaggacaaa
    24721 taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt
    24781 aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc aaatattctt
    24841 tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca
    24901 aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa
    24961 acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata ttcccaaaag
    25021 tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca
    25081 cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg atatcagcca
    25141 ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta tgaccatttt
    25201 tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg aatacttcat
    25261 ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc tcaccggtaa
    25321 ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact aattcgttgt
    25381 ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac gcttgtttcg
    25441 ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg tctgactcat
    25501 ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca ccaatcactc
    25561 gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt cgttcttttg
    25621 ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc atttcttcaa
    25681 tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca acactcatac
    25741 gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc ttatctgtat
    25801 ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt
    25861 gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt ccttcacaat
    25921 ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta tccgtacata
    25981 acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact gttccttttt
    26041 tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag
    26101 gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa tccccttttg
    26161 taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg atcagtagca
    26221 tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca gatagcgttt
    26281 ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct tccgcacttc
    26341 cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg ctttgctgat
    26401 cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt
    26461 gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc atatcccctg
    26521 ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct gtgtactttc
    26581 cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca gccgcttgat
    26641 tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata gtgaagataa
    26701 tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc gtgatgaatg
    26761 aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct ttcaatattt
    26821 ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg
    26881 aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt tcaaataatt
    26941 cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca
    27001 tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg tctttagcta
    27061 ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat aatagccctg
    27121 gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct catctccttt
    27181 actaagtaat tttaattcat gacttccaat catttttcga atagttattt ttccaattag
    27241 accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa cctctcgcca
    27301 aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg
    27361 agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct tatgaaaagc
    27421 cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg ggcagtagcc
    27481 tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg ttaatataac
    27541 aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt ttaaaataca
    27601 cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc ccgctataat
    27661 agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt atcaagccag
    27721 acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca aagggactgg
    27781 ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa cattatcagc
    27841 acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc ggtcctgtcg
    27901 tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa
    27961 caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg ccggggactg
    28021 aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc gcccgctttt
    28081 tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa
    28141 ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa ggacctccgt
    28201 tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt
    28261 tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa tgtttcatag
    28321 atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt cacactacgt
    28381 gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa aaaagtaaag
    28441 agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt gattttcaaa
    28501 taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag aatagatagt
    28561 tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg gcattcataa
    28621 cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg aagattcaaa
    28681 accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct gatctcttta
    28741 taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt ctttaacttt
    28801 attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat gttcattcgc
    28861 tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt ctcaatcccg
    28921 actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa cttcttgttg
    28981 actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
//
LOCUS       DAZUKR010000022        28189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000022 DAZUKR010000000
VERSION     DAZUKR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.022"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..907
                     /locus_tag="ACG89C_000877"
     CDS             38..907
                     /locus_tag="ACG89C_000877"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4899452.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1072..2343
                     /locus_tag="ACG89C_000878"
     CDS             1072..2343
                     /locus_tag="ACG89C_000878"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4899453.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2340..3635
                     /gene="rho"
                     /locus_tag="ACG89C_000879"
     CDS             2340..3635
                     /gene="rho"
                     /locus_tag="ACG89C_000879"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4899454.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3734..3997
                     /locus_tag="ACG89C_000880"
     CDS             3734..3997
                     /locus_tag="ACG89C_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4899455.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4442..4891
                     /locus_tag="ACG89C_000881"
     CDS             4442..4891
                     /locus_tag="ACG89C_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4899456.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4948..6366
                     /locus_tag="ACG89C_000882"
     CDS             4948..6366
                     /locus_tag="ACG89C_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4899457.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6366..7226
                     /locus_tag="ACG89C_000883"
     CDS             6366..7226
                     /locus_tag="ACG89C_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4899458.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7400..8173
                     /locus_tag="ACG89C_000884"
     CDS             7400..8173
                     /locus_tag="ACG89C_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4899459.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8320..8685
                     /locus_tag="ACG89C_000885"
     CDS             8320..8685
                     /locus_tag="ACG89C_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS4899460.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8833..10041)
                     /locus_tag="ACG89C_000886"
     CDS             complement(8833..10041)
                     /locus_tag="ACG89C_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4899461.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10060..10338)
                     /locus_tag="ACG89C_000887"
     CDS             complement(10060..10338)
                     /locus_tag="ACG89C_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899462.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10531..11187
                     /locus_tag="ACG89C_000888"
     CDS             10531..11187
                     /locus_tag="ACG89C_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4899463.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11629..12426)
                     /locus_tag="ACG89C_000889"
     CDS             complement(11629..12426)
                     /locus_tag="ACG89C_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4899464.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12423..13607)
                     /locus_tag="ACG89C_000890"
     CDS             complement(12423..13607)
                     /locus_tag="ACG89C_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS4899465.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13632..14879)
                     /locus_tag="ACG89C_000891"
     CDS             complement(13632..14879)
                     /locus_tag="ACG89C_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4899466.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15094..16839
                     /gene="dnaX"
                     /locus_tag="ACG89C_000892"
     CDS             15094..16839
                     /gene="dnaX"
                     /locus_tag="ACG89C_000892"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4899467.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16859..17173
                     /locus_tag="ACG89C_000893"
     CDS             16859..17173
                     /locus_tag="ACG89C_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4899468.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17198..17794
                     /gene="recR"
                     /locus_tag="ACG89C_000894"
     CDS             17198..17794
                     /gene="recR"
                     /locus_tag="ACG89C_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4899469.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17858..18502
                     /gene="tmk"
                     /locus_tag="ACG89C_000895"
     CDS             17858..18502
                     /gene="tmk"
                     /locus_tag="ACG89C_000895"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4899470.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18517..18846
                     /locus_tag="ACG89C_000896"
     CDS             18517..18846
                     /locus_tag="ACG89C_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS4899471.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18860..19798
                     /gene="holB"
                     /locus_tag="ACG89C_000897"
     CDS             18860..19798
                     /gene="holB"
                     /locus_tag="ACG89C_000897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4899472.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19834..20658
                     /locus_tag="ACG89C_000898"
     CDS             19834..20658
                     /locus_tag="ACG89C_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS4899473.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20651..20998
                     /locus_tag="ACG89C_000899"
     CDS             20651..20998
                     /locus_tag="ACG89C_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS4899474.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLWEHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21067..21939
                     /gene="rsmI"
                     /locus_tag="ACG89C_000900"
     CDS             21067..21939
                     /gene="rsmI"
                     /locus_tag="ACG89C_000900"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4899475.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22048..23169
                     /gene="dinB"
                     /locus_tag="ACG89C_000901"
     CDS             22048..23169
                     /gene="dinB"
                     /locus_tag="ACG89C_000901"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4899476.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23223..23825)
                     /gene="nrdG"
                     /locus_tag="ACG89C_000902"
     CDS             complement(23223..23825)
                     /gene="nrdG"
                     /locus_tag="ACG89C_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS4899477.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24140..26329)
                     /gene="nrdD"
                     /locus_tag="ACG89C_000903"
     CDS             complement(24140..26329)
                     /gene="nrdD"
                     /locus_tag="ACG89C_000903"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4899478.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26655..27422
                     /locus_tag="ACG89C_000904"
     CDS             26655..27422
                     /locus_tag="ACG89C_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899479.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27412..>28189
                     /locus_tag="ACG89C_000905"
     CDS             27412..>28189
                     /locus_tag="ACG89C_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899480.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFC"
BASE COUNT         9027 a         5090 c         5919 g         8153 t
ORIGIN      
        1 caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat caggagctga
       61 atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca caaacaactt
      121 ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag tacttatcca
      181 aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag atttaatcac
      241 tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag accatggtga
      301 ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt tcgatggttc
      361 acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa aagcacatgc
      421 aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag acggaatcat
      481 cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta caggtatcga
      541 ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact ggaaaggatt
      601 agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc ctttagtatt
      661 acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa tgggtatctc
      721 aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc gtaaatatat
      781 cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat tagctccagg
      841 taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt cagctaacaa
      901 agcgtaagca tccacataac agatacgata tataggaatg gagaacgact tttgaagtcg
      961 ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt ttatctatga
     1021 taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca gatgaaaaaa
     1081 atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg tgcaaaaaat
     1141 agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat cttggaaggt
     1201 gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat gggcgcaact
     1261 gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc gattccgatg
     1321 ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac actgcttggt
     1381 aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc tagaccgatt
     1441 gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga acatggtgcc
     1501 atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat ggacgtggtt
     1561 tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg acagacagta
     1621 attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact gaataatatg
     1681 ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt cgaaacatta
     1741 catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta tctagcatta
     1801 gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca tttggaaagc
     1861 tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga tgaaatcgaa
     1921 gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta tccaggattt
     1981 gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg cacttcagaa
     2041 atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc acgaatgggc
     2101 gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa acttcacggc
     2161 acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc tggactattg
     2221 gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta tgatcatatt
     2281 atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt gggagctgaa
     2341 tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa gtttatagtt
     2401 atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa ttatctctag
     2461 ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga atattggata
     2521 tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca agtgcagaag
     2581 atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg gacaaagtgg
     2641 caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg cacgtggaaa
     2701 gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca gccttaacac
     2761 ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta tctacccgaa
     2821 tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc gcaccgccaa
     2881 aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa aactatccgg
     2941 aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg gatctagaac
     3001 gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa aaccatactc
     3061 gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa cgagatgtcg
     3121 ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc gttccaccaa
     3181 gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct aagaaatttt
     3241 tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg accgctttag
     3301 tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga actggaaatc
     3361 tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc atcgatatca
     3421 aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta gaagaaattt
     3481 ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa ctgatccgct
     3541 ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat gtctcctttg
     3601 gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg aaacatgtta
     3661 agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag cagggcaaag
     3721 gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag tattcatgga
     3781 ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag aaacagttga
     3841 atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg actcacatcc
     3901 attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc gtttcaacaa
     3961 aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat aactcaacgt
     4021 ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa tttaaaactt
     4081 cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa actagtatat
     4141 agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga ggggttattt
     4201 cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa ggaaggcttt
     4261 ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta atgacttata
     4321 cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt acagctagcg
     4381 atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg agaggttttt
     4441 tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat cgatagattc
     4501 aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata aggtaagaaa
     4561 tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca caggaataga
     4621 agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag cactgtgttt
     4681 ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa ttgaatatat
     4741 ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc tcagcacact
     4801 ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca ctacgccagc
     4861 agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc cgagtatcga
     4921 tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc ttgcccgact
     4981 ggaattgctc atacttatat gtcggcagaa aaattagaag taacagcaag aaatctaaac
     5041 catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc tgaacaagaa
     5101 atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt ggaccgattt
     5161 gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc gcaagtagta
     5221 atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa agaaccagct
     5281 gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa ttacatgctt
     5341 ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg gattacagcg
     5401 gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag gatcggagga
     5461 gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac atcgattgct
     5521 ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac aggtggttca
     5581 ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga ttttctggca
     5641 aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt cgtccccttt
     5701 ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc gatcacatgg
     5761 gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg gctattgttc
     5821 ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa taaatcgact
     5881 tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc tgcttgtatg
     5941 gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt caaacataaa
     6001 tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc ttcgtatatt
     6061 acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc tagtttgatg
     6121 acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc aatggcacct
     6181 catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc tatgtatgtc
     6241 ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt gaaaaaaaca
     6301 gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa tgaggtggaa
     6361 gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat caatttgcag
     6421 taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa gaagcagaga
     6481 gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat ttaagtgatg
     6541 agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc gttatccact
     6601 tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc tattcttccg
     6661 tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg aaaaaagtgg
     6721 ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg attgggaata
     6781 atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat caagcgaaga
     6841 tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca gcgcatggct
     6901 tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa ttgaaccaac
     6961 aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca gaagtcagcg
     7021 aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa agcgtctttt
     7081 tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg aatgcaattt
     7141 atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa attttgaaca
     7201 cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg gtatgacaat
     7261 agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt cgaaaaattt
     7321 cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg ttactatata
     7381 aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg agattgtgaa
     7441 ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag taaattatag
     7501 cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga tccgtagagt
     7561 tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc tgatgaagat
     7621 caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt cttctttgat
     7681 tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact tagtcagaaa
     7741 cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag ctgctatgtt
     7801 gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc cgaaacgagc
     7861 aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg atttagcgat
     7921 aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg aaggagaggc
     7981 aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag ttacacagca
     8041 aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg attatctggt
     8101 gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc atatcgaatg
     8161 tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt tagataggac
     8221 cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta tcttgttaga
     8281 taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt gaaggcgttg
     8341 gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg tttatacggt
     8401 gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat ctatgtgcta
     8461 ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc agatgctggt
     8521 tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt cgatatttgg
     8581 atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt ccacaagtgg
     8641 ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa cagttaaact
     8701 attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag aataatcata
     8761 aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt tttttttatc
     8821 tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct ctgcttcatg
     8881 actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa tgactccggc
     8941 aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca aataaggacc
     9001 aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac tgattcgatg
     9061 ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac cataacctag
     9121 gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac tcagtaaaaa
     9181 taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc ctttgacatc
     9241 gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata cgacgaagaa
     9301 aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa atgataaaac
     9361 acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg ctttcttttc
     9421 aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag ataatggaat
     9481 attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta caattgaaaa
     9541 ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa atggcccaac
     9601 agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt ttttagatgc
     9661 agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt agccgaatcc
     9721 attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat atgctaatgt
     9781 tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa atagttctaa
     9841 ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc cagaagcaaa
     9901 acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca tgatgaccat
     9961 caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa taaaatattt
    10021 attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat atgcttttaa
    10081 tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa caagttggcg
    10141 aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt cgtttgttgg
    10201 attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat ttaaaaagac
    10261 taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat taatgattgt
    10321 gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact tctgcagtat
    10381 aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact taacaaatgt
    10441 taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg tatgatcttt
    10501 ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa ggaatatttg
    10561 gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac attcgaaggg
    10621 atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag tgttatttca
    10681 aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt ctctctttta
    10741 aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga atcagaatat
    10801 gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca agacccaaag
    10861 ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat gaaaaaaatg
    10921 cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta cgaattatat
    10981 gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat cacgaatgaa
    11041 gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat tttaaaacaa
    11101 ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt gaatatggac
    11161 ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca gcgttttgac
    11221 ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta
    11281 ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac
    11341 tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact
    11401 tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa aagctgttct
    11461 gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa cttggctgga
    11521 cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag gggctgtgac
    11581 acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt agaaacttct
    11641 gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc caccgccgct
    11701 tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg tcagacgatc
    11761 ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt agatgcctga
    11821 tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga taagagccaa
    11881 aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt
    11941 ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg ttgctgattt
    12001 agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat cataaacatc
    12061 atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac cagaagtcga
    12121 aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt gatcatttcc
    12181 gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat tagatgtagt
    12241 cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct gttgttccaa
    12301 agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag ctgtatctgc
    12361 atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa aaaacttttt
    12421 catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa aatcccaaga
    12481 gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt tcctgtttgt
    12541 ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata agtaacaagc
    12601 cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga agttcgcaca
    12661 ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga atagtctttt
    12721 aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt ttctgcttgg
    12781 aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa aggttgcacg
    12841 ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag ttctttaaaa
    12901 gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct
    12961 atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac ttcagcatct
    13021 gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt attgaaaaaa
    13081 gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat tgcttcttct
    13141 ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa acgtcccgat
    13201 agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg
    13261 atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt atctgcagat
    13321 tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc
    13381 atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct
    13441 ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg gctgacttct
    13501 tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt gggatcaaaa
    13561 gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag ctgcacacct
    13621 ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt tggacagaat
    13681 ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg catttgtagc
    13741 ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa ttttccggaa
    13801 ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc agtttgttga
    13861 ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc ttgagaaaga
    13921 taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat tgctcctgct
    13981 tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta tccttcacgt
    14041 acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt cacagaatca
    14101 tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg taattcacgc
    14161 caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt agatggtaca
    14221 taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag cgctgttagg
    14281 aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt gtttttaggg
    14341 attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt gccatgtgcc
    14401 cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg
    14461 aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt ctgcggtgtg
    14521 acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag tgttccattg
    14581 aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc ggtataatcg
    14641 atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg gacgactgtt
    14701 ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc atctttgcgg
    14761 actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc gatgacttct
    14821 agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat aagacccata
    14881 aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta ttataccata
    14941 ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg cgtattgttt
    15001 aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac gtataataag
    15061 agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact ttatcgagtc
    15121 tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa
    15181 aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc cagaggaacc
    15241 ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa cagtaaagac
    15301 ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat
    15361 gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt tatccgtgat
    15421 cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga tgaggtacat
    15481 atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc aaaagaaagt
    15541 gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat catttcacgg
    15601 actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca tcttgaattt
    15661 atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat tgcccgttct
    15721 gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc gtttagtgaa
    15781 ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga cgaaatgatg
    15841 gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga aacattgaac
    15901 caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt acaatactgc
    15961 cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc gagtacgatc
    16021 agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt ttcaatgata
    16081 aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc tatttatttg
    16141 gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc agcagatgta
    16201 gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga actaagaaac
    16261 gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca gccggtacag
    16321 caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg agtatataaa
    16381 gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg ggaagacttg
    16441 ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc agttgcagct
    16501 ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag agcagcaaat
    16561 gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa agattatgcg
    16621 cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa ttatttgacg
    16681 caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga aggagaaatc
    16741 gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa agcagaggaa
    16801 ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt agataattat
    16861 gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga
    16921 aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag ccaccaatga
    16981 attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga
    17041 agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga
    17101 tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg caaaaggttt
    17161 gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag aaccaattgc
    17221 gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag cgacaagact
    17281 cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta aatctttgtt
    17341 aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag aagaagatcc
    17401 ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc
    17461 gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc atgttctcca
    17521 tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt cttctctgtt
    17581 gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg caacaacaga
    17641 gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta tcaaagtgac
    17701 tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg aagttacttt
    17761 attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt tttttcagat
    17821 acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat ttattacaat
    17881 cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc ctcttttgaa
    17941 agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc cgattgctga
    18001 agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac gtacggaagc
    18061 actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc ctgctctggc
    18121 aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc
    18181 aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta cagaaggtac
    18241 tacgccggat tttacccttt atcttgacgt agattcagac accggattaa gaagaatcaa
    18301 gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat tccaccagcg
    18361 tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc acaaagttga
    18421 tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta ttgaacagta
    18481 tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt agcgattatc
    18541 caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat ccgtgccaca
    18601 aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat cgtgggtatt
    18661 gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga atcaagaaag
    18721 caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt cccttatcca
    18781 gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg attccatcaa
    18841 ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt
    18901 gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt atctttttga
    18961 aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc acctgttctg
    19021 tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa
    19081 gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca aagtcgatca
    19141 gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa aaaaagtttt
    19201 tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt tgaaattctt
    19261 ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag gaagaattct
    19321 ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga aagaagcact
    19381 gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt tggctttctt
    19441 aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta atgatgctaa
    19501 ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag cctttatcta
    19561 tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta caggattatc
    19621 tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg
    19681 caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc agaaactacg
    19741 cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga ttttttagct
    19801 tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg tgtccgatat
    19861 agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt ttatagtgac
    19921 aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct tcctaaaaaa
    19981 gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa agcgtcacaa
    20041 gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca
    20101 aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga atacacattt
    20161 gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt tagagaattg
    20221 gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat tggtgtccgc
    20281 gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg ctgttcttct
    20341 tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag tttatctttg
    20401 aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca atatgagagc
    20461 gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat tgctacacct
    20521 gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag tattcggctg
    20581 actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt tatccaagta
    20641 ggtgagatga atggataaga gatcattata cgacggattg aatcaattag aatcagagtt
    20701 gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa
    20761 aaatacaact ttggagatgg aaaatcaacg tctgtgggaa cacctgcggg aattgaacca
    20821 agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct
    20881 tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat caagaaggga
    20941 caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc
    21001 aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt aaggagattg
    21061 tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga tttatatctt
    21121 gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg tatgctgcaa
    21181 gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt gaatcatttt
    21241 gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg tgtccctcaa
    21301 ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc aggaatgcct
    21361 tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg gatttctgtg
    21421 atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg gctagtgcct
    21481 caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa agaaacattg
    21541 aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta tcggatcact
    21601 gctacattga cgaatttttt agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa
    21661 ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact cgcttatatc
    21721 gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg
    21781 ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga agcaaagatc
    21841 gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa
    21901 aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag ttgtctataa
    21961 tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg cgaacgaatg
    22021 ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt acataatgat
    22081 acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt tgaagaacga
    22141 gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag tgacactggt
    22201 gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat tcattcagca
    22261 atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc tggcgaccat
    22321 cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta tacagatatt
    22381 gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc
    22441 aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag tgtccagcta
    22501 acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc agactatcaa
    22561 aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt aaaagctttg
    22621 ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca tgagctaggg
    22681 atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg aaattttgga
    22741 aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt
    22801 acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt gcaaacggaa
    22861 gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc actgagaaga
    22921 gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta ttctactgtg
    22981 accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt ttatcaagca
    23041 agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt agggattact
    23101 ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa
    23161 gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc ccaaagctgt
    23221 ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc cgtacatttt
    23281 tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat ccgctggttg
    23341 ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg tccgtcaaca
    23401 aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag caattcatca
    23461 aaggcatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt tcggatacgt
    23521 cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag taaagttaat
    23581 ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc aggtgtgaat
    23641 ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca ggcaaataag
    23701 cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt aaacggtttg
    23761 tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg ttttggattt
    23821 ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag ataaaatcta
    23881 ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa gcttgttctt
    23941 attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg aaaaaagact
    24001 tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc agtaataagc
    24061 ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt acttgatgca
    24121 ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt tctttatggc
    24181 ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta cgtttgacta
    24241 catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct cgttctgtcg
    24301 gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta ttcgtaccaa
    24361 gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc ggattttgtt
    24421 ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt ggataatcct
    24481 tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag tggaaactgt
    24541 ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct gtatccaaac
    24601 ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa tgataaccat
    24661 attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca agtgtgaaag
    24721 cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca acttcataca
    24781 atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca ttgacagaat
    24841 cggtacgtga aagacgtttc ccgaaagaac catacatata taaaattggc gcatttgctg
    24901 gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg atttctaagc
    24961 ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc gcgattcttg
    25021 gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct tgaccatttt
    25081 cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa cttccagtta
    25141 attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt gcacattcca
    25201 atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca ccttttttca
    25261 aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat ccattgattc
    25321 gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct agtccaaatc
    25381 ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt tcatattcca
    25441 aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat tctttttggc
    25501 gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag ttcaattctg
    25561 caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat tgacttgacg
    25621 ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat tttggcgatt
    25681 cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc
    25741 agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg atatcgcctt
    25801 tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat tttccaacga
    25861 tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt gcgttttcgt
    25921 tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata tctgttgctt
    25981 tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct aattcatatt
    26041 cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt ttgacatttt
    26101 cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga attttttcat
    26161 aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt gcttcattga
    26221 tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc atctgggatg
    26281 tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt atcttcaact
    26341 cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg
    26401 gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg tagtgtatat
    26461 tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc atatttaatt
    26521 ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag tgacaaaaac
    26581 ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg aaaaatgaaa
    26641 gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa agctacggca
    26701 gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa gaaggcgagt
    26761 ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat atcttgtcca
    26821 cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga
    26881 aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt caagagttca
    26941 atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca gttgaacgga
    27001 tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg aatatagaag
    27061 aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga attgcagctg
    27121 ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact ggctctttgg
    27181 actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa aaagaaaaag
    27241 caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat agaattttat
    27301 ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga agagagttct
    27361 ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg aatgctgtat
    27421 aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta ttttttcagt
    27481 ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga tcaatcttta
    27541 gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg tggatttatc
    27601 attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt gaatagaagg
    27661 caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat atcaggtata
    27721 tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat attactcggt
    27781 attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat ggatcttact
    27841 agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt tttcatcatc
    27901 cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat gattcgaagt
    27961 tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag gcaccgatta
    28021 ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag tcattttagg
    28081 gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt cttttttcct
    28141 tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtt
//
LOCUS       DAZUKR010000023        27948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000023 DAZUKR010000000
VERSION     DAZUKR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.023"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(388..1332)
                     /locus_tag="ACG89C_000906"
     CDS             complement(388..1332)
                     /locus_tag="ACG89C_000906"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4899481.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1628..2572)
                     /locus_tag="ACG89C_000907"
     CDS             complement(1628..2572)
                     /locus_tag="ACG89C_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4899482.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2793..4007
                     /locus_tag="ACG89C_000908"
     CDS             2793..4007
                     /locus_tag="ACG89C_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4899483.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4029..6044
                     /locus_tag="ACG89C_000909"
     CDS             4029..6044
                     /locus_tag="ACG89C_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS4899484.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6127..7500)
                     /locus_tag="ACG89C_000910"
     CDS             complement(6127..7500)
                     /locus_tag="ACG89C_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4899485.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7526..7810)
                     /locus_tag="ACG89C_000911"
     CDS             complement(7526..7810)
                     /locus_tag="ACG89C_000911"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4899486.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7812..9827)
                     /locus_tag="ACG89C_000912"
     CDS             complement(7812..9827)
                     /locus_tag="ACG89C_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4899487.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10006..10308)
                     /locus_tag="ACG89C_000913"
     CDS             complement(10006..10308)
                     /locus_tag="ACG89C_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS4899488.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10397..11428)
                     /locus_tag="ACG89C_000914"
     CDS             complement(10397..11428)
                     /locus_tag="ACG89C_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4899489.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11635..12510
                     /locus_tag="ACG89C_000915"
     CDS             11635..12510
                     /locus_tag="ACG89C_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4899490.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12662..13504)
                     /locus_tag="ACG89C_000916"
     CDS             complement(12662..13504)
                     /locus_tag="ACG89C_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4899491.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13596..15473)
                     /locus_tag="ACG89C_000917"
     CDS             complement(13596..15473)
                     /locus_tag="ACG89C_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4899492.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15470..16891)
                     /locus_tag="ACG89C_000918"
     CDS             complement(15470..16891)
                     /locus_tag="ACG89C_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4899493.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16894..20283)
                     /locus_tag="ACG89C_000919"
     CDS             complement(16894..20283)
                     /locus_tag="ACG89C_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4899494.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20540..21931
                     /locus_tag="ACG89C_000920"
     CDS             20540..21931
                     /locus_tag="ACG89C_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899495.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22004..22651)
                     /locus_tag="ACG89C_000921"
     CDS             complement(22004..22651)
                     /locus_tag="ACG89C_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4899496.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22659..23345)
                     /locus_tag="ACG89C_000922"
     CDS             complement(22659..23345)
                     /locus_tag="ACG89C_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899497.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23351..24424)
                     /locus_tag="ACG89C_000923"
     CDS             complement(23351..24424)
                     /locus_tag="ACG89C_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899498.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24646..25320
                     /locus_tag="ACG89C_000924"
     CDS             24646..25320
                     /locus_tag="ACG89C_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4899499.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25325..26665
                     /locus_tag="ACG89C_000925"
     CDS             25325..26665
                     /locus_tag="ACG89C_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4899500.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26814..27800)
                     /locus_tag="ACG89C_000926"
     CDS             complement(26814..27800)
                     /locus_tag="ACG89C_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS4899501.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8161 a         5266 c         5062 g         9459 t
ORIGIN      
        1 aaaatcgtca tttttccatc aaaatctgct tatgcaaaat tataaaaaaa ttttgcagtt
       61 tttcttactt tttttgtcgt ttaattgaat gataaaaacc agtttcttta ttcagttaat
      121 caaatatgaa cgaattagat atgaatcatt gtatttgccg ttgcggaagg attttttaat
      181 taaccacaga taaacattga taacgattcg cttattgtaa tcaacagatt aaatttcata
      241 tgttcatatg tacggataca ttttcggacc gattaatttt aaccattctg tttatttgct
      301 tacctattag atatcaataa aaaactgctt aacagctatt tcagctgtta agcagtttta
      361 ggatttttag ttcatattca ttgtttctta taatacagtg ttcaatactt ctcttaaagc
      421 acttgttgat ttgctgaaca ttgctttttc ttcttcgttg attgataatt caacaacttc
      481 acgaacacca ttttcgtcaa cgattgatgg cacacctgta aagatatctt tttcgtcgta
      541 ttcaccagtt aaataagcag atacaggcaa tacagcttgt tcgttattca agatagcttt
      601 tacgatacgt gcagtactca taccaatacc atagtaagtt gcttttttac gatcaatgat
      661 ctcataagca gcattacgga ctttatcagc aatcacatct aattcatctt gggcaatacg
      721 atggtctttt tctacaattt caaacactgg tttaccaccg acagttgtat gtgaccatgc
      781 agcaacttct gaatcaccat gttcacctaa gatatagccg tggacgctgc gtgggtcaac
      841 ttttaatttc aatgcgattt ctttacggaa acgagttgta tccaatgttg ttccagtacc
      901 gataacacgt gatgttggca gaccagattc gttccaagcg atatatgtca aaatatctac
      961 tgggtttgaa gcaacaacga taataccgtc gaatcctgat cccatgattt cttttacgat
     1021 ttggcgcatg attgacgcat ttgttttaac taaatctaaa cgagattgac caggtttttg
     1081 gttgatacca gctgtgataa caacgatatc agcatcttta cattcttcat agccacctga
     1141 ccatacagcc acattttcat cgccccaagc cataccatct aaaaggtcca atgcttcccc
     1201 ttctgctttt tcttgattca catcgatcaa tactaattca ttagagattc cttggttgat
     1261 cattgcataa gcgatacttg ttccaacaaa acctgtaccg acgattacta ctttacgact
     1321 tgtttttttc atgaataaac agctcctttt cataactatt tgttactttc atgaaaagta
     1381 tagtcttgtt tattcatttt atcaacatgt taattataat agttgttatt ataatttttg
     1441 atggaaacga attcctgaat acgcataaca gataaatgac aaatgtttac acctatatcc
     1501 cctgataata caaccaaaag tttagaactg aaaataaatc caaaaaaatt cgctgcaacg
     1561 atataataag acagcaatcg cacctaaaac aattttcctt catatgcttt agtgaaagat
     1621 tttcttctta atgtttattt ctccattcat tgggagtaca tgttgaatac ttcttgaata
     1681 atttgaataa agaagtcgtt tgtttatatc cgatacgatt agcgatttct tccaacgaaa
     1741 aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt tttctttaga accagttctt
     1801 gaaaggaagc atcaaaatac attttcaatt tattactaag atagaccgga ttgtagccaa
     1861 aatgagctgc tgtttctttt aacgtacaag aagtaaaatg cttatcgata taatctaata
     1921 ctctaagcat ctcattttct gcgcatccat ctgctgatga aaagctttgt tgttctaata
     1981 aatgagtcaa ttcgatcagt aatagtgatg tatagagatt taacgaacgg atcttatgac
     2041 tgtatgtatc aaaataaatc aacagcatat tttcaatcaa atgcgtcaaa cgacttgttt
     2101 ttccgccgcg aaaatgaatg aattgtgtat gttccccttt ttcttttgat gcatttatca
     2161 aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt caatatctct gtagataagg
     2221 tttcttcttt taataagatg ttgattaaaa tatcctcttg ccctgtgtat tcgattcttt
     2281 gagtagcttc tttatcaatg atcaccacgt cactttcttc catctctatc ctttctccat
     2341 taataatttg taaacatgta ccgctataca tataattgat ttcaacaaaa tcatgtgtat
     2401 gctcgggaac ttccgcatat cgacggtgtt tactgatatg aatatttccc tgttgaaata
     2461 gatcccctcg gtggattttt ggcggtgtct gaagatggta agctaagttt aaatcattga
     2521 tattcattcc ttgttcacgc tgcagagttt cgattgattc ttcgttacgc aaaaaaaatg
     2581 tctaattcag ctagttccaa ttgactccct cctttttgta agcggtttct ttcgcttatt
     2641 agttcacgct gtactgctat tatatgcaaa gctatttttg gtttcataaa gactttctat
     2701 ggttgggaca tgaacttcct gtgattccgc tattgagaga ataaccactt catactatat
     2761 ttttgataag cgcttacaaa aatgaaagga acatgcttat gaataaaaag atgatgaaat
     2821 tttctctgct gtcagtctct cttattttga cttctgctgg tgcagtcacg tctgcaattc
     2881 ccagtatgtc agctacttat gccacgcgtt ctttatctgc tgttgaatta ttaacgactg
     2941 ctccctcttt tatggtcact atttttgttt tgattagtag ttttattgct aggaaaatcg
     3001 gacaaaaaca aactgtgatc atcggattag tgattgcatc gatttgtggt atcctccccc
     3061 tcttctcaca aagttacagt attgtcttag cttcaagagc tggactaggt attggctttg
     3121 gattgatcaa ttctttggca gtcagcatga ttgctgcctt ttatcacgga gatgaacgag
     3181 cctctatgat tggttttcaa agtgcatttc aaggatttgg tgcagcttta atgacatttg
     3241 ttgcaggaca gctagtacag ttcggttggc aatatacctt tttagtctac gccatcagct
     3301 tacctattct tgtcttgttt gttttgtttg ttcctacacc tgaaacaact gaacttcaat
     3361 cgcatggcac agccccaaca agacaagtaa tcaaaacgaa tcctaaaatg attagttatg
     3421 gtatttttct cttgctggtc atcgtggtat ataatgctgt atcgattaaa ttagccacag
     3481 tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc gaatatctta acaatcatgc
     3541 aattggcaag tatggctgcc ggagtttgtt ttggctggct tcagacaaaa gcacatcgct
     3601 atatgcttac actttcttta ctactgatgg gaatcggctt tgtattgatt gccactgtga
     3661 taaacctgta tgtgattggg tttggtgcga tcctaacagg aatttctttt tcgttattca
     3721 ttccttactt attcaaccaa gtaagtatcc aagcgcccaa gtacgcggag agtttcaata
     3781 cttcaatctt attggttggc gctaatttag gtactttcat tgcaccatat ggtttgctct
     3841 ttctctcttt tgcttctctt gggagtaaaa cacttgctgt tttcgtcaat ggcagtatct
     3901 tgctgttgat tagcgcgctt attagccttc tagtagtaca caggacgaat agaaagacag
     3961 tgataagagt agatgaagaa ataaaagaac aattgacaac aaaataaatt tgatggaggt
     4021 cgtgtaaaat gacaaatgaa aaacataaag atagatggct ttggtatcct ggagactttg
     4081 agatccgcca tggactgcta caaaatttcc aacgggaaga acgaggtttt gactggccag
     4141 cgtactggta catggatgat tgccatcgga acgtcaagtt taagagatat tactttcttg
     4201 atcaaccttc catgtttaaa gtgacgatcc aaggagttgg gtatgtagaa attaatggac
     4261 aaaaacatcc atgtggaaaa tggctgactt gtccagctgg taaagcaaaa attcgtatct
     4321 ttgtaggaca tacatcaggg ctaccagcga tgtttatcga gggagacgaa gtaaaatcag
     4381 atattggctg gacagcaagt aattttattg aagaatatcc agctggctgg agtccgcttt
     4441 atgtcgatat cgcaaaagat ccaaatcaaa tctattatca aaaagaatgc gtccatccag
     4501 tcactgaaac ggagattggc ggaggaatct tattcgattt tgggcgagca gtgaatggct
     4561 gtgtagaact aatatctctg gatgaccata tcgaatctat cactctatgt tatggagagt
     4621 ccgatgtaga agctatggat atcgattatt gctactataa gcaaatagga atgacaagaa
     4681 aaatgaaagc tcgtaaacgc gcttttcgct atttgtttat accagaggtt tctactggaa
     4741 tggttcaact tcaagttttt catgaatttt tatctctttc gaaacaagct gctttttcaa
     4801 gcgattcttc tttactgaat cagatctggt cagtatcaga agaagccttc aagttatgta
     4861 gtgatttgtt ttttatcgac ggtatcaaac gagatcgctg gatttggtct ggagatgcgt
     4921 atcaatcatt catgatcaat caatatcttt tttttgacga ggaaatcaat aaacgaacga
     4981 tattagcatt aagaggacaa aatgaaatca agcagcatat gaatactatc gttgactatt
     5041 cgatcttgtg gttgattggt atcgaaaacc actatatgat gacgaaagac tatgaatttt
     5101 tacgcatcgt ctaccctaag atgcaatcat taatgagata cttgatggag caaacgaatg
     5161 aattagggtt tatttatggg agagcaaaag attggatatt tattgattgg tctgaaatgg
     5221 ataaagaagg aacgcttgct gcagaacaag tcttattact gaaagcctat cattccatga
     5281 tcacttgcgg agatatctta gctgaaacat atggagagaa cgaaaattat catactcatc
     5341 tctatcaaga aaaatatcaa ctattgaaac aaaacctatt tgactacttt tgggatgaag
     5401 aacttggcgc ttttatcgat tgctatgaat ctgggagaag aaatgtgacg cgtcatgcga
     5461 atattttcgc tgttttattc gattttgcta acaaagacca acagcaatcg atcttaaaga
     5521 atgtcttatt gaatgacact atcacacaga ttactactcc atatttcaaa ttctatgaac
     5581 aagatgcttt atgcaaactc ggacagacga atatcgtcta ccaaacgatt cttgattatt
     5641 ggggagggat gttatctcat gatgcggtaa ctttctggga agaatacgac cctactcaaa
     5701 caggaaatga aaagtacagt atgtatggag atccttatgg aaaaagctta tgccatgcat
     5761 ggggagcaag cccaatctat cttttaggac gctattttat cggtttgtat ccaacaaagc
     5821 caggttatga agaatttatg atacaaccta atctagattc atttgaaaca ctgaattgta
     5881 cgctacctat caaagatgga aagatttcgc ttgaaaaaac aaaagataag atcgtgatta
     5941 cttctgatag aacaggcgga acagtgaaaa tgaatggtaa aagttatcct cttctagcaa
     6001 aacagccact tgtcttaaca agctattcaa aaataaatgc ataaaacaaa aaactgcttg
     6061 cgacatatca gaagacttta aatcttcttc tgatattgtc caagcaattt ttattttgaa
     6121 gtttgcttaa ttctgttctt tagaaacaat gtttgttctt tttccggcaa aaagaaagct
     6181 agataatacg acaagtaaaa agaaagcgat gattccaact gtcaaggcta tttgaccgta
     6241 ttctcctagt ttcccaagta caagtcctgg tactagataa tcagtatcgg caaatgtaga
     6301 agaagcgatt ttaagatctc ctaatactgg aaggataaat aatggcagcc aactaatcaa
     6361 taagctattg aaagcagaac ctaataccgc ccctcttcta ccgccagtcg agttgccgaa
     6421 gacaccagaa gcagctccgc agaagaaatg tcctactacc ccaggaatga taactgtagt
     6481 ttgcaagcct atcatagcaa acatggatag tgttcctaca aagaagctaa caaaaaagcc
     6541 aattaataca gcattaggtg catatggaaa aacgatagga acatctaatg ctggtttaga
     6601 atcgggtacg agttttttag caatcccttg gaatgctgga acgatttctg ccaacaccat
     6661 tcgaacccct tgaaggacga taacaaaccc agctgtgaat gttccagctt ggatcagtgc
     6721 aaagattaca gcgttgtctc cattgcttag ttctctttca acaaatccag gaccagctaa
     6781 caaagcaagt gcgacatata cgatcgacat caagagcatg atcgatacag tagaatctcg
     6841 caaaaagctg aaacttttag gaatctttat gtcctctgta gaacggtctt tgtttccaaa
     6901 aaactttccg atccatccac tagaagcata accgatattt ccggtatgcc ctaacgcaac
     6961 ttgattgttt ccagtgatgt tcttcataaa cggttgacaa agagcaggcg taatagttaa
     7021 tagtgttcct tcgaagaccc cgcctaataa gatcatccac aaatttgcag taaaccccgc
     7081 gcttactaaa atgactgctg ttagacaaga aacataaagc attgcttgac ctgtaagaaa
     7141 aatgaattta tatcttgtga atctagcaag taaaatattc acgatcatcc ctactagcat
     7201 gatcaaagca gtaggtgtac cgtattgttg caaagcaatc gccactactg cttcattgct
     7261 aggaactact ccttgagtat gcagagctgt ttggaacatc gaagcaaaag gggttagtga
     7321 attactgatt aaaccagcac cgccgatcag tactaggaat ccgacaaatg ttttgatagt
     7381 tccttggatc acttctgaaa atttcttctt ctgaagaacg agaccaagca gtgcaatcaa
     7441 agaaacgagg atggagggct gactgacgat tgacacaaac aaattcaaca atttatccat
     7501 tgatagttcc tcccttgaat catttttatt cttttgacaa gatcttcgtt actttttctt
     7561 ttacttcttc gctgtcaatg atcgattgca aaggtacgag cttttcatca ggtatagaag
     7621 ttagcgtatc tttcaaagaa tgctcaatga agaaataatc agccatatcg ctagatacac
     7681 ttccaacatc cgtatgactt acttcaaact cgtccacatc tgctccaaga tctgcaagtg
     7741 cttgtttgat gttcatctct accataaaac ttgttcctaa tccactttga catactgcaa
     7801 aaattttcat atcattcgtc tccttttctt atgatcgcta atatttcttc ttttgttgat
     7861 gcgtgtaata aatcttgaag attttgttta ttagaaagta gctttgttaa atttgccaaa
     7921 gcccgaagat gtgcctcatt atcgatagca gctaaacaga tgaacaaact aatgggatgg
     7981 gctggattat caagcagatc cacaggttgt tgaagtttca gcaaagacat ccctacttcg
     8041 tttacgccat cttctggacg agcatgaggt aatgcgatgt gatcgccaat atggataaac
     8101 gcaccatact gctccacgcg ctcgatcata gctttgatat aactttcttc tattttattt
     8161 tctgataata gcggctgtgc ggcaaatgtg attgcttctt gccagttttt tggttgatcc
     8221 gataattgga tcatttcttc tgttaataat tcagatagca taggacgttg atcctccttc
     8281 ttttctaaaa gtttgttcat tttacgattt aatacttgat agagcttttc ttcgtctacg
     8341 ccttctttta attcgatata aggcagtagc gccgacaaca attcttgggc agaaggcatt
     8401 gaaaatcctg gtatcagcca atctctttgt acttgattga tcagctgatt tttttccaag
     8461 tcagtcatga ttggatgcag aacatagaga gactttttct tatcagtaag tgtgagaggt
     8521 acagtagaaa aaataatatc gtaagaagtt tcgtcaacct cgtgtaagcg gtcagctgaa
     8581 ttagtctctg taaaaaagat catcgggaat aattgctgaa gttcactttt taagatcaat
     8641 gaagagctaa tgccacttgg acagacgatc acggctctta tttttctatt tctttcttct
     8701 gcgtccgctt ttctgatctc tccgccaaat agtatagtga aaaaaccaat ctcttcttca
     8761 gggatgctct tccctattcg tctttctaac ggaagtaatg cttttcttgt catttcgtag
     8821 atggaagcat attgatgctt aatttgatcg atcatcacat taggtaaata aaaagaatag
     8881 gaaatacgaa aataggcagg gaccaagtga taaaagacat tcatcaataa ttcacgaaac
     8941 gaagcaaatt ctacggccat caggaattcc atgcgatgga tgacttctga agaacattcc
     9001 agcaaaaaat caagtgcagg ctcctggaca tctccttcaa cgatcgatgc taagcaaaga
     9061 gagaaaaata taatctccga attttgatgt tcggaatagc caaggccagt caatacttgt
     9121 cgactaatgt cagtgactgc aagagaagcc aacagttcag catcttttac cagttccatg
     9181 gatgtttttc caattcgaat ggttgtcaaa ctcaaataat aagctaattc atccattcga
     9241 tttggtgcga atcgaagatc tgatagtgaa atcgttttat ctagcaaatc tctaactttg
     9301 gcgtaataat caatggaaag ggttgcggtt aaatacgtga gcccaaatct tccagcaagt
     9361 gactgcatca aacgagcagt gcgatttttt gcttctcttc ttaatattgc ttctgaccct
     9421 agcagcctaa aaccagtttt cctagtatac gacagctgac aatcttccac ttctaattca
     9481 tttcttatct ttttgatatc tgacaggact gtccctttag aaacctgaag aaaatcttga
     9541 aaatgaaaaa cagaaacaga cgttgcagtc gaataaacca tcaaataaag aagatctctt
     9601 cgctcttgtt cggtatacac agcttcaaat cgagtaatat ccaaaaattt ttctgcccag
     9661 tcgattgctc ggaatttctt tatacggata cgggtatcat ccacatattc ggcaaaagga
     9721 gttaattgtt tgatcgcctc gcttgtattt acttcgatga tctctgctgc tttttctaga
     9781 ctgatttctc ttggtgtttt caggagataa ctaattttgt agttcacagc gctgttcacc
     9841 tcctacttat attctagtat ggtagcgctt ttaaatcgag gggtgtttgg tacaacttac
     9901 agttggattt tttttacgac tgtaccgaac gatttctatc gaatggatac agaaaaaaag
     9961 caggtgacca aatgtctcct gcttttatga attgatttcg tccgatcaat gatgatcatg
    10021 tttatttttt gaggtcagtt cttcaaattt ttctttgaca tcttcaaccg ctttttcgat
    10081 atctttttca aagtgattgg gctcattagg ctttgtttcc tcttctacag aatttgaacc
    10141 agataaagct gtttctttat tttctgtttc accagagttt gtgccagcta atgattcttc
    10201 tttgtcttct gtttcaccag aattagttcc tgctaaagcc acttcttctg ttttaggggc
    10261 cattgaacct cttaatcgtt tgtgtttttc accgtcataa tttgtcatgt tggttcctcc
    10321 tagtagttct atactttttc atcgtacaat aagacagttt atgcgtcaaa aaacaaaccc
    10381 gattcttaca aatatgtcat atttttcctt ctgattcttg aatgcttttg attccttctt
    10441 ccaaaatttt catttgtaag attgtttctt catctttgat gactttttct gcgccattaa
    10501 ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc accaacctcg gaaattactt
    10561 tttcttcatg ataactccct tgttcatcta tataggtcaa tacaccataa tgttcgggca
    10621 aatcgatacc aaagtcttca tgatcaggca tgtaaaacat tttcagatgt tcttcttgtc
    10681 gatcttttgt ttgtttcacg aacatccctt tttttccgta aacgacgaaa cttggacgtt
    10741 ctttgatgcg gaaataactg gattttatcg aaattttcat atttccatag aaaaaatcca
    10801 gatcgaagta atcattcatt cgtcctttcc ctaaaagttg acgtacatca tagctgatat
    10861 catctggttc gccgaaataa gaaagaactt gatctactgt atgacaagca tgtccatata
    10921 aatagctgtt cgcagtagaa aattcatgcg tattttctgg tacttccggt ctgaaataat
    10981 cataatgcat ttctacttct aaaatatccc ctaattttcc gctttcgatt actttttgga
    11041 ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca ttggataaat agaccttttt
    11101 cttttgctaa tgcaaataac tcttctgctt cctttgacgt ttctgcaaaa ggtttctcta
    11161 ctagtacatt cttaccatgt tcgagtgcca tttttccata ttcataatga aattgactag
    11221 gtgtagtgat gacaactaat tggatctcag gatcatccca aagatcagac agctgatcag
    11281 tatattcgat gtcttcgaat gttttccaca ctggttcaga tggggaaaaa atttttttga
    11341 tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg atagcgattt gtactctttc
    11401 cattgccaag ataagcgatt gtaagcatga gtggtgcctc cttttattga tgttttcagt
    11461 atatcctaga aaagcgcaga aagtatttat aatagctatt ctggtaaaaa agttcagaaa
    11521 aacacttgtt atcattcggt ttggtgaaat taatgttcaa agtaaggata aaaatcaaaa
    11581 agatagtagc ggaaccaagt gtttatcagt aagatagaat ttggaggtca gatcatgctt
    11641 attgaagaaa aactaacaaa acaagagttg tttaccacga cagagaaacg gattgtcgat
    11701 tacatccgta ggaatatcga agctgcagtc tacatgacga ttgaagaatt agcaaaagct
    11761 acgtatactt cccattcagc tatcattcgt ttatgcaaaa aaatgggatt cagcggattt
    11821 aaagaattcc gtttcgagct ggggcgggaa gttcatcaat tgattgctca gttcaatcaa
    11881 acgattgatc ctaattttcc tttttcaagt gaagatagca ctcagcaaat tgcaaagaaa
    11941 atggcggatt tatcgatcca gtcaataaaa aaagcacaat tgcagattga acatgaagat
    12001 ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa tctttctttt tgcaaaaggt
    12061 gattctcaaa tcacggcaag gaaattccag aataaaatgg tcaagttgaa taagtttttg
    12121 atcatggctg aagaatacag tgattcttct tggaatgcag ccaatttgac tagcgaggat
    12181 tgcgccattt ttattagcta cagcggtcgc atccatcatt atgaacgaat aatgacctat
    12241 ttgaagcatg tagaggcccc taccttgctg attaccggaa atcagcattc agaaatggcc
    12301 aaacaggctg atatgtgtct tgttatatcg caggaggaat atgattttgc taaagtagcg
    12361 actttctcct ctcaaattgc cttcgattac gtattaaata cgttattctc agtgatatat
    12421 tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca aacaagaatt gatgcaaaaa
    12481 ggccttctta aagaatatcg gaaaacttaa gaaaagatcc tcatttagta ataagaaaga
    12541 tagtgtttga taggctctct ttgatatcat cataaaaaca ggctgaagtt ttctttttca
    12601 aacctcagcc tgtttgttta cggaacgatt cgaatagata tattatagaa atgaggaata
    12661 gctaacttct gtttcttatt ttgaacgatt gacgaaaaag atatataaga aggaggaaaa
    12721 tcagtataca tgcagctaac aatccataca ggaaaaatcg tttgttctcg gatactttct
    12781 gtacttcttt ttgaacttct tttgcatgat aaggaacccg gtgccctctg acaagtaagc
    12841 gatgactgtt gaccatataa ggtgtacaag ttaggagtgt cacgaaatcc tgcccttttt
    12901 cgattctcag atcttttgta tcagtcggtt ctaccgtctt gatctgatcg acttgataag
    12961 cgagtgtccg tccattgatt tcgatataga aagaatcacc taaagtcagt ttcggtaagt
    13021 ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga aataacggca tgtgtatttt
    13081 ctccccccgt aggataagac gttccttcca ataatgaggc acctttttct agcagcacag
    13141 tattcgtttc atcaaaaatt ggcaaatgga cattgatctc agggatcgtc aaaataccga
    13201 tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg ttcttttttc ttagtaaagg
    13261 gatctgcacc tggatttcca ccttcttttg ctaattgctg gtttttcttt gtcatgtttt
    13321 cttggatttt agccatgact tcttcatttt ctttgaccgc ttgttgctga taatgagaga
    13381 tgatctgctg atccagataa ttattcagcg cgtcgctaac aaatggataa gctagtatcc
    13441 ctattcctga gagaagcaag aaaagcatca gcagatctag aagtttcctt ttctttggtt
    13501 tcatttgtgt tctccttgtg atgactgttt gataataaaa gaaaaaggag catttgcgta
    13561 atccgctgct cctttttcca attcttgcaa aaagactaaa tctgactgtg cttgcgtcta
    13621 gcaaagtaca gtccagcaat cagtagaagg actactcctg caccgatata cacatagatt
    13681 cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat ttggtatttt ttcaggagaa
    13741 atcaactgtc ctgctgttac ataagattgt tcatcgatag taaatgctac acggtttgtt
    13801 aatggaacat atttttctgg cgctttcgtt tcttccagat agtacgtgcc atattttaga
    13861 cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa ccgttgctga ttctttcgcc
    13921 gatacccagc tgacggcttt tgtggatggg tcgatcgata aatatttcgc tgtgtcacta
    13981 tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt ggtctgatgt aacgtcacca
    14041 tctactttaa caaatcgttt gcccccagta acgacatcga ctgacggtgg tgtttgatca
    14101 tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc ctttggttgg atctgctttt
    14161 tcgttcaaat gcatatagta aacgaatttc aatgtaccgc caggagttaa tgaaggaata
    14221 taattcggat caactgaaac cgtgaatccg tctgtttgct cagtgacaga ataatttact
    14281 gggtcgattt ctttatttcc atcatataag gcataagcat acgttcctga agaatcatta
    14341 tcaaatgtta aagcagcgtc atgagtatcg ataagtttga atgttgtgta cttgttttgc
    14401 gttccttctt tatcagcgat acctaatggg atattgacag aaatctcata ttgaattttt
    14461 tcaccgattg cgacatcttt tccattcaat tgaggtgttg ttttatcaac tttagacgta
    14521 tcatttttga tggtcttttc tactggtgtt tggctatttg ccaggatctc aaaaggcgtg
    14581 attgtttgct tttcaatcat ttccgcatta tcaggagctt ttacttcttc taaattatat
    14641 tttccaactt ctaaatgatt aacgatcaac tggccttttt caatagatgc ctcggcaaag
    14701 tcattgttgc cgatgtcata agaatgacca gtaatgaaat gtttggcttt ggtttgatca
    14761 gttgtccaag tgtacaatcc atctgtgaca ctttggatgt attttttgac gcttggtgtt
    14821 ccttcttctt tagaaataat aaattctgct ccatttagtg cttcgttttc ggcagtaccg
    14881 atttttgtaa ctttcaacgt tccatcatta ccgactgtat ttttagggta gagatggata
    14941 gtatctagtt cttctgtccc gtatttataa gagccatctg cttgtttgat catctcatat
    15001 acagggaaag ctaaaaccat gtttgcggca gctgacactc cgtcttttgg ttcttctttg
    15061 atcgtgtaga ctgcatcttt cccattttgt tttttaggta aagataaagt gacatttcca
    15121 tcagcatctg tcgttcctga agcaactggt gtaccaggag tcaaagactg gactgcttgt
    15181 tttgctgcat ccacggacgc tcctttatct cgttgcgcat aaaattcttg agtgacgtta
    15241 taaactgaaa atgaaatatc ggctaatcct tggtattgat cgaattcgct catttctttc
    15301 ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt tctttttatg aaggatgact
    15361 tgagctgctg tctcctctgc aaacactttt tttgcgccga agctgtttgt gagtaatggt
    15421 aaaataagga aaaggactcc taacataacg tttatttttt tatgattttt cattttttca
    15481 ctcctgttct ttttttgata aacaaccaga ttgcaaagct tgcgaatacc ataccaacaa
    15541 cgcctaaaag tgtatttttc gtttcgtttg tttttggaag aaatggacgg ctattttctt
    15601 ttttattgac tactttcaat atactgtccg cctctttatc ctcagaaaga aaagttgaaa
    15661 tggtaaacgg aatttctgtt tcacttaaaa tataaccaga aggtgcttcg atctctttta
    15721 atcgataact tcctgttttc aacccggaaa ttgaaaaagc gccattttta tcggaaatca
    15781 ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc gttggctgtt tgctggaggt
    15841 aatttcctgc tttgttttta ataaggaata ctgcgccagc taatcgttta tctggtttat
    15901 ttgtctcgat tttgacaaat tgcttactcc ctgtatatac agagactttc tttgtactga
    15961 caccaaaacc aaaatccagt gttccgttgt tgttgatttc cttgttcgcc tgtgctgtac
    16021 tgcttagctg catctgataa gaaatggtca gtttttttcc agcatatttt tccaacgaag
    16081 ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg tatgacaaag ccttctgtca
    16141 gcttttctcc attcgatgaa atcgttaaac tattaggtaa aaacgttaat gcttcatccg
    16201 cactatctga caattcgaac ttttgatagt caaggatatt tacaggaatt gtcgtatgca
    16261 aggaataagt gattttttca ccaatcgtaa aatcatttct aggctcttct aataccttct
    16321 caaaaggtgg atcagggtag tcgttttctt catttttagg atacaaatgg atagttgaaa
    16381 gtttttgatt gttttgtcca tgtacaggaa gaacaactac catattctct gccttttctt
    16441 tgacgacttc tggtactttg gattcaatga aaagatacgc tttatctctt ttttcttgat
    16501 ctttagcggc cagagaaaaa gaagcgatac catcttcgtt gttaagagtt gtagttgttg
    16561 cttctgcggt aaacataccg gaagacgcac cgttttttgc gatctctgct tgtgcttctt
    16621 ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc tgtgacatca taaacttgga
    16681 atgtgacacc atttaatcct cgatacgttt gtaataaagc tttttcttgt ccgtcatttg
    16741 gatgattttt cggcagttgc ccgtttggga aaagcaattt gtgtaaagta atctgaacca
    16801 tttctgtttc tgtttctgtt gctacctgag taaaagctgg tttaaatagt aacaagaaga
    16861 gacacatact aagccaacca agttttttca tatttagctg ccccctcttt cttttttcaa
    16921 aaaaagatac caaatagaaa acgcacaacc aatcatccct actagataga ttcctaaacg
    16981 gccaattcct cccgtttcag gtaatggtac ttttgcctga ttcgtgatgt ctaaagaaat
    17041 ctggttgttt ttggctcctg gtgacagaac agattcatga tcttgtccat ccacttgaat
    17101 tgacccatct tcgtgtataa ctatagtaac tggctctttt agaccttggt atccttctgg
    17161 tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa ttttcgaata gaaaggtatc
    17221 tgtttcttgt ccatcttttg gcgattctaa gtccatttct ggtccctgca gtctgaattt
    17281 tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt accgttagat caaacgcttt
    17341 tagtcgattt tgatgagtga gcgtccatac attatttgtc atctcggtat tttttcccgt
    17401 gtaaatgatt tttccatatt tatcgatttg gaattcataa tttcccggcg aagtatcata
    17461 tccggcagga ccagctgttt caacgactcg atatttacca ggtgtgagtg aatcaaaact
    17521 ggcaagacct ttttcattcg ttgtttggct gtcaattggc tgataagtac catttgcttc
    17581 ttttttctgt agggaaaatg ctgctccttt aagatttatc tctttgtccg tcccttgcat
    17641 cgcatactta cgtactgcta caggaacttc aacaaaagga ttttcaatag tcaactggat
    17701 cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt accgtaccat cagaagcgat
    17761 cttgatttcc caagtagtct ttttgcttaa tccatgccct tctggagctt ttgtttctgt
    17821 cagcgtatag gtcatttctt tttgcaattt gacattttct ggaagagaat aggttccgtc
    17881 gccatgatct gttagtaaag tatcaataga atctcctgtt aatttgaaaa cagcgccaat
    17941 aagatccttt tcacctgtag aggaattttt cgttattttc aagtttaacg gtgtgaattg
    18001 tttagtattt ttccatgtca ttgcatctat ttgttgagaa tcgtatcctg gtacaggtaa
    18061 ttctttgatt gtttggtaac tgaatgcttg accttgatta ttgtattgag gtagtgataa
    18121 tatctcttga taactttctc cgctatttgc agataatttg tcaatgtctg cacgttccca
    18181 cgtatttgtt gtatctttag ctggtttaat gattcgaata tatccgtttt tccaagctgc
    18241 agcatttgtc gtatgttccc gttgaatctc aaaagtaact tgatctggac gatcagctag
    18301 attgttgtca aattcttccc ataacttttt gatgtgaaga ctgactccgg gagctttagc
    18361 agatggtata ccgaattcag ctaattcatt ggtatcaatg tttggctgga aagttgtcct
    18421 gccgttcatt tgataccaga aatttggatg gaagtcctca ttttctgttt ggattctcac
    18481 ttggtaatgg atttggattt cttggtcttt tcctaaatag atctgattgc tcttgatggt
    18541 atttccttct atggaaatag atggagtgac cgttgtagga cttgtcccca cactcttgac
    18601 tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt ggatcattta tttgtccatt
    18661 gcttacagtt gctgagatct gtacagcttt tttggctagg tattcagaga tatctgttgc
    18721 atgatcagct gattcatagt aaagatcgcc tttttcatct gatgaaacca tttgtcgcat
    18781 acgagactct acttctgctt ttgagagacc agctgccgga tcgctttgaa gttggatgcc
    18841 aagaccatgt atttcgattc ctcgttcttt gagtgccatc gcttctccga tggttgcgat
    18901 aaacgtactg tcgattcttc tggatagatt gttttggtca ggtgcatcat agattcttga
    18961 gattagagaa gtatttcccg gccgatcttg cgtattagaa aactgagttc cgtaataatt
    19021 gctgcttgat tgtgcgtgta cccgctgtac tttataggaa aatgttggta caccatccgt
    19081 cagcaaaacg atcacttttt tatgtccatt tggaacggat agcatgcttc ctgcatctct
    19141 tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa ggtgtaatgg attttacttg
    19201 atttttcact gaatcaaatg aacccatctg tactgcaccg ttactgtagc tatatccttc
    19261 gcttgagtag ccgacatatc ccatattgat tttgtctgtg ataccgctat ctgctaaagt
    19321 atcgacaaaa cgatcgacac caatctttac ttcaccgatc cgattattgt cgttcatact
    19381 tcctgaccag tctacgacca atacgagatc aagaggcgtg atatcttttt gtacatttcc
    19441 acgagcattc aaataaacat caaacaatcc gggtgtactt gtttcctttg catactttcg
    19501 tatcgcatag tctgcttgat taccggttcc tccatattcg atatacgaat gggtccgatc
    19561 attaggatct ccattccaag aatttatacc atcccattga tttgttccgt ttttattgcc
    19621 ttgatgatca agaacatttg tatttcctgt aggttgccaa ctggctttag gataagtgcc
    19681 ctgatcatct gttgtatatt ctgtaggaat cgtttgatta gatggcacac tcacagctgg
    19741 caaggataat gtaggcaatg taaatgggct gacagaattt tcgttttctt ttgatgattc
    19801 ttcttctatt tcgtctggct ttggtactga cacatctgta tctttcttat tttctgtctc
    19861 atcaataatg tcatattctt tattatcgac gatcagcttg tttgcctggc tgtggtaaag
    19921 tgctggatcg attggaaaac tcagatcgat agtttgttta gtgtttgatg agatttccaa
    19981 cgtaattttc ccgctattta cctgataagc atagccatct ccttcttttc tttccacatc
    20041 acttaagtaa ttttcgacta gaccaatttc tttttctatc ttttcagcac tggtgttatt
    20101 cgcatttagc ttgatgttca gtgctttttt tccagactga tccgaaatga cttcagctga
    20161 aattgtttct aaaactgttg gatcatctgt agtgtctgct attgcagata atggcacaaa
    20221 gtttgataaa ctcaatatta tcagcatgaa aataactttc agtttcttcc ctgtggtcgt
    20281 catttttccc ctcctttttc tttttttctg ttttgacgtg aatagtgaaa attattaaaa
    20341 taaacaggcg ttacataatt ttatttattt taatatgata attattatac gaagggcgct
    20401 aaagggaatc acaccaattt tcataccaat tggtttcatt tatgaagttg attccttcga
    20461 ttaatgagcg tttcattgaa agaaaagggt tgagggagaa aaacatatga tcaggttgtt
    20521 ggaaggaaac tatagaaaga tggaaaaaat cattcgaatt ctgtccattt ctgacaagtg
    20581 gatgactaaa aaagaacttg ccaaacgaat tggtagttca gaatctacat ttgtaaggta
    20641 tatcgaagag atcaaaatcc gctggggaac ggatgtaacg attcaatctt cacaaaaatt
    20701 ggggtatcgt cttgaacatt tgaatgtacc agcttattta aatgttctga tggaaatgtc
    20761 gcaaagttct acaaacagtc gtttattaaa tgagattatt gtcaatccgg gtaaaacgat
    20821 tgagtattat tgtgaaaaaa tttccatcag ccggtcttct tttacacgta agttgaaaca
    20881 atgcaatcaa attttagaaa gctatttatt aaaagtgatc gttgaccaag gttttcagtt
    20941 aactagcctt aaaagtgagc tccagttgcg tatctttgtt acctttttct ttttaagtta
    21001 ctacggaaga cgcacgttgc catttccaat cgaaaaaaag aagatcaaag aaattatggt
    21061 caagaataat tgtcagatga atttgtttca ggaagaaagc ccatatgagc aatctttctt
    21121 tattatgtat tttgttgtgc atctcatgag agaatcccaa ggaactccat ttgaatggaa
    21181 agctggagac tcttatgtac agttgataaa tatcgataaa aaagacttct taatcttgaa
    21241 aaatctctta ggaaaattga agtacagcag ctatataaaa gtagtgaact attttgtcaa
    21301 tacgattttt tcagaatttt cggagaaaga aaaaaatgaa atcagtgagc agatacgaaa
    21361 aaatatgtcc tactgttcgt tttcatccgc tgattgcaaa gaaaaaaacg aagaatatgt
    21421 tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt ttcccatacg atacgactca
    21481 tttgacgatc cgtgcaactt actttgcaca gcagatgcaa caccgccaga tacgtctata
    21541 tgctggattt cgtgcagaac ttcaatttta ttccactcta ctgaatcaga atctgattta
    21601 ttatttatct agctatatct tttggatgct tatcggtaat cctgaaattc accattatac
    21661 ttctgataaa aaaatactga ttatcagcga cttaagtctt cgacatagtc aatatattga
    21721 agaatatatc agtgatattc ttgctgtgca taaaattcac tctgagacaa ctgctattac
    21781 tgaagatcag ctgtcgaaat acaatcttct cgagtatgat ctgatcgtta cgaatcaacc
    21841 cattttgaat gcccatgttc ctcatatatt gattgatgat agtgtgtcat tcgcaaatga
    21901 agaagaactc atccgcttat tcgagttatg aataatcaaa aaacagctat gtataaacca
    21961 ccctgataaa aatttcagga caaatacaca tagctgtttc tttttattgt attccttcag
    22021 caatccaacg agatttatag cgaaagtctt ttaacaactc ttcttttgtc caattatttg
    22081 caatgagatc cgtcaaagtg ttcattacga gggtcatgga aaagtatagg aaactaactg
    22141 cttcttccga cgattgaatg gagaaatgat tgattcttaa taattccagc catttttcgt
    22201 tcattggaga atcagccgat agttctctac gtttggttgt ctgctgatca tttcgctggg
    22261 ttaacaagta taacctttgc caatagatac tttcgtgatc taagtcgcag caccactcta
    22321 aatatttttc gatccccaaa aagaaatgtt gtttgttttc gctaatgaat tttaagatat
    22381 cttgatggac tatgtgagaa ctgcttttga tcgtatacaa ataagcatct tccaaatctt
    22441 gaaaatattt ataaaaagca ccgcgcgaca tccccatctt gtcaacgatt tctgagactt
    22501 tgacttggct gatgtgctgt gagcaaaata tctccagtaa taggtcatcg attctctgtt
    22561 tctttgcgtt cgataaattg aaaaaagttt cttttggcat ccacatctct cctttatctc
    22621 tgattatacg tggatagccc ttcattgtca tgaaggattc attccttctt tttctcttct
    22681 tctgtcaagt acccatcttc cattcgataa acgcgatcac tccattgaat catacggaca
    22741 tcatgagtaa ccataatagt cgctttttgt ttctcatgag cttctttaac tagtaatttg
    22801 acgacttcat atgcatgatc tgtatccaaa ctagctgttg gttcgtctgc taagatcaag
    22861 cttggctcat tatataaagc gcgagcaatt gccactctct gtctttctcc accagaaagg
    22921 tctttaggat aactattttt aagtgactgg atatctaaag attgcagcaa ggaatcgatt
    22981 tttgttgaat cttctttttt tgaagtaact tggtcaacta gttcaaattg ttcctttacc
    23041 gttaaaaaag gaatcaaatt agagctttgg agaataaatc ctatttcttt aaagcgtaaa
    23101 tctgctcgtt ttttttctgg aagctcagtt agtgagtgac cgttgatcac tatttctccg
    23161 gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag tacttttacc tgaaccagaa
    23221 ggaccaatga tgctgacgaa ttctccttcc tttacataaa aatcaattgc tttcaaagct
    23281 gagacctttg tatgaccaga accaaatgtt ttggtaactt ttttcatcgt taatattttt
    23341 ttcattttta ttaacctcct attgctgtaa ttgggtctac tttggttatc gtacgtatcg
    23401 aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc actatatagc aaccattgtg
    23461 accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag actagtcaaa taagcaaaca
    23521 aaagagcaag cagaacgcct accaccccaa caataaagga ctgtgctact aaagatttag
    23581 cgatgaagaa atttttgatc ccttgtgctt tcattactcc aaaaatagca gtcttctgaa
    23641 gtgtcatcac gtacatgaaa ataccgacca cggcagcaac cactaagaac aaaaagtata
    23701 tcatcgcatt taaggtcata ttttgggcag aatagccagg aatactttca atgaatgtcc
    23761 caatagttaa taactgtaga cccttcgatg ctttttcttt cgaaatttta gctgaatcct
    23821 ttattacgat cccattgatc ggctgttctt cttcagaagc aaacggctgt tgtccatact
    23881 tcaaagcagt ccacgtatct agggacgcat agatgacggg agaaactgtg taataagttt
    23941 caggaaagat accgacaatc gtcaaaggat cgtcgtaact gccaattttg atcgtatctc
    24001 caattttata gcctgaatca gccagatttt gagagatgat gatttcattc tttgttttaa
    24061 attcatgccc tttcgtgagt tttggtaata aaaatgcttc tttcgttgtg ccgaaaactg
    24121 taacattttc tttgtttttt cctttgattg ctccgctgta caatccgatt ggtgctttct
    24181 ctcctccttc aatgtatttc agatctcctc ttgttaattg tgaagctgca aatacttgat
    24241 ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc atcgattgct tttttaaact
    24301 cttctgaaag tccattcgct agtcctgaca acatgaatac gacatacgca atcaagaaca
    24361 taataccgac gatcaagcca tatcgtaatt tagaatagcg aatttctttg atagctagaa
    24421 acatttgttt ttcctcccta tgatagattg atagtgacaa aatgtcactt gttgaatagt
    24481 gtaactctaa agtgacaatt tgtcacttgt gttgtttttt tcttatcaat aaattgtcta
    24541 agacatcttt cgataaaacc ttaacatgag tcataaaaaa tttatttaag ccttatttat
    24601 gctttccatg ataaactaaa gtgaccaaat caatgaggtg ggaaaatgat taaactatta
    24661 gtagtagaag acgataaagt attatccaat aatataaaag aaatactcgc tgcaatcggc
    24721 gagataacac aagtttatga tggtagtgaa ggactgttcg agttggaaag tggagtttat
    24781 gatcttgcaa tcttggattt gatgatgccg gaaatgaatg gatatgaagt gttagcaaaa
    24841 atacggaaag aaaaaataca gactccagta ttgattttga ctgcaaaaga tagtttggaa
    24901 gataagattg aaggatttga acgaggtgca gatgattatc taacaaaacc tttctatcga
    24961 gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct cgctaggtct ctttgatgaa
    25021 aatgttctga agtataagga tatcagttgt gatttaaaaa gcaatgaagt cttgtataaa
    25081 gaacaagttt tgcccataca aggaaaagag tttgaattgc tcgtttattt tttacaaaat
    25141 aagggaatca ttttgacaaa agagcagata tttgaccgga tctggggatt tgattctgat
    25201 acgacgatta cagttattga agtttatatg agcaacttac gaaaacatct gcgaagtact
    25261 ggattggata aagaaatcaa aaccttacgt aatgtcggtt atatactgca aagggtgtga
    25321 tgcaatgaat ggagatatta cacgaaagca acgtagtcgc ttttttgcga caaatgtagc
    25381 agcatttgct tttatctttt ttcttttggg attcattact ttacagatat taaaccaatc
    25441 tgcatacaga gaaacagatc tgtcattaga gaaaataaat tttgattctc gaacagtaca
    25501 attagagata caaagatatc agcaaggcga tcccttttta aacccgactc aaacggaaca
    25561 aaacagaaca aacatggaac agccattaat ggaaccatca aatgaccgtt ttaacactca
    25621 agtgatcttg tggtctaaaa atggtgagat attgaataaa gcagctattg gtgggcgttt
    25681 caaccaaatc aaagaattaa aaatggatac gaaaaatctg aataccatcc aaaatattga
    25741 attaaatgaa gacagtggct catctgccct ctctttccgt tcgattacga aaaaagcgga
    25801 gacgaatcaa gatggcgttg cctacatcca aattttagaa aatacaaatc aaatcaagaa
    25861 ttcattagcg acttttcgca cgattatgat tttgtgtatg attgtttttt ggattctctc
    25921 tattggtatc agttattact tgtctagtct aagcatgcgc cctattattc tttcttggag
    25981 aagacaaaaa gaatttgttg agaatgcttc tcatgaattg cgcacaccgt taacgattat
    26041 tcaaaatagt ttggaacatc tttttaccaa gcctaatcat actattattg aagaatccga
    26101 aagcatcgct caagcattgt cagagacgag gcgcctcact ggattgactt cggatttgct
    26161 gacaatcgct cggaatgatt ctgatcagca gctgctttca aagcaaatga tcaatacaca
    26221 agaatatatc gaacaacttg ttaaaccatt tcaagagatg gccatcatag atggaaaaga
    26281 gtttattctc cataacgatg caacattaga agtaccaatt gatgaaaaaa aaatccatca
    26341 agttttggtt attttgctgg ataatgcatt aaaatatacg aatccagaag ataagatcat
    26401 tttggattca tctgttaacc ataaagaatg gctgattcac gtaaagaata ctggcccttc
    26461 tatcagtgaa gaagatcaag caaggatttt tgatcgattt tatcgggaag atcaatctcg
    26521 ggcgaaagag actggcggat acgggcttgg cttggcaatt gcaaaacagg taatcgaaca
    26581 gcatcatgga cagatttctg tatccaatta tcttccacaa ggagtcgatt ttaaagtcca
    26641 attgccgttg aagcctaaaa aataatgctt atccctaaaa aagggctgtg acaaaaatct
    26701 tgtcacagcc cttttttgtt gcgaataaac ggtgttccag aagtagcttc ttcggaaata
    26761 agccgaaatt tctttaagct gaccacttct gtcacaacct ctttagctta cgcttacgat
    26821 tttttattgc ggtcttcaat gtgtacatca ccgcttgtgt caatatccaa ttcttctcgg
    26881 ctaacatctt ctgtcacttt ttcaacatct tcatgttctt ctttatgaat cccaacttct
    26941 tcacgaataa ctgtatgttt gcttacatct acttgctctt ctttgattgg gatagtaatc
    27001 gtgtcatctt ccaatttttc gtctgttccc tgtgaagatt gatctgttac tggtttgcgt
    27061 tcgatgacga tttcttcacg tttgaccggt acttctaccg tttcagtatc gttgaccaca
    27121 tgtttgtgga tgtcgacttc tccagttgtc acatcatgtg tatttacatc taatttttct
    27181 tcttttagtt gaattgtatc atcatctaga tttggtttgc gatcgatgtc ttcagtcttg
    27241 cttgtaccca ttgtatcagt ctcgccaggt acgtccatcg gatcagttcc tggtgtcata
    27301 ttcaatggat ccgttgtatt ttgaaagcct gtcgtgccac ctacgtccat tggatctgtt
    27361 ccagtgccag tttctaatcc agtccccatt gtgcttgctg ggctaacagt atcttgtgta
    27421 tccattgtat cttctgttgt ttctggctga cgatagcctt ttactgtgat gacaaatttt
    27481 ccatcagaaa tatctcctct atattgatat aaaggatcat tttctggtgt agccgtctca
    27541 gaatcatagg tatcaaaaga aaaagtatct tttatttttt cccataaaga acggtcttct
    27601 tcaccatcca tccgattttc atctgtttca acatctatac cagacaatcc gtcaaatcct
    27661 agtctagatg ctgcttcatc agtagtataa ataacgatat catctcgttc atacccttcg
    27721 ttacgcaacc gttcaattac tgataatgct tcttccctcg tactatagct tccgatgacc
    27781 gttaaatttt tctcttccat gtaaattcct cctagcaaat agatttatga ttggtatatt
    27841 gttacttata aatttattct ataggagacg attgttttct acaaatattt agattggaat
    27901 ttgattaaaa caatttaatt gaaatttgaa aacatttatg ttaatgaa
//
LOCUS       DAZUKR010000024        26593 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000024 DAZUKR010000000
VERSION     DAZUKR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.024"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..492
                     /locus_tag="ACG89C_000927"
     CDS             55..492
                     /locus_tag="ACG89C_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899502.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            587..1471
                     /locus_tag="ACG89C_000928"
     CDS             587..1471
                     /locus_tag="ACG89C_000928"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4899503.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1695..4862
                     /locus_tag="ACG89C_000929"
     CDS             1695..4862
                     /locus_tag="ACG89C_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4899504.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4930..5427
                     /locus_tag="ACG89C_000930"
     CDS             4930..5427
                     /locus_tag="ACG89C_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS4899505.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5446..5910
                     /locus_tag="ACG89C_000931"
                     /pseudo
     CDS             5446..5910
                     /locus_tag="ACG89C_000931"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5907..6665
                     /locus_tag="ACG89C_000932"
     CDS             5907..6665
                     /locus_tag="ACG89C_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4899506.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6751..7491)
                     /locus_tag="ACG89C_000933"
     CDS             complement(6751..7491)
                     /locus_tag="ACG89C_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4899507.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7552..8805
                     /locus_tag="ACG89C_000934"
     CDS             7552..8805
                     /locus_tag="ACG89C_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4899508.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9127..9354
                     /locus_tag="ACG89C_000935"
     CDS             9127..9354
                     /locus_tag="ACG89C_000935"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4899509.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9477..9803
                     /locus_tag="ACG89C_000936"
     CDS             9477..9803
                     /locus_tag="ACG89C_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4899510.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9871..10584
                     /locus_tag="ACG89C_000937"
     CDS             9871..10584
                     /locus_tag="ACG89C_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4899511.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10894..11745
                     /gene="licT"
                     /locus_tag="ACG89C_000938"
     CDS             10894..11745
                     /gene="licT"
                     /locus_tag="ACG89C_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4899512.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11866..13731
                     /locus_tag="ACG89C_000939"
     CDS             11866..13731
                     /locus_tag="ACG89C_000939"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4899513.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13745..15188
                     /locus_tag="ACG89C_000940"
                     /pseudo
     CDS             13745..15188
                     /locus_tag="ACG89C_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15296..15997
                     /locus_tag="ACG89C_000941"
     CDS             15296..15997
                     /locus_tag="ACG89C_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4899514.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16123..16635
                     /locus_tag="ACG89C_000942"
                     /pseudo
     CDS             16123..16635
                     /locus_tag="ACG89C_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(17033..17242)
                     /locus_tag="ACG89C_000943"
     CDS             complement(17033..17242)
                     /locus_tag="ACG89C_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS4899515.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            17483..18058
                     /locus_tag="ACG89C_000944"
     CDS             17483..18058
                     /locus_tag="ACG89C_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS4899516.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18058..18498
                     /locus_tag="ACG89C_000945"
     CDS             18058..18498
                     /locus_tag="ACG89C_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4899517.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18514..19215
                     /locus_tag="ACG89C_000946"
     CDS             18514..19215
                     /locus_tag="ACG89C_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4899518.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(19703..20128)
                     /locus_tag="ACG89C_000947"
     CDS             complement(19703..20128)
                     /locus_tag="ACG89C_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899519.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            20502..21473
                     /locus_tag="ACG89C_000948"
     CDS             20502..21473
                     /locus_tag="ACG89C_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4899520.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            21505..22902
                     /locus_tag="ACG89C_000949"
     CDS             21505..22902
                     /locus_tag="ACG89C_000949"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4899521.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            23061..23684
                     /locus_tag="ACG89C_000950"
     CDS             23061..23684
                     /locus_tag="ACG89C_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899522.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            24433..26526
                     /locus_tag="ACG89C_000951"
     CDS             24433..26526
                     /locus_tag="ACG89C_000951"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4899523.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         8956 a         4432 c         5621 g         7584 t
ORIGIN      
        1 gtataatctt aaataaagaa aacaatatca aaaaatgcac gtagaagtag ataaatggag
       61 tggttttgca aatttctttt atttctagaa gttattcacg tatacggatt tgtttttctc
      121 acaaataaaa aggcaggaag tacaatggat catttgtata taaaatatat tcatcaattt
      181 tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat tattcaaggc
      241 cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat tcctctgatc
      301 gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt gctatttttg
      361 tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct atcattttct
      421 tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat gttttatctt
      481 gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat ttttttcttt
      541 atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga aaaaagtttt
      601 attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc aattagcgat
      661 agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa aagcagggga
      721 tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt ttgtcatggg
      781 cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac atcggccaaa
      841 tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag ggataccgct
      901 agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat tggatattgg
      961 aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc ctgaatcaat
     1021 caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt tcatgtcagg
     1081 aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg atggaaagaa
     1141 ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg gcggttttga
     1201 aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt atttaaaaga
     1261 ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag tagatgaatc
     1321 tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag ctgatgagga
     1381 agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga caatcaaagt
     1441 attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag gagttggcgg
     1501 gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca gccttttttt
     1561 tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata catattttaa
     1621 atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca aaacgtcaga
     1681 gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt ttgtttgtct
     1741 tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac gaatcaacgc
     1801 aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt acttctgaaa
     1861 ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat aagacaagtg
     1921 agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg gcaagagctg
     1981 tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag cttctcgcag
     2041 acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg gaattagaga
     2101 tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc acacaccata
     2161 tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga acagctgcac
     2221 atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct tatatgaccg
     2281 atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg acacttgatt
     2341 tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc acattagatg
     2401 gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc gctccgggta
     2461 aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa ctttatggac
     2521 cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt agtggaaaaa
     2581 cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat gatcgggcag
     2641 atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc atgtttgaag
     2701 tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc aatgacaaca
     2761 atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg aatggtggga
     2821 aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat tctagtcaaa
     2881 tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac gatcaacccg
     2941 ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa ggagccggat
     3001 cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt gagaatggta
     3061 cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca ggtgatggca
     3121 aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat gacgcttctg
     3181 gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa tcaaatatcg
     3241 atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat ttaaatttta
     3301 cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat aagaccggca
     3361 tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt gatttccaaa
     3421 acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa ggaaatggag
     3481 attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca ggcgcaacat
     3541 tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta tcgatcaatg
     3601 ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc
     3661 agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag ctcccgatga
     3721 ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc
     3781 ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac
     3841 gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc acgcaaggac
     3901 aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa caaggcggct
     3961 tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa gtgcaagttg
     4021 ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct gccaaaacga
     4081 cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc aatgcagctt
     4141 ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa aaaaatcaag
     4201 ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct atcccagaaa
     4261 atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagcta
     4321 ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa
     4381 gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg aagatcgtag
     4441 tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag atcaaagcaa
     4501 ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg
     4561 atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag cctttcacaa
     4621 atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag gagtatcaat
     4681 tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag
     4741 attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc
     4801 agatagctgg taattactca gcagagataa catggacatt agcagatgcg ccgacagaat
     4861 aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc
     4921 tctttactta tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc
     4981 gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca
     5041 tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac ggtctttaac
     5101 cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac
     5161 ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg
     5221 aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc ggtcactgag
     5281 acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg agggatcacg
     5341 tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt
     5401 tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc agatttgaaa
     5461 ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa
     5521 aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa
     5581 acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat
     5641 tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt
     5701 gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat
     5761 gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt
     5821 gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt
     5881 attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag
     5941 ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta
     6001 cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac gttgttccag
     6061 ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa ccaaatcagg
     6121 gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt
     6181 taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt actggagcac
     6241 aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat gccggttgga
     6301 aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg
     6361 gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa
     6421 tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca gatcaggcag
     6481 tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag
     6541 agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgctg
     6601 tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagaggaag
     6661 cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac tccctgaaat
     6721 ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg
     6781 gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccgttgaa
     6841 aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta
     6901 agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt gttttaaaga
     6961 agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt tcaaaccttt
     7021 gatactttca atatataaaa tttcccgagg atacaggacg agacttccac ctttttgatc
     7081 aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct gttctagcac
     7141 ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg gaaggatttt
     7201 ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa tattactatg
     7261 tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt gcttcaagtc
     7321 gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca actgattgat
     7381 ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca attctctgat
     7441 gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca tatgcaggct
     7501 cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa gatggtcgca
     7561 acagtgacac tttatttgac ctttactttg attttagatt taggtatcta tttattcttt
     7621 tcgaaacaac tgctagtaaa aaacagcttt ggttgtatag ctttgatggt ggtgacactt
     7681 ttacttccta tccctaaagt gagctttcct tacctagttg gcttattatt gatgtttctt
     7741 ttttatttta ctacgaaaaa cctgtttatc agctgtttga tcagtaatct tttttttgct
     7801 atcactggac tcagcttttt tctggttttt gatctgatgg attatttagg aaagagacat
     7861 cttggaacag caagtttttt tgcactactt atcgcattta ttttctatct attagtaaaa
     7921 agtatagaca aacagacgag atttctcaac ttgcttttta cttatccaac tgaacactat
     7981 tttggaggta tttgtatttt attaagctgg ggcgtgattt acttggtatt gggaagtgtt
     8041 cacttccgct catctaatta tctgcttcta tctcttttgt cgatagtcat cagttttttt
     8101 tctttgtttt tttccattat gttgatcagt ttccaagtaa aagaaagaca acatcaggaa
     8161 tcgcttcaac tgtatcaaat aaatgaagaa tattaccatc atctagagga attcaaacat
     8221 gattataaaa gtctactgtt ttcattaaaa attactcttc aggaaaataa aaaagaaacg
     8281 ttggaatttt tagaaatatt agaagatcat tctgcagcta tttttgaaaa accgcaaata
     8341 caacagctaa caaatattgt atccccagct gtcaggggtg tctttttaaa atttctagaa
     8401 aaagcagaat cggagaaaat cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc
     8461 ccgattgatc tggttgtttt tattcgctgt ttatctattt ttatttctaa cgcattcgat
     8521 catcgtgtag ccgatcagag tccgattacg atcaatctgc tggaagaaaa agagggatca
     8581 cgttttagta tagcgaacaa ggcaaatcca acgaatttga cgctatcgga gatggtcaaa
     8641 agaggaaaaa cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg
     8701 gatgtttacg aaaatgcaga actaaaaatc gaattttcaa aacagaatca ctattttttt
     8761 gtagaaatgt atctggcatc tagaaagagc aaatcagcct actgacttac tgtcaaagga
     8821 taagtttgag gaaagtctac acggagatag agacaacaac acagcatatt caaaagaaac
     8881 agaagcgtga tgaccgttgt cacccttctg tttcttttgc tatgtagtta aacaaagtag
     8941 ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact aagtagataa
     9001 gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta caccagataa
     9061 agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc tacgatcaca
     9121 aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat ccatcgaaaa
     9181 gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg aaaatcatta
     9241 agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa acaaaaagcc
     9301 tcaagggaag aagacacgct actgcataca catggtggct acctaaatta ttgattttct
     9361 atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca tctatttttt
     9421 atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga
     9481 atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg
     9541 aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat
     9601 ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag
     9661 atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca
     9721 ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac
     9781 gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa
     9841 aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg
     9901 ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa
     9961 cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca
    10021 aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa
    10081 ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt
    10141 gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat
    10201 acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat
    10261 tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat
    10321 ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca
    10381 ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa
    10441 gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa
    10501 ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc
    10561 aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag
    10621 ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga
    10681 ggtgcatctc tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt
    10741 atatgaaaag gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg
    10801 attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag
    10861 gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac
    10921 aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta
    10981 gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt
    11041 ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag
    11101 gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt
    11161 tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa
    11221 gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa
    11281 tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct
    11341 gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac
    11401 atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa
    11461 ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg
    11521 cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc
    11581 tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag
    11641 tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt
    11701 catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat
    11761 ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa
    11821 tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca
    11881 gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat
    11941 tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta
    12001 aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt
    12061 ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg
    12121 gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt
    12181 atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca
    12241 ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct
    12301 gcagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag
    12361 ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc
    12421 acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca
    12481 ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa
    12541 ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt
    12601 gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt
    12661 gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca
    12721 ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca ttggggattc
    12781 gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt
    12841 cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat
    12901 acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa
    12961 ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct
    13021 ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg
    13081 gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg
    13141 acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt
    13201 ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga
    13261 aaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca
    13321 ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt
    13381 gatccagaag taggagaact agttgcacct gcagacggag agatcacgac tatttttcca
    13441 actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg
    13501 atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc
    13561 gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat
    13621 agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta
    13681 gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat
    13741 caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc
    13801 aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg
    13861 gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag
    13921 aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc
    13981 ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa
    14041 tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc
    14101 tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa
    14161 tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct
    14221 atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga
    14281 ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa
    14341 gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa
    14401 gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga
    14461 ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga
    14521 tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg
    14581 catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg
    14641 aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt agttattata
    14701 tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg
    14761 gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag
    14821 ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg
    14881 aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc
    14941 ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag
    15001 atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa
    15061 tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga
    15121 atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa
    15181 acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct
    15241 ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa
    15301 aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg
    15361 aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt
    15421 cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa
    15481 gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc
    15541 ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga
    15601 tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc
    15661 tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta
    15721 tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg
    15781 tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt
    15841 tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat
    15901 acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac
    15961 ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa
    16021 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt
    16081 tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa
    16141 gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct
    16201 tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa
    16261 aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt
    16321 cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca
    16381 acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt
    16441 attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt
    16501 gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta
    16561 gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat
    16621 ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct
    16681 tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt
    16741 ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa
    16801 aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac
    16861 aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga
    16921 aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca
    16981 ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga
    17041 tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa
    17101 caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat
    17161 ttatcagtga aacataacac gagaacaaaa tgattttttc gttaataaaa gaaacaagta
    17221 tttgttgttt aaaataatac acgttattgg catataggac gaaatcccag tatcttctat
    17281 aagtaagtca tttcatatcg atgaaaacac gttatttatt tttgttttat ctataaaatg
    17341 gctgaaaaat gatttttttc taaatactat atatagtggc gtctatccta tataccacta
    17401 aaaaactaca aaatctagtc ttttatttta aaaaaacacg tgtagaatga agatagactt
    17461 ttgagttagg gagtgttttt ttatgaaact tactgttttt tgcggttccc gttttggtaa
    17521 taaagaaagt tacaaattta ttgcgcaaac gttaggaaaa tacatggcac aagaagaaat
    17581 cgaactggtt tacggtggtt catactccgg gattatggga gtgattagta aaacagtact
    17641 ggaaaataac ggaaaagtca caggtatcta tccgaatggg ctttttgaaa ctgaattgcc
    17701 tggaaaagat gttactacat ttattcctac agaaacgatc gatgaaagaa aggtattatt
    17761 atttgaaaag ggagatgctg tcctcgtttt ccctggaggt ctaggtacat tggaggaatt
    17821 ctcacaactt ctttcttgga tagcaatcgg tctgacgccc gacaagccaa tcggaatatt
    17881 gaatattggc ggttattaca gcggcttgca acaattatta gaaacttttg caaaagagga
    17941 ttttatggat aaaaaatggt tggatcaagt aatcttttcc aacaatccat tggaactggt
    18001 caatgaatta cgagcagtat ctattaaaaa tcagttatta ttgaaggagg caaaataatg
    18061 agtcaaatat atttagcagg accatttttt tcagaggagc aaatcgatcg agtaagtcgt
    18121 atcgaaaaag cgttggagga aaacaaaaca gtcacaagtt tctactctcc acgtcatcac
    18181 caagaaagca attatgaact attcagcgct ggatgggcgc aggaagtata cgaaaaagat
    18241 atggaagaac tgacaaatgc agaatttgtt gttgctatcc ttgactttga gcatcaaaca
    18301 atcgatccag gaacggctta cgaattaggt gtggcaacta tgttgaaaaa accgatgatc
    18361 attgtacaag aagagacggt acctacgaac ttgatgatta cacaaagtct ccatacgtat
    18421 ttaaaatcag accaagctgt acgtgaatat gattttgaaa cattgcctgt tgaaacttac
    18481 gtaggcgagt acttatagaa gaggaagggt tgtatgtcag tgattttgat ttcaggaacg
    18541 attggtgcag gtaagagcag tttgacggat atgcttgcaa aagagattga ttctaaacca
    18601 ttttatgaaa atgtcgaaga taacgaagta ttgccgctct tttattcaaa tcctgagcag
    18661 tacgcttttt tattgcaaat cttctttttg aacaagcgat ttttagcaat gaaaaatgct
    18721 ttagtgaacg atgacaatgt gctagaccga tccatctatg aagatagcct gttgttccat
    18781 ctaaacgctg atttaggtcg ggtaacggat atcgaagtac agcagtacga tagcttatta
    18841 gatacgatgc taaacgagtt agatgatgtt gctccaaaaa aacggcctga tttgatggtc
    18901 catatcaaag tttctttgga cacgatgcta gaacgtatca aaaaacgtgg gcgtgactat
    18961 gaacagctag aaagcgatga gacgttatat acctattatg aaacgttgaa cacccgctat
    19021 aatcaatggt atgaagattt tgatgtatgt cctaaaatac aagtggatgg ggataaattc
    19081 gattttgtag aaaatccaga agatgcaaaa aaggtgatcc gtcagatcca agaaaaatta
    19141 gctgaattgg aaggaaagcc tgctggaaaa tattttacgg ataaccagca tgcaaaaggt
    19201 caagaattag gctaaaaata aaaaaccaaa cgacgggaaa gaaaaattac tacgtgatgt
    19261 agaataagcc tttctatcgt ttggttattt tatattcaaa tatttgaaat aaataaatct
    19321 ttactctttg atttgtaaac tgaaatgatc cactgtttgt tttactgatg aagcttgatc
    19381 aaaggaagaa gctgagtttg aagttcataa aaatcagcca tagtgagttt tgcttggatt
    19441 tgtttatctt cttctacttt ttacataatt ttgaacagga taagattcaa atgttttaaa
    19501 tgaatctttg actagtttgt gacagttgtc gcatatgctc aagcttcctt ggtcattaaa
    19561 ttctgtatgg atatttttgt tgttattaca tgtacaataa tcaggactat cgatcatcca
    19621 tttcgtctcc tttacaatca tcttgcttct ttactatagc agaaaagaag gggagaaaca
    19681 aaagaagatc caacagacgg cactatagac tgtagatgtt gccatcctca tccttcaaac
    19741 ggtaattacc gatttttgta aatatgactg ttgtttcttt tttattttct tttcctgaaa
    19801 taatatattc gtcatctttg gtagtgatct tgatatctgt cagcactgtt ttttcttcag
    19861 atgattcaga agaatcagtt ccttgtgtct gatacgtgcg tgtccattcc atttttttat
    19921 ctgtaaatgt caactgtccc gcggataaat ccatatcttt acgtactttt agcgtatata
    19981 cactgttggt ggtatctgct ttttccgctt ttaagacaga agaatcggtt gtttcgtccg
    20041 aggattggtc agagccgcca aacaaatcac agccagtcaa tgtcaaaagt cccgcagaca
    20101 aaataaatag tacgattaat ttcttcaaaa tacttccccc atttgatagg ataaagaaat
    20161 tataccattg gaatgtaaaa cttcaaacgg atggaagaga aaaacgcaca aaacacccac
    20221 aaatagagta aattttagaa taaaatttta aaaaggcaaa aaatccgcca ctttcagaaa
    20281 gcgttttaaa aaagtgttgc aatcttttaa aaaaatgcta tattaaaaat gaaccggttc
    20341 aaatacttgt ttaagtataa ttaatagtga gaggaagttg ctctactgaa gactttagtt
    20401 atatggatat tgatgacaag caacactatg caaagaatgc ggtcatcata atcgaacaac
    20461 aagaaagaga aatcaaatgt aactggcagg agtgagaatg tatggcatcg atccgtgatg
    20521 tggctaagat ggctggtgtt tcagtgggaa cagtttcccg ttacttgaat ggtcagcaac
    20581 tgaaggaaaa gaacatgaaa aaaatagcag aagcgatttc agcgttggac tataaagaaa
    20641 acatcattgc taaagggttg aagaataatc gaagcttctc tattggactt ttgataaacg
    20701 ggatttccag ccgttttggg tcggaagttg tctcagggat cgaaagagta gctgaagaaa
    20761 atgggtatag cttattatta agcgggtttg ccgatcagcc agaaaaaatt gaacaaaaaa
    20821 ttgaatacct gatgaagcat gtgatcgatg ggctgattgt cttcttatcc gaagaagagt
    20881 ggtctggatt tgaaatgctc acaaaaatga gtattccagt tatcgcattg aattgtccag
    20941 aaggaccagc aggtattgat acaatattag tgaatgatcg agaaagtgta gcaaaagtga
    21001 tcgctcatca tggtgaacaa ggacataaaa aaatcggatt tattgctgcc actcaaacag
    21061 actacgtagc aagagaacgt ttagcaggag ccaaagaagc ggtccaaaat gatccatcga
    21121 tccaattaga ggtctttaca ggagactatt ctagaaaaag cggctattac ggggcaaaag
    21181 cattattaga aaaaggggca actgctattt ttgtcagcaa ttacaatatg tcgattggtg
    21241 ccatcgaatg tttcaatcaa gaaggtgtca gaatcgggaa ggatattgcg ttcagtcact
    21301 atgattattt agataaaaac gatctgagta tccttccgaa aatcacgatc acgccaccaa
    21361 cagataaaat cggggaacga gcagcagaac agctgttaaa acgaatcaaa aaagaagagg
    21421 aagaaaatga aaaagtgatt gtgatggaaa atatcataca tggaatcaac taaaacgaac
    21481 taaaaaagaa aaagaggtgc tttgatgaaa aaatatcaat ttccagcaaa tttttggtgg
    21541 ggttctgcag caagtggccc acaaacagaa ggaaggattt caggcgatgg aaaaggagag
    21601 aacatttttg attattggta caaaaaagaa ccgcaaaagt tctttgatca agtaggacct
    21661 gaaaaaacct cgcaagttta tacgaagtat caagaggatg tacagttgat gaaacagact
    21721 gggcataatt catttcgaac atctatccaa tggagccgtt tgatacctga tgaatctgga
    21781 acaatcaacg aagaagccgt tcgtttctat catcgttatt ttgatgagtt gattgaaaac
    21841 ggtatcgaac cctttgtgaa tctgtatcat ttcgatatgc cgatgtatct gcaagaaaaa
    21901 ggcggatggt tgaaccgaga aacggttgaa gcctatgaaa aatatgccaa aagttgtttt
    21961 gaattgtttg gttcgaaagt aaaaaaatgg tttactcata atgaaccaat cgttccagtc
    22021 gaagcaggtt atttatatcg atggcattat ccagaagagt cagatatgaa aaaagcgatc
    22081 caagttggct atcatgaagc gttggcttca gcgttagcca tcaaatctta ccatgaaatg
    22141 gagcaaggtg gtgttattgg tatcatcttg aatctgacac caagctatcc aagagacgaa
    22201 tcaaaccctg aagatgttaa agcagcgcaa atagcagatg cgttcttcaa tcgttcattc
    22261 cttgatccag ctgtaaaagg cgagtttcca agtgaattga ttgagatagt aaaagaactt
    22321 gatctcatgc ctgtgatcca tgcagaggat ttgacggtta tcaaagaaaa tacagtagat
    22381 cttttaggta tcaattatta ccagccacgc cgggtaaaag caaaagaaac accaatcgat
    22441 actaaaaacg gtccaatgcc tgaggattat ttcgacaatt atgaaatgcc tggtcgtaaa
    22501 atgaatcctt atcgcggatg ggagatctat gaacaaggaa tctatgatat tttgatcaat
    22561 gtacgagatc attatggaaa tatcgactgc tttatttctg aaaacggtat gggtgtagaa
    22621 aacgaagcac gatttatcaa agaagatggc atgatccacg atgattatcg gatcgaattt
    22681 gtccaatctc atctgaaata tgtccatcaa gccattcaag aaggtgccaa ttgtttaggc
    22741 tatcatatgt ggacatgtat ggataactgg tcgtggacca atgcatataa gaatcgatat
    22801 ggctttattt cagtagactt agcacaagat ggaaaaagaa caatcaaaaa atcgggtcat
    22861 tggttcaaag acgtatcaga taaaaacgga tttgaggctt aagaaagaat agttgagagc
    22921 aaatgcgtga aattctccat tcttaccatt ttgtgaaaaa gaagttcaat tatttacgat
    22981 ctttttcaac ttttttgtct ttctatttca ttttttgtat ataatagaat agtcaaaatt
    23041 aaataggagt ggaggaaagc atgaggaaaa aaagaccgtt tcttctcttt tggttattcg
    23101 gttttttctt actgcttatt ttatccgcag cattttcagg aaaaatgaca gcagatcttg
    23161 ctcagaaaga aacagaaaaa atcgtagcaa gcaaagaatc agtagataat ttcaaaaaag
    23221 aattctcaac ttattggtgg gcggagtata aaataactgc caattacgaa aaaggcgaat
    23281 tggacattta tttgccaaaa ggaaaagaag aaaatcaatt tattaatatc gatgacgtac
    23341 ttagtgcgat ttctctcaga caatatcaat ttaaagacaa gcatttgacc gttttgcttt
    23401 tgactcatga tggcgacaaa gtagcccgtt tgaaaaatgg aaaagtgatc accgcttatt
    23461 ctgaaaaagt agacaaaaaa gtggtcgccg atcgtttagc ggaatggaaa aagacaaatg
    23521 aacaagtcaa gaaaacaact gtctcttctc aaaaaaacga gtcgacagga acaagtcctt
    23581 cagattctgg cagtggacaa acgataacgg gaagtgaaga aaatcaagcc tcaattatta
    23641 cgaatgagtc atcacaagga caatatcaat cctatggaca atgaaaaaca gaggttgtga
    23701 aatcagcgtt ttgacctgag tattagagaa acaaacggca aaatagttcc tcatattttg
    23761 ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtgtatca agaggtcttg
    23821 agcctgccgt ttaactctga gccacaagga gcaatttcaa aaaatagctc cttatatttt
    23881 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt
    23941 gtaaaattag cgtcctgaca taatctaata ccgagggtca gctgattgaa caccgtgtat
    24001 caagaggtct tgagcctgcc gtttaactct gagccacaag gaacaatttc aaaaaacagc
    24061 ttctcatgtt ttataaatat tgtgacttgt ggccgaggag ttggcaggag aacaccgttt
    24121 aaggaagaag ttgtgaaaaa gctgtcctgc atcaagaaat aaggagctgc gacaagaatt
    24181 ttgtcacagc ctcttttttt gtacgaatac cgagaaactg gcttttgaat accgtggagt
    24241 gaagcggtca ccacaagact tttatctctt cattagttta atcgcttaca tcgcagcggt
    24301 tttatgctgt gaaaagtgga cggaataatc tttagaaaga ataaaaaaat cttaagataa
    24361 caactttttt aggaaatttt catttttcag gagcaaaata agaaaaaatg tgaatagaga
    24421 gaagtgatat acgtgacaca acttaaaaaa ttgtgccaaa atcgtttagc aatcgttttg
    24481 acagctgttt tctttttctg gctgaagacg atcattgcct actatgcaga tttttcatta
    24541 ggtgtggaag gtacgatcca gtactttatc ttgtggatca acccaattgc gactacctta
    24601 ttgttttttg gcctttcttt gtatattaaa aagccaaagc cagcgttagc tattcttttg
    24661 atcattgata ttttaaatac gcttttgttg tacttgaata ttatttttta tcgggaattc
    24721 acagatttca ttacagtcaa atctgtatta ggcttttcga aagtgtcgca aggattgtca
    24781 ggaagctcct tttctttgat gaagccccat gatgtcatct actggctgga tatcgctgtg
    24841 ttcattgggc tactggtttg gttgaaggtc aagaaaatac ctatcaaaag caaccctgtc
    24901 ggcaaaccga tggctatcgc tgttacctgt ctttcagggc ttattttttc aggaaatcta
    24961 gcgctgagcg aagccaaccg tccacaattg ctgcagcgaa catttgaccg ttcatatatt
    25021 gtcaaatatc taggaatcga tgcgtatact atttatgacg gaatcaaaac aggaatgact
    25081 agcagtgttc gcgcgcatgc aagcagtaat gggattgatg aagttttaga ttatacaaag
    25141 aaacattatg cagagccaaa tcccgaaaca tttgggatcg ctaaagggaa aaatgtgatc
    25201 gttctccatt tagaaagttt ccagcagttt ttgattaata tgaaggtaga cggacaagaa
    25261 gtcacaccgt ttttaaatag tattttccag aatcaagcaa cgatcagctt tgacaatttc
    25321 tttcatgaag taggacaggg aaagacaagt gatgctgaaa atatgttgga aactggcaca
    25381 tttggtttac ctcaaggctc attattcaca gaacttgggt cagacaatgt ctttcaagcc
    25441 gcaccagcga tccttggaca aaagcaaggc tatacaagcg ctgttttcca tgggaatgta
    25501 gctagttttt ggaatagaga tcatgtatat aaaaacttag gttatgacaa tttctttgat
    25561 cgatcttatt ttgatgaatc cgacgaaacg ttaggttatg ggatattaga taaagattta
    25621 ttcagagaat ctgcccaata tcttgagcat ctacaacagc cgttttacac gaaatttctt
    25681 tccgtaacca accacacgcc ttattatacg gatgacaaga atttcgactt tccatctttg
    25741 aatactggca atagcacagt agataattat gttcgaacgg cacattatct ggatcaatca
    25801 ttggaacaat tttttacgta tttgaaaaaa tctggtattt accaaaattc aatctttgtg
    25861 atttacgggg atcattttgg gatctcgaat acagataata aagatttagc aagcgctctt
    25921 ggtaaagatc cagatacatg ggatgaattt gacaacgcac agatgcaacg agtgccattg
    25981 atgatccata tgcctggtta tactaaaggg acagtcaatc atgaatacgg tggagaaatc
    26041 gatgttttgc cgacattact acatctattg ggaatcgatg ataaagatta tcttcatttt
    26101 ggtacagact tattatcttc tcagcatgat caagtagttg cttttcgaaa tgggaacttt
    26161 gtgacaccca aatacaccgt cctaggagga aaagcttaca ataatcagac aggagaaatc
    26221 atcgatacga aagcagcagg aatcgatgag gaaatctcca aagatcagga aaaagtcaag
    26281 actgcgttaa gtctttcaga taagctgaat caggaaaatc tcttgcggtt ttatgtgcca
    26341 aacggatttg agacgattga tccgaaaaaa tatgactata aaaatgaagc ggaacgtaat
    26401 gaagcaatcg aaaagcaaaa aggcgaacat tcgacaagtg tcttttcaaa aaatggtaat
    26461 aaaacaacaa ccaacttata tccagtaaca agaacagacg ttgaacagca aaaaaataat
    26521 caataaacaa tcaaaccaac cttcagtagt tagacaacat tctaatgctt gaaggttggt
    26581 attttttatg aaa
//
LOCUS       DAZUKR010000025        26259 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000025 DAZUKR010000000
VERSION     DAZUKR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.025"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..535)
                     /locus_tag="ACG89C_000952"
     CDS             complement(<1..535)
                     /locus_tag="ACG89C_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HFS4899524.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPK"
     gene            complement(985..1605)
                     /locus_tag="ACG89C_000953"
     CDS             complement(985..1605)
                     /locus_tag="ACG89C_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4899525.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(1607..2476)
                     /locus_tag="ACG89C_000954"
     CDS             complement(1607..2476)
                     /locus_tag="ACG89C_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4899526.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(2499..2906)
                     /locus_tag="ACG89C_000955"
     CDS             complement(2499..2906)
                     /locus_tag="ACG89C_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4899527.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(2925..4214)
                     /locus_tag="ACG89C_000956"
     CDS             complement(2925..4214)
                     /locus_tag="ACG89C_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4899528.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(4241..4543)
                     /locus_tag="ACG89C_000957"
     CDS             complement(4241..4543)
                     /locus_tag="ACG89C_000957"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4899529.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(4568..6661)
                     /locus_tag="ACG89C_000958"
     CDS             complement(4568..6661)
                     /locus_tag="ACG89C_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4899530.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(7150..7914)
                     /locus_tag="ACG89C_000959"
     CDS             complement(7150..7914)
                     /locus_tag="ACG89C_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS4899531.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(8025..8894)
                     /gene="mdcB"
                     /locus_tag="ACG89C_000960"
     CDS             complement(8025..8894)
                     /gene="mdcB"
                     /locus_tag="ACG89C_000960"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS4899532.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(8919..9995)
                     /locus_tag="ACG89C_000961"
     CDS             complement(8919..9995)
                     /locus_tag="ACG89C_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4899533.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(10019..10612)
                     /locus_tag="ACG89C_000962"
     CDS             complement(10019..10612)
                     /locus_tag="ACG89C_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS4899534.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(10616..12292)
                     /gene="mdcD"
                     /locus_tag="ACG89C_000963"
     CDS             complement(10616..12292)
                     /gene="mdcD"
                     /locus_tag="ACG89C_000963"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS4899535.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(12282..12581)
                     /locus_tag="ACG89C_000964"
     CDS             complement(12282..12581)
                     /locus_tag="ACG89C_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS4899536.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(12683..14329)
                     /gene="mdcA"
                     /locus_tag="ACG89C_000965"
     CDS             complement(12683..14329)
                     /gene="mdcA"
                     /locus_tag="ACG89C_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS4899537.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(14336..15247)
                     /locus_tag="ACG89C_000966"
     CDS             complement(14336..15247)
                     /locus_tag="ACG89C_000966"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4899538.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            15367..16224
                     /locus_tag="ACG89C_000967"
     CDS             15367..16224
                     /locus_tag="ACG89C_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4899539.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(16411..16773)
                     /locus_tag="ACG89C_000968"
     CDS             complement(16411..16773)
                     /locus_tag="ACG89C_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4899540.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(16962..17144)
                     /locus_tag="ACG89C_000969"
     CDS             complement(16962..17144)
                     /locus_tag="ACG89C_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899541.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            17119..18558
                     /locus_tag="ACG89C_000970"
     CDS             17119..18558
                     /locus_tag="ACG89C_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899542.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(18565..21117)
                     /locus_tag="ACG89C_000971"
     CDS             complement(18565..21117)
                     /locus_tag="ACG89C_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS4899543.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(21231..22562)
                     /locus_tag="ACG89C_000972"
     CDS             complement(21231..22562)
                     /locus_tag="ACG89C_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4899544.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(22528..24006)
                     /locus_tag="ACG89C_000973"
     CDS             complement(22528..24006)
                     /locus_tag="ACG89C_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4899545.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(24311..26146)
                     /gene="tdc"
                     /locus_tag="ACG89C_000974"
     CDS             complement(24311..26146)
                     /gene="tdc"
                     /locus_tag="ACG89C_000974"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4899546.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         7606 a         5624 c         4503 g         8526 t
ORIGIN      
        1 gctttggcaa ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa
       61 acttggcctc ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct
      121 gtggaatatc tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg
      181 ttaatcctgt ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt
      241 cagcaaatcc accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct
      301 tgccatcttt tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa
      361 tcgttgtgag cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca
      421 atgaaatcgt ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata
      481 gaccagcttg agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct
      541 tcccttcttt tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta
      601 cggcaaataa atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca
      661 aaagacattc gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg
      721 taataagccc caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa
      781 ttgaggcagg acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct
      841 cggtcacaag tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg
      901 tgactcggag ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt
      961 tttcgatatt aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc
     1021 caatagtgga gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc
     1081 taactggaat ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat
     1141 atgattggtc cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat
     1201 atagctcaaa tgacgatctg attcacgatc acgtttgtac cgacgttgac ataacacttc
     1261 aaagtctgct gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg
     1321 gagtcgtgct ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc
     1381 ttccatatac aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc
     1441 cgcccgttct tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc
     1501 gagcgtgatg gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt
     1561 tcctgtacca ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta
     1621 agttaattgg actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta
     1681 ctgcttcatt tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa
     1741 aggtttcttt agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc
     1801 ggatcccgtt attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga
     1861 taactagggg gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg
     1921 tttttgattt gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg
     1981 tagcttctac aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga
     2041 catggattcc ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt
     2101 gcgcatgggc atattctact actgcttttg tcttcaggat attttctttg aaaggcagat
     2161 gggacccatc atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa
     2221 agtctcgggc gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg
     2281 tcattacatt gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa
     2341 taatgatcgg cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt
     2401 tatgcgtatt aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt
     2461 tcaatgttgt atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt
     2521 acttgttttg caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga
     2581 tcatcaccag cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta
     2641 tacccatctc cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga
     2701 tcgttcacat acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta
     2761 tgcttatctt gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt
     2821 tcggtcgctt ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt
     2881 tcttttttct taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa
     2941 actgggctta agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct
     3001 gtagcattga tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta
     3061 ataaagaaac catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg
     3121 ccaaatatcc ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc
     3181 gctaagccga acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg
     3241 aaacctaaaa tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg
     3301 atcgcatcag gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac
     3361 agtagacgta caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa
     3421 aaggcaaaca tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca
     3481 aataaaacag tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct
     3541 tgcagaaatt cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct
     3601 ttctcaccaa aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga
     3661 cctaaagcaa tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc
     3721 atagcaatgg cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc
     3781 agaccggcaa aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca
     3841 gttaaaaaaa tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg
     3901 gctaagaacg tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg
     3961 atagattcaa tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg
     4021 aaataagtta aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg
     4081 agtgtcttta acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc
     4141 aacatagcaa tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa
     4201 ttgataacgc ccatgttttt accctctttt ctttttgtat ttagataact cccttatctt
     4261 gcagaactgg ggtgattttt tcttcaattt cattttttga aacaatattt ttcaaataga
     4321 tcatcgccgt tttaggatca actgcgaact tttcgaactg cgaacgaaaa ttttctgctg
     4381 tgatgatcac atctggtttc atggaaactg cggaagaaat atcacaatgg tccagtttgg
     4441 cttctactcc taatttgttc aaaacatcct cagcagacat ctgtgctgca aaactacttc
     4501 ctaaacctgc accgcatacg aataaaatgt tcaatttgtt catctttgtt ctcccccgta
     4561 tcatttatta tttctcaact actgaaaaaa gcagttgttt gaaatcttcg atatttgtgc
     4621 attcaatcaa tcgatccaac ttctcttcgt tgttgatcaa ttcaattagt tctttcatga
     4681 tgttcagatg cgagtaggag tctactgctg ccaaacaaaa aataatcttt actggatcca
     4741 tttcaggatt accgaaatcg attggttgct caatcgtagc tacgctgact cctaatttat
     4801 tgaccccatc ttctggtcgg gcatgggcta gtgccatgtg tttgccaatc acgatatacg
     4861 gtccgtaatc attcactgca cgaatcatcg cttcgacata gcgctcttcg acaatgtttt
     4921 ctttaaccaa tggtctagca gctagttcaa tcgattcctc ccaagtagtg gctttttctt
     4981 tgatcatgat attgctgtcc gtcaaaatat cttttaacat aggctggatc tcccttttgt
     5041 tgatttctaa attgtttgtt tcgaataatt tttctaactt gccatagatg attcgatcta
     5101 ctcggccacc actttctttc atgacatcta ttatttgagt gaataactcc gtcgattcat
     5161 gactgttagg ctctaaccgc tgtagttctt catttctttt caaaaagtct tctataacca
     5221 agatattcgt ctcagttaaa atcggatcga caactaaaag cggcttattt cgataattca
     5281 attgaaaggt gctgaaaacc agattgacat ccaatttatc tactaattcg atttcttttg
     5341 aacttaaaac tgcagtaatc tcgatctgag gaaacttttc tttcagattc tctgctaaca
     5401 gatttccagt agccatccca tggttacaga caacgactga tttataaaca taattcaagt
     5461 ctcgcttgat tacactggcg atcgtcgaaa agtggatgac aagaaaagct atctcgtcct
     5521 ccagtatctt gccaccaatt acttcttcga tcgtaggaac aaaatgagtg actgcgcggt
     5581 agattgggaa ataatttgtt cggatgtttt ctttcaatgg attcatgact tggatatgat
     5641 tcttgattct tacaatcagt gcagccatgt gcttatagag attttcgaat aaagcttctt
     5701 cctttagatg gaaaggtacg tgtgtctcgt tctctacaaa ttggatcaat tggatcgtta
     5761 gtaattgagc ttgtacccac tcgattgaat tggagatatc tctagcattg aacgtctcaa
     5821 tagtgaaacg aagataattt ctttctactt gcgggacatt gtccaattga aattcctcga
     5881 tcatgtggtc aataaatgca gaaaagctac tgtcttttat ttctgtattt ttccaattaa
     5941 tggcagagat aaattcatgt ttcctgattc gctggatcca gatcaatgta aaaagcagga
     6001 tttgtttttt ataaatatca tcttcttgtt tcaacaactt attggaatag atctcctgta
     6061 tttttttgat ttccaccaaa ggaaaataat ggagaaagat tttttgagcc ggtgttaatt
     6121 gtgcatcttg attcataaag aagtcggtca ttcccagatt ctgattgata aaatcataga
     6181 tcatcgtacg gatagatcgt tctgaacctt tataaaccag tccttgcttt ccataactga
     6241 cgatctctat cccataactg accagtgttg ctcgtaaccg tctcatatct tcatctaatg
     6301 tacttttaga gatttgaaat tcttcttctt ttttgtttag atggatcggg attcggctgt
     6361 aagcaacggc taaaactaga tcaaaaatac gttcgcgccg attcaaaaag ccgtctttca
     6421 catcttttat ttcagtttct aatcgagctt gcttttcctt ttccagcttc aacaaaaaaa
     6481 ctttgttgcg aattgtacgg atctccggaa gctttttact gctcaaccag atattcgctt
     6541 caagaacttc ctgacggatc gtccgctgac tgacattgaa ctcacttgct agatgttttg
     6601 cttttactgg atattgggct gtcatgattc tgtgaagtat ttcaattgtt cttgttttca
     6661 tagaaatgat ctccttctga tttcaagtat atggttccgc ttacataata aaaagtagat
     6721 atttctgcca cttgaagtgg aaagaaagta agcaaagcaa acaaaaaata agccatatgc
     6781 tttttcatct cctcaacgaa aaagacatac gcctattatt acaaaaattt tgcccatcta
     6841 tacaaaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca actaaactaa
     6901 aatagtgctt cctatttttc gtttggttgt tcttattatc gcagatgcaa gcttttaaac
     6961 accatttagt ggagaggttg tgacagagat gttcagcctt gagcgataag aagcgatttt
     7021 gaaaaatcgc ttcccatatt ttacaaaatc gtgccttatt gccgataggc tatctctggc
     7081 acactgttta ttcagaaaaa ggagctgcga caaggctttt gtcacaactc cgttcataaa
     7141 tatttcctgt caatccagca taatcggctg aataaaatcg gcttgccatc ttttttcttt
     7201 ctcagaaaaa tcaaaatacc atatctgaga aaaatactgt cgttgtgtct tacttcctga
     7261 gatcaatcgc ccatcttttc gatcgactga ataatcacag catctgaaat caattcggat
     7321 ggcaaaacta ttttcgtgga tctgtcgata atttcctact tcctgaatag ttattttttt
     7381 agtatgattt cttacaccgt ctgctttgtt tttttgcaga actttctgat gttcggcaaa
     7441 cagcttgtct gtataacagc ttctggcttt ttcaggagtt tcttcttccc aagcggattg
     7501 gatcttcatg aatatctttt ggatttccag aaactgtgct ttcttttctt tagccgttcc
     7561 tgacatgcgg gacaatatct ccgctttcag ccttttcttt tgtcttttat tcttgacgat
     7621 agaaaaagca gaaacccctc ctactgacaa aacagctaat gcaagaaatc ccgtttgctc
     7681 ataactagaa ggacctgtac ctggaccata atacccataa gaatctctgc cgtaagaacg
     7741 actgccaaaa taactgcccc catatgaacg accaccaaat gaacctctgc ccgaacttcg
     7801 accgctagat gtacctccac catgaccacc cgcgacagcg aatccttggg tcggcagagc
     7861 aaacaaacat aggcccacta acaaaatgat tgctaaataa ttgatttttt tcattgtctt
     7921 cctcgttatc ttttattaca aaaaactatt tttatgatag ccttgctaaa aggaagagtc
     7981 aataaccaga tgcataaact gtttttttag caattttgtc ttatttagtc attgagtaac
     8041 atcggacaaa tgtatgtgtc ggcagtccat aattcctcta tcttcagcat gaacaagctt
     8101 gcagacaaga gatctgcgct tcctccagga gagatttggt tttcctgaca aaagtgcaac
     8161 agtgctgtaa atggacgatt tggtaaagca ctttgactag cagcatgagc aagtttttgc
     8221 atttccacta acgttttctg atcactgcga tgcaatacgc atgtgtcatc tagcgaactc
     8281 atcaaaacta acaacatatg catttgtttc tgtacatatg aatcattatc tagctcctta
     8341 gctgcttgga ttgcaagcct gatatgcgga taccctaata tcgcttcttc atatgcacca
     8401 tttacgccat atttttgctt caccctatga ccatgagtgg tttttttatg acgatatttt
     8461 gtatcaggaa aagcagcaag cttgctgaca tcatcaagta ttttcgataa agaaaaaggc
     8521 tgttccatac tgattttttg tgcaatgaca ctgatcaata gccccattgc ccaaatcgct
     8581 cctttatggg tattgatgcc atttgtagca atcagcattg ccttttccgc ttttcgccca
     8641 atctcagcaa ttctttctct taactcctga tcaatagcat gatgccatgc tatttgagcc
     8701 atttcttcaa aaaaaggagt tagcgtctta gcagacttaa gaaataaagc atagtccata
     8761 tcggaatgtg cacccgcatc cagtgcatcc actaatccag gttttggact taatgttact
     8821 tctgcgatca atgcttcctc taccaacgca gcgagttgct gagtcactgc tttttttcgt
     8881 tcgtcattta tcatgacact cactccttct tcctactatt acttgctgaa accttttttg
     8941 atagctaccc aaatgatatg aagtaaccag cttaaagcaa atgcacaact tgccgtcatt
     9001 agataagcta caagaatcat gactcctgaa ggtaactgca gccaactatc tttaaacaat
     9061 aataacagta cttggaaaac aaatacattg gctaaatacg tacgataagc ataaacagac
     9121 aaccatttga acaatggcaa ttttggcgaa tcgtttacaa tcattttgga agcgcctatg
     9181 aagacagcaa agatgatgac taaactgtat aaactttgga tcgtattcaa gtaaggtgca
     9241 ttcgcaaagc tcaagtctcc tggatgtgcg agcaatgaac ggacagaaaa gaacgcgata
     9301 actaaggcaa caggtaagaa aagataacgt actctgtcca aatattgata gactttttcg
     9361 tgataaacaa atgcggctac cccataaatc atgaaactag caacaaaccg atctaataag
     9421 taccagctgt catgataagg cccagtcaat acttgtgttt gatagaacag ataccatgcg
     9481 acatataaaa tcgttgaacc aatcaatact ggccatacat attttttatt agcaaatact
     9541 ttgtaaccga gccaaacaaa aaacggcatg aacaattgga tctgcagcat cataacggta
     9601 taccataaat gaggcgctcc atcacctgtg acatatttca gtaaaaagct tgcagtatcc
     9661 gtatacggag tttgttgttg gactgctgga aatagccaaa gataagccgt cgtccataga
     9721 agatacggaa cgactagttc aaaaaacttt tctttcaaaa attcccaata agacaaatgc
     9781 tgacttgttt ttaccatatt ataaacaatt gcaaaaataa acattggtgc tgtgtattta
     9841 acgatgacgt aaaaggtacc aagaaaagct gtatttgttg cgctgtggct gtttgccaaa
     9901 gcaaagctca atatcgtttg catcataaca gccgttacag ctgatacttt ctggaagtca
     9961 cttacgttga ttttgatttt tttgttttca aacatctctt tctctctctt tttctttatt
    10021 accaaagttc tttggttaat ttaggtcctt cgactgtttt caatagaatt tttttctctg
    10081 gctctctcaa atactctttt agtgaaaaac cgccttgccg agtcactact tgggtatcga
    10141 attgctcaaa aaaagcagga tgcgattgaa ttacctgaat tggcaattca atcggtgaat
    10201 ttgcgtacag cagcaaatca aaatcactgg tttcttttac tgccggaatc ccagtcgata
    10261 attcaaatcc taagctgccg ccgattcccc atttacatcc tgataatatg tttctagcag
    10321 catgaaattg ctgataaaca ggataatcca tgattttctc ttgtctatac gattcttgtt
    10381 cgatgatgtc ccacggatga atgacttgtt tgacggcatg ttttgagatt tctgttgacc
    10441 atctctgatt gcgggcaggt ccacgtaaac caatcggtag tatttctgcg tgatccgtcg
    10501 acgcacgacg aactgttccg aacatctttt cagttagaac gacccattca ggcaaggtga
    10561 acccttctat ttcatgttta tcaaaaagga caatatcatg tgcttgaatc attttttatg
    10621 catcccattc ttcgtccatt ttggcacgga ctttatttgt ttcgacacgt ccgaatgttt
    10681 tggctgtttc gtttgtgtat cgatttgtca gcatcgtgtc atttgtattg cggacatctt
    10741 gaatggcttc attgattgct tcgatgactt tgctgacatt ttgttctgaa gcagcttggt
    10801 cggtgatatc ttctaggaaa tgatagatcg ctcctagttt tgtataatta ttgatatcgt
    10861 aagcgatagc tggaactttt tcagcagctg cttcgatttc agcaaccgaa cgttttgtca
    10921 cacgtgcagc actttcttta gacatcgctt ggacggtaat ttctttattg tttaaagcaa
    10981 tcaagcggct tgattgtaat ccatgtgcta aaaatgcacc tgaaatagca tttcctgcaa
    11041 tcaatccaat aactggatgt ccagcttgac ggagttttgc ataagcagca gcactattag
    11101 ccaaagccac atggataccg atcaattctt cgttatagcc gtatgcttgg cttggtacat
    11161 cgatcacaac gatgattggc cgtttcactt ttttgtcttt atcttcttcg taaacgtgat
    11221 ccaaaatttg tcccatgcgg aaaccttcaa ctaaaccaac ttcaccgttt cgtacacggt
    11281 acatcgggtt tttaggatca ggtacgattg atgtcacgat ggcttcttct atgaatttac
    11341 ttgatttacc ataatacaca gtactaatag aagaagttgg atttttcatg ccagttagtg
    11401 ccgcaaacca atcgtaacca acacttgctg cagctggttc atttccttct gctacttctg
    11461 gtaagtcaat tgattttgct tgatggttag caattgcttc attgtaatcg tcaatggcca
    11521 ttggttttga aagatctaaa gaatcaagta atgataaata aaatgctgag ctttcactgc
    11581 gatgcgtgtc ttttcgttcg tccattgctg cgatgacagc agctttgatt gcttctacac
    11641 tatcagtcac taattcatca atgatttttg attctacccg ttgacgagat cctaatgtat
    11701 tccaaatcaa gtctttgtca ctagaatcaa attctcgaac gcctgcttct tgttcaataa
    11761 cttctggtcc attcaaaccg actctcgctt tttttgttgc gattaaataa ctcattaatg
    11821 ctgaagtaat cgacataccg ccaaatgatc ctacacgtcc tggaatcaaa ccgatgactg
    11881 gtacatattg ttttagagca atgattacat ttccgatttc tgaaatagat aacagtccgt
    11941 agtttgcttc ttgcaaacga acaccacctg tatctagtac aatgactgga aagatctcat
    12001 ttcctttttt attttcttct aacacatgtt caagtgcagc aacgattttt gcaccggaaa
    12061 cttcaccgat accgccacct tggaattttc cttccatagc gattactact gcttgtttgc
    12121 cattgatcgt gccacggctg acaacgatcc cgtcatcact ttcagggacg attccttgtg
    12181 ctggtaaact tggcgcgatg atttgatcga atggatcaat caattcacgt ccattgtcaa
    12241 gtagagcgtt cactcgttct ctggcgttta attctacaaa actatttttc atctgaaagt
    12301 tcctccattg cttgcgtcaa tcgtaggtac acaactcctg gtgtagcacc gaagtcattg
    12361 atcacgatat ctgctgaaat tggataacga tcaaaaaatc tcgttaatac attgttccat
    12421 acttcgccga agccatcgct tcctgtacaa atttttacag tagattctgt tttttctgtt
    12481 ggaaacatca agatttccaa atctcctgaa ccgaccactc ctacatggat tttcttattg
    12541 atcggctgtt tcgttggata tttataagct aattgttcca tggatctctc ccctttttgt
    12601 aaaacagagc atacacgttc tgcttattcg tctttcttct tccatctggg taaatggcga
    12661 caaaaggttt ttctcgccaa ttttatgacc aagttctgaa tttcattggt ggttcataca
    12721 ggccatctga ccattcgatc aaatcatcga tggtttgtgc agctaataat gaacgtttcg
    12781 cttcactacg tttgatgcct agatcttctg gtaatttcac gatgccggct gcacgcaagc
    12841 tatctagtga ttctttcgtg ctagtcattc caagaggtgt aacaccgcca attgctgcaa
    12901 ttgctgcttg tcgttcttct tttgtttttg ctttgtacaa ataagcaatt ccttcttctg
    12961 tcacgatatg tgtagtatct tcgctataaa tcatcactgg tgcattggct aagcctgctt
    13021 gtttttgaac agctagtgca tctaattctt cgacaaagac tggtttttta tttgaaccat
    13081 atgtttcaac catttgaaca actaattttt ggcctttccc taatggatca tcattgtcgc
    13141 gaagtgattg ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag
    13201 gcgcaccgcc aaaaccagat aaacgtcctt ttgttacggt agaagagttt ccttcgtaat
    13261 ctaattgcaa ggttgaaccg ataaacatat cgatggcata ttgtcctgca gcttgtgata
    13321 atgttcggtt actgcgcatc gtgccgtctt taccgacaaa aaagacatct gggcgagcag
    13381 caatatattt ttccatacca acttcgccac caaaactatg gatgctttca acccaacctg
    13441 attcgatggc tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc
    13501 cttttaaccc tagtgattca ccataagttg gcagtaacaa ttcgatagct gctgtattga
    13561 atccaatacc gtgattcaaa gattgtacgc cgtgttctgc gtagattcct ttgattgcca
    13621 tcattgccat tagaatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt
    13681 ctaattgata aggtttatct gctttcacga caacgtctac ccagtcacca gggatatcta
    13741 cacgtggtaa agaatcgaca acttcgttga cttgaacgat cacgatacca tctttgaaag
    13801 ctgcagcttc gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt
    13861 ctttatctgc tttatctgca gctactaaaa caacatttgg aatcaaatcg atgaataagc
    13921 gtccataaag ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca
    13981 tttgggcgac acgcaagctt tgtgcaccag cataagagaa atcgatcttg cttgcgattc
    14041 ctttttcaaa aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat
    14101 cattcacttt ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt
    14161 gattgtcgcc ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta
    14221 cgattttttc agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct
    14281 gcgctttttt ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc
    14341 tccatttttg atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac
    14401 ggatcccttt atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg
    14461 gaaattcaat cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact
    14521 gcacagggaa agctaggttt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg
    14581 ctcttccatc cgtatttttt atatacaaac atctagcatc agatagatgg acagactgca
    14641 tatacggctt gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga
    14701 ctttcagcag cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata
    14761 aaccttcgat actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa
    14821 ctggatggtc aggatcaaat gtttttgcta caatttcctc agcttcgctt ctcgtcacac
    14881 ctactactac gcccattgca taaccgcttg gataagcttc tttcattaag actgcgcggt
    14941 gatagactag ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa
    15001 aagcaccaat cgaatgtcct gctgcaatat caggctgaat accgtttttt tccatttcat
    15061 ctaaataaaa tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt
    15121 tttcttcttt atcaactaac cgataccccg tcacattttc taaacgttcc atgtacattt
    15181 cagggatatc cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg
    15241 tactcatgag cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct
    15301 gctataataa aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg
    15361 ataattatga aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt
    15421 acccaagcag ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat
    15481 ttggaaaacg aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc
    15541 gtttatgcag aagaatttta tccatatgca gtcaagacat tagacacatt agaagaagga
    15601 atcaaattat tgtcttcggt caatgagaag atcgttactt ccgtcatatt gtatttagag
    15661 gtagcctctg tttctattcc gaacttggtc cgaattttta tggaaaagca tcctgatatc
    15721 caattaacga tcatgcaaca tcatttacca gatgatatta ccgatcctgt actgtacatc
    15781 acttcggaga tgaagcctgg catgaccaat attcctatca tgaaagaacc tgtttatgtc
    15841 gccattccta agaaacatcc tcttgctaaa aaagaacatc tgcaattaga agatatccaa
    15901 cgcaccccca tcttgatgct gtcaaagagc aacgccttcc gtcatacact ggatgcagca
    15961 cttgaagcga aaagtatcga attgacgatc agttcgacga cagatgatcc tgcgacatta
    16021 cgttctattg tcaagcaagg attagctgtt agctttttcc caaaagtttc ttggtcttac
    16081 gataaaaatg atcctttcgt cttgcgggaa atcaaagatt tgcccttgac acgaacaatt
    16141 tatttatctt ctagcttcac agaagatcat cctttgatca aaatgattgc aaaaacgatg
    16201 cgggaatttt atttaggaaa ataaaaaaaa aagagctggg acaaaattct tgtcgcagct
    16261 cttttttcga acaaacggtg ttccagaagt aatttcttag aaaataagcc gaaatttccc
    16321 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagcta aacatttctg
    16381 tcacaacctc ttctctattg tcctagacat tcattgcgtt gatttaaaac ttccatcaat
    16441 caaatctgct agttcatcta gcttttctat tttacgtaga tcaatagaga tccaatgttc
    16501 tttattcatg tgataggctg gaaaataccc ctcttttcct cgcaaaattc cgccaagttc
    16561 aggatcgacc ttcaaatcaa gaatattgat gatcctatgg tcacttgcat catcatagat
    16621 tttttcttct ggaacagaca tcaggacccc aaaccatttt ttgctttttt gatgtctgaa
    16681 tacgcaataa tcaggaaatt ttttaaaaat atattctgga gacaaattgt atgtttcatt
    16741 gatgtgatcg attagttctt ttttcgttag catctgcttc catctctttc ttattgattc
    16801 gctttattca aagataccac gtaagaatag aaaaatgaat acaagtagtt gctaatcaaa
    16861 tacttcgata tttttctttt ttatcaactc ttaaatgttc aatccgcctg tgttggtctt
    16921 gctaccatct actattaaaa tgtttattat tgtttgccca cctatttttt gattttagtg
    16981 gtatcatttt tcaagttcat gatgaaattc ttatcatcag taaatgctcc agaaaaataa
    17041 acaatgattg aaaaatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa
    17101 tagaagggag tctgattgat gtattcacta atgaaaaaaa tcattaccga aaaagatatt
    17161 cgacgtcatg tttctttagt agaacaattg ctgaatcata caaagataac cgtaaatgaa
    17221 ttagctgaaa tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc
    17281 tctcagctgc ccgaaggctg ggacatcatc agcgatcaag caggaatcag tttgcaaaac
    17341 cagcaaaatt tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtc
    17401 gaactgctga aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagccgac
    17461 catgggcttt cttatgggac attgaaaaga catgtgacta aaatcaatca gcgtttagca
    17521 agttacgact tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt
    17581 gcgattcgta ctttttatca tcgattgctg attcctttca ctcataacaa ctattttttc
    17641 gaggattatt cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact
    17701 gaactagcgg ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc
    17761 cgtataaaag cgaattgccg attagattct tctatccata ccaacagcac actctcctcc
    17821 ctgtatgact ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact
    17881 gagctttctt ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga
    17941 caagaaaccg ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta
    18001 gaggaactag cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac
    18061 ttggctctcc tcatattaaa atatcatgaa tcgccgatat tgattgaagc attggaccgt
    18121 caataccatc gttttttaga aacatatgat cagcaatatg cacacttgta tcaacagaaa
    18181 gagaatttac tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa ttactttctc
    18241 caactcttgt ctctactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat
    18301 gtctatttta ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa
    18361 gattacttag gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt
    18421 tcttttgaaa aaacagatat cttgatttcc aatttccctc tagattctct agatatccct
    18481 attgtataca tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact
    18541 ttcaaaactt ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt
    18601 gcgaaacagt ttgttcatga tccctttttc tatttctaca actaagaata gaagaatcac
    18661 atttgctcca atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg
    18721 gccgactggt agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa
    18781 aagcgctttg ttttgcaaaa gaccttggtt caatgacggc ttggacaact ctctgcaatt
    18841 gatcatgtaa acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct
    18901 ttgcccttca aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata
    18961 gatgatccga aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg
    19021 cttcatgatt tctggatctg gcgactcgaa ccctagtgca taagataaag tgattgtcgt
    19081 taccatattg acccataaaa tctgtactgg ggacaaaggt agcgggcgat tcagcataag
    19141 tgcccaaatc aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt
    19201 ttgcaaatta tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt
    19261 atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat
    19321 agcaattcct acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat
    19381 tccgacgatt tctccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggtgt
    19441 cgttcgagca aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag
    19501 atccaaatcg atcccttcta aaactttggc agtatgtttt aagccaattt gttccgcaat
    19561 cgcttttgct gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg
    19621 gcattccttt acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc
    19681 tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt
    19741 ataagcaaat cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca
    19801 tgcttcttgt tctgcagaag aaagagttga tttagcaaat aatacttctg gtgcaccttt
    19861 gacgtaaatc accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga
    19921 actgaacgga atctttgctt ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg
    19981 aatcagcgca acttctgtcg gattgccact taaattttct gttttctgtt cttcttttgt
    20041 ttttaattcc tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac
    20101 atccacaact gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt
    20161 catagaacct aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat
    20221 ttcatgtact ccaacggata gaatcatggt caaaacagct ggcaatcctt cgggtaccat
    20281 tgacaccacc aaagcaatcg tagccgaaag aaggatatgc caatccatac catatcgaaa
    20341 tgtcgtaaaa ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt
    20401 taatcggttg atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg
    20461 attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc
    20521 agattgtaca agcgttcctg aaaaagccat atttttctgg tcgccgatcg attcttcttc
    20581 tgtcaaaacg gcatgacttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc
    20641 cactaataaa tcatgtgctt cgattaatcg gatatctgct ggaacgacat ctccggcacg
    20701 taagacaatg acatctcctt ttaccagttc tttcgcaggg attttttctt gcatcccgtt
    20761 tgatagtact atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg attcttccgc
    20821 ttttctttct tgccagtaac taacgagtcc attgacgatc actacgaata aaataatact
    20881 tccttcaata tattcactag aagcaaattt caaaaaagag gcaatgatca acacgatcat
    20941 cagcagatca gtaaaatgct ttgctatttt tttccatcgt tttgtctgct gccttgcttt
    21001 tatttcatta tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc
    21061 tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct
    21121 atcccctcct gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg
    21181 attatttcag gggggtctga aataattcgt tttgcaaaat gtatctgggc ctaactgata
    21241 tttctattgc ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa
    21301 aaaggaataa agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac
    21361 accaacaaag caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat
    21421 tgaacatgtg cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct
    21481 gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccggca
    21541 aaaaataaca ttcctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca
    21601 caaaaccaat ggggcaaact tttttctttt gctgtttgat ataaaatcct gctagtactg
    21661 tagactgcac tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct
    21721 gctccgcctt ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct
    21781 aaacaatgcc atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagaga
    21841 atacgccaag gcagacagtt gattgctttt ttcaaattct tttgcggatc acttgtctcg
    21901 ccaacggtta agccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgag
    21961 actaccattc cagcaaagcc aaacggtagt aattcatcga tctgtccaaa attttctttg
    22021 attccgatag actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa
    22081 aggaaaatcc ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg
    22141 actgcaacaa ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct
    22201 ggtacatgta caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca
    22261 gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa
    22321 taatagtcaa cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc
    22381 atggcccgca tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct
    22441 ggccctgctg atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca
    22501 cctaatgcga ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc
    22561 atcaagttca cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt
    22621 ctttattttt tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc
    22681 aattagattg atccatgttc cagctttcat cgtcagtccg acagaaaatc cgattgccgc
    22741 aaataaaaca ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat
    22801 tctaaatggt cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat
    22861 gatgacatag acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg
    22921 attgacgcca ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt
    22981 caaaataacg gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg
    23041 atacgcacct tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga
    23101 taattggact gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg
    23161 caaaccaaga atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg
    23221 tccttttggt acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa
    23281 aattaacgcg gcaaatactc cgcgaatata cgttttaact ggattttcca atcgagtgat
    23341 atacacagag gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa
    23401 tgaggatgca gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc
    23461 ggttggtacg atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa
    23521 aggcacgtac aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt
    23581 caaattcaaa agagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct
    23641 gttcaaacca agcggcacat tatcgataat attaccaaac aacggagcga gtgctgaagc
    23701 gaccatcacc atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca
    23761 tttttttcct agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc
    23821 agcaaattca ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat
    23881 gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac
    23941 aagggcgcag gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc
    24001 tttcatcttt attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt
    24061 caagaacaga aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat
    24121 ccattgaacg atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga
    24181 acgttttata aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat
    24241 tgttttgatg aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca
    24301 aaaatcagtt ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttttt
    24361 ttgtataaaa attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta
    24421 gcagagtgat tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa
    24481 aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc
    24541 gttcaccata aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt
    24601 cattgatagc gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa
    24661 aatcaggatg attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat
    24721 gatcgagaaa ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat
    24781 atccggtcac attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc
    24841 cagcttttga tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt
    24901 caaacacata tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga
    24961 ttcctccagc agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg
    25021 cttctgaaat agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga
    25081 aaacatgatg ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt
    25141 cgtttagaaa cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat
    25201 agatcccttc atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt
    25261 gaccttcttc tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgtccag
    25321 ctaattcacg aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg
    25381 ggatagcaac catctgatct aagccgactc cgcaaatatc taacgccttc atccaagaat
    25441 agtgttttgt ttgagggacc agccatttcc ctaatctttg gatatttttt ccgctacggg
    25501 aagaagctgc tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa
    25561 tttcttcaac agataaattc aaaagttccc actcagacat ttttttcact ttttcaggca
    25621 gcacttcctt gacagctaaa ggaatcgatt tgatgcaacg ggcgtaccat aatccttcaa
    25681 gattagcaat agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg
    25741 aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg
    25801 caacattatt tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg
    25861 tttcagcatt catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc
    25921 gtaatcgctg actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat
    25981 ctggagtgag cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc
    26041 tccatccaag atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt
    26101 cagctttgtc gcctataaag acggccttga tatccatatc tttcattttt cttccctcca
    26161 tattttgttg gtcaatgtct tgcagataca tgtgtttatc tgtaacacct cttcactata
    26221 gcgccaaaaa tgagaaattt ttttttataa catcaaatc
//
LOCUS       DAZUKR010000026        26134 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000026 DAZUKR010000000
VERSION     DAZUKR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.026"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..310
                     /locus_tag="ACG89C_000975"
     CDS             <1..310
                     /locus_tag="ACG89C_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4899547.1"
                     /translation="QIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNL
                     IEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF
                     "
     gene            783..1337
                     /locus_tag="ACG89C_000976"
     CDS             783..1337
                     /locus_tag="ACG89C_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4899548.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1510..1947)
                     /locus_tag="ACG89C_000977"
     CDS             complement(1510..1947)
                     /locus_tag="ACG89C_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4899549.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1995..2942
                     /locus_tag="ACG89C_000978"
                     /pseudo
     CDS             1995..2942
                     /locus_tag="ACG89C_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3213..3648
                     /locus_tag="ACG89C_000979"
                     /pseudo
     CDS             3213..3648
                     /locus_tag="ACG89C_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3664..4497
                     /locus_tag="ACG89C_000980"
     CDS             3664..4497
                     /locus_tag="ACG89C_000980"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4899550.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4742..5767
                     /locus_tag="ACG89C_000981"
     CDS             4742..5767
                     /locus_tag="ACG89C_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4899551.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5905..6030
                     /locus_tag="ACG89C_000982"
     CDS             5905..6030
                     /locus_tag="ACG89C_000982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899552.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            6055..7170
                     /locus_tag="ACG89C_000983"
                     /pseudo
     CDS             6055..7170
                     /locus_tag="ACG89C_000983"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            7253..8149
                     /locus_tag="ACG89C_000984"
                     /pseudo
     CDS             7253..8149
                     /locus_tag="ACG89C_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            8165..8989
                     /locus_tag="ACG89C_000985"
     CDS             8165..8989
                     /locus_tag="ACG89C_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899553.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9021..10331
                     /locus_tag="ACG89C_000986"
     CDS             9021..10331
                     /locus_tag="ACG89C_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFS4899554.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10479..11738
                     /locus_tag="ACG89C_000987"
     CDS             10479..11738
                     /locus_tag="ACG89C_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4899555.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11748..12620
                     /locus_tag="ACG89C_000988"
     CDS             11748..12620
                     /locus_tag="ACG89C_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899556.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12632..13462
                     /locus_tag="ACG89C_000989"
     CDS             12632..13462
                     /locus_tag="ACG89C_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899557.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13502..15685
                     /locus_tag="ACG89C_000990"
     CDS             13502..15685
                     /locus_tag="ACG89C_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4899558.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15746..16966
                     /locus_tag="ACG89C_000991"
     CDS             15746..16966
                     /locus_tag="ACG89C_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4899559.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16963..18423
                     /gene="gtfA"
                     /locus_tag="ACG89C_000992"
     CDS             16963..18423
                     /gene="gtfA"
                     /locus_tag="ACG89C_000992"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFS4899560.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18500..19606
                     /locus_tag="ACG89C_000993"
                     /pseudo
     CDS             18500..19606
                     /locus_tag="ACG89C_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19928..20137
                     /locus_tag="ACG89C_000994"
     CDS             19928..20137
                     /locus_tag="ACG89C_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4899561.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20130..20279
                     /locus_tag="ACG89C_000995"
     CDS             20130..20279
                     /locus_tag="ACG89C_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS4899562.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20433..21098
                     /locus_tag="ACG89C_000996"
     CDS             20433..21098
                     /locus_tag="ACG89C_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4899563.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="ACG89C_000997"
     CDS             complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="ACG89C_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS4899564.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22801..23034
                     /locus_tag="ACG89C_000998"
     CDS             22801..23034
                     /locus_tag="ACG89C_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFS4899565.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23031..23438
                     /locus_tag="ACG89C_000999"
     CDS             23031..23438
                     /locus_tag="ACG89C_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4899566.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24575..25093)
                     /locus_tag="ACG89C_001000"
     CDS             complement(24575..25093)
                     /locus_tag="ACG89C_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS4899567.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25460..25708
                     /locus_tag="ACG89C_001001"
     CDS             25460..25708
                     /locus_tag="ACG89C_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899568.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8921 a         3984 c         4844 g         8385 t
ORIGIN      
        1 tcaaataagg tttatgatag ataaatggaa aaagcagtat ccacgagtgg ttaaattgct
       61 catgaatcct gctatattaa ccttttataa tttccctccg tcaatcagaa gaactattta
      121 ttcaactaat ttgattgaag gttttaacaa acaattaaag aaatatacca aaagaaaaga
      181 gcaatttcct aatgaagaat ctctggagag atttctagta tctcaattca acaactataa
      241 tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa atacatgata cattagaatc
      301 aatgttttaa cttgatcaac agaatgggct tttccattta cacaaaatta ttgacgctcc
      361 cgacaaaatt atgatcttgc gtaaagttat aaaatattaa taaaaatatt ttattaatga
      421 aaagcttggt agcacggtta ttcatatgct aatttatttc actttatagt aaggggagtt
      481 cttagtatat tgttcaaact taaatttcat ataatttaat tatgtaaatt agctattcta
      541 agaacttcta cggataactg cccgtctaaa atttaaataa gagcttagaa tcttcatata
      601 agtaaggtta atttttttgt acaaaaaagt cggaatattc tgagttactg aaatgatata
      661 aaatcataaa ctaaatccct tgattttatt gactttcaaa agtttatata tataagttaa
      721 tgattatggt aaaatatata cataatcatt aactcagaat atttttaaga agggaaacaa
      781 tagtgagcta taatgtacaa ccgttaagga caaatgaaga gatacaagat tttcagtttt
      841 ggctaagaag aacaagtaat ccggaaagag atagtttttt atttttgttt ggaatcaata
      901 atgggttacg tatgtcagat attataggat taaaagttgg agatatacga ggcaaaagta
      961 agcctattat tgttgaacat aaaacaggta agcgaaaacc gatttttata gataatttga
     1021 gagaagaaat tttattatat actgaaggta aagaagaaaa tgattggtta tttccgagtc
     1081 ggcaacaagg tcgacatata acaagggatc gtgtctatca gatatatgca gatatagcag
     1141 aaaaattagg acgagatgat ataggaacac atacattaag aaaaactttt gggtatcact
     1201 actacaagaa aactagagac attgctacct taatgtttat ttttaatcat agctcgcaag
     1261 caattacaaa aaggtacatt ggtataactg aagatgaaat aggagcatca cttcgtggat
     1321 ttaaactagg tgtataattt aaaaattttt ataaacagta aagacaaaga ttaaaagcta
     1381 tttttatcta gaacgatttt atttcataaa gttcgcggaa taaaagctgg ggttactggt
     1441 acttggtata ggttcgtgtt atataaacta agtaattaaa taacattttt aggttggaaa
     1501 aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa
     1561 gaaaaatact gataactcaa cgtttagcag actatctaat agggatagtc tgcttttttc
     1621 gtttttgacc atacttttga ccttctgcaa acttccttgc ttttcctctg tagaactcat
     1681 gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat
     1741 atcatccata gtggttttaa tatctgtatg tccaagtcga acatacactt ctttgacaga
     1801 cgcaccagat tcaaagagca gactacaatg ggtatgacga aaaccatgaa gtgtgatatg
     1861 ttttaagtta ttctctttaa tgatgatttt caaattgtga tttacatagt ctaaataaag
     1921 gggcttattt tcttctgatg aaaacaaaag ttgttcacca tcttttatag gtttgattct
     1981 catttttaga tattatgccc gcacgtattc atcacatttc tattgtgaat cgctttattc
     2041 gaccgacatt tgacttttat cataatatac tcggtttaaa attactaatg aaaacaatta
     2101 atcaagacga tcataccatg tatcacctgt ttttttcaga taatcaccac cgtgtgggaa
     2161 ctgaactgac tttttttgaa gtacaagaag ggaacaacca gttttttgga accaatacga
     2221 ttgaacgaac gatactaaaa gttccttccg aagcatcatt gcatttttgg gaaatatatt
     2281 ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt ttctggacga cctatcttac
     2341 gttttgaagg gccagatgca acacgattag ctttagttcc cttacgcgaa tttgaagata
     2401 ttgatgatta tttcccgtat gtcacagacc agatccctac agagcatgcc attttaggaa
     2461 ttgatgccat tcaactacgc gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg
     2521 taggctggta gcacaaagaa tacgtcagtt tcttttcggg tcatcaacga gtgacaatct
     2581 tagaaaatca agacactcag ttctatcaag aagttcagat tattcaagat cgtgagaatc
     2641 cagttgccat tgaagggatt ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat
     2701 ctgatttaca acgcatcgac aaacaattac aagagcgcaa ttttataaat tcaggaataa
     2761 aagatcgtga attttttgta tcactttatt atcgtgaccc aaatcaatta ttgattgaaa
     2821 ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg
     2881 aagaaattcc tttattttta ccgcagtact taaatttcat tcgtgaacaa gtggagcaat
     2941 aattagctaa tcaaatccat gacgaagagt aagaggttga ctcataagtc aaaaaataaa
     3001 gaaaaacgca tcaaatccat tctaaagaat gttgatttga tgcggttcct atttttataa
     3061 actatcattt tcgactaata aaactactta tgagtcagcc tctctttttt tcttgatttt
     3121 tatacctctt tctaggtaaa ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt
     3181 ctcaactatt tcaaaataaa ggggagtacc aaatgaacga gtatattgaa attatccacg
     3241 caagagaaac aacttaaaaa tgtcagtctc aaaattccca aaaataaaat tagtattttt
     3301 accagcgtat ctggttcagg aaaatcatcg attgtttttg atactattac ccaagaagct
     3361 ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt tatttttacc taaatacagt
     3421 cgtccagagg ttgatgagat tgataatctt tctacggcga ttgtggtcaa tcaaaagaag
     3481 attggtggca gtgcgcgctc aacgttaggg acgattacta acatcaatgc gttatttcgt
     3541 gtgttgtttt catgttttgc aacacctagt ttaggatatg caaatgctta ttcatttaat
     3601 gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta agacgattgc atttttaata
     3661 ggattgcttt atttttcggc tattgataat gctgattttg tattttctga tccatttacc
     3721 gcagaagttt tgtctggaat tgaggaacag gcaaagaatc atggttattt tgtattggtg
     3781 cataatgtta attcagcaga agatgttaaa actattcaga gaaattggcg ttttgagggg
     3841 ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat taaataacgt ttttgatggt
     3901 cctgttgttt atattgatac gcatctttca aaagagacat taagagaact tgaacaacaa
     3961 atgaatcgtt tatttgtaaa tacaaatgat taccaagcta gtacattggc agtggaacat
     4021 ttgattcatt atggtcatga gaaaatagcg tttctttctt ttgactacat tgtaggtgaa
     4081 ccaagtgtta ttcaagaacg acatcaagct tatatcgata cattaaagaa atatcattta
     4141 acctttaaat cgcattggat atataacaac tctgaatttg agcgaattta tcaagaatta
     4201 gctgaattta ctgcaattgt tgttactgga gattatttag cggctaaatt agtaaaattt
     4261 ttaaaaaata agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt
     4321 acttttgcag aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag
     4381 ggggcgattg cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa
     4441 gtactactat tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa
     4501 agctctgtga aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg
     4561 tgatctagca gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt
     4621 cggatatttt ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt
     4681 attttcaaaa tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat
     4741 tatggtagga attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg
     4801 cgtgctaaat gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga
     4861 atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat
     4921 tgcattaata acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat
     4981 tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg
     5041 tttgtcagaa acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat
     5101 tgggcaggtg ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt
     5161 gattgatgac cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc
     5221 aacgaatcag catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg
     5281 aggaggacgt gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg
     5341 aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta
     5401 tttagaaggc tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca
     5461 tgctaaagat aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt
     5521 ttatcgttca cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt
     5581 tgataatatt gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac
     5641 agaagaaatt ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa
     5701 tgtttcgatc aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat
     5761 cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta
     5821 gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac
     5881 gcattcaaaa caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata
     5941 gctgttttaa ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac
     6001 gggcaagtaa ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa
     6061 atgttagatg aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct
     6121 tgggatcaat attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta
     6181 gtaaccatgc actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt
     6241 gatgatgtta tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat
     6301 ttctatacgc ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta
     6361 tggtacaaca aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca
     6421 tgggaaacac tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt
     6481 tttgcatcac atattggaac agagactggc tactggccat ttatttatca aaatggggga
     6541 gaagtatttg ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct
     6601 ttagcttttt atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta
     6661 caagatcctg ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga
     6721 aactggtttg caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc
     6781 gtgttaccta agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt
     6841 gcgagtacaa aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac
     6901 gccaataaaa tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac
     6961 gagtgggctg cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat
     7021 ggagtccctc gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt
     7081 attaaccgta ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa
     7141 gaagtcaatg cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa
     7201 ggattctatt tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa
     7261 tcgaattttt ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc
     7321 aacggcatta ggattaattt tattaaatat atggccagca attcaaacag caattttaag
     7381 ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa
     7441 aatgtttgca gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt
     7501 ttcgatacct agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt
     7561 taaaggattg tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc
     7621 agtcgcaatg atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt
     7681 ggctaaaata ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc
     7741 tattatcatt gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg
     7801 cttataagaa attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat
     7861 ccagcagttt ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt
     7921 aacaacagtt atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa
     7981 tagtaccgcc atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat
     8041 tttaaatgac aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat
     8101 tattactgca attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca
     8161 aattatgaaa aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg
     8221 tattgcaatg gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga
     8281 atctacccaa attccaccta caattttccc tgagaaattt cagctaagta actatcaaca
     8341 agtacttgat ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg
     8401 acgagtgatt ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag
     8461 ttttccaggg aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc
     8521 catttttatt actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc
     8581 agcattaata ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt
     8641 cttaggttta cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac
     8701 gatctttttc aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt
     8761 cacagcgttg tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga
     8821 taagatgcct ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc
     8881 acaattaatg gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt
     8941 ccaaagaaaa ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt
     9001 agtgattgga gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt
     9061 ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg
     9121 cattgtacga tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta
     9181 ataaaaattc gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt
     9241 taagagaggc tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag
     9301 tgattgattt tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg
     9361 ggattggtgg gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag
     9421 aaatggaaga agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt
     9481 taaccatggg tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc
     9541 aagtatgcgt accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg
     9601 actttcaatg gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa
     9661 atggggaaga tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc
     9721 cagattctgt acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg
     9781 aacacaatgc agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg
     9841 aattagggat tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg
     9901 aaagcatgcg agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat
     9961 atgcttcata tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg
    10021 tcttaaataa aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt
    10081 tagtcgataa aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg
    10141 ctttaaatcg gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag
    10201 ataaatcaaa aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt
    10261 taacagatat caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg
    10321 aatataatta aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag
    10381 ttaaatttag taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa
    10441 aagaaaacgc attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta
    10501 ttttagcttg tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg
    10561 aagatgcaag cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa
    10621 cgattcagga gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg
    10681 tggatgtacc gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag
    10741 atgtcattaa tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt
    10801 tagaggactt aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt
    10861 ttgctattga ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact
    10921 ataacaaaac tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg
    10981 aaaaaatagt agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag
    11041 aaggctggac tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag
    11101 aggaagcgaa caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag
    11161 aaatgcaaaa agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca
    11221 actggcaagg tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga
    11281 ttatgccgaa tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag
    11341 taggaacttt tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag
    11401 acctagcctt atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag
    11461 aatatatgac aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg
    11521 ctgttgaggg cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg
    11581 cttttactga tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt
    11641 ataccttaac aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa
    11701 atgctttctt taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag
    11761 aaattttaaa tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga
    11821 tttttttcta ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc
    11881 tgacttataa ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga
    11941 agtttatcaa atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa
    12001 ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct
    12061 ttagagcatg gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta
    12121 aacaaatttt taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac
    12181 aaaccagtct tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt
    12241 ggcaaggaat tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag
    12301 aaattattga agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg
    12361 aaatcaccta cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct
    12421 taacagcatt tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat
    12481 ctttggggtt acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg
    12541 ctattgcttt agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat
    12601 cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc
    12661 taaatacttg cttttattat tagggggctt catgatttta gttcccttag tttcaacgct
    12721 attcggttca tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc
    12781 agcgactttg gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa
    12841 ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc
    12901 ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt
    12961 aattttagga atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag
    13021 taaaattggt ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc
    13081 acagacacta tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga
    13141 ggctgctgaa attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat
    13201 gttaaaacca atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt
    13261 catgctacca ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca
    13321 atataattat acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt
    13381 aggaattgtc acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat
    13441 gagcaatgga gcagtgaaat aaccaataaa ttattctcat tataaaggag ttcataaact
    13501 tatgattata tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta
    13561 tctattagga atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac
    13621 gcactattct ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc
    13681 aaatgcgcaa gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg
    13741 acatggtgat tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa
    13801 tgattttcgt tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc
    13861 agccttatat acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc
    13921 gattagtgga ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat
    13981 tcgttctacg caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag
    14041 tcaatcaatt gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc
    14101 aagagaacgt caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa
    14161 acgcggatca agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga
    14221 tgaatttcaa ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt
    14281 gattcaaaaa gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt
    14341 tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag
    14401 taatgaaggt ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat
    14461 tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta
    14521 ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat
    14581 tgaaatgttt gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt
    14641 aggtgattgg gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca
    14701 agtgcatgat aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga
    14761 tagtgaacta tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc
    14821 aattagtcga aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta
    14881 tcaacaaatg acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa
    14941 tcgaaatatg acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc
    15001 tcatcgttat atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac
    15061 tattttattt gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta
    15121 catgccacaa acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata
    15181 tggaaccagc ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa
    15241 tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt
    15301 ttttggttat gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga
    15361 acaagttgcc ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt
    15421 ggaaagtccg tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga
    15481 agccatggtc ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa
    15541 attagcagga ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga
    15601 tgaattgatg aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca
    15661 acgcttttat ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa
    15721 gagactcatt ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc
    15781 tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg
    15841 gttagataaa ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact
    15901 agagtatggc atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat
    15961 tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc
    16021 acataatagt atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat
    16081 tggtgaagat ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc
    16141 tgaaactggt gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat
    16201 ttttacgaat caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca
    16261 cgatcaacca cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa
    16321 aaaaatgttg gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca
    16381 agaactagga atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac
    16441 gcattctcag tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc
    16501 tgtcaataaa cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa
    16561 tgccagattc tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact
    16621 caatgcagaa gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat
    16681 taaattaaga aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt
    16741 gaaagataaa aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt
    16801 agttatctta aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca
    16861 agtattaatc aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg
    16921 accgtttgaa agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa
    16981 gcaatgctca tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc
    17041 ttagacaaac atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg
    17101 acaggtgatc gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca
    17161 tgggaagatg tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat
    17221 cacatttctc gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca
    17281 tacaaagaaa tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa
    17341 gatattgatt taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg
    17401 gatggtacga cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg
    17461 accaaagaag ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc
    17521 tgttctttaa ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat
    17581 ttcttcgtgg aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa
    17641 tatgacgtcg aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc
    17701 aatcatgatt attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat
    17761 agtggaaaat cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca
    17821 acattggata cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa
    17881 gaattggatt acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat
    17941 tcaagtgcca attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca
    18001 ttaggcgata atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg
    18061 attccacaaa tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa
    18121 gcaacgaaag aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa
    18181 gaagtgcaac ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct
    18241 gcggcgtttg atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg
    18301 attacacgta aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg
    18361 aaagaattta tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt
    18421 taacctattc catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac
    18481 caataaaaag gactcataca tggataagta taaaacaaat acagcattta ataaatggtt
    18541 ttctgccata aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa
    18601 ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa
    18661 tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact
    18721 agaaaccgat gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga
    18781 acctaaagtg ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa
    18841 accaaccaag cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa
    18901 acgtcataag tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta
    18961 ttgttatatg gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga
    19021 acatgatctc aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga
    19081 tcgtgggtat taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt
    19141 cactcggatt aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga
    19201 aacagaaatt ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa
    19261 tttccgacta gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg
    19321 aatggacgtc tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt
    19381 gtttttcaag cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa
    19441 aggcgtgcag aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa
    19501 attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact
    19561 gtttcagccc tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt
    19621 ggctcgttac ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat
    19681 cctctgcgtc agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt
    19741 ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt
    19801 tttgagtatt aaggagcaag tatacaaaga tttataaagc tgattaaatt tgaataaaaa
    19861 tatgcaggaa cgctactatt tttagtagtg ttctttttta tatttgaata aaaaatgtgg
    19921 tggttaaatg gtggttataa caaaaaatga attaatgaaa ttaggttatg ggaagtatca
    19981 gtctgagaga atcatacgac aggcaaaagc aattatggtt tcaaaaagct atacctatta
    20041 cgaaaataga agattaggac tagtacctgt tgaatctgta gaagaagttt taggtatgaa
    20101 gatagaggta gaggaggatt ttcagaatca tggttaattt taaaaaagat aaaaatggga
    20161 aatatcattt tactgctagt ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa
    20221 acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat
    20281 tatggaaaag aagcagaaac ttctcaaagt actatgttgt ttgaggagtt tttttataaa
    20341 ttattcctac cttattatca agggaaagta gaagaaagaa catataaaaa tcggttatct
    20401 acaatcaaaa aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg
    20461 acattcaaaa gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca
    20521 tgtatgggat ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa
    20581 atcctgcaaa agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa
    20641 aagaagaatt tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg
    20701 gttttatttg tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat
    20761 taacttggaa agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt
    20821 ataagagagc caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa
    20881 tcgaactaga cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa
    20941 taggaggcat gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc
    21001 gtcatattat gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat
    21061 taaggcatag tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag
    21121 aaaggttaaa acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa
    21181 attcattgag agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa
    21241 tcaaaccgac acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac
    21301 caaaagaaac acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt
    21361 ccgcctattt atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc
    21421 tatatgggta tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa
    21481 taccaatgaa tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg
    21541 gctgacttga attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc
    21601 ggatatacgc ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt
    21661 tactttatat cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc
    21721 tgctatattg acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc
    21781 taaagatagg tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa
    21841 aatcagtgca ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg
    21901 tttactcaaa gtatttttat attcattggc aatataatct agataatttt gtgtatcctt
    21961 atctagaaat aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt
    22021 gatggttgct acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt
    22081 ggaacttttc gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt
    22141 agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt
    22201 tacattttct tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt
    22261 taactgtctt atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc
    22321 ataaaagagc ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc
    22381 ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat
    22441 tttttgatat atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga
    22501 gtatctctat aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa
    22561 atttattaat ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata
    22621 aatggtatac ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc
    22681 ttaattaatt atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc
    22741 ttgatttaaa atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact
    22801 atggcaatta aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca
    22861 aaggagtttc ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa
    22921 aaacttcgag cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata
    22981 tctcaagcat taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc
    23041 attacctaga agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc
    23101 ctaatgaacc ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc
    23161 gagcctcagc ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac
    23221 ttatacaatt gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag
    23281 ctacttttgt ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg
    23341 taaatttagc ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag
    23401 tgatcaacac tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta
    23461 tagggttatt agctgctata gcgaaataat tgaagtaaat tacaaatagc tataattcaa
    23521 atccaactat atagaaaacg taaattttct atatagttgg attaagataa cgactattta
    23581 ttttatcata tttttactat accctatact tttacttggt atatttatga ataatcgcaa
    23641 atttacccct atactactag tttgtaagtt agattaataa aaatactaaa gttatttgtt
    23701 cttttattaa atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata
    23761 aaaataacag ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt
    23821 gcgtatgaac ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat
    23881 cggtactggg attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta
    23941 ctatttgatt tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt
    24001 atagaattta tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc
    24061 ctttgatggt tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa
    24121 caaaggctat ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc
    24181 ttcctaaccc aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg
    24241 ttgagattcc atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa
    24301 aaataaaaaa atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca
    24361 taatctcatt ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa
    24421 tctaacgatg caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa
    24481 ccggcagtta gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc
    24541 tcctttccac aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc
    24601 ttatctttgt tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata
    24661 cttcccatac agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata
    24721 gatcttcatc actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa
    24781 tttcctctaa ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact
    24841 ctcctaactg attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt
    24901 catcattttc ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag
    24961 caggtgttct atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa
    25021 attctaaaat atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt
    25081 tatatgttct cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata
    25141 atttcatcca cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct
    25201 aactcatatg tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg
    25261 tcagcaagga gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc
    25321 atatcatttt cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac
    25381 taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc
    25441 attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg
    25501 ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac
    25561 gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa
    25621 tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca
    25681 agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat
    25741 ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca
    25801 gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa
    25861 tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt
    25921 tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt
    25981 tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct
    26041 aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca
    26101 acttatatgt tttagtgaac tacccccact tagc
//
LOCUS       DAZUKR010000027        25527 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000027 DAZUKR010000000
VERSION     DAZUKR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.027"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..2140
                     /locus_tag="ACG89C_001002"
     CDS             80..2140
                     /locus_tag="ACG89C_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS4899569.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2223..2792
                     /locus_tag="ACG89C_001003"
     CDS             2223..2792
                     /locus_tag="ACG89C_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4899570.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2967..3773)
                     /locus_tag="ACG89C_001004"
     CDS             complement(2967..3773)
                     /locus_tag="ACG89C_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899571.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3911..5425
                     /locus_tag="ACG89C_001005"
     CDS             3911..5425
                     /locus_tag="ACG89C_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS4899572.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5828..6811)
                     /locus_tag="ACG89C_001006"
     CDS             complement(5828..6811)
                     /locus_tag="ACG89C_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS4899573.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6816..7463)
                     /locus_tag="ACG89C_001007"
     CDS             complement(6816..7463)
                     /locus_tag="ACG89C_001007"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4899574.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7548..8180)
                     /locus_tag="ACG89C_001008"
     CDS             complement(7548..8180)
                     /locus_tag="ACG89C_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4899575.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8196..8747)
                     /gene="coaC"
                     /locus_tag="ACG89C_001009"
     CDS             complement(8196..8747)
                     /gene="coaC"
                     /locus_tag="ACG89C_001009"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS4899576.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8744..9481)
                     /locus_tag="ACG89C_001010"
     CDS             complement(8744..9481)
                     /locus_tag="ACG89C_001010"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS4899577.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9478..10467)
                     /locus_tag="ACG89C_001011"
     CDS             complement(9478..10467)
                     /locus_tag="ACG89C_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4899578.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10725..11267
                     /locus_tag="ACG89C_001012"
     CDS             10725..11267
                     /locus_tag="ACG89C_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4899579.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11284..12369
                     /locus_tag="ACG89C_001013"
     CDS             11284..12369
                     /locus_tag="ACG89C_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899580.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12381..13187
                     /locus_tag="ACG89C_001014"
     CDS             12381..13187
                     /locus_tag="ACG89C_001014"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899581.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13188..14015
                     /locus_tag="ACG89C_001015"
     CDS             13188..14015
                     /locus_tag="ACG89C_001015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899582.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14012..15085
                     /locus_tag="ACG89C_001016"
     CDS             14012..15085
                     /locus_tag="ACG89C_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4899583.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15513..18209
                     /locus_tag="ACG89C_001017"
     CDS             15513..18209
                     /locus_tag="ACG89C_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4899584.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18274..18774
                     /locus_tag="ACG89C_001018"
     CDS             18274..18774
                     /locus_tag="ACG89C_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4899585.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18786..19280
                     /locus_tag="ACG89C_001019"
     CDS             18786..19280
                     /locus_tag="ACG89C_001019"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4899586.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19301..20110
                     /locus_tag="ACG89C_001020"
     CDS             19301..20110
                     /locus_tag="ACG89C_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4899587.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20107..20928
                     /locus_tag="ACG89C_001021"
     CDS             20107..20928
                     /locus_tag="ACG89C_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4899588.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20979..23078
                     /locus_tag="ACG89C_001022"
     CDS             20979..23078
                     /locus_tag="ACG89C_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS4899589.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23075..24193
                     /locus_tag="ACG89C_001023"
     CDS             23075..24193
                     /locus_tag="ACG89C_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4899590.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24232..25110
                     /locus_tag="ACG89C_001024"
     CDS             24232..25110
                     /locus_tag="ACG89C_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4899591.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25256..25453
                     /locus_tag="ACG89C_001025"
     CDS             25256..25453
                     /locus_tag="ACG89C_001025"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4899592.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8063 a         4440 c         5415 g         7609 t
ORIGIN      
        1 gattttattt atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag
       61 tagggaggac taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc
      121 ttgtcgttta gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg
      181 cacgatgctg atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac
      241 acaaaccatc aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat
      301 tactcatact ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa
      361 taaagtcagt tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg
      421 gacatcggaa accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac
      481 aaaaggtgat cagttaaaac agatcactga tattttgaat catgctttga cagatataaa
      541 ggacggtgga agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag
      601 aaagaaaatc gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct
      661 gaagaacgca gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat
      721 cgtggatacg actaatacag gaacatctac tatttccttg tcctattttg atccaattgc
      781 tttaaaaaca atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa
      841 aggaaaagat cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata
      901 tgtaacaaaa agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt
      961 agatactaca actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc
     1021 aaatgtggca gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt
     1081 catcactcat tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag
     1141 tagtgtaaat cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa
     1201 tatagattta tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca
     1261 agcaaacgga ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga
     1321 actgatgaat aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc
     1381 agacaatcgt tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa
     1441 agaagagttg atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt
     1501 atcggttaca atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga
     1561 ttttacgatt atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc
     1621 actattacat gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacgaga
     1681 agaaagcttg tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt
     1741 aaaacaaaca agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa
     1801 aacgataagg caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt
     1861 agagcctaaa ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca
     1921 gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac
     1981 cgatgagcat ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg
     2041 aggtattcct tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata
     2101 cgctaatcga aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat
     2161 agtcaaataa gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa
     2221 aaatgttaga taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa
     2281 atgttccaat cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg
     2341 aatctgcttg ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac
     2401 ttgagtattt tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt
     2461 ttgtagagac aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa
     2521 gagagcaagt gtatccggtt tttcattggt tagcagtgaa accagattac caaggaaaag
     2581 ggctcgcaaa agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg
     2641 gaccaatcta tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa
     2701 tgggttttac ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag
     2761 ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt
     2821 gggataaaaa acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga
     2881 taactaatgg catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg
     2941 ccccttctct tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg
     3001 atgaacgtaa atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca
     3061 actgctccaa taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag
     3121 taaccatttt ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt
     3181 gtccgagtga tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta
     3241 ttgatttgct ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa
     3301 aattccacaa actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct
     3361 agtaataaag gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa
     3421 aagccgacac ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa
     3481 aaatagccat aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac
     3541 aaaatgattt ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg
     3601 atttcaaata aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag
     3661 acctgatcca ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg
     3721 attctacctg cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa
     3781 ctccttcatg tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact
     3841 taaagaaaaa cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag
     3901 ggggatacaa atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat
     3961 gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc
     4021 tgccgaagta tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga
     4081 atcttatgat gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc
     4141 aaaagatgct gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac
     4201 atctaaatca tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat
     4261 caaagactcg aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga
     4321 taaagaaatg cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca
     4381 aaacggtatc aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca
     4441 tcgtccgtct gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt
     4501 ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg
     4561 ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg
     4621 aaaagcaatc gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata
     4681 tcgtcccgat ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat
     4741 caccatgatt gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc
     4801 aactgtgcag caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat
     4861 cttggtcgat caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc
     4921 tgcagcaatt acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg
     4981 tgatcgtgcc acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac
     5041 aatggaagaa ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga
     5101 tggatggaat acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat
     5161 ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta
     5221 cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc
     5281 aattaggggg ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat
     5341 cggtggaaat tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc
     5401 tcgatttgct tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag
     5461 cgttttgagc taataccgag ggtcagctga ttgaacactg tttagtgaag aggttgtgaa
     5521 aaagctgccc tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
     5581 acttggttgg acttattacc gcagatgcaa gcttttgaac accgtttatt cggaaaaaga
     5641 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
     5701 agcagaagaa attccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
     5761 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttgatcag
     5821 catgatttta ttcagttatc ttcaaaatga tttttccgat gtttttgttt gcttccatat
     5881 attcatgcgc ttgtttgact gcgctgaatt ggaatgtacg gtcaatgatt ggccgaatct
     5941 tgccattttc tagaaaaggc ccagcttttt gcataaaagt ttgtgcgagc tctgctttat
     6001 actgatcact tctaggtgtc aataatgtac caagcagttg gacacgtttc ccaatcaata
     6061 caccaaggtc tatctcaggc acgatggtcc cacctagcat accaatcaag acccaacgtc
     6121 catcaacggc tatagaagct aaattctttt cccagtaaga agcgccgatg aaatccaaaa
     6181 taacatcgac tccccgacct tcagtgattt ttcgtacctc ttcttgaaag tcttgttttt
     6241 tatagttgat caattcatct gcaccaagtt ctttacaaaa ggcgagtttt tcttctgatc
     6301 ctgcagtaac gatgattttc gcgtgagaaa gctgtctagc taattggatc gctgctgttc
     6361 ccacaccact tgctcctgca tggatcaaga cagtttcgcc ttcttgtaat tttccaagcc
     6421 agtaaagtgt ttgataagca gtcaaaaaaa cttctgagat tccagcagca gaaacatagt
     6481 ctagcgtatc agggagaagg attgcacgat cggcaggcat cacagcatat tcggcataac
     6541 cacctagatt gactaatccg tatacttttg tcccaggagt aaaagtggag atatcacttt
     6601 tattctcaac tacctctcca gctacttcga ctcccaaaat agggtaggga ggtttcaaag
     6661 agactgattc tcttttcata atatctgttc gattgacagc tgctgtatgg actttgatca
     6721 gtaattcgcc ttgttttggt ttaggaacag gcacttcaat ttgttctaat acatctacag
     6781 aaccaggatg cttgattgaa atggcacgca tggatctatt cctcctttgc ccattcttgg
     6841 aatgctgtat tcaactcttt gctaccttca gaaagtacta gagtcgtacg agaaatatcc
     6901 gtttcttcca ctagagcggc aagtacagca gcaactgtag agcgagtgat cgtttttgat
     6961 gccttctcat taggcttagt taattgaatg gaagtaactt cttcatcgtt agtcaatgtg
     7021 actggttgaa gaatgacaaa atctaagtta gttcgtttca gccattcatc tgcataatgt
     7081 ttagtaatat aataatctgt catggactca ggccattttg ctggttcatc tgcataaaca
     7141 gcactgacca tcagatagcg agagacatgc gcattttcag ctgcgatcac tgttttgatt
     7201 gcgccatcca agtctacttg cagaagattc tttccttgag agccggcagc aaaaatcaat
     7261 acatcgatgc ctttgaatgc ttcggtcatt ttttccacgg accaagttaa atcaaaagag
     7321 acatacgaaa cattctcttt ttttactaat gtttgggaag caacatctct cacccctgca
     7381 aaaatttcat gggaacttgg agctaaatct tttatcaggt gtcggccaat ttgtccgttt
     7441 gcacctacaa caaaaatttt catttggatc atcctttcga cgtcaaatct ttccattcaa
     7501 tagtaacacc aaaagagaaa aaaatcagag atcggctacg atgactatca gttagtaaga
     7561 gaagggtgct tgaagtatct taacaagaca ttgcatactg ctgcagtcgc aagacctgcg
     7621 acgattcctt caggtatacc acttgtaaaa ataaccgcta gaatagctga ataaacagca
     7681 tttaaatttg ttccgatgac tttggcataa tcttgctgga ataaaaagta gatcatattc
     7741 atcactaaaa tcgtattggt caatgaaccg gaaagtccag ctaagaataa agaaacggaa
     7801 tctgcttttt gtttcattag tttgcgcaga cctttataaa cgaaataagg aaccacccca
     7861 atcaagatgc gaggaataaa cgcgatcagc aaggctttcc agcttccatg ttctgttccg
     7921 atgactggga taaacggaga aaagacaaaa gacaaaggtg tttggataac tgtgctacgg
     7981 atcatgctga tcagtccaaa tgtaccgcca agaaaagcac ctagtcttgg tcctaaaacg
     8041 atcgaagcaa tgattacagg aatatgcatg gttgttgcat tgattggacc gatagggata
     8101 aaacctaaag gcgtaacagc taataaaatc aaaatagcta aaaacatcgc agttaatgta
     8161 aagttttttg tatttttcat aagtactcct tttttctaag catttctttc attaagagtc
     8221 agcatgacct gttcgatgat cgtctgaata tcagctaagg caccttttcc aaaatctcca
     8281 caagctaaaa gagattctct tggttcaatt tcctgatagc cgacagatct caatgtttcc
     8341 agattacgct gagtgatcgg gttttgatac atattcgtgt tcatggcagg ggcaatcaat
     8401 tttggtgttc ccactggaag cgccattgcg actgttgaaa gaagatcgtc tgcaatgccg
     8461 tttgctagct tgccgataat gtttgctgta gcaggcgcga tgagaaacaa gtcagcttgt
     8521 tttgctagtt cgatatgatt gattacctgc ggatttgatt cttgcatgac gtctgtatgg
     8581 actgcgcgct tcgacagaga ttgcaaggtt aatggcgtga taaaatgtgt gctgctttta
     8641 gacataatca catctacctg ggcacctaat ttgacgaatt gacttgtaag atcagcgctt
     8701 ttgtaagcag aaatacttcc agtaacgcct agtaagatct ttttcatttt gattcctcca
     8761 gtctaatttt ctcagtaatc aatgcagcaa tctcagattt actttgagct tcttccactg
     8821 ttccatcttt agataagaga tagccgtggt gtgtctcgcc atggatttcc gtcaaatcat
     8881 ttgcgaaaat aaaatcagtt ttgttattta tcagactatt ttttgcgact gccagaagag
     8941 attcctttgg tacatctact aatagtttga aaccaatgat ttttgctttt ggctgccatt
     9001 cacgtagata agaaatgatt tttggtgttt tcttcaaaac gagcacaaga tgctcggtgt
     9061 tagaagagat ctttgaaaca gtggtatctg tctgttcatt ttccgcaaac caattgtcca
     9121 gttcttcttg cgttgaagaa gtgggtaggt tttttgccag ctgttcttct gaaaaactaa
     9181 aagcaggagt aaaatcactg actgccatac tgtggataat cgcatcgtag tgttcttttt
     9241 tcgttagttc ttctaattgt aaaaataagt ccagtgcaga ttcgatcgta taaatcgtta
     9301 tattggagtg tcttttcggt ttcagtgctt gtttcgtagt cacgtaatcg attgtggtat
     9361 ccggattggc agctaaataa tcagctaatg cttgccctaa tcgtcctgtc gaatgatttg
     9421 taatagcacg tacttgatcg attttttcag tcgttcctcc agctgtgatt agaatcttca
     9481 tttatggtca cctacattta ctagtgttct tccttggtgt gttccagcaa gtagtttaga
     9541 tgctgtttca ggcaattgat ccagagtaat ctcttggacg accagttcat ctgcaatctg
     9601 caaatccgcc atctgttgcc ataaagaaag acgttgatct attggtacat tgacagaatc
     9661 aattccaatc aactggatgt ttcgcagaat aaaaggcaag acagtcgcgt ttagtttgat
     9721 tcccccagca ttgccacata aagctactgc accgttataa gagatcaatg gaagaagact
     9781 gcttagctgc tcgccaccta ctgtgtcgat gacatagtcg atttgctgtt ttcctaaagc
     9841 ttttgttgtc tctggcagaa attcatcagg tcgtacgact tgcaaagcac ctagactttt
     9901 tagccatgtg acagactctt cttttctgga aaaggcaatg attgactgga atcctagttt
     9961 atgtaacagg gctatcgctg tactgcctac accgccagaa gcaccagtaa cgacaacttt
    10021 tttatcggag gtcatacctt gacggattaa agcgttcaca cacaacatag cagtaaaccc
    10081 tgctgtacca aggatcatcg cttgacgcag cgtcatattt tttggtagag gaactaacca
    10141 gtctcctggt actttttgat attggctata accgcctgga tgggtgacgc caagcccaaa
    10201 accagtagca agtacaagat cttcttttga aaaacgtgga ttgttacttt ggacgacagt
    10261 tccggataaa tcaatccctg gagtcatcgg atatgttcgt atcacctgac cagactcgct
    10321 cattgccaat gcatctttat aattgacatc agaataggca atttttacca agacttcatt
    10381 ttcagccaac tcagtaaatg gcagatcact gattgctgta tggaaaacag gttcctttat
    10441 tacctgaaaa gatcgaaaag cgttcattcg tgagacactc cttttaaaat tttcttttat
    10501 cataccataa agcctactct tttattcttg ttcttccgta aatttagaca aaaattaggt
    10561 gaaaaataaa gaatcaaaca actagaattt attccaaaag ataaagaaat attcagcaaa
    10621 aaaaatttta caaagggttg cctttttcaa taaatttcgt atactaatac gagtggtagt
    10681 aaacaggaag tttactatta gaaaacttcg attcggagga aattatggaa attggtgaga
    10741 agttaagaaa tttgcgcatc caaaagaatt tgacacaaga agaactaggc gaacgcacag
    10801 atttgtcaaa agggtatatt tcccagttgg aacgagattt aagttcacca tcaatggaaa
    10861 cattcttttc tattcttgaa gtcttaggtg tgactccaga agaatttttt catcaagaag
    10921 cagcgaatct gcaagtggtt tattcaaaag aagatcatac ggtttatttc gatgaagaaa
    10981 acgggtatga attggaatgg ctcgtggctg attcgaatga aaaagaaatg gagcctgtct
    11041 tgctgacttt tgaaggtgca ggagaatata agacgtttga accttcatta tctgaaacgt
    11101 ttatttttgt attagaaggg gaattagagt taagcttagg agagacggtc tatgtggcaa
    11161 aagcaggaca gtcgatctat tatcaagcga caaaacaaca tcaattaagg aatcatctca
    11221 aaaaacgaac aaaagtattg attgttgcaa cgcaatcata tttgtaatat agggggtaaa
    11281 aacgtgggga aaccaatcat ttcatttgat caagtagtga aacgctatga tgatgaaaca
    11341 gtattaaaaa aagtaagttt tgagatcgaa caaggaaaat tttatacact gcttggtcct
    11401 tccggctgtg gtaaaacaac gatcctacgt attatagctg gattcaccga agctacagaa
    11461 ggggacatct attttgaagg gaaacggttg aatgatttgc cagctaataa gcgacaagta
    11521 aatacagtct tccaagatta tgcacttttt cctcatatga atgtttttga taacgttgct
    11581 tttggattga agatcaagaa aatgtccaaa tcagatatcg aaaagaaggt aaaagatgct
    11641 ttgcgtatgg tccagcttcc tggatacgaa aaacgggaga ttagtgaaat gtctggtggt
    11701 caaagacagc gtgtagcgat tgcacgagca attgtaaatg aaccgaaagt attgctatta
    11761 gatgaaccgt tatcggcgct tgatttgaaa ctacgtacag atatgcagta tgaactgcgg
    11821 gatctgcaac aacgattagg aatcacgttt atttttgtta ctcatgacca agaagaagcg
    11881 ttggcaatga gtgatgagat ttttgtcatg aataaaggga aaatcgtcca aagtggtaca
    11941 cccgtagata tttatgatga gccaatcaat catttcgttg ctgattttgt tggggagagc
    12001 aatatcgtgg acggtgtaat gatcgaagat aatctagttg agtttgttgg taagcaattt
    12061 gaatgtgtcg atggcgggat gcgtctaaat gaaccagttg aagttgtttt acgcccagag
    12121 gatttgacga tcacgacacc agataaagga aaactagtcg taacggtcga tactcagctg
    12181 tttcgtggtg tccattatga aatcatctgt tatgatgaac agcaaaatga atggatggtc
    12241 cattcaacga aaaaagctaa agaaggaagc aaagtaggat tggcctttga gccagaagat
    12301 atccatgtca tgcgtttcaa cgagtctgaa gaagaattcg atgcacgtct tgacagctac
    12361 gaagaatagg ggggacaaga atgaaaacaa tgcgcaggat ctattccgtc ccttatattt
    12421 tgtggctcgg tttattcgtg atcgcgcctg tattaatgat catttatcaa tctttttttg
    12481 atatgaatgg gcaattcacg ctgaataatt atctatctta ttttacgtcg gggacatcgc
    12541 tttctatgac gatcaattct gtatggtatg catttttgat cacgtttttc acgctgatca
    12601 tcagttaccc gactgcttat tttttaacaa aactgaagca caagcaatta tggctgatgc
    12661 tgattatttt gcctacatgg gtgaatctgc tgcttaaagc ttacgcattt attgggattt
    12721 tcagtgtgca cggcagcgtg aatcatttct ttgattttat cgggatcggg ccgaaacaga
    12781 tattattcac agacttcagt tttctgttcg tggcaacgta tatcgagatt ccatttatga
    12841 ttatgccaat tttcaatgca ttggaagaga tgaatccttc tttgatcagt gctagtagag
    12901 atttaggcgc gaatagcttt gagaccttcc gacgagtgat ttttccgtta tcattaaatg
    12961 gtgtgaaaag tggtgtccaa gcagtattta ttccttctct ttctttattc atgctgactc
    13021 gtctgattgg cggaaatcgg gtgatcactt taggaacggc tatcgaacag catttcttgg
    13081 ttacacaaaa ctgggggatg ggttctacaa tcggtgtgat tttaatcatt gctatgttca
    13141 tcgtgatgct cctgacaggc gagaagaaaa aaggaggtcg ctcataaatg aaaaaattta
    13201 aatggtcgta tctttattta gggatcgtct ttttattctt atatgcaccg atcttctatc
    13261 tgatttttta ttccttcaat gaaggaggaa cgatgaacag ctttacagga tttacttggg
    13321 aaaattatga agctgttttt gaagatacac gtttgatcat tatcgtattg aatacgttca
    13381 tgctggcttt cttatctgca ttgatcgcca cgatcatcgg gacatttggg gctatgggaa
    13441 tctattacac gaaaaaacga cgtgcacgga cagcgttgtt gagcatgaat aacatcttat
    13501 tagtctctcc tgatgttatt atcggtgcta gtttcttgat tttctttaca ctactaggat
    13561 tcggattagg ttttcagagt gtattgcttt cccatattgc tttcagcatt ccaatcgtcg
    13621 tcttgatggt cttgccaaaa ttgcaggaaa tgaacgattc aatggtcgat gcggctagag
    13681 accttggtgc taataatttc caagtagtca aaaatatcat tttgccttat ctttctccag
    13741 gaatcatagc tggatatttt atggcattta cctattctct tgatgatttt gcggttacat
    13801 ttttcgtcac aggaaatggc tttacgacgc tctcagtaga gatctattct cgtgcaagac
    13861 aaggaatcag tttagaaatc aatgcgctaa gtgcgttagt tttcttgttc tccatgatat
    13921 tggtcattgg atactacttt attagtaaag aaaaccgtcc aaaacggctg aagaaaaaca
    13981 gaagagtacg cgagggggtg gcacggctgc gatgaaaaaa ttacaatctc tgtttttagg
    14041 tattctcatc attattgcgg tgctgctgct tggtgttcgt caacttgaaa aaacaagtgg
    14101 catgagtgga gcaaaggtac ttaccatcta taactgggga gattatatcg atcctagttt
    14161 gatcacgaaa tttgaaaaag aatatggata caaagtgaat tatgaaacat ttgattctaa
    14221 tgaagcgatg ttcacaaaaa tccagcaagg tgggacaaac tacgacatcg ctataccaag
    14281 tgaatatatg atccaaaaaa tgatcaaaga aaaactagta cttccattag atcattcgaa
    14341 gatcaaagga ttaaataata ttgatccacg atttttagac ttggattttg atccagataa
    14401 tcagtattcg attccatatt tttggggaac tttagggatc gtatataacg ataaattctt
    14461 tgatgcaaat caaattaagc attgggatga tttgtggaag ccggaattaa aagacagttt
    14521 gatgctgatt gacggtgcac gagaagttat ggggctttct ttgaatagtt taggctattc
    14581 tttgaatagt aagaatatgg cacagttgac agaagcagca aataaattga atcatttaac
    14641 accaaatgtc aaagcaatcg tggcagatga gatcaaaatg tatatgatca atgaagaagc
    14701 ctccgtggca gtgacatttt ctggcgaagc tgccgatatg atgtgggaaa atgaacattt
    14761 acattatgtg atcccaccag aaggatccaa tctttggttt gataatatcg ttatgccaaa
    14821 aaccgtgaaa aataaagaag gtgcctatga tttcatcaac tttatgctag aaccggaaaa
    14881 tgcggcacaa aatgcagagt atattggtta ttctacacca aatgaagaag caaaagcgtt
    14941 attgccaaaa gacatttcgt cggacgaaca attctatcca tctgatgaca cgatctccca
    15001 tcttgaagtc tacaaagatc taggatcgaa atacctaggc atctataatg acctattctt
    15061 agaattcaag atgtatagga gataatggca aaactcagta agaatgggga cttatcaacg
    15121 ttctaaagag ctaaaaatcg tcctttttct atctgagttt tccaatcttc taccaaaagc
    15181 ggatgaaacg ccgctttttt tgtgttattt tattttttct accacgtttt tctaccagag
    15241 aaaagtggac aaaaccattg taaaataggg cccgagacgg gctagtaaat ctaagattga
    15301 aaaagttaca gaaggataaa gtaattggaa actatgcaaa tatacttaac ccttctgtaa
    15361 tctattaata aaactaatat atgaatccag ttcaatattt gtagctatga aaaaacataa
    15421 aaatcatatt agataaaaac taaattggta gataaattat tttgtcagat atctgaggca
    15481 aatattcgac tatttgtgtt tcaaagtaaa aattggaata caaatataga atagacatat
    15541 tgcagccgat agtttattat gcttttagac acattaaact ggaggaaaaa atgaaaaaga
    15601 gcgctaaaga tcgagttttt gatgagctga tacgactaac agggaatatg tcttctgtca
    15661 ctgccgagaa aatttcgaag aatctaggta tctctcgtca gaatgccagc cattatttga
    15721 cgagattagt ggaagataaa aaggtcgaga aattaccggg aaagcctgtt ttgtggaagc
    15781 ctttagatga atatgcggtt gtagataata cggagcaaat aaacgaagcc tttcattctg
    15841 tagttggtca tgacggaagt ctgagagagg tcattcaaaa atgtatagcc gctgtcaaat
    15901 atccgcctaa cggactcagt gtattaataa atggagccac gggtgttgga aaaagttttt
    15961 tagccacaaa aatttttgaa tacgctatcc atgagcaaat tattgaaaaa gatgcaccat
    16021 ttgctatctt aaactgtgcc gattatgcgg acaatcccga actgctttcg gcaacactgt
    16081 ttggatacaa gaagggggcc tttacggggg ctgaaaagga taccgatggt ttattggcaa
    16141 cggccaacaa cggctatctt tttctcgatg aggtccatcg cctatcgaaa gaaaatcaag
    16201 aaaagctctt tctatttatt gatactggaa actatcgtcc agtcggtgaa aatgtaaatt
    16261 ggcattctgc taaggttcgt tttattttcg ccacgacgga aaaaggtgaa aattatttat
    16321 tagacacctt tgatcggcgt attcaaatta gtgttatgtt gccaactttt gaagatcgcc
    16381 ccattcgaga acgattagag ctgattcaat tattttttca aaatgaagcg gatgttttac
    16441 aaaaagatat tattgtttca agagaagcct tgtctttaat gttatctcat ccctttagtg
    16501 ggaatattgg taagctgaag aatatcatta aaatcagttg tgcggatgtg tatagtcgaa
    16561 cacaagaaga accattaacg attgggaaaa atgagatctt gattcagtta aatatgctgg
    16621 aatccgaagt agaaagcttg ccgattgcta gtttattgat aaagaaaaat caaaagataa
    16681 aaacggatcg aatcaaaccg tttgattatg attcgatttt ggaaaaaatt actcagctga
    16741 taaatgaagg taatttcaat gaaaattttt cttcgataaa gaatgaagta cagcatttgg
    16801 tgcatcacat tgataaggat tcttttttat caatgagtag aaaattgatg attcagatgt
    16861 ttagaaaggt ctgggtatcc gttttaggaa aaacgtacgg attgacgacg agtggacctg
    16921 ttgcagaaat tattagcaga tcctatgtca tttccccaag ctataagaca gacttaaccg
    16981 ggataaatcg tacattagct gcaaatctac ctcggacgac ttacttatgt aatcagttct
    17041 tggagcattt accaccgctc tcagaaaagg acgagaactt tctaagactg cttattgtcg
    17101 tgactttatc tgattatgta gatgaatcca tcgagctgaa gggcctgttg cttgctcacg
    17161 gagatagtac ggctagcagc attcaagcag tagtgaatca attatgcggc aattatgttt
    17221 ttgaagcgtt ggatatgcca gtagagagta atgtgtcaga gattgtcgaa tcgacaagga
    17281 atttcgtgaa acggcaagtg aatacagata gtctagtgct gatcgtagat atgggctctt
    17341 taaatcaaat ttacacacag atcaagaacg atttaaaagg ggagttactt cttctgaata
    17401 atttgacgac ttccattgca cttgatattg gcttgaagat gacgatgaat gcgccattta
    17461 aacagattgc ggaggatgca gcagacaagt attcaatcaa tgttcaatac tttgaaggct
    17521 tttctcaaag tagcaacatt atcatttcct gtatgtcggg tttggggatt tcggacaaat
    17581 tgaaagatgt ttttcgtcat catttatcgg atgaatccat cgaagttttc acgaaggact
    17641 acagagaact acgtgaatta atcgaccaaa atgatgaaac ttattttgac aagacaaaat
    17701 tggtcatcac gacttcggat cttccacgtt ccttttcaat tcctaatatt aatgtttatg
    17761 acatcttaga cggtgatgga gcagccaatt tggagcatat tttgagtcct gaaatgaagc
    17821 cggcagaatt taaccgcttg attcaggagc tagtaaaatt cttttcgttg gaaggaattg
    17881 cggaccgttt gaactttctg aacccaacga tcgtgatgag tgaagtggaa acagtcattt
    17941 ctaaatatga aaattactat catttcactt taaatggccg tgtaaaacta aatctctata
    18001 tgcatacggc attgatgatg gaacgtttat tcttggctcg gacagaaaaa tctgaagaac
    18061 cgaattatga tttgaaacca gatgaagatg aattttatta tgtttctaga agtatttttc
    18121 aatcaatgga gatgaaatat aattttaaag tcaatgctta tgagctttcg ctgctctatg
    18181 aattattagg accgtatatt caaaaataaa gtgtctaaaa aagtgtctaa tctgtttttt
    18241 ttaggttaga cacttttttt aatgtgaatt aacgtgatta aaccaatgtt tttaaaagtt
    18301 ggcacaggaa ttgcttatat aaaagtgaac agaaagaaag gaattaacca tatgaagaaa
    18361 attctaatag cgactcacgg tcacttagct tcaggctttc ttagcagtat tcaattactg
    18421 acggggaagg ttcaagagat tacggtgatt aatgcttatg tggatgagtg tgattttgaa
    18481 caggagttga atgtatttgt aaaccaggtg aacgagcagg atcagatttt tgtttttacc
    18541 gatttatttg gcggaagtgt caatcagaag attaccaaaa cctttttgga gaaagagatt
    18601 gctgtaacta ttttgtctgg ctttaatctg ccaattctgc tcgaaatagt attggcaacc
    18661 aaggaattaa caaaggagga tattcaggaa ctggtaaata aatgccaaca ggaattaaag
    18721 atgaccattc ttgatgaggt acatgtagta aatgaagagg aagatttttt ttaggaggaa
    18781 gaaaaatgat taaagcagtg cgaattgatg aacgattaat tcatggacaa gtagcgatga
    18841 cttggacaaa gagtctaaat ttaaccgggt tagtagtagc aagcgatgag gcggctacca
    18901 acgatattca aaagatgaca ttgaagatgg ctgcaccatc gaatgtcaaa gtcattatta
    18961 aaacagttga tgacgcgatt catttgttaa aagatccgcg ggcgaaggac atgcgcttga
    19021 tggttttagt ccctaatgtc aaagatgcgg taaaagttgc gaaagctttc ccaacggaaa
    19081 tcgaattaat gaacgtgggg aatgctggga aaatgacacc gccatcggaa gataagaaaa
    19141 ccttatcaaa ggaagtgatg ttaacgacgt cagaaattca agacttaaaa gaattagttg
    19201 aactttatcc agataccgtt tttcagccta caccggcgat ggaaaaacgt tcagctatct
    19261 ctattttgag tgatttttag aaaaaggaag gagaaaaatt atgtatgatg catttgtagt
    19321 cgctttggcc gtttttattg gaattgctgg acatgaaatg ttcgggatgt ccatgctgag
    19381 tcgtccgatt gttgttgctc cgttagcagg agcgttgatg ggggatttgc agacggggtt
    19441 gattgtcggg gcgtcactag aagccatctt tatgggtgtc gtcaatatcg gaatctctag
    19501 tacggcggag ccttctttgg ctgctgggct ggctacagcg ttcgcaatca aaatgggagg
    19561 aaacgtggat gcgattattc ccattgtctt tccactagca gtattaggat tacagttaat
    19621 gaatatgatt ttttcttttg tatgtggacc gatggctgct aaatttttca aatttgcaaa
    19681 atctggtgat caaaaaggaa tcatcaatct gcacttcttt atgtggtttg tccactatgc
    19741 gctttatgct cttattccat tctttgccgt tttatttgga gcagatgttg tcaaaagcgt
    19801 cttggataat attccagagg tcatcatgaa cggattaaca gtcgctggaa atctgctgcc
    19861 ggcagttggg atggcgatgc tgttacggat gctttggagt aaaaatattg ctgtgtggtt
    19921 tttctttggg gccgttgtta tggcgtatct cgagttacca ttgattgcaa ttgccgcagt
    19981 tggaacgatt attgcaattg ttatagccca aagagatcta caactaaaaa atatatctgt
    20041 tcatgcagtt caaagcgatg ctgctgtaaa tgcaacgctt gaccaagaag aggaggattt
    20101 ctttcaatga aattagctga tggaatttct aaagatgaga aaaaattggt tcggaagatg
    20161 ttttggcgct cgatgactat gtatgcttcg gtgaatccgg tgacgatggg cggcggtggt
    20221 ttcgcctatt ctatgatgcc cttcattaat catttctaca aaagtgaaga agatcgagct
    20281 gcggcgctgg aacgtcatac acagtatttt tcgacgacca ttccttgcgc gtcctttgtc
    20341 atggggattg cggcatcgat ggagaaggaa aattctgaaa aagctggttt tgacgtggaa
    20401 agtatcaacg cgatcaaaac gagtttgatg ggaccattgg ctggaattgg cgattcattg
    20461 ttctggggag tttggcgtgt cgttaccgcg ggactagcga tcaacttagc caatgccgga
    20521 aatattttgg caccaattat ctttttagtt atgttcaata tacctaatta cttgatgcga
    20581 tactatggcg gcgttttagg ctattcatta ggctctaaat atattgaaaa gctgtatgcg
    20641 aacgggttaa ttgaaattct gacaaaagct gctagtatct tgggtctgat catggtaggt
    20701 gcaatgacga tcagtatggt caaattcaat accgttttga gcttttcaat gaagggcgaa
    20761 gaactaatga atgtccaaga aatcctcgat agtattttta aaggaattgt accgttattg
    20821 gtaacattga gctgtttcaa attactagat aaaaaagtcg gcgtcatcaa aattattttt
    20881 ggaatggtcg tcttgggaat tttattgtca ttacttggaa tcgcatagta ggaagctgca
    20941 gctactaggc tgcagttttt ttagcaagga gggaaaagat gactattaat gaagtaattt
    21001 attcacccaa acacccattt tttaatgctg agttaaacga cggaaatgtg actacttcaa
    21061 acgaatacag ctactatcgg aatattcctg atgtaaaggg attgaccttc gatgtaagta
    21121 atcaaaagac attagcaaac cttgatgttt gcgggacgat tcaaacgttg accttttatc
    21181 gagaaaattt actaactgag gaaaaaccag gtgtttgggt caataaacag ctctctcaaa
    21241 ccaattcgtt atccttaaag gttgaagtaa acgggattgt ctaccatttg tcagaatcgg
    21301 atcatcgtat tgaagtcgat gtgattcaag actgtttgcc tagatatatt catcattatt
    21361 cgacgtttaa ggtcattgta gtatcctttg ctcccatact ggctaataag cgtttaagta
    21421 tgcttgttca acaggtttac attgtgaatg aaacacacga aagccttcaa gtttcagtga
    21481 aagatgtgcc ttcgtatcaa gagaagtatt cggatcagca aaatgtgttg atttctcaaa
    21541 aaggaaataa ggacacgatc gcacaacagg aagtggcaag cttcgccatt gcttttgtgg
    21601 acccaaatgc ttttgaagaa gtaatgactt ttcaggaatc tgatatcgaa aaatggctga
    21661 aggaaacatt gaactatttt gctcagctat ttgggcagct gtcaatgcca aacgatagag
    21721 ttgtccatct ctatcgtcgt gcactttatc aaagcttttc tagtttcggt atgaatcgac
    21781 agggacaggt cgtcggcagc aattggggca gctatcctgc tactaatcga atttggaata
    21841 aggatatgta ttacgcatca ttgccattca ccatgtttga accggagtta tgtcaaaaaa
    21901 cggttctatg gtttgatcaa tatggcgtta agtttcctgg ttcaaaattt acaggaggta
    21961 tcaatcattc tctgagtaat tcgttatcca gtgcgttact ggctagtctt tattttgaac
    22021 atactgggga tacaaaattc tttgagcggt gtccgcaggt tctactgaat gcttcaagaa
    22081 ttattgagga catattagct caacgtcggt caggggaacc aatgcttttt ccatctgttt
    22141 ggttatcaga cgcctttgca cttggaaagt atcataccgg cagcaatctt tgtctttgga
    22201 aggcctgtat tggacttgca gaaatttttg aagccctaga acaactgagt cttagcaagc
    22261 gatacaaaaa cattgcctgt aaactaaagg aatcaatttg taaacaaatg actatcgatg
    22321 gtccttttgg tgagcaattt ttggagggaa tcggtgatga ggagaaaaca acctattcag
    22381 tgaagcacta tcaaaaaccg attcttgagc aagggttaat ctttttaagt gatgtgattg
    22441 tggatggaaa aattaattta ttgatgcatg atggagaaga atcagacact acgttgatgc
    22501 ctttttatca atttttagat aataaagatc atttatatca gaacaccatg gccttttctg
    22561 ctagttcttt caatccaacc tatagcgagg aaatcaaagg catcacttgg ggattagaat
    22621 ccggcgccac ttttcctggc tttatcactg ttttaatgag cgagctgcac aaccctcaag
    22681 ctttcgtaac ccgattagaa gaactaattt gtttggcaga tttagacggt tcgtggtggt
    22741 ggtggccgta tcgcttagag gctcaacaag gagaagtcgt gcgagacttt ggctgcggca
    22801 aatgcggctg ggcatcgggt ctttttgtca gcctaatgat cagtcaatat ttcggattga
    22861 aattcaaaaa aggagtattg caaattgatc ctgttgataa tttaacgttt tcttggaaga
    22921 atttgaagtt cggtcaagcc ttcatttcga ttgaatgtac gcaatccgag ttgagtattt
    22981 caaatttgtc agaaaagcca ttgaaaataa gtgatacgaa aaaaattctt cacgtagaaa
    23041 agggaaaaac gattcacatt caaaggagaa aaagatgagt atactaaaaa atattaccca
    23101 atttaaaaaa atccagagtc gagcaatcac tcccgaaaat ccaacaggcg agaaaggtaa
    23161 agctgcgatg ttgccaagcg agctaggacc aagccgcaaa ggtcgtggcg caattccgct
    23221 gacttctggt gaagaaactg tgattgcgga tattactgga acgggaataa ttcgtcatat
    23281 gtggatgacg attcgtgatc gaacagaatt tggcagtttt gttttacgtg atttaatctt
    23341 aagaatttat tgggatgggt cggatagccc tgctgttgaa agtcctttag gggatttctt
    23401 ttgtaatggg tttggagagc gttgcgaggt gaactcgttg ccgatagtgg ttaatcctac
    23461 agggggcttt aattcctatt tcgaaatgcc tttccaaaca tccgctaaaa ttacgatcga
    23521 gaatttacat ccgaaggata tgtcctcatt tttttatacg ataaattacg ctttggtgga
    23581 cgaattggaa gaaaatagcc tatattttca cgcaaattgg catcgggaac gcattaccaa
    23641 attacaggag gattataagt taattgatcg attaaagggt caaggctatt acgtgggtac
    23701 ttaccttgcg cttacctgcc tagaacgtta ttggtggggc gaaggcgagt ttaaattcta
    23761 cattgatggg gatagtgagt atccgaccgt aacctcaaca ggttcagaag attattttgg
    23821 cggggcgtgg gcctttcatg agtacgatga gttcggacgt ccacatgcga aaaactattc
    23881 aacagccttt ttagggtatc cttaccaatc aaatagagat gccacaagag atcgttttga
    23941 aacgggaaaa ttgaatgccg ttcatgcctt tggtgacgac gcaatgccac gacatggtct
    24001 ttatcgttgg catcttcaag accccatctg ttttaccgaa gatttatcgg tcactttaca
    24061 acaaatcggc aacgatgatg ttcgcttatt tgagcggcaa gacgatgttg ctagtgtcgc
    24121 ttactggtat tcaacagaag cttcaggaaa tactaaacgt catttcactg atcgagaacg
    24181 cttgccgcgt taatgaagtg tttttcacat attcgaaggg aggaacctat gatgatatat
    24241 ggggcaattg aagctggagg gactaaattt gtctgtgcag tcggtactga ggaattaacg
    24301 attttggaaa gagtcagttt tcccacaaca actccagttg aaacaatgaa gcaggtcatt
    24361 gattttttta aaccttttaa ggaacaattg gcaggcatcg gggtcggctg ctttggtcca
    24421 attgatattc aatgcaaatc aacgagtttc ggtcatatca catcaacacc aaaacttgct
    24481 tggcaaaatt tcaattttgt aggaacgcta aagcaatatt tcaatattcc gattgaatgg
    24541 acaacagatg tcaatgcggc ttgctatggt gaatatgttg ctggttctgg caaagggtta
    24601 gcaggtgttg catacttcac tgtgggaaca ggtatcggag gtggtgcctt gttcaatggg
    24661 actttcgtgg aagggttcag tcatccggaa atggggcata tgttgatcaa gcgacatcca
    24721 aaggacaatt tttcaggaaa ctgtcccttc caccaagatt gtttagaggg gatggcagca
    24781 ggaccagcga ttgaaaaacg gttgggtgta aaaggccaga atttattggc tgacgattcg
    24841 ttttggcaga ttgaagcctt ttaccttgcc caatgtgcat acaatacaac actgatgttt
    24901 tcgccagata gaattatttt tggcggtggt gtaatgaaac aagaacacat gaagaaaaaa
    24961 gttcaggata aatttgttga gcttataaat ggatatgttg aaataccacc aattgatagc
    25021 tacattatta cgcccgaatt aggtgataat gccggaataa tcggcggatt agcattggcc
    25081 agaaaagcgg taagaaataa gcaaccataa ttaatgaatg tttcattgat actcatgtca
    25141 aaagtattta accgagtaat caaatgatca aatgaagttg gataaagcga aaatgaattc
    25201 aaataaaaag gctctaagtc aaatcgtgtt gttttcatta aatcactgaa aggaaatgct
    25261 tctgcgtttc cttttttctt tttgttgtgc gctgtatacc ttgaggatat tcactggtgg
    25321 cgtacactta aatagtaata tattgtgtta ctttatgtct gtggtaatag ttatagttgc
    25381 ttaccaatgt atgttcattt ttcgaaatgt tgacttcata atccttggct taatgtttga
    25441 atttttttgg tgatagattt tttcagccgt ataaaataaa tcgtaattct attatagaga
    25501 gttgtgatta taaaaagata gcaagaa
//
LOCUS       DAZUKR010000028        24607 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000028 DAZUKR010000000
VERSION     DAZUKR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.028"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG89C_001026"
     tRNA            8..81
                     /locus_tag="ACG89C_001026"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACG89C_001027"
     tRNA            101..173
                     /locus_tag="ACG89C_001027"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACG89C_001028"
     tRNA            201..274
                     /locus_tag="ACG89C_001028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACG89C_001029"
     tRNA            288..361
                     /locus_tag="ACG89C_001029"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACG89C_001030"
     tRNA            385..474
                     /locus_tag="ACG89C_001030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACG89C_001031"
     tRNA            487..560
                     /locus_tag="ACG89C_001031"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACG89C_001032"
     tRNA            579..654
                     /locus_tag="ACG89C_001032"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACG89C_001033"
     tRNA            656..726
                     /locus_tag="ACG89C_001033"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACG89C_001034"
     tRNA            740..813
                     /locus_tag="ACG89C_001034"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACG89C_001035"
     tRNA            838..925
                     /locus_tag="ACG89C_001035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACG89C_001036"
     tRNA            947..1018
                     /locus_tag="ACG89C_001036"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACG89C_001037"
     tRNA            1041..1130
                     /locus_tag="ACG89C_001037"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACG89C_001038"
     tRNA            1143..1216
                     /locus_tag="ACG89C_001038"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACG89C_001039"
     tRNA            1235..1307
                     /locus_tag="ACG89C_001039"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACG89C_001040"
     tRNA            1311..1383
                     /locus_tag="ACG89C_001040"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACG89C_001041"
     tRNA            1388..1458
                     /locus_tag="ACG89C_001041"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACG89C_001042"
     tRNA            1471..1544
                     /locus_tag="ACG89C_001042"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACG89C_001043"
     tRNA            1556..1627
                     /locus_tag="ACG89C_001043"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACG89C_001044"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACG89C_001044"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4899593.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACG89C_001045"
     CDS             3216..4688
                     /locus_tag="ACG89C_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4899594.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACG89C_001046"
     CDS             4771..5559
                     /locus_tag="ACG89C_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4899595.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACG89C_001047"
     CDS             complement(5626..6294)
                     /locus_tag="ACG89C_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS4899596.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACG89C_001048"
     CDS             6409..8121
                     /locus_tag="ACG89C_001048"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899597.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="ACG89C_001049"
     CDS             8124..9893
                     /locus_tag="ACG89C_001049"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899598.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="ACG89C_001050"
     CDS             complement(10098..10664)
                     /locus_tag="ACG89C_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4899599.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="ACG89C_001051"
     CDS             complement(10664..11620)
                     /locus_tag="ACG89C_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4899600.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="ACG89C_001052"
     CDS             complement(11620..12276)
                     /locus_tag="ACG89C_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4899601.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="ACG89C_001053"
     CDS             12646..14664
                     /locus_tag="ACG89C_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4899602.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    14734..14940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15091..17505
                     /gene="leuS"
                     /locus_tag="ACG89C_001054"
     CDS             15091..17505
                     /gene="leuS"
                     /locus_tag="ACG89C_001054"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4899603.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="ACG89C_001055"
     CDS             17853..19406
                     /locus_tag="ACG89C_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS4899604.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="ACG89C_001056"
     CDS             complement(19787..21571)
                     /locus_tag="ACG89C_001056"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899605.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="ACG89C_001057"
     CDS             complement(21586..23307)
                     /locus_tag="ACG89C_001057"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899606.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="ACG89C_001058"
     CDS             23407..24168
                     /locus_tag="ACG89C_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4899607.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7669 a         4489 c         5190 g         7259 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctac
//
LOCUS       DAZUKR010000029        24444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000029 DAZUKR010000000
VERSION     DAZUKR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.029"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..956)
                     /locus_tag="ACG89C_001059"
     CDS             complement(108..956)
                     /locus_tag="ACG89C_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4899608.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1180..3120)
                     /gene="thrS"
                     /locus_tag="ACG89C_001060"
     CDS             complement(1180..3120)
                     /gene="thrS"
                     /locus_tag="ACG89C_001060"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4899609.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3457..3636)
                     /locus_tag="ACG89C_001061"
     CDS             complement(3457..3636)
                     /locus_tag="ACG89C_001061"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS4899610.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3683..5506)
                     /locus_tag="ACG89C_001062"
     CDS             complement(3683..5506)
                     /locus_tag="ACG89C_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4899611.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5784..8063
                     /gene="helD"
                     /locus_tag="ACG89C_001063"
     CDS             5784..8063
                     /gene="helD"
                     /locus_tag="ACG89C_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS4899612.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8156..8803)
                     /locus_tag="ACG89C_001064"
     CDS             complement(8156..8803)
                     /locus_tag="ACG89C_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899613.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8982..9701)
                     /locus_tag="ACG89C_001065"
     CDS             complement(8982..9701)
                     /locus_tag="ACG89C_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4899614.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9832..10983)
                     /locus_tag="ACG89C_001066"
     CDS             complement(9832..10983)
                     /locus_tag="ACG89C_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS4899615.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11200..11940)
                     /locus_tag="ACG89C_001067"
     CDS             complement(11200..11940)
                     /locus_tag="ACG89C_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4899616.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11944..12267)
                     /gene="rsfS"
                     /locus_tag="ACG89C_001068"
     CDS             complement(11944..12267)
                     /gene="rsfS"
                     /locus_tag="ACG89C_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4899617.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12324..12917)
                     /gene="yqeK"
                     /locus_tag="ACG89C_001069"
     CDS             complement(12324..12917)
                     /gene="yqeK"
                     /locus_tag="ACG89C_001069"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS4899618.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12901..13551)
                     /locus_tag="ACG89C_001070"
     CDS             complement(12901..13551)
                     /locus_tag="ACG89C_001070"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4899619.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13561..13872)
                     /gene="yhbY"
                     /locus_tag="ACG89C_001071"
     CDS             complement(13561..13872)
                     /gene="yhbY"
                     /locus_tag="ACG89C_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4899620.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13888..14997)
                     /gene="yqeH"
                     /locus_tag="ACG89C_001072"
     CDS             complement(13888..14997)
                     /gene="yqeH"
                     /locus_tag="ACG89C_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS4899621.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14994..15521)
                     /locus_tag="ACG89C_001073"
     CDS             complement(14994..15521)
                     /locus_tag="ACG89C_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS4899622.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15701..16486)
                     /locus_tag="ACG89C_001074"
     CDS             complement(15701..16486)
                     /locus_tag="ACG89C_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4899623.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16479..17348)
                     /gene="accD"
                     /locus_tag="ACG89C_001075"
     CDS             complement(16479..17348)
                     /gene="accD"
                     /locus_tag="ACG89C_001075"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4899624.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17349..18728)
                     /locus_tag="ACG89C_001076"
     CDS             complement(17349..18728)
                     /locus_tag="ACG89C_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4899625.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18736..19155)
                     /gene="fabZ"
                     /locus_tag="ACG89C_001077"
     CDS             complement(18736..19155)
                     /gene="fabZ"
                     /locus_tag="ACG89C_001077"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4899626.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19180..19656)
                     /gene="accB"
                     /locus_tag="ACG89C_001078"
     CDS             complement(19180..19656)
                     /gene="accB"
                     /locus_tag="ACG89C_001078"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4899627.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19659..20894)
                     /gene="fabF"
                     /locus_tag="ACG89C_001079"
     CDS             complement(19659..20894)
                     /gene="fabF"
                     /locus_tag="ACG89C_001079"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4899628.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20913..21650)
                     /gene="fabG"
                     /locus_tag="ACG89C_001080"
     CDS             complement(20913..21650)
                     /gene="fabG"
                     /locus_tag="ACG89C_001080"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS4899629.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21662..22579)
                     /gene="fabD"
                     /locus_tag="ACG89C_001081"
     CDS             complement(21662..22579)
                     /gene="fabD"
                     /locus_tag="ACG89C_001081"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4899630.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22686..22910)
                     /locus_tag="ACG89C_001082"
     CDS             complement(22686..22910)
                     /locus_tag="ACG89C_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4899631.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22963..23925)
                     /locus_tag="ACG89C_001083"
     CDS             complement(22963..23925)
                     /locus_tag="ACG89C_001083"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4899632.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23935..24375)
                     /locus_tag="ACG89C_001084"
     CDS             complement(23935..24375)
                     /locus_tag="ACG89C_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4899633.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6687 a         5435 c         4258 g         8064 t
ORIGIN      
        1 ttgaaaatta tgttttctta agtcgtgaaa cactttgatg gatcatttat ttgtggaaaa
       61 ctacgttttg ttattctaaa aaacttatat aaaaaatagt aaggttttta tttcgaatcc
      121 ataaaatatt cttggattgc ttctcgtgtt tttttcactt tttcgctaag cttttcgttt
      181 ttagccatat agaaataaaa aatgatatcg atcgtagcaa attgggccaa aagtgaatta
      241 gttgccgcac ttcgattcgt gatttctttt ggtctagatg tttgtacgag tacatcggct
      301 atcttgctaa gcgggttatt tccaaattgc gtcaacgcaa tgatttcgat attcatgctt
      361 ttcgccagct cggctaatgc gactacatct gcacttctac ctgagtttga aacaagccaa
      421 agcactttct ttttctcatt gctgacaagt tgcgggagca agacgtggat atctttttca
      481 aaaatgattg gcttaccgac acgagaccat ttttgcagaa tatcttctac tactaaagaa
      541 gaggcgccaa ccccatataa aaaaacagtc tctgccgatt ccaaacactc tacgactttt
      601 tggatcgttt cttcttctag aatatctact gtttctttta tcgctacttg tgcattgtta
      661 ccaagtttgt ttttgataga gtaaaaggac tcatttgctt ctatatcact aaaaccttca
      721 tcgattccag tctgtagttc tgttgaaatc gtgattttga agtccgtcag actttgaaaa
      781 ccgatttttt tggaaaaacg tacaactgtc ggcgcactca acccggaagc ttctgctaat
      841 tcattagctg tcattgtagg gaccttctca atattaccga taatgtagtt ggccaacttt
      901 ttttctgctg aagaataatc ttccatcata ctttcaatac gactaattac gctcataagt
      961 tcctccaccc ttttaatgat agtctatgta agtataccat atcagaccct tattcacggt
     1021 cgtatactgg ataaaaaaca aaaaaacaat ccgcccaaca gaatatctta tcccatcaat
     1081 cccacagatt ctattgagca tctgtgtctt tgaggaatac ctgttatcta ttgttcggat
     1141 tgctttctat ctactacttt tttacaggaa atttacctat tagttttctc tgctgtaatt
     1201 tttgacttct gcttccatag catcaataaa gatattcaaa tcttctacag ctgtttcttt
     1261 acttccataa cgacgtacgt tcactgttgc atcttccaat tccttgtctc caacaactaa
     1321 ttggtaaggg atcttttgtg tttgagaggc acgtatctta tagcccattt tttcattacg
     1381 atcatcaact tctacacgca accccttcat ttgcaaacgt tctttgattt cataagcata
     1441 atcgccatgt gcatcaacag aaacagggat gattgttgct tgtataggtg ccaaccatgt
     1501 tggaaaagcg cctttgtata cttctgttaa ataagcgaca aaacgttcca ttgtagaaac
     1561 aattccgcgg tggataacta ctggacgatg cgtattttcc ccgtcttctc caacatatgt
     1621 caaatcaaag cgctctggca ataagaagtc taattgtata gttgataatg tttcttctgt
     1681 accaagagct gttttgactt gaacatctag ttttggtccg taaaaggctg cctcgccttc
     1741 tgcttcaaag taatctagtt ctaattcatc catagcggct ttcagcattg cttgggcttt
     1801 ttcccacatt gcgtcatcat caaaatattt atctgtattg tttggatcgc gatagctcaa
     1861 gcggaaacga taatctgtaa tattgaagtc agcatatact gccaccatca attcaagcgt
     1921 acgtttaaat tcatctttga tttgatcagg acgaacaaat gtatgaccat cattcaacgt
     1981 catttctcgt acacgttgta aacctgacaa agcacctgat ttttcatagc gatgcatcat
     2041 tcctaattca gcgatacgaa ttggtaattc acgataagaa tggatcgtgt ttttgtagac
     2101 catcatgtga tgagggcagt tcattgggcg aagaacaagc atttcgccat ctcccatatc
     2161 cattggcggg aacatgtctt cttgataatg atcccagtgt ccagatgttt tgtacaactc
     2221 aacatctccc attattggtg tatatacatg ttggtaacct agactaactt ctttgtctac
     2281 gatataacgt tcgatcgtgc gtcggattgt tgcccctttt ggaagccaga atggcaaacc
     2341 agaacctact tctggggaaa ccatgaacaa gtcaagttct tttcctagtt tacgatggtc
     2401 tctttctttt gcttcttcac ggagacggat atattctttc aaagctttct tgtcaaaaaa
     2461 ggctgtcccg tatacacgtt gcatcatttg attgtttgag tttccacgcc aataagcacc
     2521 tgccactgat aataatttaa atacttgtat acggccagtg gatggaacat gtggtccacg
     2581 acacaaatcg atgaattcgc cttgttgata agcagtaatc acttcttctt ctggcaattc
     2641 actaatcagt tctactttgt acggatcact tgcaaaaatt tctaatgctt cctgttttga
     2701 aagaacacgg cgttcgatcg gcagattttc tttgacgatt ttcatcattt ctgcttcgat
     2761 tgccggcaaa tcttctgcag ttacttggtt ttgtccatta tctgtatcgt agtaaaaccc
     2821 tgaatcgata gctggtccaa cgccaaagtg gatatttgga tagaggcgac gcatggcttg
     2881 ggccatcaaa tgagctgctg aatgacggac taagccaagt gcatcttcat ggtctggtgt
     2941 gatgatctca atacttgcat cttcctcgat tggacgagtc aaatcaacga gttttccgtt
     3001 cactttacca gctaaagctt tttttgctaa gctttttgaa atgctttcag caattgccaa
     3061 tgttgttgtt ccagactcaa attctttgac tgcaccatct ggaaaagtaa tttttatcat
     3121 aatagtttcc tccttgaaat gttttgattg atcagatcat caagctgaaa aacaaaaaaa
     3181 tcctagcatc tcttgaaaag agatactagg acgaaaagtt cgtggttcca cctaatgttc
     3241 aaacgcaaat gcgttcctca agcgttgtaa cggtccgacc gcccttgctt caacaagggt
     3301 ttcgaaagtg gtcatccttt ggttttctcc tggaaaagtt tcagcctagc ttttcctctc
     3361 ttatcgaaga ctctcaaatt cagttggctt tctcatcaat caattatgtg tttatttaac
     3421 cactaataaa tagaaaaatc aagtgggaaa ctaaacttat tttttccatt ccccatttac
     3481 accgtatgtt ccttttttca tctcattgat gatgacatgg atgtgttctt ttggtgcctg
     3541 cgtgttttta tgaacagctt ccgtgatatc tttcatcata ttttctaatt gttccggact
     3601 gcgaccttct actaattcga catgtacgaa tggcataatg ctctctcctc tttctgatcg
     3661 ttcttttttc aactcgattc tatcatacta tgtttgtctt gggtactgaa tcaggaaaaa
     3721 tggctggatc tttttttgct ctcaaggcac tcaaaataga gaccgtatcc acgacttctt
     3781 gcaaaacagc tccgaataat gcggggatta gtcccgtact tgcaattaac atcaaaaaaa
     3841 tacaaataaa gatgccaatc agtacagact gtttcgctat tctcatcgta tctcttgaaa
     3901 tcactactgc ttttgctaca cgcaagagat catctttcaa gatgaccgca tctgcacttt
     3961 cactagcagc tgtagaacca tgagccccca ttgcaatacc gatatctgca gcagcaagtg
     4021 ctggtgcgtc attcacgccg tctcctacca tcgcaacagg tctttgatct ttagggatac
     4081 tttctaaaat ggtcagttta tcttgaggca agctacgggc atgtacagaa tctaattgga
     4141 ctttttctgc aatcgtttga gcaatggcag atccatcccc ggtgatcatc atcaaatttt
     4201 tcacatcaag ttttcttagc tccttgattg tctttgctgc ttctgggcga atctgatccg
     4261 aaaataggat cgttcctgca aaagatcgat caattgttac atagattgct gtattggacg
     4321 tttctatttc ttcagctgaa acaaaagagg cacgtccaat ctttacttca tgattattga
     4381 ctactgcttg gattccttga ccggtcactt ctttcagttg actaacagga tacagttggc
     4441 tgtttttcac gttgttcact aacgaacgag ctaagatatg gctagattcc tgttctgcac
     4501 tagctgcata cagaagtagc tcttcttttt ttattccatt gactggctga atcgcctcta
     4561 cttccaatat ccctttggta agcgttcctg ttttatcaaa agcaatcgtt tttgtacgtg
     4621 cgagcttttc aatcgttgtt ccagttttta caacgatccc gtttttactt gaacggctca
     4681 ttcctgcaac taaagcaatg ggcgcagcta gaatcagtgg acaaggtgat gccactacca
     4741 gtacctcagc aaatcgtaaa gggtcttttg agaaccacca agaaatacca ccgatcaaat
     4801 aagcaatcaa cgtgaaaggg actgcataac gatcagctaa acggacaaat cgggcaggct
     4861 tttcttttga ttcttcgact agtttaataa tcatttgata ctggctgtct gcggcacgtt
     4921 tttcagcgat catttggatc gatccgtctc catttaaaga ccctgacata atctgatctc
     4981 caggtttctt ggtttccggg cgggactcac ctgtcaaaga agattcatcg accgaagatt
     5041 ctcctttggt cactcgacca tctgcgggta caagttcgcc aggcttgact agtaactggt
     5101 catcaatatt tatttcatcg attactatat cctgcaattt tccttctttg aatcgatggg
     5161 caattcttgg ggtgttatct aacaaagact gcagttcttt acccgctcgt ttattcgcgt
     5221 aatcctctag actatctccg cccgtaagca tgatcaatat catcaaacta gcccagtatt
     5281 ctttgacggc taatgttgca aggattgcgg taatcgctaa tacatctacg ccatatcttc
     5341 ctgaacgtaa cgtcttgatc atttccaaaa acatcaagaa ggcgagaatc cctccagtaa
     5401 atgcgacaat cgcaaatgca agggccggct ggtgaaaaac aaattcactt aataaagcta
     5461 tcccaccaat aagcaaagta agtaaaaatt ttttgaaatg tgtcattgtt ttttctcctc
     5521 ttttatgtga cttagagagg ttagtaataa atttccagct gcctctttca tcttaagcat
     5581 agtccttgac tagcactttt taaaacaata ggccttaatt gaaaacaact atcactatca
     5641 gcgtttccaa aattcatcag ttctacaaac aaaaatattc agcaaggatt actttttcga
     5701 ttcatcagtt aaaaagtacc ttttttcgaa aaactatgct atacttttaa ttcgttattt
     5761 acggcggaag gagcaaacat gcgatgaatg agaaacaact agaacaaacg cacgtggatg
     5821 aaacgatcga actaatccag atcgaacaaa atatgctgga tacaaaacaa caagaattga
     5881 ctcaggaaat gaatcattcg acaaaagaca cagctaatca caagattcga gctggttcca
     5941 atgaagcttt ttatgaatct gctgttgaat atcgccaaca tgagcaggaa ttgcttttga
     6001 aataccatac tcttgaagca cagcaaaaac gcctgcaaac gttgtctgtc atgaaagata
     6061 gtccttattt tgcccgtatc gactttaaag aagaccgtga aacagaaaca ttatatctag
     6121 gtatcgcttc tttaagagat ctggcagaag aaacaatcgt tattgactgg cgcgcaccaa
     6181 ttgccaatct ttattatgaa ggtgagattg gtcccgcatt ttatgaaact gaaacagata
     6241 gaatagatgt cgatctgcag ttgaaacgac aatttaaaat ccaagaagga cgtattttat
     6301 ctatggtaga tacgtcggaa gtaatcaatg atgacttttt gctagagata ttggatgaat
     6361 catcaagcaa ccatatgaaa aacatcgtct ctacgataca aaaagcacaa aatgcaatca
     6421 ttcgggacac aacaagtaaa gtaatgctga ttgaaggaat cgccggaagt ggaaagacat
     6481 ccgctttgct tcaacgtatt gcttttctgt tgtatcataa tcgcaagtgg ctcgaggctg
     6541 aaaacgttct tctattttca cccaaccatt tgttttctga ttacatctcg actgtattgc
     6601 cttcattagg tgaaagcggc gtgcctacac agacattcaa aaattatttg gtgcagctgc
     6661 tgccagagtt ttctttacta gatgaacagc aacaagaaag tggttttttg ctaggggaaa
     6721 aagatccgat acaagtgatg aaaagcggat tgaccttagt cgatcagatt gatcgctata
     6781 tccaaggcat cacaaactac ggtcccttgt tccgtgatat gaaaataaat ggccgtacga
     6841 tcctatcaaa ggaatcgatt cgccaatggt ataaagaaac aaatgaacaa ttacctttac
     6901 atcatagact ttctctatta caaacgaagc tcctgaaaaa attaggcggt ctgcaaaaag
     6961 acgaaacacg tcagcaatgg gtcaaagatc tggcggaaga acagctccaa gaactatacg
     7021 caacagatcc aaatcttgaa tatacagaac aaaaggagcg ttcattacgt aaaaaactgg
     7081 ccaaccaaat cgtacgtaaa agattccgtc gaatccatcg agggatcatg aattatcaat
     7141 ttgtcaatat aacgaaacaa tatctccact ttctacaaac tgtgccactt tctcttctaa
     7201 cacgacataa aatcagcgag accaggtggc aggaacattt gacaactgtt cgtacacact
     7261 tgcgtcaaag agaattgcgt caagaggatg ctgtactatt ctttttactg atgcgccgaa
     7321 catatccagt gacggttaca cacaaagctc gctatatctt tattgatgaa atgcaggatt
     7381 tcgcaccatc acaagtggca ttgctgcaag ccttgttccc aaaagcaaat ttgactttct
     7441 gtggagatct aaatcaaaat gtctttggaa atgaaacgat cgttggatca ttggaagacc
     7501 ttttcgtgga ccgtgaagtc actcgtttcc aattaacgac aagctatcgc tccacaaaag
     7561 aaatcactga ttttgctaat cattttattg tgaaagaaca tcaagtagaa acaacagcaa
     7621 gaaaaggaga ccttcctttg ctcatccaag gaacaacaga tgatatcaaa ttgaattggt
     7681 tagcagatac cattcgaacg actcagggaa aagcccggta ttggcgtaca gcaatcatag
     7741 ctaaaacaca agaagaatgc gaagctcttt atgctcaact gccagacgag ttgaaacaac
     7801 aagtccagct gattttaaat gagagtgact ttatgaagcg ttccattatt ttacttccag
     7861 cctttttagc aaaaggtctg gagtttgacc gagttttctt gtgggatgta gatgcagaac
     7921 atttttcttc tcagcaagac aaattgatcc tttacaccat gtgtacacgt gcgatgcatg
     7981 aattgaccct ttttacctcc aataatgaaa gtcctttgtt aaacaatatg gatatttcat
     8041 cttatgagaa aaaagtactg taagtaaaaa gacaactgct atcattaatg ttagcagttg
     8101 tctttttatt tacaataata attgcagaaa aagtaattca atcatttaag gtcatttatt
     8161 ctatatataa atccaaatct gtttcaggat attttaattt gacaagcgaa ataatcattt
     8221 tcagttcttc catgttttct ggatattctg ggtcaaatac cttgctatga cggccaatcc
     8281 cgccattgtc gatcaatcgg taaattcgat tccattcaat gaattggtca attgattgga
     8341 aattgctgat tggatagccg ccacaatcca aaatctctga tacacgaaat gctaggtatg
     8401 cactatcgat cactttattt tttttcgtgt ctatcttctc atttctttcg ataagaattt
     8461 tttcaggacg atgtttcaag ctccactcta atagtagaaa gaaagcctcc atccgatact
     8521 cttcttcaaa taaccgaaag gctgaatcag aacgacgaaa aagcagcgga atcagataat
     8581 tttgtctata aatcagcatt ggtgcgcggt ccaccataca ctctaaataa cggttcagat
     8641 accctgtacc gacatagttt cctaatcgat agccttcaac gccgaatctt gcgtattttt
     8701 gcgctaattt tgaacaatca gttacgttca cagctaactc ttctactttg cggtaaccgc
     8761 ttaagatacg ttcaaaaggt tctaaagtga tttttgcttc catattcttc cccgtccatc
     8821 ttccacatac ttcatatcat ttcacatgat acttatgctc tcatcatacc acaggttgaa
     8881 ttagaaaaga aaataaatca cacaaaaaag caagacaatc tgttagtgag attgtcttgc
     8941 ttttcgaaaa agactgaata cttttcgtta aaactggttg cttagtcttc taatgccaca
     9001 ttatggaaca catgctgtac atcttctaga tcttccagtg catcaatcat tttttcaaat
     9061 gtttcttgat cttctggaga tagtgttacc tcgttttgag ggatcatttg gatctcagcg
     9121 attgaaaaat cagtgattcc tttttctttt aatgcttctt gtatactatg gaaatccgct
     9181 acttcaccgt aaacgatgat ttgtccttct tcttccatta catcgcgaac atcgatgtct
     9241 ttttccatta ggtattcaag gatctcgtca gcgtcttctc ctgcaaaagc aaagattgct
     9301 gtattgtcaa acatgtaaga aaccgctccg gaaacaccca tgttcccgcc atttttccca
     9361 aatgctgccc gtacgtctgc tgctgttcga ttcacattgt tcgtcaatgt atcaacaatc
     9421 accattgagc cgcttggtcc aaatccttca tagcgcaatt cagagtatgt ttcatctcct
     9481 gcaccttttg ctttttcgat tgctcggtca ataatatgtt ttggtacatt ataagtttta
     9541 gcgcgatcaa tgacgaaacg tagtttttgg tttgcatgcg gatcaggatc acctgatttt
     9601 gctgccgcgt agatttcaat cccaaatttt gcataaatcc ggctattatt agcatcttta
     9661 gcagttttct ttgctacgat atttgcccat ttacgtccca tattttcact tcgctttctg
     9721 atttgatagt ttcataacgc aaaaacgtcc tgttccatta tacttgtgaa tcacgattct
     9781 gtgaagcttt ttttctttct tccttcattc ttctcatttt tagtcaatta tctatactcg
     9841 gattgggatt tgcccgaaat tctgttccgg aatattgcct cccaattgat agatttgatc
     9901 acttctaact aacagttccg cttcttgtgc ctcttttttt ccgattcggg aataaataga
     9961 aatctctttt ttttctttta agaatcttct tccagcatcg gtaaatccaa gtagtcgaag
    10021 ataattttta ttttgttgga caggaatctc ttgctttttg atattcagca agacataaca
    10081 agataatcgt tggatccgtg tccatgtata tctttttgtt tttacctttg acacaaaatc
    10141 ctcaaaacta tcagcagttg cagctgctgt tttcatgcgc gattctattc cttcggtcat
    10201 ctgatagata tctgtcagtt ccagcaaagt agaagataac aaccgataac gcaaatacgg
    10261 ccaataagat tcccaagtct gcagcgaaca agttgaaaga tcttcttctg tctgaggcgg
    10321 cacaagtcca gctattcttt ttccttcttc taacgcatgg cgaatggctg ttgcactggc
    10381 tatcctctga tctgtgatga ttttttcatg atagcctgca cttttacgtc ggatcggatg
    10441 taattccata gggtggacat acttggcatt ttcctttgca taacttaatc ccaagatatg
    10501 atttggcgaa gaaaaatcaa gcgtaatatc gggatacaga ctgcgaaaaa cctcagtcat
    10561 tttttgcgga taactatacg tttgatcaga cagttcttga aatgcctctt ctatcctatc
    10621 ttgattctct tggacaaagc gtccgaaact gctgtaatca aacgaagtct cttcttctgt
    10681 tccaaaacac aaactgtcac agtccagtgt ttgcaagatt tttactgcac ctttagcaaa
    10741 atagtcagct gactgaaccg cccattcgac gggaagctcc actactaaat ccacaccatt
    10801 ttgtaaagct tcattcgcgc gatgccattt atcgatgata gccggttcgc ctctttgcaa
    10861 aaaattcccg ctcattacag caatgacgac ctctgctcct gttatttttc gtgccatttc
    10921 cacatgatag cgatgtccat tgtgaaaagg attgtactct acgataatcc cgcagctttt
    10981 catgcgcatc ctccttcata gttatcgata aaaaagaaat agagacaagt aaaaaaaggc
    11041 aaaaatcgga aaaaataccc gaactatata aactattctc gcaaacacaa tacttctgcg
    11101 acgaaaagct gcactgcaag agcaatgcct atatcgttcg gatatataat ttatgttgaa
    11161 gatttattct tttcttgtta actcgagcag gcaaaaccgc tagtctttgt atgctacaaa
    11221 gaaccagcgc tttgatttct ctgatgctgc ttcgtctgta aaatcagaaa atgcttcgac
    11281 tttcgaaaag ccgctgtttt ccagcattct taaataactt tccaatggat aggtacgctc
    11341 ttgatgtagt tcatcttcac gaataaatcg accagaccct tcttgttctt caacgaaaaa
    11401 ggtaagaaaa tgttcgatgc tgtgtttctc gcttcctgga taactatccc acaaaaaagc
    11461 aaaattatcc ccttgataat gatagctata ttctggaaaa acctcatcta cttgataggt
    11521 ggagtgtacg tcaaataaaa atcggccatt ttcttctaac gcttgataga cttcatcaaa
    11581 cacttgctgt acctcttgtc tatttttcat atagcacagc gaatcagaaa aacaggtaat
    11641 tgcctgatat tgtccgattt ctgataaatc gagcatattg ccttccacaa attggatcga
    11701 tgcgtcttct tcatatgctc gctggctggc aatcatcaac atctcttctg aaaggtctaa
    11761 ccctgtcact ttatatccat cttttgcaaa ggcaaccgct aatgctccag taccgcatgc
    11821 cagttccaaa atattttttg tgtcttcttg taaatgacgt ttcgaaaaag ctaaccattg
    11881 ctgataaagt gtttcgtcca tcacttcatc gtaaatgaaa gcaaatgttt cataagccat
    11941 atgttaatcc aaccattcac taagatcgac tagcggagca tctgaccata gtttttccag
    12001 attatagaaa tttcgttctg gcgcatggaa aacatgtacg atcacatctc caagatcgat
    12061 caatacccat tttccgcctt cttttccttc aatacgtttt acttcatatt tgttttcttc
    12121 ttctttatca atgatttctt ctgtaattgc gttgatttga cgttcgctat ttgctgaaca
    12181 gatcatgaaa taatctgcca acaaggaaac atttcgaacg tctaaagcga taatgtcttc
    12241 tgcccgtttt gaatctgctg cttcaatcgc aatttttaat tgttctttac tatctatgat
    12301 gatttcctcc taatatgagc agcttatttt tttgctaccc agtaattata tgtttcgatt
    12361 gtttttggat aaatctgttg ttcttgcgca agcaaatggg ccaatgtatg ttttgtttca
    12421 aatgctacag cttcgtctag atcagcccat gcaatggctc gtgcatcttg taccccagga
    12481 aaatctcgac ctggttcaat atagtctgct acataaatga ttttgtcaag gagactcatc
    12541 ttagcagccc cagtcgtatg cacccggatt gcatgaagaa tctctgcatc ggtgattcct
    12601 aattcccgct ctacaaacga agcgccaaca agaccatgcc agattgcatt attataattc
    12661 aataactccg gatcaaaacc gtctcttacg attaccattt tgaactcctc atctggtcgt
    12721 tcttttgcat aatcatgtgt caatgcagca atactagctt tttcaggaga agcaccgtat
    12781 tttttggcaa gagccacggc tgtttcttct actccaagca catgcttgaa gcgttgttcg
    12841 ctcattctca tctgtatttt ttgcatcagg ttttctctag aataagcagt atattcatta
    12901 ctatagtcca tattcataca acccttcatt ttgaatataa tcaattactt tatctggaac
    12961 aaggtagcgg atagaacagc cttctttgat cttttgacgt attttcgtgg aactgatgtc
    13021 gatctcaggt acatctaccc aaattacagg atatggagta tccgttgtgt atcctggtcg
    13081 acgtataccc acaaagttga ccatagaagt cagctcatcg attttatacc attttggcaa
    13141 atattctacc atatccccac cgataataaa gtaataatct gtatccggat tgttttgcgt
    13201 caattccttc atagtatcat aggtgtaact tttcccacca cgagctaatt caatcgtttc
    13261 aatctgcaaa aaaggattgt cttccaccgc taattccaac atgttgagtc gatgttttgc
    13321 atcaatcgtc tgtttctcat caacatgagg tggctgataa gaaggcatca aaaatacgcg
    13381 gtcaagacct aaattccggc cagcttgttc agccatcact aaatgtgcaa ggtgaacggg
    13441 attgaatgtt ccgcccaaga tccctacttg ctttttcttc tctaagaatt gaggcatttc
    13501 ttcaggaaat aattgtgaac gaacaaatgt cttagcttgc gtattcatca tggtcactcc
    13561 ttatattgct tttacttctt ttgatagcct ttgatacttt tcttttgtgg aaggtttgta
    13621 taatacaata acacgtccaa tgatttggac gatttcgcaa tctatttctt tttctaatac
    13681 ttgagctgct tcttctgcaa tctcatctgt attttgaagt aacgatactt tgatcagttc
    13741 tcttttttct aaagcttctt caatttgaac gatcattgct gaattgatcc caccttttcc
    13801 gatttgaaaa atcggctgaa gatgatgcgc tttgctacgt aaaaaacgct tttgttttcc
    13861 tcttaaactc ataattttcc ttcttcctta aatcaatgct ttgcgtttca atacatctac
    13921 accctttggc gcccagcccg caattactac tggcgctgtt acagtgatcc agcctagacc
    13981 ggcaaaaacg atgtctgttt tttctttcac agaaaactca aaacgaacaa gctccggaaa
    14041 tgcctcgact tcatcaggac gaggaggctg taataatcca cctacatgtt tttgataaaa
    14101 ttcgtctgct ttttccagtt tcgttcgatg aatatttaca tcatttgaca cataagcaat
    14161 aaatgaagca cgattgccgc tgataaaatc aaatcttgca agtccgccca agaaaagtgt
    14221 ttgcccttca ttcagctgat aaactttagg tttgatttct tttaacggtg caatgatccg
    14281 caaatctttt ttacctaagt agtgcgccat ttgatggcga tggataattc ccggtgtatc
    14341 aatcaaaaaa tgcccgtcat ctaaaggaat ctcgatttta tccaaggtcg ttcctggaaa
    14401 ctgcgaagtt gtgattacat cttgtactcc cgctgtattt ttgatgattt gattgataag
    14461 cgtcgatttt ccgacatttg ttactccgac gatatagaca tcccgtccat cgcggtattc
    14521 ttcgattttt tctaatagtt ccgtcatctc atgagatttc ttagcactag ttaataatac
    14581 atctactgga cgaattcctg cttcgtaagc acgttccttc atccattgtg ttaatttagt
    14641 ttttttcaat gatttaggta atatatccac tttgttccca actaaaagta ctggattgtc
    14701 accgacaaaa cggtgtagtc ctgggattag tgaaccattg aagtcaaaaa tatccaccac
    14761 attgacgatc aatgcatctt tttgccctaa ttcattcagt aagcgcaaaa agtcgtcatc
    14821 cgtcaatgag acatcttggg tctcattata atggcgtaat ctgaaacaac gctgacaata
    14881 gacttccccg gaagctaaac ctttttccaa agccgtttgc ggcgtgtatc ctaattcacc
    14941 gggtttgttt gtttgtataa cggcaccaca gccgatacat tgtaattctt ctgtcattcg
    15001 attccacctc gccagcccat atcagggtgt tttgctaata gataggccat aattcttttt
    15061 tcccaaaaac gattaaattg tgtcttccaa ctatctgttg cgataatggg ttttacaagg
    15121 atgctacgta tccctgcacg attggctcca cgtatatcag tcataatctg atcgcctacc
    15181 attactatct catcatctgc taaatccaat tgtttctttg ctatgttgat ccctcttgct
    15241 aaaggtttca ttgctcgtgc aacatacaaa agatcaaatt tttcaatcgc tcgcgcgaca
    15301 cgttttgaat tattgtttga aacaacaaca acagggattc ctgcattttt catttctaaa
    15361 atccaagtaa gcaactcttc tgttccatct ggattattcc atgcaatcaa cgtattgtct
    15421 aaatcggtca atactgcttt gatgcctaga ttctttaatt gggcaggtgt aatctgatag
    15481 atggcttcta ccatccatgt tggtttgtaa tttgaaaaca tgctgtctcc tcatctgcaa
    15541 aaaatgaagg agcacataaa gtactcctca accccgtatt atactgcata tcagcaaaaa
    15601 aaactacctc tatactcgtt cgtctttcag aaaaagcatt tctttacaaa atgaccaaaa
    15661 ataaagattg atcacagaaa aatttagaaa caaaaaacaa ctaatattgg cggaacctct
    15721 tgtatcgttt ttccagtaat ttatgctgat ctaatcgaac aagttccgtc aatctttcaa
    15781 tcaacgcttt tttcataatg tgtgcgactt tatatcgtgg caactgctcg ccgttgaacg
    15841 tttcaggaat aactcgatca acgattccta actcgcgtag ttctggcgca gtaattttca
    15901 tcaactcagc tgcttctgcg gcgcgactac cgtctttcca caaaatcgac gcaaatcctt
    15961 ctggagaaag cacagcataa atcgtttctt ccagcatcca gacttcatcc gctaaaccta
    16021 gagcaagggc accgccactt ccaccttcac caatgatgat ggcgatgatc ggtaccttta
    16081 aatcagacat ttcgagcaga tttctggcaa tagcctctcc ttcgccgcgc tcttctgctc
    16141 ctacaccgca atatgcacct gctgtattga tcaaggtaat caccggtcta ttgaactttt
    16201 ccgcttgttt catcagtcgt aaagcttttc gatagccttc aggatgggga gaaccaaaat
    16261 ttctgcggat attttcctgc agatcccgtc ctttttgaat accgatcact gttaccggtt
    16321 tgccatctaa tcgagcgatt ccaccaacaa tcgcttcgtc atctgcaaat aaacgatcac
    16381 catggcattc ctcgaaagaa tcgaagatct cttcaaaaaa atccaagctt gtccatcgtt
    16441 ctttagctct tgctaactga acgacatcat gtgctgtctt atccacgatc tatccatcct
    16501 ttctgtgtat gcaattcaag aagcgtatgg attttctgtt tcaattccat gcggggaaca
    16561 atctgatcga caaaaccatg agataataag aattcagcct tttggaaatc ttctggaagt
    16621 tcctgcttga tcgtctgttc gatgacgcgt cttccagcaa aaccgatcaa gctttggggt
    16681 tctgctagaa taatatcgcc ttccattgca aaactggcag ttactcctcc ggtcgtcggg
    16741 tccgttaaaa cagttagata aaaaagacct tcattgctat gccgtttgac agcagcagag
    16801 atttttgcca tctgcattag agaaaagatt ccttcttgca tccgtgcgcc acctgaagct
    16861 gtgaacaaga cgactggaag actttccttt gttgctcgtt cgaataatct ggtgatcttt
    16921 tctccgacaa tcgttcccat acttcccatg ataaaattag gatccatcac tccaataatg
    16981 aagggaatcc cctgaatggt tgctttacca gtgaggactg cttcatgaag tcctgtttta
    17041 tcttgcattt ttttaatttt ttccgtatat tcgggaaatg ctagcggatc tttggttaca
    17101 agatccgtat cccattcttc aaaacttttt tcatcaataa tcaaagctag acgctgccat
    17161 gcaccgattc gaaaagcgta tccgcaatga gggcatactt tttcttcacc gatgtctttc
    17221 gtataaagga tatgtttgca atttggacac tttgcccaca tgttatcagg aacagatggc
    17281 gagttagcag gtttgctctc tctattagga ttgatccgaa tatatttctt tttcttaaac
    17341 agagccatct atgcgcctcc tatctccgtt tctggtgtcc aattcggtaa aaattcttct
    17401 tgtaaaaatg ctgtgctgta atcacctgct atgactgcag gatgactaat caaatccatc
    17461 tgaaattctg cgttcgtgat gattccttct gtgataaatt cacttaaggc tcgctgcatc
    17521 ttcattaatg cttcaaaacg cgtatttcca tggacgatta tttttgcaat cattgaatca
    17581 taatatggcg ggatcgtata tcccgggtaa actgcactgt ctactcgcag tcccatccca
    17641 cctgctggaa gcagtaagtt ttggatcttc cctggagaag gagcaaaatg gaaagcagga
    17701 ttttctgcat taatccggca ttcgattgcg tgtccttgga atactacttc ttcttgacac
    17761 acattcaaag gctcaccggc tgctatctcc acctgttttt ttactaaatc gattcctgtg
    17821 accatttctg ttactggatg ttccacttgg attcgagtat tcatttccat gaaataaaaa
    17881 tcgccttctt catccatcag aaactcgatc gttccggcat tttcataatt gactgcttta
    17941 gctgctcgaa cagcagcgtc tcctagtgct tctctttttg tttgtgagat cactacagaa
    18001 ggagattctt ccagtacttt ttgattgttg cgttgtaaag aacaatctcg ctcgcctaaa
    18061 tgaatgacat gaccaaatcg atcacctaag atctgaactt ctatatggcg agcaggataa
    18121 atgatttttt ccaaatacat atcatcattt ccaaatgccg ctgctgcttc ttgctgagct
    18181 gaagaaaaat gctgtgggag ctcttctttt gattgaactt tccgaatgcc tttcccgcca
    18241 ccacctgcag ctgctttcag catcactgga taaccaattc tgtctgctat ttccaatgct
    18301 tcttctactg tagataatac accagtactt cctggaatga ctgggacatt tgcttcttgc
    18361 atcaattgac gcgcattgat cttatttccc attgcatcga tcgttgcact tttaggacca
    18421 ataaatgtga tattgcactc ttcacacatg gaggcaaatc ggctattttc agataaaaaa
    18481 ccaaatcctg gatgaattgc ttcggctttt gtcacgattg cagcgctcag tacttcctga
    18541 acattcagat atgaatcaac ggcttttgcc gggccaatgc agattgcttc atctgctaac
    18601 tgtgtatgaa gtgcttcggc atctgcttct gaatatatcg ctacggtttg gatccctagt
    18661 tcgcggcatg cacgaatgat acgtacagcg atttcaccgc ggttagctat caatacttta
    18721 gaaaacatat ttcacctatc caatcataaa ggttaattcc gcttcggcta cttttttacc
    18781 gtctacttta gcgattcctt tgcctattcc ggctgaagct ttcactttca atatctcaac
    18841 ttcaaggtat aaggtatctc ccggtgtaac tttcttacgg aattttgctt tgtccaatcc
    18901 gccgaaataa gccgtctttc ccttgaattg ttcaagagaa agtaatgcaa ctgctcctgc
    18961 ttgagccatt gcttcaacaa tcaatacccc tggcataaca ggttcttctg gaaaatgtcc
    19021 ttgaaaaaag ggctcattga tcgttacatt tttcttagca acgatccgtt ctccttcaac
    19081 catttcttcc actcgatcaa tcaaaagcat tggataacga tgtggaatga tttcttttat
    19141 ttcttgtatg ttcattgttt tccctctttc ctgctttgct tatttttgct gaatcctaaa
    19201 gagtggctgt ccatattcga caactgactc gttttctaca aggatttctg tgatcactcc
    19261 gtcggcctca ctagttatct cattcatcag tttcatcgct tcaataatgc agataacttc
    19321 tcctttggca acttgatcac ctactttttt gaaagctggt ttgtcgggcg ctggttgcag
    19381 ataaacgatt cccacgattg gcgaaacaac ctcgattcca tcaaatactg ccgattcttt
    19441 ttctttcatt atttctggag cagttgttgc ttgttcaggt atgacattga ctggctccga
    19501 aactggcaac gtttcgtttg tttgaaccat attcatgcct cgacttgttt tatttttatt
    19561 catatacaat tcaaattgac cttcacgcaa atcgaattcc gttagcgagg attgatcaaa
    19621 ttgactgact aattctctga tttcattgat atccaaaatt attcctccca gcgtttcatg
    19681 cacagaaccg cattatgtcc gccaaaccct aatgaattac tcattgcata agtaaaactt
    19741 gcaggtttgc tttcgttttt gacaactgtc aagtcaatct caggatcttg tttttctaca
    19801 ttgatcgtag gtgggataaa ttgatgttct aatgccagca aagttgccaa ggcttcgatc
    19861 ccgccggctg ctcccaataa atgtccagtc atcgatttcg tgctgctgac gtaagtatct
    19921 ctgaaatttt cacctaatgc gatttggatt gcttttgctt ctgcctggtc attggctggt
    19981 gtacttgttc cgtgagcatt gatatatccg acttgttccg gctgtacacc agcttcttcc
    20041 atcgcaagct tcattgcttt agatgcacca gaaccatctg gagtaggagc tgtcatatgg
    20101 taagcgtcac agtttgcacc gtatccaacg atttctccat agattttggc accgcgagct
    20161 tgtgcatgtt ctaaagattc aagaactaga acgcctgctc cttcacccat cacaaaacca
    20221 ttccgatttt cgtcaaacgg aatcgaacca cgattaggat cagttgattc tgagagtgct
    20281 gttaaagctg caaatccagc aatccccatc tcacaaatcg ttgcttctgt tcctccagca
    20341 agaatgacat cagaatagcc atgtttgatg ttacggaatg cttccccaat tgaattcgtt
    20401 ccgctagcac aagctgttac gatagctgta caaatacctt ttgcacctac acgcaaagca
    20461 atatttccag ctgccatgtt ggcaatcgcc attggtacga acattggaga aactcgtttt
    20521 gcaccttttt catgtagacg aataacttgg ttttcgattg ttggaagacc accgatccca
    20581 gatccgatga tgacgccaaa ccggtcttga ttcatttttt ctttatctaa accgctcatc
    20641 tccattgctt ctaaagcagc gtagattcca tagatcgaaa acatatccat tcgtttgcta
    20701 tcttttttaa taaaatattt atcgtaaggg aaatctttga cttcacccgc taaggtaatc
    20761 cctgtttctg aagcatcaaa tttagtgatc ggtgcgatgc cattcgtccc ctcttttaag
    20821 ctattcaaaa agctgtctgc gtcatttcca ataggtgaag tgactccata gccagtaatt
    20881 accactcgat tcatttgttt gctctccttt tcttatccgt gcatgaccaa tccgccatcg
    20941 acattgatga cttgtccggt gatatatgga ttttgtgcta agaaaactgc tgtttgtgca
    21001 atatcttcta cttgtccgaa acgctgcatc ggaatttgct tttccgcttg ttccttgact
    21061 ttgtctgcta aaacttccgt catatcagtt gtgatgaatc ctggcgcaat ggcattacat
    21121 gtaatgcctc tagttgcagc ttctcgtgcc acagattttg ttaatcctac tacgccagct
    21181 ttactagctg cataatttgc ttgaccaata tttccaatca acccagatac actagagaga
    21241 ttaatgatgg ccccttctcg ctgcttcatc atttttttca atacgtgctg cgtcatattg
    21301 aaggttccaa tcaaattgat atccagacat tttttgaaat cttctgcatc cattcgcatc
    21361 acaagtttgt catttgtgat tccggcattg tttactaaca catggatcga tccaagcttt
    21421 tcttctgctt ctttgatcat ttgtcccgct ttctcataat cagagatgtc accggaaact
    21481 cctacgcatt ttacaccgaa tgcttcgatt tcttctatct tttcttttgg aatctctccg
    21541 cgaccattca aaatgatatt tgctccagct tttgcaaaag ctaaggccat cgctttgccg
    21601 attcctcttg tactacctgt gataaataca ttttttcctt ttacatccat aacgttcctc
    21661 cttatagtat tgctattgtt tcatcaaatg tctgcttatt ctcaacacgc agaactggta
    21721 ttgttttatc gatttttttc ataaatccgc ttaatacttt tccggggccg acttcgatga
    21781 cttgttcgat gccgattgtt tttaacttat ggatactctc atagaaacga acaggttgca
    21841 tgacttgctg ttcaagtagt cccgcgattg tttctttctt cattatttcc gttgttgtgt
    21901 tgcttatgat aggaaaagaa ggttcagaga attgaatctg acttaggtcc ttcgccagtt
    21961 tttttgctgc cggttctaaa atagctgtat gaaaaggccc gctgacattt agagggatca
    22021 ttcttttgaa ccctttttct tttaatagta aaacagcttc gtctactgct ttttcttcgc
    22081 caccaatcac gatctgctga ggtgtattat aatttgcagg agatacgatg ccgtacttgc
    22141 tagcttcatg acagctttcc tcgatcgttt caatcggcgc attcatcact gcaaccattt
    22201 tcccgcttcc agctggggca gcttctgtca tataagcgcc tctttttgct actaacgcaa
    22261 ctgcttcagt gaaggatagc gcattactag ctactaatgc cgaatattct cctaaactca
    22321 gtcctaaagc tgcatctggg atgattccat gctccttcaa taagcgataa tacgcgatgc
    22381 tgactgttag aatagctggc tgtgtatatt gcgtctgatc caaacgttca ttttctgtaa
    22441 agcaaagttc agccatatcg tagccgagaa tctcacttgc ttcatcaaat gtctgcttta
    22501 caacggcttc ttcatataaa tcttttccca taccttgata ttgtgcacct tggccactaa
    22561 ataagaatgc tgttttcatg tttttctcca atatatccag aatatatgtt tgattcttcc
    22621 tattcaaaaa ggccgagaca ttcctatttc aggaaatgtc ccagccccca acctaatttt
    22681 aatgattatg cagtttgttt ttctacgtaa gtcactaaat cttgaaccgt tttgattccg
    22741 tcttctgttt cgattttcac gtcaaattca tcttcaattt cattgatgat ttggaataaa
    22801 tccaagctgt cagcttctaa gtcttcttgg atgtcagtag tcaatttgat ttcttcagtt
    22861 tcttttccta attcttctgc gatgatttct tgaatttttt cgaataccat aataatatat
    22921 tcctccgata ttgtttgctt tttattttta taatttaaca gtctacaact ccatgagaat
    22981 acttccccat gttaaacctc cgccatagcc tgtaaatatt actttctgct gttttcctaa
    23041 ggtgatcttt ccactagaaa ctgcttcatc caataagata gggatgcttg cagcagatgt
    23101 attcccatat ttatccatat tggtcaggaa cttttcttgc ggcatcttta cttttcgggc
    23161 aattttctca ataattctga ggtttgcctg atgcaaaagc agataatcaa ctggtttgtc
    23221 tttcatgact tcttggatat tgtttgcaac gtctcgtacg gcaaaatcaa aaatatctct
    23281 gccagtcatt tgcaaataat acgaaccttc tgaatcttct gaatagaacg gatttttatt
    23341 ttgctggtag ccagcggtca atgagctact tctggtaccg tcagctgcta atttttcttt
    23401 cagcaatttt ttctgtggat tcgcttctaa gagaatccca cctgctccat cgccaaacaa
    23461 cacagcagtc gtacgatcag accaatcgat tagcttagat agtgtttcac caccaatcac
    23521 cagtccctta gttgagccgc agcggatcat tttttcagcc atgcttaacg cataaacaaa
    23581 tcctgagcac gcagcactga tatcaaaagc gatagcatga gttgctccca atttcccttg
    23641 tacttgtgct gctacagatg gtgtcgtgta atcgggcgac attgttgcta ctaaaataaa
    23701 atccaaatct ttagggttta gatttttctg ttttatcaat ttttccgcca ctaaaatgca
    23761 taaatcagaa gtctcctgat ttgttacaca tcttcgttca cgaatacccg tccttgacga
    23821 aatccattca tcagaggtat ccatcatctt ggaaagatca ttattagtaa taatcttttc
    23881 aggaaggacg ctggctgttg cagtaatcat tccaaattgt tccattaaat atcctcactt
    23941 atagtcttgc aagaagtcgt gcaagttttt cagtgcatcc agcaaggcat gctgttcctc
    24001 cgtagccatt ccgtctaaga tgttcttgac catttcacga tgaaaatgct gatggacccg
    24061 aaatagtaac tttccttttt tcgttagtcc caactttaca acgcggcggt catcttcact
    24121 gcggatacgt tcgacatagc ctttttttac taacttgtta atagcagtgg tcaaggtccc
    24181 aacagtaatt gatagttctt tggctacttc tgaagttgtt tttttcttgt acatcccgat
    24241 tgcctcgatc gtgtgcatct ctgtgattga cagatctttg aattgagatt ttttcagctc
    24301 agactcttcg attgtcaaaa tatcattgaa gacactaaca agataatcat ttactgtttt
    24361 taaatctggt tccatgttcc accgtccact aattagtttg attatcaaat cttttgatgt
    24421 tcaaactata tcttaacttt tttc
//
LOCUS       DAZUKR010000030        23884 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000030 DAZUKR010000000
VERSION     DAZUKR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.030"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG89C_001085"
     CDS             complement(54..788)
                     /locus_tag="ACG89C_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4899634.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG89C_001086"
     CDS             1115..1525
                     /locus_tag="ACG89C_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4899635.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG89C_001087"
     CDS             1518..2219
                     /locus_tag="ACG89C_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS4899636.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG89C_001088"
     CDS             2219..3805
                     /locus_tag="ACG89C_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899637.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG89C_001089"
     CDS             3824..4045
                     /locus_tag="ACG89C_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS4899638.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG89C_001090"
     CDS             4042..4803
                     /locus_tag="ACG89C_001090"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS4899639.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG89C_001091"
     CDS             5183..5692
                     /locus_tag="ACG89C_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4899640.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG89C_001092"
     CDS             complement(5767..6306)
                     /locus_tag="ACG89C_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS4899641.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG89C_001093"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG89C_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4899642.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG89C_001094"
     CDS             7319..8170
                     /locus_tag="ACG89C_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4899643.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG89C_001095"
     CDS             8198..8833
                     /locus_tag="ACG89C_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS4899644.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG89C_001096"
     CDS             8853..9626
                     /locus_tag="ACG89C_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4899645.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG89C_001097"
     CDS             10034..10831
                     /locus_tag="ACG89C_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS4899646.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG89C_001098"
     CDS             10809..11222
                     /locus_tag="ACG89C_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4899647.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG89C_001099"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG89C_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4899648.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG89C_001100"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG89C_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4899649.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG89C_001101"
     CDS             15999..16559
                     /locus_tag="ACG89C_001101"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS4899650.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG89C_001102"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG89C_001102"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS4899651.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG89C_001103"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG89C_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS4899652.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG89C_001104"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG89C_001104"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4899653.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG89C_001105"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG89C_001105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4899654.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG89C_001106"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG89C_001106"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4899655.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG89C_001107"
     CDS             21906..22484
                     /locus_tag="ACG89C_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4899656.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG89C_001108"
     CDS             22536..23018
                     /locus_tag="ACG89C_001108"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS4899657.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG89C_001109"
     CDS             23135..23509
                     /locus_tag="ACG89C_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899658.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7809 a         4289 c         5123 g         6663 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtc
//
LOCUS       DAZUKR010000031        23064 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000031 DAZUKR010000000
VERSION     DAZUKR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.031"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..565)
                     /locus_tag="ACG89C_001110"
     CDS             complement(110..565)
                     /locus_tag="ACG89C_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899659.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="ACG89C_001111"
     CDS             complement(719..1927)
                     /locus_tag="ACG89C_001111"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS4899660.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="ACG89C_001112"
     CDS             complement(2039..2371)
                     /locus_tag="ACG89C_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4899661.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="ACG89C_001113"
     CDS             2519..3394
                     /locus_tag="ACG89C_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4899662.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="ACG89C_001114"
     CDS             complement(3476..4057)
                     /locus_tag="ACG89C_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS4899663.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="ACG89C_001115"
     CDS             complement(4063..5322)
                     /locus_tag="ACG89C_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4899664.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="ACG89C_001116"
     CDS             complement(5324..6370)
                     /locus_tag="ACG89C_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899665.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="ACG89C_001117"
     CDS             complement(6605..6880)
                     /locus_tag="ACG89C_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS4899666.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACG89C_001118"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACG89C_001118"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4899667.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACG89C_001119"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACG89C_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4899668.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACG89C_001120"
     CDS             complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACG89C_001120"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4899669.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="ACG89C_001121"
     CDS             complement(10708..11343)
                     /locus_tag="ACG89C_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS4899670.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="ACG89C_001122"
     CDS             complement(11409..12812)
                     /locus_tag="ACG89C_001122"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4899671.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="ACG89C_001123"
     CDS             complement(12799..13830)
                     /locus_tag="ACG89C_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899672.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="ACG89C_001124"
     CDS             13861..14082
                     /locus_tag="ACG89C_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS4899673.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="ACG89C_001125"
     CDS             complement(14247..14984)
                     /locus_tag="ACG89C_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS4899674.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="ACG89C_001126"
     CDS             complement(15137..16477)
                     /locus_tag="ACG89C_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4899675.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="ACG89C_001127"
     CDS             complement(16714..18237)
                     /locus_tag="ACG89C_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4899676.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="ACG89C_001128"
     CDS             complement(18615..19226)
                     /locus_tag="ACG89C_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4899677.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="ACG89C_001129"
     CDS             complement(19556..20272)
                     /locus_tag="ACG89C_001129"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4899678.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="ACG89C_001130"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="ACG89C_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS4899679.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21601)
                     /locus_tag="ACG89C_001131"
     CDS             complement(20831..21601)
                     /locus_tag="ACG89C_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS4899680.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="ACG89C_001132"
     CDS             complement(21598..21969)
                     /locus_tag="ACG89C_001132"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4899681.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="ACG89C_001133"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="ACG89C_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4899682.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaa
//
LOCUS       DAZUKR010000032        22853 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000032 DAZUKR010000000
VERSION     DAZUKR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.032"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..1485)
                     /locus_tag="ACG89C_001134"
     CDS             complement(154..1485)
                     /locus_tag="ACG89C_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4899683.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1634..2347)
                     /locus_tag="ACG89C_001135"
     CDS             complement(1634..2347)
                     /locus_tag="ACG89C_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4899684.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2522..3946
                     /locus_tag="ACG89C_001136"
     CDS             2522..3946
                     /locus_tag="ACG89C_001136"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4899685.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4245..4952
                     /locus_tag="ACG89C_001137"
     CDS             4245..4952
                     /locus_tag="ACG89C_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4899686.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4939..5262
                     /locus_tag="ACG89C_001138"
     CDS             4939..5262
                     /locus_tag="ACG89C_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4899687.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5351..6388)
                     /locus_tag="ACG89C_001139"
     CDS             complement(5351..6388)
                     /locus_tag="ACG89C_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4899688.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6709..7692)
                     /locus_tag="ACG89C_001140"
     CDS             complement(6709..7692)
                     /locus_tag="ACG89C_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS4899689.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(8107..8877)
                     /locus_tag="ACG89C_001141"
     CDS             complement(8107..8877)
                     /locus_tag="ACG89C_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899690.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8870..9805)
                     /locus_tag="ACG89C_001142"
     CDS             complement(8870..9805)
                     /locus_tag="ACG89C_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899691.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(10121..11770)
                     /locus_tag="ACG89C_001143"
     CDS             complement(10121..11770)
                     /locus_tag="ACG89C_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4899692.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11887..14490)
                     /locus_tag="ACG89C_001144"
     CDS             complement(11887..14490)
                     /locus_tag="ACG89C_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4899693.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(14502..15551)
                     /locus_tag="ACG89C_001145"
     CDS             complement(14502..15551)
                     /locus_tag="ACG89C_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4899694.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(15577..15891)
                     /locus_tag="ACG89C_001146"
     CDS             complement(15577..15891)
                     /locus_tag="ACG89C_001146"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS4899695.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15907..16353)
                     /locus_tag="ACG89C_001147"
     CDS             complement(15907..16353)
                     /locus_tag="ACG89C_001147"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4899696.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(16354..18276)
                     /locus_tag="ACG89C_001148"
     CDS             complement(16354..18276)
                     /locus_tag="ACG89C_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4899697.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(18488..19384)
                     /locus_tag="ACG89C_001149"
     CDS             complement(18488..19384)
                     /locus_tag="ACG89C_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4899698.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(19377..20057)
                     /locus_tag="ACG89C_001150"
     CDS             complement(19377..20057)
                     /locus_tag="ACG89C_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4899699.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(20058..20840)
                     /locus_tag="ACG89C_001151"
     CDS             complement(20058..20840)
                     /locus_tag="ACG89C_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4899700.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(20833..21750)
                     /locus_tag="ACG89C_001152"
     CDS             complement(20833..21750)
                     /locus_tag="ACG89C_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4899701.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(21914..22168)
                     /locus_tag="ACG89C_001153"
     CDS             complement(21914..22168)
                     /locus_tag="ACG89C_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899702.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         6854 a         4655 c         3763 g         7581 t
ORIGIN      
        1 ggtttgtctt gtgaaaaata atgattatcc ttaccaaaaa atgtttgttt ttctacatgt
       61 tcgtacttat caaaaaatat gatccttatt gaaattactg gctatttata ttccaacgac
      121 ttcttgtgtt aatttttgtt atctcatagt aaattatgtt tcattttcac ttaatttagt
      181 tgtcacgtgt tcgtcaattc gtaaaaacgg taaatatata aacacacttg cggctataat
      241 taatatttgt aaaacagctg ctcgccagtc tccggctgtt gctaaaaatc ccgatataac
      301 tggtggcgta gtccaaggaa tttgcactac tacatgatta attaatccca cagcagttgc
      361 aaaatatgct actgtcaaag aaaaaatcgg tgcaagcaca aagggaataa tcagactgat
      421 attaaataca ataggcaatc caaaaataat aggttcgctt atattgaata tagcgggagt
      481 taccgataat ttagcaaagt ccttataatc ttttctacga ctgaagataa atattgcaat
      541 caataaagca atcgtatttc ccgatccacc cattaccgca aaggtatctt taaaggccat
      601 attgataata tgtggaattt gctctttgtt tgcataagct gccatatttt cttgcatatt
      661 ctgaatcaaa aacggatcta acaatgaacc tgatattact gcttgatgga taccaaatcc
      721 aaaaaataga ttagcaatag atgtaattaa caaaaatcct ggtaaaccag ttgtaatttt
      781 acttaatggc tctgtaacta tcgtggtaat tagcttattt atatccatat taaacaattg
      841 aatgattata aatgacaata aagcaaacga ggtaatcgta ataatgattg ggattaatac
      901 attaaaagac tttaaaacag cgggaggaat atttccggat agctgaatct gtagtttctt
      961 attattagaa agtttaataa ataattctgt agccgctaat ccaacaaata tacctacaaa
     1021 cattcctgaa gctcctgtat aggatatagg tatcacaccc gtcacttcaa cagctttatc
     1081 cgcattttca ggagtaaaca tcatctgcat aggtgttaat acaacaaatg tcgaaatagc
     1141 aactaatacc gctgcaatat gatttgtata atttctgctt gcacacatat gataagatac
     1201 tgctgctaca actaataatg tagttatgct taatgtacca ttggcaattg aagtaccaat
     1261 ttgttgtaac gtggtcaatg tactagtatc gacaatttcc cccatgaatc ctgttggttc
     1321 taagatcact gaattaataa gtgtcacaaa tcctgctaac atcataaatg gcataaatgt
     1381 agcaaacgca tctcgcaaag ttcttaaatg gatctgattc cccaacttag tcgcaactat
     1441 accaaatttt tcaaatacaa tgtccttcct gctacttttg cccatatttt ttctccttta
     1501 tgcttattgt aatcgattac tttttccacc caaataatac atcacttatc aaaaaaagta
     1561 aatactttta attaaatata tatatctttt ttagtataaa aaaataagct ctgaaaaaat
     1621 aattttcccg agcttatttt tcaacaaaaa cagcttcaaa aacaaagtct tgatataaat
     1681 gtctcgatac cgaatactcg aaaggtttac cagacgatag agcaacaact ttgtctatct
     1741 caatcataaa ttcacctgga tctattttta atatttttgc gtcaaattca ttcgcagtat
     1801 ctcctttgat acgtatgtgc gaagtcttta tttccagacc cagttcattt gtgatataag
     1861 catatacaga tttctttaaa tgttttggtt ccaagcctgg tatgactgct aatggcatga
     1921 atgtttgctc aatcgagtat ggttgacaat caataaaacg gcttcgtaca atattatata
     1981 catagtcttt tacactgata cttaatttat cagctacttt tttcgatgga agttcaattg
     2041 agaaagtatg gatttctgaa tctacgtgtt cttcaccaac cacgctaaac aaaccttcgt
     2101 ttggaccaag aactcgtgag ttatcaattg gtgttctgac gacttcagtc ccatacccac
     2161 ttctacttcg caaaactcct tcggaaacaa gtaagtctac gcctttcttt actgttaact
     2221 tgcttgtatg ataaataact gccaattcat ctcctgtagg aagtttctca ccaattttat
     2281 actctccatt tacaattttt ttctttatat ctaagtaaat ttccatatac ttcgcaattt
     2341 ttttcaatgg tgaccctcca atcaaaaaca tatcttcatc tatactttaa cataaataaa
     2401 ccgataaaat ccacatcaaa attatacaca caataaaaaa gatatatata tttaaacaaa
     2461 actatttact ttaagttgta agcggtgtta tcattaagtg gaaacaggag gtaaaaaaat
     2521 tatgacaact atgaaaaagc taacttttcc agatgatttt tggtggggtt ccgcttggtc
     2581 agctgagcaa gcagaaggaa aaggagaaac aggaaaagca gagactatct gggatcgctg
     2641 gtttaaagaa caaccttacc ggttttatca acgaatcgat tcctctatta caaccgatca
     2701 catccatcaa tataaagaag atatagaact aatgaaaaaa actgggcata actcctttcg
     2761 cgtttctatt tcttgggcac gaatgtttcc aaatggcggt accggaaaag taaatcccaa
     2821 agccatttct ttctatcgaa aattattttc cgaaatgaat aataacggaa tcaaagtatt
     2881 tgccaactta tatcattttg acatgcctgc tactcttcaa gataaaggag gatgggaatc
     2941 acgtgaagta gttgatgctt atgttcatta cgctgaaact tgttttaaag aatttggtga
     3001 cttagtttgt cattggttta ccttcaatga accacttggg cctattttag gtacttatct
     3061 tgaagatttt cattatccaa acctcattga ctttaaacgc ggagcgcaag cagcattctt
     3121 cactatactt gcacacgcaa aagcaataca agtatttaaa gaactagctc ttcctagtaa
     3181 aataggcgtt attctaaatt tgagtcctac ttttcctcgt agtcaaaatc ctgctgatgt
     3241 gaaagcagca gaaatcgctg atttatttta tacgcggagc tttttagatc ctatggttaa
     3301 agggaatttt tctaaaaagc ttatcctgtt attacaagaa tataatcagc ttcctgaaga
     3361 ttatacgaaa gaagatcttg tatgtatcta cgaaaatacc gctcaaattc ttggactaaa
     3421 ctattacgag ccacgtcgcg tcaaatctcg ttcttctatg gtggatacat ctagtccatt
     3481 tttaccagag tggtttttcg aaccacatat catgcctgga cgacgaatga acgaataccg
     3541 gggatgggaa atttacgaaa aaggaattta tgatttgtgc atggatatca agaaccatta
     3601 tggaaacatc gaagcattta tctcggagaa cggaatgggc gttgcggatg aagaacgttt
     3661 catggatgat acagggcaaa taattgatga ttatcggatt gagtatatca aagatcatct
     3721 tgcttactta tggaaggcaa taaatgaagg atgtaacatt aaggggtatc atctttggac
     3781 atttattgat tgctggtcat ggatcaacgc ctataaaaac cgatatgggc tggtttcact
     3841 cgaccttact actcaaaaaa gaacaattaa aaaaagtggc gaattttaca agcaattaag
     3901 tgatacaaat ggatttagat acgatgtaga cttgttagtt gaataatatt ctaaaattcc
     3961 acttgaagtg aacacattag aaaacaagaa taatgagaaa taccaataac aaatactttt
     4021 tcttcatact tcatttatac ttgtcagtat aagagactgt gacaaatgta gtgatcctca
     4081 gaagttaggt ctggaaatct aacttgagaa gattttttta aactacgtcc agtctttttt
     4141 atttgtttaa aattgaagtt gacaagtctt cattaaaaat ttagaatatt cacaagtttg
     4201 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     4261 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt tggttatatc ggtattggac
     4321 ttgcatttgg tatcgttggg aaagctgccg ggtttcatcc gctggtcgtc acgttgatgt
     4381 ccctgctgat ctatgctggt tctgcccaat ttatcacggt cagcatgctt gctagccata
     4441 gtccactgct ctccatcgtt ctctcgacct ttctagtcaa ttcccgaatg attctgatga
     4501 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     4561 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     4621 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     4681 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     4741 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     4801 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     4861 gactgatttt tatcccaagt aacttagtga ttttagttgt aacactgatt gggtgcggat
     4921 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     4981 actttagcaa cttggctttc taggatccta ccatttgtct tactgaaaaa attcgatttg
     5041 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     5101 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     5161 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     5221 ttggtaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     5281 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     5341 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaat atatgtcctg
     5401 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     5461 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     5521 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     5581 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     5641 ttcccacatg tccaaatggt cggacaaggt gtttgatttg atcaaagtag cgggtaatat
     5701 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     5761 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     5821 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     5881 aaccgacgcc cccagctccg ttgatcacta agattttttt cccgcggttg gcattttctt
     5941 ctggagtcag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     6001 aggctgcggc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     6061 ccagttggta ttcttgattg cttcctgctc ttgtcgttgt acctgcgtaa aaaacgcgat
     6121 cgccgacaga aaatttttgc acttcatcac ctacagcaac aactttcccg actccatcaa
     6181 accctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     6241 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     6301 gttctttttc atcaataaag ctttttggat catcgatcga caacccttca taaaaaccga
     6361 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     6421 catccataca gttttcattt caatatatat agtatgctat ttgttccact tttattcatc
     6481 ataattattc tcttacttaa agtaaactat ttgcactttt ttggttaaaa aagaggttgc
     6541 gacaagactt ttgtcacaac ctcttttaaa cggtgttcaa tcaactgacc ctcggtattc
     6601 gctcaaaaaa cgacaaaata tgaggagcta tttttgtttg gctgttctta ttacttgatg
     6661 caggacagct ttttcacgac ctctaattca cggtgttcaa gcaagaaatc attgttcact
     6721 gaaaagcgtt ggatgttctt tttcaaaggt gtctaagatc tctcgtactt catcagtaga
     6781 tttcgtattc atcaactgaa ctcgcaagtc atttgctcct ggaaatcctt tgatatagat
     6841 tttaaagaaa cgatgtagtc ctgtgatgga acgcgggatg atttctgaat aatgatcttg
     6901 cagatctagt tgcagacgaa gtagtccgat cagttctttt tctgtatgtt ccctcggttc
     6961 tttttcaaat gcataaggat tcttgaaaat cccccggcca atcatgatgc catctacgcc
     7021 atatttttcg gccaattctt ctcctgtctg gcgatctggg atatctccgt tgatcgtgat
     7081 cagtgtttgc ggagcgattt ggtcacgtaa agcaatgatt ttagggatga cttcccaatg
     7141 tgcatctact ttactcatct ctttacgcgt acgcaaatgg acagaaagat tggctatatc
     7201 ttgatgaagt aggtgactga tccattcttc catctcagac atttccgtat agccgatacg
     7261 cgtcttgacg ctgacaggca aaccgccagc tttagccgct tcaatcaatt gtgccgctac
     7321 ttctgggcga aggatcaagc cactgccttt tccacgttct gctacattag ggactgggca
     7381 gcccatgttg atatcgattc cttggaagcc catttctgcc atagcaatgc tcatttctct
     7441 aaaaaattct ggcttgtctc cccaaatatg ggcaaccatc ggctgttcat cttctgtaaa
     7501 ggtcaatctt cctcgtacac tgtcttttcc atctggatga cagaaactgt ctgagtttgt
     7561 aaactcagta aagaagacat ccggtgctgc tgcatgcttt acgacatggc gaaatacaac
     7621 atctgttaca tcttccattg gtgctaaaac aaaaaaaggc tttggcagct ctttccaaaa
     7681 attagtagtc atttatttgt tactctccat tctatcaagc agtgaagtcg caatctttct
     7741 caaaaacatt gtcattatac taggaaacaa aaaaagaagc aaagtgaaca gcttaaaaga
     7801 tgtttctgat tgagcaaaaa aagactgtga caaaaatctt atcacaattc cttttccgga
     7861 ataaatggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaaacg aaaaatagga
     7921 ggagctattt ttgtttggct gttcttatta cttgatgcag aacagctttt tcacaacctc
     7981 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac gacaaaatat
     8041 gaggaactat tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
     8101 tcttttctaa cttctcaatc ctcttccttt attgatcaaa taccaagtaa cggcgtacaa
     8161 aacaacgatg aatagcacaa gtacgatcat cgatgttgct actggcacat cgattgttcc
     8221 aaggaatccg taacggaaac cggagatcat ataaacgatc ggattgactt tcgaaacagc
     8281 ttgccaaaat ggcggcaaca tagagatggc atagaaaacg ccacctagat aagttaacgg
     8341 ctgcaagaca aatgtaggca cgatcgatac atcgtcataa gactgagcaa aaatcccgtt
     8401 gagcagtcct gctaacgaaa acaagattgc cgtcatcaat aatgtaatga tgaccatgaa
     8461 ccatgaatac acatgaagtg gtacaaagaa caaggaaatg atcgttacga gagagccgac
     8521 gagaatactg cgacccactc cgccaatcac aaagccccaa atgatcacat gcgtaggaac
     8581 aggcgctacc aagatttctt cgatgttttt ctggaatttt tgagagaaga aggacgaaga
     8641 aacgttggca tacgaactag tgatagcaga catcataatc aaaccaggca cgatgaattc
     8701 catatatgaa aaaccaccca tgtcaccgat ccgaccaccg atcatttttc cgaaaataac
     8761 aaagtacaag gatgtcgtga tcacaggcgg aaccagtgtt tggacccaga tacgcaaata
     8821 gcggttcgtt tccttgatag ctaaactttt taatgcagta aaataaagat taaacatttt
     8881 tctcctccgt atgattattt tcttctgtga ttttcaaaaa cagttcttct aaacgattcg
     8941 acttattcct catagaaaga actttgattc cttgtcttgt cagctgatcg aagattccgt
     9001 tcaccccttg atttcgttca acttcgacag cgattgtctg ttcatcttca aatgtacaag
     9061 cgtatccttc aatcacaggt ttggtctcat atggcgctaa atcaaagata aatgtttcaa
     9121 attgcagttt ggatagaagc tctttcatac tagtattctc gatcagttca cctgattgga
     9181 tgatcccgat gttgcgacaa agcatttcag cttcttctaa ataatgcgtc gtcaaaatga
     9241 tcgttgtacc attttcgttc aattctcgga gaaactccca catctcgcgt ctcaattcaa
     9301 tatcgactcc tgctgttggt tcatccaaga tcagcaaacg tggttcatgc atcaatgccc
     9361 tagcaatcat cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg acatttctct
     9421 tttcccaaag attggactgc ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag
     9481 agacaccgta gtagcctgct tgattcacga cgatttgctg gaccgtttca aagggattga
     9541 aattgaactc ttgcgggact aatccaatct gttgctttgc tcggactaga tccgtatcca
     9601 gatcataacc gaagacattc acttttccag atgttttatt gactaaagaa gtaatgattc
     9661 cgatcgttgt agacttccca gctccatttg gtcctaatag cgcatagaaa tctccttctt
     9721 tcacggaaag atcgattcct cgcaatgctt cgaccccact tccataaacc tttttcaaat
     9781 tatttatttc aagcgcattt gccatttatt tcctatctcc ttatagacat gattctttat
     9841 tgtatcatgg aatacagcag atgaaaaaac aacttggttc cagcgaaaaa tcaggttgct
     9901 gggctcagga aaatctccta atgaaagaca tcaatcggac gaagtcagac aaccttttca
     9961 gaaaaaacaa atcgaagata aaggattact attcatatgt gcaacaaaag gagcgtgaga
    10021 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcaaaag
    10081 ccaagatttt tgatccaata aatactttcg atcacttcgc ctatcccaat acatgccact
    10141 gccgcttttc atcaaataac gcggcttccg tttttgcggt ttctactaaa gtctctgggt
    10201 aatccaaaac tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt
    10261 tgaaatcaaa actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac
    10321 aataattctt taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg
    10381 gatactccgc aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg
    10441 tcccttttaa aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca
    10501 acaaacgttt gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat
    10561 cttcgattgc catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg
    10621 ctgtttggat cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt
    10681 ttcctgatgc attggaaccc gtgactaatg tattcttctg ccagttgacc ggattcacta
    10741 ccgcttcttt taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact
    10801 ctggtcggca agtgatgggc atataagtac ggtaattcaa tattgcagcg gctacttcta
    10861 attctcccag caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga
    10921 gaacgaaatg ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca
    10981 actctgcttc actcccactc ttcatacgaa atgaaaaacc taatttaggg atgtctctta
    11041 atggtttagt ttgctgtttc agctcttctt gaagtggtgt cggcagtttt gctagctgat
    11101 tggcggctga aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt
    11161 gtttcttcac tagataatag accgtattga atactaaccc accgattgcc aaatagattc
    11221 ctagtacttg tcctaaaaga agcaaaccca aacccaccag cggaagcaag ccaagaaaaa
    11281 taaataaacc tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa
    11341 aattatgatc tttttttcct aaacgagcga attgatattg gattttttct ctcagttgag
    11401 gattttcctg ataaaaagca atcagttttt ctaactgctg atcttcacca aaatcaaagc
    11461 tgcgtaaacg ttgatacaac gcttctgaac ctacactaga ataggtactg ttgattcctt
    11521 cgaatacttc gaacatatcc aggtcatacc aagtaagatc atcgatttcg ctatcccatg
    11581 aacggaactt cttctccgtg agccatgctt ccttcaagct ttcttcttta tcaaatctcg
    11641 gttgataagg aattttttcc catttgctac gaactgtttc ctttaatttc aaacgtgaaa
    11701 aaatgtctaa agcaactaat aaaataacag ctgcaataat aattaaaacg ataaattctc
    11761 tttgacccat atccctctcc gttcctttat ttgttagctt tagtatagca ctgttgcgct
    11821 atttgaaaag agggtaaaaa aacacctttc aaaataaaag gtgttttttt cttgtatatt
    11881 acatatttat ctctcattta tcaaaattga caaataatca tagggaggaa ttgttgttaa
    11941 ttcttgttta ttctccagtg cattgacaac tgtcccaagg gaaaggatag cctcaggtac
    12001 tattttttct tgttctgttg gattagctaa gcgaagaaca aatccttgtt catgaaaata
    12061 agaaggataa atcgatgata caattaagtc ttcaggtaat tcaatcaatg aaaatttacg
    12121 aggaacatca agttcatttt gtaatggcca aatcttgtta tctaacctgt gaataaatac
    12181 attcagtttc tgcttttgat aagaaataga ttgttctaac cgtttttctg cctgttttgc
    12241 cactttgaac tcattaaatc ttccttcttc tacattaaat ccaaaagtga ctttatactc
    12301 acctatctct tgagctagcg gtgtttccat catgacatgc ccttcatttg tcgtatctcc
    12361 tgaagctcgc ccaggtcgcc aagcaagatt aggttttcca agttcccctg ttgttgaaaa
    12421 taaggttatg attaattgat tttctatacg ttcatactct tttaaaccat ctgcataaaa
    12481 cgtaaaataa tgatcatttt ctgccacaga gacactctta tcgaatattt ctaaattaac
    12541 aggtttctct accatagttt ctgaccaatt cggaggaatg acatcagggg tattttcaat
    12601 aaatccgttt tgaatttgtg caattgattt ttcagattta tctaaaacat taattgctaa
    12661 gcgcatacga tgcgagtaaa cagtattatg aattttgagt gtgccttcaa cgatttgact
    12721 ttctcccaga ctgaacgata tttcataaga tatttcttcc atttcgggtt tttcagataa
    12781 acgatctttt aaatctttag gaagttgagc ttttccgtaa acaactaatg tttcttgtaa
    12841 agaatcttta tatactttag ctgtttctaa ttttagagat agttctgtat ctccttctaa
    12901 tggtgaataa tcatacgtat cgccatcatt tgctgaatcg atcaaatgaa cgaaatcaag
    12961 atattgtcta tctccaacta tcaaattcaa ttgcccatct ttaaaagaaa gtttatattt
    13021 attgttactt atctcagttt tgtctatctc gattgctgtt tctaattttt cattattttt
    13081 ctcaaattca acaacagtgt atcctagtgc tggcacattt acatttattt ttatgtcaag
    13141 ttcataataa gctggttcat caaaatattc gaaacctgta gcaatcatcc gataagcatt
    13201 tttccgttct ggaaaataaa cttctttttc aatgatatta tagggactac ctttgaagtg
    13261 tattttcttt gaatcagcta ctacatgcac gattttcgat ccactataat tctcagtcgt
    13321 tgtgttgaaa acaaggactt cattcgattt tagctttaat gcatcagcca tccgttttac
    13381 aataaggttt tcgattccgt cagctatctc attagcttct ttgattcgat ggaaaatatc
    13441 ttccgcaaca ttatctgaaa cacagccacc aatactatca tgagcctggt tttgcaaaat
    13501 ctttttccat aaccgacgaa gtaggccttt actgatatgg ataccatttc tttctgcaat
    13561 cagcatgagc ggctcaactc gttttagaat tttttgttcc aatttaaaat tttctatttt
    13621 catttttgtt cttactgagc caatagaacg atgaacacga gagtaaaccg gctgacgaag
    13681 ttcaccttta tattcaggta attctttttt gtttctgata acttctacat aagaaggata
    13741 atctgaaata atattgtgga atttactgta tttattcaac ttctctacga cttgatcaaa
    13801 atttaaaatc attgactttt gatctacacc cgaaggtact aaaatatctt tgttttttcc
    13861 ttgcatatga agcatttcaa ctgcttcatc caacttttct tttgcaaatt caggaatatc
    13921 tttaattgcg tctaaggtca tcattccagt catataacca aatggaaaat tcatggcata
    13981 aacaaattta tctcccaatc ctttccagat aaaataagga gatggaacca tcgtttcaaa
    14041 gtttaatcct cgccaacaca tgaaattatc gatttctact tgatgcaaaa gtgtaggcaa
    14101 attcgcattg aacccaaacg tgtcaggcaa ataccctacc atcattggat ctccatattt
    14161 aaggatcgta tcactgattc caatcattaa gtttcttaaa attgattcag catccactaa
    14221 taaggcatct gtttgtgcat accacggtcc cacgaatagt tgtcctgttt tcatcagtcg
    14281 ttggataacc ttaagctttt gcggattgat ctccaaatac tcatcgacga ttgaagtttg
    14341 tccatctaaa caaaagttta ctttagggtt cctttccaat tcatctagaa cttcagtaaa
    14401 gacctgatca gacaatacga tggcatcttc atttgtaaaa taccattctc tgtcccagtg
    14461 cgtgtgttga attatatgtg catttaccat atcagattct cctactttcc ttcttctact
    14521 gaatttactt gttgttcttt tttatacttg atggatttta gtgcattgac cattaacgct
    14581 gtaaccactc ccccaataac aattgatact aaatataaag gtatattcgt aacaaaagga
    14641 attgccataa aggttgaaag cattgtttct tgagtaacac caaaaaatcc atttagacca
    14701 catgctactg cagtgcccac cacgatgctt ggtattactc gtaaaggatc tttcacggca
    14761 aacgggatcg tcccttcagt aatccccaca aggcccataa tcatagctgt ctttgcatta
    14821 tctcgttccg attcatcata aagttttttg tttaaaattg ttgctaaaaa cattccaaat
    14881 ggcggaatag caattcctaa taacatggct gtactaggtc caaagcttcc gcttgaaaaa
    14941 gaagcaaccc caaaagcata tgcgatttta ttgataggtc ctcccatatc aaacgccatc
    15001 attcctccta aaatagccga catcaaagca gcatttgctc ctgacatgcc ttctagccaa
    15061 ttagtcagtc ctgttgtcat tgcttgaata ggcgtagcta gtacatacca taatatgatt
    15121 ccaacaacta atgtagtaac aaaaggaatc agcataattg attttagact aataacttgc
    15181 atcggtactt ttactttttt caatgcatta gctaaatagc cacaagctaa tccagcaacc
    15241 attcctccaa taaatcccat accaagattt tgacacatca tcccagtgat aaaagctggt
    15301 gctagaccgg gacggtcagc aattgaatat gcgatatagg ctgaaatcat cggtacgata
    15361 aactgtaatc ccgtctgacc aatcattcgc aggactcccc agaatgtatc cgtctggtcc
    15421 caaacacctg tgccaccaaa aatgactgca atagaagtta gtaaccctgc tgctacgaca
    15481 agcgggatca tataggaaac cccactttgg aaatgattta gaatttctct tccttgtttc
    15541 ttgaattcca ttagtaactc ccccacgata tattttttat ttgttaatta atgcaattgc
    15601 ttcatcaatc gtctcttcac catcttttac tacctctgag gtccctactg ttaaaatcgg
    15661 tttaccgtca aagcgacctc gttctgaaac atttgtatct actgcaaata tgacaacatc
    15721 tgcctcggaa acatccgaaa ttgaaagttt gttttctatc cccatagccc cctgtgtttc
    15781 tactttaata tctaaccctt tcttcttcgc aaacttttct aaattagctt ttgccatata
    15841 agtatgggca atccctgcga tacatgctgt tactgctact atttttgtca tatgaaattc
    15901 ctccttttaa aatgatagag cttttaccaa ttctttttta tttctcgaat cttttattct
    15961 tttaacaaac tcgtcatcca tcaatcttcc agataattct gctaaaatac gtaaatgatt
    16021 agtaccttta tcagtgtctt ttattgctaa caaaaagacg agatcaactg gttgatcatc
    16081 taatgcgccc cattcaacag accgctttaa cttggctaca gccactgttg attcaattac
    16141 tgctaggctt ttgccatgag gaatggcgat atttttccca attcctgtgg tcatatggtt
    16201 ttctctgtca aatacgtctt tttgaaattg tttgtggtca gacacaaacc cattagtctc
    16261 taacaatttc gataattttt cgactacact ttttttatca gatgcattta aatctatcac
    16321 tatattattt atattgattt tttcactgat catttataag ccttctttca atatataaac
    16381 taatttttca aacgacgaac tcgtttttaa atttgctaat aactcatgat tcgaagcgat
    16441 ttcattgaat gtttcataaa ttttattcaa ttcaggtttt actttttcat taagtgcaaa
    16501 aaagaaaaca gaatgaacaa gtgtcccatc ccaatcaatt ccttctggat taacgtagac
    16561 acagattact ggctttttta taaaatctac ttcgatatgc ggcatagcaa aatcattcat
    16621 cgctgttgaa gatagttttt ctcttttgat tgctccctct agtatccctt ctttcgcgta
    16681 attttctttt accaattttt tggtaattat atttaaaacg ttcacttgat tatcctttat
    16741 ttggtgggta aaaaagcaaa atcgtttatc taataaattt agaaaagcat cttctgtttg
    16801 ttcatttaac tgtagatact gttttacttt tctgacatct gcccctaaca tcaaaggact
    16861 aacaacaata acaggaaaat ttgtattttc aatcggaatc gtgcttataa caagttcatc
    16921 agtgactttt ctatcttgta gttcattgat gcttagtaca tcttctatct caaccatatc
    16981 tgcaaaaccg ttatttattc tttgctctaa taattttgat gttccatatc cgctactaca
    17041 aactaatatc actttcgttt tgtcaggttt gtaacgatcc aaaagtgatt gaatatggag
    17101 tgttacgtaa gcgatttcat cttcattaaa atgtatacag aatctctctt cgatttcctc
    17161 taataaatct acacttatca taaatgacct tttaaaagaa tatttgattt tgtccgtata
    17221 agggttatag atatttactc ccaatttgag tcttttaact gctgcattga gatgtaatac
    17281 tagattattg atcaattctt catcaggata aaattcattt aatttaattc ttactaactc
    17341 ttgtagctca acacccatat ctaaatagga aagcttcgtt tgatctaagt cgtgattcat
    17401 agcatttttt tcaatcgaag taatgtgaat gtcaacataa gcttgttcat ctattggcac
    17461 aataattgaa aatgcttttt caatcatttt gattaaaata agtgtattct ttgagatttc
    17521 ttcttcagaa ttattactac gtgataagaa attgcttgtt tcaatccttt ctatcgcaat
    17581 agcaaggtgg atagctaaag tttgaaattc ataatctgta aatttcaatt gtgtcttatt
    17641 catgtatcga ttaagcaatt cggctacttt tttcaatatg tccttattta agaaattatt
    17701 cgcttcacca atgaaattga tattcaagtc taagagatct gttttactgt tagatgctgt
    17761 aaagccaccc caataatagt tcacaacttc tgacatcaac cttcttttac ttttttcatc
    17821 agctttgatg cgcacgccat ttcgatctga aactaaggac atcccatgct tgtttagtct
    17881 ttttctaaca tctttcattg tgctttctac cgtcgacctg ctcacgtata attcgtcagc
    17941 aagattttga atctttagag aatcattagt attcaataat ttagcaaaca cataagttag
    18001 acggtcctcc ggagagtctg cgatattgat tttatgactg gataataaat ttcgaagtgt
    18061 ttcttttttt ccaataagcg aaacgccttt tttaggttga accaacattt gtacatctgg
    18121 cattttttgg attatttctt tcaaaattaa aatatctttg ctaattgttt tcgtagatac
    18181 atataattgt tctgataatt ctttgtaagt tgtgtgctca ttttcaatca aaaagtatag
    18241 tattttcatt tgccgttcgc taaattcgat gcccattatc ttcacctcct gaatacagct
    18301 taagtgtaca attattatct ctattttcat acagaagaaa gttcttgatt aatgtagaag
    18361 tttctaatat tcttgattat ttcaaatcaa taaaaaagcc tgcaatattt atatcacagg
    18421 cttttttctc tattaaatat atataagata atacattttt ctactagttc tcactaatct
    18481 tgtataatta aaccctccgc aacttgacgg agatggagaa gatgcccccg tctaatctag
    18541 cggtcatctc tcctcccatt ccttccacca atttttttga caggtaaagc ccgattccgc
    18601 tagattccgt atttgtgcgg ctggtatttt cggtataaaa acgctggaag actttttcaa
    18661 cagaggtttg cgttcctggt ttgataggat tggccactgt taatcgaatg aatggatcct
    18721 cttttgaaaa tgtcaccttt gcttgtccat ctgcataacg caataaattc tgtatgatat
    18781 tttgcagcac tcgcagcaac atgagcggat ctgctaatac agttgtattt atcgagtgtt
    18841 cagcgaacgc tacctcgata tttttctcag aaaattgttc ataaaaagag aaaaatactt
    18901 cttccacctc tttagaaaaa gaaagcgtat ggagatccat ttctttttgc ttcgtctcta
    18961 ttcgagcaag atcataaaac tgatccgtca tactgagtaa tcttgcaaca gattgtttga
    19021 tgatcgttaa atacttctcc cgtttctccg tatcctctac ctgttccagt aattgtacat
    19081 aaccgttaat agcagtcaaa ggagttctga gatcatgaga aaggccgacg atcgatagtc
    19141 tgatgttttc ttccatttct tgaaatgctt gtcctttttg ttcattttca tccaaccatg
    19201 tattcagtgc atctactagg tccattagat tcttttctcg aaattcgact gaaagacgac
    19261 caccatattc ttgtcgttca gaaagagtgg cgattttctt agttaatctc ttgatttccc
    19321 tttgcaggaa gaaaataaaa cttcccataa agataacaac gagaagcaac agccatttaa
    19381 tcaaatctaa accccactcc ccacaccgta tgaatataca cagcatgtgc ctttgctagc
    19441 ttccctcgaa gattggaaat atgcatattc actgttttct cactatcaaa atatggttct
    19501 tgccaaactg tttcataaag atttgctcgc gaaaatactt ttttcggatg ttctaaaaaa
    19561 agcttcagca gatcgaattc tcttcctgtc aaatggacag ccacaccatt cacatttaca
    19621 tccctcgact ctttgtccag taagatttcc ttgtaataga tgcgttgcga cagatctttt
    19681 acttggacag aagtctgatg acgaagttgg atgccgattc gtaacaaaag ttcttcgata
    19741 tcaaatggtt tcgtcacata gtcatccgct cctgctgcca acaaggaaac tttatctctt
    19801 tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg atgtctgtcg gatttctgct
    19861 aacaatgctt cacctgaaag ccctggaagc atgagatcta gaaggatcaa atcgacagga
    19921 tatgtgctta acaataattt tccttccgta cctgaataag catcaagaag tttgtatctc
    19981 tgttcaagaa cctcctccag taaggaatga acagcttcat cgtcttcgat aatcaaaata
    20041 gtttccagca ggaccaccta cttaatgtca gatttttgga aaataaacat tcctagagct
    20101 gatgatacca cgattgttac tagactgacc agaatcattt tcggtgcttg cgaataagtt
    20161 gcttgttgcg caaagtccgt atacatgagc cacgtttccg taaaccactc cgttagaaat
    20221 tccaagcctt tgatttttgc gaaaacaccg ggaatgaagg ccgttcccat gatataaaga
    20281 aaagaaagaa gcaaactgac actggtactt ctagtgatga atgtcaaagc cactggaaca
    20341 gataatgtcc ctaatgtcag cagaagcaat acaggaaata cttttagaac ataatttcca
    20401 tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa gtgtgtaagc aagcatggct
    20461 agtcccgtga tcaaaaccac gccaagtgtt gtgatcaaaa gcgtaacgag aagtttcgac
    20521 acataataag tcattcgatt ccgtcctaaa gataacgtat tgcgaatcgt accgttttga
    20581 aattcttccc caatataaaa gcacgcaaat gctgcgaaag ccagaagaat ccatgcagcc
    20641 attttttcta ccattaaaat ccctgttgca ccgttgatga ctgtatccgt tgcttggttt
    20701 aattcattgc cgtattttgt atgcatatac cattcgttcc aagaataaaa aagcggaagt
    20761 acgaccatca gtcctagtga gacccacatt ttacggctct tccatacttt tattttttca
    20821 gcagttaata gattaaacat gtggattgcc tccagttaat tgtaagaaat aatcttccag
    20881 tttttgccca actaggtgaa taccatccac aggaactttt gcttctacca atgcttgatt
    20941 gatggctgct acttggtcaa ttccttcaaa aagctggata acacggctag attgtacaaa
    21001 ataatcccta taaccaagag agtcgagaac cgttactgct tgttccgtcg ccgaagtgtc
    21061 aatttttata aattgacgag attcttgtgc caattcttct ttcgataatt ctttgatcaa
    21121 gtgtccttca tgcaaaatac catagtgagt ggcgatctgt gacaattcat ctagcaagtg
    21181 gctagaaagc aagacggtga tgccccgttc ttttaccaaa cgttggatga tttctcgcat
    21241 ttctgccatc cctgctggat ccatcccatt cgtcggttca tccaaaatca aaaattctgg
    21301 attcgtgatc agtgcatttg caatagctaa acgctggcgc atacctaaag agaaattctt
    21361 tgctttcttt ttccctgtat tttccaatcg catcattttc agcaggtctt cgatttctct
    21421 atccgacaca ccgccgttcg ctgcttggat acgtaggtta tctcttgcag ttaattctgg
    21481 ataaagagca ggggtttcaa tcgattgacc catacgtcgt cttgctgcct gcaaatccgt
    21541 cgttccaaat aattcgatcg aaccttcatc aatcgagatc aaacccataa tagttctcat
    21601 aaaggtagac ttacccgctc cattttcccc aatcaatcca tagatcattc cacgtttgat
    21661 ttcgattgat actttatcta atgctttggc agcaccgtat tttttggtta taccagttgc
    21721 tttcaagacc gtttcatttc caaaatccat acttatcctt ctttcttatt gatgagtgaa
    21781 gtatagttga cgggtataaa gaaagcagcc agaaatgtat aaagaaagtg taaattttcc
    21841 aattgaatgt caaaaaaaac aatcggacaa tacgtgtctt tcctattatc aggcagtttg
    21901 ttatacgctt gtatcagttt cctgattctt tcttttcgac caaagtcgtt tctctgtatt
    21961 cctcttcact gaaaattttt gcttctttca gatcaagctt gagtaatttt accagaccgg
    22021 ttataaggat aactatagca agactccctc ccagaaaaat tagactgtct cgcagacgtg
    22081 gttgcgttgt cttccaatca aagagcgcac caaacagtgc taatccaagt ggactggcca
    22141 ccatggtaat cgttgttatc gttgacataa gtcgtccttg tatatgcgtt ggaacttctc
    22201 gctgaagcca gaccatataa aaagcaaaag aaaacattaa tccttaaaaa cggacttatg
    22261 aaaaataata catgccgttt gggatttaca attagctacc aaataagagg ttatgacaaa
    22321 agacttttca caatcccttc ttccgaataa acggtgttct cctgccaact cctcggtcac
    22381 aagtcacaat atttatcaaa catgagaagc aatttttgta aattccttct tatttcttga
    22441 agctgaccac ttctgtcaca acctcagaag cggataaata atattgtttg atgatacttt
    22501 atctttttcc cgtttgttta tagatcgaat gatattatag accaagaatc ctacctatta
    22561 acatagctaa aaacacaaaa aaggcacttt ccaaattgtg gaaagtgcca gaaaagttag
    22621 gattaaggta actgcgctca gcattgcttc gcccagtacg attctcgtaa ctcgtgaact
    22681 cgaacgataa tcgcaattaa cgttttgaga actgtgaagc tttacgggct ttcttaagac
    22741 ctggtttttt cttcgtatca ttgaataagt ttgaaaaacc ttgatttata aaggttttcc
    22801 cgtaaatata ttttattata tttgattaaa tttgaaatgt ttgtgttcta aaa
//
LOCUS       DAZUKR010000033        22589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000033 DAZUKR010000000
VERSION     DAZUKR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.033"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACG89C_001154"
     CDS             127..3654
                     /locus_tag="ACG89C_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4899703.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACG89C_001155"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACG89C_001155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS4899704.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACG89C_001156"
     CDS             7442..7783
                     /locus_tag="ACG89C_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4899705.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACG89C_001157"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACG89C_001157"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4899706.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACG89C_001158"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACG89C_001158"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4899707.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG89C_001159"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG89C_001159"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4899708.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACG89C_001160"
     CDS             12994..13731
                     /locus_tag="ACG89C_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4899709.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACG89C_001161"
     CDS             13745..14560
                     /locus_tag="ACG89C_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4899710.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACG89C_001162"
     CDS             14573..15220
                     /locus_tag="ACG89C_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4899711.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACG89C_001163"
     CDS             15236..15892
                     /locus_tag="ACG89C_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4899712.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACG89C_001164"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACG89C_001164"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4899713.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACG89C_001165"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACG89C_001165"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4899714.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACG89C_001166"
     CDS             18234..18752
                     /locus_tag="ACG89C_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4899715.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG89C_001167"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG89C_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS4899716.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="ACG89C_001168"
     CDS             complement(19639..20559)
                     /locus_tag="ACG89C_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS4899717.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20685..21737
                     /locus_tag="ACG89C_001169"
     CDS             20685..21737
                     /locus_tag="ACG89C_001169"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS4899718.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21847..>22589)
                     /gene="fabI"
                     /locus_tag="ACG89C_001170"
     CDS             complement(21847..>22589)
                     /gene="fabI"
                     /locus_tag="ACG89C_001170"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4899719.1"
                     /translation="GKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERMKKSLV
                     KLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNVSDISR
                     DGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAKASLEA
                     AIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVTIEEVG
                     NACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7522 a         3942 c         4815 g         6310 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttcctt
//
LOCUS       DAZUKR010000034        21988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000034 DAZUKR010000000
VERSION     DAZUKR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.034"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG89C_001171"
     CDS             <1..395
                     /locus_tag="ACG89C_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4899720.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACG89C_001172"
     CDS             398..1147
                     /locus_tag="ACG89C_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899721.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACG89C_001173"
     CDS             1159..1428
                     /locus_tag="ACG89C_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899722.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACG89C_001174"
     CDS             1433..1597
                     /locus_tag="ACG89C_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899723.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACG89C_001175"
     CDS             1590..1892
                     /locus_tag="ACG89C_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899724.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACG89C_001176"
     CDS             1889..2050
                     /locus_tag="ACG89C_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4899725.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACG89C_001177"
     CDS             2047..2352
                     /locus_tag="ACG89C_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899726.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACG89C_001178"
     CDS             2352..2708
                     /locus_tag="ACG89C_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4899727.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACG89C_001179"
     CDS             2668..2913
                     /locus_tag="ACG89C_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899728.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACG89C_001180"
     CDS             2910..3329
                     /locus_tag="ACG89C_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4899729.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACG89C_001181"
     CDS             3326..3883
                     /locus_tag="ACG89C_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4899730.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACG89C_001182"
     CDS             3880..4176
                     /locus_tag="ACG89C_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899731.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACG89C_001183"
     CDS             4253..4666
                     /locus_tag="ACG89C_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4899732.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACG89C_001184"
     CDS             5012..5386
                     /locus_tag="ACG89C_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899733.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACG89C_001185"
     CDS             5423..5590
                     /locus_tag="ACG89C_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899734.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACG89C_001186"
     CDS             5616..5960
                     /locus_tag="ACG89C_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4899735.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACG89C_001187"
     CDS             5965..6246
                     /locus_tag="ACG89C_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899736.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACG89C_001188"
     CDS             6349..6663
                     /locus_tag="ACG89C_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4899737.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACG89C_001189"
     CDS             6641..8335
                     /locus_tag="ACG89C_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4899738.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACG89C_001190"
     CDS             8355..9533
                     /locus_tag="ACG89C_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4899739.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACG89C_001191"
     CDS             9496..10182
                     /locus_tag="ACG89C_001191"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4899740.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACG89C_001192"
     CDS             10182..11342
                     /locus_tag="ACG89C_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4899741.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACG89C_001193"
     CDS             11352..12227
                     /locus_tag="ACG89C_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899742.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACG89C_001194"
     CDS             12224..12535
                     /locus_tag="ACG89C_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899743.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACG89C_001195"
     CDS             12525..12878
                     /locus_tag="ACG89C_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4899744.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACG89C_001196"
     CDS             12868..13269
                     /locus_tag="ACG89C_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899745.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACG89C_001197"
     CDS             13262..13666
                     /locus_tag="ACG89C_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899746.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACG89C_001198"
     CDS             13678..14286
                     /locus_tag="ACG89C_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4899747.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACG89C_001199"
     CDS             14306..14668
                     /locus_tag="ACG89C_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899748.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACG89C_001200"
     CDS             14671..14853
                     /locus_tag="ACG89C_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899749.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACG89C_001201"
     CDS             14870..18289
                     /locus_tag="ACG89C_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4899750.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACG89C_001202"
     CDS             18340..19077
                     /locus_tag="ACG89C_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4899751.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACG89C_001203"
     CDS             19087..21378
                     /locus_tag="ACG89C_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4899752.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACG89C_001204"
     CDS             21402..>21988
                     /locus_tag="ACG89C_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4899753.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAZUKR010000035        21911 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000035 DAZUKR010000000
VERSION     DAZUKR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.035"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG89C_001205"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG89C_001205"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4899754.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG89C_001206"
     CDS             complement(1172..1867)
                     /locus_tag="ACG89C_001206"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS4899755.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG89C_001207"
     CDS             complement(1880..2596)
                     /locus_tag="ACG89C_001207"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS4899756.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACG89C_001208"
     CDS             2926..3534
                     /locus_tag="ACG89C_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS4899757.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACG89C_001209"
     CDS             3859..4182
                     /locus_tag="ACG89C_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4899758.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACG89C_001210"
     CDS             4364..5734
                     /locus_tag="ACG89C_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS4899759.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACG89C_001211"
     CDS             complement(5864..7165)
                     /locus_tag="ACG89C_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4899760.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACG89C_001212"
     CDS             complement(7519..9510)
                     /locus_tag="ACG89C_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS4899761.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACG89C_001213"
     CDS             complement(9654..9920)
                     /locus_tag="ACG89C_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4899762.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG89C_001214"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG89C_001214"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4899763.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG89C_001215"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG89C_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4899764.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACG89C_001216"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACG89C_001216"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4899765.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACG89C_001217"
     CDS             complement(14080..14601)
                     /locus_tag="ACG89C_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS4899766.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACG89C_001218"
     CDS             complement(14604..15446)
                     /locus_tag="ACG89C_001218"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS4899767.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACG89C_001219"
     CDS             complement(15439..16164)
                     /locus_tag="ACG89C_001219"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS4899768.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACG89C_001220"
     CDS             16340..16636
                     /locus_tag="ACG89C_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899769.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACG89C_001221"
     CDS             17204..18796
                     /locus_tag="ACG89C_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4899770.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACG89C_001222"
     CDS             18809..19549
                     /locus_tag="ACG89C_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4899771.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACG89C_001223"
     CDS             19717..21066
                     /locus_tag="ACG89C_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4899772.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACG89C_001224"
     CDS             complement(21194..21784)
                     /locus_tag="ACG89C_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4899773.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa a
//
LOCUS       DAZUKR010000036        21882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000036 DAZUKR010000000
VERSION     DAZUKR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.036"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..1104)
                     /locus_tag="ACG89C_001225"
     CDS             complement(205..1104)
                     /locus_tag="ACG89C_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4899774.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="ACG89C_001226"
     CDS             complement(1114..1470)
                     /locus_tag="ACG89C_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4899775.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="ACG89C_001227"
     CDS             1825..2121
                     /locus_tag="ACG89C_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS4899776.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="ACG89C_001228"
     CDS             complement(2765..3718)
                     /locus_tag="ACG89C_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4899777.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="ACG89C_001229"
     CDS             complement(3741..4496)
                     /locus_tag="ACG89C_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899778.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="ACG89C_001230"
     CDS             complement(4493..5452)
                     /locus_tag="ACG89C_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4899779.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="ACG89C_001231"
     CDS             complement(5445..6395)
                     /locus_tag="ACG89C_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4899780.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="ACG89C_001232"
     CDS             6545..6997
                     /locus_tag="ACG89C_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS4899781.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACG89C_001233"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACG89C_001233"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS4899782.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACG89C_001234"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACG89C_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4899783.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="ACG89C_001235"
     CDS             complement(10549..11358)
                     /locus_tag="ACG89C_001235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4899784.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACG89C_001236"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACG89C_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS4899785.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACG89C_001237"
     CDS             complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACG89C_001237"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4899786.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="ACG89C_001238"
     CDS             complement(16002..17783)
                     /locus_tag="ACG89C_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4899787.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="ACG89C_001239"
     CDS             complement(17809..18723)
                     /locus_tag="ACG89C_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899788.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACG89C_001240"
     CDS             complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACG89C_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4899789.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="ACG89C_001241"
     CDS             complement(19712..20656)
                     /locus_tag="ACG89C_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899790.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="ACG89C_001242"
     CDS             complement(20657..21661)
                     /locus_tag="ACG89C_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4899791.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6131 a         4656 c         3866 g         7229 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg at
//
LOCUS       DAZUKR010000037        21309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000037 DAZUKR010000000
VERSION     DAZUKR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.037"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACG89C_001243"
     CDS             <1..808
                     /locus_tag="ACG89C_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4899792.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1161..2009)
                     /locus_tag="ACG89C_001244"
                     /pseudo
     CDS             complement(1161..2009)
                     /locus_tag="ACG89C_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2162..3274
                     /locus_tag="ACG89C_001245"
     CDS             2162..3274
                     /locus_tag="ACG89C_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4899793.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="ACG89C_001246"
     CDS             complement(3352..3645)
                     /locus_tag="ACG89C_001246"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS4899794.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="ACG89C_001247"
     CDS             3813..4127
                     /locus_tag="ACG89C_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS4899795.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="ACG89C_001248"
     CDS             4148..6127
                     /locus_tag="ACG89C_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4899796.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="ACG89C_001249"
     CDS             6140..6466
                     /locus_tag="ACG89C_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS4899797.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="ACG89C_001250"
     CDS             6482..7000
                     /locus_tag="ACG89C_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899798.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="ACG89C_001251"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="ACG89C_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4899799.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="ACG89C_001252"
     CDS             complement(8436..9941)
                     /locus_tag="ACG89C_001252"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS4899800.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="ACG89C_001253"
     CDS             complement(10033..10623)
                     /locus_tag="ACG89C_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4899801.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="ACG89C_001254"
     CDS             10802..12448
                     /locus_tag="ACG89C_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS4899802.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="ACG89C_001255"
     CDS             12497..13216
                     /locus_tag="ACG89C_001255"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4899803.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="ACG89C_001256"
     CDS             13695..14642
                     /locus_tag="ACG89C_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899804.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="ACG89C_001257"
     CDS             14654..15574
                     /locus_tag="ACG89C_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899805.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="ACG89C_001258"
     CDS             15615..17072
                     /locus_tag="ACG89C_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4899806.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="ACG89C_001259"
     CDS             17417..18031
                     /locus_tag="ACG89C_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4899807.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="ACG89C_001260"
     CDS             18024..19748
                     /locus_tag="ACG89C_001260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4899808.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="ACG89C_001261"
     CDS             19745..21196
                     /locus_tag="ACG89C_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4899809.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6869 a         3607 c         4318 g         6515 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaggg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgg
//
LOCUS       DAZUKR010000038        20485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000038 DAZUKR010000000
VERSION     DAZUKR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.038"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ACG89C_001262"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ACG89C_001262"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS4899810.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ACG89C_001263"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ACG89C_001263"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS4899811.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ACG89C_001264"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ACG89C_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS4899812.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ACG89C_001265"
     CDS             2857..3732
                     /locus_tag="ACG89C_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4899813.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG89C_001266"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG89C_001266"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4899814.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ACG89C_001267"
     CDS             4629..5063
                     /locus_tag="ACG89C_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4899815.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ACG89C_001268"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ACG89C_001268"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4899816.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ACG89C_001269"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ACG89C_001269"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4899817.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ACG89C_001270"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ACG89C_001270"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4899818.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ACG89C_001271"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ACG89C_001271"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS4899819.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ACG89C_001272"
     CDS             13334..14260
                     /locus_tag="ACG89C_001272"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS4899820.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ACG89C_001273"
     CDS             14442..15335
                     /locus_tag="ACG89C_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS4899821.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ACG89C_001274"
     CDS             15469..15594
                     /locus_tag="ACG89C_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS4899822.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ACG89C_001275"
     CDS             complement(15728..16360)
                     /locus_tag="ACG89C_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899823.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="ACG89C_001276"
     CDS             complement(16508..17050)
                     /locus_tag="ACG89C_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS4899824.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="ACG89C_001277"
     CDS             17185..17574
                     /locus_tag="ACG89C_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899825.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="ACG89C_001278"
     CDS             17578..18342
                     /locus_tag="ACG89C_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4899826.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="ACG89C_001279"
     CDS             18471..19418
                     /locus_tag="ACG89C_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4899827.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="ACG89C_001280"
     CDS             19548..20288
                     /locus_tag="ACG89C_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899828.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DAZUKR010000039        19890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000039 DAZUKR010000000
VERSION     DAZUKR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.039"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACG89C_001281"
     CDS             135..2606
                     /locus_tag="ACG89C_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4899829.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACG89C_001282"
     CDS             complement(2709..4445)
                     /locus_tag="ACG89C_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4899830.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACG89C_001283"
     CDS             complement(4442..5146)
                     /locus_tag="ACG89C_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4899831.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACG89C_001284"
     CDS             5277..5780
                     /locus_tag="ACG89C_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS4899832.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACG89C_001285"
     CDS             5740..6078
                     /locus_tag="ACG89C_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS4899833.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACG89C_001286"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACG89C_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4899834.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACG89C_001287"
     CDS             7629..8207
                     /locus_tag="ACG89C_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4899835.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACG89C_001288"
     CDS             8211..8732
                     /locus_tag="ACG89C_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4899836.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACG89C_001289"
     CDS             complement(8811..9365)
                     /locus_tag="ACG89C_001289"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4899837.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="ACG89C_001290"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="ACG89C_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4899838.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="ACG89C_001291"
     CDS             9905..10156
                     /locus_tag="ACG89C_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS4899839.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="ACG89C_001292"
     CDS             10281..11072
                     /locus_tag="ACG89C_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899840.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="ACG89C_001293"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="ACG89C_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4899841.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="ACG89C_001294"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="ACG89C_001294"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4899842.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="ACG89C_001295"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="ACG89C_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4899843.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13129..14265)
                     /locus_tag="ACG89C_001296"
     CDS             complement(13129..14265)
                     /locus_tag="ACG89C_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4899844.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14381..15295)
                     /locus_tag="ACG89C_001297"
     CDS             complement(14381..15295)
                     /locus_tag="ACG89C_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4899845.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15386..15814)
                     /locus_tag="ACG89C_001298"
     CDS             complement(15386..15814)
                     /locus_tag="ACG89C_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4899846.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15819..16193)
                     /locus_tag="ACG89C_001299"
     CDS             complement(15819..16193)
                     /locus_tag="ACG89C_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4899847.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16479..16664
                     /locus_tag="ACG89C_001300"
     CDS             16479..16664
                     /locus_tag="ACG89C_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899848.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16676..16924
                     /locus_tag="ACG89C_001301"
     CDS             16676..16924
                     /locus_tag="ACG89C_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899849.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            16955..17134
                     /locus_tag="ACG89C_001302"
     CDS             16955..17134
                     /locus_tag="ACG89C_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899850.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17177..17647)
                     /locus_tag="ACG89C_001303"
     CDS             complement(17177..17647)
                     /locus_tag="ACG89C_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899851.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17734..18432
                     /locus_tag="ACG89C_001304"
     CDS             17734..18432
                     /locus_tag="ACG89C_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS4899852.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18610..18951
                     /locus_tag="ACG89C_001305"
     CDS             18610..18951
                     /locus_tag="ACG89C_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899853.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            18944..19615
                     /locus_tag="ACG89C_001306"
     CDS             18944..19615
                     /locus_tag="ACG89C_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4899854.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            19621..>19890
                     /locus_tag="ACG89C_001307"
     CDS             19621..>19890
                     /locus_tag="ACG89C_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899855.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
BASE COUNT         6561 a         3473 c         3926 g         5930 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag actgagaaag
    13021 aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta acgatttcta
    13081 tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt acagtttttc
    13141 tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa tcttttcagt
    13201 gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag ttcctagttc
    13261 cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt
    13321 catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga aaggagttcc
    13381 tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct cattgtaaat
    13441 agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt ttgtagaatc
    13501 agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat ttataactac
    13561 tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg cttctccagg
    13621 cctcattcca gtcaaatata gccactggaa aaggagagaa tatcttttat tgtgtgaagt
    13681 tgtgaaattt actagtcttt tatattcatc atcttctaaa aacttatctt taatttttat
    13741 cgtttttgac tctcgtttat aatcaataac tacttcgttg ataggatttt tttctacata
    13801 gccttttttc atagcgtatg aaaaaagaag attcaatttg gatttaatta cactgacgta
    13861 cttatttgac aagtcatctt tatatatcat atcttcaaaa atattattta aatctactgt
    13921 attaatacca gaaacgatgt aagtctcagg tatctttttc tttatagtgt ttaagatatt
    13981 attcgtagga taatatgtag actccttaac ttgtcttttg taaataacta accattcttc
    14041 gacaagctca tgaaatgtta gatcaggctt ctgaagtgtt ttttcgttta attttatatc
    14101 aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt tatttttata
    14161 agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct cattatattt
    14221 atatactttt tttccgtttt tatcagtttt ggtttcaatc cacattgttt tcatctccta
    14281 tttgttgcta taataggaat ggataagtaa gccaattata gcaggtttat ttttcatcac
    14341 gtccacaaac tttggcgagg gagggggcgt gtttttttgt ttagtagcta cctttccatg
    14401 aagtatctgc gaagaacact tcaaatgttg ttccatcatt tttcacttca aaatatgctg
    14461 ttccttgtgc ggatttaccc ggttggatgg tttccgaaaa gtaatctcta ctagctaaat
    14521 cagatttaat atctgatgaa tcataaaatt ctaaataact tgaacttgca tcaaaaggtg
    14581 tagtccctgt gttttctaca agaaaatcta cctttgcgta aaataggcct tctggtttat
    14641 agaaatcatc accgtaatct tttgatacag actggacagt tacgttaata gagttaccat
    14701 tttcagaagt aaaaggaact gcttctccta tactatgtgc aactgatgcc gtctggggtt
    14761 ctgaagacga agttgtagtt gtagttgtag tagagcttga atctttggat tttccatctt
    14821 tatctaagaa agttcgttga ttataatata caacctgttc agtttttgga ctaacccaaa
    14881 ttaacattgt ttcatcatca gaatcagttg aataagtata ttggagcatc tctaattttt
    14941 ttatatcttt tgcactattt ccatgttcaa ttaattcaga gtgctcttta taataatctc
    15001 cttgtgcatc ctttgaaata gatacaatta attcatcccg tgtgatagca ttgtctaatt
    15061 ctttgtaaac aaaatcattt gtccatttac gtaacggtga accaagtgtc tgataaactt
    15121 gagttaaatc atcaccaact tttattttat gaacgtctga tgaagttagt ttttttgttt
    15181 catattcaga tgttgatgaa acagtatttt ctttagtgct agcagaatta ggactagtgg
    15241 agcatccgct taataccaag cctaaaaaaa gcaacccaaa aacaattttt ttcattttta
    15301 attcctcact tctatgatat gatttttttg taggatctta gaaataaggt ttttagtccg
    15361 tgttgcagca cggacttttt ttacattata taagaaagcg ataagcgctt tctggaagtc
    15421 cgtaaagatt ctttaattcc tcaattcgtt taggatattg atcattgtct tctttgtata
    15481 gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg cttctagatg
    15541 ttcttgttga tacatagtaa ctggataagc cacgatgaaa aatagcgtga cctaattcgt
    15601 gagcgcaaat gtagaatctt tcctcagagt ctctcagttc atcatttaag aagattattg
    15661 cacgacctct aatttcttga aactgtcctt tgggattctc gataaaagga acgtattgaa
    15721 ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat ttccgcttca
    15781 actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt gcctttttct
    15841 ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac gcgttggacc
    15901 tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc tagtaattta
    15961 tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct accgagtaaa
    16021 taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt actaggagtt
    16081 ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc caattgagtt
    16141 atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt cattttcgca
    16201 cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac aactaataaa
    16261 agtgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg ataactaata
    16321 aacacttcac ggtcggcaaa ctctgaaatg taaattacta ggcatttctg tgtcttattt
    16381 agctatgtct atatactaat aaaaatatta gttattgtca acgaatatta gctaattttt
    16441 tagcttacag attatttttt tagaaaggag ctatttttat gtctgagaat ttagacttaa
    16501 aaattcgagc ggagatgaga aaaagaagaa tgactttcaa agaactagct gcgcttatcg
    16561 gtatttcagg agcttattta tcagatattc taaacggcaa tcgtgatgga aagaaagcac
    16621 aacagcatat cgaaacagtg aaaaaaatat tggacatccg ataggggggt aggcgatgac
    16681 aaaactaaaa aaacaagatt ttgtaaaaaa atacaattat tctccatcta cttatcaacg
    16741 tcgaatgtcg gaactaaaaa atacagcaat tttctcagcg gcgtatgaac gggtcacagg
    16801 acaagaagtt tggatcaata cagaattata cgataaattt ttgtctttca aatcctataa
    16861 caggttacgc acaagaaagg taacacctaa agaatttatc gagaagcatt tagttgattt
    16921 ataaaaaaca gaacattttg agaggtgaag gttaatgggt aaattcaaca gagcattggt
    16981 attcagcgca ccgctaatca tctacgcttt aggactttgg ggaagcaggc aagcgttgat
    17041 aggaacgatt gtttacatgg tttggatttt tatggggctt gatgaagctg aagctgagta
    17101 cagagcgaaa aagccaaccg agggggctga ctaaaaatga atagaaaaga gaaactagaa
    17161 tgtatattac tattactcag tttaattctt tcactaattt ctctattggg aagtttgtat
    17221 ttttgatatc aacaattttg tggatttggg ttctatttag agtaaatatc atttttgttt
    17281 ggtgattaag gagtttatta aaattaattt tccctacttc aaatgcaagg taacaagata
    17341 tagcactttt aggaggaata tttagcggta cagcttcaga gtatattcta ttatcatcga
    17401 ctgatcctcc tttagcaatt aatttcttaa atggagtagc tgaccatctg cggtttaagt
    17461 tagcggatga aagttctaaa ttaactaaca tagctggttc tgttgaatag ttagaaatga
    17521 ttacttttgt ataaatcata tctgcagcga agtaagaagc attaagttca acttctagtt
    17581 gagggcgcat tccttttatt tttatccctg ttaaaaacgt actcaatata aaacttatta
    17641 ttgacatcca ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc accagttttt
    17701 aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat tttgaaaaat
    17761 aaagaagctg tgactacgag cttacaagtg gcagaaagct tcgagaaaaa acatcaacat
    17821 gttttaagag atattgatgc actaaaaaaa gatgtgtcca attttggaca gatgtttgta
    17881 gaaggtaatg aaccagattc atatggcaga aatcgacgag ttttcttcat tagtagagat
    17941 ggatttttct tgctggctat ggggtttaca ggaaagaaag ctatctactt caaacaaaaa
    18001 tacattgaag cattcaacga aatggaagat gttattcgca agaatactgt tcctcaaaca
    18061 attgaagata tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc
    18121 atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa gcaaaaagga
    18181 aatatgaaag ttatgggagt tctaggggga aaagaaagcc gagcttatga agaaatcagc
    18241 aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat cccaagatat
    18301 ggcgagttac ctcgtaagag attcgatgat gctgtttcat ttattgaaat gtggttgcca
    18361 gaaactgcga tccgtatgga aattgatcaa ctgaacagac aacagagact ttttggtgat
    18421 gacaatgaat agagctgaag cgctaagaat agggacggca attgctaatt gctggtggaa
    18481 atactataaa ccaatcatcc taagccaaca acatattgac aagcaaaaag catggcaaca
    18541 aataaaaaag tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga
    18601 ttttagcata tggaaaaaga actttccact ctagatcaat atttgattga tcctgattgg
    18661 ggcaagccga aaattgagga aacaagtggt cgaaaaatca gacgaaatct tttgacgaat
    18721 gaagaactag cttgggatca agatgattta ggcaaccatg taactatttg ggatcatgtt
    18781 tatcttatcc atctatcgaa gcattcgaat aaacctgaat atatttacgt catcgaagat
    18841 ggcttgattg atgcgctaga agagtacgac agagataact tgattgatat ctcttattac
    18901 ggaccaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg
    18961 gacaacgaac tttgaaaaac tttttagtcg taagttaaat aaaattctca agaaaaaagg
    19021 aaattttgat tatttatctt gggctcacgc gtgggagatt atgaaaaaga atgatccaca
    19081 ggcaacggta actattaatg agtacaaaca ctacagggtt gtttctggaa ctcatcaaga
    19141 ctttcttgtt gaggaatata aaccttttct tatggatgaa actgggactt atgtatctgt
    19201 ctcagtaacg gttaaaggac acacggaaac cgaattattt cctgttttag attatcgaaa
    19261 ccaaccagtt gttaaaccaa atgcaatgca aatcaataac tcattgaagc gatgctttgt
    19321 gaaagcattg gctctacacg gactgggatt atatgtattt caaggggaag atattccaac
    19381 accacctaga atcgatacaa agaaattaaa catgctagag acgattctag aagctttcaa
    19441 tgagcagatg ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg agcagacaga
    19501 taaattaggg ctcttagctg ataacgttga aactattgaa cagttaagct atgagcaatg
    19561 tgccttgatg gagcgagcaa tagcagctaa gagaaaagaa ttagataaga agtgatatga
    19621 gtgtttaaac cattaatcga ttcatattca gcggttctga aaaagttcaa aggaaaagac
    19681 ataagcgcaa ccatcaatga ggaagtgaac attgatcgac taaagacgat gtatgacggc
    19741 tacgatggtg atcgagtcat tgaaattcgt tttattgatc ctagacgttt caccgtacag
    19801 caacgaaact tcatctatgc gctgataggc gatattttta tcgatacagg catgccaacg
    19861 gacttctgga aggaattctt ctacttccgt
//
LOCUS       DAZUKR010000040        19821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000040 DAZUKR010000000
VERSION     DAZUKR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.040"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG89C_001308"
     CDS             6..305
                     /locus_tag="ACG89C_001308"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4899856.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG89C_001309"
     CDS             378..1739
                     /locus_tag="ACG89C_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4899857.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG89C_001310"
     CDS             1761..2021
                     /locus_tag="ACG89C_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899858.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG89C_001311"
     CDS             2035..2787
                     /locus_tag="ACG89C_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS4899859.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG89C_001312"
     CDS             complement(2881..3486)
                     /locus_tag="ACG89C_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4899860.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG89C_001313"
     CDS             complement(3539..4513)
                     /locus_tag="ACG89C_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS4899861.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG89C_001314"
     CDS             complement(4891..5610)
                     /locus_tag="ACG89C_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4899862.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG89C_001315"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG89C_001315"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4899863.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG89C_001316"
     CDS             7161..8558
                     /locus_tag="ACG89C_001316"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4899864.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG89C_001317"
     CDS             8639..9079
                     /locus_tag="ACG89C_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4899865.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG89C_001318"
     CDS             9227..9553
                     /locus_tag="ACG89C_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4899866.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG89C_001319"
     CDS             9554..10303
                     /locus_tag="ACG89C_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS4899867.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG89C_001320"
     CDS             complement(10383..10838)
                     /locus_tag="ACG89C_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4899868.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG89C_001321"
     CDS             complement(11265..11540)
                     /locus_tag="ACG89C_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899869.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG89C_001322"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG89C_001322"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4899870.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG89C_001323"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG89C_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4899871.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG89C_001324"
     CDS             14770..17436
                     /locus_tag="ACG89C_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4899872.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG89C_001325"
     CDS             17800..18552
                     /locus_tag="ACG89C_001325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4899873.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACG89C_001326"
     CDS             complement(18574..19728)
                     /locus_tag="ACG89C_001326"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS4899874.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6329 a         3472 c         4060 g         5960 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa atggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DAZUKR010000041        19530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000041 DAZUKR010000000
VERSION     DAZUKR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.041"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACG89C_001327"
     CDS             complement(<1..703)
                     /locus_tag="ACG89C_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899875.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACG89C_001328"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACG89C_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACG89C_001329"
     CDS             1758..2837
                     /locus_tag="ACG89C_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4899876.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACG89C_001330"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACG89C_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACG89C_001331"
     CDS             complement(3572..3799)
                     /locus_tag="ACG89C_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4899877.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACG89C_001332"
     CDS             complement(3902..6490)
                     /locus_tag="ACG89C_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS4899878.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACG89C_001333"
     CDS             complement(6510..7169)
                     /locus_tag="ACG89C_001333"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4899879.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG89C_001334"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG89C_001334"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4899880.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACG89C_001335"
     CDS             complement(7942..8808)
                     /locus_tag="ACG89C_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4899881.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACG89C_001336"
     CDS             8917..9558
                     /locus_tag="ACG89C_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4899882.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG89C_001337"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG89C_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS4899883.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACG89C_001338"
     CDS             complement(10999..11898)
                     /locus_tag="ACG89C_001338"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4899884.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACG89C_001339"
     CDS             complement(11900..12697)
                     /locus_tag="ACG89C_001339"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS4899885.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACG89C_001340"
     CDS             complement(12675..13379)
                     /locus_tag="ACG89C_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS4899886.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACG89C_001341"
     CDS             13510..14085
                     /locus_tag="ACG89C_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS4899887.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACG89C_001342"
     CDS             14203..14880
                     /locus_tag="ACG89C_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS4899888.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG89C_001343"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG89C_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS4899889.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACG89C_001344"
     CDS             complement(16943..17941)
                     /locus_tag="ACG89C_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS4899890.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACG89C_001345"
     CDS             complement(18167..18820)
                     /locus_tag="ACG89C_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS4899891.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            19254..>19530
                     /locus_tag="ACG89C_001346"
     CDS             19254..>19530
                     /locus_tag="ACG89C_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4899892.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT         5654 a         4208 c         3397 g         6271 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt tggctctata
    19141 ctttttgatg ttggtgaact tcaccaacat cttttttgta taaaaaaata tagaaaagac
    19201 accctgaaat cctttagaat taatttggct accaattcat aggaggattc aagatgtctc
    19261 atactcagct tatcaaagat accctaaata ttctagacct aaatatctat ttcgaggaaa
    19321 attgtttaac taaagaaaaa tacaaagggc aaatttgctt gatttacaaa ggaactcttc
    19381 tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt
    19441 gggggacgac aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg
    19501 agctgaagaa acaacgattt aaatgtaagt
//
LOCUS       DAZUKR010000042        19062 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000042 DAZUKR010000000
VERSION     DAZUKR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.042"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..1645)
                     /locus_tag="ACG89C_001347"
     CDS             complement(77..1645)
                     /locus_tag="ACG89C_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS4899893.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1649..1930)
                     /locus_tag="ACG89C_001348"
     CDS             complement(1649..1930)
                     /locus_tag="ACG89C_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4899894.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1927..2850)
                     /locus_tag="ACG89C_001349"
     CDS             complement(1927..2850)
                     /locus_tag="ACG89C_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS4899895.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2847..3725)
                     /locus_tag="ACG89C_001350"
     CDS             complement(2847..3725)
                     /locus_tag="ACG89C_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS4899896.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3712..5049)
                     /locus_tag="ACG89C_001351"
     CDS             complement(3712..5049)
                     /locus_tag="ACG89C_001351"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS4899897.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5052..6155)
                     /locus_tag="ACG89C_001352"
     CDS             complement(5052..6155)
                     /locus_tag="ACG89C_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4899898.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6229..7737)
                     /locus_tag="ACG89C_001353"
     CDS             complement(6229..7737)
                     /locus_tag="ACG89C_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4899899.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7703..8395)
                     /locus_tag="ACG89C_001354"
     CDS             complement(7703..8395)
                     /locus_tag="ACG89C_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4899900.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8417..9391)
                     /locus_tag="ACG89C_001355"
     CDS             complement(8417..9391)
                     /locus_tag="ACG89C_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4899901.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9691..10563
                     /locus_tag="ACG89C_001356"
     CDS             9691..10563
                     /locus_tag="ACG89C_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899902.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11145..13343)
                     /locus_tag="ACG89C_001357"
     CDS             complement(11145..13343)
                     /locus_tag="ACG89C_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899903.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13575..14567
                     /locus_tag="ACG89C_001358"
     CDS             13575..14567
                     /locus_tag="ACG89C_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4899904.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14627..16048
                     /locus_tag="ACG89C_001359"
     CDS             14627..16048
                     /locus_tag="ACG89C_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4899905.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16041..17540
                     /locus_tag="ACG89C_001360"
     CDS             16041..17540
                     /locus_tag="ACG89C_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS4899906.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17579..18973
                     /gene="uxaC"
                     /locus_tag="ACG89C_001361"
     CDS             17579..18973
                     /gene="uxaC"
                     /locus_tag="ACG89C_001361"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4899907.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5733 a         3883 c         3366 g         6080 t
ORIGIN      
        1 taacaatata tacaaacaaa aaaattcact aactagtgaa aataagttag tgaacctcat
       61 ttattaaaga attaaattac agatgatctt ttccatcata cttgaaaaac tcaaaatcag
      121 cataacttcc tcttttttta tttagatcat gcactgctat cccgataaaa tttccagtaa
      181 accctcctga gagtatacac atgttttttt gcggacctac tttcttccat tcttcagttg
      241 atacgtcttt caaatgaaac tgtgctgtct ctttctttac agtgactttc aaaaaagtct
      301 tcttttctgt gagtggaatc gtccaatctt ctatttgtgc tactccgttt tctgattcta
      361 tcatacgtag gcattttccc ttgtcctcat tccaagtaac ataacaataa atataattgg
      421 attcatttaa gtacagtacc agtccagcca tctgattaaa attattaggc atgtattcaa
      481 atgctgtctc cgcatcaaaa gtaaaatccg tttgccgaat agctaacaaa tgatgttcga
      541 aggtagattg aagagattca ccggaaatca gacgtaaaaa acctggtcta cattttgtat
      601 cacaccaatc agcatttggc aaaatgcgtc tggcattcca gttcttcttt agctctcctt
      661 caaatgtatc cataaactga tgatcttttt cttgttgtac ctcttcattt gtctcaatca
      721 ttatttcttc catcggagtt gtctggctat ctttaagtct taaccaatcg tcggacgtcc
      781 aatacacttc ttgaatagcg gtttctcttc ccaaaatagc cgcaccgttt aatggtctgg
      841 tacaaagata tgtcatgtac catcttcctg acggggtttg tactaagctc ccatgtcctg
      901 tacattgcaa tgtagaagca ggtttgtcac tagctgtcag cataggaaaa ccaggatcaa
      961 gctcgtaagg accagtaata ttctttgctc ggcaaactgt aactgaatgt cctcttcctg
     1021 taccgccttc tgccgtcatc aaataataat acccgttacg aaaatacaaa tgtggtgcct
     1081 ccgttttcgc taattctgta cctgcaaata ttttatagat gggaccatca agcttcttct
     1141 tctctgcatc atattcttgt aatacgatac cgctggattt attcggtgtg tccagacgat
     1201 aatcccatag ttcattgaca agccattttt ttcccgtttc aagatcatgg aacaaagaag
     1261 gatcaaagcc gttactattt aaataaattg gttcggacca aggacctgtg atctcagatg
     1321 cagtgatcaa ataattgtgg acatctttaa acggacggcc tgcacttttg acatctgtgt
     1381 aaacaagata aaacaatcca tcagcataac tgatctgtgg cgcccatata cttccatttg
     1441 taggatttcc ttgcaaagat acttgagtgg tcaaaatatc cgttgtatgt gaccagttga
     1501 ctaaattttt cgactcatat actcggattc ctggaagcca ttcaaaagag gagacaataa
     1561 tgtaataccg ctctttcact cttaatatat taggatctgg attgaatcct cttaaaatag
     1621 gatttttcac tttgatagtc tgcatgattc aggctccttt tctcattctt actcgatata
     1681 aactgaatgc cccagctgct aaaaaagcaa gtgaaataaa caacacaacg gcaaacagac
     1741 cagtcatgtc ctgtaataat ccaaatgtat tttctaaaga caaagttgaa tgatctacag
     1801 caagcgactg ataacttttt ttaaactctg acgcagacat attacttaaa gtaactgcta
     1861 acccgccaaa gaaaatcaaa gatagactgc caccgaccat tcctagcaat agttcccatt
     1921 ttcttttcat ttctgaaccc cctgcttttc taaatgtatc cattcaggtc ttttgatcga
     1981 tgtttcataa ttgttattac gttcagagaa tttgacagtc tgttcatttt tcttgtcatc
     2041 ccaattatcc caaccagttg ggtgaacatg cccacctatc ttacacgctt caaataagac
     2101 atttgcatac ggacgccacg gtcttcctaa atagacgttt ctagtatcag gttctgcagt
     2161 caaggtacag ctttcaaaac gataccctgt tttttgattt ttcggcgtag atgctgctgt
     2221 gatgtatcca ggatttttac tggctaagct atggatatga cactgatgaa aaacggcatc
     2281 tgccccacca aatatgaaat ccaccgttcc ttcaatccag caatattcat aggtctgacg
     2341 acaatactca ggattggcag aagccggtgt acaaaaagga gtaccatctt tttgcaaatc
     2401 aggcaacgga cccgtgcata aggtatcctg ataaccagat agtcgacagt tttttaaaga
     2461 tacgcgatct cctgaattga ataaagcaac agcctgtcct accttttctc ctggacctgc
     2521 tgtattttcg atgtgaagat tttctaatac gacattcgat ccttctaaga aaaaagtagc
     2581 acttcggaaa gtccctaact ggctaccgtc aagatactcc tgttttgcaa aacgattacc
     2641 ggagataact actgttcctg ctcccacaag tttaaaattg gataatctgc tttctatata
     2701 ttcatcatat cttccttctt ttactatcaa gacctttgga tggctatccg gcaggcctgc
     2761 caaataatcg attccttctt ggatagtttc aaaatccgtc ggggtatcta tagcattctt
     2821 tccaattatc agtttgtctt tcatcctcat aaatgctccc ttatgaattg tttccaaata
     2881 tttctcattt cttgtgtttc catatgaccg cctgttaaag aaaaagaaga aaagttttct
     2941 ggactaccca ttgattgata ctttttgtcc agcccttttc tcagcttcag tacgccttcg
     3001 tccaagcaca tcgtatcgtt ttttccaact aagctaagac gagggcgcgg agcgattttt
     3061 tcttggattg ctaacgttgt ataagctgtc aatagatttg gcacatagta ataaaaccca
     3121 tgatgatcta ggcggcgatg tagcaaaagt gtttcgatgt ccacctgtgc cgctagatca
     3181 atacaaactt tgacacgaga atccaatgct gtcacccacc agcttaataa gcctcccata
     3241 gaaagaccaa tcgttgcgag ccgttgagta tctgtgtctt cccttgtctc caaataatcg
     3301 atcaagctga tcacatcata aatacgcatt ccccataacg tttttcctgt taataaaaat
     3361 tgtttgaaca actcgctttc agaaatacct ccgcgttctt caaatcccca agcatcaatc
     3421 gaccaaacag catatcccat tcctgtcagt acttctgcaa aagatggaga agctaagtaa
     3481 ggagcaccat ggatcaattc acttttcccg actgaaaaat ctccgccgtg agaatgcagg
     3541 taaaggaccg tcggaaacgg tccttctttt tctggataag catagagggc tggtactgtt
     3601 tccaattcat ttaagtacaa ttgatagtat tcaagcataa aaccgttttg tttttctcgg
     3661 tataatcgtt ttccactagg ggtatgtctg ctagatggag cacctaaaaa atcattcaac
     3721 tgcataagca aagtttttac gaacaaccag ttcatccata ttttctgatt gatttgattg
     3781 gaatttctca atactgattt ctttagtatt ttctaataga aatgctggtc cctcgtgatt
     3841 ttcaatcgac atctggttaa aggaaacctt acccgcaaag ccaatataaa atccttgtct
     3901 gctcatttcc tctattcctg tcatcatagc aggaacaccg ggaactgcat cttgggccat
     3961 cgagacatcg atattgtcaa atgtgatttc ttgtatgaat tgttccgcta atccataaat
     4021 aaaacctgca gaagcatgga cattccttgc cgtgatattc gaaaaatgga tccttctgaa
     4081 tgctgatgtc cgttcatcta tcggataagc ctttttttcc catacatacg gttctttccc
     4141 tcgcggacca cagaaataat aaagattcag tataaatgga cacatcacat tatccatgac
     4201 tatattggat actcgaatat cttcaacgat cccgcctcga cctcgtctag atttcaatcg
     4261 tattcctcga tcagtttctt ggaagataca atttgaaatg gtaatattac gaatactacc
     4321 actcatttca cttcctaata cgacaccgcc atgtccatgg accatcgtgc aatttgtgat
     4381 cgtgatattt tcgcaagcga tcctttcgta cgtatcttca gtccctgcct tgattgcaat
     4441 acagtcatct cccacgtcaa tatggcaatt actaatacgc acatttttgc atgattctgg
     4501 atcaattccg tctgtatttg gtgaatccgc tgggttcaag atcgtcaagt tatcaaacgt
     4561 tgcattactg caaagaatcg ggttgatcgt ccaacttggt gactggatca atttaatatc
     4621 tttcacagta attcgatgac agttatggaa actcatcaat ttaggacgag gataagcgag
     4681 attatctggt tcatttctaa aagtatgcca ccattttttt ccatttccat ctaatgtacc
     4741 gaaaccagtg acagagatgt tctctacatt ttgagcatag atacaggaag cataaacttt
     4801 tcgatggact ccttcccaac gtgaatgaac aacaggataa tcttttggat cgtcagagaa
     4861 tttcagaacc gctcctgcgc ttaaatgaag ttcgacattg ctttttaaaa ataaagctcc
     4921 tgttagaaat tcacctgctg gaacaactac ggtcccgcct ccgtcacttg ctgcagcatc
     4981 aatggcttgt tgtattgctt cagtattcaa ttcatcaatt gatgcgccaa atgtcaaaat
     5041 atcatacata tttataatgc ctccacttca atcaatgctt gcaagaatgc accaacgcct
     5101 ttttgatcat tacaaataat tggttcactg atgtagtaag catatgaacc atctcgctga
     5161 tctgctccgc ctaaccctgc tacttgacag tttttattca agttcagcca gccttctttt
     5221 gtttccagaa caaattcatc aagtaatcct tgatgtgatt tcttcacttg tggaagccat
     5281 tcttctttgt tcagcagtcc caaacgtatt cctttagcca ttgcgtaaac aaacatacta
     5341 ctgccggaag cttccagata atttcctttc cgttttgttt ctgttgttac ttgataccat
     5401 gtcccgctgt tttcttcttg atatggaagt aatgcagcca gtgtcttttc tagaatttct
     5461 ttcagcaaag aacaatctgt ttcttcatga agtaattcat aggtatctgc tgcagccatc
     5521 aaataccagc caatcgatcg gctccaaaaa tgatgggata gacctgtttt tggatcagcc
     5581 catggttgga ccttcttttc atcccatgca tgaatcagca aatttgtttt tggatcgaat
     5641 agatgtttat aactgatttc aaactgtctc aaaacatctt ggtaatcttt ttcttcacta
     5701 aaattctgga taaattcagc ataaaaagga gaacccatat ataagccatc caaccaaatt
     5761 tgataaggat aaatttcttt atgccaaaaa gctccttcgg atgtgcgtgg atggtttgcc
     5821 aactgctggg aaaggagttc tgctgctttt ttatattttt cttcgcctgt ttctttgtac
     5881 aataaaaaga aaagctttcc tgtatttaca tggtcgatat tgtattcttc aacgctgtag
     5941 ccacggatcg ttccatcttc ttgcacgaaa tagtcgatat tgtcttttat gaaatcaaaa
     6001 taaatttttt cttgggttcg ttcccaaagt aattggaacc ctttaagtac gacgccatag
     6061 tcataagacc attttccttt ataccacttt tcttcatata agcgaggtgt tctttccatg
     6121 atactttttg ctaatttctt tgaccagttt tccattattt gctctccttt aaaaattaaa
     6181 ataggatttg ggacaaatac caagaagtat ttgtcccaat cgtctatatt actgtgccgc
     6241 tgctttgtat gcttcctcgt actcttgtgt gattcgatcg cctccttgtg ctttccagtc
     6301 ttctacagct tttttgaatc catcttcgtc aagctgtccc atgatgtatt gcaccgtagc
     6361 atcaatgacg attttttcaa tttctgatcc tacttcattg ttcgtcggtg aatccaatgg
     6421 cacagaagga tccattacag caaattcttc attttctttg atcaattgat tagagaattc
     6481 tttttcaggg tctgtatctt tgaaagagaa cgtcacttca ctaggacgag agcttgataa
     6541 cggttgcacc tcttgttgcc aaagatccat atcgataaat tcgatagaac catcatcatt
     6601 caattcataa tgcgttcctt cgataccgcc agtcatcaat ttatacacat cttcttctaa
     6661 aagatcatca acaaattgaa gtaatttctt caattgtgct tcgtctttta cttgagattt
     6721 gggaaatgct agcaagccgc caataccatt tccttctgac caaacatgat aaccgccgcc
     6781 ttcaccatct gagatcttgt ttacaggcac taattccata tcatcttgaa taccttgacc
     6841 catcgtcttc agattcttga tatccaccat tccagtgtag attccagttc ttccttgtgc
     6901 aaattgttct tgctgatcag tcttttgggt aactgcaaag tcttgtgcca aatagccatt
     6961 ctcaaataga tctcttgaaa aattcaaagc tttcatatac tcttctgtat caaattctgg
     7021 agtgaatttg ccattatcgt ctacagccca gccatttggt gctccaaaat aagaagtgaa
     7081 aagtttaaag ctggtataac gaatatcact aggcgcacga tcgccaaaac caactgtgtc
     7141 gtccacacca tttccatcag gatcgttttc tgtaaattgt ttagctactt cataaagctc
     7201 atccatggta gttggaacat ctagtcctag attatccaac cagtcttttc gaatgactaa
     7261 gccagcacga gcgtagtctt tttgtatcgg tacgccgtac aaggcaccgt cgatactcgc
     7321 agcttcgatt ctatcttcag aaatttgact taaattttta tagtctttta aatattctga
     7381 tacatcccag aatacgcctg attttaacgc attgcggaca gaagagtttt tcaacatcgc
     7441 aatttgaagt gtaacgatat ctcctagttc atcagaagcc aatgcagttg ttaatctttc
     7501 ttcttttgaa gcatcaggaa tccagttgaa tgtcagttcc gtatcggttt tttcttcgat
     7561 caagttcaca atcgtgtctg taggcggtga agcatgatgc aaaatattta accaagtgat
     7621 tttagctttt ccatcttctg tctctgaaga actgttactt gaactgctgc cgcatgcggc
     7681 aagtgctaga attcctgcaa aactaagacc taattttacg agcgttgctt ttttcataaa
     7741 ttttctcctt cactgtttta ataatatttt tgttttcttc tgttttttct gtatatgagt
     7801 tcttccattc caaatggcaa aatatagaat ttgctgtcca cgtgactgcg gaaaagaaga
     7861 gactgccacc gataaaggtt aaaacacctg gcattgtttg gatgatcatc agataaatcc
     7921 ctaccatact taatatcaac actaatgagt aatgaagatg tgagaatccg aataaaaatg
     7981 ccattttcat tttgaaaaaa tatcctttcc aatcataatg agccatgatt gggaaaatat
     8041 agagtgctga gagcacatat atgaacgcag caatatacaa agcgatccta agataccaat
     8101 tgtgtatgaa agaaagatcc acaagtaaaa caatcccgat ggcgccatat atccagctca
     8161 tcagcaaggt ttcacgatag ttttctttaa aatatgtgaa gtacgttttc gtcaaaggaa
     8221 acgtttcttt tgagcgcagc cattttctaa tcactgcaac catcgcatac gatgcaggac
     8281 cgaaagtaaa aactcccaaa cctgcgatga ctaaaccgcc ccacagaaaa ttcaaataaa
     8341 ccaactgtaa aatacgattt agccttccgt ttattttatc taatgactgc agcatatctt
     8401 tccctctctt tctatcttag taaacgccgt cttctcctag ctttttggca atcttattag
     8461 ctaaaattac cataaacaat cctacgaccc ctttaaacaa tcccattgct gtactgaaac
     8521 ttaactgtcc attcttcaat ccagctgtat aaatataggt gtcgaaaatt tctgcaacgc
     8581 ttcgattcaa tgaattcaat aacaagtaca tatgctcgaa ccctaagtct aatgtctgac
     8641 cgatttttag aatcagcaag gtaatgatta ctggacgaat tgccggcaat gtaacatgcc
     8701 atgttttgcg taagcgtcct gctccatcca tttctgcagc ttcatacaac tgtgtatcga
     8761 ctgcagttat cgctgaaagg tagatgatcg tagaccagcc caactctttc cagatcactt
     8821 gtccgatata tactgtgcgt gtccattcag gggatgttag aaaactgatt ttttcaaatc
     8881 ccattgttgc aaggaattca ttcaataccc ctccgtcaac attcaaaaat acataagtaa
     8941 tcgaaacaat gattacccaa gacatgaagt gagggatgta aatgatcgtt tgaatcccgt
     9001 ttttcaatcg tttgttcgtt acttcattta atagtaaaga caaaatgatt ggcatcggaa
     9061 aaaacacgaa gatatttaat ccgaacaaga tcaacgtatt tcttaataat gtaaagaatg
     9121 tcggttctgt aaacaaacga atgaaatgtt tgaaaccgac ccaaggactt cccattattc
     9181 ctaaaaatgg ttgataatct tgaaaagcaa taacgagtcc ccccatagga agatacctaa
     9241 aaatcaagaa ataaaggact cccggcagaa tcatcacata taaaaattta ttcgttttga
     9301 ttcctgcaat tatttttttt cgtcttctga gcttttcttc ttttttcgat agcgtcagtt
     9361 gaaatggttc ttgtttcttt acactttcca taagcccctc cttagttttg tgaatgtttg
     9421 ctgcttatga cttcatgata gataactatc tgaattacgt atataatcat ttttatcgta
     9481 agtaaaaacg cttacatagc aatgattttt gtgctataca aaacgataaa gtgtattttt
     9541 tcacaaataa actacgcata gcaaaccttc tatttttttc tatctgacac ttagcatagg
     9601 aaatgattat atacgtaatc tttcaccgat gataaattga acatatagaa acagaaacat
     9661 ttaatttatt ttgaggagga gcctatctat atgaaaaaga aagtctctat aggagaaaga
     9721 agttttacac ttttcaacaa tacgtttcta attttgctag ctttgatctg tatcgttcct
     9781 tttttaaata tcatcgctac atcttttgct tctacacaag aagtcgtcgc gaaaaagttt
     9841 atcctttttc ctactacctt ttctttagac gcgtatcgct acattctttc tacgccgacc
     9901 atttttagag cacttgctgt ttcgattggt gttacaggtg tcggaacgat cgtcagtatg
     9961 tgtgcaactt cactgatggc ctacggtctt tccagaaagt atttgtttgg tcgaggtttc
    10021 gtcaactttc ttgttgtatt ttctatgttg ttcagcgggg ggatgattcc aacgttctta
    10081 gttgttcgtt ctctagggct ggtcaattct tactgggcca tgatcctgcc tgtggctgtt
    10141 aatgcaatga atatgatcat tatgagaaac tttttccaag ccttgccgga tagtttggaa
    10201 gaatcagcaa aaatggatgg ttgcacagat tttggtgtat ttttcaaaat tatgctgcca
    10261 ttagctttac cgtctattgc tactatctcg cttttttatg ctgttactta ctggaacact
    10321 tatatgactg ctattttgta tatcaacgat tcatctaaat ggccgataca gattctttta
    10381 cgccaaatcg tcattgtatc aagtgggatg caagcagaaa gtagtgccgt tgatgtgatt
    10441 ccaccagcac aaacagttaa aatggctgtt atcgttattg caacagtgcc tatgctgatt
    10501 gtttatcctt ttgttcagaa atattttgtc aaaggagcac tcgtaggttc tgtcaaaggg
    10561 tgattatgta ctgaacgcaa aatggtagtt gtgtcagaag tggtcagctt caagaaataa
    10621 gaaggcattt acgaaaattg cttcttaaat ttttgtgaaa tttcggctta tttttcgaag
    10681 aatctactcc tggaacaccg tgtatcagag gttgtgaaag agacgttcag ccttgagcga
    10741 tgataaaaac tctcacaaac attgcgacga gtaacccaca ggagcaataa gaagcgattt
    10801 tgaaaaatag cttcccatat ttcacaaaat tatgccttat tgtcgatagc ctgtctcttg
    10861 aacgccattt attcgtaaaa aagaactgtg acaagacttt tgtcacagtt ctttttgcgt
    10921 gcttagcctt gaaatttaac tatccataaa tacaaatact agggttattc taacttcttt
    10981 tattgcttga atcagtccac aagaaaaaca gcctcttgat taaacggcgt ttatcttgtt
    11041 gattctgcgg caataaaatc agccagcagc aaaacatgaa gtgctgtttt ttgctgtcct
    11101 cttttattgc ttgaatcaga tcacaagaaa aacagcctct tgattcacaa atattttttt
    11161 cgatattctc ccggcgtaat cgattccttc tttttaaaga aacgaataaa attttgcgga
    11221 tttcgatatt gcaatctttc tgcaatctct tttacggaga ggttagtctc tttcaaccat
    11281 ttttttgcta cctctaaacg atgattttga acaaaatcac caaatttttc tcctgtttcc
    11341 tttttaaata cattacttaa atagttaggg ttataatgca acctatcagc gatcacatct
    11401 aaagacagat cacgatcata ttctgtgtag acgatatgca taatattctc cgacaaattt
    11461 ttaaattcat gatcagtgat tgcttgagag cattctgtga ttggcagaac taattgttct
    11521 aaaacaagtt tttctagttc tttgggatga taattgttca gtgcttttac ataaatttgt
    11581 ttcatgttat caaagttttt tgaatctact cccaataact gtcctaattg aacgagttca
    11641 ttgatcaagc ggaccgaaac tacttcccga ctcaaaggat ttttattttg tgcaaacaat
    11701 gcatcaatca attcatgtac taaattcttc acttcctgat ttccagaacg aatggcttca
    11761 aacaatcgat tttgttcttc tatcggatat ctaataagtg ttttttcatg agcagcagga
    11821 acgatttcct gataaaaaat aattgaactt ggaccagtat ttacacgata atacagggct
    11881 tcttttgccc gatcaaccga ttgcttgctt tcattcagcg tttcgaaccg gttactgatg
    11941 cctatactga tagttaaatt caagtattct tttacacgtt tttgaatgag tcggcaatac
    12001 tccatgactt ttttgcttgc ttctgcatca tctttatcca ttcgataaat agtggcttgc
    12061 atttcttcat tcaaaacgat cggcagcatt cgattttcct ttggcacaat ttccgtgatc
    12121 atgttgttga tcgctaaaag tagcaaatca cgctccccac cgtgactatc ttctaacaga
    12181 tcgatttgaa ccaaagctgt ataatagcag tcattttgag aactataacc aaattgctgc
    12241 aaccgctgat ctagttctcg cctttctact cgatttctaa ataaactaag tacaaatagc
    12301 gtttccagct gtggcttttg ggtaactaag tgtgcactta acgcttcttt ttctcctatg
    12361 atccgactga ctgattgagc gaccaaatct agttctttcc cactgaatcc ttttgattta
    12421 agattcagac gttcctgtat ttgagaaatc ggacgggcaa atctgtccga aaataagtag
    12481 gaaaaaagac caatgagtcc taccagtgtt aaagacactg caactagccc gattctcaga
    12541 ttgcggatca ttgcgccaat tgcactagag tcaacttcta atctgtaaat ccaattatta
    12601 taatctgatt tcagtactat atacgtttta tctttttctc ttatcacttt cagctgattg
    12661 taatctagat ccacgtccgt taatacagtt ggttgctctt tattttttac tgtctgagta
    12721 gtaaaaaggt tgccaacttc gttttcaata gaaagtatgt cttctccact ttcaccaaca
    12781 acttgttcta ttgtatgttt ctctatcgta gcaattccca atgcatattt ttctttttca
    12841 aacattggca aagaaatcac catttcgatt cctttgtttt tcttttgcca aaataaatta
    12901 tttttgttat ttatgtactt ctgttgatac ttttcaactt cctcttcggt cagttgactc
    12961 aacgatccat tgattactcc ccatttacca gccaaactga ctaaatcata attattatca
    13021 tccataacaa tcgtttctat aaaatttagt tctttcctga tttcagaata agtggagtaa
    13081 tctgtatatg tcagcggatg cttgaatatg tccgagaaat ttttggtcga accatacgta
    13141 gaaaacgaat attcaatcgt tctaattttc cattcaacgt tagttttaac ttgcctgagc
    13201 aaattctttt tgtctgttaa ataactcgat tcgatcgatt cataggtatt ctggtaaatg
    13261 aataaactaa atcctatgac aagtacggct cctaataaaa acatcggtaa aaatatttta
    13321 taaaatactg tacttttctt cattttcttc ctctcctcct tttaaaataa aaggctttca
    13381 tttttctgta aaattgatac tcatctaatt aaatgagcat tccccgcttt caagaatagc
    13441 ataattaaaa ataaaaatcc aagtaatttt taacgtgtgc aatcttttca tatcaaagat
    13501 ctattgactt atcgtagtta tcgtaatatg atgaatgcgt taacaaaaag tgaaaaagta
    13561 aggaaaatga tggaatggta acgatcaaag atatcgctaa agcagctggc gtttctcata
    13621 cgacagtttc tcgtgctttg aataacagca gtttaataaa aaatgaaaca aaacaaaaaa
    13681 tacgacaact agctaaggaa atgaattatt cgccaaacta tagtgcaaaa ggtttagtga
    13741 atcaaaaaaa atatctaatc ggactatttt tctcaagttt gcataaagga acttcatcaa
    13801 atttcctagc agattccatt gcaggcatcc attcagtatt agacacaaat tacagtttat
    13861 ctgttgaaag tatcgatgtt gttgatttaa cagaaatcaa cctgcaacgc tacgatggac
    13921 tgatcgttat gagtcaaagt gatgatgatc agcctttcat cgactatctc aaagaaaaag
    13981 aatttccttt cgttgttgtc aatcgtcaca tcaatgatcc agcggttatc aacgtcactg
    14041 ccgatgatgc ggccggtgtt atgcaggcag tagattacgg tattggatgc ggacatacga
    14101 agatcgctta tattggcgga aagaaaaatt ttcgttcgac aaaagaacgg gaaaaagggg
    14161 tattgaaaag tctaaaaaaa gcgagcatcc ctgtcaatct agcctatttt ttaacaggag
    14221 attacagctt agaaagcggc ctccaaaaca tggaatacct attatcactt tctgacctgc
    14281 ctactctggt tttttgcggc aacgacgaca tagcgattgg tgcattgaga gctgtacatt
    14341 tagctggcct ccgtgttccc cagaacattt ctctgatcgg gttcgatgat tctccagtcg
    14401 tctcctatct caatccgccg cttaccaccg tacgtaagcc tttgaaagaa atgtgtcaag
    14461 aaggaacaaa gcttttacta gatttgatcg aaggcaggac gatcgaacaa tctaaaattg
    14521 agctctccac cttgttgatc cttcgtgagt cgatcaatta cttgtaaact acaagtaatt
    14581 ttatataaaa atgttaacgt gtgcataaca cagaaaggaa gtttatatga aaaaactgac
    14641 aaaagaatta gtgaaaaaaa acgaagcacc tgtaaaagtt cttcagtttg gtgaaggcaa
    14701 ttttatgcgt ggatttattg attggcagat tcagcagctg aataaccaac aattgtttaa
    14761 aggtaatgtt gcaatcattc aacctttaga acaaggtctt ggaaatatga tgaaccagca
    14821 agataattta tatacggtca ttttacaagg actcagcaat ggcgaaatcg ttgatacttc
    14881 cgagattatc accgtagtag aacaaatgat caatccttat gatgaatggg atgcttatct
    14941 atctctagca gaaaatgacg acttggccta tatcgtctcc aatacaacag aggcaggaat
    15001 ccaattcgtg gagactgata atttaacaga tactccccca cagagcttcc cgggcaaact
    15061 gacagccttt ttataccgac gtttcaaatt aaacaaagct ggctttacca tcattccttg
    15121 cgaactgatt gacagaaacg gcgaacgtct aaaagaaatc attttacgct acgcagaaaa
    15181 atgggattta gaacctgctt ttgttgagtg gttagaaaaa gaaaatgttt tctgttgtag
    15241 tttagttgat cgcatcgtcc caggttatcc tcgcgaagat gcaggtgttt tcaatcaacg
    15301 gcataactat gaagatcaat tgatggtcaa agcagaacca ttcatgcttt gggtgatcga
    15361 agggcctaaa gaattggaga atcaactacc attcaaacag gctggcttga atgtcatcat
    15421 cacagatgat atgactcctt atcgtgaacg taaggtgcat ttgcttaatg gaccgcatac
    15481 agcgatggtc cctcttgctt tgttggcgga attggaaact gtagaagaag taatgaaaga
    15541 tcctttattc tccgcatatg ttgatcagtt attcaatcta gaattgattc ctatgctttc
    15601 tttacctaaa gatgaattag ccatttatgc tgaccaaatc aaagaacgtt tcttgaatcc
    15661 ttttgcccat cacaaattag aagcgatttc attaaatagt gtttcaaagt tcagcacccg
    15721 tctgcttcca gtgttcaaga agtatatcga agaacaaaat caggtaccac cgttaatcac
    15781 tgtttcacta gctgcgctac ttcttatgta tcgaggcgat caagtaaagc cacatgatga
    15841 tgaaaagacg ataagcgaat ttactgatgc ttggtctgat aatggaacag ctattcctcg
    15901 attattgcaa aacgctgcgc tttggggaga agatttgtct caaataccga atgtgacaga
    15961 tacagttcaa gaaatagctg caaagatcga tcaaaaaggt gtccggtcag tattacaaga
    16021 aatgaaagga acgatggtcc atgcttgaaa ccctgcaacc aacaatgaaa ttaaatcgac
    16081 gagacagtgt ggtcgtcgca ttaaccccta ttccaaaaca aacacaactg acgattgatc
    16141 agcaaacgat caccactctt gaagatatcc cgcaaggtca taagattgca ttgggtgatt
    16201 tgaaagctgg agacaacgtg atcaaatatg gctatccaat cggtcatgtg acgacagacg
    16261 taactgccgg tcaatggctg cacactcata atgtaaaaac caatctttct ggtgaattgg
    16321 attatcgata tgaaaaagat gtccatccaa gtcattacct ttttgagaac cgaacatttc
    16381 aaggttactt gcgaaaaaat ggaaaagtag ggattcgtaa tgatttgttt ttagtaccta
    16441 ccgttgggtg tgtgaatggc attgctgaac tgatcgtcaa acagttcaag gaaaaacatc
    16501 cagatctggg acagtttgac catattacga ttttaaagca tccttatggc tgttcgcagc
    16561 ttggaaaaga tcatcaaaat actcgagaaa ttttagcgga cgcagtaaat catcccaatg
    16621 ccggaggagt tttagtattt ggattaggtt gtgagaacaa tacagttcct gaattcaaaa
    16681 aaattttagg tgaatatgat gaagaacgag tgaaatttct agtggctcaa gatgtttacg
    16741 atgagatcga acaaggtgtg gctctattag aagagctttt aacagcggcg gaaaatgacc
    16801 agcgcatatc agtcccgctc tctaagttga atgtcggttt gaaatgcggc ggttctgatg
    16861 gtctatcggg aataactgct aatcctttac ttggtgcatt ttctgactac ttgattgctc
    16921 aaggaggcag cacgatctta acagaagtgc cagaaatgtt tggcgcagaa caagtattga
    16981 tggcacgagc ggaaaatgaa gaagtttttg actcaatcgt tgacttgatc aatgacttca
    17041 aacagtattt cttgtcttac ggggaaccag tttacgaaaa cccatctcct ggtaataaag
    17101 caggtgggat cactacatta gaagataagt ctttaggctg cacgcaaaaa tcaggaagct
    17161 ctcctgtcgt tgatgtgcta caatatggcg aaaaaatcag aaaaccaggg cttagtttat
    17221 tgcaagctcc cgggaatgat cttgtagctg cctctgctct tgcttcatcc gattgccagt
    17281 tggtcttgtt taccacagga agagggactc cgtttggctc atatgtccca acggtcaagg
    17341 tatctacgaa tacaactctt tttgaacgca aaggtcattg gatggatttt aatgcaggcg
    17401 tcttattaga acaaccaatg gaagtaatct tagaatcgtt tgttcaaaaa atcatcgctg
    17461 tagctagtgg agaagaaacg aaaaatgaac aaaatgacgt tcgagaaatt gccattttta
    17521 aaaacggagt aacactttga ttttcataaa acagtataaa aagaaagaag gaagatttat
    17581 gtttttatct gatgattttt tattaaaaga cgattgggcg aaaaagcttt accattccta
    17641 cgcaaaaaac atgcccatta ttgattatca ctgtcactta gatccaaaag aaatttacga
    17701 aaacaacaat ttcagcaatc tgacagaagc ttggttagga ggcgatcatt acaaatggcg
    17761 cttgatgcga gcttgcggcg tgcctgaaga aaaaatcaca ggaaatgctt ctgattttga
    17821 aaaattttta gcttggtgtc aaaccgtacc aaaaattatc ggaaatccgc tttacagttg
    17881 gtcacatctt gaattgaagc gtttcttcgg tattgacttg ttgataaatg aagaaaatgc
    17941 agagaaaatt tggacacaag caaatgaaat tttggcagct cctgattttc gccgtcggga
    18001 aatcgcaaaa aaatcgaatg tagaagtgat ttgtacaaca gatgatcccg cagacgactt
    18061 gcactatcat cagttgcttg ctcatgaaga accagatttg aaggttctac cttcattccg
    18121 tccagataaa gcactcaata ttgaaaaaga aggatttaat gcatgggtca cactactgga
    18181 agaagcagct gaccaaacga tcaccaccta tcaagaactg atagaagtct tagggcagcg
    18241 gatagaatac ttccatcaac atggatgtcg tgtttccgac cacgctttag acatcttacg
    18301 ctataaagaa gcggatgaaa cagtattaga agagattttt caaaaagcac gagctaacaa
    18361 actattatct tcagatgaaa tcgatgctta tcttacagaa acactgatac atttgaccca
    18421 cttctatcat tctcataatt ggacgatgca gctgcacatc catgcttatc gtaactgtaa
    18481 tacacaaatg ttggaaaaat tagggcccga taccggctat gatggaatga atgatctttc
    18541 tttgactatt cctctgcaaa aattattgaa ccgtgcagaa gaaacggatc agttgccaaa
    18601 gacgatcctt tattcattga atccgaatga ctatcctgcg attttagctt tgatgggctg
    18661 tttccaaaaa gaacagaatg gaaaactgca gttaggttct ggatggtggt acaacgatac
    18721 tcgagctggt atgcgcgatc aattgacaca atttgctgat ggtagtgctt tggggaattt
    18781 tgtcggcatg ctgacagatt caagaagttt cctgtcctac actagacatg aatatttccg
    18841 tcgtgttctt tgtgaatttt taggcgaaat ggtcatgcga ggagaagcac ctgaagatga
    18901 acaactttta ggaagtctag ttcaagcgat tagttataca aatgctaaaa attactttgg
    18961 cttttttgcc taattgatta atagtaacag taacgaatca aaaacaggat agtgaaataa
    19021 cggaatccgt ttatccacgt tttagagaag aagacggacc aa
//
LOCUS       DAZUKR010000043        18863 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000043 DAZUKR010000000
VERSION     DAZUKR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.043"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACG89C_001362"
     CDS             complement(17..538)
                     /locus_tag="ACG89C_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS4899908.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="ACG89C_001363"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="ACG89C_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4899909.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /gene="cdaA"
                     /locus_tag="ACG89C_001364"
     CDS             4618..5505
                     /gene="cdaA"
                     /locus_tag="ACG89C_001364"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS4899910.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="ACG89C_001365"
     CDS             5502..6671
                     /locus_tag="ACG89C_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS4899911.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /gene="glmM"
                     /locus_tag="ACG89C_001366"
     CDS             6695..8050
                     /gene="glmM"
                     /locus_tag="ACG89C_001366"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4899912.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACG89C_001367"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACG89C_001367"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4899913.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="ACG89C_001368"
     CDS             10336..10626
                     /locus_tag="ACG89C_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899914.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="ACG89C_001369"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="ACG89C_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4899915.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="ACG89C_001370"
     CDS             11667..12326
                     /locus_tag="ACG89C_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4899916.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="ACG89C_001371"
     CDS             12483..13415
                     /locus_tag="ACG89C_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899917.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /gene="tig"
                     /locus_tag="ACG89C_001372"
     CDS             13569..14858
                     /gene="tig"
                     /locus_tag="ACG89C_001372"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4899918.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="ACG89C_001373"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="ACG89C_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS4899919.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /gene="yihA"
                     /locus_tag="ACG89C_001374"
     CDS             16438..17025
                     /gene="yihA"
                     /locus_tag="ACG89C_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4899920.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17099..17254)
                     /locus_tag="ACG89C_001375"
     CDS             complement(17099..17254)
                     /locus_tag="ACG89C_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS4899921.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17390..18718
                     /locus_tag="ACG89C_001376"
     CDS             17390..18718
                     /locus_tag="ACG89C_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899922.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         6162 a         3362 c         4067 g         5272 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtatttaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg agcttaactt
    17101 acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga aatcgatcga
    17161 tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg aaaagtttat
    17221 ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg tttcttttta
    17281 cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt agtttttctt
    17341 tatcattggt tataattaaa ttataaaaag atttgaaaga ggtgagaaaa tgattggcga
    17401 agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa aaaaacagga
    17461 aataagcgat tgggctattt tgacacgtct gttgatccag ccaacattta tccgctcttt
    17521 cacacaacaa gcggatccgt atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc
    17581 ggtaagagac gaaagttggt tagttgtagg aaatgacgag aacgaatgta gttttcgctt
    17641 ggaagataat caattattga taaaaaatgt attgtctatc tctgttttca aagaaaagca
    17701 atttttgatt cgagacttca ttcagaaaaa aatgattgct catggggtgt tcgcctatat
    17761 gcgagcttat agcgagttta tctatcacaa tacaaaacag atctctcaac ggctattgtt
    17821 tgagaaaaag gatgaaatcg aacatttacc taaaatgaaa caacaaaacg gggaagtagt
    17881 agtcgattgc aaccaatttc caggatacga tcttttttac caaggtttat gtttcacttc
    17941 gtgttgggaa atgtattata gtcgctatta tcatcaaatc atccctaaac cgatttttct
    18001 cgacatccaa caagtagaaa aagtgaagga attagaaaac gaagtcatat gtatccagct
    18061 ttatcgagac ccctttaact ggcaaaagcc aaacaatcaa atgtttcaat cctattttag
    18121 ggaccagctt gggtttgatc atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc
    18181 attcgttgaa tacatttaca ctgaccatac gatccaatct gtccagtatc agaatgcaca
    18241 aatgcaacct gttcctaaaa agaatgcttc attttttgta acaaaaagtt atgatatcca
    18301 acaaggagat tataaagaaa ggcgtgttcg cggaacacta aatgctcagg cgtattttcc
    18361 ttgggttgac gagaatcgat cacgcatgat gtgctataaa gttatcgatc cgactgttgc
    18421 tttggataat ggaatcgaag cttattgtta ttatattagg gaatatctag aagtagaggt
    18481 aacagatgaa aaatatcaag agtatctttt atccttaaga atctatgttc cttcagaatc
    18541 cctgtcagaa ttaccgttaa aggaaatcaa acatagactc tctgatgtta ccttcaaacg
    18601 aataaagaaa aagcggcgaa gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg
    18661 tgtgcaattt ttagattacc gagaattaga acaattgatt actttacaaa aaatatgagg
    18721 tgaagaaaat ggataaacaa ctctattatc gaataatggt tccggcatca ttattatccg
    18781 tctgtctctt ttttgttcct aattttccta ttcgatcagt catcggtttt ttggtcatgt
    18841 ttggcgggat gggactttat tat
//
LOCUS       DAZUKR010000044        18176 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000044 DAZUKR010000000
VERSION     DAZUKR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.044"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..148
                     /locus_tag="ACG89C_001377"
     CDS             <1..148
                     /locus_tag="ACG89C_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4899923.1"
                     /translation="FAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYM
                     KKTL"
     gene            complement(226..759)
                     /locus_tag="ACG89C_001378"
     CDS             complement(226..759)
                     /locus_tag="ACG89C_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS4899924.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(853..2031)
                     /gene="mutY"
                     /locus_tag="ACG89C_001379"
     CDS             complement(853..2031)
                     /gene="mutY"
                     /locus_tag="ACG89C_001379"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4899925.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2024..2821)
                     /gene="recX"
                     /locus_tag="ACG89C_001380"
     CDS             complement(2024..2821)
                     /gene="recX"
                     /locus_tag="ACG89C_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4899926.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2928..4310
                     /gene="rlmD"
                     /locus_tag="ACG89C_001381"
     CDS             2928..4310
                     /gene="rlmD"
                     /locus_tag="ACG89C_001381"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4899927.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4484..5062)
                     /locus_tag="ACG89C_001382"
     CDS             complement(4484..5062)
                     /locus_tag="ACG89C_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS4899928.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5384..5854
                     /locus_tag="ACG89C_001383"
     CDS             5384..5854
                     /locus_tag="ACG89C_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS4899929.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5962..7188)
                     /locus_tag="ACG89C_001384"
     CDS             complement(5962..7188)
                     /locus_tag="ACG89C_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4899930.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7942..8142)
                     /locus_tag="ACG89C_001385"
     CDS             complement(7942..8142)
                     /locus_tag="ACG89C_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4899931.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8276..8884)
                     /locus_tag="ACG89C_001386"
     CDS             complement(8276..8884)
                     /locus_tag="ACG89C_001386"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS4899932.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8969..9439)
                     /locus_tag="ACG89C_001387"
     CDS             complement(8969..9439)
                     /locus_tag="ACG89C_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS4899933.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9487..10401)
                     /locus_tag="ACG89C_001388"
     CDS             complement(9487..10401)
                     /locus_tag="ACG89C_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4899934.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10547..11350
                     /locus_tag="ACG89C_001389"
     CDS             10547..11350
                     /locus_tag="ACG89C_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS4899935.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11528..12475
                     /locus_tag="ACG89C_001390"
     CDS             11528..12475
                     /locus_tag="ACG89C_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4899936.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12543..12836)
                     /locus_tag="ACG89C_001391"
     CDS             complement(12543..12836)
                     /locus_tag="ACG89C_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS4899937.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12864..13529)
                     /locus_tag="ACG89C_001392"
     CDS             complement(12864..13529)
                     /locus_tag="ACG89C_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS4899938.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13669..14301)
                     /locus_tag="ACG89C_001393"
     CDS             complement(13669..14301)
                     /locus_tag="ACG89C_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4899939.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14308..15375)
                     /gene="liaS"
                     /locus_tag="ACG89C_001394"
     CDS             complement(14308..15375)
                     /gene="liaS"
                     /locus_tag="ACG89C_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS4899940.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15372..16103)
                     /gene="liaF"
                     /locus_tag="ACG89C_001395"
     CDS             complement(15372..16103)
                     /gene="liaF"
                     /locus_tag="ACG89C_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS4899941.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16272..16751)
                     /gene="greA"
                     /locus_tag="ACG89C_001396"
     CDS             complement(16272..16751)
                     /gene="greA"
                     /locus_tag="ACG89C_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4899942.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16887..18062)
                     /gene="mltG"
                     /locus_tag="ACG89C_001397"
     CDS             complement(16887..18062)
                     /gene="mltG"
                     /locus_tag="ACG89C_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS4899943.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5392 a         3686 c         2907 g         6191 t
ORIGIN      
        1 ctttgctaag aatcaaggct ataatacgat tttattaggg gcacagtcca cagctgaaac
       61 tttttacgaa aaactaggat atacagctta cggcgatcct tttgaagatg ctggcatgcc
      121 tcatatttat atgaaaaaga ctttataaaa atttatttcg aaaaaagcaa aaaacagcaa
      181 gcttttgaga aaaatctctc actggcttac tgttcttttt tgtgtttact tacgcgacaa
      241 ttgcttgtaa cggttatacc aaatatcaat atagccttcc gagaacgggc catctccatt
      301 attgatccag tcaaccagaa tcttcacatt ttcttttagg atgaagtcaa tatcgttcgg
      361 gtaattcatc attttgctgt gcatctcata ttcatcgaca tccaatagtc gtttctcgcc
      421 gtcaggaaat accttgatat ccaagtcata gtcgatatat ttcaaggcct catcgtccag
      481 caaaaaaggt gaagctagat tacaataata ggaaaccccc ttttctctga tcattgctat
      541 tacattgaac cagtattttt tatgaaaata aacaatcgct ggttcgcgcg ttacccatcg
      601 gcgtccatca gattcagtga ctaatgtgtg atcattcacg ccgatcatag agtgttcgct
      661 tgtttttaat accatggtat ctcgccacgt tcgatgcaat ttgccgtcgt gcttgtaact
      721 ttggatcgtc acaaactctc cttcttttgg aactcccata gcaacccttc cttaagcagc
      781 attttgtgct ttgtgtttgg gtgttcatta caccgacatc ataattatta tatcaataaa
      841 aatgagcttt gcctagaaag gattcctatt gtttcgattt ttctctaact gatctaccag
      901 tttcatttgg accttaggaa aaacgaccga atcaaatgca tcaggcttca accacttcga
      961 agttttgttc tcggtaaatt cttgttctgc tccctcggtt gcccgtccgt aaaaaaggag
     1021 aacatgccat ttcagatgac taaaaatatg agtaacttct cctaaatgtc gtgtctgcca
     1081 aacaaagagt tcctgctttt caaatgggag attttgctct tcttctgcta ctaggtcatc
     1141 aaacagatcg atttctggct ttgtttccca ttcttttttc aatcgctcat actcctcttg
     1201 tgtcacttcc atcattggaa atgtccacat attcgccagc aatttttggc tatctctttc
     1261 ttcaaaataa tacgctccag aatgattttg aagagctgca ctgatataat aaacgtcttt
     1321 tggctttgct ttttttgttt ttacaggaaa actcgtttgg attcctcgtt tgttagctaa
     1381 acaaaaggct tggatcggac aagtctcaca ctttggagaa gttggtgtac agatagctga
     1441 acctagatcc atcattgctt ggttgaattc acctggatgc ttttcatcaa tgattttccg
     1501 catcgcttca tcaaagattt tcctgctaga agcttttgcg atgtctgctt caatgcaaaa
     1561 caatctgctg actactcgca tcacgttgcc gtcaactgca ggttctggaa gtccaaatgc
     1621 gatacttgca atcgcgcctg tcgtatatgg tcctattccc ttcaatgagc taatttcttc
     1681 aggcgtttgc ggcatttccc catcaaattc tgacatgatc tgtttagcag cagcttggat
     1741 gttccgggct cttgagtaat aaccaaggcc ctcccatgct tttaaaagct tttcttctgg
     1801 agcatttgct agctcttcaa ttgtaggaaa ccattccatg aaacgataaa aataatcgat
     1861 cactgtgtct actctggttt gctgaagcat gatctcagag atccagatac gataaggatc
     1921 acgattatag cgccaaggca ggtttctttt ttcttgttca taccattgaa tgaactgatc
     1981 ttgaaattct tttgtttctt tatctgtcca tttttcccat tctttattca ctttcctctt
     2041 ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa tcctttttga tataagcttt
     2101 gctttacttt tagatttctt tttttaggtt caaaccttaa atttcttcgc catagtttat
     2161 ctccttgttt gactaaataa tcatattcta tttcttgatc tgcttcttgc ggaagatcag
     2221 ctatcgcttg ttggatcact tcttgagaaa agcctttcgt catcaatgtt tgttggattt
     2281 ttctcaataa ttctttttgg ctttttccgt gattttttcc aaacgctttt tcggctgtct
     2341 gatgtgcatt ttgaacttgg tcttcaaatg tatattggct gagcgcttgt tcaatgattt
     2401 caggtttcaa acctttttgc tgcatctgct gcgctaattt ctttggtcct ttgtcactta
     2461 agcgcatatt cgtgcgcaca taactttccc catagaccaa atcatcgaca aggtccaatt
     2521 cttttaagcg ggctacgatt ttatttcgat cttcttgaag gatttccttt tcttttaagt
     2581 atgttcggat ttctttttcc gatcgcaact gataacttaa gtaattcatt gctaggtgaa
     2641 aaccgaaatc agctccgcta ctcttcttga tctcttggat agtttcttcg tctaattccc
     2701 gtccttttaa taatcgatag cgtactaata catcttcaga tacttgtaaa gcttctcctt
     2761 cagataaatc tacgcgatag aaagggcctt tgcctttact gattctcgta acggttaaca
     2821 taaacatcct cctcgaacat atattctcta tcataccaca gaaaatcccc gtaatcaggt
     2881 attatatgtt agaataaacc acagaaaaaa gaaaaaggga gatacttatg gcagaaacaa
     2941 ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg tttaggcata aatggtgaag
     3001 gaatcggtta tttcaaacgg ttgatcatat ttgttccata tgcactacca aaagaagaag
     3061 tactagtgaa aatcacgaaa gcaactcctc gctatgcaga aggagaatta ataaaagtca
     3121 aaaaaacaag caaagatcgc gtagtggcac catgtcctgt ttactatgaa tgcggtggct
     3181 gtcaattaca acacttagct tatcaagctc aattggattt taaaaaggat ctgcttttgc
     3241 aagcattgga aaaattcaaa ccagcaggct ttcgatctta tcaacttttg ccgacgatcg
     3301 ggatggatga gccttggcat tatagaaaca aagcacaatt tcagttacga aaaaataagc
     3361 agactcaaaa aattgaagct ggtttatacg aagcaaactc tcatcagtta gtccctataa
     3421 cagactgctt ggttcaagaa cctcgaacac aaaaagtaat gaatactgtt gttcaactat
     3481 tgaataaatt tgatgcaccc atctatgatg aacggacaaa cagtggaatc ttccgcacaa
     3541 tcatggttag aatcggtgtt aaaactgggg agttacaagt tgtttttatt actcgcagta
     3601 aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa tgaacgactg cccgaagttg
     3661 tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct cgtgatgggg gatgaaacga
     3721 tccacttatg gggaaaagaa gccattgaag aacaaatcaa tgaagtgact ttcgatcttt
     3781 ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga attgctttat ggcgaaggaa
     3841 tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga tgcttattgc ggagttggga
     3901 cgatcggctt aagtgtagca aaacaagcca aagaagtacg gggaatggat accgttcctc
     3961 aagcaatcgc tgatgcaaaa aaaaatgcgg aaagattagg atatcacaac actcgatatg
     4021 aagtcgggac agcagagcaa gttctgccta aatggttgaa tgaaggtttt cagcctgatg
     4081 gtatcatcgt agatccacca cgtacgggtt tggataacgc attgatccaa tcattattga
     4141 aataccctcc aaaaaaactt gtttatatct cctgtaatgt ttctacactt gccagagatc
     4201 tggttaattt aagtaaaaag ttcaagattg aatatttaca gtccgtcgac atgtttcctc
     4261 agactgcacg ttgtgaagtt gtcgtgaaga tgacgagact cgctgactaa atgaaagagg
     4321 ttgtgaaaaa gtctgccctg tatcaagtaa taagaacagc caaacaaaaa tagctcttca
     4381 tgttttatga atactgtact tgtgaccgaa gagttaacag gaggacacta tttatttaaa
     4441 aaaagggttg tgacatagtt tttgtcacag ccctttttga ttattattgg atattcacgt
     4501 ctatcacttg ataaaatgca tttgtcgtat cgctgatatt ccatactgct aaaatcacat
     4561 tatatccttt tcgatcctgc ggaagtgtga tcgtttggtt gactgttttt ggtggaacag
     4621 ttgctttgtc gtctattttt gtgatcaatt caaaatcact aaatttcaaa ggttgattag
     4681 gattccaagt tggtttggta atgtagtaat cccaagtact tgtccgatgt tgtgctgtta
     4741 aattccaagt aatatttaat gttcctggat taaccatgtt tttatgccat ctgttagatg
     4801 tctgttcatc taaaggctca aagccgctga ctcctgcact agcaattttt ccatcaatga
     4861 atgtattttt ggtagcttcg atactttgcg gttcccattg tgctcttccg acattttgat
     4921 ttaagtttcc tgctgctgat gttcctaggt atgcacggct tcctggagaa gtaatataac
     4981 catgcgcaaa acctggaatg ctaaaaccga acaaccctaa actaactaac ccaataaaca
     5041 ataacgtact aatctttttc atgtagaaac ctccttcatt tgataaccac attgtaaacg
     5101 ctttattgta ttaacacaaa cattatgctt ttataatttt atgttgaagt aaaaaaggtg
     5161 aaaaaagtaa gatgcagatt ataacagaag tagacgagaa aagtacaaat agaaaacatg
     5221 agaatcaaat catatgatct ttttcaaaaa catcattcat ataaacaaat gttcaacttt
     5281 ttaataacaa caattatcaa tttttttctt acttcacaaa ctaaatacag gtcttgctgt
     5341 gagccttcat ggtaaactaa tattatagaa aggagttagt aaaatgaaga tgataaaaga
     5401 tattcgtttt tgggtttgtg ttataggaat tgtcatatta ggctttttat cagggttgct
     5461 tagcggaaat cccggaaaat attactattc tttgcaactt ccaccatttg caccacctag
     5521 ctggatattt gggccgatgt ggaccttact gtatatattg atgggtattt ctctttattt
     5581 gttacttaat cacaacaaca aaaaacagcg aaacaatctt gtcggtcttt ttgtgattca
     5641 gttcattttt aattttatat ggtctgctct attttttaat ttaagaaata ttttcattgc
     5701 agcaatcgat attacacttt tagttatttt cttgtcagtg cttatgtatc agttgtggct
     5761 gcatcatcgc ctgacaatgt ggctcatgat tccttattat ttatgggtac tattcgcaac
     5821 tctattgaat tattcaattt attttttaaa ctaagcgcta aaaagagacg tcctgtaatt
     5881 taacacatag aaaaagtgcc acctaagtta gattttttgt ctaatttagg tggcactctt
     5941 tattaaccaa actatttttt tctagtaccc acttgttgtt ccatactcat tattctggtc
     6001 ataaccataa ccattttgtt cctgtccagc gtaaggatca taactttgag tatcttgact
     6061 tggtgtatag tactgacttg tatcttctgt ttgctgataa taggtattcg gatcttggta
     6121 ctgatcatat gtgttttctg aataggaaga ataatcggtg tacccttcac tattcgatcc
     6181 atcaccgttt gtcgaatcgc catcttccga atcatcaaag aacatcattg aagcattctc
     6241 atttttcaaa ttaggtaatg tatcgcttgt ttttatattt aattgtttct tcagttcatt
     6301 ttggatcgaa agaagatcat tttttcctaa aatttgatag tagatatcat tgatcatttg
     6361 tccttcgcct tgcaactggt cttgtttgat tgtttcaaac gccggggtgt agttcgtcgc
     6421 tacatcaagc atgtcatccc aatctaagtc cgtcgtcaca ttgttctcaa ctgcttttaa
     6481 aatttttcta taattgctta cgccatctaa acttaaaact ttattgacga ttttcgtcac
     6541 tacttcccgc tgacgacgtt gtcgaccgat atcgccttct ggatcttcgt gtcgcattcg
     6601 agaataggct aaaccttttt ctccattcaa tgtttgttca cctactggta catggacacc
     6661 atccaaagta aagtcaattt tattattcac tttgatgcca ccgaccgcat tgatcaaatc
     6721 ttccatgcca tccaaattga tcgttacata atgatcaata ggaatatcga gcagttgctc
     6781 aattgaatcc atggccattt cgacaccacc aaatgcgtag gcatgattta atttatctac
     6841 cgttccataa cctacgatat tcgtataaat atctcggtcc aaactgatga tcgttgattt
     6901 tttttgtttc ggattaactg ttactaccat catcgtatca gaacgtcctt gttccgttct
     6961 tcctaatcca ccggtatcta aacctaatag caaaaccgaa aaaggttctt tgtcatcaat
     7021 gcttactctc tcagaacgtt tggaggatat tcgattagag tttttagaaa ttttgtttac
     7081 tgtttggctc gcttctccca taaatctgat tccgtatgct gataatccgg caatcagcaa
     7141 aacgaatacc cctaacaagg ttaatagaac tttttgccat cgcttcataa tacacctctg
     7201 tttcacattt tctaaaatat tgtctcatat aactgttcaa tcagcaaatt cactttttaa
     7261 tcataagaaa actttaaaaa acatttttgt gtaacgatta ttatttttta aaatttctca
     7321 aaattcttaa ctatcatacc ataccgtgaa aaagatcgaa agaccgaata cacgcagttc
     7381 gcaatcgttt tatctttttt tatgtatttt ttccagaaaa agcgaacaac aaggtatttt
     7441 ccaaataatc gtgcagtaaa atgatcgcac atacctttcc catcttttta acagtctttg
     7501 cctaccgttc agattatcat tcgatttcac gacaatttct tcctatgact ggtccttttg
     7561 ttaaacccat cttctattcg ttttatttcc agctcactgc atggctctta aaatgactcg
     7621 aactagtgat tgcattacca tcatctttta atggatagat atgctcctct tcaaagtaaa
     7681 acgattttct tttaaagtaa tagaattgac atggtaatga gtatgtatac tagtggtttc
     7741 acaaatggtc ttttattcaa catccaaaaa ctgaaatttc tttaaaatcc attactaacg
     7801 aagaaaatgt tccttgcttg ataattagtt aggacatgtc aagtgaacaa atgcatttac
     7861 ttagccaaaa aatctaaaaa cttcataaat agacaaaaag acacgactct ctaagaagtc
     7921 gtgtctttat tcaacaaatt attataattt agtcacttca gcagcttgag gtccgcgtgc
     7981 accttcaaca atcgtgaatt ctacttcttg accttcttct aggctcttga agccgtctcc
     8041 ttgaattgct gagaaatgaa caaatacgtc atctccgccg tctactgtaa taaacccaaa
     8101 tcctttttca ttgttgaacc atttaaccgt tccgtgttcc ataaatgtat atcctccaaa
     8161 aaagttcttg caagtaacct tgcaagaaca ttatacccaa tttagaaaac gctagcaagc
     8221 tttcgctctt ttattttcca aattattttt aagactgata tgacggagga aatggttatt
     8281 ttgctgcttc gaaacgtttg tttacttcat cccaattaat aacattccag aatgctgcaa
     8341 tataatctgg tcggacattt ttatatttca aataataagc atgttcccaa acgtccaaac
     8401 ctagaattgg tgtctttcct tccatcaatg gagaatcttg atttgcagta gaggtaatag
     8461 ctaattttcc attttccatt acaagccaag cccatccaga accaaatcgt ccagcggctg
     8521 cttttttgaa ttcttcttta aaagaagaaa aatcaccaaa agcttcatta atcgcttctt
     8581 ttatttctcc tgtaggttcg ccaccagcat ttggtgccat gatttcccag aaaaatgaat
     8641 ggttagcatg tccgccacca ttgttacgta cagctgtctt gatatctgtt ggaatagcgt
     8701 ccatatcaga taataattct tctattgttt tttcgcctaa ttctgggtat ttctcaattg
     8761 ctgaatttaa attcgtcaca taagtattgt gatgcttatc atgatgcaga tgcatcgttt
     8821 cttcgtcaat ataaggttcc aatgcgtcat atgcataagg tagatctggt aatgtgtaag
     8881 tcataaaaaa attcctcctc aatgttttct taataatgtc tacattacaa agagtaacaa
     8941 agagaaggaa attcttcaat tgttatgcct agtcgtcttt ttcactttga cctacttctt
     9001 cttcaaaaac ctcagtttct ttaaaaataa acaacttgct gaaaatatag ttcgcaagga
     9061 taacaataat ttgagtgatg attttcgcca catactcatt gctgttcata acattgatca
     9121 acaataccat tgcccccatg tccaatccgt aagaaaggat acggtagaag aagaacttac
     9181 cgcattcaat aagtgcatgt gtaaaattcg gtgattttga atggaatacc caaagtttgt
     9241 tcgttacaaa agcaaacaat acagaacaaa accacgaaat agaattggat aaggccatac
     9301 ttaaatcaaa atattggcgt gcgagagcaa aactaacaaa atttacaatc gtagccaaac
     9361 caccaaaaaa gagatagact agtatctccc aatatccttt tgcttctaaa tatcctttta
     9421 actgtcgata ctgcttcatg agccaaccta ccttattaaa aattcttatt tatcatactc
     9481 tatatttcaa taactttcat ctttatttaa aaaaattaat tttttttgat cctttgcctt
     9541 tgtctctgct tggatcgtca catgtgtaat accatatttt aattttaata aagaaattat
     9601 acgaggatat atcttttcaa tctcgcttag tagctgatct tcaaaatcaa tatgtccgtc
     9661 gaaaataatt atcttttcgc tatactgcca aatatggaca tgatgaacat cctttacccc
     9721 atcgattgct tcaatttctt gttccatttt ttcatagtcg atagcaggtg cactttgtat
     9781 caaaatagcg gcagcttttt tgatcaccat tatcgcttct atcaatatat atatcgaaat
     9841 gatcagtgta ataactggat cgatccagta gatatcgaaa aaacggatca aaattgctcc
     9901 gataatcaca cttaccgaag acagtgtatc acttaacaaa tgcaaataac tagattttat
     9961 attcaaactt tcatctgctt cttgtcgtaa taataatact gagaacaaat tcgcgagcaa
    10021 tccaataact gctactgtta gcatcaaatg actatttatc ttttctggtg aagtgaaccg
    10081 cttgaatgct tcaacggtta aaaacaagga taatatgatc aatatgaatg agttgatgaa
    10141 agcactcaat atttctaaac gctggtagcc atacgtacgt tttttatttg cttcttttcg
    10201 ggccattttt tgtgccccgt aagaaaaaat aattgccaat gtatcactaa ggttatggaa
    10261 agcatctgaa acaagagaca aactccctga cataatccct ccaataaatt ctgataaagt
    10321 gatcaccgca ttcaaaacca ctactccaaa tagttttttt gatgatctct gtggctgtgt
    10381 aggcactatt ccttcctcca ttttatttat attggatagt ctttagacaa gaataacatt
    10441 tttttgatat ttcaccaatc attcagctgt ttccttaaag cacttgtatt tcacttgata
    10501 cttcgctaga attgcaagtg atgacactac tagatgagga gtacttatga caacaaaaca
    10561 attaattatc agttccttta tacgattcaa agacctaaag gaggcgagaa atgtcccatt
    10621 ttggaaagcc attgtctatc ttttagtttt gagtataatt atggctttgc caatctcttt
    10681 tcaagttttt caagtcttaa acaacattca attggatggg caaaagattg ctactcgcat
    10741 tcctgatttt acaatccaaa acgggcagct tcaaaccgaa gaaaaagaag gattcattta
    10801 ccaaacaaac tccattatct tcacatttga tccagaggga aaacgaacag aacaggacat
    10861 ctctactgat ttgatgggga attttgttag tgtcgggatg ttaaaaaaca aacttatcat
    10921 agctcttcct aatacaggca cgacttctgc tttgttgaac aataaccaat tagaacttcc
    10981 ctacaccaat gaatctttaa aaaacttaac aggtaagcag ctccgttctt ttttaagtga
    11041 agcatctata ccactttgga taaaagtcct gacatttata ttttctattt atcctagttt
    11101 tctgaatctg ctgatcactt tgctatttac caacattgct gcttttctct atgcacgctt
    11161 tcgactaaca aaagctactt ttcttgattg tttaaaaaca ttgatctatt caatttcatt
    11221 gcctactata attgcaacaa tcttgatggt ctttcttcct tcttttgaca ctagcgcctt
    11281 cattgctttt gcaggaatct ttatttttgc tcaagctgtc aaaggatggg caaaaatatc
    11341 gatcaattaa gaaataaaga caactcgtta tttctacttc aatagaccct tttagaggtt
    11401 gtgacatttt tttgtcacag cccctttttt atttgaaaaa tatttctttc tcaaaaaaca
    11461 agcaaggcgc ttcaaagttc ttcttgtatg cgctacactt agagtaaaga aaggcgggaa
    11521 attaactatg tcagaaagaa tcatttatgc agatcgtcca tttcgtgatg aatttatcga
    11581 agaaatccat actatagcgc ctggttatat ctttaaaaca gaattacaac cagaggatct
    11641 acaaaatgta gagatcagtt tgggctggaa ctcagactac caagaaaaac tattagcctc
    11701 ttctaccttg aaatgggtcc aatctatctc agctggtgtt gatcagctac cccttgagac
    11761 tttttcaaag aaacatattt tactttcaaa tggtagtggc atccacagtg aatcgattac
    11821 ggagcatgtc ttaggaatca tccttgggta ttctcgtggt ctttttcaag cacaaaaggc
    11881 acagagtgac atgaaatggt taggttcatc ggtccattac caatctatcc aagggaaaaa
    11941 aatactaatc attggaactg gccatattgg aaaaatgatt gcaaaaaaat gcaaagcatt
    12001 agacatgaac tgctatggaa tcaatacatc cggtcatcca gcagaaggaa tggaaaagac
    12061 tttttcttta aatcaactaa aggaagttct tcctgaagta gatttcgtag tcaatatctt
    12121 acctctaacg gaggaaacaa ctgggctctt tgatcagcat atgtttgaac actttgatcc
    12181 aaaatctgta ttcatcaatg ttggccgtgg tgcttctgtt aagacacaag atctggtgca
    12241 ggctttaaat aaccagcaac tttcctttgc agcattagac gtttttgagg aagagcctct
    12301 tccacaagat catcctcttt ggaaaatgga caatgtactg attacttccc acattgcagg
    12361 tttgacacct gattttcaaa agaaattaat ggctattttt ttaaccaatc tgaaaagcta
    12421 ctttgccacc catgagcttc aaaccaatca agtaaaattg tctgcaggat attaatagct
    12481 gaaaacaata ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca ccctctcttt
    12541 ttttattgtt ttttaaagtg ataggttggc tcctccaggt tgggatcata atcaacaagt
    12601 aaatcatatc ctttaaagaa ccattcatct gcttcctcga taaaaaaaag tgtcccatcc
    12661 atttccactt tctttacagg atgttctggc tgttcaactg acattccaat cgaaaaacca
    12721 tcatgaacct ctgttttgcc gtagactttt ccaaaaaaac gtattccgta agtttctggc
    12781 agatcaattt ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa tttcatccca
    12841 acaacctact ttctcttttc tttctattga ttcatcttat cgtctagaat atccacatcg
    12901 cctgtctctc caataaccag tagattatca ttttcacgaa catattcatc tgctgcagga
    12961 gaagcgataa ccgtttgatc tactcttcgt atggcgacta ctgttaagtc aaaacgttgc
    13021 ctgaaattta attcagctaa tgttttgttg aaaaatttag gattggagac tcggatttct
    13081 gctaaagaat atttatctga caactctagg taatctaaaa tattctttga cactaaatta
    13141 tgcgcaatcc ttattcccat gtctcgctcg ggatggacga cgcgatcagc tccgatcttt
    13201 tcaagtactc tggcatgata ctcattttgg gcttttgcta aaatatttgg tacgcccatt
    13261 tcctttacca tcagagtaac taagatactg gcttgaatat cttctccaat agcgacaatt
    13321 acatgatcga aatttcttat gcctaatgat cgaagtgtca tttcatcctg tgcatttgcc
    13381 acaacagcat gagtggctat attcatatat tcattgaccc gatcttcatt actatcaata
    13441 gccagtactt cttgaccagc ttcgatcagt gttcggcaga tacttccgcc aaatcgtcca
    13501 agacctacaa ttgcaaaatt ttgtttcacc gttttcctcc tttagtatct tcttttctca
    13561 gtataacaaa gcctttacaa aaacaaaagc aagcatcatt attgtaaaaa agccaagaaa
    13621 aataatagtt attttctcag cttttgatta tcctctttat catcgttttt atttcaccaa
    13681 accatgtttg aatgcataga tagcggcttg tgtacggtct tccacttcta gcttagccaa
    13741 tatattggaa acatgtgttt ttactgtttt cagtgtaata aacaattcgt ccgctatttc
    13801 ctgattactt tttccttcag aaatcagcat taaaatttca ttttcccgat tcgttagttc
    13861 ttcatgtaat acaggctcat ttcgccgact cattttttcc atcatttttg ttgtcacttc
    13921 tggttctaat actcgctcgc cacgttgcgt tgcccttata gcatcagcaa tttcatgtgc
    13981 ggtagaggtc ttcaacaaat accctgctgc acctgcttca atggcaggat aaactttttc
    14041 gtcatcaata aaactggtta cgataatgat tttagcttct ggccaatctt ttaagattgc
    14101 ttttgttgat tcgataccat ccatttcttc catgactaag tccattaaaa tgacgtccgg
    14161 tcgaagtgtc atcgccttct catagccttg tcgtccattt tcagcttctc caattacttc
    14221 aatgtcttct tgaatagata aataagaaga gacgcctaag cgcaccattt catggtcatc
    14281 tactaataaa acttttatca tccttatcta tgcctccttc atcaaaggga ccttgatttc
    14341 tacacttgta ccttgacctt taaaactaat gatttttact gttccaccaa taccagctac
    14401 tcgttctttg atattgttca atccataact tcccgttttc gtttcattca tattaaaccc
    14461 tgttccgtcg tcaataatac gtaacaaaag attattgtct attttgtgca aatatacctc
    14521 taattcattg gctttagcat gtcttaatgt atttgataac aattcttgaa cgattcggaa
    14581 caagtgatcc tcaatggaac tagatagttt tacatcttct acatcccatt tcagcgaaat
    14641 ctggatttta ttttgcaatt ccttcaataa ctgctcgata ccttgcttta aacttttctc
    14701 ttctaaattg actggacgta gatgaagaag cagcgcacgc atctcagact gggaagcgtt
    14761 gatgatctct gctaccattt tcaactgctt gcgaaacatt tctggtgtct cgcttttttc
    14821 tgcttgctcc gtcaatgccg acatcatcat cattgctgca aacaattgtt gcgaaactga
    14881 atcatgcagc tcacgggcta atcgatgtct ttctaattct aaaatttctt ctttggtttg
    14941 tccatctaca tattggggac ggctcgttac aatttgtaat tcactagata cttcaatcat
    15001 tttttctttt atcttagtga tttccttatc aatatctttg atgtataaat cgtccgatgc
    15061 acttgggatc ggaagaagca acaatttgga ttcataattg ccagaagaaa gtgcacgcag
    15121 cttttcttcg attttcccgt attttgtctt ttgaataagt gataataata gaaagacaat
    15181 taatcctaca cctaaggaaa tggctaacaa atggaagata agcggaacat ataataaacg
    15241 tgcacgcagt aattctcccc accaatgact ttgattgctg gcataaaaat aagtgaaaag
    15301 tgaaaataga ataataagga aagccgcaat ccccgaataa acagctagca tcgctctgga
    15361 tatttttccc atcatattcg tatcacctcg acatctccaa tcaaggtatt agtaataatc
    15421 ttcaatctac gagggttttc atcataatca ctgctgtaga ttttgatttg ttcatttttc
    15481 aaagaaaatt gctcttcttc aaacaagaca tttcctacta atgtcgcatg ctctaatcgg
    15541 atggctacgc ccaaaggtac taagatccgt gttctgccta tcccttttct tacaatcaca
    15601 atattatcat cttttggcaa aagcgtattg cccaaatcta tgatagtatc accagaaatg
    15661 acggcgatgt tgatatcgtc ccattcatag acgtcgtttc caatacgttg gttaccgaat
    15721 aattgctgtc ttttccgctc attattatgt gtttttaatt gctctgtttg gatcatcatg
    15781 atctgtttct ttcgccaaaa agcatttttg gttaaatcta tccctgaaat ctctacacct
    15841 ttcagaccaa taaataaaac ggcaaataca gccatcatcc ataaagcagg actgttgact
    15901 aagctaaaaa aaatgaccaa acttcctaat atcaattgaa aattttgaaa tttagtacga
    15961 ggatgtttgc gcatagctag atatatattg aaaataccga atatcactaa aagcaaaagt
    16021 ccagtattgt ttacgatttg ccaaacagca aataacagca gtagtgcttc aactaccaca
    16081 aaaaaacgcc aagaactatt catcgctctt tctccctcct gacacattct catttcatca
    16141 tacctatttt tttaactgtt ggctatagga ctttagatgt aagatcgttt gttgaaatct
    16201 aaaatacaca aagagaaaaa gatctagagt gtatcatcag taagatgaac tctctagatc
    16261 tttcagtcag actacgcttg ttctactttg acgattttaa ctaacatatc gccacctgga
    16321 gtagcaatag ttacttcatc atttttacgt ttgccaatca atgcttgcgc aataggagaa
    16381 tcattggaaa ttttgccaga aaacggatct gcttcagcgc ttccgacaat cgtgtactct
    16441 tcttcttctc catctggtag ttcgatgaat gtaactgttt taccaattga tacctcatct
    16501 gcatctacac cgtcgttatc gatgatttga gcaaaacgaa tcatattttc aattgttgtg
    16561 attcttcctt caacaaatgc ttgttcatct ttcgctgatt catattcaga gttttcggaa
    16621 agatctccga aaccgcgggc aattttgatt cgttcaatga tttctccgcg tttgactgtt
    16681 tttagttctt ctaattcttt ttctaatttt tctttacctt ctagagtcat tggataaact
    16741 ttctctacca tgtgtaacgt ctcctttttt cgataaaata ccgcaaaagg taatcttttg
    16801 aggaatatag tccaaaaaag attaccacgc ttttcttcaa atgtaaatgt ttttctaaaa
    16861 atgatcaatg tctagagcct gtttttctaa gaattattta catatttttc cactaattcc
    16921 atatgttctt cataagtttt agcgaaataa acatttccag tgctcgtatc tgctacgaaa
    16981 taataatagt cattttctgc tggttctaat actgccttga ttgcctcttc gcttggattg
    17041 tcaaaaggtc caggtccata gccggtattg gtatacaaat tatatggtga atctacagct
    17101 gtatcttcat acgtcactaa ttctttgtgt tcacctaatg cgtataaaac agagatatct
    17161 gactgaagtg gcatatttgc tttgattcgg ttaaagaaga cttgtgcaat gtttttacga
    17221 tcattttctt ttacgccttc tttttcaact agagaagcta gagttaaaac ttcttgtaca
    17281 gtcaaatttt tctgcttgat agctgaataa tagttttgca tgaccgtatt cgtcttgttc
    17341 accatttcaa tcactagatc cttaagcgaa gtactcgtgt aataatcata tgtggctggg
    17401 aataagtaac cttccaaacg gtattttgtg ttctcagctt tagatgcact ttccaacagt
    17461 tctgggaatt tttggtgcaa ctcgttaaag aaagtctcat cattcactaa atctaaaaat
    17521 tctttcttgt ctttaccagt taccttcgct acccgttcag caatctgatc gatatcgtac
    17581 ccttctggaa tggcaatttt cccatctgca acttttgttg gctcagaagt accgccttct
    17641 tgcaactgtt tgccaatctc gtcaagagtc atattaggcg ctaactgata atagccagct
    17701 tgaaagccag ttaaattttt aaattttgta taataattga aaacaatccc gcttttaata
    17761 atattgtcct tctccaaaat ctcaccaatc tgcttgttag aagagccact cgggatctcg
    17821 acttggacta attggtcatc tgatttatct aaaggcttca acccgctatc tacataacga
    17881 tacacggtaa atccaagaaa tccgccaata atcaataatg taagagcaat cactaaaatg
    17941 atcttcctca caatcttatc ttctttttta cgcagatttc tccgcttgtc gattatatct
    18001 gctgtagctg ccttactggc ttctttttta tctttacttg ggtgttgttt gtttcctgac
    18061 aaaacaactc ctccttacta tcaagttcgt aactttattc attatacatg atatttgaat
    18121 tggcgtgaat gattttattt aaatattcta tcaaaaatgt aagtagtaaa tccatt
//
LOCUS       DAZUKR010000045        16520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000045 DAZUKR010000000
VERSION     DAZUKR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.045"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..754
                     /locus_tag="ACG89C_001398"
     CDS             <1..754
                     /locus_tag="ACG89C_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4899944.1"
                     /translation="VIHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYNQ
                     QEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENPE
                     NMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVFT
                     MEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHA
                     SYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1328..1897
                     /locus_tag="ACG89C_001399"
     CDS             1328..1897
                     /locus_tag="ACG89C_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS4899945.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1884..2744
                     /locus_tag="ACG89C_001400"
     CDS             1884..2744
                     /locus_tag="ACG89C_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4899946.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2934..3638
                     /gene="yycF"
                     /locus_tag="ACG89C_001401"
     CDS             2934..3638
                     /gene="yycF"
                     /locus_tag="ACG89C_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS4899947.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3643..5478
                     /gene="walK"
                     /locus_tag="ACG89C_001402"
     CDS             3643..5478
                     /gene="walK"
                     /locus_tag="ACG89C_001402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS4899948.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5475..6791
                     /gene="yycH"
                     /locus_tag="ACG89C_001403"
     CDS             5475..6791
                     /gene="yycH"
                     /locus_tag="ACG89C_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS4899949.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6792..7661
                     /locus_tag="ACG89C_001404"
     CDS             6792..7661
                     /locus_tag="ACG89C_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS4899950.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7716..8525
                     /locus_tag="ACG89C_001405"
     CDS             7716..8525
                     /locus_tag="ACG89C_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4899951.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8628..9257)
                     /locus_tag="ACG89C_001406"
     CDS             complement(8628..9257)
                     /locus_tag="ACG89C_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4899952.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9427..10719
                     /locus_tag="ACG89C_001407"
     CDS             9427..10719
                     /locus_tag="ACG89C_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4899953.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10795..11457)
                     /locus_tag="ACG89C_001408"
     CDS             complement(10795..11457)
                     /locus_tag="ACG89C_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4899954.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11682..11966
                     /gene="groES"
                     /locus_tag="ACG89C_001409"
     CDS             11682..11966
                     /gene="groES"
                     /locus_tag="ACG89C_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4899955.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12017..13642
                     /gene="groL"
                     /locus_tag="ACG89C_001410"
     CDS             12017..13642
                     /gene="groL"
                     /locus_tag="ACG89C_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4899956.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13731..14870)
                     /locus_tag="ACG89C_001411"
     CDS             complement(13731..14870)
                     /locus_tag="ACG89C_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4899957.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14981..15565)
                     /locus_tag="ACG89C_001412"
     CDS             complement(14981..15565)
                     /locus_tag="ACG89C_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4899958.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15692..16123)
                     /locus_tag="ACG89C_001413"
     CDS             complement(15692..16123)
                     /locus_tag="ACG89C_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4899959.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16141..16461)
                     /locus_tag="ACG89C_001414"
     CDS             complement(16141..16461)
                     /locus_tag="ACG89C_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4899960.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         5405 a         2824 c         3363 g         4928 t
ORIGIN      
        1 ggttattcat tatcacgcat ttatacgcaa agctcttcaa gaggctgtta ttacagaatt
       61 gataagtaca aatgtagcag ataaagtaag aaagccaaaa aagaaacaat ttgtttctca
      121 agtttataat caacaagaga tcttgaaatt acttgacatt ttgtcagaag aaaaactcta
      181 tttggtagtt cttttaacag cattttatgg attgcgaaga agtgaagttt taggattaaa
      241 atggtctgct attgattttt taaacaaaaa aatcatgata aaccatgtaa tcatagaaaa
      301 tccagagaat atgtcacagt taataaagaa agatcaaaca aagaactctt ccagctatcg
      361 tacattacct ttggttgaaa cgattgaacg agcattagta aatcaagcta aatggcaaca
      421 agataatcgt attcttttag gagatgatta tattttagaa gataatgagt acgtatttac
      481 tatggaagat ggacggttga tgaagcctca atatgtaacg catcgtttga gtaagttgat
      541 taagaaaaat ggattgaaaa aaattcgctt tcatgacttg cgtcattcta atgcttcaat
      601 aatgttagat agtggccaga atatgaaatc cattcaagaa tggctgggac atgcatcgta
      661 ttcgacaact gctaatttat acacgcattt aaccgcagga gtaaaagatc gtgcagcaga
      721 agcattagaa aacgcatttg ggtttaaaga ataaaaaaac aaaaaacaat atctttgggg
      781 tatcgcttct tgtttttaga atttttttgt tggcaatttg ttggcagtat gttggcaatg
      841 aaaaaattca caaactaaaa acctatttat aaagctttat aaaacttttt tcacaaatag
      901 agcatttggt taagagtcag ctgctctacc aactgagcta acggcccgta caaaaagtac
      961 ttaataatag tagcactaag aattttaaaa tgcaagacta catttaaaga aaatcagaaa
     1021 aatctttact tccttatagg aaatcattca tagattattc acatttgctc gctattatat
     1081 aagcatacca acaaacaaag ttcttgcaag agaacaaacc ctaacacttt tttcattttt
     1141 tactcctttc ccgccgtctc cccggcgggt ctcctgttct taacgataaa agacaagaag
     1201 gcttagcctt cttgtttttt gttttcatgg aatcccgcta atcaaatcac ctagttaaag
     1261 caactagatt gaatgtttat cataagtctg atatcatgtt tatgaaatga aaaaaggagc
     1321 ggttatgatg gaaaaaatgc gtcagcaatt aatggagtgg gggaaggaac tagaaacatt
     1381 ccgtctgcct cactgggaag aactccctga cttagaactt tatatggacc aagtcagaac
     1441 gttagttgat cgttatcttt cacctgtgat acaaggagaa aagcatccat tgcttacctc
     1501 ttctatggta aacaattacg ttaaactagg tttgatacca gcaccagtta aaaaaagata
     1561 taacaaagaa cacgtagcat ttttacttgc aatcactacg ttgaaacaag tattgactat
     1621 tcctgaaatc aaagaaggga tattgttcca aggaaaaaca gtaggaattc gtgaagccta
     1681 taatctattt tgcgatgaac aagaagcagc tgtgtggatg gtcagccaat tagcacaagg
     1741 taaatctcat ccgcaaaaat tcgaccaggc aacccctgta gaatatattg ctgtaagagc
     1801 ggcaacttta tcgtttgcaa tgaaacttct tgcggaaaaa acgattgttc tagaaaccga
     1861 atatttaaaa gaggagaaaa caaatgaaaa acaataaaat cgcaatttta gtagattcag
     1921 gtacggatgt tccagaagaa ttgataaaaa aatacgagat gtatatgatc ccattgaaaa
     1981 ttatttataa agatcaggta tatacagata aaattgacat tacccctgaa gagatctatc
     2041 aacgtttgcc agaggaaatc cctggtacat ctttgccgga tggtgagtcg atcacagaaa
     2101 tctttgagca aatcaaaaaa gacggttatg aaaaggtact tgcggtaaca atttccagtg
     2161 gattgagcgg gacatataat gtggttcgtt taattgcaca acagcaagaa gaattggaag
     2221 catatgtact agatacgaaa aatattggta ttggtgcagg tttttctgcg atccaagcag
     2281 caaaatggct ggaagaagga gtagaatggg atcaactgat cagtaatctg aatgagttag
     2341 tcgaaagaac aaaagttttc tttaatgttg cgacattgga atatcttcaa aaaggtggac
     2401 ggatcggttt agttgcttct attgtaggaa ccgctttaaa gcttaaccca atcatctcat
     2461 gtaatgaaga agggatttat tacacagtag gaaaagctag gggacgtaaa aaaagtttgg
     2521 ataaaacggt tgcttacgtc aaagaaaaaa tcggtaatgc aaaggtcttc aacttagctg
     2581 ttgcccatgg ggatgcgaaa gaagaagcaa ttgaaatgga agcgagactt aaaagagagt
     2641 tcccacaagc aaaccaaatc tactttggcc agatttctcc ggcattagta gtccacactg
     2701 gtccgggatt attgggtgtc ggtgtacaag ttttagaagg ataaatcaaa aaaaggaaac
     2761 actgaggtga aaatcagtgt tttccttttt tattgaagtt agaacaaaaa ataataaagt
     2821 attttgcact ttcattttaa ttttctcttt tgttttatag gcaaagatga aacaaaagca
     2881 gaaatacggt ataatgagaa ggtatgacaa taatatataa gaggtggctc tttatgaaaa
     2941 aagttttagt tgtcgatgat gagaagccga tttcggatat cgtcaaattc aatttagcca
     3001 aagaaggcta tgacgtttat actgcatatg atggggaaga agccttggaa aaagttgctg
     3061 aggtagaacc tgatttgatt ttacttgatt tgatgctacc taaaatggac ggcttagaag
     3121 ttgctcgtga ggtacgcaaa acttatgata tgccgatcat tatggtaaca gccaaagatt
     3181 ctgaaattga taaagtccta ggcttagagt taggcgccga tgattacgtg accaagccat
     3241 tttccaaccg agaattagtt gcacgtgtca aagcaaatct tcgtcgcggt gccacggctg
     3301 cgaaggaacc agaagaggca gcacctgcgg aattgacgat tggtgatttg acgatccatc
     3361 cagaagctta catggtgacg aaacgaggag aaacaatcga gttaacacat cgtgaattcg
     3421 aactactctt ttacttggcg aagcaccttg gacaagtgat gacaagagaa catttattac
     3481 aaacggtttg gggttatgat tattttggag atgtacgaac agtggatgta actgtacgtc
     3541 gtttgcgtga aaagatcgaa gataatccaa gccatccaaa ttatttagtg acacgtcgtg
     3601 gtgtcggtta ctatttgcgt aacccagaac aggagtaatt ggatgaaaag aaaagtccgc
     3661 ttttttcgat cagtcaattt taagatcgct attaccttta ttctgatttt attgatctcg
     3721 atcgaaatta ttggtgccta ctttatacgt ggtttagaac gttccacgat caatacattt
     3781 attaaagata tgaatcaaac ggtggaatca ttagcaacaa caatcagccc ggaattgaat
     3841 cgaaaagata atgcagatga tgaagaggta aatgcgaata tcaaaagatt tattgaaaac
     3901 agtgcgacta gcgatatcat cgaaatacgg gtcgttgacg aaaaagggat catccgagga
     3961 acaaccgatg tcaatgaaca aagcgctgtg ggaaagaaaa atgattatgt tgatatcaat
     4021 gatttcacga ctaaacgtta tgttgcttta gataacgacg ataaacgggt gaatatcaat
     4081 gttcaaccga tcctgtcccc gaccggcgat gccgttatcg gggctcttta cgtcaaaagt
     4141 aatatcgaac aaaaatattc ggaaatcaat aacacagcag tcattttctt tactgcctca
     4201 ctaattgcgg catttatttc aatgatcgta tctgttttag ttgctcgttc gattacacag
     4261 ccgatcggtg aaatgagaga acaagccatt cgaatcgcta gaggagacta cagtcggaaa
     4321 gtcaaagtgc atggacaaga tgaacttgga cagctggctg ataccttcaa tcagttagct
     4381 gaacgtattg aagaagctca agatacaatg gaagctgaac gcaatcgttt ggatagcgtg
     4441 ctatcccata tgacagacgg cgtcattgcg acagatcgca ggggtaaagt cattacgatc
     4501 aatgatatgg ctgtttcctt gctagatatc aaaaacgagg aagcaatcgg acaatcgatt
     4561 ttgacacttt tagatatcga agaagaatat acgttgcgga aattattaga agatccaaac
     4621 gaaatggtac ttgaccgttc aaccagtaca ttggaaagtg atcagatgat cttgcgagta
     4681 gacttttcta tgatccgtcg cgaatcaggt tttatcagcg gattagtagc tgtcttgcat
     4741 gatgtgacag aacaagaaaa aacagaacgg gaacgtcgtg aattcgtctc taatgtttcc
     4801 catgaacttc gtacaccgtt gacaagtatg cgtagttata tcgaagcatt gagcgaaggt
     4861 gcgtggaaag acgaggaagt agcaccaaat tttttgaagg taacgctaga tgaaacagac
     4921 cgaatgattc gaatgatcaa tgacctctta aatctatctc gcatggatac aggtaatacc
     4981 cagcttcaat tagaatatgt caatttcaac gagttagtga attttgttct tgaccggttt
     5041 gatatgatgg tgggaaatca ggaaaaaaat tataagatcc gtcgtgaatt tacgcaaaga
     5101 gatctttggg tcgaagtaga tacagataaa atcatccaag tagtcgataa tatcatgaat
     5161 aacgcgatca agtattcacc agatggggga acgattactt gtcgcttatt agaaacgcat
     5221 aataacgtga ttttaagtat caccgatcaa ggattgggga ttcctaaaaa agatctgaac
     5281 cgagtgttcg agcggttcta tcgagtagat aaagcacgtg caagagcaca aggtgggaca
     5341 ggacttggat tagctatttc tagagaagtg attaaggcgc atcgaggcgc tatttgggca
     5401 gagagcaagg aaggaaaagg gtcgactttt tatatctcgc tgccatatga accttatgag
     5461 gaggaatggt gggaatgaga atctctgaaa aagtcgttcg tataggactc attttgatgg
     5521 ttgcgttaag catatacttc tcttatgcaa tctggctgag cccagcagga aaaagttcaa
     5581 tcaacgtaga tgagacaaac actcaaatga tcgagtctca aagcaatcga aaaacttccg
     5641 aagtctttct tccgcttcat gttacatggc accattcggg aatgatccaa gaaaccaaca
     5701 gtgaaaatct cgtgtaccga ttgcaattga tcatcgataa agcaagattt ggaaagcttt
     5761 ctgaagtagt ttcaggagac aaatcacagt ttgaacaggt gaaaaatctg aactctggtt
     5821 ttgagttggc ctataatgcg ccttttttgc taagagaata caaggaagca tttgatttag
     5881 atttagatgt gtcgaacttg gcaaaagaaa gcgaaaatat gtattttact agaatacaat
     5941 tcgatcaagg aaataaaaaa atccgatttt tgaattattc gaaccatctc gtatatgaag
     6001 caaatatttc gttgaattgg acagatatca ataaagaatt aacagcaaaa gacacaaaat
     6061 ggacagaaat gacaacagaa ccatcgacga tcgacacgca atatgctacg aaacattcga
     6121 tcaaactgaa gaagtatagt tatatcctgt cacagcaacc ctatacactt tttagaaatg
     6181 cttttttcca gaaaccagat agcgtgaaaa ataatgatga aagcaatgaa ttgatttttt
     6241 acggtgggaa tgaaacaatg tctatccatt cggaaacgca agtagttgat tttcgtggag
     6301 agctgccaga gaaaaaagaa aatgctagtg tattttctga aagtttcccc tatattagca
     6361 aattaggcag ttctttaggg aacctcagat attttgaccg cagtgacaat acgatcgatt
     6421 atcggatatt cgtggaaggc ttcccggttt ttgggacaga cagcagagga cagattggtg
     6481 tggaagttgg cggtgaaacg aatggttctg tgcaagtgaa tatccaaacc agtttgaata
     6541 cgattcaagt gccgatacct tcagacgaag aagtagaact tgcaagtacg aatgaaatca
     6601 tccagcagtt aaaagctaag ggagcagata gcaacaagat tggttcagtg atcgttggct
     6661 atacatggca aaatgtcaaa gaaacgaatc gtgtcgttga tctcttacct gaatggtatg
     6721 tgaaatataa agataactgg tattcagcta acgaccttct ggaaaaatta cctggattgg
     6781 aggcgaatta attggatttc aaaaaaatcg aatggatatt tttcgtcgct tttttagggt
     6841 tgaatgtgtt tttattcagc agttatcatg acgcggtata ccaagaaaac aatgtcattc
     6901 gctctaatca gactgaatca atccaacaac gtctggctag cgacgatatc acttacacgg
     6961 ggaagatttc gtctgagaac aagcaaggct attatcttag cgcggagcag accaatctta
     7021 gcacttcatt agcgaattat cgcaaggctg gtaaaagcgg cttgctggca agtggtgtgt
     7081 ttttagacgg tgatgttttg acgaaatctt tatccagttc agatgctgaa aagaacgtga
     7141 tcgatccaga taaaattagt agttccttgg aagatttttt atctaacaaa gacaatattt
     7201 tattcggtaa tgattatgtg tacacgaaaa acttttctca tttagaagaa gaaccactag
     7261 aaatccaagc aagccaagag tatgaaggca tcccattcaa tgatgataca gcaaaaatcg
     7321 ttctttcttt agaaaaaaac gataatgatt atagcatcac gaaatacatg cagacgcatt
     7381 tatctgatat cgagcagctg cgcgaaaaga cggaactgta tacggaagag gaagcaatca
     7441 atacattgta catcaataac aaaataccaa gaggatcaaa aattttatgg cgtcagctag
     7501 cttattcctg tattctgaaa gtccgggaaa aaaatgtgta tgttcctgtt tggcatgtag
     7561 cgatcgaaac aagcgataaa gtggtccaaa ttgagagcgt caacgctttc agcaatacga
     7621 tcgtgacaaa caatactgta ccaaaagtgg aagatcaata aaaaagcgag tataatgaaa
     7681 aaagtttaca aagtaagaag ggaagtagga gtcaaatggc tgaagaaaaa ccagctttta
     7741 aaatcagtat cctagcaagt ggaagttcgg gtaattcact ttatatcgaa agtgaaaaaa
     7801 aacggctttt ggtcgatgca ggacttagcg gtaaaaaaat cacttccttg atggctcaaa
     7861 ttgatcggac accggaccaa ttagatggaa tacttgtgac gcatgaacat cgagatcata
     7921 tacatggcgt gggtgtcttg gctcgtaagt atcatttgga tgtttttgcc aatcagcaga
     7981 cttgggaagc gatggaacca ctgattggaa atgtaccagt ggaacaaaaa catctatttg
     8041 agatggggaa agtcagaaca ttcggcgata tcgatattga gagtttcggc gtctcccatg
     8101 acgcagcggc tccgcaattt tatcgtttcc ataaagaaaa tcggtcattc gtcatgttga
     8161 cggacaccgg atattgcagt gattatatgc gaggtgtcat tgaaaatgcc gatggttatt
     8221 tgatggaaag taatcatgat cttgagatgt tacgcatggg accttatccg tggaatttaa
     8281 aacagcgtat tttaggagat agaggacatc tttccaacga agacggtgca cttgtcatga
     8341 ctgatgtgat cggtaatcgg accaaacgaa tctatcttgg ccatcttagc aaggaaaaca
     8401 atatgaaaga acttgctcat ttaacaatgg aaaatgtttt gaaagaaaaa gattttggcg
     8461 ttggacatga ttttgaaatc tatgatacag atccagatga agcaactgag ctattttcga
     8521 tttaaacagt tcaatcaata aaaatataaa aagcagaaga taaaatcaat cagggttggt
     8581 tttatcttct atttttattt ttttagcata ttaaaaaaat aaccgtatta tcgactgact
     8641 acactgatgt gctgcttttc atgtttctca tccagtatct catctactaa tgcaatcgcg
     8701 taatctgcca tgctgatcgt actgtcgcta gcagcgtttc tttgtaaata atcatcactt
     8761 aacaaataag tacctgtacg tttgccatgt ggatcaaata aagaagctgg actgatataa
     8821 gtccaatgta gatttttaga ttgtttcaat tttaacaatc cttcgtacat attgaaagct
     8881 gttggataga agggtgcatt tttaggtact tggtcaatca tacgagtcgt cttttctgta
     8941 tctaagaata acgaagaggc acccccaacg acaattaagc gtgtatcggt tccttcaagc
     9001 atctcaatca aatgggacaa agaagtttga tgcatttctt cttttccttt gggagcgttg
     9061 aaagcatcaa cgacaacatc aaaagatgca agatcagtag ttgtcaactc aaataaatcc
     9121 ttttgtataa acggaacatc tgatggcaat ttttctggat gacgcacgat tgccgtcacg
     9181 tctaaatctc tggacaatgc ttcgtctaaa ataaatgaac ctgcatggcc agtagctcca
     9241 atgagtgcaa ttttcataaa aaacctcctt ttttattcct gtccattgta agcagttgct
     9301 gaatattcgt caaagaatcc gatttttgaa aagctcagtg cgcattcctt tcatggttcg
     9361 tgatcattgg gtatagttaa attaagaaaa ctgattgata aaaatagaag ttgaaggaga
     9421 taagaaatga aagagtggtt agatcgtgcc tttttaccaa tgataacatc tgttgtgaat
     9481 cttagaccct taactgcttt gcgtaatgga atgacttaca cgatcccaat aattattgtt
     9541 ggctcgctgt ttttcttatt tgctcatttt cctgtggatt acatcgctca gtggatagac
     9601 aaaacaggat tttctatgta ttttacacaa gtataccagg ctacatttga tgtgatagcc
     9661 ttttggatcg tttttgccat tgcatacgct tatgcaaaag aagaagacat cgatcgctta
     9721 ccagtcggca taacagcctt gtctgcttat ctactgctta ttcagccact aaatactcag
     9781 atagatactg tctggacagg tgctgaaggg atactaggag cagttacaac tgggctcatc
     9841 atcggatggg gctatgcacg tttagtaaga cttgcgaaag ggtggaagca aagagagaag
     9901 atacctgaaa atgtttggca gtcttttact tcattgattc caatcattgt tatagtcact
     9961 tttacgctta tcggttcagc ggtttttcaa catttgttca gaatgactgt tgttcaagga
    10021 gtatggacat atgtccagct tcccattcaa tcgttcatag acacgttgtt tggtgtgctt
    10081 ttattaggtt tttttgttcc gtttttatgg ttatttggtc ttcatggatc tgcgatggtc
    10141 aatgggttgg ttagtcccgt cttgcaagcg aattcattag caaatgcaga aatcttagcc
    10201 tcaggacaag agttgacggt ggcaaatggc ggacatatcg ttactcagca atttctcgat
    10261 cagttcatga cggttacggg tgctggattg acgttagggg ctgtattttt tatgacggtt
    10321 tttgcgaaat cgaagaaata cagagaatta ggaaatcttg ctcttttacc agcctttttc
    10381 aacatcaatg aatcaattat tttttccaca ccgatcgtga tgaacccgat gatggctgtt
    10441 ccttttattt tcgcaccggt cgtttcagga ttgattacct attccgcttt atattttgga
    10501 ttcgttcctt tatttacagc agtccaagtc ccttggacaa caccgccgat cctctcgggt
    10561 ttcttagtag gcggagtgcc tgcagcgatt ttacaaggta tcgtgttgtc aattagtttt
    10621 ttcatttatt tcccattttt gaaaaaaata gattctgcaa actgcaaaaa ggaaagacag
    10681 acaaaaaatg atggagcagc agaaaagatg aacgactaaa aaacaaaaat actcctgtcg
    10741 ctttagattg ggaaaatcac caatctagga tgacaggagt atttatagtc atgtttatac
    10801 tagccctttt tggacagcta attgaacaag aacaactaat gtgttcattc ctacgtgtgt
    10861 gatcatcgat gtccagatcc gaccagttgc tttgtactct aggctgaaga agaaacctag
    10921 gaaaaagtag attagcagat gaccatcatt atgagcaaac gcaaacagca aagagctgat
    10981 cactgctccg acccaaaaat ttgaatatcg actgatgatc cccagtatac tgcggcggaa
    11041 tacgaactct tccatgatgg gtccagcaac agttgtcgcc aaaataaaag cgggtgcttc
    11101 catgatcatc tggatgatat tttgtgtatt ctcggaaggg gttgctttgc caaacaatac
    11161 agattctaac atgacagcga tgctttgcag aatcaatgcg acaaagattc caaccaatcc
    11221 ataaataaca atctttggag cagaagcttt ttttaccgct gattcgatcg ctaagggttc
    11281 acgttgctta aaataaaaaa tagctaggac aacggcgcct agtatataca tgactgcagt
    11341 tgctgtgatc aattcagacg tagattttac aagtcctatc gaagctagga tcaagggtga
    11401 gataaaaatt agaccatagc aaaaaatgct gaggaagcta tattttttta aagacatgta
    11461 attcctcctt ttaagggata acaagcatgc aaggcaagct tatatcgatt gatttcaatt
    11521 atatcataat ttcaataagc agctgtcaga ataaaatttc ttttctttga tttaaaagct
    11581 tgcaaaaaag agggagaatg agtattatta aaaatgtagg ttagcactcg aaggttaaga
    11641 gtgctaacag attaaaaaaa gagttatcgg agggatttat cgtgttaaaa ccattaggtg
    11701 atcgcgtcat tatcgaagtc gcaaaagaag aagaaaaaac tgtcggagga atcgttttag
    11761 cttctgctgc taaagagaaa ccccaaactg ggacagttgt tgcagtcggt gaaggccgtt
    11821 tgttagaaaa cggtgaaaaa gttcctgccg ctgtaaaagc aggcgaccaa gttatgttcg
    11881 aaaaatacgc cggtacagaa gtaaaatacg aaggaaaaga atatctgatc gtcgcaggaa
    11941 aagatatcat ggcgattgtt gagtaaattt ggaagataaa aaaataatca aaaataatca
    12001 atgaggtgaa tcaatcatgg caaaagaatt gaaatttgca gaagacgcac gcgcagctat
    12061 gttacgcggg gtagataaat tagcagatac agtgaaagta acattaggac ctaaaggccg
    12121 aaatgtcgta ctagaaaaat cttacggctc tccattgatc acaaatgatg gtgtgacgat
    12181 cgcaaaagaa atcgaattag aagatcattt tgaaaacatg ggtgcaaaac ttgtatcaga
    12241 agtagcatca aaaacaaatg atattgcagg agacggtaca acgacagcaa ctgttctgac
    12301 acaagcaatc gttcgcgaag gtttaaaaaa cgtaactgca ggtgctaatc ctctaggcat
    12361 tcgtcgcggg atcgaattgg caacaaaagc agcagtcgaa gaattgcata atatctcaac
    12421 tgttgttgat tcaaaagaag ccattgcaca agtagcagct gtttcttcgg gttctgataa
    12481 agttggacat ttgattgctg atgcaatgga aaaagttgga aacgacggtg tcatcacaat
    12541 cgaagaatca aaagggatcg aaacagaact agatgttgta gaaggtatgc agtttgatcg
    12601 tggttactta tcacaatata tggtaacaga taacgacaaa atggaagctg ttctagaaaa
    12661 cccatatatc ttgatcacag acaaaaaaat ctctaacatc caagatattc tgccattgtt
    12721 agaacaaatc ttgcaacaat cacgtccatt gttgattatt gctgacgatg tagatggtga
    12781 agcattgcca actcttgtat tgaacaaaat ccgtggaaca ttcaacgtag tagctgtaaa
    12841 agctcctggt ttcggcgatc gtcgtaaagc aatgcttgaa gatatcgcta ttttgacagg
    12901 tggtacagta attactgacg atctaggttt agaattgaaa gatgcaacaa tcgaaaactt
    12961 aggaaatgct tctaaagttg ttgtagataa agacaataca acgatcgtag aaggttctgg
    13021 agaaaaagaa gcaatcgaag ctcgtgtgca attgatcaaa aaccaaattg ctgaaacaac
    13081 ttctgatttt gaccgcgaaa aattacaaga acgcttagct aaattagcag gtggtgtagc
    13141 tgttgttaaa gttggtgcag ctactgaaac agaattgaaa gaattaaaat tacgcattga
    13201 agatgcattg aatgctacac gtgccgcagt agaagaagga atggtctcag gtggtggtac
    13261 agctctagtg aacgttatca gcaaagtttc tgctgtagaa gcagaaggag acgtggcaac
    13321 aggtatcaag atcgttgttc gtgctttaga agaaccaatt cgtcaaatcg ctgaaaacgc
    13381 tggttatgaa ggatcagtga ttgttgataa attgaagaac gtggaactag gcactggttt
    13441 caatgctgct actggagaat gggtaaacat ggttgaagca ggtatcgtag acccaactaa
    13501 agtaacacgt tctgccttgc aaaatgccgc ttccgtttct gccttgttat taacgacaga
    13561 agcagtcgtt gcagataaac cagaaccagc agcgccagca gctcctgcaa tggatccatc
    13621 aatgatgggc ggtatgatgt agtaattaag aaagaaccct attttaacag ggttctttct
    13681 tctttttttg ttttatttta agcaaaaggg gcaaagaagg ggcaaaaata tcaaaggcta
    13741 tttagtttat caattacttg ttgtttggct ctttttgtta catggttata aatagacaga
    13801 gttgtgtttg catcagaatg ccctacacgc tccataatgg ctttaagagg tacgcctaat
    13861 tcagataata gtgaaacgtg gctatgtctg aatatatgtg aggatagact tttttctaat
    13921 tccaactctt cctctacttt atggagtata gcgttaaatg agtgcagcgc aagcggagtg
    13981 ccacttgtag atataaatat atattgatcg ggatctgtgg gtctacctgc aagaatattg
    14041 tcagctatca cactttcaat caattctttt gcacgattgg gtaattgcac ttcacgttgc
    14101 gaataagtat tttttggagt tgtttttata gcattatcca ttttcacaga tgtgtaatct
    14161 aaggtcccat taatggaaat tttcccatcc tcatagtcct tcatttgcaa agctagcaat
    14221 tccccatatc tcaaaccagt taaatataga aactcagcta ttatgccgtg tagttttctg
    14281 cgaggattgg agtatagctg tttcagtatt tgatcaattt cttctttctc cagatatttt
    14341 ttattcatag aaagccttct tttttcttct tccacttttt taggatggat tttaactgct
    14401 agcgcagggt ttctttgaat gtattttcta tcgattgcat agtttagcat aacagataga
    14461 gttgtttttg tttgttttgt gtagttcagt gagaggtcac caaacgtata catatcttca
    14521 attatcttat taatgagtgt ctcatcaatg tttctaacga tcgtatcatc gcttatgtgc
    14581 ttagaaacat gtttcatcat cattggaacc ttcaaatagc tagtacgttt aacatgctgc
    14641 ttataatatt cataccattc tttatacagc tcaccaaaag tgatatctga tttattgtaa
    14701 tcttcgagtg cttctttaat ttttttatct agaattttct gagctttttt ccacgcttgt
    14761 ggtgaattac ttgtaagtgt tgtagatttt tttcgtgttt tttctgtata aggatctaca
    14821 tatctttcaa taaacttgaa ttggccatct ttggtctgtt caacccacac ttttaacatc
    14881 tcctatcatt tgctataata ggcataacaa atagacctat ataggtttgt tttctaaaag
    14941 cacgctctta ctctggacgg tggggcgtgt tttttattta ttatacagtt gctttaaaaa
    15001 tagctgcttt ttcaaatgat actaaaggag agaagtggat ttctatttct cccttttggt
    15061 ttaacgtgaa atgagcagtt acatccattt gttttcctgg agcaacagat cccattgtat
    15121 tttcgttggc atatgtctct gattttttgt catctggtcc atatacttcc acatctgtac
    15181 ctacagggat atctgaatcg ccatcatttt ttacggtata agtaattttt actacttgtg
    15241 caggttggtt ttcttcaaat tgatttcttt catcagttag ttctacacta tttagtgtat
    15301 attcagcatc accaactttt acagtgtcac caatcttata gaaggtatcg cttttttctt
    15361 ctttagaaga tgaagcagag gttgaagatt tagttacttt ttcgccacca ttatcacttg
    15421 ctttattact atctgatcca ccatttaaag cagaaccaat aattataatt aaaattactg
    15481 ctaataccca aaaccatact tttttgtaaa aaggtttact aactttatac atttttccgt
    15541 cttgacccat aacttttttt gccatttaaa tattcctcac ttcttgttat aatatatttg
    15601 cgatctcaga aatgaggtat gagtccgtgt gccagcacgg gcttttttaa tgttttgctg
    15661 aaatcggttt ttttaataat tcttgatgta ttcatacata ttgccttgcg taagaatatt
    15721 ttttcttaaa atagcattgg cagacagcat aacaagtgta tctgagctta ttctgaatag
    15781 aataatactc cataaatttt tcgagattaa actgagattc atcagtcagt tcgttctcaa
    15841 tatagatatt taataaaatt agaatagcta ttttatctgc ttctgtttca aattttgagt
    15901 gaaaagttgt agaagtatcg tacaaaactg aatattcaaa atgagaagcg gtgaaatgcg
    15961 caagttcatg agataaatga aaggcttctg cagtttcgcc atatagattt tcattcaaaa
    16021 aaatgattct aggcttcgga taatagaagc caggctcttt catttccatg taaactactt
    16081 tcaaattgta ttcgctcaac atttctttca atttcaaata catacaaccc atcactccaa
    16141 ctattcattt tcctctaaag ctttagcaat tgcaatcgct ttacgcattg tctccttaga
    16201 tatttctttt ccatcaaaag aaaaaacagt atcgttttct gataaatcca catgtttagg
    16261 ggtttctctt tcttctctac ctagaaggta gtctacagag actccaaagt aatcagctaa
    16321 ccgagctaac tcaatagaat taggagtttg tcttttccaa cttcctaaat atccattcga
    16381 atacccgaaa ttcatttcga gctgtctaat ggataatcct ttttcttttg ctaactcctt
    16441 tattatttcg taagtattca ttgataaatc aacctttctg aatgcttaca aaaaaagttt
    16501 agataaataa tgtaaaaata
//
LOCUS       DAZUKR010000046        16408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000046 DAZUKR010000000
VERSION     DAZUKR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.046"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACG89C_001415"
     CDS             complement(29..727)
                     /locus_tag="ACG89C_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS4899961.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACG89C_001416"
     CDS             complement(720..2072)
                     /locus_tag="ACG89C_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS4899962.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACG89C_001417"
     CDS             complement(2295..3695)
                     /locus_tag="ACG89C_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4899963.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACG89C_001418"
     CDS             3856..4593
                     /locus_tag="ACG89C_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS4899964.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACG89C_001419"
     CDS             4718..5197
                     /locus_tag="ACG89C_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS4899965.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACG89C_001420"
     CDS             5194..5961
                     /locus_tag="ACG89C_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS4899966.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACG89C_001421"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACG89C_001421"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4899967.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACG89C_001422"
     CDS             complement(6864..7466)
                     /locus_tag="ACG89C_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4899968.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACG89C_001423"
     CDS             7572..7802
                     /locus_tag="ACG89C_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899969.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACG89C_001424"
     CDS             7793..8041
                     /locus_tag="ACG89C_001424"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4899970.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACG89C_001425"
     CDS             8147..8731
                     /locus_tag="ACG89C_001425"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4899971.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACG89C_001426"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACG89C_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4899972.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACG89C_001427"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACG89C_001427"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4899973.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACG89C_001428"
     CDS             10632..10811
                     /locus_tag="ACG89C_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899974.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACG89C_001429"
     CDS             10833..11858
                     /locus_tag="ACG89C_001429"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4899975.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACG89C_001430"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACG89C_001430"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4899976.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACG89C_001431"
     CDS             13430..14377
                     /locus_tag="ACG89C_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS4899977.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACG89C_001432"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACG89C_001432"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4899978.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACG89C_001433"
     CDS             complement(15279..15521)
                     /locus_tag="ACG89C_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899979.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACG89C_001434"
     tRNA            15642..15714
                     /locus_tag="ACG89C_001434"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..>16408)
                     /locus_tag="ACG89C_001435"
     CDS             complement(15833..>16408)
                     /locus_tag="ACG89C_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS4899980.1"
                     /translation="VLGLLGLLFAPSIISIFSLTITGFIAFTLVFGKKDTKKMFSKPV
                     APVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEEL
                     FSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFN
                     QAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5596 a         2930 c         3309 g         4573 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtac
//
LOCUS       DAZUKR010000047        16371 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000047 DAZUKR010000000
VERSION     DAZUKR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.047"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACG89C_001436"
     CDS             complement(152..550)
                     /locus_tag="ACG89C_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS4899981.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACG89C_001437"
     CDS             complement(547..1320)
                     /locus_tag="ACG89C_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4899982.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACG89C_001438"
     CDS             complement(1317..2000)
                     /locus_tag="ACG89C_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4899983.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACG89C_001439"
     CDS             complement(2167..3054)
                     /locus_tag="ACG89C_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899984.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACG89C_001440"
     CDS             complement(3051..3920)
                     /locus_tag="ACG89C_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4899985.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACG89C_001441"
     CDS             complement(4057..5343)
                     /locus_tag="ACG89C_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4899986.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACG89C_001442"
     CDS             5616..6194
                     /locus_tag="ACG89C_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4899987.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACG89C_001443"
     CDS             6194..7123
                     /locus_tag="ACG89C_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4899988.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACG89C_001444"
     CDS             7120..8856
                     /locus_tag="ACG89C_001444"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4899989.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG89C_001445"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG89C_001445"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS4899990.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACG89C_001446"
     CDS             complement(9752..10261)
                     /locus_tag="ACG89C_001446"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS4899991.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG89C_001447"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG89C_001447"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4899992.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACG89C_001448"
     CDS             complement(11562..12653)
                     /locus_tag="ACG89C_001448"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS4899993.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG89C_001449"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG89C_001449"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4899994.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG89C_001450"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG89C_001450"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4899995.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG89C_001451"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG89C_001451"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS4899996.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4723 a         3620 c         2910 g         5118 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc a
//
LOCUS       DAZUKR010000048        16323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000048 DAZUKR010000000
VERSION     DAZUKR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.048"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..258)
                     /locus_tag="ACG89C_001452"
     CDS             complement(<1..258)
                     /locus_tag="ACG89C_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899997.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ACG89C_001453"
     CDS             complement(274..924)
                     /locus_tag="ACG89C_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4899998.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ACG89C_001454"
     CDS             complement(935..2107)
                     /locus_tag="ACG89C_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4899999.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ACG89C_001455"
     CDS             complement(2126..2998)
                     /locus_tag="ACG89C_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4900000.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ACG89C_001456"
     CDS             complement(3020..3940)
                     /locus_tag="ACG89C_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900001.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ACG89C_001457"
     CDS             complement(3940..4209)
                     /locus_tag="ACG89C_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900002.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ACG89C_001458"
     CDS             complement(4206..4586)
                     /locus_tag="ACG89C_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS4900003.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="ACG89C_001459"
     CDS             complement(5053..6516)
                     /locus_tag="ACG89C_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4900004.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="ACG89C_001460"
     CDS             complement(6580..9597)
                     /locus_tag="ACG89C_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4900005.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="ACG89C_001461"
     CDS             complement(9748..10587)
                     /locus_tag="ACG89C_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFS4900006.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="ACG89C_001462"
     CDS             10732..10977
                     /locus_tag="ACG89C_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900007.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="ACG89C_001463"
     CDS             11040..11462
                     /locus_tag="ACG89C_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900008.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="ACG89C_001464"
     CDS             complement(11556..11873)
                     /locus_tag="ACG89C_001464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900009.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="ACG89C_001465"
     CDS             complement(11900..12106)
                     /locus_tag="ACG89C_001465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900010.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="ACG89C_001466"
     CDS             complement(12087..12269)
                     /locus_tag="ACG89C_001466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900011.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="ACG89C_001467"
     CDS             complement(12283..12633)
                     /locus_tag="ACG89C_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900012.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="ACG89C_001468"
     CDS             complement(12659..12961)
                     /locus_tag="ACG89C_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900013.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="ACG89C_001469"
     CDS             complement(12964..13197)
                     /locus_tag="ACG89C_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900014.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14101..14631)
                     /locus_tag="ACG89C_001470"
     CDS             complement(14101..14631)
                     /locus_tag="ACG89C_001470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900015.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(14628..15743)
                     /locus_tag="ACG89C_001471"
     CDS             complement(14628..15743)
                     /locus_tag="ACG89C_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4900016.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="ACG89C_001472"
     CDS             complement(15749..16147)
                     /locus_tag="ACG89C_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4900017.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4320 a         3417 c         2558 g         6028 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atc
//
LOCUS       DAZUKR010000049        16209 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000049 DAZUKR010000000
VERSION     DAZUKR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.049"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1555
                     /locus_tag="ACG89C_001473"
     CDS             128..1555
                     /locus_tag="ACG89C_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900018.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1647..2012)
                     /locus_tag="ACG89C_001474"
     CDS             complement(1647..2012)
                     /locus_tag="ACG89C_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4900019.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2009..2374)
                     /locus_tag="ACG89C_001475"
     CDS             complement(2009..2374)
                     /locus_tag="ACG89C_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4900020.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2566..2814)
                     /locus_tag="ACG89C_001476"
     CDS             complement(2566..2814)
                     /locus_tag="ACG89C_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900021.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2813..2941
                     /locus_tag="ACG89C_001477"
     CDS             2813..2941
                     /locus_tag="ACG89C_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS4900022.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2998..4692
                     /locus_tag="ACG89C_001478"
     CDS             2998..4692
                     /locus_tag="ACG89C_001478"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS4900023.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4792..4965
                     /locus_tag="ACG89C_001479"
     CDS             4792..4965
                     /locus_tag="ACG89C_001479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900024.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5343..6476
                     /locus_tag="ACG89C_001480"
     CDS             5343..6476
                     /locus_tag="ACG89C_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS4900025.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6507..6827
                     /locus_tag="ACG89C_001481"
     CDS             6507..6827
                     /locus_tag="ACG89C_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS4900026.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6856..7320)
                     /locus_tag="ACG89C_001482"
     CDS             complement(6856..7320)
                     /locus_tag="ACG89C_001482"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900027.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7452..8705)
                     /locus_tag="ACG89C_001483"
     CDS             complement(7452..8705)
                     /locus_tag="ACG89C_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4900028.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8734..9051)
                     /locus_tag="ACG89C_001484"
     CDS             complement(8734..9051)
                     /locus_tag="ACG89C_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4900029.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9077..9397)
                     /locus_tag="ACG89C_001485"
     CDS             complement(9077..9397)
                     /locus_tag="ACG89C_001485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4900030.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9861..11276)
                     /locus_tag="ACG89C_001486"
     CDS             complement(9861..11276)
                     /locus_tag="ACG89C_001486"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4900031.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11293..12609)
                     /locus_tag="ACG89C_001487"
     CDS             complement(11293..12609)
                     /locus_tag="ACG89C_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4900032.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12759..13457)
                     /locus_tag="ACG89C_001488"
     CDS             complement(12759..13457)
                     /locus_tag="ACG89C_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4900033.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13746..14735)
                     /locus_tag="ACG89C_001489"
     CDS             complement(13746..14735)
                     /locus_tag="ACG89C_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4900034.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14815..15648)
                     /gene="msrA"
                     /locus_tag="ACG89C_001490"
     CDS             complement(14815..15648)
                     /gene="msrA"
                     /locus_tag="ACG89C_001490"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4900035.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15823..>16209)
                     /locus_tag="ACG89C_001491"
     CDS             complement(15823..>16209)
                     /locus_tag="ACG89C_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900036.1"
                     /translation="MDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSII
                     YRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYT
                     VTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5055 a         3267 c         2752 g         5135 t
ORIGIN      
        1 ataacataaa aaaccttttt taacggcatt tatatgcttt ttcagaaatt aactgatgat
       61 ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg
      121 ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg
      181 gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg
      241 caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc
      301 ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg
      361 caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt
      421 ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa
      481 agcttattca ttaaaaaaac aagtattagc ttactttaat gactgcggga tcgaaatcga
      541 ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta
      601 tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg
      661 tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat
      721 cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga
      781 ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg
      841 ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact
      901 attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca
      961 gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga
     1021 atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct
     1081 gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa
     1141 tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca
     1201 gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag
     1261 cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt
     1321 tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc
     1381 accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg
     1441 ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc
     1501 tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc
     1561 tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc
     1621 tcttgataca cgtcaggctc acaacctcac ttattttgga catctagggg tccgatctct
     1681 ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata
     1741 tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa
     1801 cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct tactcggtcg
     1861 ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca
     1921 ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat
     1981 ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt
     2041 tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg
     2101 caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga
     2161 ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac
     2221 gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa
     2281 caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc
     2341 attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt
     2401 gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta
     2461 atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat
     2521 aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag
     2581 cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc
     2641 atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga
     2701 cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa
     2761 taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa
     2821 aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg
     2881 aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta
     2941 gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg
     3001 agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat
     3061 ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac
     3121 ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat
     3181 ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc
     3241 tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact
     3301 gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg
     3361 atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt
     3421 attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc
     3481 actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg
     3541 tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg
     3601 catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt
     3661 tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc
     3721 aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat
     3781 gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt
     3841 gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga ttcagctact
     3901 ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc
     3961 tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat
     4021 cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat
     4081 gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc
     4141 acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa
     4201 ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca
     4261 agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta
     4321 gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa
     4381 gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc
     4441 gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc
     4501 aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa
     4561 agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt
     4621 cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa
     4681 atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc
     4741 aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct
     4801 tttgaacacc gtttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca
     4861 gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa
     4921 gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga
     4981 aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac
     5041 tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt
     5101 ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg
     5161 attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat
     5221 agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa
     5281 atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc
     5341 caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc
     5401 caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct
     5461 ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac
     5521 acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc
     5581 aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca
     5641 tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg
     5701 gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag
     5761 actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat
     5821 cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata
     5881 cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg
     5941 ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta
     6001 attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt
     6061 atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag
     6121 agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat
     6181 tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata
     6241 tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc
     6301 atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac
     6361 tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat
     6421 atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc
     6481 agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg
     6541 aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa
     6601 atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg
     6661 gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg
     6721 gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg
     6781 acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat
     6841 ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact
     6901 ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc
     6961 tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa
     7021 tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt
     7081 tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat
     7141 ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc
     7201 agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt
     7261 cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa
     7321 tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat
     7381 tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt
     7441 tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg
     7501 acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct
     7561 gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata
     7621 acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc
     7681 attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac
     7741 atcgttggtt cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata
     7801 tatggtttgt ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct
     7861 gttcctccta agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta
     7921 tgtccagctg cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc
     7981 aaaggatcga ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat
     8041 gcaagcagta aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact
     8101 gtatagaaag aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa
     8161 gcaaacgcag agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc
     8221 ggtggcacac catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag
     8281 atttctgttg aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct
     8341 aatgctccgc ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct
     8401 tttcggtagc cgttagctaa atgataagca agcaagaaag caatcaagac agaaagaatg
     8461 gcaaatgtac cgttccaagc agcagccccg aatcctttcc atgattcacc accaaaaatt
     8521 gagttcatca attcttggaa cccgggaatc ggtaagttat tgatcattac tgctaatgct
     8581 cctaagatca ttaacggcat actcaccata aatgagtccc gaatcgcaac taaatgccgt
     8641 tgtgcaccaa tttttgatgc tacaggtata aaatgtttct ccataaatgc aacaaattta
     8701 tccataaatc ttccctcttt tctttactgt agattacgcg ttcaatcgac gatacagatt
     8761 aatgatctct tttgctaaat ctgtaaaagc tatagatgtc atcagatgat cttgactgtg
     8821 gaccgtcaaa agcgtgactt caacgtgatt tccttgtgct tcttgtgtca gcatctctgt
     8881 ttgagagtga tgtgcttgga ctaaagattc ctctgcatct ttgatttttt catctgctaa
     8941 agtgaaatca ccttttttcg cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc
     9001 gccaaacatg atcagaccca tgatggcttc tagatttttt tgatcttcca tttattgtat
     9061 cctccgataa tttttattat ttgtttatta gtttttctgc ttggtcgagt actttttcac
     9121 cattcatcat gccatagtca gccatattga ttacatctaa aggaatgcct ttaggtgcta
     9181 gcttttcagc aaattgattt tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta
     9241 catttttaga ttctagttga ttatccgcat ctgaagcaga tacagcaaag atgtctgctt
     9301 ccattccttt tgcttctgct gccttttgca tttttgtgac taggagactt gtactcattc
     9361 cagctgaaca tacaagcatg attgttttct tttccatttt aaacacgttc ctttctatac
     9421 ttacacttgt atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat
     9481 catcacattt aaggtctata ccaagttgca tacacactaa aaaagaaagc gcacactaaa
     9541 cacacaaatt aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac
     9601 aaaacttgtc acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata
     9661 agtccaacca agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat
     9721 gcaggacagc tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat
     9781 tagctcaaaa cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa
     9841 catctacaaa aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta
     9901 tcaatcaaag tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc
     9961 aattcgattc gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca
    10021 gtgaatgccc ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag
    10081 taatgcaaat gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca
    10141 tccatatact gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata
    10201 ttatgataat tttcttttag atacatggct atatcataca tgattttcgg atagatttcc
    10261 catcccctgg aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca
    10321 ggatggaaag gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa
    10381 tattgattga ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt
    10441 gcaggatcga aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat
    10501 ccttttacca tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct
    10561 ttttggtctt ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca
    10621 atccttccac cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact
    10681 ttatgataat tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca
    10741 tccaaatagc aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc
    10801 ctatcaccgt aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt
    10861 cttgaagacc agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa
    10921 caaatcatcg gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga
    10981 gcatattctt catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata
    11041 gaagtccgat aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt
    11101 tccataaaat tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt
    11161 ttataccagc tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt
    11221 tgccaagcag aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt
    11281 tcctcctgtt ttttagatcc catcattttc cacacgattt gctactttta caaatggcag
    11341 atagatcaaa atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc
    11401 tgctgttgcc agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc
    11461 tttcgccgca aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt
    11521 taagataaac ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg
    11581 ttcattgatg ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc
    11641 ccgttttcca actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat
    11701 actcatatac atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc
    11761 aaaagcttcc gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac
    11821 catttgattt ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa
    11881 aatacccggt aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat
    11941 atcataaagg tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga
    12001 tacagtcaga atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt
    12061 atcaggcatt ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga
    12121 aaggatggag accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa
    12181 tacgttttct actataacag tctgattttc attcactaat tcataaacag ttggattaac
    12241 agataaataa gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt
    12301 ggctaattcg atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata
    12361 gctgatggca gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc
    12421 taagccattt tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat
    12481 aattaccgga atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt
    12541 gattctcgtt gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga
    12601 tcccctcatt cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt
    12661 acatttcgat gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc
    12721 atctgatgta tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct
    12781 attattttct gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca
    12841 gcaagtgcat tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg
    12901 attttcagat gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt
    12961 gggataatat gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg
    13021 atgtctaatt gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt
    13081 aattttttca tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc
    13141 ttaggctcca ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca
    13201 atcaaagaaa aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc
    13261 gctccttgcc gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc
    13321 gtgcttcgcc ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct
    13381 acttgcaact gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg
    13441 gttttatttt cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat
    13501 tataacacgt ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata
    13561 gacatacaaa cgaagaagat atttaccata aaaaagacta ggataaaatt cactcctagc
    13621 cttcgatcat ttttattttg aacgacaaaa aatatgatgt tcaataacag acgatcttca
    13681 ttcgattctt gatgtcatct tttgtttttt ttaatatatg gataaaaagt ttgattacat
    13741 cgtttctatt ctagaatttg gtcgattctc aattcttctt ctggtgagaa atctaaatta
    13801 tccatcattc gaacactttc ttgtaactga cttaaccgac ttgctccgac cagtacactt
    13861 gtaacagatt tttgtcgcaa attccatgca agggccattt gcgctaatga ttgtcctcgt
    13921 gatacggcga ttgtttgcag tgctttgatt ttttccagag ttgatccaac ttgttcttct
    13981 gaaaggaatg gtatttcctt acgatgtgct ctagaatctt caggaatacc atgtagataa
    14041 cgatttgtca gcaacccttg agcaagcgga ctgaacgtga ttgcccctag cccttcttct
    14101 tctaatactg gagatagatc gtcttctagt agtgcacgag aaaacatatt gtagcgcatc
    14161 tgatgaataa tgaacggtgc ttcctttctt tttaaaattt cagtcatttt ttttgtatct
    14221 tctccattat agttggatat accaatatat aatgcttttc cttgtcttac aagttgcatc
    14281 aaagcttctg ctgtttcttc caaaggtgta tcagggtctg ggcgatgatg atagaagatg
    14341 tcaacataat ctagccccat tctttggaga ctttggtcac aacttgaaat gatggattta
    14401 cgagagcccc attccccata tggcccttcc cacatatgat aacctgcttt gctagaaata
    14461 atcagttcat cacggtaggc ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct
    14521 gcagctccag cagggcctcc ataattattc gcaaggtcga aatgggtaat tcccatgtcg
    14581 aatgcgcctc ttaaaatctc tctttgatta tggataggat cataatcacc aaaattttgc
    14641 cataatccga gagataaaat cggtaatttc aaacctgaat gaccggattt acgatattcc
    14701 attttttcat aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt
    14761 ttaaaacgct ttcttttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc
    14821 aaaatgattt tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa
    14881 ttgattacgt gcttgctgga tttttttata acgtttcggt tgttttttgt aaaattgctg
    14941 atgatagttt tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg
    15001 gtggaaagtt cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg
    15061 ttcgacttga taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa
    15121 tgtatctgtg ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc
    15181 tcttgtgtca taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg
    15241 atcataattt ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc
    15301 ttttttcgat tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg
    15361 ttcttgatag tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc
    15421 aaatccttcc gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat
    15481 agctaatgga cgtagtgttg cttctagttt tgataactgt tctttaacgg atatattttc
    15541 tcttatgtca ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt
    15601 caaaatcaga ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc
    15661 tttccgaaga agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataag
    15721 cgattacttc atccccctac agatttttcg tataaaaaag agagaatcct tatcgacaga
    15781 ttctcctttt taatcaattt attttataaa tgaaaagtta atttaccttt tatggaaaac
    15841 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
    15901 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
    15961 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
    16021 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
    16081 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
    16141 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa
    16201 ataatccat
//
LOCUS       DAZUKR010000050        16147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000050 DAZUKR010000000
VERSION     DAZUKR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.050"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..467
                     /locus_tag="ACG89C_001492"
     CDS             153..467
                     /locus_tag="ACG89C_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900037.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(901..1267)
                     /gene="ssrA"
                     /locus_tag="ACG89C_001493"
     tmRNA           complement(901..1267)
                     /gene="ssrA"
                     /locus_tag="ACG89C_001493"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1438..2211)
                     /locus_tag="ACG89C_001494"
     CDS             complement(1438..2211)
                     /locus_tag="ACG89C_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4900038.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2355..2705
                     /locus_tag="ACG89C_001495"
     CDS             2355..2705
                     /locus_tag="ACG89C_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4900039.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2686..3402
                     /locus_tag="ACG89C_001496"
     CDS             2686..3402
                     /locus_tag="ACG89C_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4900040.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3402..4850
                     /locus_tag="ACG89C_001497"
     CDS             3402..4850
                     /locus_tag="ACG89C_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4900041.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4920..5429)
                     /locus_tag="ACG89C_001498"
     CDS             complement(4920..5429)
                     /locus_tag="ACG89C_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900042.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5419..6144)
                     /locus_tag="ACG89C_001499"
     CDS             complement(5419..6144)
                     /locus_tag="ACG89C_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900043.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6367..8487)
                     /locus_tag="ACG89C_001500"
     CDS             complement(6367..8487)
                     /locus_tag="ACG89C_001500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4900044.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8717..9895
                     /locus_tag="ACG89C_001501"
     CDS             8717..9895
                     /locus_tag="ACG89C_001501"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS4900045.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9911..10669
                     /gene="aroD"
                     /locus_tag="ACG89C_001502"
     CDS             9911..10669
                     /gene="aroD"
                     /locus_tag="ACG89C_001502"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4900046.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10692..11177
                     /gene="ybaK"
                     /locus_tag="ACG89C_001503"
     CDS             10692..11177
                     /gene="ybaK"
                     /locus_tag="ACG89C_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS4900047.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11248..12501)
                     /locus_tag="ACG89C_001504"
     CDS             complement(11248..12501)
                     /locus_tag="ACG89C_001504"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4900048.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12619..13968
                     /gene="gdhA"
                     /locus_tag="ACG89C_001505"
     CDS             12619..13968
                     /gene="gdhA"
                     /locus_tag="ACG89C_001505"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4900049.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14080..15426
                     /locus_tag="ACG89C_001506"
     CDS             14080..15426
                     /locus_tag="ACG89C_001506"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4900050.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15734..16120)
                     /locus_tag="ACG89C_001507"
     CDS             complement(15734..16120)
                     /locus_tag="ACG89C_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4900051.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5274 a         2847 c         3232 g         4794 t
ORIGIN      
        1 agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt
       61 tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt
      121 gggtatttaa atagttgcta ggaggatagc ttttggattt ttacagtaat tttatcttaa
      181 taatagcaat attattactt ctaaatattt ggttttttga taagagtaga aatgcaggga
      241 taggttttag aaccaaacga agtacgtctt ctgaaaaaaa gtgggtatat tcacagacta
      301 ttttttacgg aggggttatt tcaatcagtt tactttcttc aacactttat tcttttaatg
      361 ttatagacgt ttccatgtca aattttatat ctattattgg aatccttatt tctgcaatta
      421 ttacacaact acttcttgtg tttgaagaga agtcaaaaaa taattaaagc aattatgcct
      481 tttagtacag gaattattac ttcttacaaa tgtatagcga atgtgaagcc tgctgtttta
      541 attgttctag aaaaactttt gagtgaacaa ttattttatg ttaggatgaa ttaatttctc
      601 tagagtttat aattaaaaaa cttatttgaa ggaacatgct taaaccataa ttgtgttttt
      661 ccttccatca gcataaaagt tcacctcaaa aagagtgtac gaacaaaagt tctttttgta
      721 aaccttaaaa tagattttcc tgaatacgta ttggggattt ctaaatgttt gaatgttttt
      781 ttgaatgcaa aatcaatgca aaaaattttg tatttctcta cttttctctc cttgaccaaa
      841 ataaaaaaac gttgatttaa caacgttttt catacatgtt tatctgtctc aaccatacat
      901 atatggagac ggcgggagtc gaacccgcgt ccaaacacat cgccacttaa aaatctacgt
      961 tcatagtcaa ctcatttaag gattcacttt acagcttgcc gagtgacagg cattctgtat
     1021 cgctagtctg atgttctctt tttcaactta cagacggaaa gttgaaacgt atcccactaa
     1081 agttaggacc cttactcgag cacatgggcg atgccgagag gatctacgct aactgttttt
     1141 aggcagctaa agcgtaagag ttgttttcgt ttttagcagt tatatttaac tgtaacgttt
     1201 ttacgtagac gtaacctacg aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa
     1261 cgtccccgtt agctaacaag ggttacgtgt atttttcata caaaattagt atagcatata
     1321 ttgggataaa ttcaataaaa aagcgaatca tatcccatta tttatataat aactgcccct
     1381 ttcttaagaa aaaataagcg attttcttaa gaaaagaggt agcttcaaaa aaagatctta
     1441 aatcagatca aaatacttca atgcgtggac aattccgcct tctgtatttt tctttgtgat
     1501 aaaggtagaa agttctttta atccgtcata ggcgttgccc atagcgattt tgttgtcgca
     1561 agcttccaac aaggcaaaat cattcggacc atcgccaaat ccaaaagtag gaactttttc
     1621 cagctgaagt tctttttgca gacgtttgac acctgcccct tttgagattc ctttcttcac
     1681 tgtatcgatc gagtaaggac cattccgata aaaagttagc tcaggaaaat gttcgtgata
     1741 gtattcgtca cctgattgag ataagatcag caacatattt actgtgtttt ctttaaagcc
     1801 taaaggatca atttccggaa catctgaatg aatgaagtcg taagcattga tcattgtttg
     1861 gctatgaccg gtacaccaga ttttctgatc gttatagtaa gaaagttcat gaccgttcaa
     1921 ctgtacatga tcatacattt tttgacattc ttctgaagaa aatgtttgag aatagatttc
     1981 ttcgccgtct actcgaatga aagaaccatt catggtgacc gcagaagtaa tgcccgcatc
     2041 ttccatgata gagtggattt ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt
     2101 gttttctttt aatgcgttca tagcttttgc cacttcgggt gtgatttttg atttcgcatc
     2161 tagtaatgtt ccatcaagat caaagaaagt gattgcacga taatttgaca caaataaaac
     2221 tcctttttta ttggaataag tttatcttaa caaagaatga acaaaagaca aacctttcgt
     2281 gaagaattcc tgaacatatt ggttcagtca acaatttaga gtatactata agaagagaat
     2341 ttaggaggga aagaatgatt tttttcagta ttggtatctt attcttaata attggcagtc
     2401 tttttcggat tctgccctca aaaggaaatc tgccttttta tgggtatcat tcgcctcttg
     2461 ccgcaaaaac agacgctcat tggcggctgg ctcaaaagac aagcggaaac tggttttttc
     2521 ttatgggctt actgatggca ttgatcggct attatttaaa gacatctggg catacgaatt
     2581 attttctgat tgaaatgctt ctgctagtat ttccaattat gcctattttt attatgacag
     2641 aaaagaaatt acagaaatat gacctggaaa ctggagggaa cgataatgaa tattttaatg
     2701 attgaagata atcaatctgt ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg
     2761 gaagcaactt tcaagtatga tggaaaagaa gggctcgatg cctttttaga aaacccacaa
     2821 cattgggata tgattacact agacctgaat ttgcccacac tggacggtat gctagtagga
     2881 agagagatcc gcaaagtttc aagtactgtg ccgattatca tgctgacagc tagagattct
     2941 gaaagcgatc aggtgatcgg cttagagatg ggggcagatg attatgtcac aaaacctttc
     3001 agtcctctga cgcttattgc tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa
     3061 gcaagagaag aagcagcagt ttcagatgat cattttgata tcgagactag tcatttcaag
     3121 atgaatacga aaacacgaga agcgtatctg gacaaccggc caattgaagg actgacgcct
     3181 aaagagttcg atctattgtt tactttagca aaaaaaccac gtcaagtatt ctctcgggag
     3241 cagttgcttg aattagtctg ggattatcag tactttggtg atgaacggac agtggacgcc
     3301 catatcaaga aattacgaca aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt
     3361 tggggagtcg gctacaaatt cgatgattct ggtgttgcct aatgcgttat ttgtatcagc
     3421 aactcttagc tttttgcgga atggtcgcgt tgatcttatt gatcgttgga gtttctttta
     3481 cacaactgac aaaacaaacg attgaagaaa acaattacca gcaactgttt ggatatgctg
     3541 aatcagttga aaagacaacg caaacctatg ccgatacgct cccgcagttt agtcaagatc
     3601 aggcgtttca aaatgcattg ctgctgacag agcaagtact gacagaacaa aatgtgaact
     3661 ttatatttat cgataaaaac gaacgtgtca tttatccgac taatacagct ttactgaatt
     3721 ttaccttaac agctgatcaa tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat
     3781 cgaataaaaa tattttagga caagcacagg cgacctctta cgcacttgtg ccatttaatt
     3841 tgaatcatga attctatgga gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca
     3901 gcgttcgtcc aattacactg aacctgttca aaggatttat tttttccagt atcgttgcag
     3961 ttattgccag ctatgctttt gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg
     4021 cgacgaaaga agtgacgaat ggtaattttg atgtacagct ccccgtacat gacaaagatg
     4081 aatttgatga gttagcagat gatttcaata aaatgacgaa ttcattgaaa gaatctcaag
     4141 cagaaattga agaacaagaa aatcgtcgaa gacaatttat ggcagatgct tctcacgaga
     4201 tgagaacacc tcttacgaca atcaacggac ttctggaagg gttagaatac aatgcaatcc
     4261 ctgaaaatca gcgagaaaac gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc
     4321 gtttggtgaa tgaaaacttg gattatgaaa aaatccgtac gaatcaaatc agtatggtca
     4381 ttaaaaaatt caatggaaca gaaacattga aaaatattgt tgcacagtta gaagctaaag
     4441 cagaagcagc aggagataca ttgacattga aagccgttga tgatattgat gtttacgctg
     4501 attatgaccg atttgtccaa atcatggtca atattattca aaacgcgata cagtttactg
     4561 aaaacggcca aatcacgatt actttagaaa aaggctatct tgaaacgatc atcacgattg
     4621 aagatacggg aattggaatg tctgaacaac aaatgaaaag tatctgggat cgttattata
     4681 aagtagatcc ttcaagaaaa aatacaaaat atggtgaatc aggattaggt ttaccaatcg
     4741 tgcagcaact tgtacgcttg cataaaggaa aattagaagt agaaagcgaa ctaggaaaag
     4801 gaacaaaatt cgctgttcgt ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg
     4861 gtataaaaga tcatgaatcg atcttctata ccgcttgttt ttactataaa atgattgatt
     4921 tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta
     4981 aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct
     5041 tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg
     5101 gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc
     5161 gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt
     5221 ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac
     5281 atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt
     5341 ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt
     5401 tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg
     5461 gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga gcccaataag
     5521 agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt
     5581 aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg
     5641 gatatctggc agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc
     5701 ataaactgac tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg
     5761 aaagaacagc tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggcg
     5821 ccaattgatc aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag
     5881 gaaggcaaat agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc
     5941 taaagccgga agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac
     6001 ttgattggct aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc
     6061 agaaaagctt gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc
     6121 agtagcatag cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata
     6181 tgaataaagt atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta
     6241 aaaagcaaga ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa
     6301 cactgtttat tcgaaaagag gagcagcgac aataattttg tcgctgctcc ttttaaattt
     6361 attttgtcat tcggaagaac ttgtggtcgt tccgtgaagt tcttggtacg tttgcgtttt
     6421 ataaagatca acagttgatt gtccgttgtg ttcactaaag acactagttg acttgtcacc
     6481 aagagatttt tcagcttctt ttaatttctc taagccattc ttgtagtcat aatcactggt
     6541 atcaattggg tctaatccac tattcgtata gaaccgaaga agatcaccat tattgatttg
     6601 gtcagaaaca gctaactgtt tatcaacttt ttctttcagt gaatcaattt cattctgtac
     6661 ttcctcagta ggttcagtaa tagcttcacc agtttcgtta ctatagatcg taccaccgta
     6721 gtaagtatat tttggtgtaa caaaatcacc atcacggaaa gaaacaagct gttcatgatc
     6781 tttcgagaat agatcttgtc ctaactgaat ataatttttt gtatctacac ctagtaagtg
     6841 aagtaatgta ggcaaggcat caacttgtcc gccgtaagtg tgattgattc cgccctgtgt
     6901 ttgtccaggg atatggatca tataagggac acgctgcatt tgtgcattgt cgtagtttgt
     6961 ccaagtagaa cttgtttttc caactaattc agctagattt ttatttcttg aattcgagac
     7021 accgtaatgg tcaccgtaaa ggacgatgac tgaattatca tacaagccgc tttctttcaa
     7081 ataattgaag aattcttgta cggcggtatc taaataattg gctgtcgcaa agtaaccgtt
     7141 gatcgtttca tcatttgtat tggctaacgg gaatcctgct tcgtcattgg tgaattttga
     7201 ataaggataa tggttagata cagcaatgaa ttttgaataa aatggttgtt gcagacgttc
     7261 caagtattgt acggattgtt ggaaaaatgg tttatcatgc aacccgtatt ggaacgaatt
     7321 gtcatcatta acatcataat aagaagaatc aaagaaataa tcatatccta aattcttgta
     7381 tgtctcgttt cgattccaga atgtaccagc gttcccatgg aataccgcac tagtgtaacc
     7441 ttgtgtttga cttaaaatat caggtgcagc ttggaaagta ttttttccgc ctaattgtgt
     7501 gaacaaggag ccttgattca agccaaacaa tgaattttcc atcagcgttt ccgcatcgct
     7561 tgtttttcct gcttttactt gatggaaaaa gttgtcaaaa ctgaacgtgc tgttgctgtg
     7621 gtaaagacta ttgatgaaag gtgtaacttc atgctggaga ccattctcat ctgtcaattt
     7681 ataatcgatc aggaattgct ggaagctttc taaatggata taaatcacat tttttccttt
     7741 agcgagcccg aacaaatcgt catttggtgc tgcataatgt tcttttacat aattttctac
     7801 ttctttcata tcattgggac ttgcttctgc ccgcacttgg tttgtctggt aagtttgaac
     7861 accgtcataa accatgaaag cattcaagcc aagatatttg accaaataat cacgagagaa
     7921 tgttcgagtc agcaattctg gacgatcagc ttccgccata aaaagatttc ctgagaaaat
     7981 cataactgct aatgtagaga tcgcaaaagc cattcttgct cggatcgggc gagggtccaa
     8041 cttgattttt tttgcaatca gcagaacagt caagataata aaatcgatcc aatagatcac
     8101 atcatatggg cgaaacagtc gaatggcact ttctcccaga ccactcgcta ctttcccagc
     8161 acctaaaatc gtgttgatcg taatgaagtc cgtgaattct cgataataag tgacattcga
     8221 aaaaacaagc aatgtcaaaa gaaaataaat gaccatcatc gtgatataag caactctagg
     8281 gcgtctgata taaagagcaa ttccaagtaa taacaatgta gttgcaatag ggttgataaa
     8341 caagataaag tattgaatgc cactttcgat tccgagatgg aaatcaacta aataggcaaa
     8401 aatcgttttc aaccaaagta aaacgactag taaagaaaaa aatccgagcc tcttattcaa
     8461 aaaggcaggt gtttttatac atttcaagat aatcttcctt tctgcacttc ttaatcacag
     8521 aagtgtcatg aaaccatgtt acacagctca cttattctca tttattttac tagtatgaca
     8581 atatagtgtc aattgaaacc agaagtttat gctgtttcat taaggttttc tttaagcttc
     8641 atgaaaaaat gagcagtaag acaagtccta tgttttttgt tatacttggg aagagaaagc
     8701 taaagcaggt gaaggaatga aagtaaaagt aaaaaaaaga gcaagcaatc gattgaaaaa
     8761 aggatatcca ctgatccaaa aagaagacct tgctcatccc caaaaagaaa ccacgcaatg
     8821 ggtggaatta gttgattcgc aaaaccaatt tatcgcaaaa ggatatctag gaaagcagaa
     8881 taagggaatc ggttgggtat tgagccagca gaacatccca tttgatcaat ctttttttga
     8941 agaatttttt gtgaagtcaa aagaaaaaag aactttcttt ttttcagatg attctactac
     9001 cgcttttcgt atattcaatg gagaaggaga cggaatcggc ggtctgattg ttgattatta
     9061 tgcaggttat gctgtttttt cttggtataa cgaaacgtta tatgcacatc gtgaattatt
     9121 gattcaagct tttcgtgaaa tctacccgga aatcgtagga ggctatgaga aaattcgatt
     9181 tgaatcagta aatctgcctg agagccagtt catttacgga aaagaggcac cggaaccctt
     9241 gatcgtaaaa gaaaacggag taaaatttgc cgtttattta aatgaaggat taatgactgg
     9301 gatcttttta gaccaaaaag aagtacgggg atcattagca gaaggtttcg cttctgggaa
     9361 aacagttttg aacatgttta gttataccgg cgccttttct gttgctgccg caatgggagg
     9421 ggcaatcgaa acaacaagtg tcgatctagc aaaacgcagt ttaaaaaaaa cacaggaaca
     9481 gtttgaagtg aatggtttgg atgtcgatca gcaaaaaatc attgttatgg acgtattcga
     9541 gtatttcaaa tacgccaaaa gaaaacaatt tttatatgac atgattgtat tggatccacc
     9601 aagttttgcc agaaataaga aaaaagtctt tcgcgtagca aaagattatg gggaactggt
     9661 caaggatagt ctggatattt tagcagaaga cgggatactt attgcgtcta caaatgctgc
     9721 gaatgtctcg atagaacaat tccaaacaat gattgaagag gaattccatg ccaaaaatgt
     9781 ttcttttgaa caacttgctt tatatcgtct gccaaaagat ttccagacga cggatacctt
     9841 tttagaaggc aattatctga aagtgtttgt ttatcaaatt aaaaataacc attagaagga
     9901 gcgatgcaga atggtttggg taaagggatt gaatatagga agcggtagac caaagatttg
     9961 tgcgcctctt atcggggccg tccgagagga agtattacgt gaagcagttc ttgcacgtga
    10021 agcaggggcc gatttagtcg aatggcgagt cgatcattat cgtgaagttt tttcttttga
    10081 tgaagtagca gaaacattgg ttcttatcca tgagatgctt gatggactgc cgttattatt
    10141 tacgtttcgt accttagctg aaggcggaga acaagacata tcgatccgtc agtatcgcga
    10201 cctttatgaa catgtgatac acacaggatt agtagatatg gtcgatatcg agttgttcaa
    10261 agtagaaagt ctaggaaaaa gactgataga ggaaatcaag tctttaaaca ttcctttaat
    10321 tatcagtagt catgatttta aagaaacacc agcagaccct gtcttgcttt accgattgaa
    10381 tgttatggaa cattttggag ccgatatcgg aaaactggca gtaactccca ataatgaacg
    10441 agatgttttg cggttgatgg agttgactag acgggcaaat gcttttgtct ccatgcctat
    10501 tattacgatg tccatgggaa atcttggtaa aatttctcgt ctggctggcg gaacgacggg
    10561 ttccgttatg acatttggtt ccttaagcag cgattctact tctgctccag gacaattacc
    10621 ggtcaaagca ttgaagcaaa ttattcaatt attaaatgaa ccgttgtaga acaagtttga
    10681 ggtgatattt tttggcaaag aaaaaaatag taaaaacgaa tgcaatccgt atggtcgaac
    10741 aaaaaaagat tccctataca gagcacgaat atgagtggga cgaaagccac ctaagtgcgt
    10801 catcggtcgc tgaacagctt ccagaaagcc aatctcgtat atttaaaaca ctggttgctg
    10861 tgggcaatgt gacggggcct cttgttgctg tgataccggg agaagccgaa ttgaatctaa
    10921 aaaaattagc aaaagtttca gggaataaaa aagtagaaat gctccattta aaagatttgg
    10981 aagcaacgac cggatacata cgtggtggct gctcgcctat aggtatgaaa aaactatttc
    11041 ctacttattt agatcaaatt gcagaaagct atgaacaaat cattgtctcg gctggcagaa
    11101 gagggctaca gatggagtta gcacctcaag atatctgtgc attaacgagc ggacaatttg
    11161 cagatataaa acaataaatc atacgtataa aagaaagaag gaggccggga tgttgtttgt
    11221 ccagtctcct tctttctttt ttatacatta tgctctttgc ccggcattca ttcctgctac
    11281 atgccctgta gcgaaagccg ctgtgatatt aaagccgcca gtataaccat tgacatccag
    11341 taattctccc gcaaaataca gaccatcaat gattttactc tccaatgttt taggataaat
    11401 ttcctttaga gaaacccctc cgccagttac aaatgatttc tcaataggaa aagttttgtt
    11461 gattgatagc ttaaactctt tgcatagttg aacaaaatct tgaatttgtt tttgagaggc
    11521 ttgttgtcct gtcagatgat ccatttcaag ccgctcaaga aagaaaacta acaatcgctc
    11581 aggcaatata ccatgccaag cattgaccaa gttctttttg gttgctttac ttttttctgt
    11641 caatatatga ataagctctt gtttggtttg tgtagggaaa caatccagag aaagtgtcac
    11701 tggttctccc gttttctcta attctttatt gacaaaactt gaacaacgca gtgcagccgg
    11761 accagaaata ccgaaatggg tgaaaagtaa atccatcgta tgttcagtca atacacgacc
    11821 ttttttattt aaaacgcgta aagtgatgtc ttgtaaagaa agcccttgaa gtgttttctc
    11881 ctgaatatac ggttcatccg atatcaaggg ggactctgtc gcataaagag gcgtcaccgt
    11941 gtgacccact cgcttcacga tcttgtaccc atctccagta gaaccagtag atggataagt
    12001 ccgtccacca gttgttaaga taatggcagg agcatggaac tcttcgaatt ccgtgcgtat
    12061 accgtaaatt ttatgttctt tgtggatcaa tttctctact cgagtagaga atagaagcgt
    12121 cacatctagt tctttcagac gattgagcaa tgcttcgata atcgtttttg atttatttgt
    12181 tacaggaaac atccgtccat gatcctcttc ttttaaatgt acgccttgtg attcaaagaa
    12241 gttcatgata tcgaaattat tccattgcgc aaacgtacta tataaaaact ttccatttcc
    12301 aggaatatgt gcaatcaaat catcaacagg acgattgttc gtaacattgc accgaccacc
    12361 acctgtcatc agtagttttt ttcctgcttt tttgtttttt tcgatcaaga gtacgcgtgc
    12421 gccttgttca gctgcactga tcgctgccat catcccgctt gttccagcgc cgactacgat
    12481 cacatcatac agtttttcca ttccctcacc tcaaggaaag tgtagcatat ctgaagaaag
    12541 aaaacagaaa aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga gggaagataa
    12601 gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat tcaagaaaag
    12661 ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga attcatgccc
    12721 acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat cttagaactt
    12781 ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga tgaaggaaac
    12841 tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc ttataaaggt
    12901 ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc ttttgaacag
    12961 attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg aagtgacttc
    13021 gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt catgctggaa
    13081 ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg tgtaggagct
    13141 agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga tactggtgtg
    13201 ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc tactggttat
    13261 ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt taatcaaaaa
    13321 acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa agtgcaagag
    13381 ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga tccagaagga
    13441 atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt gacggaatac
    13501 gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac attgaaagaa
    13561 aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag cttggccgaa
    13621 atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc atgtacagta
    13681 aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat
    13741 gctggaggag tagcagtatc tgctttagaa atgagtcaga acgtccagcg attgacttgg
    13801 gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta ccatacgtgt
    13861 cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct
    13921 ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa aatgaaactt
    13981 tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa
    14041 agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat tcattttgac
    14101 tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca aagccaagta
    14161 acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt ccgcggctgg
    14221 atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa
    14281 aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc ttatttaggc
    14341 gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga tagcaacaca
    14401 agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata tattgctgac
    14461 ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca
    14521 actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga
    14581 caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg tgcagttaaa
    14641 gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat cggcggacgt
    14701 ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc agatatcgat
    14761 gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa actagaagaa
    14821 aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg caaagtaact
    14881 gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg gaaacaattg
    14941 tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc aaacttctca
    15001 actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat ttttgaaaca
    15061 gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc agaagattta
    15121 gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa agcatttgaa
    15181 ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa aattccagaa
    15241 atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt aggtatttca
    15301 ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta caagaaaaac
    15361 atgttcgcac ttctaggcaa accaggcttt gaagatcttg ctaaagaatt gaacgaacgc
    15421 ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca tgttcgcttt
    15481 aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta ttcagaataa
    15541 ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga ttgattgcct
    15601 tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa ggttaattct
    15661 gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg cttttttgcg
    15721 atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt accttatcgc
    15781 tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc aagatcgttc
    15841 cttcggggta agcagaacca ttggcagagt caccagaagt aatgacatcg aaatctaact
    15901 gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct cttccaatta
    15961 cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg actaccgcat
    16021 aataatcttt acctacataa gtagactgat agtagtccca acctttccat cctataaaag
    16081 caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc cttttttatt
    16141 tttatta
//
LOCUS       DAZUKR010000051        16081 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000051 DAZUKR010000000
VERSION     DAZUKR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.051"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..984)
                     /locus_tag="ACG89C_001508"
     CDS             complement(163..984)
                     /locus_tag="ACG89C_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4900052.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1081..1359)
                     /locus_tag="ACG89C_001509"
     CDS             complement(1081..1359)
                     /locus_tag="ACG89C_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900053.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1627..3636
                     /gene="metG"
                     /locus_tag="ACG89C_001510"
     CDS             1627..3636
                     /gene="metG"
                     /locus_tag="ACG89C_001510"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4900054.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(4115..4702)
                     /locus_tag="ACG89C_001511"
     CDS             complement(4115..4702)
                     /locus_tag="ACG89C_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900055.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            4877..6199
                     /locus_tag="ACG89C_001512"
     CDS             4877..6199
                     /locus_tag="ACG89C_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4900056.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            6354..7130
                     /locus_tag="ACG89C_001513"
     CDS             6354..7130
                     /locus_tag="ACG89C_001513"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4900057.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            7123..7692
                     /gene="rnmV"
                     /locus_tag="ACG89C_001514"
     CDS             7123..7692
                     /gene="rnmV"
                     /locus_tag="ACG89C_001514"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS4900058.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            7685..8569
                     /gene="rsmA"
                     /locus_tag="ACG89C_001515"
     CDS             7685..8569
                     /gene="rsmA"
                     /locus_tag="ACG89C_001515"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4900059.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            9020..9649
                     /locus_tag="ACG89C_001516"
     CDS             9020..9649
                     /locus_tag="ACG89C_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900060.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(9773..10882)
                     /locus_tag="ACG89C_001517"
     CDS             complement(9773..10882)
                     /locus_tag="ACG89C_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900061.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            11091..11513
                     /gene="mgsA"
                     /locus_tag="ACG89C_001518"
     CDS             11091..11513
                     /gene="mgsA"
                     /locus_tag="ACG89C_001518"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4900062.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      11700..11798
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            11836..12372
                     /locus_tag="ACG89C_001519"
     CDS             11836..12372
                     /locus_tag="ACG89C_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4900063.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(12412..14175)
                     /locus_tag="ACG89C_001520"
     CDS             complement(12412..14175)
                     /locus_tag="ACG89C_001520"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900064.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(14178..15893)
                     /locus_tag="ACG89C_001521"
     CDS             complement(14178..15893)
                     /locus_tag="ACG89C_001521"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900065.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         5112 a         2950 c         3234 g         4785 t
ORIGIN      
        1 caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt attcagaaag
       61 tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg
      121 ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat ggtgccccgg
      181 cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac cgatctcatc
      241 gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat aatcgatccc
      301 taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg
      361 tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt tcaagatgat
      421 ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga ctaaatcaag
      481 cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa
      541 ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa taaaggctaa
      601 gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga atgtatgcag
      661 ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt ctcttaagac
      721 gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc atgaatttcg
      781 ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt ctttgttata
      841 gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac gttcctgttt
      901 ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata atttttcttc
      961 ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag tactttttac
     1021 cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct aggttatcat
     1081 ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca gataaaacgg
     1141 caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga tatatacgtc
     1201 aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc catggaggtt
     1261 aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta atgaaagaaa
     1321 agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag tcccttacta
     1381 agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg ttttttagta
     1441 gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt aaaggataga
     1501 ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa gctaacagca
     1561 tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata aaggaggaag
     1621 agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta tccaagcggc
     1681 aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc tcgttataaa
     1741 cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg tcaaaaaatc
     1801 gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa aatggcggct
     1861 gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat tcgcacgaca
     1921 gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga acagggagat
     1981 atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt ctttacagaa
     2041 acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg taaagcgcca
     2101 agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat gagcaaatac
     2161 gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc agaatctcgt
     2221 aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc agtttcacga
     2281 acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt gtatgtatgg
     2341 atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga tgaaagtcta
     2401 ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt tcgctttcat
     2461 acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa aaaagtattt
     2521 gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt
     2581 tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta tttgcttcgt
     2641 gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc ccgtgtcaat
     2701 tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat gatcaacaaa
     2761 tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga tagtgaactg
     2821 tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa aatggaattc
     2881 aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata cattgatgaa
     2941 acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg
     3001 atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat catgacagaa
     3061 acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa tcttgaagga
     3121 ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac accaatcttc
     3181 ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc tgaaggtact
     3241 caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa
     3301 gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc agaagtcatc
     3361 aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga tgctggagac
     3421 agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc aagcgaattg
     3481 atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc
     3541 agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt aatcgaagca
     3601 ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg tatgacacta
     3661 gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat tccttcttat
     3721 tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa tcctagaaaa
     3781 agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg aatcgtaaaa
     3841 acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca tctggtactc
     3901 ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac atctatattt
     3961 atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg ttcaattagt
     4021 ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa acaatcatcg gctttgactt
     4081 cgatgattgt tttctttatt ttgtaaaaat cctatcactt gattttatgg taacctttta
     4141 tttttgtctt caaacggctt ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga
     4201 cataccagaa aatcgtacgt ccgataccat aatgttgata gagctttgtc aacatcacgt
     4261 gtaacaagat caaccctaaa agcaaggagc cagctataaa ccaatgatta tcccaatgca
     4321 tgacttcact aagtgccatc actccaaaag gatagaaata aaaatagatc ccgactaagg
     4381 tcatccgcca gttctttgta aatgttccga tattcaataa aaagaaaatc gctgaaagca
     4441 aaataccgat aggcggaaaa agcccaacca aaaaggaata caacatcccc cacaagatca
     4501 tcacaggacc tttgatcaat gcaacgattg cgatgaacaa agtcagcaat attagttttt
     4561 gtgataacgt tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa tgccaatggg
     4621 actgctgttg ttctttacgg gaaaaatcga cccggttgga taaataatct gctaatttcg
     4681 gataagaacg cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta ggtttagtat
     4741 acgaaaaata aaaaagaaat acatccgtct taagagggag tttttcactg aaagtggaga
     4801 aaaaagaatg gtaaattaaa atattctcag ttatactgaa aattatacca aatttcaaag
     4861 aagaaagagg atgggaatga aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt
     4921 agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac
     4981 tgctagtact cagtctacaa gtttgacatt gctagcttgg cttttgggtg gtttcttaac
     5041 gatctgtgct ggattgacag tggctgaatt agctacagca atcccggaaa ctggtggagc
     5101 tgtaaaatat attgaagctg cgtatggaaa attgcccagc tttttattag gatgggcgca
     5161 aagcttgatt tactttcctg ctaatattgc tgcattatcg attatctttg cgacacaatt
     5221 gacgaactta ttacaattat ctaccgatta tcttttgttg attgcagtga tcaccgctgt
     5281 ttctgtgaca gggttgaatt tgttagggac aaaagtcggt gcttctgtac aatcagtgac
     5341 gttgattgtt aaattaatac caattgctgt gattgtcata tggggattat tgaccccagg
     5401 acaaggaact gtccagttat ttccaatcga agctggaaaa gatgttactt ttgtcgaagg
     5461 attgagcagt gcgttgttag caacgttgtt tgcttatgac ggctggttag gtgtcggtgc
     5521 aatggcaggg gaaatgaaac ggccggaaaa agatttgcct aaagcaatta ttcttggttt
     5581 gagttttgta acagtcgttt atctattgat caattttgtt tttttgaaaa ctttgccgat
     5641 tgatcatctt gccggtaatt tgaatgcagc ttctgaagca tcagacgtca tttttggagg
     5701 aatcggtgga aagctggtca ctattgggat cttgatctcg gtatacggtg cattgaacgg
     5761 ttatacacta actgggatcc gcgtgcctta tgcgatggct ttagaagacg agctaccttt
     5821 cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt
     5881 ccagttagca gtagcttgta ttatgatgag ccttggttct ttcgactttt taacagacat
     5941 gttgattttt gtgatgtggc tattcagttt actgattttt atcggtgtct ttattttaag
     6001 aaaaaaagcg ccggaattgc cacgtccata taaagtacca ctttatccaa tcgttccgat
     6061 tattgcgatt ctcggtgcta ttttcatttt aggtatgacg atgatgacgc aaacgaaatt
     6121 agcattgatc ggtataggtg ttaccttgat aggtatccca gtctattatc aaaaaaagaa
     6181 aaaacgctca gaagagtaaa atatttcaac aacagacaga aaaagtacga actaggtcct
     6241 ctagatcttc gtgctttttc tatttcaata aaacaagttc ttctattttg atttttgcta
     6301 cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa aggagagata aacatgatat
     6361 ttgattctca tacccattta aatgctgaac agttcaatga agatattcca gaaacgattc
     6421 agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga
     6481 ttgaaaagtc tttgcaatta agccatgact atccaaatat atacagtatt atcggctggc
     6541 atccgacaga ggctggcagt tatacgaaag aaatcgaaaa taaattacaa gaacaattga
     6601 ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga ttatcattgg atggaagacc
     6661 cgaaagaagt ccaagataaa gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc
     6721 taccaatcag tattcatact agagaagcac ttgaagacac gtatcaaatc ctaaaagaag
     6781 aagatgttcg agatatcggt ggaatcatgc atagtttcag tggtgattat gaatgggcaa
     6841 aacgatttct tgacttaggg atgcatattt catttagcgg tgtcgtcact ttcaaaaaag
     6901 cattagacgt ccaagaggct gcagcaaatg taccaatcga acgattactt gtggaaacag
     6961 atgcccctta tctggcacca gttcctttta gaggaaagcg aaatgaacca ggctatactc
     7021 gttatacagt agaaaagatt gctgaacttc ggaatcttcc gacagaagaa gtggctgccc
     7081 aaacatggac aaatgcgcac cgattatttg gtctaagcga acatggataa gatgaagatc
     7141 caagagatta tagtcgttga aggaaaagac gatacaagaa ggctgcggga agtagttgat
     7201 gcagatacga tagaaacaat agggtctgca atcaatgaag agatcctgat gcaaattgaa
     7261 catgcccaag agactcgtgg agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa
     7321 ataagaaaaa tcattatgga agctgttcct caggcaaaac atgctttttt gccgcgcaca
     7381 caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt
     7441 ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt ttctgaaatc
     7501 actagacaag accttttaga ctatggttta atagctgggg cacaagcaaa agaaaaacga
     7561 gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt
     7621 ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa
     7681 caacatgagt gaatatcgtg aaattgcgac accctctaga acaaaagaga ttttattgaa
     7741 acatggtttt tcatttaaaa aaagtttagg acaaaatttt ctgactgaac caaatatttt
     7801 aaggaaaatc gtagaaactg cgcaaataga tgaacatacg aacgtgatcg aagttggacc
     7861 aggaatcggt gcattgacgg aacaattagc caagcatgca aaacaagtcg tagcatttga
     7921 gatcgatgat cgtttgatcc ctgtattggc tgatacgatg caaccctatg acaatgtgac
     7981 tattgtacat caagatattt taaagaccga tttgtcaaca gctgttagag aaacatttca
     8041 tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac tatattacga caccgatcat
     8101 gatgcatttt cttgaatcag atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga
     8161 agtagctgat cgtatctctg ctgaaccagg aacgaaagct tatggttcat tatcgattgc
     8221 tgttcaatat tatatggaag ccagtttagc attcatcgta ccaaaaacag tatttgtccc
     8281 tcagccaaat gtagattcag cgattttgaa actgacaaga agagatatac cggcagtcga
     8341 agtgacagac gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa
     8401 aacgctttgg aataacttgc agcattcgta tggcaaagat gaccagacaa aagcatggtt
     8461 agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt
     8521 acaagaattc gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt
     8581 gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     8641 agttcctcat gtttttcgtt tgtttgggct tgttaccgca gatgcaagct tttgaacacc
     8701 gtttaagaaa gaggttgtgg gcctgccgtt taactctaag tcacaaggaa caatttcaaa
     8761 aaacagcttc tcatgttttt gagttagttg gtcttattac ttgatgcaag cttttgacca
     8821 ctattatgcc gaacaaggag atgtgacaca aattttgtca catctcctat ttttgtttct
     8881 tgatcaaaaa attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat
     8941 atgtgtcttg acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac
     9001 atattaggag agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt
     9061 attgattggg atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc
     9121 ttcctttact ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt
     9181 tgcagtggct gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat
     9241 cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa
     9301 gcatccgagt atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg
     9361 gttcaatttt gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt
     9421 ttactttatt ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt
     9481 catcttgatg gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt
     9541 tatagcagga acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga
     9601 aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct
     9661 caaaaaaaaa actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt
     9721 cctaaaaaga gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa
     9781 gtttgatgac ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa
     9841 ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag
     9901 tagaaccagt ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt
     9961 ctgatttcac tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt
    10021 ctggacgaat accgaagata acttcttttc cttcataacc tttttcaact aatactttgt
    10081 tttttccttc aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca
    10141 atgtcacatt aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga
    10201 cgggtgtatc ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga
    10261 taacgattcg gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg
    10321 ttgtttcaag acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag
    10381 catctaaatt tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc
    10441 gtcccaatgc cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt
    10501 attctgtcag acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat
    10561 catatttacg taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca
    10621 atgcgtagtt ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga
    10681 cttttccgcc gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca
    10741 aagtagttga ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga
    10801 ttgatagatt aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt
    10861 ttttcagagc catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta
    10921 tttaaagtat aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc
    10981 ttttttttgt caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg
    11041 atacactgga gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg
    11101 ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa
    11161 caatattgga aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag
    11221 caaccggctt gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg
    11281 gagccatgat ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg
    11341 cacagcccca tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc
    11401 cgttagctac gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc
    11461 tagattttcg aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa
    11521 gcgtatggaa aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact
    11581 gataatatgc gatataatac gaacgtattg gataatataa acaataatat tagtttttcg
    11641 tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat
    11701 acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag
    11761 gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta
    11821 aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc
    11881 ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt
    11941 aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg
    12001 tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca
    12061 ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc
    12121 tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc
    12181 atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc
    12241 tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt
    12301 gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc
    12361 cacgctaaat aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc
    12421 tgtgaattgt gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg
    12481 ggtgcccatt tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat
    12541 tgtggacagg cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc
    12601 ttgttggatt tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa
    12661 cataggggga ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga
    12721 tatctttaac ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa
    12781 ttcatacata taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt
    12841 cccaaaacga agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat
    12901 tccaaaactt ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt
    12961 aattgttcct tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc
    13021 ggcccctgtt tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc
    13081 ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc
    13141 tttgatattt ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg
    13201 atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat
    13261 tccagaaagt tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat
    13321 cacaccaaca gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat
    13381 cgataaataa gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa
    13441 ttgtgcccgt tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact
    13501 agcttcttct tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa
    13561 gcccgagata tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc
    13621 aacgatccaa ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag
    13681 tggacttaga cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa
    13741 ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact
    13801 gacgatattt ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc
    13861 aaatgcatct tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg
    13921 gatgagccat tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg
    13981 gaacagtcga ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat
    14041 ttgtaacgtc atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca
    14101 aatgtctttt gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa
    14161 tttttttact ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct
    14221 gattgagcaa gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact
    14281 aagatatgat gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg
    14341 ccaagataag tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt
    14401 aaagagtcat ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt
    14461 cgctgttttt gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca
    14521 tttggaagct gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct
    14581 ttttctgtgg cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt
    14641 acagaagttt ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga
    14701 acatccattc cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat
    14761 tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc
    14821 ttcagctgaa atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca
    14881 acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct
    14941 tcaagaatcg aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca
    15001 cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata
    15061 taattgatca aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg
    15121 ccaactgtca gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat
    15181 agggcagaga tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg
    15241 aacttctctg tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga
    15301 ttctgactaa cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga
    15361 acggtttttt tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa
    15421 aacagaccta cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac
    15481 ggtgctctga tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg
    15541 tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa
    15601 gtattgattt ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact
    15661 gaagcatagt attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc
    15721 cataagccca tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag
    15781 gaagccatca ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg
    15841 cccaaaatga tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc
    15901 ctcctattat ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga
    15961 aatttagaat gaaaagagaa caagcctttt cataaatgaa agttttatta tgaagaagct
    16021 ttagaaattg aatagactat tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc
    16081 t
//
LOCUS       DAZUKR010000052        15982 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000052 DAZUKR010000000
VERSION     DAZUKR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.052"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG89C_001522"
     CDS             226..894
                     /locus_tag="ACG89C_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4900066.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACG89C_001523"
     CDS             complement(1094..1480)
                     /locus_tag="ACG89C_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900067.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACG89C_001524"
     CDS             complement(1452..2834)
                     /locus_tag="ACG89C_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4900068.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACG89C_001525"
     CDS             complement(2990..4177)
                     /locus_tag="ACG89C_001525"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4900069.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACG89C_001526"
     CDS             complement(4184..5215)
                     /locus_tag="ACG89C_001526"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4900070.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACG89C_001527"
     CDS             complement(5231..6685)
                     /locus_tag="ACG89C_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS4900071.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACG89C_001528"
     CDS             6860..7765
                     /locus_tag="ACG89C_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS4900072.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACG89C_001529"
     CDS             7856..8533
                     /locus_tag="ACG89C_001529"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4900073.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACG89C_001530"
     CDS             8530..8700
                     /locus_tag="ACG89C_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900074.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACG89C_001531"
     CDS             8814..9710
                     /locus_tag="ACG89C_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4900075.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACG89C_001532"
     CDS             complement(9765..11135)
                     /locus_tag="ACG89C_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4900076.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACG89C_001533"
     CDS             11373..12119
                     /locus_tag="ACG89C_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4900077.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACG89C_001534"
     CDS             12116..12955
                     /locus_tag="ACG89C_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4900078.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACG89C_001535"
     CDS             12956..13906
                     /locus_tag="ACG89C_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4900079.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACG89C_001536"
     CDS             14047..14799
                     /locus_tag="ACG89C_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900080.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ACG89C_001537"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ACG89C_001537"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4900081.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5187 a         3001 c         3220 g         4574 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta ac
//
LOCUS       DAZUKR010000053        15850 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000053 DAZUKR010000000
VERSION     DAZUKR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.053"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..751
                     /locus_tag="ACG89C_001538"
     CDS             68..751
                     /locus_tag="ACG89C_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS4900082.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="ACG89C_001539"
     CDS             753..1589
                     /locus_tag="ACG89C_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4900083.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="ACG89C_001540"
     CDS             1600..3354
                     /locus_tag="ACG89C_001540"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900084.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="ACG89C_001541"
     CDS             complement(3630..3971)
                     /locus_tag="ACG89C_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4900085.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="ACG89C_001542"
     CDS             complement(4039..6210)
                     /locus_tag="ACG89C_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4900086.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="ACG89C_001543"
     CDS             6421..7278
                     /locus_tag="ACG89C_001543"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4900087.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="ACG89C_001544"
     CDS             complement(7349..8206)
                     /locus_tag="ACG89C_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4900088.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="ACG89C_001545"
     CDS             complement(8191..10893)
                     /locus_tag="ACG89C_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4900089.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="ACG89C_001546"
     CDS             complement(10906..12195)
                     /locus_tag="ACG89C_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS4900090.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="ACG89C_001547"
     CDS             12345..13391
                     /locus_tag="ACG89C_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4900091.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="ACG89C_001548"
     CDS             13393..15546
                     /locus_tag="ACG89C_001548"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS4900092.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4504 a         3304 c         2927 g         5115 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg
//
LOCUS       DAZUKR010000054        15841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000054 DAZUKR010000000
VERSION     DAZUKR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.054"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..1529
                     /locus_tag="ACG89C_001549"
     CDS             126..1529
                     /locus_tag="ACG89C_001549"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4900093.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            1529..2962
                     /locus_tag="ACG89C_001550"
     CDS             1529..2962
                     /locus_tag="ACG89C_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4900094.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            3107..4033
                     /gene="trxB"
                     /locus_tag="ACG89C_001551"
     CDS             3107..4033
                     /gene="trxB"
                     /locus_tag="ACG89C_001551"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4900095.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            4247..5974
                     /locus_tag="ACG89C_001552"
     CDS             4247..5974
                     /locus_tag="ACG89C_001552"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS4900096.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            5989..6339
                     /locus_tag="ACG89C_001553"
     CDS             5989..6339
                     /locus_tag="ACG89C_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4900097.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(6431..7291)
                     /locus_tag="ACG89C_001554"
     CDS             complement(6431..7291)
                     /locus_tag="ACG89C_001554"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS4900098.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            7383..7811
                     /locus_tag="ACG89C_001555"
     CDS             7383..7811
                     /locus_tag="ACG89C_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4900099.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            7973..8149
                     /gene="rpsU"
                     /locus_tag="ACG89C_001556"
     CDS             7973..8149
                     /gene="rpsU"
                     /locus_tag="ACG89C_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4900100.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            8176..8622
                     /locus_tag="ACG89C_001557"
     CDS             8176..8622
                     /locus_tag="ACG89C_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS4900101.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(8776..10155)
                     /gene="brnQ"
                     /locus_tag="ACG89C_001558"
     CDS             complement(8776..10155)
                     /gene="brnQ"
                     /locus_tag="ACG89C_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4900102.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            10861..11832
                     /locus_tag="ACG89C_001559"
     CDS             10861..11832
                     /locus_tag="ACG89C_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4900103.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            11856..14048
                     /locus_tag="ACG89C_001560"
     CDS             11856..14048
                     /locus_tag="ACG89C_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS4900104.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            14065..14541
                     /gene="ybeY"
                     /locus_tag="ACG89C_001561"
     CDS             14065..14541
                     /gene="ybeY"
                     /locus_tag="ACG89C_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4900105.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            14525..14920
                     /locus_tag="ACG89C_001562"
     CDS             14525..14920
                     /locus_tag="ACG89C_001562"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4900106.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            14935..15834
                     /gene="era"
                     /locus_tag="ACG89C_001563"
     CDS             14935..15834
                     /gene="era"
                     /locus_tag="ACG89C_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4900107.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
BASE COUNT         5295 a         2778 c         3442 g         4326 t
ORIGIN      
        1 tatttacaaa aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga
       61 tttctattgt ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg
      121 agaacatggc aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg
      181 agttgatgga aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg
      241 tagatgtaga agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt
      301 gggatgcttc tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga
      361 aagatgcgga ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag
      421 acgagcggat ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga
      481 tattcaaagc ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt
      541 tatgtccaaa tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag
      601 ttatcagata tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga
      661 tgatgacaga accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg
      721 gtctaaatca tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag
      781 aattgattga tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc
      841 cgtttttcaa agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt
      901 actatcgtga agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa
      961 gagcgcagca agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat
     1021 tagaccataa acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct
     1081 gtgaaacgat tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga
     1141 atgatggagc tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg
     1201 gcgcacaagg agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt
     1261 tgcaagtaat gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg
     1321 ggacagcttt acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac
     1381 ggatcatgga tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa
     1441 tcagccgatt aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag
     1501 gtaatacaca tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg
     1561 tgctacagca gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa
     1621 cgtagatgtt gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat
     1681 gtttgaattt gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg
     1741 ttataaagaa gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat
     1801 cgcctggagt cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa
     1861 attttatgag gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat
     1921 tacgcatttt gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga
     1981 actcgttggt ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt
     2041 caaatattgg ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc
     2101 agggctgtat tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca
     2161 tgagttggtt gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa
     2221 agtaggctgt atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt
     2281 ttgggcttct agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg
     2341 agaatatccc gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga
     2401 accaggggat gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta
     2461 tgcttcccga gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt
     2521 tgcttctgtc aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt
     2581 aggcttgaga ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt
     2641 agaaaacggc ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta
     2701 ccgaatcgat tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg
     2761 agtcgatcta ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg
     2821 tgagatgaag aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac
     2881 attagcccgt tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg
     2941 agaaaatctt tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt
     3001 tttcgagtat aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat
     3061 tggctgtaaa gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat
     3121 cgttattggc gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt
     3181 atccgtattg atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat
     3241 cgaaaattat cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga
     3301 aagtgtctca agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca
     3361 cggcagccat aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat
     3421 cgcaacagga tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg
     3481 aggcgtttct tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt
     3541 catcgggggt ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga
     3601 agtagtgatc gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc
     3661 atttgcaaat gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa
     3721 cgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt
     3781 agcagcagac ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga
     3841 agcaggtttg acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat
     3901 gccaggcgtt tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc
     3961 tgttggtgac ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga
     4021 aacaacagca taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt
     4081 ttttcgcgtg aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa
     4141 agaatccttt cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa
     4201 tgctatactt attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca
     4261 agtgtatgaa caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa
     4321 tgatctgggt gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg
     4381 tactgcaggt atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt
     4441 aagacaagca acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg
     4501 ccgaggtgtc gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc
     4561 agccaaaaca ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac
     4621 accagagctt tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc
     4681 atctcacaac ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat
     4741 gcctcctgca gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa
     4801 agtcgaagta ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga
     4861 agaagtggac gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt
     4921 tgaaacaatg gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat
     4981 gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca
     5041 agctgtggca gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc
     5101 ttttgaatat gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga
     5161 tcctgatgca gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt
     5221 gactgggaac caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag
     5281 aggcacttta ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac
     5341 agcaattgcg gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat
     5401 tgctgaaaaa atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga
     5461 agaaagttat ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt
     5521 gcttttatta gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg
     5581 cttgcaaacg atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat
     5641 gagcggacaa gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc
     5701 accgacagaa tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag
     5761 aacagaccgt gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta
     5821 tacattggaa gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa
     5881 gttttatatt ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta
     5941 tgaagcgatt atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg
     6001 gtaaacttta gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa
     6061 aaatcaaaag tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt
     6121 aaaattctgc tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg
     6181 gaaatggagc agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc
     6241 aagtctcatc gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat
     6301 attttgaacc agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa
     6361 cactggagtg tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag
     6421 acaaaaatga ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta
     6481 ggtctaaagc attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga
     6541 tcactacaca tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt
     6601 tttcgatatc ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac
     6661 ggatattatt taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt
     6721 cccacagctg tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat
     6781 ttttatttgc taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa
     6841 cgacatctgc ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt
     6901 ccattgcttt gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc
     6961 gaattggctt gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga
     7021 aactaaataa ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga
     7081 ttaatgtatg caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg
     7141 ttttttcatc tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag
     7201 ccatagtagc ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa
     7261 aagtaacgat ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat
     7321 gtttattata ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag
     7381 tcatgaaaaa acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta
     7441 aatatactaa aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat
     7501 ctgctaaaga agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga
     7561 tttaccggaa tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg
     7621 gtgaaatgaa gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta
     7681 tctgtacagt gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag
     7741 aacagctagg tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa
     7801 attgtcaata aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc
     7861 cttgacggtc aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata
     7921 tatttcaata acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa
     7981 aacagtcgtt cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt
     8041 gtcaaaagcg ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt
     8101 aagacgcaag aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga
     8161 attggagttg gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg
     8221 aaagcgaaag ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac
     8281 gagcagatta aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc
     8341 gaaatgaaac agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta
     8401 gctgaaaaag taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc
     8461 gacgaagaaa ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg
     8521 aaagaatttg gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga
     8581 aaccaagtga atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc
     8641 ggacagtgat tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa
     8701 gaaagaataa gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa
     8761 ttgtgtttga tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag
     8821 gcgataggcg taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca
     8881 gccaagtcct aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac
     8941 gacagtttgc tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac
     9001 ggcagaaaag aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt
     9061 taaggaaagc aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga
     9121 ccattggatg atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa
     9181 ccgcagccat gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga
     9241 aacaacgagt cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct
     9301 agcaccgaag ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc
     9361 tgataaaggc atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat
     9421 gacttctaac gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc
     9481 tttaaaacca agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac
     9541 tgcatcaacc gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga
     9601 atctgcagca gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat
     9661 agctaataat gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt
     9721 tggatgtaca gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc
     9781 taatgaatca tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc
     9841 tgtccgcggt gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc
     9901 agcataccat tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac
     9961 tacagctaag atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc
    10021 gctgatccag tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga
    10081 cccgaagaac atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt
    10141 ttttgtttct tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg
    10201 ttgcagaaac tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat
    10261 aaaaaaaagc ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt
    10321 tttcgcttaa aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct
    10381 gataatggag gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt
    10441 ctattcgatt tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag
    10501 cctactgttt tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt
    10561 tttttttgaa atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa
    10621 tacccagaga atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt
    10681 acatgatcag ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta
    10741 acgtttcttg gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata
    10801 gtttcttctg agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt
    10861 ttgacggaag agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg
    10921 cttttaggtg cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc
    10981 catacacgtg gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc
    11041 tcagtcattc ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat
    11101 gtcgtgacgg cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat
    11161 gaggaagaga tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa
    11221 aagaaatatg tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg
    11281 acaggaaaaa cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg
    11341 caaaaaatca ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc
    11401 ggggatctaa aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa
    11461 atttttggat tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct
    11521 ttagcttaca tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag
    11581 aacacgacag ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg
    11641 attgtcaatg gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt
    11701 catgcagaac gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt
    11761 gacgttgttc ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc
    11821 catcaagaat aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac
    11881 tacaagaaaa acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta
    11941 tctgcggcgt tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag
    12001 tagcagaaga aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa
    12061 aaagaaaact ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag
    12121 atgaccagaa caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca
    12181 tcaacaaatc ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc
    12241 ctactacaga agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa
    12301 atgttgcgtt ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg
    12361 aaactgagat ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca
    12421 aacaagttcg tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat
    12481 atttgaatgt cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg
    12541 tagtgaatga cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag
    12601 ctgtacaacc agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg
    12661 atgcttcagc tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt
    12721 ttccgctaat tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt
    12781 cacttcaatt ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta
    12841 tgtcagtcag catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt
    12901 atatcccgct gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc
    12961 gaagagcagg gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg
    13021 attcgattgg cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca
    13081 tggcaacgat tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg
    13141 tcattatttt tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg
    13201 gtgatgcaac gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct
    13261 tgctgacgat cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg
    13321 tattaaatga actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc
    13381 caggtactta tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa
    13441 tcggcggacg ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca
    13501 aacatgctag ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct
    13561 tattgccaga agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat
    13621 tggaagatta taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca
    13681 cgctaatgcg ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc
    13741 aattccgtta tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg
    13801 acagttgtga ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt
    13861 ttgtccataa tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga
    13921 ccttgaaaga aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc
    13981 attctcggat caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag
    14041 aagaatagaa agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc
    14101 tcagaagaga aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag
    14161 ctgccagagg aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc
    14221 aatcgtgatt accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa
    14281 gaaggcgaag acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta
    14341 ctaggagaca tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat
    14401 tcatatgatc gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat
    14461 gaccatatga caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat
    14521 gcctatggac tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga
    14581 atttgctgtc caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt
    14641 tgcgttcggt atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg
    14701 gttatggctg cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt
    14761 tgaaaatgta gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa
    14821 agatatggca gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat
    14881 tttatttgtc cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca
    14941 gaacataaat cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta
    15001 ttgaaccgaa tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga
    15061 aataaaatcc aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca
    15121 ggaatccata aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg
    15181 ttgaaagaag tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga
    15241 gacgatttta ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat
    15301 aaaatcgata agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag
    15361 atgccttttg cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta
    15421 atggatgtat tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg
    15481 actgatcatc ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta
    15541 acacgagacg aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag
    15601 aatgataaaa tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc
    15661 attatcggca aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc
    15721 gaaaatcttt taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg
    15781 cgagacaaaa aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct
    15841 g
//
LOCUS       DAZUKR010000055        15811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000055 DAZUKR010000000
VERSION     DAZUKR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.055"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..791
                     /locus_tag="ACG89C_001564"
     CDS             39..791
                     /locus_tag="ACG89C_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4900108.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            788..1702
                     /gene="pfkB"
                     /locus_tag="ACG89C_001565"
     CDS             788..1702
                     /gene="pfkB"
                     /locus_tag="ACG89C_001565"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4900109.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1720..3651
                     /locus_tag="ACG89C_001566"
     CDS             1720..3651
                     /locus_tag="ACG89C_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4900110.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3762..5321)
                     /locus_tag="ACG89C_001567"
     CDS             complement(3762..5321)
                     /locus_tag="ACG89C_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS4900111.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5476..7710
                     /gene="pcrA"
                     /locus_tag="ACG89C_001568"
     CDS             5476..7710
                     /gene="pcrA"
                     /locus_tag="ACG89C_001568"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS4900112.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7727..9763
                     /gene="ligA"
                     /locus_tag="ACG89C_001569"
     CDS             7727..9763
                     /gene="ligA"
                     /locus_tag="ACG89C_001569"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4900113.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9869..10174
                     /gene="gatC"
                     /locus_tag="ACG89C_001570"
     CDS             9869..10174
                     /gene="gatC"
                     /locus_tag="ACG89C_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS4900114.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10174..11646
                     /gene="gatA"
                     /locus_tag="ACG89C_001571"
     CDS             10174..11646
                     /gene="gatA"
                     /locus_tag="ACG89C_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS4900115.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11643..13073
                     /gene="gatB"
                     /locus_tag="ACG89C_001572"
     CDS             11643..13073
                     /gene="gatB"
                     /locus_tag="ACG89C_001572"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS4900116.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13083..14138
                     /locus_tag="ACG89C_001573"
     CDS             13083..14138
                     /locus_tag="ACG89C_001573"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4900117.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14249..15622
                     /gene="rlmD"
                     /locus_tag="ACG89C_001574"
     CDS             14249..15622
                     /gene="rlmD"
                     /locus_tag="ACG89C_001574"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4900118.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15625..15753
                     /locus_tag="ACG89C_001575"
     CDS             15625..15753
                     /locus_tag="ACG89C_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4900119.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5378 a         2900 c         3474 g         4059 t
ORIGIN      
        1 aaaaatatgc tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgcagac
       61 aaaaaatcct agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc
      121 ttttgaatgc ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt
      181 ttctagaaag agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata
      241 tgagtgaaaa atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg
      301 aaaccattaa tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga
      361 ttccttttct gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa
      421 atttagttga tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta
      481 atgctgtttt aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat
      541 ttatgggaat gaacggtgcc catcttgaac aaggattcac gactccggat ccagaagagg
      601 cagctgttaa acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata
      661 ccaaattcaa ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa
      721 ccgagcgttg tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg
      781 caatacaatg atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga
      841 tgggttaaaa ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa
      901 agggatcaat gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt
      961 tttaggaggg ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc
     1021 tttgttcaca accgtaaaag aagatacaag aatcaatatc aaactaaaat cagacactga
     1081 aactgagatc aatggcttag gcccatctat ttcagatgaa gaaatcgatg agttgaagca
     1141 agcattagga acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccagctag
     1201 cctgcgaaaa ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt
     1261 cgtgattgat acaacaggtg atgacctaaa aaatgcactt gttaagaaac cgttgttgat
     1321 caaaccaaac aatcacgaat tagctgagct ttacaacaca accttccaat ctgtagaaga
     1381 aatcattccg tatgggaaaa aattactagc tgaaggggca cagaatgtgc tgatctcaat
     1441 ggctggagac ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt
     1501 tcgcccattg aaaaattcag taggtgcagg ggattcaatg attgctggct ttattggggc
     1561 ttacagccgt acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc
     1621 aacagctttt tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt
     1681 tgaaatcaca aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg
     1741 gtcaaagaag cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa
     1801 atggtccaaa aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga
     1861 attctagccc gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac
     1921 gcgaaaaaca gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta
     1981 gattatgaag ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa
     2041 ggcgcaaacg acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca
     2101 gatttcgtag gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa
     2161 acagctgaag agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa
     2221 gcttctgctc atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc
     2281 gctcacactt acatggcaga agatgctttg aagaaaaaag cgaaagaaat gggtgtggag
     2341 atcaaagtcg aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc
     2401 gcccgtgcag aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac
     2461 ggaaaagaac tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagttgatc
     2521 aatctagccc ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca
     2581 gaagaaggaa atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc
     2641 gtatcacaca tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg
     2701 atcgaccaat ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa
     2761 gctgctagct ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta
     2821 gcaggattta ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct
     2881 ggtgcattag ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta
     2941 gctggttatg tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat
     3001 ggaattaaaa cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg
     3061 ttgctggtca atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt
     3121 atgagtggtt cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat
     3181 ttaggcggtc caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact
     3241 gttgcaactg gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca
     3301 ttggcaattt tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat
     3361 gctggtttga cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt
     3421 gcagcagctg acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc
     3481 ggattagtcg gagctttcgg aatcaaatta ctggctcctc acggcgggat tttcgttgta
     3541 ttcttactga gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct
     3601 ggtgtgattt atggtttatt gagaaaagct cctgcagtag cagaagcata gaaataaaaa
     3661 agaagaggct gggttttccc agcctcttct ttttttatag aatcatatag aatcaagtag
     3721 tgtagatgaa tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa
     3781 tttgtgatag aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca
     3841 gtcaagataa ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc
     3901 tgatttcctc tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc
     3961 attaatccgt ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct
     4021 tttagtgagg cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga
     4081 ttaccgccga gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc
     4141 agatgattaa gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt
     4201 ccaatcgctg tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga
     4261 tccatcccga aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc
     4321 aatatcggaa ggaaaatccc ccctggaaga cccccaccgt aagaaatcat ggaaaaaacg
     4381 aatcgaagaa caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct
     4441 aaaacgattt gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc
     4501 agcaagaaag gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt
     4561 ggcaaagcca gcaagacatt ctgataaata aaacctaaga tgcctagaag tactcctaag
     4621 atcactaagg aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca
     4681 ggtctcaagc cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat
     4741 gtcagccaaa caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct
     4801 gcgataggag cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc
     4861 ttttcttctg aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca
     4921 agctgtatag aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg
     4981 ccaatgaact ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt
     5041 ccctctactt gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc
     5101 acgaaaaatg ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc
     5161 cgactcattt catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac
     5221 aggctaacga cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc
     5281 tgagtcttat ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt
     5341 ttaatttagc gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat
     5401 tttgaactag ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa
     5461 gaaaggatgt tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag
     5521 aaagaagctg ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga
     5581 aagacgagag tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct
     5641 tggaacatcc tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc
     5701 aatgcgattc tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt
     5761 gtgcgtattt taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc
     5821 gattcttctg aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg
     5881 aaaaaatatg atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag
     5941 acccctcagg attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt
     6001 tatgctgctt accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc
     6061 atgaatacga ttcgattatt tgaagaacat cctgattcac tcacgtatta tcaaaacaaa
     6121 ttccattata ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc
     6181 aatttattgg caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt
     6241 tacggctggc gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat
     6301 gctgcagtaa ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca
     6361 aaccaagtca ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa
     6421 gaaggaaaca agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc
     6481 gtagaccgga tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc
     6541 ttgtaccgga cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt
     6601 ccttataaaa tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta
     6661 gcgtatttga atgtgctggt aaatcctcaa gattcaatca gttttgagcg tatcgttaat
     6721 tcgcccaaac gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt
     6781 catgaatggc cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa
     6841 gcaggacagc aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca
     6901 tacttgacag tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac
     6961 ttaaaaatcc aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta
     7021 acggtcacac aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca
     7081 ccagaagaaa aactaacggt cttcttaaat gatctggcac ttgtttccga cattgacaat
     7141 ttagaagaag atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa
     7201 tttccagttg tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg
     7261 atggaagaaa gcgaactgga ggaagaacgc cgtctcgcat atgtggggat cacgcgagca
     7321 gaagaagcgt tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat
     7381 aaccgacctt ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg
     7441 cgtcctacgc ctaaaaaaac gcctgtattc gctaaaaaaa cagcatatag ctataaacag
     7501 ccagagacag cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa
     7561 cctggagata aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg
     7621 acgtcgaagg atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg
     7681 gctgcctttg cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat
     7741 gacgattgac gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc
     7801 tcgcgaatac tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata
     7861 cgcagaatta gtagcaatcg aggaacaata tcctgatttg atcacgtctg attctcccac
     7921 tcaacgagta ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct
     7981 ttacagtttg aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca
     8041 aaaagcagtg ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt
     8101 ttctttgcgt tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt
     8161 aggagaaaac atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga
     8221 accaatgaac atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt
     8281 gaaccaagac agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc
     8341 tggttctttg cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt
     8401 atacacagta gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga
     8461 gctagaaaaa attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga
     8521 agtgtggtca tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga
     8581 tggaatcgtc atcaaagtga atgaattctc gctacaagat caacttggtt ttactatcaa
     8641 agcaccgaga tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga
     8701 aaacatcgaa tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc
     8761 agtccgagta gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa
     8821 gttgaaggat atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc
     8881 agaagtttcg caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc
     8941 tacacattgt ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg
     9001 gtgcatcaat cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag
     9061 aaatgcgatg aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa
     9121 actagtagca gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga
     9181 caaaataaaa gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa
     9241 ttcggtcgag cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa
     9301 aattttagca gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat
     9361 tattgcgctt gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa
     9421 cgaagaagtc catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa
     9481 agggcttcga aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt
     9541 ccttactggt aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct
     9601 tggcgggaaa gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga
     9661 tgctggcagc aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca
     9721 aatggcagat gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag
     9781 acaaatttca ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt
     9841 tttaactcgt ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt
     9901 tgccaaatta gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt
     9961 aggtaaaatc atcgatatgg ttgaaatgct ggaagaagta gatacggaag gtgttccatt
    10021 tacgtcgaat gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg
    10081 gaaccgcgat gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc
    10141 cgcaatcatc gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta
    10201 gaagaactgc atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaaaca
    10261 acatttgaac gaatcaaaga aacagagcct gaggtcgagg cattcattac attgaacgaa
    10321 gaaaaagcat tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc
    10381 cttgctggta ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc
    10441 gctgcatcta agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa
    10501 atctatcaag cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt
    10561 ggaagtacag aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta
    10621 cctggtggct cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct
    10681 ttaggtagtg atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg
    10741 atgaaaccta cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt
    10801 gaccaaatcg gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc
    10861 agcggttatg acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag
    10921 ggactgacag gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa
    10981 ggtgtggccc ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta
    11041 ggtgctgttg tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat
    11101 atcattgcct catcagaagc aagttccaat ttgcaacgat tcgatggtat ccgctacggt
    11161 tatcgctctg aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga
    11221 ttcggtgaag aagtaaaacg ccgtatcatg ttaggtacat tctccttaag cgcgggttat
    11281 tatgacgctt acttcaaaaa agcaggacaa gtccgtacat tgatcaaacg agattttgaa
    11341 aaagtctttg cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga
    11401 atcggtgaga acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct
    11461 gtcaacttgg ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca
    11521 gttggattgc agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat
    11581 gcgtttgaac aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac
    11641 gaatgaattt tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa
    11701 aaattttctc tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga
    11761 tcgactgggg ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga
    11821 tgaaagcagc tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa
    11881 attactttta cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg
    11941 gtcatgacgg atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac
    12001 gtgtccattt agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg
    12061 ttgatttgaa ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt
    12121 caccagaaga agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag
    12181 tcagcgatgt gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc
    12241 cttatggaca agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt
    12301 tcgtgaaaaa aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag
    12361 gagaaatcca acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc
    12421 gtgtcaaaga agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg
    12481 tgatcgatga cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc
    12541 gtcgcgaacg ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac
    12601 ttacaaaaga aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc
    12661 aagcatctaa ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat
    12721 tagctgagac aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg
    12781 gaacgatcag ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag
    12841 aagcccaaga agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac
    12901 tgccaatcat taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg
    12961 gaaaagatcg tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag
    13021 caaatcctgg tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga
    13081 aaatgaaaaa agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa
    13141 atttggcgga tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa
    13201 ctacaccaga accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg
    13261 atctagtagt ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc
    13321 cgcttgaaca ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc
    13381 gagcattaaa aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc
    13441 aaaccgtaaa aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag
    13501 gtggctattt gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt
    13561 atcttgctta tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc
    13621 gcttgaagta tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga
    13681 cgaactctgt tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt
    13741 tttctttaat cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca
    13801 tgctgaatgg cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt
    13861 tgttcgttga aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat
    13921 acggcgggga agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca
    13981 ataccgatca gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg
    14041 tcagcaaaga attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg
    14101 gaaaaatagc agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta
    14161 gtaaggtcag tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga
    14221 ttgaaaaata ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga
    14281 acgatataca gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga
    14341 tggttatcca ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt
    14401 aaaagcaggc aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga
    14461 tcgtcagcct gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt
    14521 gaagtatgaa caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat
    14581 tgctaagatg ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta
    14641 ccgtaataaa gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta
    14701 caaaaagaat agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat
    14761 cgatcaagca atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa
    14821 tgaagaaaaa aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca
    14881 tgaaatgatg gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc
    14941 tgaagctatt catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga
    15001 aaaaacaaat gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga
    15061 agatcagcta ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac
    15121 tcaacaagca gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga
    15181 cattgtgatc gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt
    15241 ggataaggtg tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc
    15301 tttaaataat aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa
    15361 aaaatggcaa gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg
    15421 attagatcat cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat
    15481 ctcatgtaat ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc
    15541 cgtgaagaaa gttcagcctg tagatctttt cccgcaaacc catcatatcg aattagtcgc
    15601 ttcattcgtt aaaggagaat aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa
    15661 aagaaacaag gtggaggccg atccaaacaa atggaaacac ctaagaatga tcgtaaaaac
    15721 atgagaaaag gccctaaaat ctttaataaa tagacgtgct aaagaaagac taagaagtgt
    15781 taacttttgt tattgtttct tagtcttttt a
//
LOCUS       DAZUKR010000056        15663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000056 DAZUKR010000000
VERSION     DAZUKR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.056"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACG89C_001576"
     CDS             138..1475
                     /locus_tag="ACG89C_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS4900120.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACG89C_001577"
     CDS             1523..3652
                     /locus_tag="ACG89C_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS4900121.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACG89C_001578"
     CDS             3627..4316
                     /locus_tag="ACG89C_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900122.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACG89C_001579"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACG89C_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACG89C_001580"
     CDS             complement(5546..5740)
                     /locus_tag="ACG89C_001580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900123.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACG89C_001581"
     CDS             complement(5790..6230)
                     /locus_tag="ACG89C_001581"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4900124.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACG89C_001582"
     CDS             complement(6234..7079)
                     /locus_tag="ACG89C_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS4900125.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACG89C_001583"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACG89C_001583"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS4900126.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACG89C_001584"
     CDS             complement(8703..9650)
                     /locus_tag="ACG89C_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4900127.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACG89C_001585"
     CDS             complement(9873..10223)
                     /locus_tag="ACG89C_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4900128.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACG89C_001586"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACG89C_001586"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS4900129.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACG89C_001587"
     CDS             11646..11966
                     /locus_tag="ACG89C_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4900130.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACG89C_001588"
     CDS             11988..12452
                     /locus_tag="ACG89C_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4900131.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG89C_001589"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG89C_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS4900132.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACG89C_001590"
     CDS             complement(13301..14590)
                     /locus_tag="ACG89C_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS4900133.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG89C_001591"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG89C_001591"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4900134.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4907 a         2912 c         3207 g         4637 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgt
//
LOCUS       DAZUKR010000057        15476 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000057 DAZUKR010000000
VERSION     DAZUKR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.057"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..438
                     /locus_tag="ACG89C_001592"
     CDS             88..438
                     /locus_tag="ACG89C_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS4900135.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            537..1067
                     /locus_tag="ACG89C_001593"
     CDS             537..1067
                     /locus_tag="ACG89C_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4900136.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1221..2372
                     /locus_tag="ACG89C_001594"
     CDS             1221..2372
                     /locus_tag="ACG89C_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4900137.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2481..4520)
                     /locus_tag="ACG89C_001595"
     CDS             complement(2481..4520)
                     /locus_tag="ACG89C_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4900138.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4578..5135)
                     /locus_tag="ACG89C_001596"
                     /pseudo
     CDS             complement(4578..5135)
                     /locus_tag="ACG89C_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(5283..5449)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(5674..5880)
                     /locus_tag="ACG89C_001597"
     CDS             complement(5674..5880)
                     /locus_tag="ACG89C_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900139.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5896..6510)
                     /locus_tag="ACG89C_001598"
     CDS             complement(5896..6510)
                     /locus_tag="ACG89C_001598"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4900140.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6657..7340)
                     /locus_tag="ACG89C_001599"
     CDS             complement(6657..7340)
                     /locus_tag="ACG89C_001599"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4900141.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7449..7907)
                     /locus_tag="ACG89C_001600"
     CDS             complement(7449..7907)
                     /locus_tag="ACG89C_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4900142.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(7991..9754)
                     /locus_tag="ACG89C_001601"
     CDS             complement(7991..9754)
                     /locus_tag="ACG89C_001601"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4900143.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9975..11435)
                     /locus_tag="ACG89C_001602"
     CDS             complement(9975..11435)
                     /locus_tag="ACG89C_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900144.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11643..12569)
                     /gene="glsA"
                     /locus_tag="ACG89C_001603"
     CDS             complement(11643..12569)
                     /gene="glsA"
                     /locus_tag="ACG89C_001603"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4900145.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12592..14019)
                     /locus_tag="ACG89C_001604"
     CDS             complement(12592..14019)
                     /locus_tag="ACG89C_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4900146.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14050..15264)
                     /locus_tag="ACG89C_001605"
     CDS             complement(14050..15264)
                     /locus_tag="ACG89C_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4900147.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4423 a         3093 c         2661 g         5299 t
ORIGIN      
        1 ttgttttttt gaataatcgc tttttcttct ctttttctta tatactataa gtagtaaata
       61 atttgaaaaa aagaaaggtg actgattatg gcgcaaaatc gtatttttac gacaagtttt
      121 gcaagcgttt atcctatgta tgtagcaaaa gcggagagaa aagatcgtat aaaagaggaa
      181 gtagatgaaa tcatttgttg gctgacgggt tattcccaaa cagaactaga atatcaacta
      241 caaaaacaag tgactttcga acaatttttt gcggaagccc ctgtccccaa tccttcacgt
      301 tccttgatta caggtgttgt ttgtggtgta cgagtagaag acatcgagga taacttgatg
      361 aaagaaattc gttatttaga taaaatgatc gatgagctag caaaaggcaa aaaaatggaa
      421 aagatcctca gaagttgatt tttccagaga tattcttctc cttcgctcat tttgtaacat
      481 ttaatttggt aaaatgacta gttcacgtta aaataagtca aaggaggttt tcttatatgt
      541 ttgagacgat taaacaaaat attcaaaaat acgccttatt aagagctgct gtttatatta
      601 tttctgggat tgcgattgtg ctaaaccctt ctgctgtttt tcattttatc ggttatctga
      661 tcaccgccta tcttgttttg cttgggttgt tgaatctttt tgaggcttac cgttttaaaa
      721 ggaaaacagg ttcttggagt atcggtctgt tcagtggtct ttttttcctg atcttagctc
      781 tgatcgtcta tgcttttgca cctgcaatcg tttctatcct gccgattctt cttggattag
      841 ctgttatcat caacagcttg ttccagttgt tcttcagcat gaatacaaaa gctaaaagtt
      901 ggagtgttta tagtagtatt ctgttgttga ttggcggatt cgtcttattg ttcaatccat
      961 tcaaaagttt gatgcttcta ttccaaatat tcggctttat tttgattttc atgggcatcc
     1021 aggagattat caatttcatc agaataagaa aaatgactat acgttaatca tttttagtta
     1081 ttaaaataga ccgtataaaa accaacaatc caaaaaaatc ttttggattg ttggtttttt
     1141 tgtgagtata ttttcctatt ttttcaataa tgtatgttat gctaagttcg ggctatattt
     1201 tatacaaagg aatgatgaga atggaattta ttggtatttt atgcctgata ttgatttcaa
     1261 caacgattgg ctcacatatc tcgcggagat taggtatccc agcagtgatt ggacaattac
     1321 ttgttggtgt tttgctaggc caagctggat tgaattgggt caaacctgat attctagtcc
     1381 atgacttttc ggagattggt gtgatcttat taatgttttt agctggtctg gaaagtgatt
     1441 tatctctttt gaaaaaatat ttccgacctg gtatgttcgt tgctttgctt gggatcatct
     1501 tcccggttgt ttttggctgg ttgacaggca aagcattcca agtttcggga aatgaagcga
     1561 ttttctttgg gattattctt gcggcaactt ctgtcagtat ttcagtagaa gtattaaaag
     1621 aactaaacgt agtgaataca aaagaaggat caacaatttt gggtgcttct gtggtagatg
     1681 acattttagt cgttttagtt ctaagtttca gcctatcttt tctaacgggg gaagcttcta
     1741 gcaatgcttc tttaccgctt gttttgattg aacagctaat ttattttgtt tttattttct
     1801 tattagtcaa atggattgcg cctttcctac tttcatttgc agaaaaagtg tacgcaaatt
     1861 cttctattat catcgtatcg ctagttattt gtttaggaat gtcttacgta gcggatcttg
     1921 ttgggcttag ctctgtcatt ggtgcttttt ttgctgggat agctgtcagt cagacaaagg
     1981 ttaaagaaga agtataccac aatgtagagg ctttaggata tgcagtattt ataccagtat
     2041 tttttgtcag tgtaggatta gaagttgatt tttctgactt tagtgaacaa ctactcttta
     2101 tttctatatt gacgcttgta gcgattttga ccaaattgct tggaggttat tttggtgctc
     2161 agattgccgg tttttctcaa catagttcat tgatggtcgg tgctggtatg atttcgcgtg
     2221 gagaaatggc tttgatcatt ttacaaattg gacagcaaag tcaattgatc gaaccacagt
     2281 actattcacc tttagttatc gtgattttat taagtacact tatttctcca cttgttctca
     2341 aatattttac aaaaaaagtt tatgaagtgt gagtaaatca aaaagaagtt gtgaaagaga
     2401 catcgtgaaa accgtttatt tgaaaaaagg actgtgacga taacgccaca gtccttttac
     2461 tgtacttaaa aagcaatttt ttattgataa ttttggttga ggtattgcaa tagttccgat
     2521 gctcgtttgg ttgctgaatc atcatcttct ttattttcca acatgctgac catcatataa
     2581 ccttggttat caagattgaa agcaaacaag aaactgttct cttgcccctt tacatcctgt
     2641 ttttctttga tttccgctgt accagttttc gctgccaacg gaatccctaa agcagaaaga
     2701 gaatgtgctg taccatttac atcttgcaca acttctctca gatctggcac gatcgtttgt
     2761 actgctgttt cgctgatgac attcttctta tcttttgtct ctttatctgc aatcaattta
     2821 ggatagacaa gtgtgccatt gttggtaaaa acagaataca ttgctgcttg ctggatagga
     2881 gtaattagaa gttcgccctg tccatatcca gtatcagcta gcaagatatc tgagttaaag
     2941 ctatcttcat tagaaatttg tgctggattc atactaattg gcaaatcaag gtcttcacca
     3001 aaaatgaatt tatctaagcc tgtacgaaat tttttctcac ccattttcaa cgtttcttgt
     3061 gccgcatata tattatcgga atagatcaaa gcagttttta agtctacttg tgatacatca
     3121 ctactgacac gcgttgtttt aaaagatccc caagaactgt ctttttgcca tttaagcccg
     3181 ttgatcgtca acacttcatt tggatcgata gtgccgttgt cgagaccgat tgctgctgta
     3241 atcattttaa acgtagatcc aggagcataa cctgtcgcaa atcggctgat gaatggatgt
     3301 tcaggatttt cttcataagc tttgtaatct tcttgtgaga tcccgtttgt cattttgttt
     3361 ggatcatagc ttggagagct agcaagcgca agaagatcac cggtttttgg cgttgtcgca
     3421 acagttgatg cagcttttcc tcctagactg tcaaaagcta ttttttgtgc cttcgcatcg
     3481 attgtcaatt tgatatcttt gccattttga acttcatgtt cgatcagaac ttttttctct
     3541 acaccgtctg catctgtaat gcttaatttc ccacctgttg tcccacgaag atccttatca
     3601 aaagccatct ccaaacctga gcgaccgatt tttccattac tgcttaattc tggattctta
     3661 tcaatatctt ctgctgtgat atctcccacg taaccaatca gctgagcagc tgcttcaccc
     3721 aaaggataat atctgccgtc tacttcttga atggtagctc cagctggaag ttctggcgtt
     3781 gctccatcaa tgattttcaa tgggacaaag taatcgggtt gtacccagct ttgtgaaatc
     3841 gcctgattga tagcatcttc tgttaagtcg aaggaggaag caatcgcttt gatattggct
     3901 gttttttcgc ctccatctcc aagtttactt ggaacgactc ctaattgttt tagtttgcct
     3961 gttgttgcta atggttcacc atttcgatct ataatattcc ctcttgctgc ttcttgtgtg
     4021 gtcaggctta ctttgtcatt tccttccatt tctggaaaaa ccaagttggg ttgccagttg
     4081 atcgtggttt gaccatcatt tctgtctaat gttcctttat aagaaagatc tttcaattca
     4141 cctaatgagg tattcatctt tgctttatat gaaaaactgt aagtagaatc gtctttttta
     4201 tctactttta gattcgatat ctgaagtcct ttgacatcag cagcaccgta tatattctgg
     4261 tatttatcta agatctcttt ttcagataat gcacttttat ttgccgcctt ttttgatgtc
     4321 atttctgcag ctttgttata atctcccttg ttcaaagctt ggacaaattg ctcaactgtc
     4381 ttttctccag cttctactgc ttgggtttct tgatagtgct ggtagtaaaa ataaccgcca
     4441 cccgcagctg ctattaagat cactgcgccg gcaatataag cgcctgttcg atttttgccg
     4501 tgcttgtcac ttcttttcat tttttcctcc ttctcttgct tgtcattcct ttatttcact
     4561 atttatacct gtttattcta acatatgtct ttgaggaaat ccataccaca atccagaagc
     4621 acactttctg gaaggttcgt ttggatacta ccaagtaatt tcccaaccgc tttgggtagc
     4681 ttgaatattg aagttacgtc tttcagctgc tgagagatag ctttgatcac ttgctgctgc
     4741 cttctgatcc tcccgaagtc tccttctttg tcttcccgaa atcgagcata ctggagtaaa
     4801 gtattaccat ccatcacttg ctctcctttg tttatatata cgccatctaa atctagagat
     4861 ttttctgcat cgatcttgac tccaccagga aacagttcat taacgcaatc gataaaatct
     4921 tgaaatgtga tacttgcata atagggagct tcaaatttga aattttcctt taatgtttga
     4981 tttaataaat caactccccc ataagagtaa gctgcattta tcttattcat tccgtaacct
     5041 ggaatatcga catatgaatc tctcatgata gagatcagtt ttgcttgttg tgttttctgg
     5101 ttgtattgtg caaccagaag tgaatccgag cgacctccac caatcgatct tttttcaccg
     5161 catctcctcc tcacacagca atccacttgt caggtgagga gcgagtttgt ctcatcaaat
     5221 aagtcagtca caaaaatata aagaaaaggt cagaaacctt ttcgaacaag aattctgacc
     5281 tagcatcatc tcatcgtccg agttttagca ctatacaacg taaagaaaac attcttagct
     5341 attggaaaaa gccttaatcc aacagtttct gttttaccca ttggcgactt tcgacgtttc
     5401 tgggcaacag cctatgtttg tataggagcc tcacctaaca aggttattac tatattctat
     5461 ttacgagttc atgatagact tttcctaatg attaggcaat ggaatttgaa atgaaatagt
     5521 gaaatttaac aatctttaat aatttttaga aaaaaataac aaataagttg aaagaatccc
     5581 gttgcaaatt ctttcagttg caccaggatt ctttgataat tttttagttt tatgtgtttt
     5641 tttatatgtt ttttcacttt tcggaataaa tatttatttg ttaaagtaat taaacaatcc
     5701 gctaatcgtt tgatctggca tttctatttc cttaccatct aaagtaacag tagtaacgac
     5761 aaaatggtct tgcaatggcg gaacttctaa gtactgccct tgcagaaaat cattcactgt
     5821 ttcatattct tttttcattt cttttgaatc tgtcgatggt ttatagctta tttttaacat
     5881 aactagtgct cctttttatt cgggacgtgc aatcgtgaaa atttctccta ttgctgcata
     5941 atcatttcct gccaaacggc ttacagcatt caattttctt ggatcgatcc ttcctgtctt
     6001 atcatcatag actgtttcat ttaaatgata tttctgtact tctagcaaaa ataaatcagc
     6061 tgttacttta tttttattcc gtatctcgac atgttcatat aaggatgttt caaaacgaac
     6121 ttttgcttct gtcaatgctg gtactgaaat gattactgaa tcagttaaag tcaagtcagt
     6181 caggtacagt tcgctttcct cattaggcag attggctgct gttttattta cttcttctac
     6241 gttgtcctca tcaaggatat ggataacagc ttctcttgtc tcaagaatat tctttgctgt
     6301 gtcttttact gcatcttgtt ttctctggat cgccaaggat aagatgggtc gattcgaact
     6361 gacgatattg aagtaactga agggtgcgat attcactata tcttttgctg atttagtcgt
     6421 tactactgct actggtctag ggatgactga accaatcagc aacttgtaat tttcacgttc
     6481 tgtcaattct gaaggactcc gttcaaacat ggtttcgcct tctttctata aaattcgtta
     6541 aaaagcagat cttgaagaca aaatgaaaag taatgctttt ttcatcttct tagaatctgc
     6601 tttaaactat ctatcaattt ttcttttttg tcaagaatct gtgccgtgcg tgttttttag
     6661 ttatttgctt tacttgcggc ttcgacatat cttttagtga tcaattgcgg acgggtgata
     6721 atagatccaa ctacgactga aaaagcacct aattcatatg ctttctctac ttgttcagga
     6781 gagtcagtat gtccttccat aattactggg atatcgactt gttctatgat ttgattcaat
     6841 aaatcaaaat caggtgtctg cgtctttcgt gaatattctg tatagcctgc taaagtcgta
     6901 gacaaaagat caaaaccatc ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct
     6961 gccatagcta accgaccgtg ttcgtgaatc atttggacaa gttcagcagt tgttttttct
     7021 gtcggacgtt tacgagaagt tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt
     7081 tcttccactt ctttttctgt cgcagtaata aacacttctg acccatcgta atctcttttg
     7141 ataatcccta ttaccggtaa atcaacgatt cgcttgattt cattgatatc ttctgtcgta
     7201 tttgctcgaa taccaactgc tccgccttct tttgcagcca aagccattct ccccataata
     7261 aaagaactgt gtagcggctc gttttccaaa gcttgacaag aaacgacgac tccgcctttg
     7321 atttgttcca gtgtatccac aatatctcct ccagtaatgt tatataaaaa tacagcttca
     7381 tataaaaatg taaccctttt ctattcagga gacaataatg agctatttct ttcagcagac
     7441 ctttcttact aaaagaaaat tacttcaaat tcttcgtata cgaccagctt ctcttctgta
     7501 aattctatgt tcaacgcttc tttataagca tgtttaaaaa actcagcatg gactttccgc
     7561 caataagcta atgtccgatc cccttctcct tctttccaag catgctcttt cgtgacttca
     7621 ctaaaaggca agatggtcac atttatattt ttgatgatac aaactggttc attttttcca
     7681 ttcaggacca cgtctaattg accaactttt ggtaaaggct cattctcttg tttatacaga
     7741 tcgtaaccac ttgatgtacc agtctttatc ttttttacaa ctagttcgct caactcgtct
     7801 gccatttttt ctgtatcccc aaatgaaaag aaacctaatt tttctgattg ataactagga
     7861 tatttttctt ggaacttttt gacatattct tctacttgct gatacataac atccacccct
     7921 ttgcttttta taatgaaaaa accatccaga tactagtata aacaattatc tggatggtga
     7981 gcataatgaa ttattgaata ttcaagacac gcagactatc ttcaacgatt tgttttggtg
     8041 ttaaatgata acgttcataa agctgcccta caggaacgct atccgtgaat tctttctctg
     8101 caccaaaatt taatacgttc attgaagaat caccataata acgactgata ttttcgccaa
     8161 atcctcctga caaactacca tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt
     8221 tatctaatgt ttctacgtca ttttctgtga tcgttcctgg attgatcaac gttgcttcga
     8281 ttcctttttt tctaaaagct tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag
     8341 ctaaaattgc tacttttcct ccattattta ctgtttgata ggctggaata ctataatcat
     8401 ttgcgatagc tgtaccatgt tctacaccat gtgtaggaat tcggattgct acaggctgcg
     8461 ctgtttgttc gaacgcccaa tctagcatag agatcatatc ctcttttgtt gctggagcaa
     8521 gatatttgat gttcgggata cttgataagt aaggaatgtc aaatgttcct tggtgtgttc
     8581 ggtcactagc tgagatcgtt ccgccaccga ctaaaataat cactggattc tcgttgatcg
     8641 ctaaatcatg tgataattga tcatatgccc gttgcaagaa agtacttgat tggaagacga
     8701 ctggacgtac tccctgagaa gccaaagcag aagcgaaagt gatagagtgc tgttcagcga
     8761 tccccacgtc aaaataatga tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc
     8821 ctggtatcgc tgcattgact gcggcaatag gagctgtact tgtttgcatt ttcttatcca
     8881 aataatccat gatgatcata ttaaatgttt ctccagaagt tacttgcttt gcctccccgg
     8941 tggacagatc aaatggtaca tgccaatgat atttcatttt attatcgatt gccggctgat
     9001 aaccgcgacc tttttccgta tggacgtgaa gtacaatcgg atgatcaata tcttttactt
     9061 cttcaaataa acggatcacc gtttccagat cgtttccatt atccacgtaa cgataatcaa
     9121 atcccattgc tttaaatagg ttattgctgc ttttaccgtt ggtttgacga agctcagcta
     9181 agtttttata taaactgcca tgattctcat caatagacat ctcattatcg tttactaata
     9241 cgattagatt ggattgtaaa acaccaccat tgttcaatcc ttcaaaagct aagccaccac
     9301 ttagggagcc gtcaccgata aatgctacca catttcctga ttctttcttc agatctctcg
     9361 ctttagctaa acctgttgct aagctgatag atgtagatgt gtgccccact gtaaaatagt
     9421 catgtttact ttcatgttgg ttggaatatc cgctcacttc acggtaatgt cctttttcaa
     9481 aaccattttt tcgaccagtt aaaagcttat gaccataaga ctgatgggaa acatcccaaa
     9541 cgattttatc ctttggtgaa tcaaatacat aatgaaacgc aatcgttaat tctactacac
     9601 ctagattcgg accaacgtgc ccacctacat cgctgtcctt ttgcaagatt ttttggcgca
     9661 tctcttctgc caactgtttc atttcctcaa cagataattt tttgacatct gcggggctat
     9721 tgattgcatc caatacatgt aatactgtac tcataataca ttctctcctc cgtactatct
     9781 tcttttaatg aaaatagatt tgttacactt tatctattct actacttaga gttgactcta
     9841 agtcaatagc attttaaaaa aatttcagag aaagcttgtt atataagtag atcttcgcac
     9901 gaatcaggac tcaaataaaa accttctaca gacaattaaa aagctttgta gaaggtctcc
     9961 taattaaggt ctcatcaaga aatatgtttt tttaataggt gaagttcctg agcaatatga
    10021 tacaaatgga tgtttgcacc ttttaatgag attggataga tctttgtctt ttcagtttct
    10081 aatccatttt gatagctagc agcatcgcac aaaaccactc tttttttcgc acatagacat
    10141 tcatcaggca attcttctaa tgaacgaaat aaaataggat ttatttcagc gatgccttct
    10201 tccgcataca aatgaaattg ggttcgataa aaaaattgtt tgactgaact ttctgaaaca
    10261 acaaaaattt ctatttcctg tctccccgct tgttcactgt tgatcaaaga aagacttttg
    10321 acgaattttc gaatgaaatt ttcattccat cttagttgga tttgattttt ctttttccac
    10381 tcttccaata tttctacaat ttttccgtac agaagttgtt ctttttctga tagaagatag
    10441 atcctttccg gtaaaaatac ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag
    10501 gattgcttga aaataaaatt ttctgcatcg tcgatttcag cgatactaat aatctgtaaa
    10561 atcaattcat tcagtgatag gtcattgaga acaaaatttt tataaacaac ttcgaaatct
    10621 tttcttaaca gctccatgtt ctcattggta taatttctac tgttgaacaa agaaaaaata
    10681 taatagacat cttcttcttg taagtctaga attccttttt gctcttccaa tccttgacag
    10741 actaattgaa ataacggctt cgtttctgcg cttttcttat cttcttcttt cagtccaagg
    10801 ggaaatgcct ttcttccgat tgcgatctct attcctcgta tgataaagga cttttcatct
    10861 tccgaatatc ttcttttaaa aaaatgagtc tctacataat caatcaactc gttacaagct
    10921 tcttctacat acggattttt attttcatag ccttcccaat tgacataacg tactaaagct
    10981 agcaagatgt tccgcgagtc aaattctggg ataatgattt ctttattttt caaataatca
    11041 ttttctttaa aaaaatccaa tacgattttt ttgattgtat atactttgct caaagaaata
    11101 aattcaagat cagccagttc tgtactcgtg aatataccat taaaataata acttaaaaac
    11161 tttaaaaaat aagattgacc gtacagaaac tgtgtgagct cagaaagagc aataccatgt
    11221 ttgaatacga gtttatactt atgcttctct cttacttgtt cttttataca atattctaga
    11281 cactcggcta gactttctag atcgtgaagg atcgttggtg tagtcaccct tagagaaata
    11341 gctgtttgtt cgatgtctat ttcttggaat tcaaataatt gttcacacag atacactttt
    11401 ctaaatatat ttcgctcgat gtaattttcc agcattcctg atacacgcct ccagtttttc
    11461 ttatgatttt atttaatcat atctgtaaat cgtatttttg tttccttttt atttagaaaa
    11521 gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt
    11581 cagcctcatt gtaggtagct ttgagccgtt tggactttta ccccagcctc tttcaagaat
    11641 ggttattcaa agatgtcagc gtctagttct ttgactacat ctttcaacaa ttggatacca
    11701 gctgcgctgt taccaaatgg atctagtgcc gggctgaaga cacctatgcc gtaacgattt
    11761 ggcacagctg ccatcaaccc gccgcctaca ccgctttttg caggtacacc aacatggacg
    11821 gaaaactctc ctgattcatc atacaaacca gcagttgtca taattgattt gacgattgta
    11881 gcactttctt cagtaatgat ttgtttgcca ttccaaggag caattccttt atttgccagc
    11941 acagcagcta attttgctat gcctgtagcg gtgacattga gggagcattg tctgaaataa
    12001 gcatcgagta tctccggtac gtctccttcg atcatttgat tacctttcat atagtaagca
    12061 agagagcgat tgatatcacc tgttcttgat tcagaatgat agatttcttc attcaatgtg
    12121 atattaggat catcacagat ctctttcata aatgacaaga ttcgctcaaa tttttcatct
    12181 gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc
    12241 attggatgtt tgcgatgatt gatttccata ttcatgatag aattgaaggc aaaaccggtt
    12301 ggttccgtgt tgattgtttg aaagactttt tctattccgt tgtctttgat tgctaataac
    12361 aatacgggaa cttttgacat gctttcgatg gcaaagcgca catcggcgtc tcctccctca
    12421 atcagttgtt ttgtatccaa atcataaatc gccataccta attgtttggg atccacttca
    12481 gctaatgctg gaatgtaatc cgccacttgt cctgtttgat aatagtggaa attttttcca
    12541 atcaattgtt ctaattcttt tacttccatt tgaataacca gctttcattt tttatttttc
    12601 tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat ggaataacta agacgataag
    12661 ataagaaaca atcagaatcg tttcataaat cccatgctgt ccagctgcga tactagcagg
    12721 tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat
    12781 tacttttaca acttttccgc ctggaacctg ataggctctt tcaagactgt catctttaaa
    12841 aaccagcttc aaatagcccg cgaaaaacat aaagtacaac atcaggtaga ttacaacagt
    12901 caatgacatc gctgcaagaa atgaaagatt attccccccg ccgcttaatg taaagacggc
    12961 tgcccagatt gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa caccgtgtgt
    13021 gttttctttt gcaaaatatt caggaagtaa tccttcttct gctgctacgt gaagtgcttt
    13081 tgaaggagag gttacccaag aagcaatttc tccgatgaca cctaaacaaa caagaatcgc
    13141 aagaattttc gcaatccatg caagatttgg atcaatgtgt cttaatagga attctaacga
    13201 ttgaatcacc cctgtattaa gagacaaacc atgttgaggt actgtagtag cgacggtcaa
    13261 tccgccaaaa gtatctaaaa tgattgccag aataaccaat acaaacattg ctaatggata
    13321 atccttttta gggttttcca tttcatttgc atgactggca gatgtttcta cgcccatata
    13381 tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa
    13441 tgttttcata gacagtatcg tttgtagtgg agcgcctgaa gcgacataat ggatccctaa
    13501 gccaaacaag actaatgctg gcaacaagat accagctaca aaaccgatcc gagcaatcaa
    13561 tgaagtgtat ttggttcctt ttaactgtga gagtaaaata gcccagaaaa taatcaatag
    13621 gattgccaat ttcagatatt tattgttttg tatagcacca atacccgttg cataggataa
    13681 tgcaccagtt aagaaataca gcatggtcaa gtagccaact gttatctcaa accattggaa
    13741 aaagattgcg gcaaaaccga atcgttttcc caatgtgcga ctgacccaag tatagacacc
    13801 gcctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
    13861 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
    13921 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaacgt
    13981 taattttttt gcaggaattt tcgttttgct ttgttgcatg aatcttcctc ttttctattt
    14041 gaatttgttt caattgagtt tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc
    14101 ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag
    14161 cacataggca tacacggcag taatgatgac accaatcact tgaatcccta agaagtacca
    14221 gctgcttgga ttcgttagta aatggctgtt agatgcaaag atccctatca gaatcgtacc
    14281 tgtgaatccg cccatgccat gaacacccca cacatcaagt gcatcatccc attgtttctt
    14341 tttacgaaaa tcaacagcag catgacagac gatacccgca atgatcccaa tcagcatggc
    14401 actttttgct tctacataac cggcacaagg cgtaatggct gccaatccag caacggatcc
    14461 tgttagtacg tcgacaaaat caaagccatt tccttttact tttgcaatga tcagccaagt
    14521 aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat gcattgactg cttgttcatc
    14581 tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca aaccaaagca gtcctgtacc
    14641 gatagcagct gccataagac tgctatgctg acttctttct cctaaaggaa tctttctttg
    14701 accaagtgtg aggatacatg ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc
    14761 accagcgaaa tctctaaatc ccatctgatc caaaaatccg ccaccccata tccagtggca
    14821 aacagggaaa taaatcagca gattccaaaa aataacaagt ataatatagc cttttaaatt
    14881 caatctccct gcaaaagctc ctgtcatcaa aggaacggta atcacgataa acatcagttg
    14941 gtaaagaaag aacattaaaa atggaatcgt tgcaccatga aacatgttgg ggaagcttgt
    15001 aacatgtctc atcaaaaaga aatctgccgg gtttccaata acaccgccga tatcccgtcc
    15061 aaaggccaat ccgaatccac caaataccca cataatcgtt gttatcccaa cggagagaaa
    15121 agattgagac atgatcgtta gtatattatt cttccgtgct aatccgccgt aaaaaaaagc
    15181 tagaccagga gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc
    15241 tgtattgata ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa
    15301 gaaggtgtag ttgttttttt cttctgtttt tttgaattaa tacttgcatt tataaaagac
    15361 agacaattaa taaaagcttg atgtgttgtg ctctttttcg tttttcacac aaataaagac
    15421 aaaaaataaa cggtcttctt tttatagaaa accgtttatt ttcgtctttt tttatt
//
LOCUS       DAZUKR010000058        15446 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000058 DAZUKR010000000
VERSION     DAZUKR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.058"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1807)
                     /gene="uidA"
                     /locus_tag="ACG89C_001606"
     CDS             complement(<1..1807)
                     /gene="uidA"
                     /locus_tag="ACG89C_001606"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4900148.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(1835..3601)
                     /locus_tag="ACG89C_001607"
     CDS             complement(1835..3601)
                     /locus_tag="ACG89C_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS4900149.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(3614..4408)
                     /locus_tag="ACG89C_001608"
     CDS             complement(3614..4408)
                     /locus_tag="ACG89C_001608"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4900150.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(4419..5795)
                     /locus_tag="ACG89C_001609"
     CDS             complement(4419..5795)
                     /locus_tag="ACG89C_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4900151.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(5924..6571)
                     /locus_tag="ACG89C_001610"
     CDS             complement(5924..6571)
                     /locus_tag="ACG89C_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4900152.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(6655..8082)
                     /gene="uxaC"
                     /locus_tag="ACG89C_001611"
     CDS             complement(6655..8082)
                     /gene="uxaC"
                     /locus_tag="ACG89C_001611"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4900153.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(8156..9235)
                     /gene="uxuA"
                     /locus_tag="ACG89C_001612"
     CDS             complement(8156..9235)
                     /gene="uxuA"
                     /locus_tag="ACG89C_001612"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4900154.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9238..10869)
                     /locus_tag="ACG89C_001613"
     CDS             complement(9238..10869)
                     /locus_tag="ACG89C_001613"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4900155.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11186..11857
                     /locus_tag="ACG89C_001614"
     CDS             11186..11857
                     /locus_tag="ACG89C_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4900156.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(12693..14147)
                     /locus_tag="ACG89C_001615"
     CDS             complement(12693..14147)
                     /locus_tag="ACG89C_001615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900157.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(14148..14447)
                     /locus_tag="ACG89C_001616"
     CDS             complement(14148..14447)
                     /locus_tag="ACG89C_001616"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900158.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(14750..15121)
                     /locus_tag="ACG89C_001617"
     CDS             complement(14750..15121)
                     /locus_tag="ACG89C_001617"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4900159.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         4858 a         3124 c         2127 g         5337 t
ORIGIN      
        1 attttttata actgaaattt ggtattgatg accatcgttt ctttagatag tgagcaatca
       61 ttttaggctt tctatctcta gtaaagattc ctttcttatt tccttgcact cttaaaatcc
      121 cttgacttgt ttcaaaatct gcaaaattcc acacttgttc accaacaaaa ttgtcacatt
      181 tatctattac ttcgtgattt gcttcgtaat aacccacttg atactcttct gtaaacattg
      241 ttggtactgt atcatgaagt ccagctactg tatctgctcc atattctgta aacattattg
      301 gcgtatcggg acatctttcc atccatccat ctagctcttt tttcaacaga tattttgcat
      361 gttttaggtc tccacctgaa acataccaac cataatagcg atttaaacat aaaacatcga
      421 caatatctcc tactttacaa atatctggtg ttgactttaa gtaagtaacg attgttacag
      481 gtctcttctg agggtcaagt tctttagtca attcaactaa aggttcaaaa tattctttag
      541 cgccttcaga atccgaatct ggttcatttg caatagacca cattacgaca ctcggatggt
      601 tcttgtcgcg ttctatcaat tccttaatcg ccattttgtg agcattttct gtccgtattt
      661 ctttccaagt atcatgtttt tctacgttgt catctaataa agtagccatg aaattcaaat
      721 gtaatcctac tgcaggtact tcatcaataa ctacaatacc ttctcgatct gctaagcgca
      781 taatttcttc tgaataagga taatgtgaag tcctaaatga attagcaccg atccatttca
      841 ttaaattaaa atctttgata tttactgctt cattcaaacc acgaccatta atatacgtgt
      901 cctcatgctt cccaaatccc ttaaagtaaa aaggtttatt attaatcaaa aacttcccat
      961 tcgaaacctg aatactacga atgccaaatt cttctgtgta cgtatcaatc actttatttt
     1021 cgttaattaa actaactttc atactatata agtaagcatt tagaggctcc caaagtgttg
     1081 ggttcttaag ttgaatcact ccttctttcc ccttagcttc tgctataagt tgagcttctt
     1141 cgtcatagat tgctattaga atttctccgg tccattctgt atcgatatta aattctacat
     1201 ttgcatctga atcaattatt tttgtattaa tagtgacatc ctttatatag tctttcggag
     1261 ttgtatacaa tttaacgggt ctattaatgc ctgcataatt gaaaaagtca aaattaacca
     1321 aattttcttt ttttatgtct ccatcttcct gtatcgtttc tttcaaaatc cctacaggta
     1381 aagtgctatc gtcaataaca ttgtttacag ccactgtaat tctgttactt ccatctttta
     1441 taaactgatt aatttctgat tcaaatggcg taaaccctcc tttgtgttga ttaatcaatt
     1501 ttccattcaa aaatactttt gcttcatgtg ttgcagatcc aaaacgaaga actaatcttt
     1561 cttctaataa ttttttagga agaatcactt ctttttcata ccacacccat ccaacatgat
     1621 ttctaatgtt ctctgtttca aataaatcat tataagaaga aggaactgaa attggataac
     1681 tctctttttc aaccattggt tctaaagcaa gttcttcttg taagtcatca tttgtatcta
     1741 acttaaatct ccaaagaccg cttaaatcaa ttacttgcct cgtctctgtc ataatgggat
     1801 ataacatgta aatcactcct atataattta aatattaaat aaatgtatcc tttaacccac
     1861 aataagcatc tattggactt attcccttaa attcagaaag gcccattagt ttgtccacta
     1921 atatttctaa agtttcttgt tttccatcat aagtattgat ataagttttt acttgaggca
     1981 catcagctaa atgaaacgga cactgtacag atacaaaaat tgttggaatt tcatgaatat
     2041 aaaatggaat atccggagta cctttacttg ctggccaaac aattctttga actgtacctg
     2101 ggttaacatt agctatatta ataatcaagt catatttttc agttaaatct ttgattggtc
     2161 tcttagcagc atatattcca gctatatttt taattttttc ttcttgcgat aatcctttca
     2221 attcttcttc aggtgaaatg tatttttcaa cttcaaatcc ttttgctgac aaaatctgtt
     2281 tcaataaatc aatggcctga ggtctatttc cagcaactac ttcaccaaac ccacctttta
     2341 ccccatgtac atctaccagt aaaatccttt tatatttttc tggtacaata ggccaaatat
     2401 cttgtttatc tttaactagt gtaattgctt gatcagctac ctctttaaaa attgatttat
     2461 tttcaattaa accaattttt tccatagctt cttgtttcgg taacaaaatt ttattctttt
     2521 cttttttatg caaacctagt ttagccttta agcctaaaat tcttcttaat gcatcacgta
     2581 aacgctcttc tgaaataatt cctttgtaat aaccttccat catataatta aagtcttcat
     2641 ctggatcatt aaaaaatagg aatagatcac atccagccgc aatagaagtg ggcaataatt
     2701 ctgaccgttt cattgcccca gtcattccta acatatgact agcatcagta actactaatc
     2761 cattaaaccc gagttttcct ctaagtaagt cagtgattaa ttcttttgat aaagttgcag
     2821 gaagtaaatc ttcatctttc agatcaagat taaaatgttt tgaataagct ggcatatgga
     2881 tatgacctgc cataattgaa ggtaatccag cttctattaa gcctgagtat acttttccaa
     2941 aagaattatc ccattcttct gtacttaaac tattaatact aaaagataga tgttgatctc
     3001 gttcgtcaat accgtcacca gggaaatgtt tagctgcagg caaaattcca ctctcttgaa
     3061 tacctttcat ataagctaaa ctaagttcta aaactttatc agggctttgt ccaaaagatc
     3121 gactagaaat gataggattt ctccaatttc tattaatatc tacaatagga gcaaaactcc
     3181 agttacatcc aatagccgat gcttcaattc cagaaactct ccctaattca taagcatatt
     3241 tcgcatcatt tgttgcacca atttttactt ctacaccaac ttcggtacca tctgtacaag
     3301 ctccgttacc acctgcttct gtattagcag caattaataa aggaattttg ctttctgttt
     3361 gtaggatata gttttgatca tatacctcat cagcagttcc tggattatat cgtacagctc
     3421 caatatggta ctgattgacc atactactta agtaattttt atcacgacta gatcccatat
     3481 taacaaataa ttgacctatt ttttcttcta aagtcatagc atcaatcgtc tcttcaatcc
     3541 aagaaatagc atcttcatct aaatgatatg gtttttttcg taagtctact tttatagtca
     3601 ttttctttct cctctactta ttagtaagat ttttccaaaa agtatctata tatttttgtt
     3661 caatatctat atactctccc gataaacatt taaataaaat atcagttctt aagttttccc
     3721 atgattcttt ttctttatta accaaaatag gataaaagaa aactttattc ttatttgcag
     3781 cttctagatc accaggagaa tcaccaatca ttaatatctt atccaaatca caaccatttt
     3841 caattacact tcgaataatg tcttcttttt ttcctttatc ttgacaacac agttcatcaa
     3901 ctaaggataa taattcatgt ctttcccatt cttcttgaat agcttcctta ttagctgagc
     3961 ttacaataat tatcttccca tattttttca gagcttttat agtatctcta gcacttaaaa
     4021 agggtttatc taatccttta gcttgactaa tctgtcgatt gacttcttcc gaccatttta
     4081 aagcttttaa taaatcctct gactgagagt attctatttc cctcttcaaa gatggattag
     4141 acaattcttt tgttacagat acccagtgct ttaatcgatt aatcgatggt acatcatttt
     4201 tttctaatgt tttaactaaa cctaaaaatc tgtttattcc acgagtctct gaatataaat
     4261 taatattatt ccaatcctgt aggaattttt ccctatgatg aacatcaaaa atctctgcag
     4321 caattggccc gaaaaagaga ttgtgtttat aagtcattgt atccatgaca caaccatctg
     4381 aatcaataca aaatatccaa ctgttcatgt tttttctcct agttatgttt ttgatttagc
     4441 aaattataat tttctaatac tttttcttta gataatggat atacgaaaaa taaaatcaat
     4501 cctacaacaa agaaacaaac tgctggaatc aacgttgaag agttatacag cttttgtaaa
     4561 acttctccac tttgtacttt tgctaacgaa tcatacccta ttatagaaag agtccaacct
     4621 ccgacaccgc cagctaaagc ttgtccaatt tttcgggcaa aagaatatac tgcataaata
     4681 gttccatctt ctctttggcc agatttcact tcttgatcat cgattacatc agtaatattc
     4741 gcccaaatga ctgtattaaa aatacctaac cccatatatc caacagaagt aagaactaag
     4801 aatacataca tattactagg ctttatcaaa aataaaagta aatatacaat agcagagaag
     4861 aacatcccta ctgacgccaa ttctttttta ccgtattttt tactgaaaat taaacttaca
     4921 ggtgaaacaa gtaccaacat caaaatagga ttgataaagt tcattaaaac tattccctgt
     4981 gcattattat aataatctgg aaatacataa ttgttcatac tcattgttaa aagctgagca
     5041 agcaacaaaa aaatagctgc agctattata gaaagcaacg ctctattttt agctaatcct
     5101 tttaaagatt tcaacaatga aacttttaac tgcgattcat cacttttaac ccgttctaca
     5161 gttaatttat aacatattac gtaacaaata actgcacaca ctgaaaatac tcctgccaca
     5221 attgtaaaag ttgatccacc acgtacaatt tgagctccag aactatctct tgtataaata
     5281 aaaattggtg ttaacacgcc aatgaacata cctgctaagg tagctcctat actacgaaat
     5341 gttgataacg ctgtcctttc ttctgcaaca gaagtgatag ctgatgccat tgaaccgtaa
     5401 ggaatattaa tagatgtata aaaaatactt ccccatagta tatatgttac atacatatat
     5461 ataactttaa tactcataga agctgatgcc attgatgttt gatacattaa aaaacttgct
     5521 aaagcgactg gaccactcat ccgtaaaatc cacggtttaa atttgccagt tttagatggt
     5581 tttgatccat ccacaatacg ccccattgtt atgtctgtaa ctgcatcaat aaatcttgct
     5641 actaaaaata atgttcctac ggtagctccg cttatcccca atacttttgt ataaaaaatc
     5701 attaaaaaag aactagcaaa aatgaaagtg aaatcattcc caaaatctcc aaacatatat
     5761 cctattttgt ctcttagacc aaattttctt tccatataat ttgcgctcct tcctattgtt
     5821 aagcgttttc attctgttga actgccaact tacgtactat actctgaagt ataaagagtt
     5881 agtatttatt ttttacattt tatagtatta tttatttttt ctcctaataa ttctttatct
     5941 cataaaactt atctatatat ttactagcta ttcgagttac ttcttcaaaa tcaccttttt
     6001 caacaggagc caacaaattc ccacctacac caactgcaac aacgcctgct tcaaaccact
     6061 cttctaagtt atctagactt actccgccag taggcattat atttacatat ggtaatggag
     6121 cttgatacgt ttttacaaca gatggaccaa aagcagaacc tggaaatagt ttaatgattt
     6181 ctactccagc ggtaagtgcc tcctttatct cagtaattgt catacaacct ggtaaataag
     6241 gaatttgata taaattacaa atctctgctg tttgtcgatc aaaagatgga cttacgatat
     6301 attctgctcc agccataatt gcaatacgag cagtaaacgc atctaaaact gtaccggctc
     6361 caattattac atctttttga tcagcatatt ttttagcaat ttctttgatt atttcttcag
     6421 cattcggaac tgtaaaagtt agttcaatcc ctttaattcc acccgcagta attgcattta
     6481 ttgctttaat ggcctcatct ttattttttc cacgtacaac cgcaatgact ccagccgctt
     6541 ctaacttact taaaatttct acttttttca tatctatctc cttcttttat ggatacatac
     6601 tctagctagt tgtactaact tacaataaat aaatttaagc ttttattttt tgtattattt
     6661 tctactttcg atttcaaaaa aattaaaata attatacgca ttacgataac aaatattctc
     6721 cactatctga ctaagataca tttcatcatc tggaattcga cctgctttaa cccactcgct
     6781 tagtaattga cataatactc gacggaaata ctcatggcga ggatagctta gaaaacttcg
     6841 agaatcagtc aacatacctg taaaattcga caacaaactt tgttgcgcca tcgtagtcaa
     6901 ctgttgacgc attccttcag ctgtatcatt gaaccaccaa gcacatccca attggatttt
     6961 ttgaatcata cccccttgga agctttgcat tgctgttgct agtggaagcc aatcggttgg
     7021 atttaatgaa tataaaattg tttttggtaa gcacccactc ttttcagcag tgtcgaacaa
     7081 tgctacaaca ttttgtgtca aactagcttg atcccccatc gaatcaccac cgacatctac
     7141 tccaatttgt tcctttaata tacttgaatt atttcttaaa gcatttaaat gcatttgcat
     7201 cgtccaacca tatcttgtat actcagacat caaatgtaat tgaatacctg taacaaattg
     7261 tgcaatttct gataaatgga aatccgtttt tcctgctaaa gcttttatta acagattgtc
     7321 gagtattttt tcatctgcta tttcataaaa ataagtattc aaaccgtgat ctgctaatcg
     7381 acagccaatt tgatgaaaat aatccactcg ttggctcaat gcctgcttca acccattaaa
     7441 gtttttgatt ggtatacccg ctgcttgact aagttttaaa atatagttcg aaaatcctga
     7501 tgatttgata ctccagacct tatctggacg gaaagtaggt aataccttga aagaattatc
     7561 ctgtttagaa agctcagtat gatatcgcaa gtcatctgta ggatcatccg ttgtacaaat
     7621 ggctttcaca tccatcatct tcagcaattc ttttgggcga taggaatccg tttggatttt
     7681 ttcgttggca cgtcgccaaa tttctggtgc attcttctca gaaattgtca aatcaatatc
     7741 aaagtaacgt tgtaattcca aatgagacca ttcaaaaata ggattacctg gcgccttctc
     7801 cactgttttt acaaaagcta aaaatttttc ataatcatta cctgcaccac tgatcaactc
     7861 ttcttctatc ccattagctc gcatcaatcg ccatttataa tgatctccag ctcctttatc
     7921 atttaaccat acctgagtca aattcttgta ttgcttattt tctagcaaac atgctgggtc
     7981 taaatgacaa tgataatcga tgattggttg gtgttctgca aatttatgaa aaagtctttt
     8041 agctatttca ttctctaata aaaagttctt atctaaaaac atatttaagc actcctttcc
     8101 ttcccttagt ttttagaaga gaaaattttt cttctaaata gactccatta tataattatt
     8161 tactcgcccc cttttcaagc gcttcccaaa taccatttaa ataaactgca cctagagctc
     8221 ggtcatataa cccataacca ggtctaccgg tttctcccca aatcatccga ccatgatctg
     8281 gacgaatata gccattaaat ccattatcgt acattgtttt taatatctta tacatatcta
     8341 gagaaccata ttctgattta tgcggcgctt cataaaaatc gagtccatta tcacttaaat
     8401 atttgatatt acgaacatgt gcaaaaggaa tccgatcccg ttgacaaaat tcagatagaa
     8461 tttcatagac atcattatct ggtcgttctg caatacttcc agtacataac gtaataccat
     8521 tagaaggaga atccactaca ttacacaacc attctaaatc gtcacgattt ttaacaatac
     8581 gaggtaatcc gaaaatagaa tatggtggat catctggatg aactgccatt ttaatcccaa
     8641 cttcttcaca tgtcgggata attgctttga gaaaatatgc aaagttctct cgcaatttcg
     8701 cttcatcaac ttctttatag gcttctaata attgcttgat ttgtgataac cgatgaggtt
     8761 cccaacccgg caattcaaaa ttattagtag aattttctac ttttctaata atttcctctg
     8821 gatcagtagg aatatttttt gaaatatagg ctaacgtaga agacccatca tctaaatgat
     8881 aatccaaatc tgattttacc caatcaaaga ctggcataaa attataacag ataacctcaa
     8941 tcccaaattc tgccaaattg cgaatcgtct gtttataatt ctcgatgtat tgatctcgtg
     9001 ttggcaaacc aattttgata tcatcatgaa cattaacact ctcaataact ttcaaggtaa
     9061 ggccattggc ttcgacttca tcttttaaag ctttaattcg ttctctcggc cagacctctc
     9121 cggctggtat atccattaat gttccaacaa ttcctttcat tgctgggatc tgacgaattt
     9181 cttgtagtga gattttttct tcatttgccc cataccatct aaaagtcatt tccatagtta
     9241 ctttcctcca acttctgtta tatatttatt gattgtttta cgaactgctt gaggacctac
     9301 tagcatttcg caaaagattt tttcaatttt ctttcctatc tcaacttgat ataaatctaa
     9361 accaaatatt tccttatttt ctaagatttc ctgtaccgca caatgaatct ttgcaggatc
     9421 ttgttcacct aatttcaata ctgagaattt ttcctgcaac tctaccaata aaggatcagg
     9481 acttggcaca aaattttttc cttgatcatc gactgccaat aaataacgta accaagcagc
     9541 aatagcgata ggaataaatg ttagattttc tgcatttccg tctttttcga catacttttt
     9601 aattgtttca ccatagcgaa tcgctacttt ttgagaagta tctgttgcaa ttcgttgcgg
     9661 tgtatctgga atatagggat taggcaaacg tttattgatt acttcatcaa taaattgttt
     9721 tggatcaata acgcctggac tttcaactac tggtaagcct tctacataac ctatcccttt
     9781 aattaagcca accaaatcat catcttgcat ttctgcctga attgatttat accccaacaa
     9841 acaacctgtt attgctaatg ccgtatggag cggatttaaa catgtcgtaa ccttcatgat
     9901 atctgcttta tcaactgttt ctctatctgt cataatcaca ccagccgctt ccagtttagg
     9961 tcgaccattt ggaaaactat cttcaatgac tagataatgg actttttcag tattggtaaa
    10021 agcagcgata ttggtatttt tcgcagtatg aattatatct aagtcttcga aacccgcctt
    10081 caataaatcc attttgacac tttccgaagg atttggcgta attctatcaa tcatactcca
    10141 aggaaaagtc actttttctt tatcctttaa ataagcaata aatttttcag aaacgtatcc
    10201 ttttttttgc caagcttctg caatcataaa aatgctatct tgaaaacgtt gaccattctg
    10261 tgagaaatta tcagtagaaa ccatcgcaat cggtaactct ccagcattaa acctgctata
    10321 caacaaggat gtcacaatac tcatcgtatg ttgaggattg gtaggtccag attttatatc
    10381 tgcttccact accgaaaaaa attgtccttt agaatctttt aaactgtatc ctttttcagt
    10441 aatagtaaac gttgtaaact gtaatgtagg atcttcaaaa tatttaacca ctctttgata
    10501 gtccgttata ttctttggat tgcaatataa actatcagct gttactgcta aaagtctttt
    10561 tttgagttca ccatcttcac ccataacaat ctccaaaata tcattgttgt agtcatgata
    10621 agctttttct actacttctt catcaaatgt ctcacacaca actactccgg attctaattc
    10681 attttgatct gctaagcatt gagcaatttc tgcatgaaat cctcgataaa gatttcctcc
    10741 accaaaatga agccacactg gatgtttctg accttcttgt ttgaccgctt cgacattaaa
    10801 atacggtagt tgaacttgca ttttgttcaa tccttcgata gaatcttgcc agtctttaat
    10861 caaaaccata tttacccctc catactgata tgttagtttc cgttacaatt gtagtataaa
    10921 atcgaatgta atcgctgtca ataagaaaaa actaaaaata ttcttttttc taccatgtca
    10981 gaattattgt gtcttttttt agtaaaagca caccaatata tattttttag aactaactta
    11041 aattaaagct taatttctct ttgatcttat ttgagtattt tttgtttttt taatagctag
    11101 tatgttactt tgaaatttga tattatataa cgagcataga actaaaatat caaattcaaa
    11161 aatgaaattt tgagagggat taaatatgac aattaaaact agtctccaaa atcaagctta
    11221 tcaatctatt cgtcagcaaa ttatctacgc tgacttagaa cctggaaaaa aagtatcaga
    11281 aaaaaaattg gaagaaacgc tttctatcgg tcgtactcca atacgtgaag cattgattca
    11341 actccgtcat caaggacttg ttgatacaat tcctcaaagt ggaaattata tctcattaat
    11401 cgacttagat ttagcaaaaa atgctcgttt tgtcagagaa catttagaac gacaaattat
    11461 gctagagtgt tgtgccaaga tgaataatca aaagaaacaa gttttagaaa ctataattgc
    11521 agaacaagaa aaagctgtta gtcaaaagaa tgctcgtagt ttttttcata atgataattt
    11581 atttcataaa gcttgttttg aaattgcaga acgggaagaa gtatggcaat ggttggaaga
    11641 taataatacg cacttggaac gatttcgttg gttacgagta aaaacggaag gattaaaatg
    11701 ggaaaaaatt atggaacaac atcatcaact atttaaagcc ttgaccatgc aagaccctga
    11761 agaagctaac ttcttaacat ccttgcatct tcatttaatg cttgatgaac aaaatgttgt
    11821 attgactact cacccaaaat attttaaaaa tatataactg ctaaatttct tttttttaat
    11881 ttatttacat ttctgattac tacagaaaac taactacttt ttttatgtaa agttctgtca
    11941 aatatagtca ctttatatta ctttctcttg agtaaaaaaa catgtgaggt cgtaaacaaa
    12001 gattgaacta tctacattca tactctataa tctgtttatt tgcagactga taatagcaaa
    12061 agaaaactta taatcaaata atatcaacat ttgatcacat aatttgagat tgaatgctga
    12121 tttacctatg ttttacgtaa tggtctttag ttatttttgc tcataaaatg cataaaacac
    12181 gcgtttttat gtgtgcttga aatttcttgg ctctataatt tctaagactg ataaaaatat
    12241 tctatcggtt ctttttgcaa ataaaaaaac atatagaagc tccattagat tgaatcacca
    12301 caacacaatc aaaggagcat tccgtatgct tgattttcat tctataaaac tacttttagg
    12361 aatcaaagaa aatcacaacg gttcaaacta aaagcaagaa tatccgaagt accattgttt
    12421 catcgatcat gagaaaagca ttcacctctg tctgcagatg ttcttatatc gctctgtcag
    12481 tcctattaat aaagtactgt tttgttttca aaaaaactat tacgagtact ttttaagaga
    12541 atgccttgca aatattcatc acacaccgtt atataagtca aatagtttct ttcagtcgtc
    12601 aagaaaagat tctcctgtcc ttggcattat cagttaagta gcaacttaat tctcggactt
    12661 gttctactct agcaaaattt attttcatca acttacgtcc aaatgttttt ttgaataatt
    12721 acttcatcgc gggtctggaa gacttgcctc attcgataag tcactagctt ttggcgtcct
    12781 tcaggatttc tagtatacac caactgacat ttgggcagcg acgattgatt taattcgttt
    12841 agaaaaaggt tagcgatttg cttatttcgg ccaaagcgtt ctggtaccgt cagaactatc
    12901 tttttccctt cgattaaata ccgcggattc tccagcggtg ctaaaagctc cctcatagct
    12961 tcagtaaaaa gctgtccttc tcgatagcca gcattctcaa gatagcatct aaattcctgc
    13021 tgatcagccg ctacgattac ttttctatca ctaacttcac caaggcgctg caacgtcgtt
    13081 tgaacgcata tggccaagct ttttttgcgg gcacattcct tttcccatag cttttgtcgc
    13141 ctacgaattt ggaaatattc cagcaagtta cctttaaata tagctgacca gaagcttaga
    13201 ggctctatat tttttactac tatcggcaat gaagtttctt gtttttgctt tacctgatcg
    13261 actttcctaa cgacgattgg catagatagg tgccagccat cagctaaagc agtactccat
    13321 tgttctttca accgacttcg ttcacaagaa aaatagaaca tcgactgagt ataagtttcc
    13381 atcgtggtgg tcgtatattc cagtggtaaa gcaaagcgat cgccaccact ctcaattttt
    13441 ggtgaagatt caaaggtcag acctgaaata ttgcccaaac gccgtagcat tcttttcaaa
    13501 tcaggctgtt cagacagagg aactcccggt aatccaatcg ctaaatgcca aatgttcgtt
    13561 actttatttt gtgcacctag gcgtaagccc cgccctcgta gttgttgtgt ttgcacaaaa
    13621 gtggaagctt gattagccaa gatcaacaga ttaattgcag gacaatccca gccttctcct
    13681 agcaaactaa cggttcctac caaaatattc aacttctttt cggttaatag gcgagtaact
    13741 gcttgcatca atgcttggcg gtttttgtca tccaccgtca aataaaaata acccgacata
    13801 taaggatgtt cattcatacc cgtcgctagt tctgccagct caacagttac tagcattttc
    13861 ggtaacagta agaactctcc acagatggca ccgatttgct gagtgttacc taatagacga
    13921 acaacttcat caaacaaagg aaccacgcca taagctaagg taccatcact gcctgctaat
    13981 acctccttgc aaatacgatc catcaaaatg accgctgtca gttcttgctt cagctgaact
    14041 tcttctttta acaaaatttg acaaactgct tgctttttct caggaatcgc ctgactacca
    14101 acagtgtctg gatactctgg aaacaaatgc agcgactcat caaacaatca tccagctttt
    14161 ttcaattgat aaaaaatagg caattccggt tgatcttcaa acatctgctg ataaatagtg
    14221 atcagctctt ttttacctaa agatggaact gaagcttttt taccttgctg cttagaactg
    14281 cctaatagct gccaatgttc gggtatcaat tcatagttat gatgattcaa gtaaattaat
    14341 gcactagtga acagctcaaa attttcataa atttccttcg aatgctcaag cggatgctga
    14401 acaaattcta aagaaagcaa ataatccacc gtctcttggc tttccattaa cgcttccacc
    14461 agctcatttt gtttctctaa aaaggtaaaa taatgctgag tagtttcctg attagccgga
    14521 atcaaatata gaaaatcctg atgaggacat agaacctcct cctttaccaa ctcgggaatg
    14581 ctaatttctt cgtcaatcag gccattttaa ctcaatatat tgcttccact ccgtctgaga
    14641 gctatcaatc ggaggcgtgg ctgtcaagga aatcgtcaac agattcggtt gttttttctt
    14701 caagtcaatc aacaattcac cccagctttt ttttaaatga tgagcttcat caaaaaacta
    14761 acagctccag ctgatcacca taagttggct cctccttcag tcggttgtaa atggtctgat
    14821 acgtttccac tgtaatatga ctaggatgtt caatcgcccc agaaagatag caactatctg
    14881 ctggatttac taagaaatat tcctcgattg ttgttaacca ctgtttcttc aatattaggg
    14941 aaggaaccaa aattaaggtt ttacaatcca aacgtcggat catctcaatt cctaaaaagg
    15001 tcttgccagc ccctggtgga gccactagat ggactgaatt gttatttaaa taagtagcaa
    15061 agttagtcaa tatacgcttc tgatagctac gccactgaat tttttcatca gatagccaca
    15121 tcccaggtcc ccctctcacg tattttctct atcataatct aaatgaatac agcaaaaaaa
    15181 agatttcttc aagaatatgt gtttttatag taatatttta cttactctaa actcaaatga
    15241 aaaggtaaaa aaactggtag tacaatcttt taaattctca ttgttttgta cgttctttac
    15301 tatcaaaaaa acaaaaaaaa cgcgtttcta cgcgtgacca aaatttttag ctctataatt
    15361 tctaagactg atagaaaatc attctgtttt tgaagtcttt tttcataaat actacttgat
    15421 ttattgtgta tattgtataa aagatt
//
LOCUS       DAZUKR010000059        15026 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000059 DAZUKR010000000
VERSION     DAZUKR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.059"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..2632
                     /gene="adhE"
                     /locus_tag="ACG89C_001618"
                     /gene_synonym="adhC"
     CDS             35..2632
                     /gene="adhE"
                     /locus_tag="ACG89C_001618"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4900160.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2861..3967
                     /locus_tag="ACG89C_001619"
     CDS             2861..3967
                     /locus_tag="ACG89C_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4900161.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3984..5012
                     /locus_tag="ACG89C_001620"
     CDS             3984..5012
                     /locus_tag="ACG89C_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS4900162.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5018..5497
                     /locus_tag="ACG89C_001621"
     CDS             5018..5497
                     /locus_tag="ACG89C_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4900163.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5513..6808
                     /locus_tag="ACG89C_001622"
     CDS             5513..6808
                     /locus_tag="ACG89C_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4900164.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6819..7643
                     /locus_tag="ACG89C_001623"
     CDS             6819..7643
                     /locus_tag="ACG89C_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4900165.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7808..8707
                     /locus_tag="ACG89C_001624"
     CDS             7808..8707
                     /locus_tag="ACG89C_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900166.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8833..9702)
                     /locus_tag="ACG89C_001625"
     CDS             complement(8833..9702)
                     /locus_tag="ACG89C_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4900167.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9855..11315
                     /gene="rhaB"
                     /locus_tag="ACG89C_001626"
     CDS             9855..11315
                     /gene="rhaB"
                     /locus_tag="ACG89C_001626"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4900168.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11312..12568
                     /gene="rhaA"
                     /locus_tag="ACG89C_001627"
     CDS             11312..12568
                     /gene="rhaA"
                     /locus_tag="ACG89C_001627"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4900169.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12582..13427
                     /gene="rhaD"
                     /locus_tag="ACG89C_001628"
     CDS             12582..13427
                     /gene="rhaD"
                     /locus_tag="ACG89C_001628"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4900170.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13429..13740
                     /gene="rhaM"
                     /locus_tag="ACG89C_001629"
     CDS             13429..13740
                     /gene="rhaM"
                     /locus_tag="ACG89C_001629"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4900171.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13822..14973
                     /gene="fucO"
                     /locus_tag="ACG89C_001630"
     CDS             13822..14973
                     /gene="fucO"
                     /locus_tag="ACG89C_001630"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4900172.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
BASE COUNT         4822 a         2587 c         3410 g         4207 t
ORIGIN      
        1 taacaaataa ccatgaccca ttaggaggat aatgatggct aaagtaatag aaaaagaaaa
       61 aacgaagaca attgacgtac aagcaatgat tgatgatttg gcagaaaaag caaatgcagc
      121 attgaaagaa atggaaagct ttgatcaaga aaaagtagac catatcgtgc atgaaatggc
      181 aatggcagct ttggatcaac atatgccatt agcgaagatg gctgtagagg aaacaggccg
      241 cggtgtttac gaagataaag caatcaaaaa tatgtatgcg tccgaatata tttggcataa
      301 tatcaaacat gataaaacag ttggtgtgat caatgaagat gtccaaaaag ggttgattga
      361 aattgctgaa ccagtcggtg tggtttgcgg agtaactcca acaacgaatc cgacttccac
      421 aacgattttc aaatcaatga tcgcgttgaa aacaagaaat ccaatcgttt ttgctttcca
      481 tccaagtgcc caaaaatctt ctgcagaagc agcacgagtg gtacgtgatg ctgctatcgc
      541 tgccggagct cctgaaaatt gtatccaatg gatcgaacat ccttcgatcg aagcgacttc
      601 tatgctaatg aaccatccag gtatcgcgat cgtcttagcg acaggtggag ctggaatggt
      661 caagtctgct tactctacag gaaaaccagc tttaggtgta ggtccaggta acgtaccagc
      721 ttatattgaa aaaacagcaa aaattaaacg tgcagtgaac gatttgatcg tttcgaaaac
      781 atttgataat ggaatgattt gtgcttctga acaagctgtg atcgtggata aagaaatcta
      841 tgcagcagta aaagcagaat tccaagcaca ccaagtttat atcgtaaaac cagatgaatt
      901 gcaaaaatta gaagatgcag tgatgaacga aggaaaatat gcggtcaatc catcaatcgt
      961 tggacattct gcgatggaaa tcgcaaaatt ggcaggaatc agtgtaccaa aaggaacgaa
     1021 aatgctgatt gctgaattgg aaggcgtagg cccagattat ccgctatcaa gagaaaaact
     1081 ttctccagtt cttgccatga tcaaagctaa caacacggac catgcgtttg atttgtgtga
     1141 aggtatgttg aatcttggag gattaggaca tacagcagtt atccattcag aaaacgaaga
     1201 gttgcatgtc aaattcggtc ttcgaatgaa agcttgtcgt attttagtga atacaccatc
     1261 tgcagaaggc ggcatcggtg atatctataa cgaaatgatt ccgtcattga ctttgggatg
     1321 cggttcttat ggtaagaact ctgtttctcg taacgtctct gctgtaaact taatcaatgt
     1381 caaaactgta gcgaaacgga gaaataacat gcaatggttc aaattaccgc caaaaatctt
     1441 ctttgaaaag aactctctat tgtacttaga gaaaatggaa aacgttgaac gtgtcatgat
     1501 cgtttgtgac cctggtatgg tccaattcgg ttactgcgat acagttcgtg aagtattggc
     1561 acgacgtaaa aatgatgtga agatcgaagt cttctcagat gttgaaccga acccatcgac
     1621 aaataccgta tacgcaggaa cgaaactgat ggcagacttc aagccagata cagtaatcgc
     1681 tcttggaggc ggttctgcaa tggacgcagc taagggaatg tggatgttct atgaacaccc
     1741 agacacatct ttctttggtg caaaacaaaa attcttggat atccgtaaac ggacatataa
     1801 gatcgacaaa ccagaaaaaa cacaattcgt atgtatcccg acaacatcag ggactggttc
     1861 tgaagtaacg ccatttgcgg tcatcactga cagtgaaaca catgtgaaat atccgttagc
     1921 agattatgca ttgacgccag atgtcgcaat cgtcgatcct caattcgtca tgtcagttcc
     1981 agcatctgtc acagcagata ccgggatgga tgtgttaaca catgcaatcg aatcctatgt
     2041 ttcagtcatg gcttcagatt atacacgtgg attgagccta caagcaatca agttagtctt
     2101 tgactattta gaaaaatcag taaaaacacc tgatatggaa tcacgtgaaa aaatgcacaa
     2161 cgcttctaca atggctggta tggcgtttgc taacgcattc ctaggtattt gtcactcagt
     2221 cgcacataaa atcggcgggg aatacgggat ccctcatgga cggacaaatg cgattctttt
     2281 accgcatatt atccgctata atgcaaaaga tccacaaaaa catgcgatgt tccctaaata
     2341 tgactatttc cgtgcagata cagattacgc agatatcgca aaattcttag gtctgaaagg
     2401 cgaaacaaca gaggaattag tcgaagcttt agcaacggct gtttatgaat taggaaaatc
     2461 tgtcggcatc aacatgagtc taaaagctca aggagtcaca caagaaacac tagatacaac
     2521 cgtagaccgt atggctgaat tagcttacga agaccaatgt acgacagcta atccgaaaga
     2581 accattgatc tctgaattga aacagatcat cattgatgct tacaacggat aagcaaaaga
     2641 aagcgtagaa aaaagataag aaaatagatg aaaaagaact tggaaaacgt tgtttctcca
     2701 agttcttttt ttaatttcca agtaactgaa agttactttt gttatttaac atttaggtca
     2761 attttagaca gtttttgttc aaaaagcgat atttatagtg gacggtatta ctgctatatt
     2821 aaggatataa aatgaaagcg tttaaaaagg tggcgtaaag atgattatta aagaagagat
     2881 ttttgtacct aaagaattag tgaaagaaaa aattgacctg ctcgttgaaa acctgacaac
     2941 gatcaaagac gataatggtg aatttttgtt ggactttgat ggactgaaag tagatgacaa
     3001 aagctggaca gtgtggaatt ggccacaagg tgtcggtcta tatggtattt ataaaaatta
     3061 ccagatgaca aaaaatcctc gcgcttatca agtggtcaat gaatggtttg aggatcgtat
     3121 ggaagaaggc gctccgccca aaaacgtcaa tacaatggca ccgctattga ctatggctta
     3181 cttgtatgaa gacacgaaag attcacgcta tttgccttat ttagaacaat gggcagaatg
     3241 ggtcatgaat gacatgcctc ggacaaatga agacgggtta cagcatgcca catatggacc
     3301 ggaaaataag aatcagttgt gggacgatac attgatgatg acagtgcttc ctttggcaaa
     3361 aatcgggaaa ctgttgaatc gcccagaata tttagaagaa gctcgttacc agtttatgat
     3421 ccatatcaaa tacttgatgg ataaacgaac aggattgtgg tatcacggct ggacgtttga
     3481 aggaaatcat aactatgccg aagcattttg ggctcgtgga aactgctggt tgacgattgc
     3541 tattccagag atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt
     3601 gatcgaaaca cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt
     3661 atggcatacg ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt
     3721 tgcttacggt attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt
     3781 cgcttatcgt gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa
     3841 cgtttcaatc gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac
     3901 agcaatgcct tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta
     3961 ttgttaaaag aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc
     4021 ttagcctagg tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg
     4081 cagatgcaga tgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagc
     4141 cagtaattga tgcaatgaat gaatttgggc gtttagtaaa agaaaaaaca gatggttccg
     4201 tacagatcca gtatttccca gatggacaac tgggtggaga acgtgaactg atcgaattga
     4261 cacagtcagg agcagtagat ttcacaaaag taagtgcctc agcgttggaa agtttctcta
     4321 aagattattc gattttttca gttccttatc tattcaatga tgaagaacat ttctttaagg
     4381 tgatggataa tgaagaaata atggagccaa tctaccaatc gaccaaaaat ttaggattta
     4441 ccggattgac ctattatgat tctggtcagc gcagctttta tatggtagac ggtccgatcc
     4501 atacgccgga agatttgaag ggaaagaaga tccgtgtgat gcaaagcgaa accgctatca
     4561 aaatggtaga gcttttaggc ggttcgcccg tacctatggg aagtgatgaa gtgtacacgt
     4621 ctcttcaatc caatttgatc aacggatcag aaaacaatga atttgtcttg tatacagctg
     4681 gacatggtgg tgtggctaaa tactattcct atgatgagca tacacgtgtg ccagatatca
     4741 tcatcatgaa tgatgccatc aaagaacagc taactgatga gcaggaaaaa gccattgaag
     4801 aagcagcgaa agaatcaacg gtttttgaaa ttgaagcttt tgcaaaagca attgaagaag
     4861 aaaaagcttt agcacaagaa gaacatggag tccaattcaa tgatgtagat aatacaccgt
     4921 ttcgagaggc agttgcccca cttcatgata tattcaaaaa cgatcctacc tacaaagacc
     4981 tttatcaaaa gatccaagag caaggagcgt agctgtgatg aaaatgattt taaacaaggt
     5041 attagagatc ctgggagctg ttttgcttgt tgggatggtc gctgttgttt tatggcaagt
     5101 attttccaga acggttttag gaaatccaaa tacggtcacg gaagagcttg ttcgattcgg
     5161 tcttgtctgg tttgccatgt tgtcttccgc gtatgttgtg ggacaaaaag cacatttagc
     5221 cgtaacggtt ttaagtgaaa aattaactgg caaaaaaagt aaaattttag aagttgttgt
     5281 tcaaagttta tttctggtat ttgctgcgat gatcatggtg tatggcggat ggaatgctgt
     5341 cacattgaca atggggcaga tctcgccttc tttaagtatt cctatgggat atgtttatct
     5401 gtcagtacct gtatcggggg tactcatcat tatctatagc ctgatcaatc taatcgatac
     5461 attaagcaac aagccagcaa ttcaagaaaa aatataaaaa tggaggaata aaatggcaat
     5521 agaagccgga ttagtgttat tttttgtatt tgttgtgttg cttgtattag ggatgccgat
     5581 tgctataagt attgcattct catcgatgat gactttattg ttagttatac cgtttgatgt
     5641 ttctgctttt agttcagctc aaaaaatggt gggaagtatc aatagttttt cgctgatcgc
     5701 tattccgttt ttcgttctct cgggcattat catgaataat ggagggatag ctaagaaact
     5761 agtggatttt gcgatgttgt tcgtcgggcg cgtgccgggg gctttggctc atacgaatgt
     5821 gttagggaat gctttatttg gttccatctc tagctcggcc attgccgctt ctaccgcgat
     5881 tggcggagtg ttgatccctc agcaagtgga agaaggatat gaccggaagt ttgcgacagc
     5941 ggtcaatatc gcgtcagctc cgacaggtat ggtcattcct ccaagtactg cttttatcat
     6001 gtattcactt gttgctggtg gtgcttcgat ctctgctttg tttatgggag gttatctcgt
     6061 aggcgctttg tgggcgctag gaatcatgct agtagcattc atcgttgcta aaaagaataa
     6121 ataccctgtt gtcgaaaaag gacagatggc tcatgctgga aaaataataa aagaggcgat
     6181 tcctagcctt ctattgattg tcatcattat cggtggtatc ttgacgggga tatttacagc
     6241 agtagaagct tcagcgatag ctgtcattta ctccctgttg attgctatgt tttactataa
     6301 aacggtaaaa ctaaaagata tacctaatat gttgtttcaa tcagtagcaa tgtcagggac
     6361 gatcatgttc ttattggcta cttcttccat gatgtctttc gccatggcgt ttaccggtat
     6421 tcctcaggca attagttcgt tgattatggg cgtaacggat aataaattta ttattctgtt
     6481 attagtcaat cttgtgttat tactggtggg tatgtttatg gatgtggcgc ctgctatctt
     6541 gatttttaca ccaatatttt taccaatcgt cacaagtgta ggggttgatc ctgtacatta
     6601 cgggttgttt tcaatcatga atctgtgtgt aggctccatc acgccgccag taggtacagg
     6661 actatatgtc ggtgcaagtg taggaggcgt aaaagcagag gcaatgctga aaccgcttct
     6721 tcctttttat ggggttatct tagtcgtctt gtttttgatt acttacttcc cgcaaatcgt
     6781 catgtggcta cccaatatgc tgagttagga gaatagttat gttgaacatt acaattagag
     6841 gacatgatct ttccgaagtc caaacgatag aagatcttgc agaaaaaaca aaagaacaag
     6901 ggattcatac actgcagctc gccttaggtc tttcgtttcc tgaaatacct tctggcgcaa
     6961 atgagatcaa ttctgggatg ggaaattatg tcaaacgagt tttagaaaag caagaagtga
     7021 ccgtaggaat tttaagttgc tatatcaata tgattcatcc agatttaacg atacgacaag
     7081 agttgctgac aaagttcgag cagtatcttc gctatgcttc ttcctttggt gcgtcgatgg
     7141 ttgcttcgga aactggatgt gttttgccgg aaatccaata tacggaagaa aatttcacag
     7201 atgaagcatt tgctgaagct gtgtccgtta tccgtcgctt ggtaaaagct ggagaaaaat
     7261 accaaatgat ggtggggata gaaccaggtt tgaatcatcc ggtgtattct ttagcaaggg
     7321 tagagcagct gatccaagca gtagattcgg actatctagg aatcatttta gatccgacca
     7381 atttgatcac aagtagtgat tatcaagaac aagtccaatt ggtagaagaa gcttttgaac
     7441 gttttggtga aaagatatgt gcggttcatt tgaaagattt tcgtgtcgaa caggaaaaaa
     7501 tcgtgccggt aaatttggga gacggtgtga tcgagtacac aaaaatcaaa gaaatcatca
     7561 tgaagaatcg tccgtacctg tatgtggttc ttgaagaaac aaaagatgat gggattcgtt
     7621 atggaagaag tcttcttgaa taatgtaaag aagacgttgg ttaactaata tttagaatgg
     7681 agggggcggg gctagaagat gccacgtctc ttctttttta taagagtata taaaagcagc
     7741 tctttttgat tacaaaaaac gtggtataat aaaattgtgc ataagtgaac aaaaggaggt
     7801 gcggcacatg ctccaaaaga aaatcagcgg tgaacaagtc aatacgatgg tttctgatca
     7861 gtatgaaatc tatcatgtca aagatgtcgt cagccaaaat aaaacgatct atcatcatca
     7921 tgatttctat gagatccatg ctacattaaa gggagaagcg actttttatt tgaacggacg
     7981 acaatttacg atcaaagcag gaagtgtgct gctcatccat tccaatgatt tgcatcgtat
     8041 cgtccgccaa tcttcagatg tattcgagcg tgtctatatc tttgtcaccc cttcttttct
     8101 agaaagtcgt tccacgccat ggtctaatct atcggcttgt tttcaaccaa tgggagagaa
     8161 gaggagtcgt gtattgcaaa tagaacagga agtattgaag caaaaattat catttatcga
     8221 ccatcctcct gataaaaaaa tttacggtga agacataaaa tatgaacaag ctctggtaga
     8281 atacttgatt tttctcaatc aagttgtaca aaaagaagag agtgcgcaag aacagaatgc
     8341 tgtagttcct aatgagcgtg tggaacaaat gttacaatat atctcaaaaa atttatctca
     8401 tccgctttct ttagaggaaa tggaaaagca gttttttgtc agcaaatacc atatcacacg
     8461 agaatttaaa aagtacactg gatttacgtt tcatcagtat gtaatgaaga aaaagctgat
     8521 ctatgccaag cacttgttgc gagaataccg cagctctagt gctgtatata gtaaaagtgg
     8581 tttttcttct tatccgcatt ttctgaaagc attcaagaga gagttcggca taacgcctaa
     8641 agaatttttg aaacgtgata cacagaaaca gttggttcat tatgaacatt ttgaagatga
     8701 acaatgagaa tgatcactaa aaaataaccg cagtagctgc tgttgtttac atagaagcag
     8761 aaactgcggt tattttattt ttatctatat atttcaaaat attttgaaaa aatgacgtac
     8821 atattatatg aatcagctga atttacgaaa gtctctcggc gatagtcctg tttttgcttt
     8881 gaacaggcgg ctaaagtaag caaaattctc aatccctatt tcttcggaaa tcattcgaac
     8941 cgtaaattct gtataccgaa gaaggtcttt agcgtaagaa agttttttgc tgatcaaata
     9001 atcgattggt ggagaaccga taaatttgga aaaatctttg ctcagctgat atttattcaa
     9061 taagaattgt tgttctaatt gatcaagtgt aatcgtttct ttgaaatgtt cgtctaagta
     9121 ttgcttcagt ttgtaaacgt aggcgggaat atcctcttct tcgaaatcta gctgatactt
     9181 ttgcaaaatc agttcattca gcaactcgtg gatagctaca gaggactgga aatcggtacg
     9241 ggcattcgtg ccttgttgca tgtggatcaa ttctgtgatc aaatggtgga tcttgttttc
     9301 tgatggatct cctgttgaat ggaaggtgtt atggccgtct tttgtgaact cctgataaaa
     9361 tgattggacg ttctgaccat tcaaatggat ccaatccatt tcccatggtt cttcgctgat
     9421 cgtttgataa cgctgatatt cttggcagtc aataaaaaat aagtctccag cttttagctg
     9481 ttgctgatta tcattatatg taagcgaacc tttgccagac aaagtaaact ttatcaagta
     9541 agaaggtaaa tgtgctcgtt cagtatagta agggattggt gctttgaagt aaccgatttc
     9601 ttggatataa aaaaataaat tcctgcttct ttgtgtaggc gtcaagatca ctctttttga
     9661 gtcttccgac catgagtggt tggctttttc ccagtaatcc atgtaaacac tcccaacttg
     9721 tcaagataat gcaattatat accaaaatag tgtgaagcaa aatataagat tctgtattat
     9781 gatagcattg tcaataaatg ataacgcttt attaataaag tgctaatcaa caataaagta
     9841 aaagaggtgg agaaatgaat ccaacatttg ttgccatcga tatcggtgca tcaagcggac
     9901 gactaatgaa aagtacgagg gatgcaaaag gttctttgat cttggaagaa gtacaccggt
     9961 tcaaaaacgg ctttcggaac aaagacggtc acgatcgctg gaagatcgat tatcttatca
    10021 aagagatttt gattggattt gaaaaattaa agcagacagg gattgatcgc ctttttgcgg
    10081 ggatcgatac atgggcagtc gattactgtc tgattgatga acaaggggat cggataaatg
    10141 atccgattgc ttatcgggac aagcgtacca atggagcagt agaacagttt tctgaaatct
    10201 attctctaga taagttgtat gagcagacag ggattcagat ccagcctttc aataccttgt
    10261 tccaattgtt tgctgaagaa aaagaaaatt tggcaaaagc acataaattg ttactgatac
    10321 ctgattattt agggtatgtc tttactggta acatggtgac ggagaaaact aatgcatcta
    10381 ctatgcagct gctgaatgca gggacaaaag aatgggaaat agaacttctt gaaaaaatcg
    10441 gtgtctcgaa ggaattattt ccaccattga cagagccggg aacgatacta ggagatctgc
    10501 aaaaagaaca attttcaaaa tatgatttgc cagatgtgac ctttatcaca gtagcaagtc
    10561 atgatacggc gtcagctgta ttaggaacgc caggtcaagg agaacgttgg ggttatatca
    10621 gcagtgggac atggtcgctt ttagggattg aaacaaagat cgccaatgtt tctcgcaatg
    10681 ctttttctga aaattatacg aatgaatggg gtgcccataa cacggtgcgt tttttgaaaa
    10741 acatcatggg gatgtggttg atccaggaag ttgcccgaat gcaagagtac gagtacagtt
    10801 atgcggaatt ggcagagctt gctgcaaaag aaaaaccgct gcagcaagtg atggaaatca
    10861 atgaccctcg atttttgaat cccccaaata tgatagaaga gattcagcaa tactgttttg
    10921 aaacagagca gccagtccct gtctcatcgg gagctatcgc tcgttgtgtg tatgataatc
    10981 ttgctctttg ttacgcacaa gagctgatca aagtagaaca cttaactgga acgaaattgg
    11041 aaaagctgca catcgtaggg ggcggatcga ataatcgtct actgaatcaa ttgacagcag
    11101 attatgccgg aatcattgta gaagcaggac ctagtgaagc cacagcaatc gggaatgttg
    11161 ccatgcaaat gctcagtaca ggtcagtttt cgaatataga acaagcaaga aatgtaatca
    11221 aacagtcgtt tccatgtgaa actttttacc cagatgaaaa taagcaaaac acgatggaat
    11281 ggtacgtgaa taaaatcaaa ggagtaatcg tatgaacacg attgaaagaa attacgaaca
    11341 agcaaaagaa aaatatgcaa ctatcggtgt agatacagat gctgtactag aaaagataca
    11401 ggacatcaaa atttctatgc attgctggca aggagatgat gtcaaaggtt tcttgacacc
    11461 ggatggcgaa ttgaccggtg gaatcatggc gacagggaat tttccaggag ccgcacgaac
    11521 acctgaagag cttagacaag atctggagaa ggcttattcg ttgataccgg gcaaacacaa
    11581 attgaacctt catgctatct atttggatac agaagaatct gtcgatctaa atgaaattga
    11641 gccgaaacat tttgaaaaat gggtagagtg ggcaaaaaaa gaagaaatcg gtcttgactt
    11701 caatccgact ttcttttcgc atccaatgat gaaagacggc tttacattgg ctcatccaga
    11761 taaagaagtt cgtgaatact ggatcgaaca cgggaaaaga agccgcaaaa tcgcggagta
    11821 ctttgggaaa gaaactgggc aaacaagtat caataacttt tgggtacctg atgggatgaa
    11881 agataatccg attgatcgtt ttaccccacg ggaacgttta atggaatcac tcgatgaaat
    11941 ttttacagaa gagttagatg aacggtatgt acaagatgca gtagaaagca aactatttgg
    12001 tttaggcgct gaagcttata cagtcggttc ccatgaattt tatatgggtt acgggttgac
    12061 tcgtgacaaa cttgtttgtc tagatgctgg acattttcat ccaacagaag tgatttccaa
    12121 taaattatct tcattagctt tattcagcaa aggaatcatg ttgcatgttt cccgtccagt
    12181 gcgttgggat tcagaccatg tggttttgat ggatgatgaa ttgcaggaaa tcgctaaaga
    12241 attagtccgt aacgaattgt tggaaaaaac aaatatcggt ctggatttct ttgatgcaac
    12301 gatcaaccgt atcgctgctt gggtcatcgg tacgagaaat acacaaaaag cactgctgaa
    12361 agctatgctg gaaccagttg agcgattgaa agaaatggag ctggcttttg actttacttc
    12421 aagaatggct tatacggaag aattgaaaga tttcccgtat gccgatgtat ggaattattt
    12481 ctgttacaaa aatcaagttc cagttggttt ggattggctg gaagaagtcc aggaatatca
    12541 aaaagacgtg ttggaattaa gaaaatagga gtgtggctat attgaaaaaa atagatattt
    12601 tacaagcgcc ttatgtgaaa gaaatgatcg aaacgacgtc gaatctttat cgcttgggat
    12661 gggatgaaag aaatggcggg aatatttctt atttactaaa agaagaagag atcacaccgt
    12721 tcttggatcc tgaaaaagtg atccgccgca tctcgatgat ttttgatgcc agccgtttag
    12781 caggatgcta ttttattgta actggttcag gaagatattt taaaaatgta gcgagcgaac
    12841 ctgccgagaa tcttggaatc gttcgagttg ctgaagatgg caaaacactg gaacttttgt
    12901 gggggctgga aaacgaatgc cttcctacaa gcgagttgcc aagtcatttc atgagtcata
    12961 tcgcccgttt agaagtagac ccagataatc ggatcgttat gcattgccat gctagtcatc
    13021 tattagcgat gagttttacg catgaattag acgaacgttc ctttagtcgt acgctatggc
    13081 aaatgtgtac ggaatgctta gttgtttttc cagaaggaat ttccatcatt ccgtggatgg
    13141 ttccgggaac aaatgagatt ggggaagcaa cagctgaaaa aatgaaagaa actcgattgg
    13201 ttttatggcc acaacatgga atctacgggt caggaaaaga catggatgaa gtctttggtt
    13261 tgatcgagac agcagaaaag gcagcagaag tctatacgta cgtcaaagcg caaggaccaa
    13321 tcttgcaaac aatcacggac gaaaacctgt ggcatttagc agatgcgttt ggtgtcactc
    13381 caaaagcagg ttatttggag gaagttcata cgaaagcagg tgtctgacat ggtgaaaaaa
    13441 gctttttgca tgaaagttta cccagaaaaa caagaagaat acaaaaaaag acatgatgaa
    13501 ctttggccag aaatgcgtac agcacttcgt gaacacgggg tcatcgacta ttcgatctat
    13561 cttgatccga gtacaagttt gttatttggt tacttagtga tcgaagatga aaagttatgg
    13621 gaaaaaatgg caaatacatc tatcaatcaa aaatggtgga agtacatgga agatatcatg
    13681 gagacaaatc cagatgcctc tccagtcagt tgggaattga tgccagtctt tgaactgtag
    13741 caacaagcaa ctgggcaacc ggttgcttgg ggttctttcg tttttattgt gaaaaataat
    13801 acaaattagg aggaatagaa aatgagtaat cgaatgattt taaatgaaac atcgtatttt
    13861 ggaaaaggag cgattgagtc gatcgtccct gaatttcagc agcgaggatt cggcaaagca
    13921 gctgtcatta ctgacaaagg attgatcgag cacggtatag caacaaaagt aacagaactt
    13981 ctagataaag cagcaatcgc ttatgcttta tacgacggga tcgtacctaa tccaacgatc
    14041 caaaatgtaa aagatggagt ggcttttgtt aaagaggcag aggcagactg tttgatcgca
    14101 atcggtggag gttcgccaat cgatacggca aaagccattg ggattatttt gacgaatcca
    14161 gagttttcag acgtcattag tttagaaggt gttgctgata cgaaaaatcc atgtttacct
    14221 atattagcta taccaacgac ttctggaaca gcagcagaag taacgatcaa ctacgtcatc
    14281 acagatgaag taaatcaccg aaaatttgtt tgtgttgatc ctcatgatat cccaatcgtc
    14341 gcgttcatcg acagcgatat gatgatggga atgccgaaaa aattggctgc atcaacaggt
    14401 atggatgcta tgacgcatgc tattgaaggg tttatcacaa aaggcgcttg ggaaatgact
    14461 gatatgctgc atttaaaagc aatcgaaatc atcggccatt ctttggaagc ttctgtagat
    14521 ggcgatcaaa atgggcgaga aaaaatggca ttaggacagt atattgcggg aatgggcttc
    14581 tcaaatgtcg gtttaggact ggtacacggg atggctcacc cgttaagtgc atggtacaac
    14641 attcctcatg gcgtagcgtg tgcagcactt ttgccaactg tcatgaaata taacaaagaa
    14701 tatactggag aaaagtaccg tgagatcgct cttgttctag ggattaaagg agcagctgaa
    14761 atgtctttag aagatgtaag agaagcggct tgtggtgaga tcgatcggtt gagcaaagcg
    14821 gttggaatcc cagaaaccat ttctgaatta ggtgtaaaag aagcagatat tccagcaatt
    14881 gcagaagatg ctttgcgaga tgtatgcaca ccaggaaatc caagagaaac gacagtagaa
    14941 gaaatcattg ctttatatca aagtttgatg taaaaacgaa gcggatcttc aacattaaat
    15001 tgcctgtaaa gagaaatggt agttta
//
LOCUS       DAZUKR010000060        14815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000060 DAZUKR010000000
VERSION     DAZUKR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.060"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG89C_001631"
     CDS             complement(296..1024)
                     /locus_tag="ACG89C_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900173.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG89C_001632"
     CDS             complement(1002..2576)
                     /locus_tag="ACG89C_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4900174.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG89C_001633"
     CDS             2805..3743
                     /locus_tag="ACG89C_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4900175.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG89C_001634"
     CDS             3755..4666
                     /locus_tag="ACG89C_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4900176.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG89C_001635"
     CDS             4690..6162
                     /locus_tag="ACG89C_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4900177.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG89C_001636"
     CDS             6213..6824
                     /locus_tag="ACG89C_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4900178.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG89C_001637"
     CDS             6854..8767
                     /locus_tag="ACG89C_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS4900179.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG89C_001638"
     CDS             8895..11876
                     /locus_tag="ACG89C_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS4900180.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG89C_001639"
     CDS             11873..13534
                     /locus_tag="ACG89C_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS4900181.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG89C_001640"
     CDS             13574..14506
                     /locus_tag="ACG89C_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4900182.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZUKR010000061        14342 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000061 DAZUKR010000000
VERSION     DAZUKR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.061"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..81
                     /locus_tag="ACG89C_001641"
     CDS             <1..81
                     /locus_tag="ACG89C_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFS4900183.1"
                     /translation="DTIVLHQRKLDLLKEQKKGFLQKMFV"
     gene            108..443
                     /locus_tag="ACG89C_001642"
     CDS             108..443
                     /locus_tag="ACG89C_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900184.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAKK
                     VYEKKKKGL"
     gene            620..1570
                     /locus_tag="ACG89C_001643"
     CDS             620..1570
                     /locus_tag="ACG89C_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4900185.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2088..2498
                     /locus_tag="ACG89C_001644"
                     /pseudo
     CDS             2088..2498
                     /locus_tag="ACG89C_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2941..3753
                     /locus_tag="ACG89C_001645"
                     /pseudo
     CDS             2941..3753
                     /locus_tag="ACG89C_001645"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3748..3912
                     /locus_tag="ACG89C_001646"
     CDS             3748..3912
                     /locus_tag="ACG89C_001646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900186.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            3852..3974
                     /locus_tag="ACG89C_001647"
                     /pseudo
     CDS             3852..3974
                     /locus_tag="ACG89C_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(4536..5042)
                     /locus_tag="ACG89C_001648"
     CDS             complement(4536..5042)
                     /locus_tag="ACG89C_001648"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4900187.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RYWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRNSKILVPINILKSKISSLVSPLTEEL
                     QKIAMKVM"
     gene            complement(5047..5814)
                     /locus_tag="ACG89C_001649"
     CDS             complement(5047..5814)
                     /locus_tag="ACG89C_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4900188.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6304..6729
                     /gene="lacA"
                     /locus_tag="ACG89C_001650"
     CDS             6304..6729
                     /gene="lacA"
                     /locus_tag="ACG89C_001650"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFS4900189.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6746..7261
                     /gene="lacB"
                     /locus_tag="ACG89C_001651"
     CDS             6746..7261
                     /gene="lacB"
                     /locus_tag="ACG89C_001651"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFS4900190.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7272..8204
                     /gene="lacC"
                     /locus_tag="ACG89C_001652"
     CDS             7272..8204
                     /gene="lacC"
                     /locus_tag="ACG89C_001652"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFS4900191.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8207..9187
                     /gene="lacD"
                     /locus_tag="ACG89C_001653"
     CDS             8207..9187
                     /gene="lacD"
                     /locus_tag="ACG89C_001653"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4900192.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9215..9532
                     /locus_tag="ACG89C_001654"
     CDS             9215..9532
                     /locus_tag="ACG89C_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4900193.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9532..11238
                     /locus_tag="ACG89C_001655"
     CDS             9532..11238
                     /locus_tag="ACG89C_001655"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4900194.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11380..12786
                     /gene="lacG"
                     /locus_tag="ACG89C_001656"
     CDS             11380..12786
                     /gene="lacG"
                     /locus_tag="ACG89C_001656"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4900195.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12809..13081
                     /locus_tag="ACG89C_001657"
     CDS             12809..13081
                     /locus_tag="ACG89C_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFS4900196.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13102..14001
                     /locus_tag="ACG89C_001658"
     CDS             13102..14001
                     /locus_tag="ACG89C_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4900197.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4685 a         2363 c         2765 g         4529 t
ORIGIN      
        1 gacactatcg ttcttcatca acgtaagtta gatttgttga aagagcagaa aaaaggattt
       61 ctacaaaaaa tgtttgtttg atattttaaa tagaaaagag agataaagtg aagtttttaa
      121 attttttgaa ctataaatat ttaaactggg tattatatgg tattacatta ttttttatta
      181 tttattactt ttttaccgat gatgatattt ttttaatttt agcaatactt tttcttgttt
      241 catctaattt tagtgataac ttaattgaag ttcttacaga accagaaatt cggtctatga
      301 cggatgaaga acaacagatt attaaagcta tgatttcgga aaataagaaa gacgtagtag
      361 ttatcaaaaa gattagagag tatacaaatt taggtgtttt acaagccaaa aaagtatatg
      421 aaaaaaaaaa aaagggtcta taattacaaa ttagataata cattagaagc tggagacaag
      481 attgtctaga taatgtgtga gataaaattt ttggcaggtt atcatacata agacaaaatg
      541 tttttaaaac tgataacgta tttattccac aacaattacg catttatttc tgagaaaaat
      601 ataaagcagg aggattacaa tgctgaaaac ttattcctta acaaaagata cattattagt
      661 ttcgtcagaa tcaaatatca aaaattcttc tgttgtttat gtttatggtg acgaacgaga
      721 atatatcgaa tcttctgaac ataaatatga ctttaaaata ggcaatatac taacgtttga
      781 tgatcgagtt gcatatgaca cgatgataga taagaacgat gaaaaaagtt tgctcgtaag
      841 ttttttgttt cccgaggtac atgaaggtgg gcatctagat accctgacga catctgttgt
      901 tttcttggta tttaaaaata agttgataat ttttacaaaa caaaaattaa aattcattcc
      961 cgaactctta tccaatatgg ttttacgtga tgtcaatcat ttcgtggagg aagtactttt
     1021 aaagatcatg caaaaagatt tctatatgat gcttaacgac cttcgtggag tcaaggaaaa
     1081 aattgatgat ttagactcag aaatatcaac gtccggttca ctcaaaccaa cgtttggtga
     1141 tttattgact cttcaaaaat acatgatagc tttgtcaaca acttataaag ctaaccataa
     1201 agcgcttgat ttcattaaaa aaaatttcac aactattgac gatattgatt ttagtactaa
     1261 aaaaataatt gatgatttat acgatacaag tgatacaatg gatagaatca ttttaagtta
     1321 cagccaatat ttggacaaat tggaaaatat gataaacaat atgatttcgt atcagctaaa
     1381 catgattatg aaaaccctaa ccgagatatc aattgttttg actattccag caattatttt
     1441 tggcttttgg ggaatcaatg tccatgtccc gtttgaaaag tcttcttatg gtgttttagc
     1501 cgtattgatt atctctgcta tactaagtct aatttgctgg cttttgatgc gacgcaaaac
     1561 atatttgtaa agtatttgtg ataccctaat atgatttaaa gcagaatttc tttgttgact
     1621 ttttaaaagg ctgattcctt tcaataattt aatagcttca gcggctgctc agaagtcaat
     1681 aaattggact atttttattc agttacttag gcaacatttg agatacgatt gcgattataa
     1741 aaattgatat accaaccaat ggctgcttgg aagactgaca tattagcctc gatatcatcg
     1801 aataactaaa tacagaaaaa tacaataaat attatcaaac tagggactat actagaaaac
     1861 atcagtacat tccttaactc agggtctata attagaaaat cgcccctttg aaaactgatt
     1921 aaaacagccc ccattatcta gtgactagat aatgggggct gtttttttgt taagtgatat
     1981 aataaaccat aaaagaagtt tagaaatacg gtcctggtca tcaataaata aacaatttaa
     2041 ttaattattt atttaattat ttaatttcag aaaggtatac atgaatcatg gtgcaacaaa
     2101 ttgtcctacc catcaaagac tccagcgtct taaagatggt acaagataca cttctcgata
     2161 gtttccgtgc tggtcgccga aattacaccg tttttcaagt tggtaaggcc acgctactac
     2221 gtgtgagtga tgtcatgaca ttaaaaaaat cagatgtcta taatccagac ggctctgtca
     2281 aaaatgcaac ctttattcat gataaaaaga ccggtaaagc aaacacgtta tatttaaagc
     2341 ctgttcagca agacttgttg caatatcatg attggctggc ccaagaaaat atcaattctg
     2401 agtggttatt tccctcgaca gcccattatg atcttcatat cactgagaag cagtttcata
     2461 aggtgatggc gcatgttggt gatctcttag gaatcaactt ggaagacaaa gagaaaaaat
     2521 tgcgagtcag aaaaccgttt tgagatatga acagcatttg ccttcacgcc ttagcgattt
     2581 taaataatag ccttgaaagt gattatggaa aagccctcta cgctcaatgc aaaattgacg
     2641 tagaaactat ttttgatcga ttgaaaggtg tatttggtat gtgaagaacc catgtcagag
     2701 gcaagaacgt tgcacctact gacattggaa tgatgcttat gagcatgaat ctaaccaaat
     2761 tatcccttga ggctagaaga aaagcggaag cttttcaaca caaagcagcc aaaaataaaa
     2821 aacgcgacga agcaatcaca tttatgatta ttcatcgcgt tttttatatt tagagctagt
     2881 tatttgctag acccactatt tacagatgaa acgtttatca cctttggttt tgggcattta
     2941 gtggaattag cagaaccagg tcattatgac gaaaagtggc aaaattggaa acttgaatct
     3001 ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg aaagcagttt
     3061 aaaattgttg cagaacttct caaaaaggca aatacaatta ttgttgcaac agatagcgac
     3121 agagaaggtg aaaatatcgc ctggtcgatt atccataatg caaatgcctt ttcaaaagat
     3181 aaaacattta aaagactatg gatcaatagc ttagaaaaag atgtgatccg aagcggtttt
     3241 caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa
     3301 attgccgatt ggttgatcgg catgaacgca agccctttgt atacgttaaa tttacaacag
     3361 aagggcgtac aaggtacatt ttcactagga cgtgttcaaa cgcccacctt ataccttatt
     3421 tttcagcgcc aggaagccat agagaatttt aaaaaagaac cttttttcga ggtggaagct
     3481 aagtaaaaca agggtcattt aagggcgttc taagccccac acagcgtttt aaaacccaag
     3541 aggagctttt tagcttttgt ttcttctaaa caagctaaaa taggcaatca agaggggata
     3601 attgctgatg ttcaaaccaa agagaagaaa acgaatagtc cgagttcgtt ttctttaagt
     3661 agtttgcaat caaaagtcaa tcagctttat aaagcgacag cgagccaaac tttaaaagct
     3721 atgcaaggac tgtatgaagc aaaattattg agttatccaa gaacagatac accatttatt
     3781 acagagaacg aatttgctta tttaaaagcg aattttggca aatatagcgg ttttttagga
     3841 cttgatcttg aaatgttgtt atcattgata atgatacgtt caaacaacag cacccaaatt
     3901 ttgacgaatt agtgaaactc tatgaaaaag acgtagtaaa acatgcaaca tcctattcta
     3961 atcagctggt aaagcttaat taggttatat aagcttgaaa ataaagggtt tgtccaatat
     4021 ggacaagcct ttttcttatg ttttgccaca gatttgccac acttattgtg taagagtatc
     4081 aaggataggt ataatttgat actttgtttt tttagtgatg tgcaaataaa tttctgttac
     4141 acgactacct ctagaatgcc ctacacggtc ttgaattgct tctaggggta ttccttactc
     4201 tgctaagaaa ctaatatgcg tatgacggaa tatatgggtc gtaatatgtt tattaaaact
     4261 aagtttatta tcaatatcaa ttcctgctcg ttttggatta ccttttcgat ttaattttcc
     4321 attttcaggt gagttgagga aattgttaag gacactataa tgaagaggtg ttccattata
     4381 gtcgggttta tgacgacttt tggaagtaaa aatccatcca ttatctttat aatcaggatt
     4441 gaatctcata tgttggtgat ttaactcaat ttcctcttta agaatttcta tagccctttt
     4501 agataattgg acatttcggt aactttgtaa gttttttaca ttaccttcat agctattttc
     4561 tgcagctcct ccgtgagtgg actgactaat gatgatattt tgcttttcaa aatattgata
     4621 ggcaccaata ttttggagtt tcgactctgt aagtcaacag ggccattagt tagaatacct
     4681 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
     4741 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
     4801 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
     4861 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaatatct gaaacgtttg
     4921 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
     4981 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
     5041 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
     5101 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
     5161 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
     5221 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
     5281 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     5341 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     5401 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     5461 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc caggtccaat
     5521 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     5581 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     5641 caattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     5701 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     5761 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     5821 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     5881 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     5941 ataagaacag aggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     6001 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     6061 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     6121 gttatctatt ttttaaaaaa acaaataaaa aaaacaaaaa ataaacaaaa aaagtgttgc
     6181 tttttgtttg aatgcttgat attatataaa caaagaaatg atgaaaacgt tttttcgaac
     6241 attttgcaaa atgttttcta cttctacgta gcatttcttt ctaaaattta ggaggtggtc
     6301 aaaatggcta ttgttgttgg tgcagatctc aaaggtacga gattaaaaga tgttgtgaaa
     6361 aattttcttg tagaagaagg ttttgaggta attgatgtga ctaaggacgg acaagatttt
     6421 gttgatgtta ccttggcagt tgcctctgaa gtaaataaag atgagcaaaa cttaggtatt
     6481 gtaattgatg cttatggagc tggtccattc atggtagcaa ctaaaattaa aggtatggtt
     6541 gcagcagaag tttcagatga acgctcagct tatatgacac gtggacataa caattcacga
     6601 atgattacgg ttggcgctga aatcgtcggt gatgagcttg ctaaaaacat cgctaaaggt
     6661 ttcgtcaatg gtaaatatga tggcggacgt catcaaatcc gagtagatat gcttaacaaa
     6721 atgtgttaag aaaaggagtt aaggaatgag aatcgcaatt ggatgtgacc acattgtcac
     6781 agatgaaaaa atggcagtat cagaattctt gaaatcaaaa ggacatgagg tccttgattt
     6841 cgggacatat gaccacgcac gtactcacta ccctatctat ggtaaaaaag tcggtgaagc
     6901 agtagtaagt ggtcaagcag acttgggagt atgtatctgt ggtacaggtg tcggcattaa
     6961 caatgcagtc aataaagttc caggtgttcg ctcagcgttg gttcgtgata tgacatcagc
     7021 tatatatgca aaagaagagt taaatgcgaa tgttatcggt ttcggtggaa agattactgg
     7081 tggtcttctt atgaatgaca tcattgaagc tttcattgaa gctgagtaca aaccaacaga
     7141 agaaaataaa aaattgattg caaaaatcga acacgttgaa acacataatg cacatcaagc
     7201 agatgaagat ttctttacag aattccttga aagatgggac cgtggtgact accacgatta
     7261 aggtataaca aatgattctg acagtcacac taaatccttc agtagacatc tcttatcctc
     7321 tagaaacatt gaaaattgat acagtgaata gggtgaaaga tgttagtaag acagctggtg
     7381 gaaaaggctt aaatgttact agagtcttgt atgaatctgg tgataaagtt actgctacag
     7441 gtttcttagg tggtaaaatt ggtgaattta ttgaaagcga attagaacaa tctcctgtta
     7501 gtccagcttt ttataaaatc tcaggaaaca ctagaaactg tattgctatc ttacacgagg
     7561 gtaaccaaac agaaattttg gaacaaggac caacaatttc tcatgaagaa gcagaaggct
     7621 ttcttgacca ctatagcaat cttattaaac aatcagaagt tgtgacaatt tcaggaagtt
     7681 taccttcagg acttcccaac gattattatg aaaaacttat ccaacttgct tcggatgaag
     7741 gagtagcagt cgttttggat tgttcaggtg cacctcttga aacagttcta aaatcgtcgg
     7801 ctaaacctac tgccatcaaa cctaataatg aggaactttc acagttgctt ggaaaagaag
     7861 taacaaaaga tattgaagaa ctcaaagacg ttcttaaaga gtcacttttt agtggtatcg
     7921 aatggatagt ggtctcatta ggtcgaaacg gtgcctttgc aaaacatggt gatgttttct
     7981 ataaggtaga tattcctgat atcccagttg ttaatccagt cggatcaggg gactcaacgg
     8041 tagctggtat tgcatcagct ttaaatagta aaaaaagtga cgctgactta ttaaaacatg
     8101 cgatgacatt gggtatgtta aatgctcaag aaacaatgac agggcatgtg aatatgacta
     8161 attacgaaac actaaactca caaattggag taaaagaggt ataaaaatgg tacttacaga
     8221 acagaaacgc aaatctttgg aaaaaatttc agataaaaac ggtgttatct cagctttggc
     8281 atttgaccaa cgtggtgctt tgaaacgttt gatggcacag tatcaagata cagaaccaac
     8341 tgtagctcaa atggaagaac taaaggtttt ggttgctgat gaacttacaa aatacgcttc
     8401 ttcaatgttg cttgaccctg aatatggact tccagcaaca aaagcattgg ataaagaagc
     8461 aggtcttctt cttgcctatg aaaaaacagg ttatgataca tcaagtacaa aacgtttgcc
     8521 tgactgcttg gatgtttggt ctgcaaaacg tatcaaagag caaggcgctg atgccgttaa
     8581 gttcttgctt tactatgatg tagacagctc agatgaactt aaccaacaaa aacaagctta
     8641 tattgaacgt gttggttctg aatgtgtcgc tgaagatatt cctttcttct tggaaatcct
     8701 tgcttatgat gaagaaatct cagatgcagg ttctgttgaa tatgcaaaag tgaaacctcg
     8761 caaagtcatt gaagcgatga aagttttctc tgatccacgc tttaacattg atgttcttaa
     8821 agtagaagta ccagttaacg ttaaatatgt cgaaggtttt gctgatggtg aagtagttta
     8881 cagtaaagct gaagctgctg acttctttaa agcacaagaa gaagcgacta accttccata
     8941 catctaccta agtgcaggtg tatctgctaa attgttccaa gaaacattgc aatttgctca
     9001 cgactcaggt gccaagttta atggtgtgct ttgtggacgt gctacttggg caggatctgt
     9061 agaaccttac atcaaagaag gtgaaaaagc agcacgtgaa tggttgcgta ctactggatt
     9121 cgaaaatatt gatgaactta acaaagttct tgttaaaaca gctagtccat ggactgataa
     9181 agtatagtaa aaacataaaa cggaggatat tgttatgaac agagaagaga tgactctctt
     9241 agggtttgaa attgttgctt atgctggaga tgctcgctct aaacttttag aagcgcttaa
     9301 agcggctgaa aatggtgatt tcgctaaggc agatagtctt gtagtagaag caggaagctg
     9361 tattgcagag gctcacaatt ctcagacagc tatgttggct cgagaagctt ctggggagga
     9421 acttccatac agcgttacca tgatgcatgg tcaggaccac ttaatgacta ctgtcttatt
     9481 aaaagatgtg attcatcacc tcatcgaact ttataaaaga ggagcaaaat aatgcataaa
     9541 ctcattgaac ttattgagaa agggaaaccg ttctttgaga aaatctctcg aaacatctat
     9601 cttcgtgcta ttcgtgatgg atttatcgct ggtatgccag tcatcctttt ctcatctatc
     9661 tttatcctta ttgcctatgt cccaaatgct tggggattcc actggtcaaa agacattgag
     9721 accttcttga tgactcctta tagctattcg atgggtatcc tagcattctt tgttggtggt
     9781 actaccgcta aagctttgac ggactcaatg aaccgtgacc tacctgcgac caatcagatt
     9841 aacttcttat ctacgatgct agcgtctatg gtagggttcc ttttgatggc agctgagcct
     9901 gcaaaagaag gaggcttctt gacagccttc acgggaacaa aaggtctttt gacagcattt
     9961 attgcagcct ttgtgacagt taatgtttac aaggtctgtg tgaaaaataa tgttactatt
    10021 cgtatgcctg aagaggttcc accaaatatc tctcaagtat ttaaagactt gattccattc
    10081 actgtagcgg ttgttctcct ttatggattt gaactcattg ttaagggaac tcttggtgta
    10141 accgttgcag aatcaatcgg tacccttctt gctcctctct tctcagctgc agatggttac
    10201 cttggaatta cacttatctt tggtgcttat gccttcttct ggtttgttgg tatccacggt
    10261 ccttcaattg tcgaaccagc aatcgctgct atcacttatg ccaatatcga tgccaacttg
    10321 catcttatcc aagctggaca acatgcagac aaagttatca cttctggtac tcaaatgttt
    10381 attgttacca tgggtggaac aggagctaca ttgattgttc cattcttgtt catgtggatt
    10441 tgtaaatcag aacgtaaccg tgccatcgga cgtgcctcag ttgttccaac attctttggg
    10501 gttaatgagc caatcttatt tggtgcacca atcgtattga atccgatttt ctttgtaccg
    10561 ttcattttcg ctccgattgt caacgtttgg atctttaaat tctttgttga cacattgaac
    10621 atgaactcat tctctgccaa ccttccatgg gttactcctg gtccgttggg aattgtactc
    10681 ggtactaact tccaagttct atcatttatc ttagccggac tcttggtagt tgttgatact
    10741 atcatttatt acccatttgt taaggtatac gatgaacaaa ttcttgaaga agaacgttct
    10801 ggtaaaacaa acgatgctct taaagaaaaa gtagctgcaa acttcaatac tgctaaagca
    10861 gatgccgttc ttggaaaagc aggcgttgct aaagaagatg tagcagcaaa caataatatc
    10921 acaaaagaaa ctaatgtcct tgttctttgt gcaggtggag gtacaagtgg cttgcttgcc
    10981 aatgccttga acaaagcagc agcagaatac aatgttccag tcaaagcggc agcgggtagt
    11041 tacggtgctc accgtgaaat gttgccagag tttgacttgg ttatcttggc tccacaggtc
    11101 gcttcaaact tcgatgatat gaaggctgaa acggataaat tgggcattaa acttgcaaaa
    11161 actgaaggag ctcaatatat caaattgaca cgtgatggac aaggtgctct tgcatttgtt
    11221 caacaacagt ttgattaata aatttgtaaa agttatttga gacgagatta ttctttcaat
    11281 tccttataat attgttaacc atatcattgc atttcatttc cttggtttcc ggaactcaga
    11341 atatcgtctc aaatgctttt tttgaaagga tttatagtta tgactaaaac acttcctaaa
    11401 gattttattt ttggtggtgc tacagctgct tatcaagcag aaggtgcgac tcacacggat
    11461 ggtaaaggcc cagttgcatg ggataagtat cttgaagaca attattggta cacagcagag
    11521 cctgcaagtg atttttacca taaatatcct gttgatttag aattagctga aaaatatggc
    11581 gttaatggta ttcgtatttc aattgcctgg tctcgtattt tcccaactgg ctatggcgaa
    11641 gtaaatgaaa aaggtgttga attctaccat aatctttttg ccgagtgcca caaacgtcat
    11701 gttgagccat tcgtgacttt gcaccacttt gacacaccag aagttcttca ctcagatgga
    11761 gatttcttaa accgcgaaaa tatagaacac tttgtagatt atgccgcttt ctgttttgaa
    11821 gagttcccag aagtaaacta ctggacaact ttcaatgaaa ttggcccaat tggagatggt
    11881 caatacttgg ttggtaaatt ccctccaggc attcaatatg atcttgccaa agtcttccaa
    11941 tcacaccaca atatgatggt ttctcatgca cgtgctgtga aattgtataa agataaaggt
    12001 tataaaggcg aaattggtgt agtacatgcg ttgccaacca aataccctta tgatccagaa
    12061 aatccagcag atgttcgtgc cgctgagtta gaagatatca tccataacaa atttatcttg
    12121 gatgcaacct accttggaca ctattcagat aaaacgatgg aaggggtcaa ccacatccta
    12181 gctgaaaatg gtggagaact tgatcttcgt gatgaagact tccaagcact tgaagcagct
    12241 aaagatttga acgatttcct tggtatcaac tactacatga gtgattggat gcaagccttt
    12301 gatggtgaga ctgaaatcat tcacaatggt aagggtgaaa aaggaagttc taagtaccag
    12361 attaaaggtg ttggtcgccg aatcgcacca gattatgtgc ctagaacgga ttgggactgg
    12421 attatttatc ctgaaggctt gtatgaccaa atcatgcgag tgaaaaatga ttatccaaac
    12481 tacaagaaga tttacatcac tgaaaacggt cttggataca aagatgagtt tgtagataat
    12541 accgtttatg atgatggtcg tattgattac gtgaagaaac acttggaagt tttatcagac
    12601 gcaattgcgg atggtgcaaa tgttaaaggt tacttcatct ggtcacttat ggatgtcttc
    12661 tcatggtcaa acggatacga aaaacgttat ggactattct atgtagactt tgacacgcaa
    12721 gaacgctatc ctaagaaatc agctcattgg tataagaaag tagcagaaac tcaagtgata
    12781 gagtaatatt acttgaaaat gtaaaaaatt gttatccgat actttagaaa aagtacaaca
    12841 cttatttaga ccaggctttg aaaaggtata tattgttagt ttcgaagatt gtcctatgat
    12901 tcctgagctt gaagctaccc cgttgctaaa atgtggtaaa tggtatgttt caactggaaa
    12961 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
    13021 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
    13081 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
    13141 tttaaaacac ttggtggtca actgacttcg attaaagaca aggatgggat agagtatctc
    13201 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
    13261 agtttacgaa atgattgggc tatttataga cctcaagaaa gacccttttt tacaggtctt
    13321 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
    13381 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
    13441 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
    13501 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
    13561 cctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
    13621 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
    13681 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
    13741 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
    13801 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
    13861 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
    13921 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
    13981 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
    14041 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
    14101 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
    14161 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
    14221 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
    14281 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    14341 gt
//
LOCUS       DAZUKR010000062        14157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000062 DAZUKR010000000
VERSION     DAZUKR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.062"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..1823
                     /locus_tag="ACG89C_001659"
     CDS             186..1823
                     /locus_tag="ACG89C_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900198.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1968..2708)
                     /gene="truA"
                     /locus_tag="ACG89C_001660"
     CDS             complement(1968..2708)
                     /gene="truA"
                     /locus_tag="ACG89C_001660"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4900199.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2899..3534)
                     /locus_tag="ACG89C_001661"
     CDS             complement(2899..3534)
                     /locus_tag="ACG89C_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4900200.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3681..4901)
                     /gene="sstT"
                     /locus_tag="ACG89C_001662"
     CDS             complement(3681..4901)
                     /gene="sstT"
                     /locus_tag="ACG89C_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4900201.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5120..6118
                     /locus_tag="ACG89C_001663"
     CDS             5120..6118
                     /locus_tag="ACG89C_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900202.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6314..8149
                     /locus_tag="ACG89C_001664"
     CDS             6314..8149
                     /locus_tag="ACG89C_001664"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4900203.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8209..8925)
                     /locus_tag="ACG89C_001665"
     CDS             complement(8209..8925)
                     /locus_tag="ACG89C_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4900204.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9152..9694)
                     /locus_tag="ACG89C_001666"
     CDS             complement(9152..9694)
                     /locus_tag="ACG89C_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4900205.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9699..9926)
                     /locus_tag="ACG89C_001667"
     CDS             complement(9699..9926)
                     /locus_tag="ACG89C_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4900206.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10005..11885)
                     /locus_tag="ACG89C_001668"
     CDS             complement(10005..11885)
                     /locus_tag="ACG89C_001668"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4900207.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12074..12688)
                     /locus_tag="ACG89C_001669"
     CDS             complement(12074..12688)
                     /locus_tag="ACG89C_001669"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900208.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12830..13390
                     /locus_tag="ACG89C_001670"
     CDS             12830..13390
                     /locus_tag="ACG89C_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4900209.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13695..14009)
                     /locus_tag="ACG89C_001671"
                     /pseudo
     CDS             complement(13695..14009)
                     /locus_tag="ACG89C_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         4419 a         2738 c         2216 g         4784 t
ORIGIN      
        1 taagaactac taaaagaaat gcttttttac ttattttcat agatttgttg ttctcctttt
       61 ttaattctat tttcagtata ctagaattgt ctttttttaa taagttttgc gttaaatttt
      121 tcatagcact agtatgatat actagaaaag aatcaatttt tattactttt aaataggaga
      181 aaaaaatgaa aaaaaacaaa aaccaaataa tagatatcat ttttatgtta tttctaagtt
      241 ttgcttacgt catccctcta ttcatgtcta agggaatcta tcatagtgtc aatcaggata
      301 cttactttca tttatctcga atcataggac tagataatgt atggagttct ccagtaaact
      361 tcaataactt cgatcatcat ggaacaatga tgaatatttt ttatccatgg cttactcttt
      421 atccagcttt tttattttat aaaatgatgg gaaatcttgt tttaagttat aatatctact
      481 atttttttat cactttttta acaatggtcg tttcttattt ttctatgaaa caaataaaaa
      541 acaatcgtta tatttcgctg ttattttcga ttatttatac attttccgcc tatcgtgcca
      601 ttgacatatt ccatcgtgct tcattgggag aagctgtcgc tttaacattt ttgcctttga
      661 tactaatggg gtgttacgaa atttatatta gagattacca aaaatggtac tggttgtcta
      721 ttggcatgac gctagtagtg tatactcatt tactttctgt cgctatggtt tctgtattta
      781 ttggaggaac gttgtttctt tctttttatt tttgggacca aaaaatcgct agactcttaa
      841 gtcttttaaa agctaccgtc ctcacctttt ttttaagtgc gggattctta attcctttta
      901 ttcaacaatc tagagcacaa gaattgaaag tcccactggg aaaagaatta agtggtatgg
      961 ctcctagtga tatgctgaca catattttaa ataataacta taacaactat actattggat
     1021 tattcttatt tctcggattg attggtgctt ttatttttat aaaaaaatta actgctgatg
     1081 atttatttat tttctttctt ggtgtatttg ttcttttttg ttcaaccaat ttatttcctt
     1141 ggcaactttt taaccataca ccagttaaga gtctacaatt tgtctggcgg ttaaatggtt
     1201 tttcttcttt atttatagcc tatacgatga gtatagtgat ctattatatt ttttctacta
     1261 aaaataattc atggaaaata atggtcttta cgtttcttgc actaatattg catctctcag
     1321 gagtaagcaa ttctatccat gctaacaaag attcattaac acttatagca cctgaagacg
     1381 ctgttgctat agctgaaaat tacaatcata ctgattatgc aaacaaagag tcaatctatc
     1441 atcctgatat gattaataat gatcaatatt tactaggtaa ccaggaaata aatccaacaa
     1501 ctcattttat gagtaacaaa cttacaattg aattagataa ttcgaataat aaaaatactg
     1561 tcttgacgac acctatttat cgttataaag ggcaagtggc ttctattaat ggcaaactag
     1621 tacaaacaaa attatctaag tttggcacaa ctgaattaac tattcctcca ggtataaata
     1681 aagtcgttat tacttatcag tacacaaaac tagcaattgc ttcaagatat ttatctatcg
     1741 ttactttgat tttattttta ctttatcgat ttactttttc taaacaaaaa caaccgagaa
     1801 gagaagtaca acattctcac tagattttta tttaagaaag actttttagc tgtacacata
     1861 aacaggctaa aaagtctttt tttatatttc tttaatttgc ttcatacatt atttaacatc
     1921 gatatttata ctgtgaaaca tgcgtttcat ccattatcaa agtattttta tcgataataa
     1981 acttcatcca aaaacagacc ttgtgcaggg acggtttctc ctgcatgttg ccgcactttg
     2041 ctttcaaata tttcatcgat tgtttctatc ggcatggcac ctgtgccgat ttctagtaat
     2101 gtccccatga taatccgcac cattttatat aagaatccgt ctccgacaaa agtaaaatga
     2161 agcatttctc cctttcgttc gatagataat tcatcaatcg tacgcactgt ggattttttt
     2221 gattttttca atgcagaaaa accaatgaaa tcatgtgtac ccactaattt ttcgcaagct
     2281 gcattcagct tgtccatatc caactcttga gggaaataaa agctgtggtt tcgctcaaat
     2341 gctgaaggaa tcacgttgtt ccaaacataa tagctgtatt ttttgcccac cgcattatat
     2401 cttgaatgga agcgctcagg catttcttcg attcttttga tgacaatatc ccgcggaagg
     2461 taacgattca taaagtcgat catttctgcc agtccaattt ctgtgtttgt tttaaaattt
     2521 gctacttgtc ctcgcgcatg ggtgccagca tctgttcttc ctgatccaat aatttctatc
     2581 tcttcatttg tcatctgatg caatactttt tcgatttttc cttgaatggt tttatcgtca
     2641 tctccaagtc tttgccatcc tgaatatctt tttccgtcat attcgattgt caacttgatg
     2701 tttctcatat tttagtggct cctctgtctt ccatttggtc ttctctctac ctttttaaga
     2761 ttgtagcaca aaatatagct ccttgcttct ctaagacaga agctatacaa gaaaactgtg
     2821 gaaatatata tttcagaaaa acaaaaaaca gccaccggat gaagtatccg ctgactgttc
     2881 aatttgacgg ctcaagtttt actgtttgag atgcaagata ctcaacctgg caatgggcac
     2941 ataattaaag aaaaaactga ttctttggca aaaacctatt ttattcagta atggccaaga
     3001 atttatagaa ggaattcttg ccagtccata aatcgccgca aaaagcaaac tgatcacaga
     3061 cagtccaagg tataagtggc ttacgtccat ttgcccattt tttcgataga tcaagaaaag
     3121 aagaactgga aacaataaaa aaccactgta acctaaacct gatccgatat tatggaccca
     3181 tgtcgagaca ttccacgaag attcatgggt attgatactg aacaaacctg taaagataca
     3241 atcacctata ccatagaacg cgatggctgc tgtaactagg gtagctaaca cacgagatgt
     3301 actaacaaat gcagcataca atgcaggcaa tgacaggatg aaaaagatcc ctgaaaccac
     3361 agaccaaata agaaacgctt ttcttacagg actggaaacc tctccaaata cactgatcac
     3421 gctcttcatt tgatctaatt caggataata caatcctaat acgtacggag tcaaaaaatc
     3481 actcagcacg cctaataata aaaagaaaaa accaaattta cgtaaaaaat ccatgatacg
     3541 cctccaataa caattaccta tgcttttaat tgttattata aacgacttta tcttgattta
     3601 acagaaacct ctatttacac gaacatctat ataactcatt aaaaactcga atcattttaa
     3661 aaatactaaa tatctaacgt tcaacgttca cttaatccat ctaaacaatc tttcaatttg
     3721 attttttccc cacttctttt acgcgcgcct agctctccta ttgcagtaaa caacagatca
     3781 cttgacgaat ttaaggcagt ttccattgaa tcctggataa caccgataat aaatccaata
     3841 ccgacgattt gcatcgcgac atcattagag ataccaaaca aactacatgc taatggaatc
     3901 aataacagtg aaccgcccgc aacacctgaa gctccacaag cagctaaagc agacaaaata
     3961 cataaaagga ctttcaaaac aaaaggtgct tcaatcccta aagaatgtac agcagtcaac
     4021 gtcatcaagg aaatggtaat cgcggcaccg cccatattga tcgtagcacc taacgggatc
     4081 gatatggaat aagactcttt tgtcaatttc aatctttcag ccaacatcat gtttatcggg
     4141 atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg cgctttcttt caaacaaaag
     4201 aaaatcaatg gatagggatt ttctttaata aacaaaaacg tgaggaatgg gtacacaact
     4261 aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa gctgcagata ctctgccaat
     4321 ccagcgattc cagtcgtagc aattgattga taaaccaatc ctaaaatccc tatcggagca
     4381 aatgcaataa tcacttgtac gacttgtgac aacgctatag atatctgatc caccaactct
     4441 tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca atacagccca gaataagacg
     4501 ccaaggtaat tcgcatcaat aagagattga acaggatttt gtgtgacatt cgtcaacata
     4561 gtagataaaa cactttccaa actttgcggt gctttttcag atactgtttc ttgtaaagta
     4621 ataggaacag taaaaattga acttacaata actgctacaa gtgcagaaag caatgttccc
     4681 actaaatata agatcaaaat aggcttcacg aatgtcttat tcccaatttt atgcttggca
     4741 attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga ttgccttcaa gcaactgaca
     4801 aacaacgttc ccaatattga aataaatgtc catgtgggga aaaagatacc aaacaatatt
     4861 ccgactaaaa taccaacaac aatacgttga actaacgaca tatccaaaaa cttctctaac
     4921 aaatgaccac cctttcacaa ttttaacaat cattgtatca ttttatgcaa agaaactaaa
     4981 ggtataattt tctaaaaatt ctaacaatac aaacaaccat ttcttgctaa ttttgtactt
     5041 tttctgtcta cacgttgttt tttgagatat tctaagttag gatgttatta atgggcaact
     5101 tatcaggaaa cgttttctta tgtttttgtc caattttttt gtgaggtgtt tattcttgaa
     5161 aaaaataaag aaggagagtt atggcttcct gacaatcttt gtcgcttgta taatgatggt
     5221 tttacagcgt atattgattt cagcagtcac tattcctaca tatgcaacaa accctggtcg
     5281 attctacatc tatacatttt tacaaacttc cgttgttatt ggtgcaaatt tgatcccact
     5341 ttacatgggt taccaatatc aaaaaatcgc ccgtttaaaa gttttacatt atttaagtcg
     5401 ttttgccttt atattcctaa tagccacact agtttgcaat atcttttttt acgtacaagc
     5461 tggcagcttg aatattcgtg attattggct tatcctaaca cctatcagtc agaattactt
     5521 tacttatgct gtttcttgta ctttgctttt ttgtagtatt ccttatacag ttggattttt
     5581 aaataaatta tcgaaaacaa caattaaata tctactatta tttttaacaa ttggattagt
     5641 tggctgctct actctattta ataaagaccc gtttgcatta cagaatggac agagtatttt
     5701 atggattttt tatttatttt taatgggtta tggtttgaaa gaatggaact ggaaagaaaa
     5761 ggtccgctat aagcttcctc aagtatttat cagcttgatt gttttattcg gtttgattat
     5821 tttgatgaca caaatttcac tcatcatcag aggaaataca gatacagcgc tgaggttttc
     5881 taaaccatat tctctcttcg caatgtatta tactttctct tcgtttttat ttttagaaat
     5941 gctatctgat aaattaggta taaaaggaag aggaaatttt cttgctgtat ttttgattgt
     6001 cactcaagtt atatgtaatt tccctttagt ttcttatcgc gtatctacat ttatgaaacg
     6061 tgaatttcca aactcaggga aggcatggct gataaatatc gggcagtttt ttatttgata
     6121 tttattagca gtgtccctcc tagttatctt tttattttat atacaaaaaa tccgtttcat
     6181 tcatcgtttc atcgaatact tcgcttttga ttcgattgaa caattaactc agcgttggaa
     6241 aaaaagaatt cattggcttt ttgttcataa aagttatttt ttagttgcag cattttttta
     6301 ctgctttacc ttcgtgcaga ttttcgtttt agaaccaaaa gaaggttgga aagaaactat
     6361 ccaggttgcc ttaaaaatat ttactcagcg acaagcaccc atgatcctga caatcatcat
     6421 tattatggga tttttcttct tactattgtt gatttccaat cgtttttggt acgccttgac
     6481 tgctaccttg atcattaact tactgctcac tatttcgaca gtaatcaaaa tggaaatgcg
     6541 ggaagagcct gtctttcctt cagatttaaa aatgctgacc ggattgtcag aactattatc
     6601 gatggttagc ccagtcttat taatcgtggg cggattgatc cttctcctat tattgattac
     6661 tagtattatt gtacaacgta gactacaaaa acagtattcc ttgaagattc attggaaacg
     6721 tcgatttatc agtatcgctg tattattagg atgtttttcg ggtgtctttt tcattaatca
     6781 caaaaattcg ccttctttct tgctgtttaa tttatttaaa gtaaacaaga ctttctttaa
     6841 ccagcaaggc gccgtaaaag ataacggacc agtcattcaa ttcctaaaca atatcgacat
     6901 taaaatcatg gaagaacctg ctggctattc gaaagaaaaa attaatggaa taatgaaaaa
     6961 gtacaacaca aaggcagatg aaatcaacca aacaagaagt gactggttaa acaatcagac
     7021 gatcgttctc aatttaagtg aaagcttcag tgatcctgca cgtgtcccta atctgacagt
     7081 acctacagac cctattcctt acatcaaaag cattataaca gaaggaacag gaggattgat
     7141 gttatccgtc ggttatggtg gcggaacagc taacatggaa tgggaaggac tgacaggctt
     7201 gagtatcagt aacctgtctg cttcgttagt tacaccttat acccagctag tggagcgtca
     7261 gaaaataagt ccaaatatta ccgatctatt cgatgaaaaa atagccatcc atccttttac
     7321 agctgctctt tatcgcagaa aacaagtgtt tgataaattt ggttttgaca ggttctatta
     7381 tttagacagt ccggataaat taacttatac ggataaaata gaacaaagcc cacgcatatc
     7441 ggatgattca gcatacaaag aaaccttgaa agctttacaa agcaatacgg aaacgtcaca
     7501 attcatccaa ttatccacta tgcaaaacca tatgccttat gatgattact atagtgaatt
     7561 gaactacact gctgaaggaa ctgctgtcat tcctaataga agaaaagaat tacaaactta
     7621 tatgcaaggc cttcactata ccgatacagc agtaaaaaaa ttcatccaag aaatcgacaa
     7681 aatcgataaa ccgattacct ttgtttttta tggtgaccat ttacccgcaa tctatagtgg
     7741 gaatgattcg aaaaaatatg gattagaaca gcaccagaca gattatttca tttataacaa
     7801 caagtatagt cgtgaacaag cagctacgtt aaataaagaa gttgttgctc ctaataactt
     7861 tggtgctatg gctcttgagc atgcgaatat caaagtaact ccttactatg cgttgttgac
     7921 agaagtatct aatcatctac ctgcattaac aattgatcct acagaaagct tgtctaatcg
     7981 ttttaatgga aaacaggtct ttgttacaga ggatagtcaa attatccaag aaaaaaattt
     8041 aactgagaaa caaaaagaaa ttctagaaga ttatcgtctg atacaatatg atcttgttgc
     8101 aggtaaccag tacagtgcaa catgggcgac acaaaaaata aaagactaac aaataaactg
     8161 agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt aatttgcttg
     8221 tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct cgttgaagta
     8281 aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg gctgattttg
     8341 ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg catcggaata
     8401 gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt ctgcagaaac
     8461 aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag ggactttaag
     8521 accatataag tccacactca tttgattcgt ataacttttg tattgttgtc ccattgtttg
     8581 gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc acagtgtttg
     8641 ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag aacttgaagt
     8701 agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg tatctgctgt
     8761 ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag ttgcaactgt
     8821 tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg tacaaccagc
     8881 taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa aaactccttt
     8941 cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg atttttcgta
     9001 ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga tgttgtctgt
     9061 aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt tacctcacgc
     9121 tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc tcatccaaat
     9181 cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct ttattgaaac
     9241 ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca gctaatttga
     9301 ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt tccaacattg
     9361 ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa cgcatgactt
     9421 cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag attttattga
     9481 accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct tgcgaagatg
     9541 tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta aaatttgcta
     9601 atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct gcttgttttg
     9661 tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt tacttttgct
     9721 gattggactt cataacctaa tttttccaca actgcagcta attgttcagg gtggacttca
     9781 tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt attgcttacc
     9841 ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca agctagtgta
     9901 tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt gatagtctta
     9961 ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga tttttcttct
    10021 ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat caagatacta
    10081 gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa gccgttgagt
    10141 aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa aacagttttc
    10201 ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa gacaacgtca
    10261 ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc agccgcagcc
    10321 aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc ttgttcttgg
    10381 tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac ttcatcaatg
    10441 cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag catcactaac
    10501 tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg tcgaatcaca
    10561 tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat gaccgagctg
    10621 cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga aagtttgtga
    10681 gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc agctgtgatt
    10741 ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc aatgtctttc
    10801 tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt ttctaatcga
    10861 gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt ggatacagca
    10921 gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat tttggaaaaa
    10981 gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt accgattcct
    11041 gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag aaccaaaaca
    11101 gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat aagtaaaacg
    11161 gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga tttggtatct
    11221 tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc gaccttagtt
    11281 gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc atctccactg
    11341 attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata ccctttcccg
    11401 tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac gatatcgcct
    11461 tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac aacactagca
    11521 gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt caatgttttt
    11581 tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc cttgtattct
    11641 ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga gataactttg
    11701 actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat agaagctatg
    11761 gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa accaaaaata
    11821 atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca cttttgtaat
    11881 ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat acttatccat
    11941 cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt ggtagaatac
    12001 actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac tattgtcgtt
    12061 tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga tctatttctt
    12121 tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca cgtcgttcat
    12181 agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct ttctctcttt
    12241 tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca ggatttgcag
    12301 ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca agcatttgtt
    12361 catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt aaaaaatcga
    12421 atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct ccttttatct
    12481 ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt tgagttgctc
    12541 gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt tcattttcca
    12601 tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg taatctttct
    12661 tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa tgattgtgtt
    12721 atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct ataagtttgt
    12781 cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa tgaaaaagac
    12841 agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca cattattagt
    12901 aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag atgtacatcg
    12961 ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat tagaaagcaa
    13021 cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct atgaggaact
    13081 atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct tatttatcct
    13141 caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag aaaactatct
    13201 gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga gattctttac
    13261 tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa gcaattacaa
    13321 gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca tagagaaaat
    13381 catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct tctcgtataa
    13441 caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa atttctataa
    13501 ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt gatatatctt
    13561 gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa tttattacaa
    13621 cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca tccagtttat
    13681 tgaattcttc tcgtttacct caattcattt tcaaggggaa tcagctcatc atctaacatt
    13741 ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc tggatgtttg
    13801 gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg aattggatct
    13861 tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac aaaccactca
    13921 ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg tttaatgaca
    13981 aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat tcaaagataa
    14041 ttttctcaat aaagattttt gattccagta agattccttt actcctttta caacatagcg
    14101 ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag cccaatt
//
LOCUS       DAZUKR010000063        13810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000063 DAZUKR010000000
VERSION     DAZUKR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.063"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACG89C_001672"
     CDS             complement(221..1423)
                     /locus_tag="ACG89C_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4900210.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACG89C_001673"
     CDS             complement(1426..1620)
                     /locus_tag="ACG89C_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4900211.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACG89C_001674"
     CDS             complement(1667..2515)
                     /locus_tag="ACG89C_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS4900212.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACG89C_001675"
     CDS             complement(2720..3070)
                     /locus_tag="ACG89C_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900213.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACG89C_001676"
     CDS             3248..4111
                     /locus_tag="ACG89C_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4900214.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACG89C_001677"
     CDS             4735..5727
                     /locus_tag="ACG89C_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4900215.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACG89C_001678"
     CDS             5743..6627
                     /locus_tag="ACG89C_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4900216.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACG89C_001679"
     CDS             6676..8283
                     /locus_tag="ACG89C_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4900217.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACG89C_001680"
     CDS             8284..9768
                     /locus_tag="ACG89C_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS4900218.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACG89C_001681"
     CDS             9945..10937
                     /locus_tag="ACG89C_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4900219.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACG89C_001682"
     CDS             10989..11858
                     /locus_tag="ACG89C_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4900220.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG89C_001683"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG89C_001683"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4900221.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAZUKR010000064        13781 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000064 DAZUKR010000000
VERSION     DAZUKR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.064"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1012)
                     /locus_tag="ACG89C_001684"
     CDS             complement(<1..1012)
                     /locus_tag="ACG89C_001684"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS4900222.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQ"
     gene            complement(1002..1493)
                     /gene="coaD"
                     /locus_tag="ACG89C_001685"
     CDS             complement(1002..1493)
                     /gene="coaD"
                     /locus_tag="ACG89C_001685"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4900223.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(1490..2050)
                     /gene="rsmD"
                     /locus_tag="ACG89C_001686"
     CDS             complement(1490..2050)
                     /gene="rsmD"
                     /locus_tag="ACG89C_001686"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS4900224.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(2169..2507)
                     /locus_tag="ACG89C_001687"
     CDS             complement(2169..2507)
                     /locus_tag="ACG89C_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS4900225.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(2491..2937)
                     /locus_tag="ACG89C_001688"
     CDS             complement(2491..2937)
                     /locus_tag="ACG89C_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4900226.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(2918..4042)
                     /locus_tag="ACG89C_001689"
     CDS             complement(2918..4042)
                     /locus_tag="ACG89C_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS4900227.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(4063..7491)
                     /locus_tag="ACG89C_001690"
     CDS             complement(4063..7491)
                     /locus_tag="ACG89C_001690"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS4900228.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(7517..8680)
                     /locus_tag="ACG89C_001691"
     CDS             complement(7517..8680)
                     /locus_tag="ACG89C_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4900229.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(8683..8985)
                     /locus_tag="ACG89C_001692"
     CDS             complement(8683..8985)
                     /locus_tag="ACG89C_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS4900230.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(9099..10871)
                     /gene="recQ"
                     /locus_tag="ACG89C_001693"
     CDS             complement(9099..10871)
                     /gene="recQ"
                     /locus_tag="ACG89C_001693"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS4900231.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            complement(11132..12961)
                     /gene="typA"
                     /locus_tag="ACG89C_001694"
     CDS             complement(11132..12961)
                     /gene="typA"
                     /locus_tag="ACG89C_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS4900232.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            complement(13129..>13781)
                     /locus_tag="ACG89C_001695"
     CDS             complement(13129..>13781)
                     /locus_tag="ACG89C_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4900233.1"
                     /translation="NVDRNIQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIID
                     PIDGTLNFVIEGENFCIMLAVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPR
                     PANKSLSEGLLGVNSYLFGHNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYIS
                     NLSPWDYAAGNVLLEEFGMVTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
BASE COUNT         3755 a         3005 c         2363 g         4658 t
ORIGIN      
        1 cttgtactgt ttttactggt acgattttca ttgaggaatt caatttttta gcggcagctt
       61 ttgcttcttc gtagttggtt tttatttcag gatattcttt cttaacatcg tctgaaatcg
      121 tatcatctgg tgcaaagaaa atctctacgc cagctttatc cgcacttgcc acttttttat
      181 cgattcctcc gattcggcca acttctcctt cactgttgat cgttccagtt cctgcaattt
      241 gcttaccatg tcgcaagttt ttaccagtga cttgttcata gatttctaaa gtgaacatca
      301 atcccgctga tggcccaccg atagagccag aattaatacg aacatctgta tctgattcga
      361 tctcggtatg gtcagtaagc gtgataccta ttcctgcctt tttatctgtt ggtaactcga
      421 tcagatcacc agaagctgtt ttttcttcac cattatgcaa ataactgatt tcgattgttt
      481 gtccaacttt ttggcttttt acataatcca taaatgcttc tgtactttga aagcttttcc
      541 cgtctacttt tgttactgta tctccaactg aaagcttgcc aaagaaattc gatgttttct
      601 ctacactcat gacatatacg cctttgaatt ccattttata aggcttgtct gcaagtttta
      661 acgcttgttc gattgctgca ttttgggaag aatccatata atactgttgc atttgattat
      721 attcttcatt tgtcgcccca ccagtcaatt cttcttcact gatcaattct tcaaaagggg
      781 tcattttagc ttttaaagct aagaaacctg tagcttgcct gataccaaca gaggtaagag
      841 agaaagaccc agaaccttta tcttccttat cattgaccgt aattagttct ttcagatcga
      901 tcgtggctcc cggtgattct acataatacg gcaagggaac tactacaaat ccaatgatca
      961 acattccaaa tatgataatc agtactgttt tcaaccattg tttagctttc atttgattca
     1021 cgctttccag ctaaagcttc gttgatcact ggcggtaagt agctgctcac atctccgcca
     1081 aagtgcagta cttcttttaa aatactggaa ctcacgtgac tatattctgg ttttgctaat
     1141 aagaagacag tttccaattc atcatataga tgatgattca tctgagcaat ctctcgttca
     1201 tattcaaagt ctttgatact tcgtatgcct ctgatcaacg catctgcacc aatctctttt
     1261 gctgtttcta ccgtcaattg attttcttga tgcatgactt ttacattagg aagatgagaa
     1321 acagcttttg tgatcaatgt cattctttct tctgggggaa acaaactttt ctttgaagta
     1381 ttgataaaaa caccaattac tacctcatcg aataatttag ccgcacgttc caccgtatct
     1441 aagtgacctt ttgtaaaagg atcaaaactt cctgggaata atgcacgttt cattgatttc
     1501 cctcttttct atagatagtt acggccgtta tgccataaat ctgttttctt atttgatgga
     1561 atccagccac agtttctggc aattcgactg ttttatccgt ttcgcatacg ataactgcgg
     1621 ttttagaaaa aagtcccagt tcttccattt tatggatttg atcgattatt ttttgttttg
     1681 catatggcgg atcaagaaac aagtagtcga attgttgttt ctccgctgct agccgttcga
     1741 ttgctttgtc cgcatcaagt tttattgtgg taaatttctc tggttctttc gtaatttcga
     1801 tgttttcttt gatgattttt agcgcttggt aatttttgtc tacgcatacc gcatgactgc
     1861 agcctctaga tactgcttcg atagctaaac caccgcttcc tgcaaacaga tccaatactg
     1921 tctctccatc aaaatatggt ccaatcatat tgaatatgga ttcttttact ttatctgtag
     1981 ttggtctggt gttcgcccct gctaaacttt ttaaacggcg cccgccataa tttcctgata
     2041 ttatccgcat ctactcactc cttagaaatg gtactggtac tagtatacca aaaaaatact
     2101 tggacaatga tcaaaaatcg atcgttatcc aagttatttt ttatttaaaa actgctcata
     2161 agagattttt aaagatgttc agctaatcgg atttctgtat ttagctcttc aatttcgaaa
     2221 ccttcgtctt ttaatactgt tttgtttcct attttttcgg caaaattcat ttcgatatcc
     2281 ggtcggtaag aacgttctac gtttcgaaca aaatgtaatt tacggattcg ttcttctgtt
     2341 tcttccaatg tctcttcatt taagtaaagc acgacatatt tcattcttct tgagacataa
     2401 tgcaacaagc cgaacttttt cagattcttt aattgtttta agctgtagac ccatacgatc
     2461 agcccgcgtc gttttgttat ttcaaattca ctattgtcta attgcatgac aattacctcc
     2521 gcatccaaag tgttttttcg tttcaaaaaa cgggctacct gcatctactt tgatttcatt
     2581 agaaacttct tcggcaagta cttggacaat ttgatccaac agactttgca gctgtgtttc
     2641 actatatcgg aaagctgcaa catgctcatc catatcaagc tgtctttttg cttttcgaac
     2701 agctcgtttt ttttcacgat aatcaggtgc atactcacca tatgccgcta cattttcaaa
     2761 tgcttctttc ttagacaaga acgcttcttt caactggcaa acttttgcag attcttccat
     2821 tgactgttta tgttcaagat aagaagccat cgttttactc ttttttatca tttcagcgac
     2881 ttggaggcat tgatcctcca gttgaaacag ctcttcgtta acgatcactg ctatcactac
     2941 tttctccgtt ttcttcttgc aataagacta acatattttc tttagaaaaa gcaatgccta
     3001 cagaatccgt tcccgggtcc atgaaacggt catggttgta aggatcgctg taccaatata
     3061 aaaacgaaaa agtagcgtca atcgccggat gaataaatag cccactagta ttttctaatt
     3121 tttgtttact gattaacttc tccaattcat cattcgtcag tgagaatttc actgtcgttt
     3181 gagcactgcc tagcgatttt tgccaatcag ccatacgttc ctctgacaat atttcctcag
     3241 gccttgtcaa aaaatcatgt agtagcagct gactgtggtt ttgaaaggca agtgtttgat
     3301 tgtatatggg aagttcgtct tctcttctaa gcatatttaa taaagtcgta ctttttcgtt
     3361 ctttaacgcg attgattgat tcccaatcag ccgatttatc tgcttgataa tgtggaaggc
     3421 cttcaccaaa cacttgatat ggcaatagtt tcaaaagact atctttatct aaatacacaa
     3481 ctgaaaatag gccgtttgtt gtttgatcaa aaaataaaat agcaaaagtg tcgttatcaa
     3541 aagcaattag cggacgataa ttcatatcct cttccattag ttcgattcca acttctgtat
     3601 catggtactc aaaagtgaaa ttagtataga tagtagtgat tttcgtaaga tcttgcatcg
     3661 tcatccctaa ataaaacggc gcgatatcag caggccctgt ttgcagtact ttaacagttg
     3721 tgactctgct gtcttggatg ttcgcttcaa atgtgagatg actagcttta ttttcataat
     3781 atcttgtttc aaaaccaaat ccgctagtta atcgatcata ggcttggcca aaatcctttt
     3841 ccagctgatc gatcggtttt ccgatataag tagaaaaacc agttgcttta agctcctggt
     3901 atttccacgt tgtcatttgg tttgatattt gttgatcaga gttttccttc acttcaagtt
     3961 ttggcggaaa caagactggt tccatgtaga atgccacaat aactaatagc agaatcagcc
     4021 aaaacaaacc cagtcttttc atgtttcccc acctttcgct tattatttct cagtcacttc
     4081 aatcaaaagg tcaccagacg aaatagtgtc tcctgaagac acatatacat gtgcgacttc
     4141 tccgtcaaaa cgtgcttcga tggccgtttc cattttcatc gcttcagtaa tcatcaacgt
     4201 atcacctttt ttaacgttat cgcctttttt cactaataca tccaaaacag aaccagacat
     4261 cgttgctcca atttgttctc ggtttgtcgg ttcagctttc aatcgggttt ggaccgtact
     4321 gatgatcgaa ttatctttga tagtgatttc acgtctttgt ccattcaaat tgaagaacag
     4381 tacgcgattc ccttcgatat cgggttctcc gatttcatct aaacgaatga taagcgtttt
     4441 tccgcgttcg atttgtacat tgattgtttc acccaaacgt ataccttgga aaaatgttgg
     4501 tgtgtccagt aatgtaacat cgccaaattg attatatgct ttttgatagt ccaaaaagac
     4561 ttgcggatac atcaaatagc ttaatacttc gtcttgtttt ggttcatagc caattttttc
     4621 tgccaattct ttttttactt cgttgaagtc gactgattca gccaaaagac ctggacgttc
     4681 attcaatgca ggtctgcctt taagaataat cttttgcaat tttttaggaa atccgccaac
     4741 gggttgccct agttctcctt ggaagaaagt cacaacagat tcagggaaac ttagtgtttc
     4801 gcctttttca taaatatctt cttctgtaag atcattttgg accataaata gtgccatatc
     4861 tccaactact ttagatgatg gagttacttt aacgatatcg ccaaacatca agttgacagt
     4921 atgatacatt tgtttgattt catcccagcg atgacctaat ccgacagctt ttgcctgttg
     4981 ctgtagattc gaatactgtc ctcctggcat ttcatgcata tatacttctg tttcaggagc
     5041 attcaagccg ttttcaaacg gtttgtaata catgcgcaca tcttcccaat aatggtttaa
     5101 tttctgtgca ttttcaatcg tgatttctgg cagccgtgga ccattgacca gtgcataata
     5161 caagctgctc atgctaggct gactagtatt cccactcatt gcactcattg ccacgtcaac
     5221 gatgtctact cctgcttttg aagcagcaga ataagtgatg attccattcc cgctggtatc
     5281 atgagtatgc agatggattg gcagatctgt cgtttctttc aattcactaa tcaaacgata
     5341 agctgcctgc ggtttaagca gcccagccat atctttgatt gcaatgatat gcgcacccag
     5401 ttgttctagt tcctttgcca tgtctttata atactgaaca ttgtattttg cccgacttgg
     5461 atcgttgata tcgccagtat agcaaatagc tgcctctgcg attttaccag tatcccgtac
     5521 agcttggata cttttttcca tttgtggagt ccagttcaaa ctatcaaaga ttcgaaatac
     5581 atcgattcct tgcgctgctg cttctttgac aaattccacc aatacattgt caggataatt
     5641 agaataccca acggcatttg aacctctgaa aagcatttgc agcaacgtat taggcattaa
     5701 agaacgaatt tttctcaacc gttgccaagg atcttcattc aaaaagcggt aagccacatc
     5761 aaatgttgct cccccccaca tctcacttga aaagagttca ggaagacctt ctccagtcaa
     5821 acgtgcaatc tgcttgaaat cctttgtccg aacacgagtt gctagcagac tttgatgggc
     5881 gtctctaaat gtcgtatccg tcaacaacaa attttcctgt cctttgatcc attcgaccaa
     5941 agcatcggca cccttcgcat ctaatacatt cttagccgtt acgtaatcag ttcttgtgat
     6001 tagatcttta ggcatacgtg gttcttcata aaatggtttt tcgccacttt cgatacctgg
     6061 aaaaccattc acagtaattt caccgatata tttcatcgtt ttgtttccgc ggtctcgtag
     6121 tcgaggaaat tcaaataatg ttgctgtact atcaatgaag gttgtttttg catttcctga
     6181 ttggaattca ggatgtgtga tgacattgag cataaatgga atgtttgttt tcactccacg
     6241 gattctaaat tctttcaagc aacgttccat tttctgaatg gctgtttcaa aggtagcacc
     6301 atgcgtacat acttttacta gcaatgaatc aaaatacggt gtcactacat agcccgcgta
     6361 agcatttccg acatccagac gtacgccaaa accaccaggt gaacgatacg tatcgatttt
     6421 ccctgtgtct ggtaaaaagt tattcaacgg gtcttctgta gtgattcggc attgaatggc
     6481 tgaaccattt aatttaattc cttcttgctg aggcaatccg atttctttat gcagatccaa
     6541 accttgagca atcaacagtt gcgttgttac gatatcaata tctgtaatca tttctgtgat
     6601 cgtatgttcc acttggacgc gagggttgac ttcgataaag taaaaatcat ctccttcaac
     6661 aagaaattct actgtacctg cgttgacata cccgacatat ttcatcaatt ggacagctgc
     6721 ctggcagatt ttttgacgtt gctgttcatt catagagaca caaggcgcta cttctactac
     6781 tttttggtga cggcgctgaa cagaacaatc tctttcaaaa agatggatga cgtttccatg
     6841 cgtatccccg agaatctgta cttcgatatg cttaggatta gcgatgtatt tttcaacata
     6901 cacttcatca ctaccaaagg ctgcttttgc ttcgcttttc gcacgttcgt agccctctct
     6961 tgcactcttt tcatcgtgag ctacacgcat gccgcgtccg ccgccgccta gagctgcctt
     7021 gatcataatc ggataaccgt attgtttcgc aaactccaag gcatcgtcta tagaagcgat
     7081 tggtccatct gtgcctggaa tcgaagcaat ccccgcttcg atagctgcag cttttgcttt
     7141 gattttatct ccaaaaatat ctaaatgatg cagtttgggt ccaacaaaaa tgatgccttc
     7201 ttcttcgcac cgttgcgcaa aattcaagtt ttccgacaaa agaccgtaac ccggatgaat
     7261 agcgtcagct ccgcattctt tcgcgataga aatgatccct tcaatatcta gatacgcatc
     7321 aatcggtttt tttccttgtc ctactaaata tgcttcatcc gctttgaaac ggtgtacgga
     7381 atattcatct tcttctgcat agattccaac agttttaatt cctaattctg tacaagcacg
     7441 aaatacacgt acagcgattt cacccctgtt agcaactaaa acttttttca tcgaaccact
     7501 cctacatctt tttttattat aaacctaagc tcttgcgttt ttcatccgca ctgatgttga
     7561 ggacaaagcc gacacaaatc gaaagcatca gcaaactgga gcctccttga cttaaaaatg
     7621 ggaacgtgat ccctgtcaaa ggaataacgc cagtaattcc tccaagattg acaaatactt
     7681 ggatcaaaaa cattgcgccg atcccaatgc acaaaagcga attgaacgga tcttttgaac
     7741 ggatacctac aaggacaatg cgcacaatca taaagaatag caacgctaat atcaaaatcc
     7801 ccataatcac tcccagttct tccactacga ttgcataaat aaaatcggtt tgtgcttctt
     7861 gtaaaaatcc ttttttctgg atactgttac ctaatcccct gccaaatagt cctccgttga
     7921 acatggcata gtagccgttg actagttgat gccctttatt taattcgtct gagaaaggat
     7981 tttggtaaat agcaaatcga ttatagatat actgtaaacg tccgggtaag atctttccgt
     8041 ttgtgatatt gatcagccag atcgtaaaag tacttagaca aaagcctcct actcctacaa
     8101 tcaaagtata cacataattg actccgctgg ctaaaagcag aaccaaaata atcaaaaaga
     8161 taactgccgc attaccaaaa tcgggaagaa tagcgactaa tgcagtaagt ccgataacca
     8221 ttgcaagtgg acgtttcaca gtcccaagaa aatctttttg tacactgttt tgtcttttag
     8281 ataaagtcag agacaggtac cagatagaaa tgatttttag gtattcggca ggttggatcg
     8341 agaatcctgc aatttgcagc caacctttcg ccccattgat ctccttacca aaaaacacaa
     8401 caatcagcag caaaatcgtc aaaactgcga tggctgccat gatcaaacgc tggtttttca
     8461 aaacatctgt tttcatttta taaagtagtg cgatagcaat caagctaagc acccaaaaaa
     8521 tactctggtt aatgactgat gcggacggat tttgtccgtt ttctaataat agatacgacg
     8581 tagaactata taccatgatc aatcccaata cacataaaat taaataggga atcaagatgc
     8641 tatagtctaa tagatgcctt tttttcactt tcttaggcaa gtctattcct ccttgccaac
     8701 ttctttttta tcataaattt cttggtaaac ctcactatat aactgattca attctcgttc
     8761 tagctcactt aacatcagct ggccctcgtt ctttccgatt actcctatac ggacagcaaa
     8821 accgacttgc cgggaaaagc cgtacatctg tgtatctacc acttcttcga aggctttgca
     8881 ttgtgagata cagagactgt tgttttgatt tcggattaat cgtttgatcc gttctgcttc
     8941 atcatttaat acttggatcg caagtttttt cgacaaagaa tccatctgtg cttacctccc
     9001 attctaattc cttaattata acatatttga ttttcataag gagcaaaaaa aacagattta
     9061 accagttgtt tttattcatt tttacgtatt cgttctttct acttatccag aacgttagag
     9121 tccgcatttt ttatccgaga taaaaaggct tcgccatatt tatcaagctt gctttggcca
     9181 actcctttta tctgaagcat ctccaatctg cttgatggac gtattcggct catttctttc
     9241 aatgtggaat ctgaaaatac gacatacggg ggaacgcctg cttctgatgc tagatccgtt
     9301 cgcagttccc gcagcacttc aaatagtgta tcggtttctt catccgatag ctgctggact
     9361 ttcttgggtt cctttcgata aacagattct tgtcctttca atacttgtat ccctctttct
     9421 gttactttca aaacaggata ctcaccattg atggtcagaa gatagccatt cgatatcaaa
     9481 tattcaatta actgcatcgt ttctttttgc gattggtttt tcatcagtcc ataggtagat
     9541 aaatgtccaa aacctaaagc ttgcagcttt tgttctttcg atcctgctaa gactttcatc
     9601 agcatttgtt taccatagtt ctcacccata cgttttaagc aagaaagaac catctgtgct
     9661 tctgtcgtta cttctactat ttctctgtca tctaaacagt taccacaacg gttgcacgga
     9721 ctgcttgtct cgccaaaata gtttaggatg tattgctgaa gacaggactc aatatacacg
     9781 tattctgtca tcgcttttaa tttttcatat tctttttgct tttgcccttc ttctctttgg
     9841 gactgctgga taaaaaactt ttggacttgg atatcttgag gagagaaaag caggatggct
     9901 tcactggaaa gaccatctct tcccgcccga ccagcttcct gatagtatgc ttctagagag
     9961 ccaggaattt gataatggat aacaaaacgg acattgcttt tatttatacc cataccaaat
    10021 gcatttgtcg ctacaatcag ctgcaagcga tcgtataaaa aggcttcttg catttctgtt
    10081 cgctcattct catttagacc accatgatat ctacccacgg aaaaatcgaa ttttttaagc
    10141 aaatgataga gacggtctac ttcttttctc gttgctgcat aaatgatacc agattgattt
    10201 ttgttgatct ttaaatattc gattagatat tgttcttttt tttgatcttt aattacttga
    10261 aatcgtagat tttctcgttc aaaaccagtc tgaatatggt tgttttctgg aatttttaaa
    10321 agtcttttta tgtcagcagc tacttgcaca gtcgctgtag ctgtcaaagc aacaattaca
    10381 ggacgatttg gcagttgatc tagctggttc gccatctgta aataacttgg gcggaaatca
    10441 tgcccccact gagagataca atgtgcctcg tcaatagcga tcatacggac gttccatcgc
    10501 ttcattgctt ggataaaatc cggcatgata aaacgttcag gagcgatata aagtaaacgg
    10561 tactgctgtc tatcgatttc ttggaagcgt ctagctgttt cgtaaccatc taacgagcta
    10621 ttgataaaag tggcgggtat acctaattga ttcgccgcgt ccacctgatc cttcatcaaa
    10681 gagattaaag gggacacaac cacagtaagc ccatctaata gtaaagctgg aagctggtaa
    10741 cagattgatt tcccacttcc ggtaggcatg attcctaata cattttcctg atcaatgact
    10801 ttctggataa tttctttttg accaggtcta aactcatcat aaccaaagta ttgttttaat
    10861 aagtcgttca tcttcccgct cctttctctt gccattttac ttcgattttc ttcgaaggac
    10921 aagttgatct cctttcgtga aacattatct tattatgaaa caaaaactct ctagaaataa
    10981 gttttttaaa agtcttttaa acgtctttcg gcgtattttc tgaatttatg tccattgaca
    11041 aagatgactt ctccacagaa aagagttgga caaaaaaaga aaccagctat tttttataac
    11101 aagctggttt ctctcttcat ccttttacag attatttttt ctttttagat gctttttcac
    11161 gagcattttt ctctaaaatt tgttttctca agcgaatgct ttctggtgtt acttcgcaat
    11221 attcatcatc attcaagaat tccaaagatt cttccaacgt caattgtttt ggttttttga
    11281 tgacagatgt ttggtcttta gtagcagacc gtacgtttgt catttgtttt gctttcgtga
    11341 tgttgacagt caaatcatta tcacggctgt tttccccgat aatcattcct tcatatactt
    11401 ctgtacctgg ttctacgaag actgtaccgc gttcttcgat actcatgata gaataagtcg
    11461 ttgcttttcc tgtatcgatc gaaacaagcg ccccttggtg acgtccgcca atttgaccag
    11521 gaagcattgg cagatattgg tcaaaagtat ggttcatgat tccgtaacca cgtgtcattg
    11581 aaaggaattc agttgaataa ccaatcaaac cgcgggcagg tgttaagaat gtcaaacgga
    11641 tttgtccgtt tcctgtatgg accatatctt gcatttcacc tttacgtaag ctcaaggact
    11701 cgataacact acccatatat tcttctggtg tatcgatttg cacgcgttca aacggttcgc
    11761 attttacacc gtcgatttct ttttcaataa cttctggacg agatacttgc aattcgtatc
    11821 cctcacggcg catgttttca atcaagatag acaaatgcaa ttctccacga ccagaaactg
    11881 tccatgcatc tggagagttt gttggttcaa ctcgtaagga tacatctgtt tgaagttctg
    11941 ccatcaaacg ttcttcaatc ttacgagcgg ttacgaattt accttctcga ccggcaaatg
    12001 gtgaattatt aactaagaaa gtcatttgca atgttggttc gtcaatgtga aggataggta
    12061 acgcatcttg atgatccaca ggagtaactg tttcaccgac aaagatatct tccatacctg
    12121 agacagcaat caagtctcca gcttttgctt cttgaatctc tagacgtttt agtccaaaga
    12181 aaccaaataa tttcgttaca cggaatttct taactgttcc gtctaattta attaatgcta
    12241 cttggtctcc tactttgatt gttcctctaa atacacgtcc gattccgata cgacctacat
    12301 aatcgttgta gtcaagtaat gaaacttgga attgtaatgg ttcgtcgctg ttatcgacag
    12361 gagcagggat tttttcaata attgtatcaa aaataggtgc catggttggt tcttgatctg
    12421 ctggatcatc tgacaagctt gacgtaccat ttagtgcaga tgcataaatc acaggaaagt
    12481 ctaactgatc atcatctgct cctaattcga tgaatagttc aagaacttca tctacgacat
    12541 gctcaggacg agcagaaggt ttgtcaatct tgtttacgac aacgattggt gtgatatgtt
    12601 gttccagtgc ttttttcaat acaaaacgtg tttgaggcat tgttccttca tacgcatcta
    12661 cgaccaacac aacgccgtca accattttca tgatccgttc tacttcgcca ccgaagtccg
    12721 cgtgtcctgg agtatccatg atgtttacgc gaataccttt atagtctaca gctgtgtttt
    12781 tggctaaaat cgtgatacca cgttcttttt ctagcgcatt tgagtccatc gcacgttctt
    12841 gcaattgcgt atgtgcgtct aatgtatctg attgttttaa tagttcatca actaatgttg
    12901 tttttccgtg gtcaacgtgg gcaataattg cgacgttacg gatatcgttt ctataattca
    12961 atgtaactaa ctcctttttt attggttctt tatgacaggg caaattttac tcgactgcta
    13021 attatagcag acttttttca tgaaatatag taaaatgttt tcattttttc gagaaaatta
    13081 atcttttgct cgtttttatc atttttgtga atgatttcta cctttttttc agccttcttt
    13141 tttcaaaagg tctgcgattt ctgcatatgc acaagaagtc cctgctaaat agtattccct
    13201 tttttcaaat gtcaaaggag taccaaaaag accgcttgtt accataccaa actcttccaa
    13261 taagacattt cccgctgcat aatcccaagg gctaaggttt gaaatataag caatgtggtt
    13321 tcctttcaag attgccacta gttcaagacc agcgcatccg tacatacgtg tccccatgct
    13381 gctttccgca atttctcgga ttccatattt attatgaccg aaaagataac tattcacacc
    13441 aagtaagcct tccgataagg atttatttgc tggtctaggc aatggctcat catttttata
    13501 cacgcctatt ccttttccac cccaaaaaag ttcatttctc atcacgtcat aaatgaaacc
    13561 aagctttcca acgccggatt cataaacagc gagcataata caaaaatttt ccccttcaat
    13621 cacaaaattc aaggtgccat caataggatc gatgataaag actcttccat cgagatccga
    13681 taattgatta cacccttctt cttctgccaa tattttcgcc tctggatcaa aggaatggat
    13741 tttttcgatt agaaaattct ggatgtttcg atcgacattt g
//
LOCUS       DAZUKR010000065        13704 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000065 DAZUKR010000000
VERSION     DAZUKR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.065"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..239
                     /locus_tag="ACG89C_001696"
     CDS             45..239
                     /locus_tag="ACG89C_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900234.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            508..807
                     /locus_tag="ACG89C_001697"
     CDS             508..807
                     /locus_tag="ACG89C_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900235.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(904..1338)
                     /locus_tag="ACG89C_001698"
     CDS             complement(904..1338)
                     /locus_tag="ACG89C_001698"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900236.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1570..2280)
                     /locus_tag="ACG89C_001699"
     CDS             complement(1570..2280)
                     /locus_tag="ACG89C_001699"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4900237.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2270..3130)
                     /locus_tag="ACG89C_001700"
     CDS             complement(2270..3130)
                     /locus_tag="ACG89C_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4900238.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3135..3773)
                     /locus_tag="ACG89C_001701"
     CDS             complement(3135..3773)
                     /locus_tag="ACG89C_001701"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4900239.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3788..4087)
                     /locus_tag="ACG89C_001702"
     CDS             complement(3788..4087)
                     /locus_tag="ACG89C_001702"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4900240.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4130..5614)
                     /locus_tag="ACG89C_001703"
     CDS             complement(4130..5614)
                     /locus_tag="ACG89C_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4900241.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5634..6095)
                     /locus_tag="ACG89C_001704"
     CDS             complement(5634..6095)
                     /locus_tag="ACG89C_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4900242.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6113..7180)
                     /gene="ulaG"
                     /locus_tag="ACG89C_001705"
     CDS             complement(6113..7180)
                     /gene="ulaG"
                     /locus_tag="ACG89C_001705"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4900243.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7398..8168
                     /locus_tag="ACG89C_001706"
     CDS             7398..8168
                     /locus_tag="ACG89C_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4900244.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8441..9268)
                     /locus_tag="ACG89C_001707"
     CDS             complement(8441..9268)
                     /locus_tag="ACG89C_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900245.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9836..11071
                     /locus_tag="ACG89C_001708"
     CDS             9836..11071
                     /locus_tag="ACG89C_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4900246.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12070..12330)
                     /locus_tag="ACG89C_001709"
     CDS             complement(12070..12330)
                     /locus_tag="ACG89C_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4900247.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12357..13658)
                     /locus_tag="ACG89C_001710"
     CDS             complement(12357..13658)
                     /locus_tag="ACG89C_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4900248.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4398 a         2815 c         2167 g         4324 t
ORIGIN      
        1 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
       61 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      121 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      181 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      241 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      301 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      361 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
      421 caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt atttttataa
      481 tgtatcagta tagaaaggaa ataaaacatg gattatactg ttgaatttga cagtgaacta
      541 cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg
      601 acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa
      661 ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta
      721 aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat
      781 aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa
      841 gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat
      901 atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat
      961 accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc
     1021 agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc
     1081 ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc
     1141 ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc
     1201 ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt
     1261 gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat
     1321 aagggaatca atattcattt ttttcttcct ttcttataca attctccctc taataataca
     1381 gttctttgac tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt
     1441 aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt tgtgataaat
     1501 tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc tacccttacc
     1561 ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat aatgtttatc
     1621 tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt ctttggccat
     1681 cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg aatcccccca
     1741 tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat ctaattttct
     1801 ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt ctcttacttc
     1861 ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag catgagtcgt
     1921 gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg tggaatgagt
     1981 atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg tggggagatc
     2041 tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg ttaccaccat
     2101 ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga tgactcctag
     2161 ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt atcgcggcaa
     2221 tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt cacaagacat
     2281 catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg atttctcttc
     2341 gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca taatctagac
     2401 gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa ggaacatcac
     2461 gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg atagcagtga
     2521 tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat gcggacaaat
     2581 tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa
     2641 attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga atgatgccat
     2701 atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct ctggaattca
     2761 ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg acgcttagat
     2821 tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc ttttctggat
     2881 cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg atacgtacac
     2941 cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg
     3001 ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc atttctttta
     3061 caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca taaatcccaa
     3121 tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt ttgtgcggcc
     3181 ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata cacatcgatc
     3241 ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac attaaagcct
     3301 agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc accagataac
     3361 agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg ccaatccatg
     3421 gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat ttctttttgt
     3481 gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc tgctcctgct
     3541 tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc agcgactagt
     3601 tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa
     3661 agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac atctcctaat
     3721 gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct catcttagtt
     3781 ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca ctgatttctt
     3841 tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct gcctgagtaa
     3901 acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct gaactttttc
     3961 cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct ttcattgctt
     4021 tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac gatactaaaa
     4081 ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt tatactctct
     4141 gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca ggatttttcc
     4201 gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt ccagcataac
     4261 ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg aacatcccga
     4321 tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa ccgcctactt
     4381 gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct cggttattgg
     4441 cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct atgacgatcg
     4501 ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca
     4561 agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct acgtcaattc
     4621 ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga gttagttcat
     4681 cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa tttaaagaag
     4741 tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct tgaatcaaat
     4801 aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt aacgaagtag
     4861 caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct tcaatttttt
     4921 tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac tcagctaatt
     4981 ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg gcaccttctg
     5041 tcaattcctg cgtgattcct actgttaagt tagaagctac tgtccaataa caacctaaaa
     5101 tcagtcccat aaaaatcaat gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa
     5161 tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca
     5221 gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca atcagtaata
     5281 aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca tccacagcat
     5341 tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc tttagtccaa
     5401 ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa ataaagtaac
     5461 cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc agcaaaatat
     5521 agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata tttgttgcaa
     5581 aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat caattaatta
     5641 tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag acgctcaacc
     5701 aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg ttcttctgga
     5761 tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc cccttcactt
     5821 tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa aacaccatca
     5881 cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata aggaccgtat
     5941 ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc cttatcgatc
     6001 aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt atctgggtaa
     6061 tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt tcttataaaa
     6121 acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct cgataaaaat
     6181 taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact tgccagatat
     6241 aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca gtgatctctt
     6301 ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact acttttgcat
     6361 tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt atccctcgtg
     6421 ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca tgtttggcaa
     6481 ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat gtttcgaata
     6541 aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat acctcttctg
     6601 gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact acattgatgt
     6661 ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca ggaattcccc
     6721 atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca ggacaatttt
     6781 gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt gtaacgatta
     6841 aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta cgtaaattag
     6901 gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc atcatgccat
     6961 tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc gttccttcat
     7021 gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt ccaggatcca
     7081 ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat gtttctacta
     7141 accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc cccccatatc
     7201 gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa cccttttata
     7261 tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc agtataataa
     7321 agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat caagatgtcc
     7381 ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa
     7441 ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct gaatcccttg
     7501 atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa aacgagatca
     7561 tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt gatcaatctg
     7621 gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt aacgattatg
     7681 atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt cttcaatcta
     7741 accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac aaagataatt
     7801 gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta gtgggcgaca
     7861 tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc tgtagcggag
     7921 tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc aacgaaatga
     7981 tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa atcggactga
     8041 catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact gataaatttt
     8101 gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt caagtagatt
     8161 cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg aataacaaat
     8221 ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca agatgtatcc
     8281 atccaatcga taccatcata atctattgct tcttttataa ccgttatatg ttgcctaaat
     8341 aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa
     8401 taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg tcgttttcaa
     8461 tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg tccatatatc
     8521 tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca taaagcaagt
     8581 attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc tcacataatt
     8641 ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca taatatacaa
     8701 tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta cgctccacat
     8761 aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg tttgcagctg
     8821 cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta aaataaaata
     8881 atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa aataaaattt
     8941 gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa attttataag
     9001 attcttggta ttttgataca gaattcaatg attgaatctc atttattttt ttcctgattt
     9061 tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt ttgcaaagcg
     9121 cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg acagttgctt
     9181 gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt cggtattctt
     9241 ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaaaa aattatttct
     9301 cctttatatc atacacaaaa aaaagttcta aacaaccaaa aatgtttaga acagaataaa
     9361 aattttaatt ttaaaataat attcaaatta aacaactggc gtatctgctc cttgatttct
     9421 ttttgtgtta tctgaataat tatcgtttac agttataaca catagtgaaa attgtcatgg
     9481 cgagaaagtc taagaatatt tttaaacata actatttctt tcttacgtgt gtataatgac
     9541 gacagcacac ctatcaatta atataattat tttatttttt tatgttctca tgtttatttt
     9601 taatgacaca acttctcgta tttatctggc acaacatata aatcgatcaa aaaattgttg
     9661 cgtgtttttt tgaaaattgg agtttcaact tgattacaaa atttaaattt agaagttgtg
     9721 aaaacagcga gagacagttt aatattcaaa atatacatag gctactcgaa cgctgtgttt
     9781 tttagtatac tagtaagaaa cgaagggata gactcattcc aataaggagg atttcatgaa
     9841 gacaattctt tttgctcctg cagtattcaa tttagctgag acaacacgaa tgattgaggt
     9901 tgccaaagct atgaaagacc gttattactg tgtattcttt ggcttttcta aaacttttgc
     9961 tcctttgatc acagaaagtg gctttgagta tcatttacta gcgccgatcc ttacgaaaaa
    10021 acaagaagaa caaatcataa aatttgacca gttgcgtaca cttaaaaacc cttttaccaa
    10081 agaaatggtt gcctctcgta tcactagtga acagagtctg attgctaagt ttgagcctcg
    10141 actgattgtg acaggttcga atgtgacaat ttttttatcg gcccgcaata aaaagattcc
    10201 tttagtatat gttaaaccct atgctttgag tgaacctttt tttcaatcag agcatgcgct
    10261 gatgccccac gagttacgtt tcttcggccc tttaagcaat cccctttggc actggacaaa
    10321 aaagcgtata gtaaataccc gatggatccc caatgccttc aaacacgctg cacaaaaaga
    10381 aatgcttcct ttaccaaaaa caagtttaca aatgatggat gctgatctaa atctcatcac
    10441 aacgcctaaa ctatttttgg aagaagaaca gctgccggaa aattatcgaa tcgttggtcc
    10501 aatttttgct aagctcagta cttccttgcc tgaagaagtg ctaactttta tacagcgaaa
    10561 gcaacaagaa agaaaacgta ttatctattt cgcaatgggt agttcaggca atccgcgtat
    10621 gttacgcaaa attttgttct tcttacaaaa acaaccggat ctagcggtta ttgcacctat
    10681 tgactatctg cttgatgaca ttcaatctga ctcagaagat cttttaataa cagaatatct
    10741 gtctagtcat cttttgcatg aattcattga tttttctttt atccacggcg gggaagggac
    10801 cgtccaaact gcctgtgaat ccggcaaacc gtttatcggt gtggggatgc aatttgagca
    10861 aagctgtaat attgactatt gtgtaaaata cggaaacgca atcgccctca caaaaccagt
    10921 gactgaaaga aaactgcaga ttgctctgcg cgaagtttct tcacccaaga ttcgtcagca
    10981 ggcacgcacg ctgcaagccg tttttccaat aaatggtcca caacaagcaa ttcaagaaat
    11041 cgaaaccttc ctgagcaacc acccaatata gtttcaagta gaaaaagctt ttttaataga
    11101 gactcatcag taatttcgct attcgagacc cagacagtga aaatacaaaa aaagtaaata
    11161 gtggattgaa aagttagagt tacttaaatt acggtgtcta aaaaacacaa aatcatcctt
    11221 aaaataaaag atctgacgct atccaaatga gcataggtag cgtctgccat ttattcttag
    11281 tcaaattctt tgacaccttt ctctggttgg atcatctcaa cgtttcttca aaagaaagac
    11341 cataataatt aggcaaatac attttcttaa aatgtgtcat atagatctag aagcatcgct
    11401 ttgtagagat ttgtgccgct agtcccctca acaattacta aatcaaatgt aggatttacc
    11461 actttatctt tgataatcaa cctccctctt ctgttgagac aatgatctcg caatccgatg
    11521 catactccta tataaactgc cagcaatccc cacatattga gtagatcatg tcagaatgtt
    11581 ctatgtcgtc agcaacgata cactaaaagt cacgttcacc atgactgatt gctattccca
    11641 gcacaattat ttctatacac gtttcatgat ttgaataaac actcacatca tacatattac
    11701 cattcataca ccatactgca gcccctacta tatggttgta agctcccaaa tctatatttg
    11761 acttgatcac tttggtagta gatctactaa ttctaaatcc attgctgata aaatttccat
    11821 tttttcctcc ttctttctgc caaaacagtt aaaacaataa taatctttat gtaaaaaagg
    11881 gctgtgacaa aagccttgtc acagtcccat attccgaata aacggtggtc aaaagcttgc
    11941 atctgcggta ataagttcaa ccaaacgaaa aataagccaa catttcacaa aaatttaaga
    12001 agcaattttc gtaaatttct tcttatttct tgaagctaac cacttctgtt acaaccactt
    12061 ttagaacatt taaagcttcg atgtgacaaa ccatctatgc tgctcgatca taaaattgat
    12121 agcaaaaatt ccacaaagta catatgtccc ccatgaaaac aaaacattca tatgcggtac
    12181 aatgatcttt ggaagaatca cgatcaaaaa tgatgccagt gcccaaatcc atcgattgat
    12241 ttgaatatga tatgtagcta gcatattcaa tataaggtga ctagccgtta ataaaaagat
    12301 aaaaacaaaa aataaaatca gccaatacat tgtttcccac cttctaaaaa ccaaacttag
    12361 acagtctgga cttcgttttc tccacgactt tgttcatctg acagcatctt tttatcataa
    12421 gcttttacaa atggataata gataataaat gctatcaatg tgttgatcat gatcaagata
    12481 acaacacgcc agtcatttcc tgctgaaaga taaccgccga ttggagctgg taatgtccat
    12541 gttggcaaaa cagtaaatct attgaccatt tctaatttga ctgcaaaata actgataata
    12601 gtcaaaacta aaggaactaa gttgaacggg atgaacatgt aaggattcaa catcatagga
    12661 gctccaaaaa ttatgggctc attgatattg aagataccag gaataataga aaaacgacca
    12721 acctctttca agaattttga tttacagaag aatataaaca aaacagatac cacgatcgtt
    12781 gctcctgcac caccaattgt taccgtccat tgataaaact gttcaggagc gatatagggt
    12841 aattgggtgg cagctgtacc atctgccaat gcctttgcat tctcatccaa catgatcagc
    12901 cacattggac gaacaacagc ccctacaata ctcatgccat ggataccaaa tgaccacaac
    12961 aaatggatca aaaacattgg tagtaaaaca ccaagcaaat tattccctaa aatattggtt
    13021 aaaggctcga ataacgataa taaggcagcg tttacatcaa agcccgcgac gcttcgaatc
    13081 agccaagtaa ttgttaaaat gactgcacct ggaatcaatg cctcaaaact acgggcaaca
    13141 gcaggtggca cttgttctgg cattttgata gtaatatttc gatttttaaa gaaacgatat
    13201 acttccactg caaaaatcat agataagatc cccgcaaaca tcccagatgc tcccaaagaa
    13261 ctcataggta agacccagcc aagaggtgcc tcttcaggag ttaaaacgtt tacaggaact
    13321 tgtaacataa agaacgttcc taaagataat ataccacctg tcacactatc taatccatat
    13381 gatttagcta gatccgaacc cataacaaaa gttgcataaa tagccatcaa tcccatcgtc
    13441 atcctaaagg gtaaaacaat cgttgccgaa taaggggcaa taaattcatt ccatccagga
    13501 aacggtaaat tgataaagac cataaaaaat gtcccaatca aaatcaatga caatgttgaa
    13561 acaaccccat tacgaattgc taataaatga cgctgtgtac caatttttgc cattggtggg
    13621 acaagtttct cttcgaccca tttcatcagc ttttccacta aaaccactcc ttttcaataa
    13681 aatatgaacc attcattgca tgac
//
LOCUS       DAZUKR010000066        13688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000066 DAZUKR010000000
VERSION     DAZUKR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.066"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..477
                     /locus_tag="ACG89C_001711"
     CDS             139..477
                     /locus_tag="ACG89C_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900249.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="ACG89C_001712"
     CDS             complement(682..1257)
                     /locus_tag="ACG89C_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFS4900250.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="ACG89C_001713"
     CDS             complement(1989..2570)
                     /locus_tag="ACG89C_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4900251.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="ACG89C_001714"
     CDS             complement(2753..3379)
                     /locus_tag="ACG89C_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4900252.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="ACG89C_001715"
     CDS             complement(3401..4732)
                     /locus_tag="ACG89C_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS4900253.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="ACG89C_001716"
     CDS             complement(4887..5288)
                     /locus_tag="ACG89C_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4900254.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="ACG89C_001717"
     CDS             complement(5374..5580)
                     /locus_tag="ACG89C_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900255.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="ACG89C_001718"
     CDS             complement(5675..7177)
                     /locus_tag="ACG89C_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS4900256.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG89C_001719"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG89C_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4900257.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="ACG89C_001720"
     CDS             complement(7748..8365)
                     /locus_tag="ACG89C_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4900258.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="ACG89C_001721"
     CDS             8586..9959
                     /locus_tag="ACG89C_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4900259.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="ACG89C_001722"
     CDS             10034..11146
                     /locus_tag="ACG89C_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4900260.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="ACG89C_001723"
     CDS             complement(11218..12855)
                     /locus_tag="ACG89C_001723"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4900261.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="ACG89C_001724"
     CDS             complement(13023..13631)
                     /locus_tag="ACG89C_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4900262.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3689 a         2885 c         2452 g         4662 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcatt
//
LOCUS       DAZUKR010000067        13397 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000067 DAZUKR010000000
VERSION     DAZUKR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.068"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            284..2023
                     /gene="yfcC"
                     /locus_tag="ACG89C_001725"
     CDS             284..2023
                     /gene="yfcC"
                     /locus_tag="ACG89C_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4900263.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2180..3364
                     /gene="iadA"
                     /locus_tag="ACG89C_001726"
     CDS             2180..3364
                     /gene="iadA"
                     /locus_tag="ACG89C_001726"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4900264.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3475..3678
                     /locus_tag="ACG89C_001727"
     CDS             3475..3678
                     /locus_tag="ACG89C_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4900265.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3779..4441)
                     /locus_tag="ACG89C_001728"
     CDS             complement(3779..4441)
                     /locus_tag="ACG89C_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900266.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4454..5956)
                     /locus_tag="ACG89C_001729"
     CDS             complement(4454..5956)
                     /locus_tag="ACG89C_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4900267.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6504..8183
                     /locus_tag="ACG89C_001730"
     CDS             6504..8183
                     /locus_tag="ACG89C_001730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4900268.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8310..8789
                     /locus_tag="ACG89C_001731"
     CDS             8310..8789
                     /locus_tag="ACG89C_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS4900269.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9008..9688
                     /locus_tag="ACG89C_001732"
     CDS             9008..9688
                     /locus_tag="ACG89C_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4900270.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9696..11027
                     /locus_tag="ACG89C_001733"
     CDS             9696..11027
                     /locus_tag="ACG89C_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS4900271.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11042..12004
                     /locus_tag="ACG89C_001734"
     CDS             11042..12004
                     /locus_tag="ACG89C_001734"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS4900272.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12115..12453
                     /locus_tag="ACG89C_001735"
     CDS             12115..12453
                     /locus_tag="ACG89C_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4900273.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12532..13239
                     /locus_tag="ACG89C_001736"
     CDS             12532..13239
                     /locus_tag="ACG89C_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS4900274.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4298 a         2347 c         2924 g         3828 t
ORIGIN      
        1 atgaaattca atttttcaat gattgtaaaa tgaaaaaaac aggacgagat tttctctaat
       61 ctaaaaataa aaaaagaatt ttaaggaaaa acagagaaat aaaatactta atataaatgg
      121 aaagagaaaa aaataatcat acataggaga aaaaaaacaa aatagaaaaa agtgattttc
      181 taataaatta ttcatgtgga aatgtcattt ttactgtata attcatttga ttatccattt
      241 tttattcatc aaaattactg aaaaaagaaa ggaagaaata aatttgaaga aaacaaattc
      301 ttcaggaaaa ccgaaacgcg acttcactaa attatcgaca ccccatacgt atgtcattat
      361 ttttggtgta gtcatctttg catggatact gacttttgtc gttccagcag gaaagttcag
      421 cacgcaagat attgaataca aagatgcaaa tggcgaaaca agtactcgta ctgttttacg
      481 tcaagattcc ttccgttatg cttacgagct ggataagtcg tatgtgtttg atcaattaga
      541 agaactgcaa gatcatccag cagaacgtga aaaactggat gttccggaaa aaggattgga
      601 aaaagtcatt gctgacggag aaaaaaatct tacgcaggaa aaactagatg aaatctcctt
      661 gacagatgat gtgctttacg atcagtacgg cgaaaatatc tatgatactt ctaaaaaatt
      721 gcataaaaca gccaaaatat ggggaacaga tgattttggc ggctttggtt tcttaaattt
      781 tgtttttgaa ggactagtat ctggagataa gtatggatct gctgtaggga tcgctgcatt
      841 gatccttgtt gttggaggtg cttttggtat tattatgcgt accggagcga tcgacgcagg
      901 tatctatgcg tttatcagta aaactaaggg actggagcgt ttagctttac cgctattatt
      961 ctttgctttt tcattcggtg gcgctacatt cgggatggca gaagaggtaa ttccattctc
     1021 aatggtgatg gttccatttg tcattgcgtt aggatatgac tcgatcgtag cagtaaccgt
     1081 gacttatgtc gcatctcaag tcggaaatgc gacctcttgg atgagcccgt ttagcgtagc
     1141 ggtggctcaa gggattgctg gaatccccgt gttgtctggt gcgacattcc gattgattat
     1201 gtgggtagtt gttactgcat tagccgctgg ttatatgatg atatatgcag aaaaaatccg
     1261 taaaaaacca gaaagttcgt tgacttacaa atcagatgat tatttccgtt ctcatatcaa
     1321 aaagacatct gatgaaaata aaaaattcat gcttgggcat aaattgatct tattagagat
     1381 gttagttgtc cttgtctgga tcgtttgggg cgtgactcaa aaaggctatt acattccaga
     1441 aatcgcttct caattctttg tgatgggatt agttgctgga atcattgctg ttcttttcaa
     1501 attagatgga atgaaagtca atgacatcgc ttcttctttc caatctggtg cagctgattt
     1561 agctggtaca gcaatcgtag taggtatggc caaagggatt cttcttgtct tgggaggatc
     1621 tgatgcatct gttccttcag ctttgaatac gattttgcat gggatcggga cggcgttgac
     1681 cggtgttcca gctgtcattg gtgcttgggc gatgtacatc ttccaaagtt tgttcaactt
     1741 ggttgttaca tcaaattcag gacaagcagc tttgactatg ccaatcatgg ctccactggc
     1801 agatttagta ggtgtatcca gacaagtagc agttcttgct tatcagcttg gttcaggttt
     1861 tatggacgct tttacacccg tatcagccag cttgattggt gtgctaggtg ttgcacggat
     1921 cgaatgggca aaatgggcaa gattccaaat caagatgcaa ggattcctgt ttgttttagg
     1981 aactattttc atcatgattg ccattgcaat aggtctgcag tagtactgga gacctgtaaa
     2041 taaaaaagta atccaaacag tagataagga ctccaagaac cgtcatttta cggtaaaatc
     2101 aaaggagtaa agatcctatc gtttggatta ttttttaaga tcctatcatc agttatactt
     2161 aaagaaagag gaaaaatgta tgaaaatcat tcgacaaatt gaagtgtttg caccagatta
     2221 cttaggaaaa atggatgtac tgattgccgg tgggaagatt ttggctgtag aagaacagct
     2281 agaaggcggc tacgaaggag tggaagtaga agaactttca ggtgagggaa aagtactaac
     2341 cccgggtttc attgactgtc atttccatat tttaggtgga ggaggagaag gcggttatca
     2401 aaatcgaacg ccagaagtca cattgagcca attgactaca gctggtgtaa cgacagttgt
     2461 cggttgtttg ggaacagacg gagaaggtag agatatgact gcgttgatca gcaaagcgaa
     2521 aggtctggaa gcagaaggaa tctccactta tgtatacgaa ggttcgtacc gattgccggt
     2581 aaaaacagta acagattcta tcatcaaaga ctttttgaca attgataaaa ttatcggaat
     2641 cggagaaatc gcagtatccg atcaccgttc ttctcagcca agttttgaag agttcgctcg
     2701 tgcagtagca gatgctcgtg taggaggcat gttatccgga aaagctggga tcatcaatgt
     2761 ccacttaggc ggaggaaaaa gaaagctaga gctattgaca cgaatcgtag aagaaacaga
     2821 aattccgatc acacaatttt tgcctactca tgcaaatcgc acaccagaat tatttgaagc
     2881 ttgcgtggca tttgccaaac gtggtggcac gattgacttc actgctagcg aagatcctga
     2941 tttttgggag aagacagatg gtgaagttcg cttcagcaaa gcactgaaac gattgatcga
     3001 ggaagatgtt tctttagata atttcacgat gacctcagat ggacaaggaa gcttgcctta
     3061 ttttgatgaa aacaatcatt tcctagggtt aggagtagga agtgccaagg cattgctagt
     3121 agggatcaaa gaagctgtgc aaaaagaaag tatccctttg gaaatcgcct tacgagctat
     3181 aaccagtaat ccagcacgta tattgaaatt ggataaaaaa ggaaaaatcg aaattggtgc
     3241 tgatgcagat ctgtgtatct tggataaaga aacgcttgat attgatacag ttatcgctaa
     3301 aggagaaata atggtccaag aaaaagaagt caaagtatgg gggactttcg aaaaaagttt
     3361 ttaatcaaca gctctaacta tcttttttag aaaacgagcg ttctgtttgt ctgtatctgt
     3421 tgttgatgtt accctgaagg tagatcaata aacaaactaa gaggaggaag cacaatggca
     3481 gatttaaaag gacgttctaa agatatgaaa gataaagtag tcggtgaagg aaaagaagca
     3541 tacggcaaag caacagacga caagagcaaa gaaacagaag ggaaagcaca atcactaggt
     3601 gctgacatca aagaaaaagc tcgaaattta ggcgacgaag tcaaagacaa gttttctcga
     3661 aaagacgaaa aaaaataaaa accacttatt actaattgaa tcaagcaaat aaaaaatccg
     3721 aaccatatct gcgagacaga tatggttcgg attttttatt tgatcattat cctgtttttt
     3781 aaacttcgat tttttgaaat tttttatttt ttagttcaaa gacttcatca cagctttttg
     3841 ctacttcaat ctcatgagtc acgatgataa tacagcgatt ttgttcatgg gcgatttctt
     3901 ggaaaagttt gacgatatct tgtgaagttg tttcatctag atttccagta ggctcatccg
     3961 ctacgattag ttgatgttca cagcagatag cacgaacgat agcgactcgc tgctgctgac
     4021 ctcccgaaag tttggtcacc tgtttttttg ctaatatttc atcaattcct acacgagcca
     4081 gtcgatctaa agcaacttgt cgataattct cttgcttggt cttagtgatt tccattgctg
     4141 ctatgacgtt gtctagagcc gataaataag gaagcaagtt gtaagcttgg aaaataatcg
     4201 agacatcatt tttacgataa cttcttaacc cgattttgtt cagtgaacga tcttgatagt
     4261 atacctttcc ttcttttggt gcatctagtc cagaaataag cgaaagaaaa gtcgtttttc
     4321 cagaaccact tgctcctaaa atagcgtaca tttttccagc ttcgaaattc agttctacat
     4381 ctttataaag cgcatcttgc ggatcttgat accaaaatcc caattgttct gtccgtaaca
     4441 tcaaattcct cctttagttt aataggattt ttcttgggtt taatcttaat atccctatag
     4501 aagctaaaaa gacagaaaac agactgattc caaatgctac tccggctaat agtcccaatt
     4561 gtgcaggttt gacattgatt tccaattcgc tgacttgatc agaaacagca aatggattat
     4621 tcgtggttcc ttgttgacca tcgcgttgac cgccttgttc ttgtctgtta cctggcattt
     4681 gtccaccgcc aggagcttgc tggttttgtg tcgtggtttc tgtctgttgg tcaagtagct
     4741 gctgtcccac ggcattcgcg acgacgtttc cgctaagcga agcaactcct aatgcaagaa
     4801 tcatacaaat agccacttcg gtaaacaatt ggagaatgat tttacttctg gattctccta
     4861 aagatagcaa gacgcctaac tcatgccgac gctcccgaat ggtaatcata atgatcagag
     4921 taagtatgat aataccggct acagccacta gtaaaacaac atttttagaa aaacttgcca
     4981 cgttgtttaa aggttccaac attgactgat acatggaatc atttgattgt aagctaaatg
     5041 tatctgtgtc gatcagcttc tcagcttctt tgagaaactc atccatttta tctggatcag
     5101 atagagtgta aatagcagaa tctatcgtgt tatcgtcaga tgtcccattt agttcatttg
     5161 caaaagtgta agaagtgtaa agtgtattcg acggattcat gaagttgaat ttcataccca
     5221 tactgccgct tgtttcgctg gattcataaa ttcctttgat cgtcatttca taagtggtat
     5281 cttcatcttc agtactcgtg attgtgaaag tatctccgac tgccaggtca tttgtttctg
     5341 ctagagtaga atcaatgact acattgtttg tgcctttgtc gctgtcatct attccttcac
     5401 catccgtgat ttttgctgtt ccatcagaaa aattactatt ttgtgcagtt gctgacacac
     5461 cactgatttg aaagtctgct tgagtcattt gcggattccc gcccattcct ccagcaggtt
     5521 caccgcctgc atcagtagtt tctgaactgt cttcacttga atcagaacta gtgatggcag
     5581 ttattccatc ttttgccata gctgaggcgg aactttcaaa ggaataactc tttacattat
     5641 ctaattcagc tattttttcc gcgtccgtta aggaaactgg tgtaagagaa aaactgcctg
     5701 gatctggacg ttcttcagtg gtagaaccgc ttgaagaagt ttgttcctgt tttttaaatg
     5761 aagcttctcg attggcactt aaagtcactg ttgctccaac ttctttttgt gcttgattcg
     5821 ctgcttcttc tgcagcgctt cgaatcgtga ggccagctag cacaaaaata agaatggctg
     5881 tgaacacggc aaacaataac aatgaacgtc cccattttac ttttgtactt ttccatgcac
     5941 gtttcataaa attcatattt tgcatccctc ctagtagatg catttgataa tagacaacct
     6001 atcttaaagc tagcttaaat cttatgctgt ttgcacacca tcaacatgag gttctgtata
     6061 tataaaaagt cactatagtt attgcaaaaa atgtggtaaa atgaaaaaag tatgtttcaa
     6121 accgacgaag aagaaatgaa agaggaatag caaaagaaat cctagatgct acagagggat
     6181 ttttacgtgc atagttgttc taacaattgc tgatactagc gatttagttt atttgttgta
     6241 taataatggg gaacagctga atacttatat aagtattctg gctatagcgt ttcgcaaaag
     6301 atccattttg ttaatgaagt atatccaaaa taaaatgccc atccaactgt cagaacgcgg
     6361 atgtgctggt aattttcctg ctgattctcc ataagttgag aaatagtgat tttgcatatg
     6421 cttcattgga atggctcata tatttagata aagaagaaaa tcgacatctt taaacttgga
     6481 tgtcataaga agaggtgaag tttgtgagta ccattaaaat tatcccatta ggcggcgttc
     6541 gtgaaaatgg aaaaaacatg tatgtcgcag aagtgggcga ggatattttt attttagatt
     6601 gtgggctaaa atatcctgaa aatgagttgt tggggatcga cgtcgtaatc cctgatttta
     6661 cgtatttaga agaaaacatt gatcgtgtag ctggtgtctt tttaacacat ggacatgcag
     6721 atgcgatcgg ggcacttcct tacttgttgt caaaaatttc tgtaccagta ttcgggacag
     6781 agttgacgat cgagttagca aaattgaatg tcagtcgtaa tgaagcagcc aaaggattta
     6841 aggatttcca tgtggtagat gagcatacag aaatagactt tggtgaaaca gtagtgagct
     6901 ttttccgtac cactcatacg ataccagatt cagtggggat caatctgaaa acaaaagaag
     6961 gtagtatcgt ttatacggga gattttaaat ttgaccaaac ggctatccca atgtatcaaa
     7021 cagattatgc acgattggcg gaaatcggca aagaaggagt cttagcctta ctaagtgaat
     7081 cggcaaatgc agaaaatcct cagcctgtag cttctgaagc ccaaatagca gatgaagtat
     7141 ttgatacgat caaatattgg gaagggcgta tcatcgtcgc ttgtgtagca agtaatttgc
     7201 aacgagtgca acaagtattg gatgcagcag aaaaagcaca acgaaaagtt gtcttgactg
     7261 gtcaagattt cgaacgaatt attcgcacag cgatgcgttt agaaaaactt caattaccaa
     7321 gtgaagatct gcttgttacg ttgaaagaca tgaaaaacta tgatccagaa gaactgatca
     7381 ttttagaaac tggccgaatg ggtgaaccca tcaagtctct gcaaaaaatg gcaaacggta
     7441 cacaccgtaa tgtcaagatt gaagaaggag atttggttta tatcactact acgccaagca
     7501 tcgcgatgga aaccatcgtg gcgaaaacgg aagatatcat ttatcgagct ggtggaacag
     7561 tgaagttgat ctctgagaat atgcgcgttt ctggccatgc caatcctaat gacttgcaat
     7621 taatgatcaa tttgatgaaa ccaacatatt ttgtgccagt tcaaggagag tatcgtgaat
     7681 tagctgctca tgcagatttg gctcatgcag tagggatgcc atacaaaaat atctatatta
     7741 ctggacgcgg agacgtgctg gaatataaga acaagcgtat gagtgttgca ggaactacaa
     7801 gtgcagaaaa tgtaatgatt gatggattag gcataggtga tatcggaaac attgttttac
     7861 gtgatcgaaa aatcttgtca gaagacggga ttttcgtagc ggttgtcact atcagtcggc
     7921 gagaaaagaa aatcatttca aaacctcaaa ttacttcacg gggattcgtt tacgtaaaag
     7981 ctagccgtga tctgatgaaa gaaagcagca atatgattga aacaatcgtt gagaaacatt
     8041 tagaaagcaa tgattttgag tggagcaaat taaagcaaga catccgagat caattaggcc
     8101 gttacttatt tgaacaaaca aaacgccgcc cagtgatctt gccagtcatt atggaagcta
     8161 ctcaacgtcg aaataaaaaa taaacaaaaa gagtaagatg aacctctcta tagagtgctc
     8221 gtcttgctct ttttttgcga aaaacaaata atttttgaat acagcatccc tcgctttata
     8281 ataaacgata gaataaaggg agtgaatcaa tgatgagtca aacaaaagtc gaacgttatt
     8341 tagaagaaca aaagatcagc tatcaaccac ttgattttag tcatgtggta gaagaatctt
     8401 tcacagaaga actacaaaaa agagggattg atccagcttt gatatgtaaa actctcgtag
     8461 tgaaaggaaa caaaactggg gtgatcatcg cattgattcc tttgaacgat cacttagatt
     8521 acaaaaaaac gagacaagtg acgaaagatc gtaaagttgg ttttcctgga atggattttg
     8581 tattaatgca tacaggatat ccccacggag caaacacccc tattggaata aaactcgcac
     8641 atcctgatta ttgtttttta gcagacagtt ctttaaaaga aaaagaagaa gtaattgtgt
     8701 caagcggtga aatcgggaga agccttcgga taaaagtagc cgatttagaa aagatcgtcc
     8761 atcctattta cgctgattta attagttaag ggacgagaag agaagctaag cagagcagag
     8821 acctcaaact tgagattata tctgcttggt acttggtgca tcctgtatta tctgttacaa
     8881 ttgctttgta catactgtac tatcaatcca atgaaaatat atttagcagc tatatttttt
     8941 cattgatgtg caagtgatga aatcgttttt aataaaaaag atacagagaa aacgcgaggt
     9001 gtaaaatatg aaaattacgt atcatggaca ttcctgttta acaattgaaa tggaaaatgg
     9061 ccaacgcgtg atgattgacc cgtttattac aggaaatcca ttgacagact taactgcaga
     9121 agaagtaaag acagattggt tgctgattac tcatggtcat agtgaccaca taggagatat
     9181 gattcctatt gcaaaagaaa atcaagccac aattatcagt attgttgaga ttgctgatta
     9241 tgcgaacagc agaggagtag attcattcgg tatgaatatc ggtggaaaac atgcgtttcc
     9301 attcggcgtg gtaaaattcg tacatgcaca gcatagttca agctatgaag tagacggtat
     9361 cgttcagtat atgggcgagc ctagcggaat tattattcaa gcagaaggaa aaacgattta
     9421 tcatgcaggg gatactgctt atttcagtga tctaggattg cttgcagaag aattcgatat
     9481 tgatgtcgct ttcttgccga ttggcgataa ctatacgatg gggccagaag atgcagcaaa
     9541 ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc cattacaata ctttccctgt
     9601 cattgaacaa gatcccgaat cttttgtaga gttactggaa gatagtgaag gaaaaataat
     9661 gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc gacaaaacat gatttgattt
     9721 tagaatatat tgaaagttta cctgttggca atcgaatttc tgtaagaagc atcgcaaaag
     9781 aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa agatgcagaa aatgtcggac
     9841 tagtttcaac aattcaacga gtagggacca tccgtatcga acgcaaatta aaaaaacata
     9901 ttgaacgttt gacatttggc gaggtcgtca ggatcattga aggggacgtt ttaggcgggg
     9961 cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg cgcgatgact aaacatgcca
    10021 tggaacgcta tattacgcca ggatcactaa tgatcgtcgg caaccgggcg gaagtccaaa
    10081 aattagcatt ggaagatggc gccgcagtat tgatcacagg aggattcaat acgactgctg
    10141 agattgcaaa gctagctgat gatcttgaaa tgccgatctt acgcacctct tatgatacat
    10201 ttacagtcgc aaccatgatc aaccgagcgt taagcgacca attgatcaaa aaagatatca
    10261 tgcttgtcag tgatatctat atgccagctg aaaagactca ttatttgcag cagacagata
    10321 cgatttctga ttatcagcgt ttgtcagaag aaacgcagca ttcgcgtttt ccagtcgtca
    10381 accgccatca tcgtttgatg gggattgtta cggcaaaaga tgttttaggg aaatcgccaa
    10441 accagatcat cgatcgggtg atgacgaaag aaccaatcag tgtaaagaaa acaatgagta
    10501 ttgcctctgt tagccatcaa atgatctggg atgggctaga agtcatgccg gtcgtttccg
    10561 atgacttgac attggaaggc ttgattaccc ggcaagatgt aatgaaagcg atgcagcttg
    10621 tccagcgaca accgcagatt gcagatacga tttcggatca gatatctgga aatatccata
    10681 cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc taagttcaaa ttcgtagtag
    10741 caccacaaat ggtcaatggc gttggaacca tttcttttgg cgtacttagc gaaatcattt
    10801 ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg aaatatcttg attgaacaag
    10861 tgaacctaca ttatttgcgg ctgatccaat tggaaagcga attagacatt cgtccacgtg
    10921 tattggaaat cggcagacgt tccgctaaac tggatatcga agtgtttatt gaaaacacca
    10981 tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg ttcttagaaa gcaggagtga
    11041 aatgacagta cagcaagaaa tagtagatac aataaaaaaa tacgataaaa tcattatcca
    11101 caggcatttg cgccctgatc ctgatgcatt aggatcacaa gtaggattag ccgaactatt
    11161 acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt gaaacgattg aagggcttcg
    11221 ctttttagca gaaatgcaag aagttgaaga tgtcttctac caaggagcgc tagtcatcgt
    11281 gactgataca gctaattcac ccagaatcag tgatcaaaga tatcagttgg cagaaaaact
    11341 gatcaaaatc gaccatcatc ccaatgatga cccttatgga gatcttgtgt gggtaaatac
    11401 aaaagcaagt agctgtagtg agatgattgc agaatttgct ttgatgtttc ctgaatcttt
    11461 aaagatgaac gttaaggctg cacggttact ttatgcagga atcgtcggag atacagggcg
    11521 ctttttgtat ccctccacga cgtcccgtac attagagatt gcttcgatat tgagaaatta
    11581 tccatttgat gcaagtgctt tgaacagaga gattgaacaa atgccgatga aagtcgctaa
    11641 actttccggt tatctttatc aaaatatcca ggtggatgaa aatggtgcag gaagaataat
    11701 ccttccgcaa gaaattcttg ataaatatgg aatcgatgat tcagagacag cttcgatcgt
    11761 ttcacttcct ggagtgattg acgaagtgct ggcttggggg atttttgttc agcagccttc
    11821 agggtattac cgcgtacgtt tacgttctaa ggggccgatc atcaatgaat tggctaaacg
    11881 ccatcatgga ggaggccatc cattagcaag tggtgcgaat gcaaaagatt tagcagaagt
    11941 agaggaaatc tataaagaga tccaagatat atgccaatcg tataagagca gaaaaacaga
    12001 atgatacttc gttcgaaaac gtgccttttg tagtaaaaat cacaaatgta tcttttcaag
    12061 atcttctttt tccgttacaa tagtgctcac taaagatttt aaaggagaaa gaagatgaca
    12121 ttaccaaatt gcccagaatg tggctcagag tacacatatg aagatcgagg cttgttgatt
    12181 tgcccagaat gcgggaacga atggaacaaa gaagaacaag aaactgtcat agaagacgga
    12241 ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg gcgatagcgt gacagtgatt
    12301 aaagacttga aagtaaaagg tgcgtcaggt gccattaaac aaggaacaaa agtcaagaac
    12361 atccgtttag tagaaggcga tcataatatc gattgcaagg tagaaggatt tggaccgatg
    12421 aaactgaaat cggaatttgt gaaaaagaat taaagaatat tcaaggtctt ttcacattct
    12481 tttcctacta ctaatgataa aataaagtaa acgaaaagga gtgaaacgaa aatgtatggt
    12541 tatggaatgt atggtttttt agatccgaca tttcttttgg tcattcttgg attagttatt
    12601 tccggaattg cgtcagcgta cgtcaatagt actttccgaa aatacgatca agtgagaagc
    12661 aaaaacaatg tgactggtac acaagcagca caatatatat tacaaagtca acagataaat
    12721 aatgtcggtg ttcaacaaat tgccggagat ctcacagata actacaattc aggtaataag
    12781 atgttgagtt tgtctcaagc aactgcacag tctacttcag ttgctgcgat aggtgtagca
    12841 gctcatgaat gcggacatgc ggtacaagat gcttcgggtt atgtcccatt gaagattcgt
    12901 gcagcacttg ttccagcagc taactttggt tcgatgattt cgttcccctt gattatggta
    12961 ggtgtattgc ttagctggaa ctctacgtta atcaatatcg ggattttcgc tttttcttta
    13021 gcattacttt tccaattggt gacattacca gtcgaattta acgcgtctcg gcgagcaatc
    13081 caaattttga gtgaaggcgg actactgacg gaagaagaag taccgatggc aagacacgta
    13141 ttatttgcag cagcactgac ttatgttgca gcagcgcttt cgacattcct gcagctgttg
    13201 cgcttgatct tattgttcgg cggcaaccga agagattaaa ataaagaagt tgtgaaatca
    13261 gcgctttgat ctgagctaat accgagagtt agctgattga ataccattta tttggaataa
    13321 gggctctttg tcaataagga ctgatgggtt gtacaaaatc aaatctgggt cagaatgaac
    13381 ctcattctgg ctcagat
//
LOCUS       DAZUKR010000068        13348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000068 DAZUKR010000000
VERSION     DAZUKR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.069"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..166
                     /locus_tag="ACG89C_001737"
     CDS             <1..166
                     /locus_tag="ACG89C_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS4900275.1"
                     /translation="KKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENR
                     KKAMRKRVSR"
     gene            complement(228..2039)
                     /locus_tag="ACG89C_001738"
     CDS             complement(228..2039)
                     /locus_tag="ACG89C_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS4900276.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2036..2401)
                     /locus_tag="ACG89C_001739"
     CDS             complement(2036..2401)
                     /locus_tag="ACG89C_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4900277.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2564..2959)
                     /locus_tag="ACG89C_001740"
     CDS             complement(2564..2959)
                     /locus_tag="ACG89C_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4900278.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2977..3939)
                     /locus_tag="ACG89C_001741"
     CDS             complement(2977..3939)
                     /locus_tag="ACG89C_001741"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS4900279.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3939..4151)
                     /locus_tag="ACG89C_001742"
     CDS             complement(3939..4151)
                     /locus_tag="ACG89C_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS4900280.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4172..4870)
                     /locus_tag="ACG89C_001743"
     CDS             complement(4172..4870)
                     /locus_tag="ACG89C_001743"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4900281.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4890..5432)
                     /locus_tag="ACG89C_001744"
     CDS             complement(4890..5432)
                     /locus_tag="ACG89C_001744"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4900282.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5564..6568)
                     /locus_tag="ACG89C_001745"
     CDS             complement(5564..6568)
                     /locus_tag="ACG89C_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4900283.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6565..7554)
                     /locus_tag="ACG89C_001746"
     CDS             complement(6565..7554)
                     /locus_tag="ACG89C_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4900284.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7551..8357)
                     /locus_tag="ACG89C_001747"
     CDS             complement(7551..8357)
                     /locus_tag="ACG89C_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900285.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8523..9479
                     /locus_tag="ACG89C_001748"
     CDS             8523..9479
                     /locus_tag="ACG89C_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4900286.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9556..10074)
                     /locus_tag="ACG89C_001749"
     CDS             complement(9556..10074)
                     /locus_tag="ACG89C_001749"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4900287.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10162..10311)
                     /gene="rpmG"
                     /locus_tag="ACG89C_001750"
     CDS             complement(10162..10311)
                     /gene="rpmG"
                     /locus_tag="ACG89C_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4900288.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10539..10985
                     /locus_tag="ACG89C_001751"
     CDS             10539..10985
                     /locus_tag="ACG89C_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4900289.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11180..13075)
                     /gene="asnB"
                     /locus_tag="ACG89C_001752"
     CDS             complement(11180..13075)
                     /gene="asnB"
                     /locus_tag="ACG89C_001752"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS4900290.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4156 a         2831 c         2277 g         4084 t
ORIGIN      
        1 gaaaaaattt gtatctggaa tattagtagg aagtctggca acagccgctg ccgttgctgg
       61 tttggttgct tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag aagcaatgat
      121 cgaagaaaac cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat tatttaaaaa
      181 tacgcatgaa gactttctaa aaaacagata agtcatcatg cgtattttta tttgtcagtc
      241 aatataagtt gtgctatttt atctgcctgt ctcagattcc atggatctgt ataaggtcta
      301 gctactgctg cagttagcca atccacacct tcaacaggta ttccatagca ataaaagtca
      361 gactggattt cccctgaagc atcaatcacg cgattcgtac ctcgctcaac atctatagct
      421 tctgaatgat aacttcctgt attatctgaa aatgaaaaag ggcgaacaag tttgttttcc
      481 ttcaaatttt ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg caatcgagct
      541 tcaatcaaat agcgactgct gatttcttta tcttgtagag aaataacata gcggttgtgc
      601 ttttgctgaa tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct
      661 gctaattctt tcgtccgaat gattggcgga ccgatcgtta aaaagctatt tagatgagta
      721 aaccatcccc atagtatttc tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc
      781 tgccggattg gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg
      841 caattgcctt tttccgcttc ttttatctgc ccttcaataa atattcggct agcttctgaa
      901 aagggctcat tctgataggc aagaggagtt tctaaatacg accaatccca tctataaggg
      961 attagtgctg gatgctttcc gagacactcc tctgaagaga gggagagaaa atcatgctca
     1021 aaatttttct ttggaatcgg caagtgatat tcttcgatga ttttcttgta ataaaacaac
     1081 tcaacgtctt ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc
     1141 cattgctcca ggttttcttt tgttagtaac cgtggccatg ccattgctcc gccttgcttt
     1201 tgatttcttc ctcgagggta ataagggagc ccccttcttg atccactgta tacaatcggt
     1261 tccttgcctg atggatgata gaccagtttt cccttttcgt tctcgaagcg ccctcctctt
     1321 gatactgtaa acaatccgac ataatcaaaa aaacttaagc ctagacctct taaaaagaca
     1381 ttttctttag gcggtatctt cgaaagatct acatctgcag gattagaagg aagctgatac
     1441 agtaaaacat ttttctttgc gtagtctacc cacactcttt cttcttctgt ctcatcattt
     1501 tcccagtgac cgcttgctaa aacaagctga tcgatagaat agctttgctg atctgtctgt
     1561 aaaagataac catcttttct tttgcttact ttttttacta aagaataaac aaactgaata
     1621 gaagctggaa attcttcttg caactttgag aaaaaccagc gttgatagag tccgtataaa
     1681 catcgagtag attgctgatc tttttccaga cgattgattt cttctataat tagttgctta
     1741 tttttggtta ccgtctgttc gatataatct gtagcaggtc ctttactcca ttgaaacaag
     1801 ttgggtccct gtgttaccaa tcctccactg gacaatgttt catctgtaaa aagcgtcact
     1861 tgttgagaaa cagaattcat taataactcc ggtggctgat tcaaccgcca aactcgacct
     1921 ccaggaccat tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg attattatgg
     1981 agaatccttt ctgctacact taatcctcta ggtccggcac caatgatccc tattttcatt
     2041 tgcatgcctc catttcgata aattttttca ataaaacagc atggttctga tgttcatttt
     2101 ttgcaccata aacaaaagta atgactggat gttctttcat aagttgactg agtttaaaaa
     2161 tagcttgctt cttttcttct tcgaaaatca attcttgctg gtatttcttt gcaaatgaag
     2221 caaatcgttc tggtacatgg tcaaaagttt tgcgtgcttc attactaggt gcaatttctt
     2281 tcaaccacag atctaactgc gctttttctt ttgaaattcc tcgtggccag aatctatcca
     2341 cgagtattct atagccatcc tctttttcag catcaaggta aacacgtttt aatttgatca
     2401 tagttgtttc cttctttcta ccttttcgct tagcttatat atgtagataa tagcacaaaa
     2461 taaaaaaaat gaacggcaca actatccatt gcgccgttca tgtgatcttg gtcaaaatct
     2521 aatgattgat tcggttttta aagctgtcat acaatcatct gttttatact tttttacttt
     2581 tgatttttcc agaccatccg tcatatccac ccttaagtaa ataaatctct tgataacctg
     2641 ctttacgtaa tttatttgct gtacggatgc tcaaactttt cttttgatcg taaatataga
     2701 caggctggtc ttttcgaata gatcctaatg aatctttcaa aacagtgtaa gggatgtttc
     2761 ttgcacctaa aatatgccca gcatcaaaca catctttttc gcgaacatcg attacttgtg
     2821 cttttctcat tccttctcga aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca
     2881 ttactcgtag atagacttcc caaaggatga ttgcaagcac aatcaatagc aaaatgatat
     2941 taattaccca taaaaccatg taatgcctcc gtttttttaa gcaaatttca aggctaatga
     3001 ggcagcacca atgacaccag cttcattacc aagctctgct aatttgattt ttgttgattc
     3061 tcttacttgc gggaaagtaa attctttaaa atatttctct acacggctac gtaaaaattc
     3121 tcctgctgcc gatactcctc cacctaacac gatacttgac ggatttaatg tattccccaa
     3181 gtttccgcaa gctaaaccta agtagaagca tacgcgatca acaaccatca atgcaaacgg
     3241 atcgtcttct tgagccattt caaagatatc tttacttgtt acttcttctc cgttgtctag
     3301 cattgctttc agttttgaat cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac
     3361 cacacctgta gcgctagata cagtttctaa acatccgcgt ttaccgcaag tacattcgaa
     3421 cccttcagga tccacagtga tatgtccgat ttctcctcca gctccgctca cgccgtgaat
     3481 caaatttccg ccagcaacga tacctccgcc aacacctgtt cctagagtaa cgaatacaac
     3541 gtctggatcg ttttctccag cacctttcca tcgttcacct aaagcagcaa cattcgcgtc
     3601 attatcgata gcgaatttga ttcctgttcc tgcctcgatc agttttttca caaattgaac
     3661 ttctgtccag tttaagttat acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc
     3721 tggggtaccc atgccgattc cgataaagtc ttcggctttc atattatata aattcaaacg
     3781 atgattgatt gattcaatga tttcaggaac gatatgctta ccatcatcca aaatattggt
     3841 atcgatgctc catttttgct gaatctctcc ttctggtgtt aaaattgcaa attttgctgt
     3901 tgttccacct aaatcaatcc caataatctt cttgtccatt acaaatttcc tttctttcgg
     3961 ctttcttcaa ttcggtgctc tctttttaat atgcgccacg ctgtcatgta tgtatcatga
     4021 tcaatcaaac ggctatcata cagattacgc aattcgatag tcattaattc aatgtcatag
     4081 atgcgtgcac ccacatagat aaagatgcca aattttttga acagctgctg tacatcatat
     4141 aagctctcca tctttaagca cttccttcat attatacaag taaaccatat ttttttaaac
     4201 ccagtaaaat acatattaca aaaagaaata caaaaactag tgtagaaaga atcctttggt
     4261 gtatcgaata gcttgtctgt tgtttcggta gacccaaaac attccctaac actaatccgc
     4321 caattagccc accgacatgt ccccaaacat ctacagaggt atccagaaga ccaaaaacaa
     4381 atgtcaacac aataaataaa agaaaatgtc tgactagctg ctggatcgct gggttatgtt
     4441 taaaatgaaa ccctaagata aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac
     4501 caccagacag aacattcgct tcgttaaaag caaagcttgc tgtatttccc attatcccgc
     4561 tgaacatata aatcaagaaa aaacgccaat ggccatagat tgcctcgatt tgctgcccca
     4621 taaaatacag tatcagtgag ttcattacga aatgcatcag tccaaaatgg atgaagattg
     4681 gtgtcactaa acgccaccat tcattgaagt gaacaagata aggaccatac attccggcat
     4741 aataagggat ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct gtctgtatcc
     4801 ctaaaaaagc ataggtccat aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt
     4861 gataattcat gaaaacctcc catttcattt tagtcaataa acaattgttg gatcttctga
     4921 tcaaagctct ccggttgcca ttgttcattc aattgttcac gaaaaacaag gctacatgtt
     4981 tttttatgaa aattatttaa ataccggtcg taataaccac cgccaaaacc gattcgataa
     5041 ccggctctat tgtagataat acctggtaca atgaggagat cgatcatctc cggcaaatag
     5101 agcttctctg attgtggctc aagaacacca aaactcgaac gtacaagaac ggactgttca
     5161 tccatttcat aaaacgccat ctgacgtttc ggcaaagttt taggtacaac tattttcttt
     5221 tgttcttttt ctgctcgttg gataaccggc tgtgtatcca gttcaaattc attcgataaa
     5281 gtcgtcccta tcacttttgc tgacttccag tactcgctgg cgaatagctg tttatagatt
     5341 cgctgttctt tttccttttt cttgccactt ttggccagat ttttcagctt ttcgattcct
     5401 ttccggcgta atcttacttt caattggtcc aaaaactcac ccttccttct ctcataaaat
     5461 ggtataggaa aaaaagaaaa atagcaatcg aactttcaaa taaatgcata aatagaataa
     5521 aaagaagacc tagaaatgat tggccttcct tttttaatga atttcaactg ttcagcgcgt
     5581 gtttttgtct gataaaaagt gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa
     5641 ttcctgtagg tacttcccca cttggtagaa tcactctagc aaccatatct gcactaagca
     5701 gaaaaatgga accgaacaaa ccgctcagaa caaaggtcca gttattttct ttacgtacga
     5761 ctagcttcgc agcatgagga gctagtagtc ctacaaaaga taaactacct gtcaccgcta
     5821 cactaccgct tgccaaagca acagctaaca acaaaaggat tttttgactt ttagccacgt
     5881 taaggcctaa tccttgcgcg cgttcttgac caaatgatag caatacaatc ctctgtgcat
     5941 aaagtcctcc tagcggaatt aagataaccg cccatggaag caatgcccaa acataaggcc
     6001 agctcgcacc ccagattgat cccgctaacc atgcattcac aaatccataa ttttccttag
     6061 ataattggat cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg gcaataccgg
     6121 acaataagat tcgattcata cccaaaaagc catttcgatc ataggccgca agatagacac
     6181 agcaagcagc taaaacacca cctagaaagg ctacaactag caatagaaac ggcagcgaat
     6241 agtgcgaaaa gaaacctaaa taaagcatca ctaaaaatcc tgctccggca ttaatgccca
     6301 atatactaga atctgccaat tcatttcttg ttactccttg caagatataa ccagaaacgc
     6361 caagacaagc tcctgctaat aaactgatca gtaatcgagg aactcgaaat tctctaacaa
     6421 tcagcgccaa agaagatgat tctttcccta tgaacgtggc aaaaagctga tccaggtaaa
     6481 taaaaggcgt accaatcatt aaactaccta tagacaggat gacaagtaca aaaagcaaga
     6541 aatagaacgt ccaatatatt tttttcaccg ttgttccctc cttacttgta tcagtaaaaa
     6601 cggaacgcca atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat
     6661 cctcgctgta aaatccgcca acataaccaa tccaccgcca actaaaaaag agacaggtag
     6721 aacataacga tagtccgttc caaccattcc gcgaacaact ggcggaacca acagcccaat
     6781 aaatgaaaca ggtccgacta acgaaacggc aatggctgct aacactgcaa ctagacagaa
     6841 aacaatcaac cgcgttcgtt taggatgact tccaagagct aatgcatgtg tttcactcaa
     6901 atgaatcaaa gatattgctt ttcctaatga aaaagaaacc aaaacagcaa gtatatataa
     6961 aggaaacaat actgctaatt gtttccatgt gatattggca gctccgccta caaaccaaaa
     7021 agcaagatcc tgctgcagat taaagaattg ggtaagtgct tggctgatag cagtaaaaaa
     7081 agaactaata gctacgccag ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa
     7141 ctgcaaaaaa ccaaatacga gcaaagttac agcaaaagaa cctgccaatg acattagaaa
     7201 cacagaaaaa tcagaagctt taggccaaaa agcaaaacta agggccattg ccaacgaagc
     7261 acccgcgttg attcctagta agcctgaatc tgctaaagga tttcttgtca ccccttgcat
     7321 caatgcaccg ctcaatgcaa aacaactccc tacaagaaat gctcccgcca cacgaggcaa
     7381 acgaatattt cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg
     7441 ccaaaaatct gctttttcca cagtcgctgc gccatttgaa agagcaacaa aaaaaataat
     7501 caaaagaaca aaacagacca aaagcaggag tctatttttc ttcatagtcc tcataattga
     7561 tcctcctgct cttttggcaa caaatcatag gttagaaaaa ctggtttatt tgttcttgga
     7621 agcgtgacaa tcgttgcatc aatctcaaat atttttttca tacactcttt tgtgatcact
     7681 tcttcaggtg taccttctgt gatcaactta ccgtttttca ttgctaataa ataatctgaa
     7741 aaacgtgaag catgattaat gtcatgaatc gacatcaaga tcgttgtttg tttcatttga
     7801 ttgatttcag tcaataactg taggatctct aattggtgtg ccggatctaa aaatgttgtc
     7861 ggttcatcca agatcaagat atctgtttcc tgagcaagcg ccatagcgat ccagacacgc
     7921 tgggtttgtc caccagacag cgttgcgata tcccgcatac gaaatccagc tagacctgta
     7981 gcatccaacg cccactcaat tgcttcatga tcttgcgtgt ccatgccact gaacagtttt
     8041 tgataagggt atcgtccgta agatactact tcttctactg tcatacctgt agggtgttca
     8101 ctagtttgtg ctaataatgc aatttttttt gcaagttctt ttgaactgta tgtagagata
     8161 tcagagttat tgacaatgat ctttcctgat ttcacaggta gtatacgggt aaaacttttc
     8221 aataaagtag attttccact tccatttggc ccaatcaaac tagtgatttt cccttcagga
     8281 atcgtaacag acatgttttc aatgattttt ttgttttcgt agccggctgc aatgtcagct
     8341 gctcttaatc tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa ttatgacgct
     8401 gtttcaacat ttcgtcaagc gctactttca tatctatagc caaatgaagc ggttgacagc
     8461 gttcgaaata tcaggaataa taaccaatga gaaagattct caattatgtt aggaggaaag
     8521 aaatgaaatt aaaaacaaaa gtattcacat tatctagcat attgctgctg gctggagtac
     8581 tgagcgcttg ctccccctct tcagataaag aaacagctac aactgccaat tcgacgactt
     8641 catccaatag tactcataca gtcacagata cacttggaca taaagtagag attccaaacg
     8701 cgcccaaacg gatcatcggc agttacttag aagactatct gattgcacta ggggaaaaac
     8761 ctatcgccca gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa
     8821 aggatgtccc aacgatttct tatgaccttc cttatgagaa agtgttaagc tttgagcctg
     8881 atttgctgtt gatttcttct tccgctactg tcgaaggcgg gaaatacgaa caatatagca
     8941 aaatcgcgcc aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa
     9001 aagatgtcgg caaagctttg gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc
     9061 aaaaatccat caagaaaact cgagaagaac tagcagataa aatcgagaac aagacagctg
     9121 cagtgttgtg ggtgaccaac aactcagcct ttatggtcag caaaaatcgg tcaagtggaa
     9181 gaatcgtata cgacgatctg gaatttggtg ttcctgatct agtggaagag gtctccaaaa
     9241 aagccacttc tgactggtct gctgtttcct ctgaaaagct agctgaatta gatgctgact
     9301 acatcatcct tgtgaacagt gacaaagatg ctgccatgtt caatgaacca agctggaaga
     9361 atctaaaagc agtgacagac aatcatgtac tgcaattcgg acctgaatct agttggctat
     9421 ataacggacc tattgccagt caggaaatgg tcaatgatat caaagaaaaa ctaaattgat
     9481 tttaactaaa aaaagcatct tcacgattag tcgtgagatg ctttttttct tattaacaaa
     9541 ctagacgcat agattttatt cttcttttga agcaacccta ttttttactc tttcccattt
     9601 ttgaaaacga tgggcatata gattttcttg atccgttatc ccctcatact cctctataag
     9661 ggcgaagtct gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg tgctatcgat
     9721 caatgttcgc cagatccgac cagtgtaagg aagaagttct tgaaaaatcc gactaccacc
     9781 cgaaacatag atatcttctg agatcgtttg actatacgac aaaagttcat cgatcgaatg
     9841 cattacttcc gcattttctt tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg
     9901 gcgataagga agtgaaagat cccccattcc ttcataggtc tttcttccca tcactaaaat
     9961 tcgatccatt gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc atggtaacaa
    10021 accatttttg ccgatcagac catttttatc ttgtgcccac atggaaataa acatattatc
    10081 cgctccttat gattttagat gttttgagat aaaaaaagtc tcaattcttt gaccagcaag
    10141 gaattgagaa ctttaaaaac attattttgt ttcacggtgc aaagtaactt tgcgttcgcg
    10201 tgggcaatat ttttgttttt ccaaacgatc aggattgtta cgtttgtttt tgcttgttag
    10261 atagttacgt tctttacaag aagtacactc taaagtgatg tttacgcgca tgtttttccc
    10321 tcccattttt acaagaatcc tctaagatga ctctcagact tgattatctt accatgattt
    10381 tttctagatt gctagtgttt ttcacgattt tgttaagtaa aactacttga cgataaatac
    10441 aacggtttta cttctcaaac aaaatctttt tctttttgta taaataccct tacacttgtt
    10501 tatatagatt atgtttttta ataaaggggg atactaatat gccatggaac atgaaggact
    10561 ttcctgcatc aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa
    10621 atgcattatt agatgaaggg taccctgata accgtgcgat cccaattgcc attgatcaag
    10681 caaaaaaatg gtatgaagat gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa
    10741 atccaagcaa gacggatgaa cacgatacga atcctcgtgc tggaaaactg cttgattcag
    10801 atgtgatcgt agaatatgag gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg
    10861 caagcaacca ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa
    10921 acaaagaatc aactttgttc atttataaaa aagatgggac aaaagaaaaa gaaatcgatt
    10981 attgaacata tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa
    11041 caatggtgtt cctctgccaa ctccgaacca taagtcataa tatctataaa acgggaagag
    11101 ctgttttttg aaattgtttc ttatcactca gtgttaaacg gtaggagaaa acctcttttt
    11161 tattgatatt tgattttcat caaacagtca catagtcgat tgcttctgct tgcggaatcg
    11221 tttcatctac aaagtagact ttataccatg tcaagaaact gaagattgtc cagatttttc
    11281 tttgctcttc tttacgtcct tcgtgatggt catctagcaa cttcatgatt ttttcttgat
    11341 caaaaaattc ttttgcaaaa tctgcttcga ataatgcttt gacttgttcg tagccgtgtt
    11401 cttctttcaa ccaagctttg attggcacag ggaatcctaa tttcacacga ttcgaccatt
    11461 cttccggaag atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca
    11521 ataaaaactt acttggtatt tgttgagcca atttcatgac ttcgatatcc aataaaggta
    11581 ctcggacttc caaagaactt gccatagaaa gtttatctgc ctttaacaag atatcttttg
    11641 gcatccattg atgaagatcg acatattgca tcttattgac ttcatctttg attccttctg
    11701 ttttctcgta atgaacagtc atgatttcat ccacagatgg tgctgtttga tattttggtg
    11761 ttaacacttc tgatgcttct gattcttcga acactcgagc atgaccgata aaatattctt
    11821 ttgcaggagc aagtgattca tataaatgga tccgtccatg gaagtttttc atttttccga
    11881 ttttacgtcc taaattatag cgtgttcctt ttggtaattt tttcaatcct tgagcaatca
    11941 cacggatgaa ttttgaattc gtatggaatc catacgtttg gtatcccgca aacagttcat
    12001 ccgccccttc tccagattgg actacccgca cactttgtga agcaagattt gctaaaaagt
    12061 acaaaggtac gcaagaagga tttgaatcag gttcatctaa gtgatactgg atcaaaggga
    12121 atgctttgaa cgacatgtct ccgtcgatca ctgctgctgt attatctaag tccaatagtt
    12181 cggttagttt tctggcttca atcgcttcat tataggtttt atcatcaaac ccgattgaga
    12241 aagaatgatc tggtttcaat acagaggtta cataactgga atcaaccccg ctggataaga
    12301 aagaaccaac ttctacatca ctgaccgtat gtgcttcaat agaagcttga acggtttcgt
    12361 cgattttttc gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc
    12421 aatattgctg aatgtctatt tttccatctt tatacgtata ataatggcct tcaggcaaac
    12481 gatagacatc tttgaagaat gtttcgccgt ttaatggaga atattggaaa gtcatataag
    12541 gtttcaacgc ttctttgttc aattctttgt taaaatcagg atgaggcaga aaacttttga
    12601 tttctgaacc aaacataaat gttccgttca tttctgcata ataatatggt ttgatcccaa
    12661 agtggtctct tgcaccgaat aactcattct tttcattatc ccaaatagca aaagcaaaca
    12721 ttccgcggat cttttgcaat aattctgttc cccattcttc gtaaccatgc aaaaggactt
    12781 ctgtatctgc atgtgtttga aaaacgtggc cagctgcaat caattcttct ctcaaaggct
    12841 ggtaattata gatttcgcca ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag
    12901 gctgagtacc gccctctaag tcaataatac ttaatcttct gaatcctaaa gcaacatgtt
    12961 tatcgatata ttctcctgaa ctattaggtc cacggtgaac gattcgatcc atcattgtat
    13021 taataattgt ttttttgttg tccttgtcgt taacaaaacc tacaataccg cacatttaac
    13081 tttcacatcc taatataaaa ttactgttta tttcattcat atgcatctta tcatagaata
    13141 aatagcggac gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt
    13201 ttctgttttt acagaaaata aagtcccaat atctaattta ccatgttttc ttttttgagg
    13261 ctaccttatt ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa aaaaaccagc
    13321 aaaaaaagac acgacctaaa gatcgtgt
//
LOCUS       DAZUKR010000069        13176 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000069 DAZUKR010000000
VERSION     DAZUKR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.070"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..1262
                     /locus_tag="ACG89C_001753"
                     /pseudo
     CDS             121..1262
                     /locus_tag="ACG89C_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1388..2467)
                     /locus_tag="ACG89C_001754"
     CDS             complement(1388..2467)
                     /locus_tag="ACG89C_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4900291.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2570..3010)
                     /locus_tag="ACG89C_001755"
     CDS             complement(2570..3010)
                     /locus_tag="ACG89C_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4900292.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3067..3477)
                     /locus_tag="ACG89C_001756"
     CDS             complement(3067..3477)
                     /locus_tag="ACG89C_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4900293.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3657..4091)
                     /locus_tag="ACG89C_001757"
     CDS             complement(3657..4091)
                     /locus_tag="ACG89C_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4900294.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4191..5039)
                     /locus_tag="ACG89C_001758"
     CDS             complement(4191..5039)
                     /locus_tag="ACG89C_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4900295.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5428..7194
                     /locus_tag="ACG89C_001759"
     CDS             5428..7194
                     /locus_tag="ACG89C_001759"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS4900296.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7296..8555
                     /locus_tag="ACG89C_001760"
     CDS             7296..8555
                     /locus_tag="ACG89C_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4900297.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8702..10003
                     /locus_tag="ACG89C_001761"
     CDS             8702..10003
                     /locus_tag="ACG89C_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4900298.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10006..10857
                     /locus_tag="ACG89C_001762"
     CDS             10006..10857
                     /locus_tag="ACG89C_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4900299.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10870..11685
                     /locus_tag="ACG89C_001763"
     CDS             10870..11685
                     /locus_tag="ACG89C_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS4900300.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11836..13002
                     /locus_tag="ACG89C_001764"
     CDS             11836..13002
                     /locus_tag="ACG89C_001764"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4900301.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         4503 a         2363 c         2446 g         3864 t
ORIGIN      
        1 ttaaaacttg aaaatattac tgtttttttc ataaatttag acaaaaaaac agtaaacata
       61 taggtagaat ctctactatc ttatttcttg catacaaaaa aatcaaggag tgagagagtt
      121 atgagatatt caaccatctt aatatcaaca tttgcagcct taagcttatc gcttactata
      181 aatcagacag tccacacaga ggagacagaa acagccaaat ggacagaaac caagtgggcc
      241 aacgtcaatc gaattacctt tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa
      301 ttaagaaaag gacaaacagc gaccatcaaa gcaaaaattg actattcggg agacttagca
      361 cctatactac aatcagctgt tacatttgag aatgttgatc ccactttgtc gattggctat
      421 gacgagaaca ctttgactat ccaacacaag caagcgatat ttaccttcac cgttacatta
      481 aatcaggact tgttaaaacc agcgcttttt actgtcaagg tatcagatgg actaccgaac
      541 gatcatcatt taacgccgta tagtcagcgg cagacaattg agcaagattc agccatcgac
      601 tcaggaggag atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa
      661 gtaccgccta cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa
      721 cccgatacag aaactcaacc agagccagac aataaatcaa aacctgaaat aacccctgga
      781 gaaaaaccag atgtaactcc agaaccagat acggattctg gaaaccaaac agtaccggaa
      841 acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt agatccagaa
      901 gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg aataccagaa
      961 aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta
     1021 gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt tgatagcgcc
     1081 caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa
     1141 ataaacagct accaaaatcc ggagagtcac aaaacaaagt tatactgtgg agcggaatca
     1201 ttctattaag tatagcaaca atgctttctg caaaaagatt taaacaaaac agatctctct
     1261 aacatttgaa taatgtctgt gaacgcattt ttcaaataaa aagaaatatc cagaaaatga
     1321 ccccaaaata ccagattgaa atctgatatt tggggtcgtt catactttat ctttcttcat
     1381 atttatttta agggatatct gtttcttttg gaagatggtt cgggatcttt ttcactttca
     1441 atacatctaa aaagaaggta aagatcggaa tgccgacaat caagccccaa acgccaaaga
     1501 accgttcgct aacaagtaag accacaaatg tataaaaaat cggcagttct gttttgctgg
     1561 acatcaactt cggattcagc acataagact caattagatg gacaatcaaa atcgtaatca
     1621 ggatataaac aacatctcgg atgcctcctt gggaataagc tatgaaagat agaggaatac
     1681 aagaaatgat aacacctgct actgggatca gacttaaaat gaaaatcatg atcgctaaac
     1741 tcagcagctg atgaaagcca aatacagcca aggcgattgt agtgagcaac gtatttaaaa
     1801 gagcgacaac aaattgagct tctaatacca aaccaaatgt attgacaaac ttatcggcaa
     1861 aatagtaaat atcttgaaag aaccaagaaa attcactttt taaaaacaat cttgagaata
     1921 caattgtttt tttcttttca atcataaaaa agaaacttaa gatgaatgac atagcaaacg
     1981 ccactgccat ttttcctata tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa
     2041 gctgttcaaa aaaatcgttt cgctcaatat aagtatggat atattgtaaa acaggatctg
     2101 catcttttgg ctgatgctcg taaaaattaa taacggactg gatcatgtca aatgtttgat
     2161 gaataagcat cgggatatac tttgtgaatg ccagatagat aaggtaaacg atcagtgtat
     2221 aaatcatgat agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa
     2281 gatgaatcat caaataagta aagataaatg tgagcaaaat ggttgtaatc atacttctaa
     2341 ctaagaaaag aacaaaaacg acaaataata aaacagtgag acggcgtatt ttttcattcg
     2401 ccaaaaaacg ctgataaacg gtcaaatcga caacctccaa ataacaaaaa ataaacacgt
     2461 tattcattat atgatggaat atgaaaataa gaaagagcaa aaagaaaaac cgctaaatga
     2521 cgttatctta tgagttggtt ttcctcataa aatcgttcat ctagcagttt cagccctttt
     2581 tctggtcttg atgaaaatta tctagcattt ttcgggtcag atgttgcagt gtatccaatt
     2641 cttcctgttc aagtggaaga taagacttga tcttttgtgg aataccttct gcttgttttt
     2701 gcaacgcagc accttgagca gtcagcgtaa catttacgat tctttcatca cccgtttcac
     2761 gctgacgatt aatataattt ttttctgcta acttttttag taagggagtc agcgtaccag
     2821 aatccagaca caaatcattt cctagctctt tcactgaagt ctttttcttt tcccataaaa
     2881 ctaacatcac aagatactgg gtataggtaa gattcagcgg cttgaggagg ggagtgtagt
     2941 gtctaatgat ttctttagag gcggcatata aaggaaaaca tacttgatta actaaatata
     3001 gatcttccat ttttccacct ccgctgtttt actatagtgt atcatagcgg atttacacaa
     3061 aaaagtctat tcactaactg ctttcagagt aacttctata tttccagctg tagcttttga
     3121 atacggacac actttatgcg ctgcctctaa taattcttgc gtttcttcca gagaaagacc
     3181 ttcgatagat ccttcaatct ctacacctaa gacaaagctc gtttcacttt ctgaataaag
     3241 ggaaacttgt gcactgacag tagaagcccc agagatattt ttttgctgtt tgatcaattc
     3301 taatgcactg ttaaaacatg cactgaaacc agctgcaaac aattgttcag ggttggttgc
     3361 attttcgacg cgtttaccag gagctgcaat attcaattcg aatgtacgat cgggattatg
     3421 cacttctcca gagcgtccgc ctgtgttgat cattttcgta gaatacattt ttttcataaa
     3481 attaccacct ttaattatat tttatacaat tgaattttac acaatataat tgtggaaagc
     3541 aaagaaatgg attggcataa gataaataag agaaagcaca tgaaacagac gcgctttctc
     3601 gaatagcaaa catatacaaa aaatatcaga gacaaaaaat aatctcatca taaacattac
     3661 atatatttct caaaaagttg ttttctttct tcaaaaatta cttcacgatg gacctgatga
     3721 agcatccata aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc
     3781 atttctgtca tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc
     3841 tcgatatcag agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca
     3901 gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc tcgtaccata caaggtaaaa
     3961 atgacttcat tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag
     4021 attgtttgat acagttctag tgctttcaaa ctatcattta atacaaaatc gatttccgtt
     4081 cctttatgca taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta
     4141 taccagctaa agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt
     4201 ggatgataag taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca
     4261 cttggatcga tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg
     4321 atcaataaaa cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg
     4381 ccacgtccaa tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg
     4441 accataacag atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg
     4501 attgcaatag ataaaatagc ataaagaaac tcttctattc caaaaataaa cagattaaga
     4561 gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt
     4621 ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc
     4681 gcaactccta aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta
     4741 atcattgttt ctggaactat tgcaatgatt aaaggaaaaa ccaagcttcc gaaaagtact
     4801 ttaaaaaagg gttttttacc taaaaataga agagctagta ccaacataac gatattcaac
     4861 actaaaataa ctatagctcg gtcaaagcct aaagcagctt ccagtaatat tcctagaccg
     4921 ctgacacctc cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat
     4981 atgaagaggg caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt
     5041 tattcacttc cttaattcat taataatact cttatcttat tataacttac ttttcacctt
     5101 aagttaactt taattaataa aaaaattagg ctagtctaaa ttattatgtt tcattcttct
     5161 agaatatact tctacgccaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat
     5221 tgatcgctta agataaattc aggacactaa atagtacagg aagtagacta aaacttttaa
     5281 aagttttaag tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg
     5341 ttaccgataa catttttgaa agcgttgtct tttgcttctc tatcacttat agtgatgacg
     5401 tcatattaaa acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa
     5461 agtgaatatg catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa
     5521 aatgatatca aaaaagtata tgtgctatgt ggagatccat ataccataac aactgaaaaa
     5581 tggtatcaaa aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg
     5641 gaaattgaag tgacgagcga aacaagtcgc ttacagtatg cgttccatgt tgttggggaa
     5701 gataacacgg actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt
     5761 actgaaccaa atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt
     5821 ccagaatggg taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg
     5881 gacaaagcaa atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa
     5941 gactttttcg ggggagattt gcaaggtgtg atggatcatt tggattactt gacggacctt
     6001 ggcatcaatg gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat
     6061 acgattgatt attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg
     6121 attgatgaag cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg
     6181 ggtgtccatt caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa
     6241 gactggttcc acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa
     6301 actgctgaaa atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat
     6361 acagcaaatc cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa
     6421 ttcgatattg atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa
     6481 aaattcagag aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg
     6541 cattcctctc aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattt
     6601 acagattcga ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt
     6661 ggaatgaatc atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta
     6721 ttagattctc atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg
     6781 aagtctgtga tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca
     6841 gaaataggga tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag
     6901 gaggaacaag atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag
     6961 ttgtctaaga ttatttctga aggttctact cagtggctaa tagtaaatga tcgtgaagat
     7021 aagctctatt tcacacggaa actagaggga cagatcatct atgtttactt taatcagtca
     7081 aaagaacctt gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg
     7141 ttagaagacg ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg
     7201 ttaccggtaa caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt
     7261 gtaacagcac gttgaataca aaggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt
     7321 tgggtttagg gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta
     7381 actcttcagc agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg
     7441 gatataaaga ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa
     7501 tcaagctagt agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg
     7561 ctggaaaggg acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc
     7621 aaggacatct ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag
     7681 cccaagtgac ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt
     7741 tgtactataa caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc
     7801 tatccaaaga cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag
     7861 ctaaatggac tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat
     7921 ttggtgatga tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag
     7981 gaatctctta tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa
     8041 aaagtgcagg agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg
     8101 acggtccatg gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa
     8161 ttcctactct aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag
     8221 tcagcaatta tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc
     8281 aggaaaatca agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta
     8341 gggaaacagc aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa
     8401 cagctcaagc gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact
     8461 tgatgttcga tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa
     8521 aaacaatcag tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta
     8581 taaaaaatgg aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg
     8641 ctcttgttgc cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca
     8701 gatgcagcaa gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat
     8761 tccagggatg gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat
     8821 attcgtccta ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt
     8881 tactttggga agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt
     8941 gcaattcatc attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta
     9001 tcgagtagcg aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat
     9061 acataatata ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat
     9121 gatttttacg atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga
     9181 cttcaatcat attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag
     9241 tatcttcttt ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat
     9301 ttggacttta tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc
     9361 tcaccagaaa tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc
     9421 ggtcccagct tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc
     9481 catcaatact caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat
     9541 ctcttggaaa acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt
     9601 aggatttccc tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt
     9661 atatgaagca gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct
     9721 tcctgctgtc ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa
     9781 taatttctcc atgatctatc tgtttaacaa tggcggtccc ggaagcgtag gcggaggagc
     9841 tggttctact gatattctta tttcctggat ctataaactt acgactggaa cttctccgca
     9901 gtattctatg gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt
     9961 gctggtattt aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat
    10021 tcgagtgctc gattccaaaa acgactttct cactttttta cttacttttt tcttttaata
    10081 ctgtcagtca ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga
    10141 aatattgcag cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg
    10201 ctattcagtg aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta
    10261 acaatgatcg tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt
    10321 tttatcggcc gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg
    10381 gcagcattga ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg
    10441 ttcttgacaa tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga
    10501 tactttgaca ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat
    10561 ttcaggattt ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca
    10621 ttatgggctt ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca
    10681 cctgaaaatc agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa
    10741 agagtctctc tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc
    10801 ttctttttac aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag
    10861 cagagtatca tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa
    10921 gtgtttgcgg gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta
    10981 gtttccttga tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca
    11041 ttagaacaat ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct
    11101 gaacttgaat tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct
    11161 gaaattttgg taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt
    11221 gattttgaga aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat
    11281 acgatgaatt atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa
    11341 cgatttttag aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt
    11401 ttaagtcttg gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt
    11461 ttcttgtgga tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca
    11521 aatttgatgc tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc
    11581 cactttgact ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta
    11641 tgggtttttg tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt
    11701 tatgcgacaa tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa
    11761 aaattaaact tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt
    11821 ttaaggagga aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc
    11881 tcgctccctg tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac
    11941 actcctcccc aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt
    12001 aacgcatctt tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt
    12061 atccaagcat tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat
    12121 tatctaaaat ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc
    12181 catgcgcatt atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact
    12241 tattcttgtg cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa
    12301 gtccatcata tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt
    12361 tgtggctggg gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg
    12421 atcacactaa gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta
    12481 gaagataaat gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat
    12541 catatcacaa gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc
    12601 aaagtggtaa aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga
    12661 caacaaggcg gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc
    12721 cctggtgtta ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg
    12781 gaaagcttac gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa
    12841 gtccaagatc aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat
    12901 ttacaagctt gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt
    12961 gttcaacctg gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt
    13021 ttaaagacta tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc
    13081 taacttctag gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag
    13141 tttggttctt taatttttaa acatgacaga aaattt
//
LOCUS       DAZUKR010000070        12949 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000070 DAZUKR010000000
VERSION     DAZUKR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.071"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG89C_001765"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG89C_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS4900302.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG89C_001766"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG89C_001766"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4900303.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACG89C_001767"
     CDS             complement(2169..2906)
                     /locus_tag="ACG89C_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4900304.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACG89C_001768"
     CDS             complement(2997..3524)
                     /locus_tag="ACG89C_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900305.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACG89C_001769"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACG89C_001769"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4900306.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACG89C_001770"
     CDS             complement(4401..4886)
                     /locus_tag="ACG89C_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4900307.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACG89C_001771"
     CDS             complement(5039..5236)
                     /locus_tag="ACG89C_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900308.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG89C_001772"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG89C_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS4900309.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG89C_001773"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG89C_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS4900310.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACG89C_001774"
     CDS             9211..9423
                     /locus_tag="ACG89C_001774"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4900311.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACG89C_001775"
     CDS             9425..11104
                     /locus_tag="ACG89C_001775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4900312.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="ACG89C_001776"
     CDS             complement(11358..11558)
                     /locus_tag="ACG89C_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4900313.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="ACG89C_001777"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="ACG89C_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            complement(12086..12748)
                     /locus_tag="ACG89C_001778"
     CDS             complement(12086..12748)
                     /locus_tag="ACG89C_001778"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4900314.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         3784 a         2781 c         2205 g         4179 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tcactttgaa tacatgtatt tgaagtggaa ggtacttatt
    11941 tcaaaaatag taaaacctgt atcttaaatt acttaatagt aacataagat acaggctgat
    12001 tttttattca ttgttattta tactaaagca cccgatagct ctgaaaacaa tcacaaattc
    12061 aacttttcaa agccacagct ttaagttatt ttgtccagac aacccccatt gcccaaccca
    12121 ttttatggaa ttggcatcca ggcaacaact tttcatataa atcgtaataa ttttgttcag
    12181 ataggtactt atctgaagct aaatgctcaa gccatgattt agatgtgttg tgattataaa
    12241 ttctaatcgc attttttatt ccaaatttga aacaatgcgg taaaaactct tggattgccc
    12301 ctaatttata aacataggta ggcggtgtct cgacttcaga tacattatca agaataacta
    12361 ttcttccttc ctcattcaac agttccttca tttgctgtat tacgctggct atatcatcca
    12421 aatgatgaaa ggttgttcgg cttacaataa aatcaaactt ctcattaaaa ttaagttgtt
    12481 ctgcattcat attcagatag accgtatttg ttagttgacg tttagatttg gcaagatcga
    12541 gcatttgatt agaaatatca atccctacca cttcatcata ataacttgct aatttctcca
    12601 ctaacaaacc cgagccacat ccgatatcta atgctctgcc tttctttgga gacatattag
    12661 acacaaagaa tgaataatca ttcaaaagct cattcacgaa atcgtaatct tctgcaacct
    12721 tatcaaactg tgattctatt gtattcaaaa agatccccca ttcctacttt atcgacattc
    12781 tttcattact taccacttta gatgtttttt cgttggggat aaaacttccc tttagacaat
    12841 tttatccaaa gacaatacaa cagtgcaact ttattaaagt cactgtcctt tatcgcagcc
    12901 tttacttttt agtaaagaca gtggcttctc ttatcaagtt tcaaaacat
//
LOCUS       DAZUKR010000071        12943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000071 DAZUKR010000000
VERSION     DAZUKR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.072"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG89C_001779"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG89C_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4900315.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG89C_001780"
     CDS             complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG89C_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4900316.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="ACG89C_001781"
     CDS             complement(3218..5533)
                     /locus_tag="ACG89C_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4900317.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="ACG89C_001782"
     CDS             complement(5718..6578)
                     /locus_tag="ACG89C_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS4900318.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7269)
                     /locus_tag="ACG89C_001783"
                     /pseudo
     CDS             complement(6637..7269)
                     /locus_tag="ACG89C_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7436..8197
                     /locus_tag="ACG89C_001784"
     CDS             7436..8197
                     /locus_tag="ACG89C_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4900319.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="ACG89C_001785"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="ACG89C_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4900320.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG89C_001786"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG89C_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4900321.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="ACG89C_001787"
     CDS             12557..12913
                     /locus_tag="ACG89C_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4900322.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DAZUKR010000072        12778 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000072 DAZUKR010000000
VERSION     DAZUKR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.073"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACG89C_001788"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACG89C_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4900323.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACG89C_001789"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACG89C_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4900324.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACG89C_001790"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACG89C_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4900325.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACG89C_001791"
     CDS             1642..2274
                     /locus_tag="ACG89C_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4900326.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACG89C_001792"
     CDS             complement(2276..3181)
                     /locus_tag="ACG89C_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4900327.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACG89C_001793"
     CDS             complement(3266..3925)
                     /locus_tag="ACG89C_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4900328.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACG89C_001794"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACG89C_001794"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS4900329.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACG89C_001795"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACG89C_001795"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS4900330.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACG89C_001796"
     CDS             6168..7253
                     /locus_tag="ACG89C_001796"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS4900331.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACG89C_001797"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACG89C_001797"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4900332.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACG89C_001798"
     CDS             complement(8563..9366)
                     /locus_tag="ACG89C_001798"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS4900333.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACG89C_001799"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACG89C_001799"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4900334.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACG89C_001800"
     CDS             complement(10244..11251)
                     /locus_tag="ACG89C_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4900335.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACG89C_001801"
     CDS             complement(11258..11902)
                     /locus_tag="ACG89C_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4900336.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACG89C_001802"
     CDS             complement(12002..12760)
                     /locus_tag="ACG89C_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4900337.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
BASE COUNT         3618 a         2706 c         2441 g         4013 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttc
//
LOCUS       DAZUKR010000073        12734 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000073 DAZUKR010000000
VERSION     DAZUKR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.074"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG89C_001803"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG89C_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4900338.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACG89C_001804"
     CDS             complement(322..891)
                     /locus_tag="ACG89C_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS4900339.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACG89C_001805"
     CDS             1077..1979
                     /locus_tag="ACG89C_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4900340.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG89C_001806"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG89C_001806"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4900341.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG89C_001807"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG89C_001807"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4900342.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG89C_001808"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG89C_001808"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4900343.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACG89C_001809"
     CDS             8547..8732
                     /locus_tag="ACG89C_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS4900344.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACG89C_001810"
     CDS             complement(8794..9990)
                     /locus_tag="ACG89C_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4900345.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACG89C_001811"
     CDS             complement(10160..11596)
                     /locus_tag="ACG89C_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4900346.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACG89C_001812"
     CDS             11889..12698
                     /locus_tag="ACG89C_001812"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4900347.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAZUKR010000074        12720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000074 DAZUKR010000000
VERSION     DAZUKR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.075"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..312
                     /locus_tag="ACG89C_001813"
     CDS             163..312
                     /locus_tag="ACG89C_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS4900348.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            323..1837
                     /gene="dltA"
                     /locus_tag="ACG89C_001814"
     CDS             323..1837
                     /gene="dltA"
                     /locus_tag="ACG89C_001814"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS4900349.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1834..3039
                     /gene="dltB"
                     /locus_tag="ACG89C_001815"
     CDS             1834..3039
                     /gene="dltB"
                     /locus_tag="ACG89C_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS4900350.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3084..3317
                     /gene="dltC"
                     /locus_tag="ACG89C_001816"
     CDS             3084..3317
                     /gene="dltC"
                     /locus_tag="ACG89C_001816"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS4900351.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3320..4588
                     /gene="dltD"
                     /locus_tag="ACG89C_001817"
     CDS             3320..4588
                     /gene="dltD"
                     /locus_tag="ACG89C_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS4900352.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4729..5811
                     /locus_tag="ACG89C_001818"
     CDS             4729..5811
                     /locus_tag="ACG89C_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS4900353.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5811..6614
                     /locus_tag="ACG89C_001819"
     CDS             5811..6614
                     /locus_tag="ACG89C_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4900354.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6684..7424
                     /locus_tag="ACG89C_001820"
     CDS             6684..7424
                     /locus_tag="ACG89C_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4900355.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7435..8439
                     /locus_tag="ACG89C_001821"
     CDS             7435..8439
                     /locus_tag="ACG89C_001821"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS4900356.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8484..10328)
                     /locus_tag="ACG89C_001822"
     CDS             complement(8484..10328)
                     /locus_tag="ACG89C_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900357.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            10540..11058
                     /locus_tag="ACG89C_001823"
     CDS             10540..11058
                     /locus_tag="ACG89C_001823"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4900358.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            11186..11869
                     /locus_tag="ACG89C_001824"
     CDS             11186..11869
                     /locus_tag="ACG89C_001824"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4900359.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            12178..12251
                     /locus_tag="ACG89C_001825"
     tRNA            12178..12251
                     /locus_tag="ACG89C_001825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12212..12214,aa:Arg,seq:tct)
     gene            12410..>12720
                     /locus_tag="ACG89C_001826"
     CDS             12410..>12720
                     /locus_tag="ACG89C_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS4900360.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LL"
BASE COUNT         4149 a         2058 c         2560 g         3953 t
ORIGIN      
        1 acgagaaatt ataaagaaaa aagcagtgat ttgatgtgct atttttataa aagctatgtg
       61 acaaaactgt tagtaaaaaa acggtttctg taagctagac acatgtttct ttttgtcaaa
      121 attagttaca ctttgtgtga ggttatatga ggagggaatc caatgaacaa atggcgttct
      181 aatccaaaat tgaattattg gggaaaattc atcattcgaa caatttttta tagtgcagta
      241 ctgctgttcc tgatttatct gtatcactat aaaaatattc aaggaggaac ttttatctat
      301 aacgaatttt aggaggaaga gtatggaaat caaaacgatt attgaagcaa tcgatgattg
      361 gggaataaat gaacctgatc gagttgctta tcaggcggat gagacgcata cttttggtga
      421 attgaaacaa gcatcagacg cacttgctta ttatttagaa aaaaaggtag aaggcgatgg
      481 accaattgtc gtttttggca atttggagtt tgaaatgatt gtctcatttc taggggttgt
      541 caaatctggc catgcctatt tgccgattga agagcataca cccaaggagc gtatcctatc
      601 gatctttcgg gtggctaaac catctatggt catcagtatc ggggactgga tagaagatat
      661 tccttcggtt cctgtcataa caaaaaaaga atttcaagaa atcaagaaga taccagtagg
      721 attttatccc agaaatagcg taaaaggtac agaaaatttt tatattattt ttacttctgg
      781 tactactgga gaaccaaaag gcgtacaaat cagtcatgat aatctagtca gctttgttca
      841 atggacgatg gaagactttg ggatcaagcc tggtatgcat ttcttagccc aagcgccatt
      901 ttcttttgat ttatcggtat tttctattta tcctgcatta gtttctggcg gaatgctgaa
      961 gccgttaaat aaagcagttg ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt
     1021 gaagttgaat gtgtgggttt ctactccatc atttatggat atttgcttga tggaacctac
     1081 attcaatgga gaaaatgtgc cggaattgga catgttttta ttttgcggag aagaattgac
     1141 gaaaaaaact gcagaaagtc tacttgatcg attcccaaat gcacggatat ataatactta
     1201 cggtccaaca gaagcaactg tagctatttc aagtattcag attgaccagc atgtactaga
     1261 tagttatgac cgactaccga ttggctacgt aaaaaaagac acgaaagttt ctattgtcca
     1321 agaaggtaaa cctgtttcaa aaggagaaac gggtgaaatc attattgcag gaccaagcgt
     1381 atcaaaaggt tacctgaaca atcccgaaaa gacagctgct gcgttctttt gtcaaaatga
     1441 cttagcaagt taccatacag gggatgcagg tagactagat gaatatgggc ttttatttta
     1501 tgaaggacgt atggatcagc aggtcaaatt acatggatac cggattgaac tcggggatat
     1561 cgaacattat ctgctccaag acaaccgaat caaacaagct gtcgtcgtac caaaatacca
     1621 aggaacaaaa gtacagcagc tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc
     1681 ggattttcag ttgactaaat ctatcaaaga acaattacta gaagttgtaa tggattatat
     1741 gattcctcag aagttccatt atgtcgatct gctgcctcag acagttaatg gaaaaatcga
     1801 tcgaaaaaaa ttaacagctg aggtgaatcc tgaatgatgt tacctcatat gatcccttat
     1861 gcagagccgt tttatttttt tcttttagcc attgcgcttg taccgatcgt tctatcttta
     1921 ttgatatggg aaaaaagatt accagtttat caatcagcag tgacactttt ctttttattt
     1981 gtcagctttg gaggtaatta ttggcaacag ggagcggcgc tgattgggta tgttttgtgg
     2041 cagacgatac ttgtatgggg ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg
     2101 ttttttgccg ccgttatttt agcgataatt ccactgtttt taacgaaagt gactcctttt
     2161 tttgaacatg gaaaagcatc gttacttggt tttttaggaa tctcttattt aacctttaaa
     2221 tctgtacaaa tgatcatgga aataagagat ggcatgatca aaacatataa gccatttcgt
     2281 tatatccagt ttctgctgtt ctttccaaca atctcttctg gaccaatcga tcgctatagg
     2341 agatttgaaa aagatcagct ccatccgcct gttcgtgata aatatgttga tcttttggga
     2401 aaaggcgtcc aaaatatttt tgtcggtttt ttatataaat tcattatcgg ttattatttt
     2461 ggtcaagtac tgcttcctgt agtgggacgc attgccttac agcatggtgg tatttcatgg
     2521 gcattagtag gttatatgta cgtgtacagt atgtatttgt tttttgattt tgctggctat
     2581 agcttatttg cagtcggtac aagctacatg atgggatatc agacaccaat caatttcaat
     2641 aaaccttttt tatcatggaa tatcaaagaa ttctggaatc gttggcatat gacgctgtcc
     2701 ttttggttcc gtgattatgt atatatgcga ttgatgttct tcatgatgaa gaaaaaattt
     2761 tttaaaagca agatcgtaat gtcaaatatc ggttattttg ctttgtttct tctaatgggt
     2821 atatggcatg gattgacgtg gtactacatc gtatatggac tgtatcatgc ttgtctgatc
     2881 tgtctgacag atgcatggat ccgatttaag aagaagcata aagagcagtt gccttctaac
     2941 aaatttacgc atggcttagc cgtatttttg acatttcaag cagtatgttt tagcttcctg
     3001 attttttcag gatttttaga taaacttttt tttagctaac gaatagtgac atattaattt
     3061 atataacaaa ggagaaaagg aaaatggaag aacaaatttt atcaatctta gaagaaataa
     3121 caggaacaga tgaagtaaga aaaaatcgtg atattgatct atttgatgaa gggttgcttg
     3181 attctttagc atctgttcaa ttattagtcg agctagacgg agagctggat atccaagtcc
     3241 ctgtatctga attcgagcga gaggattgga gcacaccgaa taaaatcatt caacaagcaa
     3301 ctgcattgaa gggttgagta tgaaaaagaa actttttgct attttcggcc cggttgtttt
     3361 agctgttggc ttactgtttg tgttttttac ctcctcctat caagtagata tctctaatcc
     3421 agaaacgatt agacgtgcct cttcttccat gtcagtgaac gtgttgaaag gagatgcgat
     3481 aaaaaattat gcattatctg aaaaacaata tattcctttt tttggctcaa gtgaattaag
     3541 cagaatcagt ccatttcatc cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc
     3601 atttttgctg ggagcacccg ggacacaatc gttaagccaa tatatgatga tgcgttctgc
     3661 aggagatgct atgaaaaaca agaaagtcgt ctttattatt tctcctcagt ggttcgttaa
     3721 aaatggtgta aaaactgatt actttaatac gtattattct gaacttcaaa catacgactg
     3781 gttgttttca atgaagaaag taacgcctgc tgatcgctat ttagctagaa gattattaac
     3841 tttctcaaaa gtaaaagaga atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg
     3901 gaagctacct ttacctgaat ctttgaatca gctgcgcagc caatggaaca tgctgaagag
     3961 agaggatgaa gtattcagca atattgggtt gaaagatcga cagcaaaaaa tcgatcatga
     4021 gtcaaaacga ttaccaaaac aataccaaga aacagaatta tccattttag caaatcaaat
     4081 tggagaacga gaaacgacga ataatccatt cgggttgaaa aacgattttt atacgcatcg
     4141 tatccgagcc cacgagccag agctcaaaca gtcacaaaaa aattgggatt atcgtttttc
     4201 gccagaattt tctgattttc agctggtctt ggatcaatta gcaaaaaacc ataacgaagt
     4261 attgttcatc atcccaccgg ttaacgagaa atggagcgat tataccggac tatctcaaga
     4321 aatgttacaa ggatttgcca aaaagatcaa atttcaatta aatagccaag gattcaatcg
     4381 aatagctgat tttgtcaatc aagcgggtac caactatttt atggaagaca cgatccatct
     4441 aggctggaaa ggctggttgg ctgcagatca acagatcaga ccgtttttag aagaaaatca
     4501 tattactgct agtaagtatc atcttgacga tgcttttttt agcaaatctt ggcaacatca
     4561 aataccagat aaactacaat taaaataaag aaaacaggcg agtgttgctc gcttgttttc
     4621 tttatttttg aatattctga ataaaagata aaaataccaa gtttgcctga acaaaaatca
     4681 taagcaatgg tataatcaca atatgaatat agaaatgggg gaaaacattt ggatacaaaa
     4741 gaagaacagc tacttattga tttaacttct gcaaaaggag tacctggaaa tgaagaagag
     4801 gtaagagaag tattcagaga atatgccaaa ccattcgctg atgatatttt ttatgacggt
     4861 ttaggcagtg tgattgccaa acatggtgct ggcggaggcc ccaaggtctt tatttctgga
     4921 catatggacg aagttggttt tatggtaaca aaaataaccg aaaaaggatt cttggaattc
     4981 caaactcttg gaggttggtg ggggcaagtc atgcttgctc agcaagttga aatcaaaaca
     5041 cgtgaaggga aaatcgtcca cggtgtgatt ggttccaaac caccacatgt tttaacacct
     5101 caagtaagaa ataaacctta tgaaataaag gatatgttca ttgatattgg cgcatcaagc
     5161 caagaagaag ccaaagagtg gggcatacgt cctggagata tggtcactcc atatattgaa
     5221 tacaaacgaa tgaatggcag taaatattta ctggcaaaag catgggataa tcgtatcggg
     5281 acagctgttt ctctgcgagt attagagaat ctatctaaag aagcacaccc aaatgtttta
     5341 ttcgcaggaa gcgatgtgca agaagaagta ggcttgcgag gtgctcgaac aagtacacac
     5401 cttgtaaatc ccgatattgc ttttgcttta gatactggta cagcaggaga cactccagga
     5461 atgacaccaa aagaagcaga ttcgatttta ggaaaaggcc cacagatttt gatttttgat
     5521 gcatctatga tcccgcataa aaaattattg aattttgtta ttgatgtagc agaggaaatg
     5581 gatattccat tccaatatac agtgatttct ggcggaggaa ctgatgcagg acaaatgcat
     5641 ttgacaagag atggtgttcc gtctgtagcc attaccgttc ctgttcgtta tctgcattcc
     5701 catacatcag tgattcatga agatgattat ttgaatacag tgaaactagt cacagaatta
     5761 gtgaaacggc ttgatagtga aacagttgaa aaaattcgga gttatgaata atgaataata
     5821 gaatacctat ttctgaatat cggaaaaaag cacgagtttc tttggaaaat caatggggaa
     5881 tcaatacttg gcttgttttc ttatctataa tatttacgag cgtcatccag aacttttttg
     5941 acggtttcag tccttttccg gaagaaagca tgcagcagca cttattgagt tttctagtcg
     6001 ataatttact tttattcgca ttcaactatg cattgtttta cattgcgcta gtagttgtac
     6061 ggggtggcag accaaaaagt aaccttttat tttcagtttt ccaaagaaag tattttggtt
     6121 cgatacttgg catcaatttg atcaattcag tcatcaactg gctactcaac agtttaatct
     6181 ttctgcccat gtttttaatc agcggagtga gtacttatat gcagctgctt ttcagttcaa
     6241 atgcggtacc tacgggtgtc atgccagaag attttcttga tatcagcttt gttttatcga
     6301 tgatagggat gatttttgtt tcagcactca tcatgagtat tgttggcggt ttattccaat
     6361 ttgctgcatg gacaaagttc gactatcctg atttgtcggt tattcaatgt ttgaaatatg
     6421 gctggttttt attgaaagat agaattggta cgtatatttt gttgcagctg tcttttatcg
     6481 gctggtattt gctaggagca atcgctttgt tcatcggtct tttgtgggtc attgtttatg
     6541 tgaatgtgtc agttgcagaa ttttatgaac aagcacggat tgaaaaagga ggtccagacg
     6601 cctattttac ttagcatgta agcagtttat tcatcaaaaa aataactttt gtggtaggat
     6661 aaatctttga gggggaatgt acgatgaaat taactgtttt aggatgctta ggagcgtatc
     6721 cttataaaaa tcaagggaca acaggttatt tgcttcaatc tgacgggttc aatttgttga
     6781 tcgatgcagg tagtgctacg ctgatcaaat tacaagaaca ccttgatcca cttgatctag
     6841 atgctgtgat ccttagccat tatcatcatg atcatattgc tgaccttggt gtattacagt
     6901 attactggca acttcatcct aagagaaatc cggagacatt gctttcaatt tatggacaca
     6961 caaaagattc cattcacttt gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt
     7021 attttgaagc tgaagaatta aaaattggtc cttttctgat tacttttatg gaaacaatcc
     7081 atccagtagt atgttacgcc atgcgtatcg tagaagaaac aacggggaaa gtattcgtat
     7141 ttacaggtga ttcaggttat ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt
     7201 ttctagcaga tacctatctt tttgaaggaa atgaacatca tcatgcccat ttcacttcaa
     7261 aagaagctgg agaacttgcg aaatcagcta acgtaaagaa acttgtactt acacacctac
     7321 ctcagtttgg tgatttagaa caattgaaaa atgaagcgca aaaggcagca ggcaaaggga
     7381 ttgatataga acttgctgca gttgacaaag tgtttaacat ttaggaggaa gaacatgatt
     7441 tttgtaccaa atgaaaacca cgacccaaga atcaatttag caattgaaca atatttgctg
     7501 caagaaatgc ctttagatga acctattttg ctcttttata tcaatgaacc ttcaatcatc
     7561 atcggcagaa accaaaatac gcttgaagaa atcaatcgag attatgtgga agaaaatggc
     7621 attcatgttg tccgtcgctt aagtggaggc ggagctgtct accatgattt tggaaacctg
     7681 aactttagtt ttatcatgcc tgatgatggg gattcattcc gtgactttgc gaaagtaaca
     7741 cagccaatca tccaagcact tcatgaattg ggtgttgctg gggcggaatt gaaaggccga
     7801 aatgatctag tcattgatgg catgaaattt tctggcaacg cgatgtatgc taccaacggt
     7861 cggatgtttg ctcatggaac aattatgttc gactctgata tcaatgaagt agtcaatgca
     7921 ttaaaagtaa aaaaagacaa aatcgaatca aaaggaatca aatcgatccg ttctcgtgta
     7981 acgaatatca aaccatttct tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt
     8041 caagaaatct tgttgaaaat tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa
     8101 ttgactgatg aagattggaa aaaaatcaat cgaatctctg aggaatatta tcgaaattgg
     8161 gattggaatt acgggaagtc tccagatttc aatttctctc gccaaaaacg tttttctatc
     8221 ggttcgattg aagtccattt gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt
     8281 ggcgatttct tcggactagg agatatctct gacgtggaaa aaaaattggt tggacagaaa
     8341 tatgataaag catctctatc aaaaatagtc agccaaattg atgtgaagaa atattttggt
     8401 gcgatagaac cagaagaatt gcttacctta ctttattgaa aataaaaaat agcggaatcc
     8461 acatatatgg attccgctat tttttataca ggattcgggt aatttcgaga gagttgaaca
     8521 gtgatccgat caatgaaagt atctttttcc tgcgttagtg ttcctgtgta acgatccgct
     8581 acgtctttca tcatcattaa gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct
     8641 cgactaaata ggtttgtatc gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc
     8701 tcttgttcgt gtatcgacaa ataaattttt cgatacagtg gattttcgat ggcatgagtt
     8761 tctctaaagg aatgctctaa agcgtggatc aaaagttgag ccagatcgtc atcgttgata
     8821 ggaagttctt cgggaagatc tgcttggaag gttatctgaa tattccgttt ggcagctaac
     8881 ttctggtaac gcgctaagat aagatttgtc acctggtggt tgcaaaaaga acacagcatt
     8941 tcgaaatttt ttgcttcttc taatatcttt tccacatagt ttttagcttt tgtttggtcc
     9001 gtatgcatta aatcttgcag attctccatc tcttggacaa attccaggcg aagtgtattt
     9061 acttcttgga tatgctgtcg aagctgctca taatgttgct ccaacaaatc atttttgatt
     9121 tttaagaaat ctacttgtct ccgatctttg tcggtattta agatgtgatc gattagattg
     9181 tagctgataa taacgattag aatgataata tataaaatcg tagaccaatt gatcgttgtt
     9241 tgatagatac ctaatgccgt ttggatgtaa atcaagcaat ggataatggc taccaagacg
     9301 atccaaggtg tacaagctac ataaaaacgg ttattcttat atgcttcgcc aatgcatgca
     9361 agtgaagtga ctaaagtgct tataacacta gcaatattag caatcggcat taattcagga
     9421 agttctgcag gagtaaaagt tgcataaagc agaacgccga taaaaatcaa aagttggaac
     9481 gtacaaaaaa ttccgtacca ttttttatag taaaacattc ggctccaata gtagcagatg
     9541 atgaaagctg acgttaagat aataaataaa tgagataaaa aagaatgaac gaatggttca
     9601 aaaagaatac cacttaaaat atcttctgat acagcttcaa atcctaatgc caaagcaaag
     9661 caacttaaga gaataagtga gtattcaaga cgtttttgtt tatacatcaa gtagatcgta
     9721 tagaaaaagg tccctatagc gataaaaaca gcgattatca tcgtcaatac ttgcggcact
     9781 gattgtgaat aaataaaact aatcaatggt ccagttgttc ctagataaac tttcggtggc
     9841 agtccagcat agttctgata aggagatgag actgtaaggg tcaatttttt tccgatataa
     9901 tttgctggta aatctatcac agccaatgag agtccgggat tgctttcttc ctgataatct
     9961 ttccgttggt ataaaatctt ttcgcctaga tatacagtaa tccattgatg gtttcctttg
    10021 attagtaaat ttgcttcgtc caacggtttt tcaagtgtcg tttccataat gaatgtttcg
    10081 tctttatcca cggtaggaat cgtacgagtg tacttgctgt caaacttaaa atcaggatca
    10141 tcttttgtat agaaaaccca cgaatctttt atttctttca tttgattgct gtccgcagga
    10201 gaaaaaataa aataaaagaa ataagcaatt cctgttaaaa ataacaatga taatataatg
    10261 attatgctcc actttatacg taaataaaaa gattcttttt tattatatat gttattaaaa
    10321 aaagacataa aggtattcct ttctaaaact tgtaatgtta gggtaaacgt tttttaaact
    10381 aacgtcaatt atttataata aaaaatgaga taaagatgag gaaatagaat tagaaacata
    10441 taattattaa agggaaacat ttttacagct ataatttgaa aaaaatttat cgttattata
    10501 tgattaagaa ggattgaata aagaaagaag ttgaaaacga tacctgatta caaaaaaata
    10561 acttttataa gttctataac gttgattata tttttaattc ttattcttct cgtcgtcttg
    10621 gttccccgaa tttttggttt gactccttat attattgagg acaaagaaat gagtccatat
    10681 tatgcacaag gaaatctgat atatgtacga ccgaaaaata caaatgagat tttagtcgga
    10741 gatattatta catattatga aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc
    10801 gttgaaaaca accacgaaga tttttataca aaagctgata caaagactta tattgagcct
    10861 ggagtagtaa aaaagagaaa tattatcggt agtcccatct ttcagattcc atatattgga
    10921 ctagtccttt caaaaacggc atttgcatgg attcaggggt tattctttac gattgctttt
    10981 tgcctaacag gaatcacagc atggaatacc tttgtagagt tgaaaaagaa gcggagacga
    11041 acacagaaat ttttgtaaga atgttaaaaa cataaatgta ttccagagat ccaacgactc
    11101 ttctgaacat gaaacacgag aaaacactca tgcagcttgt agatttagtc agttgttcgt
    11161 ataatgtgtg aagaggagga gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa
    11221 agaagaacta ttggatttta tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa
    11281 actggaagaa gaagctcatg tagcagaagt accagttatt ccccatgaaa cagttgtatt
    11341 tttgaaattt ttacttggac agcttcagcc tgaacgaatc ttggagatcg gagcagctat
    11401 cggtttttca tcaagtgtca tggccacgac aatcggagaa aacgctcatg ttacgacaat
    11461 tgatcgtttc gatgtcatga tcgagaaagc taaaaaaaac tatgaacgtc taggactgac
    11521 agataaagtg actttgcttg aaggtcaagc agcagatatc ttgccggagt tgtctggtcc
    11581 gtatgacttt atttttatgg atagtgcaaa atcaaaatat attgaatttt tacctgaatg
    11641 cttacgtttg ttaagaaaag gtggcgtttt gatggtagat gacatcttcc aaggaggaac
    11701 gatcttgctc ccagatgaag agattccccg tggcaaacga gctatccatc gtaaattaaa
    11761 tgaatttctt cgtgttgtga tggatcatcc cgatttgaca tcgacaatct tgccattagg
    11821 tgatggtgtg atcttaatga caaaggaatc agaaacaatc gagttgtgat acatgattat
    11881 ggcagcctgt agataatcga ttgaatcgag ggctcaattc gaacttgatg taaaagtttc
    11941 tatatttgta agtcaattaa aggatacagg aaatctaaat aaacaggagt agcatgaagt
    12001 caatgtagct cctgttttca attgataacc ttagaacagc tgaaggagaa tgtctcgttt
    12061 taaaagattg atttagtagt tgggccaaaa taattcatta tttgctaatt aaataactat
    12121 ttcgtattga cagaagcaat ttaattatct ataattattg atgttccgct gattcttgtc
    12181 atagtagctc agctggatag agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc
    12241 ctcctgtgac gctaaaaaac cgttagaaac tgaggtttct aacggttttt ctttgttttt
    12301 tgtgccaata ttgtttgttt tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac
    12361 aattataagg agcaaaaaaa taaaactcat cagaaaagag gaagaagata tgacgatact
    12421 atctagaatt ttagtgactc tagttgcaat tgaatttttt tatatcatgt atatcgaaac
    12481 ggtgagaact gattctgaca cgacaagtag ggtttttaaa atgagtaaag aagagttaag
    12541 cagaaaatct gttcaaacat tgtttaagaa ccaaggagtg tacaatggac tattaggagt
    12601 gggactactt tatggagctt atctttcttc cgcatctaaa gaaatcacaa gtatgctatt
    12661 aatatctatt ttttttgttg cattatatgg cagtttgact agcgatcgtt ggattttact
//
LOCUS       DAZUKR010000075        12457 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000075 DAZUKR010000000
VERSION     DAZUKR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.076"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..428)
                     /locus_tag="ACG89C_001827"
     CDS             complement(66..428)
                     /locus_tag="ACG89C_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900361.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(502..882)
                     /locus_tag="ACG89C_001828"
     CDS             complement(502..882)
                     /locus_tag="ACG89C_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS4900362.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            964..2139
                     /locus_tag="ACG89C_001829"
     CDS             964..2139
                     /locus_tag="ACG89C_001829"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4900363.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2216..2800)
                     /locus_tag="ACG89C_001830"
     CDS             complement(2216..2800)
                     /locus_tag="ACG89C_001830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4900364.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3001..3999
                     /locus_tag="ACG89C_001831"
     CDS             3001..3999
                     /locus_tag="ACG89C_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4900365.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4111..4620
                     /locus_tag="ACG89C_001832"
     CDS             4111..4620
                     /locus_tag="ACG89C_001832"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4900366.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4650..5309)
                     /locus_tag="ACG89C_001833"
     CDS             complement(4650..5309)
                     /locus_tag="ACG89C_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS4900367.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5311..6075)
                     /locus_tag="ACG89C_001834"
     CDS             complement(5311..6075)
                     /locus_tag="ACG89C_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS4900368.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6083..6760)
                     /locus_tag="ACG89C_001835"
     CDS             complement(6083..6760)
                     /locus_tag="ACG89C_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS4900369.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6797..8182)
                     /locus_tag="ACG89C_001836"
     CDS             complement(6797..8182)
                     /locus_tag="ACG89C_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS4900370.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8392..8476)
                     /gene="ffs"
                     /locus_tag="ACG89C_001837"
     ncRNA           complement(8392..8476)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG89C_001837"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8499..9014)
                     /gene="tadA"
                     /locus_tag="ACG89C_001838"
     CDS             complement(8499..9014)
                     /gene="tadA"
                     /locus_tag="ACG89C_001838"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4900371.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9121..9978
                     /locus_tag="ACG89C_001839"
     CDS             9121..9978
                     /locus_tag="ACG89C_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900372.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10059..10394)
                     /locus_tag="ACG89C_001840"
     CDS             complement(10059..10394)
                     /locus_tag="ACG89C_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900373.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10461..11831)
                     /locus_tag="ACG89C_001841"
     CDS             complement(10461..11831)
                     /locus_tag="ACG89C_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS4900374.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11909..12445)
                     /locus_tag="ACG89C_001842"
     CDS             complement(11909..12445)
                     /locus_tag="ACG89C_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4900375.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3691 a         2541 c         2098 g         4127 t
ORIGIN      
        1 ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct
       61 gtattttaga agaaaggcac tccctctact tcgtaagttc caagaacaga gcgcatttga
      121 cctggttctt cccaaatata tccttcgcct aattcttctc tttgattctt ttgttcagtt
      181 tcaaagacaa acaacggaac attttcctct ttaatatgtg tatttgtcaa ttccatcaaa
      241 ctaatcttgc taacatcgat atgaactatt tcttttaaga aattaagtat attcgctaaa
      301 ccagtctttc cttcagaaag ttccgctaac gcaaattcca acttgtcatc aattgaatgg
      361 accaaaaatt ttttagtacc atcctccaaa tgcaacataa ttgtaccagc aactcttttg
      421 cttgccaaaa tttccacctc gatcaacttg ttctatggct tacataaaaa ttctagcaca
      481 ttttcagaaa aaatacactt tttatttctt attttctttc cgttctagtt cagataatgc
      541 ttcttctatc caaataggta actgctccgc aatagtggta aatccgatct tacgattttt
      601 attcgtgaaa tacctttttc tgcgatatgg aacagctgga tttgtttttt ctaacgttct
      661 taaagataaa ctgatttttt tggaatattc atcaatgtcg atgatctgaa ctgttactgg
      721 ctgtccgact tttaacaatg aatggatatt tttcgtataa ccggattgta cttctgaaac
      781 atgaataagt ccttgcgtct cgttatcaag agaaacgaat gcgccataag gctgtatccc
      841 tgtcactttt ccttcaatta tttgtccaat tttataagtc atctacttcc ctctcttcgc
      901 atttatcatt atactgtaaa tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt
      961 aagatgatcg aatttgataa acagatttca agaaaagata cagaaagtgt aaaatgggac
     1021 gcaattgctg agacatatca aatggatgac ttacttcctt tgtgggtagc agatatggac
     1081 ttcttgtctc ccggtggggt agctaaagcg tttagtgaat acatcaaaca tggaatcttt
     1141 ggttatgcca ctctgtctga taaactatat caggctatca ttcattggga acgccaacac
     1201 catgctgtgg agttgacgaa agaaaatatc gtttttacaa gtggtgtgtt aaccagttta
     1261 gctgcagcag tacaaacttt tacaaaacca ggcgattcta ttttgattca tgacccagtt
     1321 tatcctccat tttcctctat tatagaaaca aatcaacgtc acatcgttcg aagctctttg
     1381 ctagaaaaaa ataatcattt catcatggac ttgtctgaca tggagaaaaa aatcgaagaa
     1441 aacaatgtaa aagcaatgat tttatgcaat cctcacaatc ctggagggcg tgtttggagc
     1501 aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac atcaagtgat tctgtttagt
     1561 gacgagatcc atcaagattt aactttattc gatcatacat ttacttccat gctaacgatt
     1621 gatcctaatt tagatgatct actagttgct tttacttccg caaccaagac tttcaatcta
     1681 gctgcaatca aaaattcaat ggtatttatc aaaaatccag agatgaaagc tgattttgaa
     1741 aaacatttag tgatgaacca acagcacgaa atcaacactt ttggactgat tggaacccaa
     1801 gcggcttatg agacgggtgg agaatggtta agccaactga ttccttatct agaaaaaaat
     1861 gttcaaacag ttaatcagtt ttttgaacag tatcttccga aagtgagaat aatgaaccct
     1921 gaaggaactt acttaatgtg gttagatttt accgcctata tttcagacga ccaagaattg
     1981 gagaacactc ttgttcggaa aggaaaagtc gtattaaatc ctggcatcac gtttggtcct
     2041 tccggtcatg ggcatatgcg cttgaatatt gcttgtccag aagaaacttt actggaagga
     2101 cttaatcgaa tcaagtatgc tttagagaca atcgattaaa tcatttattc aaaataaaag
     2161 gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt
     2221 ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt
     2281 gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat
     2341 gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga
     2401 atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa
     2461 agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt
     2521 tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca
     2581 ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa
     2641 tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg
     2701 ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg
     2761 gtcctttttc actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt
     2821 gcatacttca taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt
     2881 ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa
     2941 acatttttga gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt
     3001 atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt
     3061 tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt
     3121 caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata
     3181 aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc
     3241 catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca
     3301 aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag
     3361 ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc
     3421 gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct
     3481 gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt
     3541 cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac
     3601 ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc
     3661 cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc
     3721 aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga
     3781 aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt
     3841 gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct
     3901 actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac
     3961 agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt
     4021 tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag
     4081 agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa
     4141 aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc
     4201 atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc
     4261 aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat
     4321 atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa
     4381 ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc
     4441 ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac
     4501 agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct
     4561 gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa
     4621 ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc
     4681 aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat
     4741 aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg
     4801 ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga
     4861 tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac
     4921 ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc
     4981 gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa
     5041 aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag
     5101 ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa
     5161 gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg
     5221 atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat
     5281 tccatccact gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt
     5341 cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa
     5401 aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc
     5461 tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat
     5521 gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga
     5581 gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt
     5641 cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata
     5701 ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa
     5761 tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc
     5821 ttttccatct gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg
     5881 agctacatga atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt
     5941 cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg
     6001 tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata
     6061 tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg
     6121 ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact
     6181 ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct
     6241 catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt
     6301 ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa
     6361 gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga
     6421 aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg
     6481 ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg
     6541 acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta
     6601 gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca
     6661 gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt
     6721 tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt
     6781 ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac
     6841 ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa
     6901 atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc agatcgattg
     6961 gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca
     7021 tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt
     7081 tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat
     7141 ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt
     7201 ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt
     7261 ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca
     7321 gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat
     7381 aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt
     7441 tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat
     7501 actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga
     7561 ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt
     7621 ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta
     7681 aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat
     7741 tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg
     7801 ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata
     7861 agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg
     7921 aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca
     7981 gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat
     8041 cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt
     8101 gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat
     8161 gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag
     8221 gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa
     8281 ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt
     8341 acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca
     8401 gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc
     8461 caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag
     8521 tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc
     8581 ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc
     8641 tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa
     8701 acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta
     8761 acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt
     8821 gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt
     8881 ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct
     8941 gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga
     9001 tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg
     9061 ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag
     9121 atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca
     9181 tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat
     9241 tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa
     9301 aaaaaacacc catggtatcg ctatcttttt aaagatgctt cttgtgttca agaaaaaagc
     9361 tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa
     9421 gatactattt tggaattatt tcgctattct gctgatttct ttttttatac agacacagat
     9481 gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg ctactgaaat caacggtatt
     9541 tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat
     9601 tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag
     9661 caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac
     9721 tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa
     9781 caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct
     9841 gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa
     9901 gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg
     9961 cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc
    10021 cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa
    10081 tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag
    10141 attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta
    10201 tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg
    10261 aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat
    10321 acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt
    10381 gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa
    10441 aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact
    10501 gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat
    10561 ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga
    10621 gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta
    10681 ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa
    10741 ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt
    10801 cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct
    10861 tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt
    10921 ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata
    10981 cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat
    11041 tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc
    11101 tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt
    11161 agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat
    11221 tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa
    11281 ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt
    11341 gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc
    11401 ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca
    11461 gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt
    11521 tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca
    11581 gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga
    11641 gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt
    11701 ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat
    11761 gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct
    11821 tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg
    11881 tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca
    11941 agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt
    12001 gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact
    12061 tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg
    12121 aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa
    12181 aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt
    12241 tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga
    12301 aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa
    12361 ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac
    12421 tataatttgt tactctcccc atcattctta cctccgt
//
LOCUS       DAZUKR010000076        12213 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000076 DAZUKR010000000
VERSION     DAZUKR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.078"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG89C_001843"
     CDS             99..746
                     /locus_tag="ACG89C_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4900376.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG89C_001844"
     CDS             1139..3784
                     /locus_tag="ACG89C_001844"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4900377.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG89C_001845"
     CDS             4094..5407
                     /locus_tag="ACG89C_001845"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS4900378.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG89C_001846"
     CDS             5397..6050
                     /locus_tag="ACG89C_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4900379.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="ACG89C_001847"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="ACG89C_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="ACG89C_001848"
     CDS             6787..7872
                     /locus_tag="ACG89C_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4900380.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="ACG89C_001849"
     CDS             7869..9761
                     /locus_tag="ACG89C_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4900381.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="ACG89C_001850"
     CDS             9768..10145
                     /locus_tag="ACG89C_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFS4900382.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="ACG89C_001851"
     CDS             10299..11081
                     /locus_tag="ACG89C_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS4900383.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="ACG89C_001852"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="ACG89C_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS4900384.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="ACG89C_001853"
     CDS             complement(11996..12055)
                     /locus_tag="ACG89C_001853"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS4900385.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
BASE COUNT         4200 a         1892 c         2512 g         3609 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttg
//
LOCUS       DAZUKR010000077        12205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000077 DAZUKR010000000
VERSION     DAZUKR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.079"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACG89C_001854"
     CDS             complement(37..216)
                     /locus_tag="ACG89C_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900386.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACG89C_001855"
     CDS             complement(232..432)
                     /locus_tag="ACG89C_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900387.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACG89C_001856"
     CDS             complement(597..1304)
                     /locus_tag="ACG89C_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900388.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACG89C_001857"
     CDS             complement(1297..1734)
                     /locus_tag="ACG89C_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900389.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACG89C_001858"
     CDS             complement(1749..2000)
                     /locus_tag="ACG89C_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900390.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACG89C_001859"
     CDS             complement(2000..2206)
                     /locus_tag="ACG89C_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4900391.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACG89C_001860"
     CDS             complement(2643..2903)
                     /locus_tag="ACG89C_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900392.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACG89C_001861"
     CDS             3077..3181
                     /locus_tag="ACG89C_001861"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS4900393.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG89C_001862"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG89C_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4900394.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACG89C_001863"
     CDS             complement(3810..4709)
                     /locus_tag="ACG89C_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4900395.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACG89C_001864"
     CDS             complement(4722..5315)
                     /locus_tag="ACG89C_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4900396.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACG89C_001865"
     CDS             complement(5391..5540)
                     /locus_tag="ACG89C_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900397.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACG89C_001866"
     CDS             complement(5556..7760)
                     /locus_tag="ACG89C_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4900398.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACG89C_001867"
     CDS             complement(7771..9231)
                     /locus_tag="ACG89C_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4900399.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACG89C_001868"
     CDS             complement(9292..11664)
                     /locus_tag="ACG89C_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4900400.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            12074..>12205
                     /locus_tag="ACG89C_001869"
     CDS             12074..>12205
                     /locus_tag="ACG89C_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4900401.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         3040 a         2531 c         2053 g         4581 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa ggatccaacg
    11821 ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca
    11881 ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt
    11941 ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata
    12001 cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg
    12061 aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt
    12121 ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga
    12181 catcgaaggt gcttcattca tacga
//
LOCUS       DAZUKR010000078        12189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000078 DAZUKR010000000
VERSION     DAZUKR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.080"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="ACG89C_001870"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="ACG89C_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4900402.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACG89C_001871"
     CDS             complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACG89C_001871"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4900403.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACG89C_001872"
     CDS             complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACG89C_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4900404.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACG89C_001873"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACG89C_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4900405.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="ACG89C_001874"
     CDS             3621..4100
                     /locus_tag="ACG89C_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4900406.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="ACG89C_001875"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="ACG89C_001875"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS4900407.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="ACG89C_001876"
     CDS             5401..5529
                     /locus_tag="ACG89C_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS4900408.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5533..6078
                     /locus_tag="ACG89C_001877"
     CDS             5533..6078
                     /locus_tag="ACG89C_001877"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS4900409.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACG89C_001878"
     CDS             complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACG89C_001878"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4900410.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACG89C_001879"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACG89C_001879"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4900411.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACG89C_001880"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACG89C_001880"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4900412.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACG89C_001881"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACG89C_001881"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS4900413.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3338 a         2527 c         2202 g         4122 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagc
//
LOCUS       DAZUKR010000079        11741 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000079 DAZUKR010000000
VERSION     DAZUKR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.081"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG89C_001882"
     CDS             complement(105..629)
                     /locus_tag="ACG89C_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS4900414.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG89C_001883"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG89C_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4900415.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG89C_001884"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG89C_001884"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4900416.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG89C_001885"
     CDS             complement(2351..3031)
                     /locus_tag="ACG89C_001885"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4900417.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG89C_001886"
     CDS             3157..4026
                     /locus_tag="ACG89C_001886"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4900418.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG89C_001887"
     CDS             4052..4567
                     /locus_tag="ACG89C_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS4900419.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG89C_001888"
     CDS             complement(4614..5177)
                     /locus_tag="ACG89C_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900420.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG89C_001889"
     CDS             complement(5232..5411)
                     /locus_tag="ACG89C_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900421.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG89C_001890"
     CDS             complement(5488..5997)
                     /locus_tag="ACG89C_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900422.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG89C_001891"
     CDS             complement(6093..6473)
                     /locus_tag="ACG89C_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4900423.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG89C_001892"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG89C_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4900424.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACG89C_001893"
     CDS             complement(7736..9013)
                     /locus_tag="ACG89C_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900425.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACG89C_001894"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACG89C_001894"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS4900426.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACG89C_001895"
     CDS             complement(9935..10879)
                     /locus_tag="ACG89C_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS4900427.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="ACG89C_001896"
     CDS             complement(10881..11567)
                     /locus_tag="ACG89C_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS4900428.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3353 a         2478 c         2056 g         3854 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga t
//
LOCUS       DAZUKR010000080        11462 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000080 DAZUKR010000000
VERSION     DAZUKR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.082"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..609)
                     /locus_tag="ACG89C_001897"
     CDS             complement(64..609)
                     /locus_tag="ACG89C_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4900429.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(621..1313)
                     /locus_tag="ACG89C_001898"
     CDS             complement(621..1313)
                     /locus_tag="ACG89C_001898"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4900430.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1332..1616)
                     /gene="macP"
                     /locus_tag="ACG89C_001899"
     CDS             complement(1332..1616)
                     /gene="macP"
                     /locus_tag="ACG89C_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS4900431.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1629..2192)
                     /locus_tag="ACG89C_001900"
     CDS             complement(1629..2192)
                     /locus_tag="ACG89C_001900"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4900432.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2420..3082
                     /locus_tag="ACG89C_001901"
     CDS             2420..3082
                     /locus_tag="ACG89C_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS4900433.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3206..4402
                     /locus_tag="ACG89C_001902"
     CDS             3206..4402
                     /locus_tag="ACG89C_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS4900434.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4503..5879)
                     /locus_tag="ACG89C_001903"
     CDS             complement(4503..5879)
                     /locus_tag="ACG89C_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4900435.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5916..6782)
                     /locus_tag="ACG89C_001904"
     CDS             complement(5916..6782)
                     /locus_tag="ACG89C_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4900436.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6871..7896)
                     /locus_tag="ACG89C_001905"
     CDS             complement(6871..7896)
                     /locus_tag="ACG89C_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4900437.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7914..8588)
                     /locus_tag="ACG89C_001906"
     CDS             complement(7914..8588)
                     /locus_tag="ACG89C_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4900438.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8772..9665)
                     /locus_tag="ACG89C_001907"
     CDS             complement(8772..9665)
                     /locus_tag="ACG89C_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4900439.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9739..11436)
                     /locus_tag="ACG89C_001908"
     CDS             complement(9739..11436)
                     /locus_tag="ACG89C_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4900440.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3388 a         2518 c         1968 g         3588 t
ORIGIN      
        1 gtttgtttta tgctcttctt ttattatttt gtgcttttta ttttatcaaa tcactttgtt
       61 tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc
      121 aacgggatcg aaacttgcca cggcatcttt gaaaatatgg agtttgtatc ctagattata
      181 cgcatcgact gctgtatgca acacacagat atctgtacat acgccggtca aataaatatc
      241 tgtgatctgg cgttctcgca aacggatgtc tagatctgtt ccactaaaag cggaataatg
      301 ccgtttgtct atccaataaa cattttcctc ttgctcatag tcttggtata aactttttaa
      361 agagccgtac aattcccgtc cacccgttcc tatcacatta tgcggaggaa ataagcggtt
      421 ctccggatga aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt
      481 ttgatcaata aacttctttg tatgagcagt cagctttttt tcaattactt gtcctgctgt
      541 cccagtagtc agctttccat cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa
      601 tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa attcgatcac catttctgct
      661 gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga gtgatctgct
      721 gtgtcgctca ttgcacgtac gatcaaaaat gggatattga actgacgagc tgtttgtcca
      781 acagcggctc cttccatttc gcaagctaat gcttcaggga aattcgttaa gatttctttg
      841 attttatctg gcgagtcgac aaatgtatca cctgtaacga tcaatccttc ttttgcgttc
      901 aaattcgttg cttttgctgc tttaaccatt tcactgcgca aatatgtgct tgcttcataa
      961 tatagaggca ttcctggtaa ttgtcctggt ttgtagccaa atcctgttgc atctgcatca
     1021 aaataagcta ctttgtctga tataacgatg tcacctactt gtaagccttc accgattccg
     1081 ccagctgaac ctgtattgat caccatgttc acgccgtatt gttggatcaa cagacttgtg
     1141 gtaatagatg ctaagacttt tcctataccg gaacgtacga cgatcacttc gtggttacct
     1201 aatgaacctg aaataaataa agcgcctgcg cgttcccatg ataatggttc gcttaggttc
     1261 tctctcaaaa tttttacttc ttcttccatt gcgccgataa taccgatttt catcttttta
     1321 attccttctt tctatataaa tgcgattgct aaaaaaacca cacataacat taggataaca
     1381 ataataagag atttcatcaa aaaagagttc catttggtcg tcttttcccg ttcgctgatc
     1441 cttgttttcg tgattggttt attctttttg ctttctttac gataaaagct ggcaatcttt
     1501 ttttcttctt gctgataagc tgtttctgct tttcgctgtt tttttaatga ctcgcttgct
     1561 tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt ggccataagc
     1621 cgatccctct actttccttt ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt
     1681 gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct cttcaagagt tagatgatac
     1741 aactccagta cttcgtcttc atcttgtgct aaaggattct cgaccttctc taccccttgt
     1801 gcatgataaa tatgaaggac ttcatttgca aaacctggtg aaagatacat cgaagtaaga
     1861 tggctcagcg acttcgcacg atagccagtt tcttcttcta attctctagc tgctgtcatt
     1921 tcgggatttt gtccttctcc tggatcgatt tttccagctg gaatctccaa aatcactttt
     1981 tctaaaggtt ttcgaaattg tttgacaaga agcagacgat cttgttcgtc aaaagcaatg
     2041 atcccgacac caccaggatg aaataccagt tctctcttcg aagttcctcc atctggcaaa
     2101 cgaacttcgt ctacagcaac atcaataatt tttccgctat agatttcttt gcgactgatt
     2161 gttttttctt caaattgctc attgttcagc atatcttctc ctgctttctt tgtaatcata
     2221 actgcttttt tttcattttt caatttttct caaaaactcc ttattagttt accatagata
     2281 aaccttagat tttctctaaa tatagaaaat ccttctttag tccgatactc tttctagtag
     2341 tccgtatgct aaaataagta tgtaatcgaa taacgaaaag aattcaatac tcaacgataa
     2401 atgacatgga gggtttatta tgataaaacg aaattggccg tggcttctag cattggtctt
     2461 gctatttctc tttgtccgaa acaatagttt ttcttttgtt ctaggacttt tattgatagg
     2521 tgtagtttta gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga
     2581 aattcctgca ctgactgacg aacttgaaac tcactatgaa aaaagcggta tgacagcgaa
     2641 tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca atcaattgca
     2701 aatgaatatg cagcagacag ccaaacttaa agcaatcgat ctccgccatg atccagtcaa
     2761 agcagcgaaa gctttattca aagaattggt gaaagaacca aaccgactgc atgaagcaag
     2821 tcagtttctt tatacgcatc tgccgaatct agttgatttg acaaataagt ataatgagat
     2881 caatgaccat gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat
     2941 catcgaccag ctgtcagctt taatcgcaca agattatcaa aaattcgttg ctgaggatct
     3001 agatgatatc gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg ataataaata
     3061 tgaaccagaa caaaaggatt aactcttttg ttcataaaag aagactcgga acattcagat
     3121 gatgtctggc tgtttcgagt actttttcta aagtagagac attcatgtaa gaatcgagaa
     3181 cagattcaac aaggaggaat tttttatgga tcataaaccg actgaaacaa aagatgttac
     3241 accagttaac gatacacttg aagatttatt gaacaacccg ttttcaactc caacagatac
     3301 gctgaccact tcacaacaag cggagattga tcagctccaa gagcaacaga cagctgctcg
     3361 tttaattgac aaactaccgg cggaacgcca agaacaagct aaacagctag cagcaaaaat
     3421 cgatgctaat gatgcacagt ctgtcatcag ctacggatcg gctgcccaag caaaattaag
     3481 tgaattctca caatcgatgt tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga
     3541 ttccttaact gaactgatgt atcgtctcca agaagccaat cctgacgaac tgcgtgccgg
     3601 agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac
     3661 agcaaaatat caaaagattg gtgcacaaat tgacaagatt tcagtgaaat tagataaaga
     3721 aaaagacggt ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga
     3781 ctactttgat gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca
     3841 aacaacgatc attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt
     3901 tcagattgcc aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct
     3961 tcgcttagct agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac
     4021 aaatcaggcc ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa
     4081 aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg
     4141 ccaagtctct gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc
     4201 agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac
     4261 cactcaaaat gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa
     4321 agaaaaacgt cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa
     4381 actgttggat ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa
     4441 cagtttatta caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga
     4501 gtctatttct cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca
     4561 ataaccatta cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca
     4621 atacaagtca agatggagac gattggcaat agcggaacga atggcacttt gaattctcca
     4681 gcttttggtt tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa
     4741 taggcgagcg ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca
     4801 aagaatacag ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga
     4861 tcgatttctg tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga
     4921 gcaagtgaat acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg
     4981 atcaccgcgc ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg
     5041 actcctagtt cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg
     5101 acagtagcaa tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg
     5161 acttcctcgg ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct
     5221 gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta
     5281 acgaaaaata ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg
     5341 gcattattca aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc
     5401 actacgagca ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt
     5461 aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat
     5521 ccggaagcaa cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc
     5581 caggcagcaa attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc
     5641 actcgcgaag caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag
     5701 acaaatgaaa gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca
     5761 aatattcccg taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt
     5821 tcttttttca tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt
     5881 gaagttcctc cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa
     5941 aggataccac ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc
     6001 gtttttcttt cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt
     6061 gaaagtgaga agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt
     6121 cctaagcccc ataataatcc acagatcatg tttttccaca caaactgatc gactttaact
     6181 ttacggaaag caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt
     6241 gacatcgcat ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta
     6301 gagaagatca atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt
     6361 tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca
     6421 ataataccgt atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca
     6481 atcgtattca aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg
     6541 tacttcattc catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat
     6601 ccgatcaaga aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa
     6661 aacagaaatg caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta
     6721 ccacttacta aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc
     6781 ataaccaatc tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca
     6841 caagcacttt ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat
     6901 tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat
     6961 acctaatttt tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt
     7021 gctaagtcga ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc
     7081 tgggataccg atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat
     7141 gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt
     7201 gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc
     7261 gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat
     7321 tccttttagt ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata
     7381 atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata
     7441 cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg
     7501 cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat
     7561 tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag
     7621 tttttcttct tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat
     7681 gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat
     7741 cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt
     7801 tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa
     7861 tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga
     7921 acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt
     7981 tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca
     8041 tcccacagtg ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa
     8101 ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata
     8161 cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct
     8221 gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg
     8281 aaatgttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc
     8341 gtattcctgc tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac
     8401 caataatagc catccaaaac gggaagatta atatctagta atacaagctg tggttcttct
     8461 ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat
     8521 ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt
     8581 tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg
     8641 tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa
     8701 tatgcgattt aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa
     8761 taattggtct attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa
     8821 caccaataac cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga
     8881 taaagatggt atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact
     8941 cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt
     9001 gtgactagat cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca
     9061 attttctcaa cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca
     9121 tcttgctcca tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg
     9181 gtaatcacgg aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc
     9241 gctcctctat ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt
     9301 ttcggaatat agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg
     9361 acatttctaa ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat
     9421 gtatgagcaa gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct
     9481 tctttatcgc gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg
     9541 ctgagagcat ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact
     9601 gccataaata attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt
     9661 ttcattttct tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct
     9721 gcagcttatt tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta
     9781 cgctccaatc atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt
     9841 agcaggaagt gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc
     9901 tacgatctga atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc
     9961 ctcacctgtt ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc
    10021 ataatctaac ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg
    10081 tccgcttgtt gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc
    10141 ccctttataa aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc
    10201 tttaagcaat tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac
    10261 tttctctggc ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat
    10321 aaccgctgtg ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata
    10381 cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc
    10441 tactccattt tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat
    10501 ataatcttgt ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata
    10561 ataagttaga ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc
    10621 gttttcaagg acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt
    10681 ttttcctctt tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat
    10741 cgttacttgt ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc
    10801 gtttttcgga tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca
    10861 cacgccatcc accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat
    10921 tctggaaata tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga
    10981 aggtgcttct tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt
    11041 ttgtttgtca atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg
    11101 aatccattca atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt
    11161 atctcgaatc aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg
    11221 aatattttct tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac
    11281 agatgagaaa ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc
    11341 ttcggctttg gctagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag
    11401 tacactcatt cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta
    11461 tt
//
LOCUS       DAZUKR010000081        11224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000081 DAZUKR010000000
VERSION     DAZUKR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.083"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACG89C_001909"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACG89C_001909"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4900441.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="ACG89C_001910"
     CDS             2714..2938
                     /locus_tag="ACG89C_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900442.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            2951..3454
                     /locus_tag="ACG89C_001911"
     CDS             2951..3454
                     /locus_tag="ACG89C_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4900443.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            3514..3903
                     /locus_tag="ACG89C_001912"
     CDS             3514..3903
                     /locus_tag="ACG89C_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4900444.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            3890..6337
                     /locus_tag="ACG89C_001913"
     CDS             3890..6337
                     /locus_tag="ACG89C_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4900445.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            6342..8465
                     /locus_tag="ACG89C_001914"
     CDS             6342..8465
                     /locus_tag="ACG89C_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4900446.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            8462..9466
                     /locus_tag="ACG89C_001915"
     CDS             8462..9466
                     /locus_tag="ACG89C_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4900447.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            9485..10396
                     /locus_tag="ACG89C_001916"
     CDS             9485..10396
                     /locus_tag="ACG89C_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4900448.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            10583..10839
                     /locus_tag="ACG89C_001917"
                     /pseudo
     CDS             10583..10839
                     /locus_tag="ACG89C_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3779 a         1846 c         2420 g         3179 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 ccaatcctta gtcttactgg caattgtggt cattggtttg tttttaggat ttaagcggga
     2821 gttttccaaa ctgattggct ttttggtgat tgctttgatt gcggtcggat tggtctttaa
     2881 tgcttctggg gtgaaggatg tcttactcag tttgtttaac cgaattgtcg gtgcgtaagg
     2941 gggaagcacg atggaacaga tgcgtgttta tattgcgaac ctcgggaaat ataacgaagg
     3001 tgaattggtg ggggcgtggt ttaccccacc tgtcgatttt gatgaagtga aagaacgaat
     3061 tgggttaaat gacgaatacg aggaatatgc aattcatgat tatgaacttc cctttgagat
     3121 tgatgagtat acgccaattg aggaaattaa tcggttgtgt ggcttagcgg aggaacttga
     3181 aggaacgcca gttggtgaag ttgcttctga gattcaacat gctttcttca attcttttga
     3241 ggagatggta gagcatgtgg atgatattat ctattatcca gattgcgatg atatgagtga
     3301 tgtagcatat ttcttgattg tagaagctgg tgattttgga gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtag agacttggag attggaggca attatttggt
     3421 aacgagtcga ggtgtttttg agtatccata ttaacaaaat tattgaaggg tagttgattt
     3481 ctaaactatc cttcttttta aaggaatgat ttgatgaaga aaataaaaag ctacaccagt
     3541 atttgggcag ttgaaaaggt catttatgca attaatgact ttcaactgcc ttttcctgtg
     3601 acttttaacc agatggcttg gtttgtttta tccttgctgt ttgtcatcgt atttgcccat
     3661 gtgcctccac tatctatgat agagggggcc tttttaaaat acttaggtat accggtggca
     3721 gttacttggt tcatgtcgca aaaaacgttt gacggtaaga aaccattggg atttttacga
     3781 tcttttatta gctatcactt acgatttaag gtcactttcg caggaaagaa agtcaaagaa
     3841 cagaagaaac gatgggacga acccattact ctggtaagga gtgtgaacta tgtacccgat
     3901 taagtatatt gaaaataatc ttgtctttaa tcaagaggga gaatgttttg cctactatga
     3961 gttaatacct tacaattact cctttttgtc acctgaacag aaatatcaag tccacgataa
     4021 ctttcgtcaa ttgattgcgc aaaatcgtga aggcaagatt catgccttac aaattgccac
     4081 agaaagtagt attcgtgcca cgcaagaacg atcaaaaaag gaaattaccg ggcgattaac
     4141 agaagtagcg aaacaacgaa ttgatttaca gacagaggca ttagtatcca tgattggcga
     4201 tagtcagatt gattatcgct tttttattgg cttcaaactg attgcgacag atgaagaggt
     4261 caatctaaag aatctcaaga aatctttctt ctccggcttt caagaatttg tctatggagt
     4321 gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc gccgatatac
     4381 caagcttgaa aaactaatgg aaagtaaatt ggctcgaaga tttaaagtca gacgagtcac
     4441 accgagtgat ctgatgtatt tgattgaaca tatctatggc gaaaagggta caccatttga
     4501 agaatatgaa tttcagctac caaagaaaaa gctgaagtcg gaaactttgg tgaaacgtta
     4561 cgacttatta cgtcccagtc gttgtttgat tgaagaaaag ccccggtact tatgcatgga
     4621 acatgagaat catgaatcat atgtagcgta cttaacaatt aatacaattg tgggggaaat
     4681 ggagtttccc tcctcagaac ttttctatta ccagcaacaa caatttacat tcccaattga
     4741 cactagtatg aatgtggaga ttgtgacgaa caaaaaggca cttgctaccg ttcgcaataa
     4801 gaaaaaggaa ttaaaagact tggacaacca tgcctatcaa tctgacaatg aaacaaattc
     4861 caatgtctta gatgcattgg attccgtaga tgaattggaa actaccttgg atcagtccaa
     4921 agaatccatg tacaaactga gctatgtggt tcgagtaagt gccgaatcag tagatgaact
     4981 aaagcgccgg tgtgatgaag tgctagattt ttatgatgat accaatgtaa aactagtccg
     5041 gccttttggc gatatgatgg ggctacatga agaattctta ccttctagta agcgatacat
     5101 gaatgattat atccagtatg ttacgtctga ttttctcgcc ggacttggtt ttggcgccac
     5161 tcagatgtta ggggagttgg aaggcattta ctttggttat aacgtagata cggggcgcaa
     5221 tgtttacctg aaaccggcat tggcctcaca aggggtcaag gattcagtta ccaatgcgct
     5281 agcagcggca ttcctcggtt cactcggtgg cggaaagtcc tttagtaaca atctcttagt
     5341 ttattacgcc gtcctttttg gtggacaagc tgtgattgtc gatccaaaag gggaacgggg
     5401 tggctggaaa gaaaccttgc cagaaattgc ggatgaaatc aatattctca acttaactag
     5461 taagccaaaa aatcaaggat tacttgatcc ttatgtgatt atggagaaaa agaaggattc
     5521 tgaaagtcta gcaattgata ttctgacctt tttaacagga atttctagtc gagatgggga
     5581 gaagtttcca gtgttgcgta aggccattcg gacggttacc caaagcaatc aacggggatt
     5641 attacaagta attaatgagc ttcgaaaaga aggctcacca gttgcggaaa atatcgcgga
     5701 tcacatcgag tcctttaccg attatgattt tgcccatttg cttttctcag atggttcgat
     5761 tacccagtcg attagtttag ataggcagtt aaatatcatt caagtagcgg atttggtttt
     5821 accagatgca gaaaccactt ttgaagaata taccaccatg gaattgctta gtgtttccat
     5881 gctaatcgtc atttccactt ttgccttgga ctttatccat agtaatcgag aaatcttcaa
     5941 aattgtagac ttagatgagg cgtggagttt tcttcaagta gcgcaaggga aaacattatc
     6001 gaataagctc gttcgtgctg ggcgggctat gaatgccggg gtttactttg tcacgcaaaa
     6061 tagtgatgat ttactggatg aacgcttgaa gaataatatc gggttgaagt ttgcgtttcg
     6121 cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggcttgg atcgagaaga
     6181 tgaaagcaat caaaaacgcc tacgagattt ggaaaatggc caatgcttga tgcaggattt
     6241 gtatggtcgg gtaggcgtgg ttcaggtaca tccagtcttt gaagaactct tccatgcctt
     6301 tgatacccgt ccacctgttc aagagagaat ggagtgaggg gatgaaaaag aaactttttc
     6361 ggattgtggg aatcgttttt attagcatac tcactctgat agttctattg agtttagttg
     6421 gtacggtagc cgaggcgaca gggctagttg atgatacagt tgaagcagga aatctatatt
     6481 ccaaatattc actgaacaac tatcaactag atttctttgt ggatagctct tgggattggt
     6541 tgccttggaa ttggggtgat ggcttaggga aaagcgtgat gtatggtctt tatgccatta
     6601 ctaatttcat ttggacggtg agtttgtatt tgtcgaacgc cacgggttat gtggttcaag
     6661 aggcttacaa attagatttc atttcggata cggctgaaag tattggaaag aatatccaaa
     6721 cattggctgg tattactgaa aatggactcc agacctccgg attttatttt gggtttctat
     6781 tattgatgat tttagctctg ggggtgtatg tggcgtatac cggattatta aaacgagaaa
     6841 ccacgaaagc agttcgagcc gtgattaatt ttgtgatgat ttttctacta tcgggttcgt
     6901 tcattgctta tgcgcctacc tatatcacaa aaatcaatga ctttagctca gatgttagtg
     6961 aagcggccct tacccttgga accgagatcg ttgtacccaa ttcagaaagc caaggaaaag
     7021 atagtgtgga tttgattcgg gatagtttgt tttcgattca agtccaacaa ccttggttac
     7081 tactgcaatt tgatgattcc aacatagaag aaattggtga agaccgggtc aacaagattc
     7141 tatctgtgag tccagatgaa aacaaaggca aagatcggga agaagcggtc aaggcagaga
     7201 ttgaagacaa tgacaatgcg aatctcagta ttaccaagac catgaaccgt ttagggaccg
     7261 tggtcttttt ggtattgttc aatattggca tttccttctt tgtttttctt ctaacaggga
     7321 ttatgttgtt ctcgcaaatt ctcttcatta tctttgcgat gtttctgcca attagtttct
     7381 tattgtccat gttgccgact tatgaaagtt taggaaagaa ggcgattatt cgtttattca
     7441 atacgattat gatgcgagcg ggtgttactt tggtgattac gactgcattt agcatctcca
     7501 cgatgttttt caatatttcg gcaacctatc catttttcat ggttgctttt ctgcaaattg
     7561 tgacctttgc cggtatctat ttcaaattgg gcgacattat gagtatgttc aacctccaga
     7621 gtaatgatag ccagtcaatg ggaagacgcg tcatgcgaaa accgcaaatg ttaatgaatc
     7681 gaaaattgcg gcaactcaat cgaaatgtgg gacgagcttt ggcttttggt ggtggtgcag
     7741 cagtcggaaa taagttggca aaggaacaat caaaatccaa atttaagcca tccggttctt
     7801 ctctacgaaa gaattcacga ttaccgaacg atcacgaaat actatctgac ttaactaagg
     7861 agaatccagt ttcaaataat aagaaacaat cccgaatgaa tttggctggg agaaaaatcg
     7921 gcaaagtttt ggatacccaa gctttggtga aagataagac taaacaagta aaggatcagg
     7981 taaggaatac gccaacgaat ttgaaataca atctccataa aggaatcgaa aagtcgaaaa
     8041 aagcaccaga agaattcaag cgcggactgt tccaagaaaa agcggatcga gtggatttgc
     8101 gagaaaaaca acgacaacga agaaatgcga aaatggacga aaaacgcaag atactgagtg
     8161 aagcaacaaa tcgtcaggga aaaagaaggg cgaatattcc aatagataaa gcagctcaac
     8221 ctcagaaaga tatgatgctg aaaaaagaag caccaaagcg gattgtgaga gcaaatctga
     8281 atcccgaaat caaacgaaaa ctaactagtc aagaaatcat ttcaaaaggg aaaagccaat
     8341 cagcaagtaa gaaaaattct ttccaacaag tgaaacagag gtccactctg ccaaagcaga
     8401 ccaatcaaaa agtacaaaaa agaatgaacc atcgtccaaa atcaaggtca ggtgagaaaa
     8461 aatgaacttg aagaaagtgg gtgttatttc attaactttt ctgaccatct cttttatggg
     8521 tattttgatg tgtgtgggtt tgttgtttgg tgaagacagt gagggaaatg gtagttctgg
     8581 cacttctcct ggcggaacta gtgtctcaga agaagtattg aaacaccgtg caatggtgga
     8641 aaagtatgcc aaagaatcgg atataacaga gtatattcca attcttttgg cgattattca
     8701 agtggaatct ggtggcacaa tggaagacgt tatgcaaagt tctgagtcag ccggattacc
     8761 acctaattca ttaagtaccg aggcatccat caaacaaggc tgtctctatt ttgcgtcttt
     8821 ggttaagcgt tccaaagaac tagggtgtga tcaagatagt attattcagg cctataacta
     8881 tggtggcggg tatctagatc atgtcgggaa aaacgggaag aagcatagtt ttgctttagc
     8941 tgaatctttt tcaaaggaaa aatcaggtgg tcaaaaagtc gattacccca acccaatcgc
     9001 gattaaggaa aatggtgggt ggcggtataa ttatggcaat atgttttact gtttattagt
     9061 gaagcaatac ctaactacaa ctcagtttga tgacaaaata gtccaagaaa tttttgatga
     9121 ggcatttaag tatgaaggaa cggcctatgt gtttggtggc tcaagtccag aaacggggtt
     9181 tgattgtagt ggcttgaccc aatggtgtta tgccaaagtt gacctgaaac ttcctcgggt
     9241 ggctcaggat caatatgatg ccatgactca tattgatcta aaagatgcca aaccaggtga
     9301 tttagttttc tttcattcaa cctatgatgc cggtacttat gtgacccatg tgggcattta
     9361 cgcaggggag aatcgcatgt ttcatgcagg agatccagtg gggtggacga atctaacgga
     9421 aaactattgg cggcagcatc ttatcggagc tggccgatac aaataagaga aaagaggaaa
     9481 aagaatgaag ataaaaattg aacggaatca aaaggaaaag agcccaaaaa agaaaaaaga
     9541 acgagttgta tccgtgggta agcatcgaaa aatggtgctt gccctttggc ttttattgtc
     9601 ttgtagtctt gcttttggcg tttataaaaa tttcaccgcc attgatcaac atacaactca
     9661 tgaaaaagtg gttataaaag agaaggtagt gaatacttct ggcgttgaaa gttttacaga
     9721 ggattttgcg aaagaatatt tttcatggaa aaataacaaa gaagcaattg aaaagagaat
     9781 gagtaatctc gaacagtacc ttacagaaaa cggtctcgct ttaatccaag atatggttcg
     9841 agcagatatt cccacgagtt ccgaagttca atctgtaaaa attctagatg ttgaaaaaca
     9901 ctcggaagaa tttgtcgttt catttttagt ggagcaacat attacagagg gaaaaaagac
     9961 gcaatcaatt tcttctgcat atcgagtgac aatctttgaa gatgaaaaca ggaatcattt
    10021 agtaactagt ttgccaacaa tgattggaaa gccagacaag gcaaaatatg aagcaaaaca
    10081 agtagaaaac gattcagaaa ttgatgccaa aacaacggaa gaaatcaccg attttttaga
    10141 aacatttttt aaactttatc catcagcttc tgaaaaagaa ttggagtatt atgttgaaaa
    10201 tggtgcgatg cgaccaatca atattgactt aaagttttcg gagttagaga atccagttta
    10261 tctacaaaac ggaaaaaatt ttctagtgag tacaaagata aaataccttg ataatgtatc
    10321 aaaaaccact agctactttc agtacaaatt agtattacaa aaaaatggaa actggaaaat
    10381 cgttgatgtt aaataataat gactcaatct aattttatta gaagttataa gtagtgtaac
    10441 ttatttcagg cagaagtcgc ccatcctcta taggtgggtg atgaatgcca ttcggtaact
    10501 cgaaaaccga caattaagac atagtgcctt tttattttaa tccatgtcat aatcagtcta
    10561 tataggaaat ggttgatata ccatggaatt agatacaaat aatcattcag tttttcttct
    10621 tggttacccc cttattttag tagtgaaaca ttgtaaacat gtcattgatg atgtaatgtc
    10681 agcttatgca aaaactgcct ttgaaagaat ttcagaatca catcacatta ccttagatga
    10741 atgaaatcgt gataaagaat actttcatat tatgctcaaa gcacaaccta aaaccgaatt
    10801 aacaaaattc agcaatgcct aagctcacgt ctgataaaat gagactttct aagggtcaaa
    10861 aatttccttt gaatacgatt attgcttaga taaaagtaaa agccttagaa ctacggggat
    10921 agcttggtta ataagagata tcgttgttag taaagacata ggcttgcaag tatgctctat
    10981 tcccaagaat ctcccccttc aagcaccaag gatcgttaaa ttcagctaag tggcaagtaa
    11041 ttcactagac gataattatc tccatatttc aatctcagct tttccatttt tcatagcaaa
    11101 ttccgttatt tttatagcat ctaaaagatt cagcttttcc actgatcttt caccactttt
    11161 cagctttttt attgtgctta aattgatacc agtacctttt gatattgaat tggaagtaaa
    11221 atca
//
LOCUS       DAZUKR010000082        11189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000082 DAZUKR010000000
VERSION     DAZUKR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.084"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..1410
                     /gene="lysA"
                     /locus_tag="ACG89C_001918"
     CDS             151..1410
                     /gene="lysA"
                     /locus_tag="ACG89C_001918"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4900449.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1397..2197
                     /gene="dapF"
                     /locus_tag="ACG89C_001919"
     CDS             1397..2197
                     /gene="dapF"
                     /locus_tag="ACG89C_001919"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4900450.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2200..3081
                     /gene="dapA"
                     /locus_tag="ACG89C_001920"
     CDS             2200..3081
                     /gene="dapA"
                     /locus_tag="ACG89C_001920"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4900451.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3078..3794
                     /gene="dapB"
                     /locus_tag="ACG89C_001921"
     CDS             3078..3794
                     /gene="dapB"
                     /locus_tag="ACG89C_001921"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4900452.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3800..4495
                     /gene="dapD"
                     /locus_tag="ACG89C_001922"
     CDS             3800..4495
                     /gene="dapD"
                     /locus_tag="ACG89C_001922"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS4900453.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4511..5512
                     /locus_tag="ACG89C_001923"
     CDS             4511..5512
                     /locus_tag="ACG89C_001923"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4900454.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5509..6870
                     /locus_tag="ACG89C_001924"
     CDS             5509..6870
                     /locus_tag="ACG89C_001924"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS4900455.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6830..7981
                     /locus_tag="ACG89C_001925"
     CDS             6830..7981
                     /locus_tag="ACG89C_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4900456.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7956..9125
                     /locus_tag="ACG89C_001926"
     CDS             7956..9125
                     /locus_tag="ACG89C_001926"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4900457.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9300..10679
                     /locus_tag="ACG89C_001927"
     CDS             9300..10679
                     /locus_tag="ACG89C_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4900458.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10960..11189
                     /locus_tag="ACG89C_001928"
                     /pseudo
     CDS             10960..11189
                     /locus_tag="ACG89C_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3728 a         1953 c         2498 g         3010 t
ORIGIN      
        1 aaccattttt attcttattt ttaaaaaaat atttcgaaat atactagaca attcagaaaa
       61 ttttgtttaa tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa
      121 cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca
      181 gcaagcacat tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg
      241 agacaaacga tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg
      301 ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga
      361 ttcgggctag atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct
      421 agagatacta tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt
      481 gaaaatgatt tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa
      541 ttaagccaac gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa
      601 gcacatactc atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat
      661 gaaagtaaag agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta
      721 gaaggatttc atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa
      781 atcgacaaat tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc
      841 ggagggtttg ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta
      901 aaaaaactga ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa
      961 ttactgatcg aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt
     1021 ggtttcatca aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat
     1081 aatatccgtc cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg
     1141 gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta
     1201 attaaagaag cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg
     1261 ggagcttatg gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt
     1321 tttgtcaata atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta
     1381 aggggggaag tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt
     1441 attagattac gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga
     1501 aaaatatgat acagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta
     1561 caacaaagat ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata
     1621 cgtaaatgat cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac
     1681 aagaaaaatt acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc
     1741 agatgattct ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat
     1801 agagatcaat ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcatacagt
     1861 tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca
     1921 tcatcctttg ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga
     1981 attgatcgtc cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg
     2041 ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt
     2101 tcacttggaa caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc
     2161 agcagttcat gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg
     2221 gcgctgatca cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg
     2281 atccaattcc atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa
     2341 tcttctacgc tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg
     2401 gctggccgtg tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa
     2461 aaagcgaagc attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac
     2521 aacaaaacaa gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg
     2581 attccgctta ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta
     2641 ttagtaaatt tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg
     2701 gcttatgtga tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat
     2761 gatgatttga tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg
     2821 aatatccaac ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag
     2881 caagcaaaag aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc
     2941 aatcctattc ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc
     3001 ctgcctttag tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa
     3061 gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag
     3121 tcgaagaagt tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag
     3181 aagcaactac gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag
     3241 ataatttaga gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca
     3301 cgacaggtta tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg
     3361 tgatgtatag tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag
     3421 cagctgcaat gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa
     3481 aagatgcacc atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc
     3541 tgcagcccgt atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca
     3601 gtattcgtgc aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa
     3661 tatttacgat caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag
     3721 cagcggactg gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg
     3781 acaaaggggt gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc
     3841 taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag
     3901 taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc
     3961 ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata
     4021 cagcgcagtt cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat
     4081 tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa
     4141 tattggtgca gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg
     4201 agcgacagtg ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga
     4261 accagcatcg gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt
     4321 gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt
     4381 agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag
     4441 tgatgatcag acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag
     4501 atggagaaaa atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca
     4561 aacgatgctc aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc
     4621 ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga
     4681 attgacagaa acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt
     4741 atccaaagag tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc
     4801 agcatggcgg gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta
     4861 tacattgaat cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct
     4921 taaagcttta caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt
     4981 ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc
     5041 agttttgtat ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga
     5101 aaatgggtat acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca
     5161 gcctgctttg ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt
     5221 ctctgtggca gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag
     5281 ccagtttcca gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat
     5341 tcttgctaga ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga
     5401 gaacggattg ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc
     5461 cgtacagatt gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg
     5521 ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc
     5581 cgtacaattg ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa
     5641 cgtacgtcag atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag
     5701 catcatgttg attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga
     5761 gatgttcttg ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg
     5821 gaagacgaaa agataactga agattattta gtaagtagag gtgaatatct gactgctcaa
     5881 ctgatggctg agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac
     5941 tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga
     6001 tatccgaaaa tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta
     6061 ttgggccgtg ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa
     6121 agttatgaga attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat
     6181 catcctaaaa tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg
     6241 gctaacgttc ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa
     6301 ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa
     6361 aaggaaaatg gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc
     6421 aaacgtcata tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa
     6481 catgagatat caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca
     6541 gcagaagcga ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc
     6601 gtggaagaaa tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat
     6661 aaagaattga ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc
     6721 acatcgatct tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat
     6781 caatatgaaa cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga
     6841 atatcgaatg gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa
     6901 atatttatat gaacaagtaa aaaacagcgg ggccgttatc catgaaatcg gcgaaacagg
     6961 actactcctt tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga
     7021 tgcactgcca gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat
     7081 gcatgcatgt ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac
     7141 taaacacatt gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat
     7201 ttccggtggc gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc
     7261 aatatttggc tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg
     7321 acctttgatg gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat
     7381 tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt
     7441 ttatgatttt gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat
     7501 ccaaggtgga acgatccgca atgttttagc tggagaagtt attgtatccg gaagtatccg
     7561 ttcttacagt tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca
     7621 gtttcactct acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa
     7681 ccatagaggc ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc
     7741 agttcttcaa gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt
     7801 tttaggtgtc ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt
     7861 tttagaaaat ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa
     7921 aatcgatagc agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta
     7981 gtcctaggct ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt
     8041 cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg
     8101 tcaagcaagc aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag
     8161 gattagagga actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt
     8221 atgatccgca aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt
     8281 tgttatctgt tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat
     8341 atcagccatt agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag
     8401 ggtttgtctg tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca
     8461 aagcagtcat tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat
     8521 tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat
     8581 atgctgaatt gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa
     8641 caatcgtgat aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta
     8701 tttttgctca aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg
     8761 ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata
     8821 gcgagaatat gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc
     8881 ctttaggatt tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag
     8941 ctgccattca aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg
     9001 cgtgtatccc aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg
     9061 ctagcagcat ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac
     9121 agtgattcat agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga
     9181 gacgattgtt ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt
     9241 aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa
     9301 tggaaaaaat caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg
     9361 aactattgac acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata
     9421 tttccttcat gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg
     9481 atgtacgtaa tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc
     9541 acgaagtaaa aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata
     9601 acgaaacaaa agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca
     9661 atctgccagt accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg
     9721 caaccaaaat caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg
     9781 cgggttacat tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat
     9841 tgcttgatgt gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt
     9901 tagaacaaca cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt
     9961 ttactggtaa cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc
    10021 tagtcattca agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact
    10081 tagatgaacg tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt
    10141 atgcagtagg agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg
    10201 ctttagcgac tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag
    10261 taccaaacaa accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc
    10321 attttgcagc aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag
    10381 tgtcttatta tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa
    10441 aagtgtccat ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat
    10501 tatctaccta tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac
    10561 tgactttaga agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat
    10621 ggagcatgtt gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag
    10681 aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt aataagaaca
    10741 gccaagtaaa aacagcttct tatgttttta acttggctgg acttattacc gcagatgcaa
    10801 gcttttgaac accgtttatt ttcaaaaaag ggactgtgac acgatttgtg taccgacccc
    10861 caaaagttag accaaaaaat ctaacttttg gaggtcggta ttttttatgg ttaaatatac
    10921 ttttgaactt aaattaaaga tcgttcatga ttatttggat ggcaaaggcg attggaccgt
    10981 aaaaatccag atcaagagat tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat
    11041 tatggctgtt tacggatgac caatgaacta agaaaccgcg gtttctctat caataaaaag
    11101 aaagttcagc gcttgatcaa gaagctagga ataaaagtaa cggcatacac gcgtaaatca
    11161 cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZUKR010000083        11099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000083 DAZUKR010000000
VERSION     DAZUKR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.085"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG89C_001929"
     CDS             136..309
                     /locus_tag="ACG89C_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900459.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACG89C_001930"
     CDS             949..1422
                     /locus_tag="ACG89C_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4900460.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACG89C_001931"
     CDS             1431..1658
                     /locus_tag="ACG89C_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900461.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACG89C_001932"
     CDS             2294..2665
                     /locus_tag="ACG89C_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900462.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACG89C_001933"
     CDS             complement(2921..3163)
                     /locus_tag="ACG89C_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4900463.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACG89C_001934"
     CDS             complement(3195..4097)
                     /locus_tag="ACG89C_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4900464.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACG89C_001935"
     CDS             complement(4110..4298)
                     /locus_tag="ACG89C_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4900465.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG89C_001936"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG89C_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4900466.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACG89C_001937"
     CDS             complement(4913..5806)
                     /locus_tag="ACG89C_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4900467.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACG89C_001938"
     CDS             complement(5884..6807)
                     /locus_tag="ACG89C_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4900468.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACG89C_001939"
     CDS             complement(6856..7206)
                     /locus_tag="ACG89C_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4900469.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACG89C_001940"
     CDS             complement(7239..8141)
                     /locus_tag="ACG89C_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4900470.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACG89C_001941"
     CDS             complement(8134..8991)
                     /locus_tag="ACG89C_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4900471.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ACG89C_001942"
     CDS             9325..10134
                     /locus_tag="ACG89C_001942"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4900472.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ACG89C_001943"
     CDS             10174..10671
                     /locus_tag="ACG89C_001943"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS4900473.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3245 a         2077 c         1866 g         3911 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagcc
//
LOCUS       DAZUKR010000084        11043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000084 DAZUKR010000000
VERSION     DAZUKR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.086"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1061
                     /locus_tag="ACG89C_001944"
     CDS             <1..1061
                     /locus_tag="ACG89C_001944"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFS4900474.1"
                     /translation="VTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSVTSLSRLEF
                     IKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKELLTQIDREF
                     YFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEIGSTIEQSG
                     PLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQIYLGRYFK
                     MFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYIKRGFGLGI
                     QLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYIKELGSVEY
                     KKIANFESLYKLFNETKL"
     gene            1105..2277
                     /locus_tag="ACG89C_001945"
     CDS             1105..2277
                     /locus_tag="ACG89C_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4900475.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            2264..2968
                     /locus_tag="ACG89C_001946"
     CDS             2264..2968
                     /locus_tag="ACG89C_001946"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4900476.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2973..4133
                     /locus_tag="ACG89C_001947"
     CDS             2973..4133
                     /locus_tag="ACG89C_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4900477.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            4209..4487
                     /locus_tag="ACG89C_001948"
     CDS             4209..4487
                     /locus_tag="ACG89C_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFS4900478.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            4468..4839
                     /locus_tag="ACG89C_001949"
     CDS             4468..4839
                     /locus_tag="ACG89C_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFS4900479.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4855..5259
                     /locus_tag="ACG89C_001950"
     CDS             4855..5259
                     /locus_tag="ACG89C_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900480.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            5256..5666
                     /locus_tag="ACG89C_001951"
     CDS             5256..5666
                     /locus_tag="ACG89C_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900481.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            5727..6314
                     /locus_tag="ACG89C_001952"
     CDS             5727..6314
                     /locus_tag="ACG89C_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS4900482.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            6387..6854
                     /locus_tag="ACG89C_001953"
     CDS             6387..6854
                     /locus_tag="ACG89C_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFS4900483.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6900..7208
                     /locus_tag="ACG89C_001954"
     CDS             6900..7208
                     /locus_tag="ACG89C_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900484.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            7403..>11043
                     /locus_tag="ACG89C_001955"
     CDS             7403..>11043
                     /locus_tag="ACG89C_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4900485.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSE"
BASE COUNT         3982 a         1667 c         2409 g         2985 t
ORIGIN      
        1 ttgttacaat atttataata atagcaattt gtgttttgac agtattttta ccacaaatta
       61 ctgagtattt aaattatcgg atatttacca caaaagtaga tgatagcgtt actagtttaa
      121 gtaggctaga atttataaaa atatgtgcat ctgtgtttgg tcctattttg actgtaatgg
      181 tatttgtgaa tactttaaat atgcagaaaa aaacagagca aaagcaaaat gagcttgaag
      241 taaagcaagc taaggctgag aacgagagag caaaaaaaga gctgttaaca cagatagatc
      301 gtgagttcta ttttttatta aatatgttta ttgaaataca aaaagatcaa aatgccaaac
      361 aagctgttat tgctttacag aagttggcaa tttttaacga aaagggtttt aatcaagact
      421 atgcatttac ttctaaagat ttatcagata gtgaaattgg gagtacaatt gaacaatcag
      481 gaccgctgaa gggcacagtt gaatatataa aatttgaaac atttgatgta agcaagtcga
      541 tggggaatta tcaaacaaaa acaaaagacg ttgctacaag tttacacgat cagaaagtag
      601 ctatattgaa tgaacaatat gaaaagatgc aaatatatct tggacggtac ttcaaaatgt
      661 ttcaccgaat agttaaaaca ttaaatgagt attatgacga ttataatgat tttgatgtga
      721 agaggtatac aaaatatata gggacattaa gaacacaaat ttctccagct gaattccaag
      781 tgattttatt caattctcta tatataaaac gaggttttgg cttaggaatt caacttattg
      841 gaagtggttt ttttggagat gattttgatt ttgaaactaa tcaacatttt gaaacaagta
      901 tcaatgaaca gtggtttcta tcactatcaa ctgttgacag tgataactct ataaagcgac
      961 aaaaattatc tgaatatatt aaagaattag gatccgtaga atataaaaaa atagctaatt
     1021 ttgaaagttt atataaactc tttaatgaaa ctaagctcta gaaatagggc ttttttattt
     1081 ttctttgaaa ggtggtgaaa acatgtgagc ttatttgata tctttaagca gtcaataaga
     1141 aatgaagagc cttctgagtg gataccggat tttgtatatg gagaggaagt atctgctcgg
     1201 gcatacctca aaattatggc aaaaaatact gttttagatt ttgttgcaag aactatgtca
     1261 acgttagaag ttaagtttaa aaataaagat gggacatcag actgggatta tattctaaat
     1321 gttcgaccta attctgatat gtcagcagct acattttggc aaaaattttt ttatcggctc
     1381 ttagatgaca acgaagtgtt agtaatattt acagatgata atcaattgct gattgctgat
     1441 gattttagtc gaacggagta tgccgtgtat gatgatgttt ttacaaatgt gatggtaaaa
     1501 aattttgtct ttgaacgttc atggaacatg tctgacgtca tttatatgga atataataac
     1561 gaagaattag aaaggtttac acgaggttta tttgaagatt acgccgcgtt gttcggacgg
     1621 attctagaag ttgcaatgcg taacaaccaa attcgtgggt ctgtatccat tgattcaacg
     1681 ggaacggcga atgatgaaaa aggaaaagat ggtaagacta gatctcaaag attacaagaa
     1741 tatattgata ggatttggaa ctcttttaaa actaactcag ttgcaattgt tcccaaagtg
     1801 aaaggctttg agtatgagga atacaccaat aaacaaggcg tatcgaatca atcgctagat
     1861 gaactgaata agatgaagtc gtctttaatt gatgatgttg ccaacgccat aggagtacct
     1921 acggcgctta tttatggtga aaaagctgaa ctggattcaa atattaaagc ttttcgtaaa
     1981 ttgtgtattg ctccactagt taaaaaactt caagacgaat tggtggcaaa aataattagt
     2041 cgtcaagagt acaaaaacgg ggaacgtata gaagttaaaa aagttctcca aaaagatcta
     2101 ttagagtatg ctactcaaat tgataaaata acatctggtg gtgcgtttta tattgacgaa
     2161 gttcgtgatg aagttgatta tgatgagttg ccaaataatg aaggaaaaat tcgtattcgt
     2221 acgaaaaact atgaagaaac tgtgaaggga ggtgagaacg acaatgccga aagctaaaaa
     2281 agtaccattt cagtttacta acgaaattca gaatggtaag cacattctca ccttgagtgg
     2341 aaatgtacag aaaaaatatt ggcgtgatga tgatgtcata aacgctaaag atattcgtga
     2401 ctctctagat actgtaaccg atgatattgt aatcaagttg aacagcccag gcggtgatgt
     2461 gtttgaaggg attgagattt acaattattt aaaagatcac ccatcaaata ttactgtaga
     2521 agttacaggg cttgccgcaa gcgcagcaac gtttattgtc gctggtgcca atgatgtgat
     2581 tatgaatgtt gggacttctt tgatgattca tgaagcttct acatttgctt gggggaataa
     2641 acaggacatc caaaaaacgt taaacgcatt ggaaacgatc gatgattcta tcttagctat
     2701 ttattcagat aagactggcc aatctgttga taagttgcgt gaatggatga atgaagaaaa
     2761 atggtttaca gcagatgaag ctgtcaaata tggatttgcc aattctgtga agcgtgaaga
     2821 accacaaaat gaacctcaaa atattgcagc gatgattcaa gatgctgttg ccgttgcaat
     2881 agcgaattta aaggaacctg ttactaataa aatcgaacaa gaatcaaaac aaaaatcatt
     2941 aatcgcacga ttgcgaaaag gagaataaag atatgttaaa aattacagat aaaactgcag
     3001 atgcaaaaaa ggcatttaat gctatttctt cgaaagaaga tgcaacaccc gaacaaatta
     3061 atgctgctct agaagaatat gtaaccgcca ttgcagaaga tgccggcaaa caggtgcgtg
     3121 ctgaatatga agaattgaaa aatgtgacgg acaaccgtgt attagaggct cgtggaattc
     3181 ctacactaac agcagaagaa acaaagtttt ataatgaagc cgtaaaatct ggaggtttcg
     3241 attctgacgt agtttggcca gaaacgattt tggaacgtgt ttttgaaaat cttcaaaacg
     3301 atcatcctat tttaggcctc attaacttta caccaactgt aggtcgtgtc aaagtaatta
     3361 gagcacgtcg taaaggtgtt gctgtttttg gaccattgca taaagacctt gaaggccaac
     3421 tagatgctga atttggtgca actgaatttg ttcaacttgc gttgaccgca tttttcttaa
     3481 tttctaatga taccttagat ttaggaccac gatggattga ccgttttatc aatctttctt
     3541 taagtgaagc agtacgtgat gtttgggcta aaaaaattat tacaggttct ggtaaagatg
     3601 agccaattgg attgcttaaa gatctagatg gtgcggtcac aagtggtgta tatccagata
     3661 aagctagcgc agggacatta acttttgcga aagagaagat tgttcttgaa cttgcagggg
     3721 tgatgaaaaa attatctaaa tacacataca aagtagatgg tgacgatgaa ggtgaagtga
     3781 aataccgttc tgttgctgga cgagtgtatc taattgttaa tcctgttgat tactgggata
     3841 tcgtagctag agttacattt gcaaatttaa acaatgtgta tgggactacg atgccattta
     3901 tcgctgttga tcatatcatc gaatctgtag acgtaccaga aagtaaatgt attgcatacg
     3961 ttgataaaga gtatgaagct actcaatcaa gagctgaaaa agtctatgta tataaagaaa
     4021 cttttgcaat gaaacgcgca actttgtatg ctgtcgacat gcttggtaat ggctatccaa
     4081 caaataatga tgcagcacaa gtatatgact tggactttac agcgggggag taatgtcccc
     4141 tgcaattggt gagataacac cgacgactga tggggcagtc attaatcttt cgtaaatggg
     4201 gttagagtat gaacactgaa gaatttataa aagaatataa ggcccgattt cgtattttcc
     4261 atacttccga agatgaggat atcgggaaac agttagagag cgggtttgcc gatataaaat
     4321 ctctaatcgg cagttttgat ccttccactt atgagaaagg aaaagaatta gtttatgaac
     4381 gtactcggta tctacggaac gaatcactag agtactttta cgacaatttt caaacaatga
     4441 ttatggatgc atcaattgat ctggcaggtg ctggatatgc caattaaatc aaattataag
     4501 aagcctaaaa ttgtagctgg tgatctaaat acgccagtta ctttttttga gtttcaacct
     4561 aataagggtc cagaacctgg tgatgaagaa aaaaaggaac tgtatttttg tacagcacta
     4621 gtttacaacc catcaatgaa agatcgtgac attcttggca caaaagaagg cgttacgata
     4681 aaaatacggg atccccatca atcttatatt cctactaaca aacataaagt aaggatatta
     4741 gattatcgat atggagataa agtttgggaa ataatcgatg tctcaccgga tttggaagaa
     4801 aattcaattg taaagatagt attagaatta attacttaaa tttggtgata aatgatgagt
     4861 aacgtaaagt ttgaaggcgt tcaagaaaca atccaagctt tagaaaaaaa gtatggagaa
     4921 aagaaaacta agacattaac aaaaaaagca atcaatgttg gcgctgaaaa agtagaaaaa
     4981 cagttgcaaa cggacatgac cgtttttaaa gacaaaggtt atacgatcgc tgaggttgtt
     5041 cgcaagaacg ctacgtatag agattacaag gcagaagctg aaatcggttg gaatggtcca
     5101 catcaacgat atagaatcat tcatttgaat gaatggggat atacacgaaa gggcaaacaa
     5161 attagaccga gaggatttgg tgtaataaca aaatctctta agaattccga gctgttgtac
     5221 tttgatgctg taggagagga ggttaaaaag agtttatgaa agatatgctt aatatcattt
     5281 acgaggctct ttgtttgaat gagtatattc attcaatgac ctacaacgag aataccgagc
     5341 aatatcgtat taaattttat gagcaacctg aaaccgccga taaaacaggt ccaatgatca
     5401 caattcgtcc tgtagataca cctaacgaag cgtaccacgg aagtaacaaa gagctttctg
     5461 tggagcatat gatacagatt gacgttgaat cgaaatatag agcaacatgc aagcagattc
     5521 aatatgagat aaaaaaagag atgaaaaaac taggctttgg ccaaatttcc ggtcaagggt
     5581 tagatgaata ctttactgaa acaaaccgct ttgtagacgc gcgtcgttac gaaggaaata
     5641 caaaaattta cgataccgaa tattagaaat taagacacga aaataacgtg tcttttttta
     5701 ttacccaaaa ttaggaggaa aattttatga ctttagtagg attcaaaaaa atgacaatcg
     5761 gggtctttga cagcacaggg aagattccag cagcaaattt atatgtaatt gaaggtgaac
     5821 aaaacaaagg ggcgactgta tctgctgaaa tcagtgggct atcaaaagaa ccatcaaaag
     5881 tttacggatc gaacatcgct tactatgtct ctcaaaaagg aacaggcgat gtttcagcta
     5941 cttttggatt gttagattta ccaacagaag taaatgacaa aattcttggt tacaaaaccg
     6001 atacaaacaa aatcagcttc ttgggagaag atactgagcc accatattgt gcaattttga
     6061 tggaatcgga ggatttgaac ggcgatacag caatgcttgc gatgttcaaa ggaaaattca
     6121 gtcgtgaatc tattaatctt aacactacga caaatgaagc tttcgaacca gaagcagaag
     6181 aatatgtatt ttcagcgatt gccaatgatg ttgaaggtga tgctaaaggc caaacagtcg
     6241 caaaatatgt tggagacgaa gaagcttcta ttactggttt gaaagagatg gtatttcctg
     6301 cagcggggga gtaactagcc ccgttgttgg agcaatcact cccactatta atggggcaac
     6361 agttgaatta agttaggagg ataactatgc cagatacatt taaaatctat aaaaaaaatg
     6421 gtactgaatt tacaaaagtc gcagagggtg aaagtccact ttctattact ggaattgcag
     6481 caaatactca agttgctaaa ggagattatc aagctacgcg tgtggtcgga ggtcaagaat
     6541 cagtaaaagt agatattcct gcattcacca ctcttccaat ttcagttact ggtgtgacgt
     6601 tggataaaac gacagctgaa gttgaacaag gcggaacctt aaaattaact cctactgtaa
     6661 caccagcaaa tgcaacagac aaaacaggaa gctggagcag ttcgaataca gctgtagcta
     6721 cggttactgg aggcaacgtt acagtaaaaa cagacgctga agttggcgga actacagaag
     6781 tttcatttac tactacggat ggcggtaaag tagcaaaatg tactattaca gttattgaaa
     6841 aagcagcggg ctagtcttcg gactagcttt tttatttgga aaataatgga ggaatctaaa
     6901 tggcaaaagt aagaattgaa ttgaaagaag aatcaggaaa gaaagtctat gaaaacatag
     6961 acacaacagg gaaagattat cgaaaagctt tagaaacaat caaaaagcta aatgaagacg
     7021 gagtaatgat ttggaatcgt ttagacattt atttggattt tgctgtaggt atcttcaggg
     7081 atagcaaatt gacagctgaa caaattcttg atggcttacc atctgagaag gtaatgagca
     7141 cattggacga catcctagga gaagtaatgg ggattgaaag taatccagac ccagacgcaa
     7201 aaaagtaaca cccgaagaag cagaagaaat gtacttaaac ctatgcagag agttagtgaa
     7261 agcgggatgg tcactatctg acatagaaga taattccttt gaaatgatga tgaaaattgc
     7321 gtgcacttcg cctaagaaag agaaacaaaa agaagttgat cttaaagact tcttgaaatc
     7381 catgtagaga ggaggtaaac atatggcaaa tggaaaacct ttaggcaata tgattatcaa
     7441 actagactta gacagttcag ctttttcaaa aggtctagcc ggagctaaaa acgctgttaa
     7501 ccatcaaatg aaagcaatga aatcacagat gcaagtaatg aactcttcag gaaattctct
     7561 cggtgcttta caagcaaaat acaatggttt aggcggagtt ctacaagcaa atgaaaagca
     7621 agtagaattg ctcactaagg cttataagga tagttttgac tctaacggaa acgctactgc
     7681 ttcgactgct aaatacgcta atcaattgaa tcaagcaaca gctagatcag caagttacga
     7741 atctcagatg aaaacaactg ttggacagat tgctcggatg aaagtggaaa cggaagggtt
     7801 tacggggaaa ctaaaggcta attctgatgt attgattaat tctgggaaaa aaattgaagc
     7861 ctttggtaaa ggagtttctg ggataggatc tgccctaact gttggagtaa cagctccaat
     7921 agtggcaggc gcaactgctg ttactagtgc agctatttca tgggaaagtg cttttgcagg
     7981 tgtaaaaaag acaaatgatg aagttgtaga ctcgaatgga aacgtagttt attcctatag
     8041 cgacttagag aaaggtctgc gtaatcttgc cacccaatta cctggtagcc atcaagaaat
     8101 agcaaatgta gccgaagcag ccgggcaatt aggtataaag actgaaaacg tagttagctt
     8161 cactaaaacg atgatagaca tgggtgaatc gactaatatg tcggctgaaa ctgcggcaac
     8221 ttctttagca cgatttgcta atattaccca aatgtctcaa aaagactttg ataaattagg
     8281 atcagtcatt gttgatctag gtaacaattt tgctacaacc gaatcagaaa tcactgaaat
     8341 gggattgcgg ttagcgggag ctggtaaaca gattggaatg agccaaggcg aaattctagg
     8401 atttgctact gctctatctt ctgttggtgt cgaagctgaa gcgggtgggt ctgccttttc
     8461 gaaggtcatg atccagatgc aactagctgt agaaaaagga tttggcgctt ttgatcagct
     8521 gaaacagatg gcagaagaac aaggtgttcc gtgggttaat ttagtaaatg ctgtgagaga
     8581 cggtggaaaa tcgttaaaag cagtatcaga gcaaatggga ttcacttcat ctgatttaaa
     8641 gaaaatgtat aaagaagcgg acaattcgaa aagttcgctt gaaaattttg cgaatgtggc
     8701 aggtgttaca agtgaacaat tccagaagtt gttcaagagt gatccttcag aagcaatcat
     8761 caagttcatt caagggctga aagattcaga aaaacatggt acttcagcaa tcaaagttct
     8821 ggatgacatg gatataaaag aagttcgact acgcgacagt ttattgagag ctgcaaatgc
     8881 tagtgacata ttcaaaggag caattgaaaa aggaaacggc gcgtggaaac aaaatacagc
     8941 tttaacaaat gaagctaata aaagatacga aacaactgaa tcaaagctaa agatattgaa
     9001 aaacgaagtg attgatacag caatcgatct tggaggtcca tttgtagacg cgttgcgtga
     9061 tggtctaaat gtatcgaaac cattgataaa aaacttaggg aatatggcga aagctttctc
     9121 agctatgagt gatgatcaac aaagaaacat tatcaaatgg gttggattag cagcagcagc
     9181 tggtccagcg ctgaagcttt taggaactgg tacttcagta ataggtaaag ttacttcaac
     9241 tactggaaga ttgacgaaaa gccttgttga tttagcagcg aaagcagcag aaaagaaggc
     9301 tatggatgcc tttgcatcgt cggtaacaac agctggtgct gcagctgcaa agacagcagg
     9361 agctggaggt attggcgggt tagtccctat gatgggaaaa acagctagtg ctgcaagtac
     9421 tgctgcgggc gccagcggaa taggcgcaat gacaacgtca ctaggtttgt taggtcctgc
     9481 tctacttggt attgttggcg ttggtggcgc actggcagtt ggctatggag catggaaatt
     9541 atttggtgaa gaagcttgga attcctcgca aagagtaaag caatggggaa cagatgtcgg
     9601 tgaggagata gatagtactc ttgacggagt acagaagaat attgaaggtg caaatggtca
     9661 attcggtctt ctcaaagagg gttttactgt tgatgatgca aataatatgg cggctaattt
     9721 cgcaaccgct ggaacatctt tggaaacatc tttaacgggt agaataaagg cattagacga
     9781 tgcactaaaa gggctacctc aatcagttca ggatgccatg aaagaagtag ctgaatcaga
     9841 agaagaaacc ttagggcaat ctctttctaa aataaaagac aacaatgaag aaataaaaaa
     9901 aatcagagaa agagccgcaa atcagaacag agacttgact gtttcagaac tacaacagat
     9961 acaggatcta atgaaaggaa cttctcaagc gtatgtggat acgctcgatg tttctgctaa
    10021 agacagaaaa aagatactat cagccatgaa tggtgatgtt gcacaagcta cagaagaaga
    10081 agcgaagacg tggttacaat ctttagcgaa acaaaaagct gcaactaccg aaaattatag
    10141 acaaatgctt gaagaaaaaa aggcggcttt aaaggaaaag ggagtaagca aagaattcat
    10201 tgatgcatta gaagatgaaa tgaatgatta catcaaaact acaaatcaag gattcgatag
    10261 ccaaatagct gctataacaa ataaatatcc acaattgatt aacgaagtga gtttatcaaa
    10321 tggtcaatta tttagcaaaa cggcagctgc tatggacaat actggtcagt atacaaaagt
    10381 ttttctcgaa agtaatgaaa agattttagc ccaagcgaag aaaacatcgg atgaaatagc
    10441 agataatgcc gaaaaaaatg cagaacaact tggtatggta gctgatgagg ctacgcatgc
    10501 tggatcaact tggaatggct tagttttaga cgaaaaaaca ggaaaagtaa aaactaatgt
    10561 acaagaagta gtgaatgaag caacgaagga tttcactaaa tggaatgaat tgaaacctgt
    10621 tgttcatgat gcgaaactaa caacgaatgc aaaagatgtc atttccatag cggcagtaca
    10681 aaatggctat tgggatgata tggaatggaa agaaaaagaa gcattattag aagatgagtg
    10741 ttctaaaaat gtatacaagg ctttagaaaa tgctggtaaa tggaatgagt tagatattcc
    10801 ttctaaagaa gcaatcctaa cttcaaatac tcctgaagtt atgggtgaaa ctctcatgaa
    10861 tttgggatta tgggatactt acaaacttca agtgaaagat ttagatttga atgcgtataa
    10921 agtatacgat gctatcgatg gatcagaaga aaaattaaat agttgggctc aattagataa
    10981 ccccacaaaa gaattgttat tagataactc agatttttct acaaaacttt ttaattccga
    11041 aga
//
LOCUS       DAZUKR010000085        10922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000085 DAZUKR010000000
VERSION     DAZUKR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.087"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG89C_001956"
     CDS             complement(<1..281)
                     /locus_tag="ACG89C_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900486.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACG89C_001957"
     CDS             453..1541
                     /locus_tag="ACG89C_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4900487.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACG89C_001958"
     CDS             1669..3045
                     /locus_tag="ACG89C_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4900488.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACG89C_001959"
     CDS             3446..5026
                     /locus_tag="ACG89C_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4900489.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACG89C_001960"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACG89C_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACG89C_001961"
     CDS             complement(5652..5972)
                     /locus_tag="ACG89C_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4900490.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACG89C_001962"
     CDS             complement(6120..8354)
                     /locus_tag="ACG89C_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4900491.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACG89C_001963"
     CDS             8833..9099
                     /locus_tag="ACG89C_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS4900492.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACG89C_001964"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACG89C_001964"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4900493.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DAZUKR010000086        10724 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000086 DAZUKR010000000
VERSION     DAZUKR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.088"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..1089)
                     /locus_tag="ACG89C_001965"
     CDS             complement(241..1089)
                     /locus_tag="ACG89C_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4900494.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1253..2155
                     /gene="gnd"
                     /locus_tag="ACG89C_001966"
     CDS             1253..2155
                     /gene="gnd"
                     /locus_tag="ACG89C_001966"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS4900495.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="ACG89C_001967"
     CDS             2157..3158
                     /locus_tag="ACG89C_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS4900496.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="ACG89C_001968"
     CDS             3155..3439
                     /locus_tag="ACG89C_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4900497.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="ACG89C_001969"
     CDS             3472..4269
                     /locus_tag="ACG89C_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4900498.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="ACG89C_001970"
     CDS             4283..5116
                     /locus_tag="ACG89C_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4900499.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="ACG89C_001971"
     CDS             5134..5991
                     /locus_tag="ACG89C_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4900500.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="ACG89C_001972"
     CDS             6013..6522
                     /locus_tag="ACG89C_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4900501.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="ACG89C_001973"
     CDS             6559..6960
                     /locus_tag="ACG89C_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900502.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="ACG89C_001974"
     CDS             7072..7425
                     /locus_tag="ACG89C_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900503.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="ACG89C_001975"
     CDS             7459..7902
                     /locus_tag="ACG89C_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4900504.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="ACG89C_001976"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="ACG89C_001976"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4900505.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="ACG89C_001977"
     CDS             8997..9710
                     /locus_tag="ACG89C_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4900506.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="ACG89C_001978"
     CDS             complement(9917..10066)
                     /locus_tag="ACG89C_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900507.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="ACG89C_001979"
     CDS             10462..10662
                     /locus_tag="ACG89C_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4900508.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1695 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa attc
//
LOCUS       DAZUKR010000087        10707 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000087 DAZUKR010000000
VERSION     DAZUKR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.089"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG89C_001980"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG89C_001980"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4900509.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG89C_001981"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG89C_001981"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4900510.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG89C_001982"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG89C_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4900511.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG89C_001983"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG89C_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4900512.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG89C_001984"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG89C_001984"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4900513.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG89C_001985"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG89C_001985"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4900514.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG89C_001986"
     CDS             complement(8053..8523)
                     /locus_tag="ACG89C_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS4900515.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG89C_001987"
     CDS             complement(8569..8994)
                     /locus_tag="ACG89C_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS4900516.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG89C_001988"
     CDS             complement(9061..9330)
                     /locus_tag="ACG89C_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS4900517.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            9466..10545
                     /locus_tag="ACG89C_001989"
     CDS             9466..10545
                     /locus_tag="ACG89C_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4900518.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         2721 a         2453 c         1990 g         3543 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttag
     9361 gcggatttta taattaactt agccatttgg aaataaaaaa acgccacgtg aattccatgt
     9421 tatattgaag ttaccacaac tctcaataga caaggatgaa ttcacatgac gcaaaaccat
     9481 gataacacag tttcacgcaa gggaaaacac ctaacctact cagaacgttg ccaaatcgcc
     9541 atcctgaaaa aggaaaacta ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg
     9601 cagaccataa acaacgaaat caaacgcggg accgttactc agcttaaccg ccagaatcag
     9661 aacgggaagg tctacgacta ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc
     9721 tacgagaaga accgcttgaa ctgcggcaga cggcccttat gggccgacac ggacgccttc
     9781 attgaatggg cggacgacat gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc
     9841 tttgccagaa aacacgaatt gttcgacccg gccattatcc ctgggaccac caccctctat
     9901 cagtggatcg accgtggcat catgaaaacg aagaatatgg acctgctaga aaaactgtcc
     9961 cgcaaaccga aatcgtcccg tcatggcaac cgcccgaata aacgggtgct agggaaatcc
    10021 attgaagagc gcccggaggc ggttggtacc cggaaaacct tcggccattg ggaagtcgac
    10081 acggtcgtag gaaacaagac caagtcggat gccgtgctgc tgacgttggt ggaacggcag
    10141 acacgcttcg aggtcattct gaagctggcc gggaaggatg ccgaatcggt caaccaagcg
    10201 gtccgacagc tgagagaacg ggctggtgcg tacttcggcc agctgttcaa gacggttacc
    10261 tcggacaacg gctcggagtt cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc
    10321 tacttcagcc acccctatgc ttcctgggag cgcgggacga gcgaaaacca acacaagctg
    10381 attcgccgtt tcatccccaa gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg
    10441 cggattcagc gttggatgaa cgattacccg cgaaagattt tgggctatgc aacgccgcat
    10501 gacacgttcg tccaggcatt taggcaggaa cggttgacgg cttgacacgt gggcccttga
    10561 caacgagcct ccctcggcat ggcttgtgtc tggaaagctg gccggggcca gccagtcggg
    10621 agactaccat gcctcagctc attgtcaagg gcccactcga ttagaaaaat tagacggttg
    10681 gtggtaacga gcaaccgggt caccatg
//
LOCUS       DAZUKR010000088        10700 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000088 DAZUKR010000000
VERSION     DAZUKR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.090"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACG89C_001990"
     CDS             complement(25..162)
                     /locus_tag="ACG89C_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4900519.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG89C_001991"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG89C_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4900520.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACG89C_001992"
     CDS             complement(1599..1853)
                     /locus_tag="ACG89C_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900521.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACG89C_001993"
     CDS             complement(1878..3020)
                     /locus_tag="ACG89C_001993"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS4900522.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACG89C_001994"
     CDS             complement(3080..4432)
                     /locus_tag="ACG89C_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4900523.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACG89C_001995"
     CDS             complement(4503..4847)
                     /locus_tag="ACG89C_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4900524.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACG89C_001996"
     CDS             complement(4857..5231)
                     /locus_tag="ACG89C_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4900525.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACG89C_001997"
     CDS             complement(5244..5558)
                     /locus_tag="ACG89C_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4900526.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG89C_001998"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG89C_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACG89C_001999"
     CDS             complement(8976..9638)
                     /locus_tag="ACG89C_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900527.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ACG89C_002000"
     CDS             10255..10485
                     /locus_tag="ACG89C_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900528.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..>10700
                     /locus_tag="ACG89C_002001"
     CDS             10491..>10700
                     /locus_tag="ACG89C_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4900529.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDI"
BASE COUNT         2955 a         2365 c         1811 g         3569 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc
//
LOCUS       DAZUKR010000089        10466 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000089 DAZUKR010000000
VERSION     DAZUKR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.091"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..636
                     /locus_tag="ACG89C_002002"
     CDS             13..636
                     /locus_tag="ACG89C_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900530.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /gene="trpX"
                     /locus_tag="ACG89C_002003"
     CDS             1086..2135
                     /gene="trpX"
                     /locus_tag="ACG89C_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4900531.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="ACG89C_002004"
     CDS             2132..3013
                     /locus_tag="ACG89C_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4900532.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="ACG89C_002005"
     CDS             3016..3786
                     /locus_tag="ACG89C_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900533.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="ACG89C_002006"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="ACG89C_002006"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4900534.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="ACG89C_002007"
     CDS             5097..6260
                     /locus_tag="ACG89C_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4900535.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="ACG89C_002008"
     CDS             6377..6787
                     /locus_tag="ACG89C_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4900536.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..9189
                     /gene="topB"
                     /locus_tag="ACG89C_002009"
     CDS             6961..9189
                     /gene="topB"
                     /locus_tag="ACG89C_002009"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4900537.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9360..9644
                     /locus_tag="ACG89C_002010"
     CDS             9360..9644
                     /locus_tag="ACG89C_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900538.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9804..10040
                     /locus_tag="ACG89C_002011"
     CDS             9804..10040
                     /locus_tag="ACG89C_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4900539.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10037..10435
                     /locus_tag="ACG89C_002012"
     CDS             10037..10435
                     /locus_tag="ACG89C_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4900540.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3642 a         1763 c         2219 g         2842 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     9181 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     9241 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     9301 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     9361 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     9421 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     9481 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     9541 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc attatttatt
     9601 ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac gagagtttgt
     9661 gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa aagcaaaata
     9721 aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact atagaatgag
     9781 aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt agaaaatctg
     9841 gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt gtgcagacag
     9901 gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc aaagcacaag
     9961 aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa gacgcgttaa
    10021 aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa tcaacgcgaa
    10081 agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa atgcattaga
    10141 tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc ctgaaatcta
    10201 tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc ataatggcaa
    10261 taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt gtccagttgc
    10321 tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt cattggattt
    10381 cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa aatgaacgaa
    10441 ttaaaaactt tatttctcaa ttagat
//
LOCUS       DAZUKR010000090        10439 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000090 DAZUKR010000000
VERSION     DAZUKR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.092"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..1741)
                     /locus_tag="ACG89C_002013"
     CDS             complement(233..1741)
                     /locus_tag="ACG89C_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS4900541.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1900..2091
                     /locus_tag="ACG89C_002014"
     CDS             1900..2091
                     /locus_tag="ACG89C_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900542.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2294..3211
                     /locus_tag="ACG89C_002015"
     CDS             2294..3211
                     /locus_tag="ACG89C_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS4900543.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3204..4277
                     /locus_tag="ACG89C_002016"
     CDS             3204..4277
                     /locus_tag="ACG89C_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900544.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4425..4943
                     /locus_tag="ACG89C_002017"
     CDS             4425..4943
                     /locus_tag="ACG89C_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900545.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4924..5316
                     /locus_tag="ACG89C_002018"
     CDS             4924..5316
                     /locus_tag="ACG89C_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS4900546.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5392..6327
                     /locus_tag="ACG89C_002019"
     CDS             5392..6327
                     /locus_tag="ACG89C_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4900547.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6460..7239
                     /locus_tag="ACG89C_002020"
     CDS             6460..7239
                     /locus_tag="ACG89C_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4900548.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7232..7549
                     /locus_tag="ACG89C_002021"
     CDS             7232..7549
                     /locus_tag="ACG89C_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4900549.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7793..9403
                     /locus_tag="ACG89C_002022"
     CDS             7793..9403
                     /locus_tag="ACG89C_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4900550.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9560..10408
                     /locus_tag="ACG89C_002023"
     CDS             9560..10408
                     /locus_tag="ACG89C_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4900551.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3296 a         1825 c         2090 g         3228 t
ORIGIN      
        1 atcactccta ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt
       61 tagtgaaatt ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa
      121 gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa
      181 actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt
      241 aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca
      301 atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt
      361 cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg attgataaag
      421 cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca
      481 atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg
      541 ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac
      601 gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg
      661 taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt
      721 cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt
      781 cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt
      841 tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca
      901 tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata atacgtttga
      961 ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa
     1021 agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa
     1081 tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac
     1141 cagctgcatg tcctttttgg ctttcatgat acagttggtt caagcaagta gcgagctttc
     1201 cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat
     1261 tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt
     1321 ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg
     1381 caggctgtcc actatagcga gtgataacga cactgttgat taataagcca taaccacgta
     1441 attcatcgat taaccgcaag atatccatat cataagtaat cccataatca ccacggatct
     1501 tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt
     1561 tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct
     1621 tgtcgtcaac taacttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt
     1681 ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca
     1741 ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg
     1801 aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt
     1861 attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag
     1921 aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa
     1981 gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt
     2041 gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga
     2101 taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa
     2161 ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac
     2221 aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga
     2281 ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga
     2341 ggagacgaac gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga
     2401 aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt
     2461 ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt
     2521 aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga
     2581 tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag
     2641 ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct
     2701 gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga
     2761 gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac
     2821 agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa
     2881 cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa
     2941 gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga
     3001 aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc
     3061 agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt
     3121 cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca
     3181 atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg
     3241 aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc
     3301 ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacat gaaaaagaaa
     3361 aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa
     3421 atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa
     3481 cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc
     3541 cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg
     3601 gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg
     3661 gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt
     3721 atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac
     3781 gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg
     3841 tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg
     3901 cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac
     3961 ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc
     4021 agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa
     4081 atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag
     4141 gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg
     4201 gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa
     4261 tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt
     4321 ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa
     4381 atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa
     4441 tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta
     4501 ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa
     4561 aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg
     4621 gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg
     4681 tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg
     4741 cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc
     4801 aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg
     4861 ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa
     4921 aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca
     4981 agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg
     5041 caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtggtc
     5101 ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat
     5161 aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg
     5221 atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat
     5281 ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg
     5341 cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat
     5401 tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct
     5461 atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc
     5521 cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat
     5581 aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca
     5641 atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa
     5701 aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta
     5761 tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg
     5821 aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg
     5881 attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca
     5941 atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt
     6001 ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt
     6061 gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc
     6121 agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca
     6181 aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa
     6241 aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa
     6301 tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga
     6361 aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga
     6421 gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg
     6481 ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga
     6541 ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta
     6601 tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa
     6661 gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta tacattatta
     6721 ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct
     6781 caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc
     6841 tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc
     6901 aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt
     6961 gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc
     7021 tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact
     7081 ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca
     7141 ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc
     7201 ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt
     7261 tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca
     7321 tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc
     7381 ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat
     7441 caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt
     7501 gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt
     7561 ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg
     7621 caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta
     7681 tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt
     7741 gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa
     7801 aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg
     7861 ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc
     7921 tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt
     7981 cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc
     8041 tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc
     8101 tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt
     8161 taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct
     8221 tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt
     8281 gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt
     8341 attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg
     8401 tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact
     8461 taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc
     8521 tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat
     8581 gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca
     8641 gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct
     8701 gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa
     8761 aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat
     8821 tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt
     8881 tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt
     8941 ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat
     9001 ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt
     9061 agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg
     9121 cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt
     9181 tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt
     9241 tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc
     9301 tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg
     9361 aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt
     9421 tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa
     9481 ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg
     9541 aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc
     9601 ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg
     9661 gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa
     9721 cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga
     9781 ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc
     9841 tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc
     9901 ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt
     9961 aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa
    10021 tacctatgct tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac
    10081 aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga
    10141 agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga
    10201 agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca
    10261 tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa
    10321 tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc
    10381 catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaata
//
LOCUS       DAZUKR010000091        10360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000091 DAZUKR010000000
VERSION     DAZUKR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.093"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..612)
                     /locus_tag="ACG89C_002024"
     CDS             complement(127..612)
                     /locus_tag="ACG89C_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS4900552.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    702..1047
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1092..1733
                     /locus_tag="ACG89C_002025"
     CDS             1092..1733
                     /locus_tag="ACG89C_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4900553.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1752..2387
                     /locus_tag="ACG89C_002026"
     CDS             1752..2387
                     /locus_tag="ACG89C_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4900554.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2405..3220
                     /locus_tag="ACG89C_002027"
     CDS             2405..3220
                     /locus_tag="ACG89C_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4900555.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3539..4975
                     /locus_tag="ACG89C_002028"
     CDS             3539..4975
                     /locus_tag="ACG89C_002028"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4900556.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5164..7611
                     /locus_tag="ACG89C_002029"
     CDS             5164..7611
                     /locus_tag="ACG89C_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4900557.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7841..8908
                     /gene="rlmN"
                     /locus_tag="ACG89C_002030"
     CDS             7841..8908
                     /gene="rlmN"
                     /locus_tag="ACG89C_002030"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4900558.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8898..9104
                     /locus_tag="ACG89C_002031"
     CDS             8898..9104
                     /locus_tag="ACG89C_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900559.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9101..10204)
                     /locus_tag="ACG89C_002032"
     CDS             complement(9101..10204)
                     /locus_tag="ACG89C_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4900560.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3413 a         1778 c         2263 g         2906 t
ORIGIN      
        1 cgtattagtt ttcagcgttc atactagttg ttacattgtt tgattggcca aaagaatttc
       61 tgatatattt tgtgctaaat aaagaaaaag acgcttgaat tgggaaataa gaaaactact
      121 ctatcattac aaattttcat aaactggcag atgttctaag taaacaagta tcttttcaaa
      181 gtcggaaaaa tccataatca acgtagtggt attgatattt ggatgaaagc ctacggtatc
      241 ttgacgatca atagcagcat cgatcacgac ttggattttt ttctctttgt cgtgcatcaa
      301 tgctaatgga gtcactgtac ctgctggaac gcctaggagg tcaggaagct gttcttcaga
      361 aacaaaagat aagcgctttt cttttaattg ttcagctagt tgttttaaat cggcgtgctt
      421 ttcatcaggc aagatgacaa gatagatttg ttttcctttt cttgatttca ataatagatt
      481 ttttacttgc ggaccaggta gggaaaaagg gacatttttg actgacgtaa tcgcaggatg
      541 atcgactttt tgaaaagaga tacccagttt ttttagtagt tcataagctt cttgttcggt
      601 tgcacgactc atcgcgcatc accctccttt ttttattagt gtatcataaa aaagagttga
      661 caaatgaaaa ataatcatgt aaattacacg taaagttaaa acaaatgaca tcgaaaagaa
      721 gagtagccgt taggtgtttt tagagagtct ctggttggtg gaaagaggca gcagttaacc
      781 gtgaaacaca tcttggagtc gattttctga agcgtatagt aggaaaatcc ggggagcccg
      841 ttacagctgc ggtcgttgct agatgaccag ataaggctgg cttttccgtg aggagtcggt
      901 aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt cggatcaaag gactttttgt
      961 tttcatctga acggcaacga ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg
     1021 tggaaccacg tataatacgt cctcttggca tttgccaaga ggattttttt attttaaagg
     1081 aggaaaagaa gatggagtat ttgattgaag ttatgccgca attattagta ggagcactca
     1141 caacattgaa attattttta tttaccttga ttggctcatt accattaggg attgttttag
     1201 cattaggatt gtctttgcat tttaagccgc tacgctactt gcttcaaaca tatgtatggg
     1261 ttatgcgggg aacaccgctg ttgttgcaac tgatttttat tttttatggg ttgccgacga
     1321 ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc ctttatctta aactatgcag
     1381 cttattttgc agagatcttt cgtggaggga tacaatcgat tcctcaaggt caatatgaag
     1441 cagcgcaagt tttgcaatta agtccctttc aaacggtgac gaaaatcgtc atcccacaag
     1501 tgacaaagat tgttttacct tctgtaggga atgaagttat caacttggtc aaagattctt
     1561 ccttgatcta tatattaggt ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac
     1621 gcgacgcgac tttactgcct ttgctaggcg tggcaatcat ctatcttaca ttgactggta
     1681 ttctaacaat cataatgaaa caagttgaag gaaaattaaa ttattaccgc taactgatag
     1741 gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa
     1801 tcaaagattt gaatctaatc attccagatg ggaaaacatt agctatcgta ggtccttccg
     1861 gtggcggtaa aaccacgctt ttacgtatct tggctggctt agaaagttct gacagcggag
     1921 aattttattt agacggtgta ccgttcaacc cattcgagct taaaatgaaa gaacaagttg
     1981 taggagtcgt attccaagac ttccagctat ttccacatct gaatattttt gaaaacatta
     2041 ccttagcacc aaaaatggta ttgaaacaga caaaagaaga atacaccaaa aaagcagaag
     2101 aattagcggc ttcattaggg attagtgatt tattgaatat ctatccttat cagctttctg
     2161 gcggacaaaa acagcgggta gcgattgcca gagcattggc tatgaatcca cgggtattgg
     2221 cttatgatga accaacaagt gctttagatc ctgaattacg ccagcaagtc gagcgtttga
     2281 tcctatcatt gaaagaagat ggggtaaccc aaattattgt cacccatgat ttagtatttg
     2341 cagaaaatgt cggtgatcaa ttactaaaag tttccccaat tcattaagaa aggagaagat
     2401 agagatgaaa aaaaccagtt ttctattcgc tttgatcgca ggtcttcttc tttttgcagg
     2461 ctgttcgaac aaaaaaactt cagcagataa ttgggcgact atcaacgaag agaaaaaaat
     2521 cgttataggt ttagacgata catttgttcc tatgggattt agagataaag acgggaagct
     2581 cacaggattt gatattgatt tagccaaagc agtctttgaa caatatggga ttacggtaga
     2641 ctttcagccc attgactggt caatgaagga atttgaactg aacaatggaa cgatcgattt
     2701 gatatggaac ggctattcca aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc
     2761 ttatatggaa aacgatcaag tgctgatcac acctaaagaa tcaggaatca caaactttgg
     2821 acaaatgaat aataagagat taggtgcgca aaatgggtca tctggttatg atgtatttac
     2881 gaaacaacca gaagtattaa aagagatagt caaaaaccaa gatgctgttt tgtatgatag
     2941 ttttaatgaa gcattgatcg atctgaaaag cagacgggtc gatggtctgc tgatggataa
     3001 agtatacgca gactattatt tgaaacaaag aaacgaacta gaagacttca acataacaaa
     3061 aggttcttat gagagtgagg attttgcagt tggtgcacgc aagtcagata gtacattagt
     3121 gaaaaaaatc aatgaagccc tagatcaact agagaaaaca ggtgaattcc aaaaaatatc
     3181 cgataaatgg tttggcgaag atgttacgcc gagaaattaa caaatagggg actgtgacaa
     3241 aagacgtgtc acagtccctt tttccgaata aacagtgttc tcctgccaac tccttgtgac
     3301 tcggagctga atggcaggct cacaacctct tggtaaacgg tgttcaaaag ccagtttttc
     3361 ggtattaagc caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttact
     3421 actcgaaact aagcgacttt ttcacaacct cctttacata attttatttt gttgtggaag
     3481 gggtttcgtg ttattctaaa agaggaacga ttgaaggtta taggaaagga agagatgaat
     3541 ggaacacaaa gaacttacct catttccaaa agattttctg tggggctctg cttctgcagc
     3601 gtatcaagta gaaggagctt ggcaagaaga tgggaaagga gaatcagtct gggatcgatt
     3661 tgtacggata cctggaaaga catttaaagg aacgaacggt gatttggcag tagaccacta
     3721 tcatcgttat aaagaagata ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt
     3781 ctctgtagct tggacacgga tttttcctaa tggacgagga gaagtcaatc aagctgggct
     3841 cgctttttat gagagattga tcgacgaatt gattgaaaac gatattgaac ctatgttgac
     3901 tttatatcat tgggatcttc cacaagcatt gcaagatgaa tataatggtt gggaatcacg
     3961 tcagatcatt gaagatttca ctcattatgc agaaacatta tttgaggcat tccgcgggaa
     4021 agtccattac tggattagtt tgaatgaaca aaatatcttc acttcattag gttatttgtt
     4081 agcagcacat ccgccaggtg tgacagaccc gaaacgaatg tatgaagtta accatattgc
     4141 taatttagca aatgcatctg tcatcaataa atttcacgag ttagaaatgc ctggaaaaat
     4201 cggaccaagc tttgcgtata cgccaaatta tccaattgac agtaatcctg aaaatgttct
     4261 ggctgctgaa aatgcagaag acctgatggc caattattgg atggatgtct atatgtgggg
     4321 aaaatatccg attgctgcca tgaggttctt agaagaaaaa ggatgggcac caacgattga
     4381 agctggcgat gcagaattgc tagaatctgc taaaccagat tttcttggga tcaattacta
     4441 tcaaacggcg acgaatgcat tcaatccatt aaacggagtt ggtgctggaa aaatgaatac
     4501 gacaggaaaa aaaggttcat cagaagaaac tggagttcct ggaatgtata aaaaagttga
     4561 gaatccattt gtcgaacgaa caaattggga ctgggagatt gacccagaag gattaaggat
     4621 cggactgcgt cgtatcacaa gccgttaccg catgccggta ctgattacgg aaaatggctt
     4681 aggagagtat gacaagttga cagaagataa acaaatccat gacgactatc gaatcaatta
     4741 cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg gatggagcag aagtcctagg
     4801 ctattgcacc tggtcttata cagatttgct tagctggctg aacggttatc aaaagcgata
     4861 tggatttgta tatgttgatc aagacgaaac acaagaagga tctttggaac gatataagaa
     4921 agacagttat tactggtacc aaaaactgat tgaagaaaat ggagagaacc tttagttcac
     4981 atacttaaaa caagaaggta cggctggata tggccgtgcc ttttcttgat aaaatcattt
     5041 agaaattaag aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa
     5101 acaaaacttt agttccctta cttttttttg tataatgaaa cttgatgaaa tgagggataa
     5161 taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag
     5221 caaaaacagc agcaggaaca aatgctgacg attggtgttg ggtttgcctt gattttattt
     5281 gctgtcttcg gctttttaaa attaggtttt ttaggaatat tgattgcaaa cggctttcgg
     5341 atcattgcag gaaatactta ccaaatcctc tgtcttttat tagccgtatt aggattttgg
     5401 attgtcatta agaatacaga gttttcaatt ggtaaaaacc gacgctggtt tggcggcata
     5461 ctcttttatt tcggtatctt attattgtta catgcccatt tatttggcaa actccatact
     5521 ggggaaccaa atatcatggg gacgacatgg gatttgttag ccagtgatat aaaacaaagt
     5581 caagtagaca ataacgttgg tggtgggatg attggagcaa tcctttatca ttttacttac
     5641 ttcttgattg cacagcctgg aagttattta gcagcagtgc tattactggc aggaggagca
     5701 ttcctgttca gtaatctgga aggctatcag ctgttaaatg gtatccaatc aattggagaa
     5761 cgagtacagg aattattgga aggtgatcct gccaagcaag ctcgaaagca agcggcaaag
     5821 gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa
     5881 gctgcagaaa aagaagctgc agaatatgag aagaaaaaag cggtgagcca gcaaagggaa
     5941 aatactgctg atgaatggca ggaaccagaa aatacagagc cggagcagct aagttttgtt
     6001 ccaatcgaca gttttcaaga aaatatccat cctgcgaatc tagagaaacc agtaccagat
     6061 acaccaaaac agaccaatac agcagaaggt ttttcagatg agttgccaga ggatgacggt
     6121 acctcattag aatttgagat tgaagcagaa caggaaaatc aagactatga attaccgaca
     6181 gttgatttgc ttgattctat accaactgtg gatcaaagtg atgagtataa aaaaattgaa
     6241 aaaaatatcg gcgtattaga acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc
     6301 aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa
     6361 gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg ctttagcggc gaaagatgtc
     6421 cggatggaag caccgattcc aggtaaatca ctgattggta ttgaagtacc aaatggaaag
     6481 atcagtatgg tttcctttag agaaatcatc gaagcacaac caaaccaccc agataaatta
     6541 ctagaagtac ctttaggacg tgatgtttct ggacgggtac aaacggctga tctttcgaag
     6601 atgcctcact tactcgtggc tggttcgact ggtagtggga agtcagtagc aattaatggg
     6661 attatcacaa gtatattaat gcgtgctaag ccccatgaag taaaactgat gatgatcgat
     6721 ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc atctgttgac gccggtcgtg
     6781 acgaacccta gaaaagcagc gcaagcctta caaaaagtag tacaagaaat ggaagaacga
     6841 tatgaaaaat tcgcggcaac cggagtaaga aatatttctg gttacaatga atttgttcaa
     6901 cagaaaaact tggaaaacgg aacgaaacat ccaaccttac cattcatcgt agtgatcgta
     6961 gatgaattag ctgatttgat gatggtagcc agcaatgaag ttgaagatgc gattattcgt
     7021 ttagcgcaaa tggctcgggc agcggggatt catatgatcc tagctactca acgtccaagt
     7081 gtggatgtta tcacaggaat catcaaagca aatgtacctt ccagaatggc atttgcggta
     7141 tcaagcggta cagattctcg gacgatcatt gattctaatg gggcagaaaa gctcttaggg
     7201 cgaggagata tgctattctt gccaatgggt gagaataaac cgatccgtgt gcaaggagca
     7261 ttcatttctg accacgaagt agaacgggtc gttcaatttg ttacagatca gcaagaagct
     7321 cattatgaag agaaaatgat gccgacagat gaagttgaaa cgacaggtgc tccagaacag
     7381 ccacaagatg agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt
     7441 atttcccttc tccaaagaag atttcggatc ggttacaacc gagcagcacg tttagtagat
     7501 gaactggaag ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc
     7561 attgagcagg aagaaacatc agaaacgttg ccggacgata atgacatgta aaacttgtgt
     7621 gatgaagggt tgaaaaacga ataaccaata aaaagacaag gcatgatgat accaaaagaa
     7681 aaaatggttt ttttccatct tttctaatac ggtatgatgt cttgtctttt tagtttttgt
     7741 gagattaaag ttctattcga tttatttgtg aagtattgca ttaaggaaat gaatcatgta
     7801 tagtatgtag cgaaactttg agaaaaagag ggaaaatact ttggaaaaac agtccattta
     7861 tggcttgacg aatgaagaat taatcaattg gtttatagaa aacggcgaga aaaaattccg
     7921 agctgcccaa gtttgggaat ggttgtatca aaaacgtgta agtaatttca cagaaatgac
     7981 gaatctatcg aaacaattga tcgaaaaatt atcggagcat tttatcatca atcctctaaa
     8041 acagatggtc gtccaagaag cttcggatgg tacagtaaaa tatttgtttg aactccctga
     8101 taaaaacatg attgagactg tactgatgag acaagaatat ggcttgtcag tttgtgtgac
     8161 gacacaagta ggatgtaata tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa
     8221 ccgtgatctg actgctggag aaattgttgc acagatcatg atggtccaac attattttga
     8281 tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta atggggatcg gtgagccatt
     8341 tgataattac gacaatgtaa tggacttttt gcatatcatc aatgatgcca aaggattggc
     8401 aattggtgct cggcacatta cagtttccac tagtggatta gcacacaaaa tcaaggaatt
     8461 cgctaataac ggcttgcaag tcaatctggc tatttccttg cacgcgccga ataatgaggt
     8521 gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt gagaaattga tggaagctgt
     8581 ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt gaatatatca tgttgaatca
     8641 agtaaatgat agaccagagc atgcgcaaca attagcagat ctattaaaag ataagaaaaa
     8701 attagcatat gtcaacttga ttccatataa tccagtcagt gaacatgatc aatattctcg
     8761 cagtccaaaa gctgatgtat tgagatttta tgatgtattg aagaaaaacg gcgtaaactg
     8821 tgtgatacgt aaagaacatg gtacagatat tgatgcagct tgcggacaat tgcgaagcaa
     8881 gcaaatgaaa aaaacagttg ccaaataatc gtatcctact cgttttttca attaccataa
     8941 aaggagcagt tttcttaatt aaggaactgt tcaacttaaa taagagaaaa gattctatag
     9001 cgaaagctat aaagcttttt ttgacaagaa aaaccctcat caagcaaaga aatgtcctca
     9061 atttgatgac agcctgcttg atgggggttt tagcaattat ttaactgatt ttcttatatc
     9121 cttgctggct ttcttgtgta gcatgataag tttctacgtc aagaatggcc tgtttaaatt
     9181 tttctcgggt gatttcataa ggcacatgtt taatatcatt tgttgccatg gctttatcta
     9241 agattgcttc taaatcggtc gtgacattga gattcatgtc ggttaacgat actggaagtt
     9301 ggttcttttt cataaacaca tagattttat caaacaaatc gaactgctga tccatcatca
     9361 ataaggtcag tacaccataa ctgacagatg ctccgtgaag gtatccctcc attttttcta
     9421 gaacagtcgt accgtaatag aatgagtgtg ccatagcaga attatagtta ttgttaacta
     9481 aaacagaaac caatccggta gtaacaatga tatccgaaac gacttgttca attgcatatg
     9541 agggacgatc gtcggcacta tctttcaatg cttgcgtacc ataagtcaag agacgatcaa
     9601 tgcacatttt tccgatttgg attcctaatc tatccgtgta gcctagatca tcgccacgag
     9661 cagaaaaagt agattcgact tctttggata aagcatcgcc aatacctgcc cataagtatt
     9721 tatcaggtgc ttcggcaata attgctgtat caatgaaggt gtgtacaggt ggttttgaag
     9781 gaaagaagag tccgtccatt acgccttctt cgttatagat gacacatacc gcagtgacgg
     9841 gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg tttagctagt ttttcaccaa
     9901 tgacttttac agtatcgcaa gcacggccac caccgactgc aaacaggacg tctgcttttt
     9961 ggacactttc atgctggatc aactgttcaa tatttccata agttgaatta cccccgtacc
    10021 agagactggc gacaacctct atttcctgtg tcaaagctac ttttattttg ttttctgcct
    10081 ttgaaagtgc agtcttccct ccaatcagca ctacttttgt cccatattct ttgacgattc
    10141 catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa acactttgac
    10201 tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga gtgatcgatc
    10261 tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga tgaataaggt
    10321 ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
//
LOCUS       DAZUKR010000092        10331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000092 DAZUKR010000000
VERSION     DAZUKR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.094"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            220..495
                     /locus_tag="ACG89C_002033"
     CDS             220..495
                     /locus_tag="ACG89C_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900561.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            508..2580
                     /locus_tag="ACG89C_002034"
     CDS             508..2580
                     /locus_tag="ACG89C_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4900562.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2748..4631
                     /locus_tag="ACG89C_002035"
     CDS             2748..4631
                     /locus_tag="ACG89C_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4900563.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4643..5590
                     /locus_tag="ACG89C_002036"
     CDS             4643..5590
                     /locus_tag="ACG89C_002036"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4900564.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5610..6137
                     /locus_tag="ACG89C_002037"
     CDS             5610..6137
                     /locus_tag="ACG89C_002037"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4900565.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6200..6853)
                     /locus_tag="ACG89C_002038"
     CDS             complement(6200..6853)
                     /locus_tag="ACG89C_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4900566.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            6985..7827
                     /locus_tag="ACG89C_002039"
     CDS             6985..7827
                     /locus_tag="ACG89C_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4900567.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            7985..8881
                     /locus_tag="ACG89C_002040"
     CDS             7985..8881
                     /locus_tag="ACG89C_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4900568.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8884..9597
                     /locus_tag="ACG89C_002041"
     CDS             8884..9597
                     /locus_tag="ACG89C_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS4900569.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9617..10135
                     /gene="msrA"
                     /locus_tag="ACG89C_002042"
     CDS             9617..10135
                     /gene="msrA"
                     /locus_tag="ACG89C_002042"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4900570.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10132..>10331
                     /locus_tag="ACG89C_002043"
     CDS             10132..>10331
                     /locus_tag="ACG89C_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS4900571.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWE"
BASE COUNT         3585 a         1766 c         2143 g         2837 t
ORIGIN      
        1 aacaagaaag gcaggaatgg gaaagtaaac atccttttgt cagaaacttt tatctttctt
       61 aagttttact tgatatctga tactatttgg aatagaatga ggcgttgaaa aatttatgga
      121 aatttctaac tagcaataaa cgatgaaaca atgaaatatt tttcatccaa aaagaacttt
      181 gctataatga tggaagtttg tgtataggag atctggaaaa tgagagagag ttttgaacaa
      241 caaaaaaaac tgttgcatga tcgctatggc gccctttcga tggatgaccg ccgtcaaata
      301 ctttgtaagt tgcgtaaaag aaatatattg atgtatcgtc agttggaacg tctgaaacat
      361 gatctgcttc gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa ccaaacacag
      421 gttgaagtag tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa aatgcttaca
      481 caaaataaaa aataaagggg atggacgatg gaatttgctg agttagttat tgtatttgca
      541 gtaacgatca ccttatcgaa catagctagc cgcattttgc caatgattcc agctccactg
      601 atccaaattt tccttggagt ggtattaggt ctgacagagt ggggacaatc aatcgatttt
      661 gaacctgaat tgttcttagt tatgattatt gcaccattgt tatttagaga aggagaaaaa
      721 gcagatattt cttcgattct caaaaatttc gggacaattt tgttcttggc atttggagga
      781 gtcattttga cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc aagtgtccca
      841 cttgctgcct gctttgcttt tggagcagcg ttaggtccga cagatgcggt agcagtcagt
      901 tctctttctg gacgagtgaa tatccctaaa aaggcgatgc atattttgga aggagaaggt
      961 cttttgaatg atgcatctgg tgttactgcc ttccagtttg ctcttggtgc gctgatcacc
     1021 ggatcttttt cagcagtaaa cgcggggatg agtctagtag tttctagtat tgggggagct
     1081 ttgatcggct ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat cgaaaaagca
     1141 tcagcacaag atgtcacagg gtatctgctg atcgaacttt tgcttccttt tctagcctat
     1201 gtattggcag aggcatttga tgtatctgga atcattgctg cagtggcagc aggtattctc
     1261 caagcttctg gttttaggaa aatctctgtg tttgatgcag aattgtcgag cttgtcccac
     1321 agtacatgga caactattgc ttttacactg aatgcattag tatttatatt tttaggaatc
     1381 gaattgacgc aagtgttctc gccagtatgg ggagacggtt tatatccaaa cggtctttta
     1441 ttagcgatta tcgtattaat ctctgtcatg ctttttgtga tcagattcat ttctatcagt
     1501 cttttttatg tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa cgagatcttg
     1561 atcttaacat ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt tattttgcca
     1621 ttttcaatca ataatatgat gttttaccag cggtctttgc ttttgtttct gacggcgggt
     1681 gtcatcttag tgactttggt catagggatc attgtcttgc ctatgctgac ggaaacagag
     1741 gaagcgaaaa gcacggattt gaatgctttg atgattttag aagaggtcgt tgaagttttg
     1801 cgaaaagaaa taaaggaaat tgatcgtaat acaaaagaat ttttagccac tgaagcagtg
     1861 atagaaaatt atcaagaacg gatacgtgat ctgtacttgg aagatttaac cgatgatgaa
     1921 aagcaagaag tacaagaaat acaagcactg attttgtcga tcgaacggga tggattggat
     1981 gaaagttatc gttcgggcaa gctttcttct aatggttatc gtttttactc ccgttttctt
     2041 tctcgattcg aacattctat cacaagccag attttgtctt ttatcggctt ttggttcatt
     2101 gttgtccgta ggttaatgcg aatcgttctc catccgaaaa tgttttggca aagaagatat
     2161 gctgatcgtc agacatttat tagtgatgaa gatatccaag aaatcagaga gacatatcaa
     2221 aaaaatactc agttgattat tgaaagttta gataacttga ctgatattta tgatgaaacg
     2281 atggttcaat tttttatcca gcaacgcaaa cttgaaggaa taaaaatgaa ctcggggaat
     2341 ttgatttctt cgtggatgat ccagcaagat agtctgttta cgaaaaaaat gctaagaggt
     2401 tattatcttg aaagaaaaat cattgatgaa tacgaagttg ctgaaaaaat cacgacgttt
     2461 tctgcgaaca gctatcgcag gaatatcaat ttgttagaat cttatacgat gaacaaacca
     2521 tctgataact tttctttccg atttgcgttt cgagcaaagt caaaaaaacg agtccaataa
     2581 aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga acaggatcga
     2641 atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa attttcctga
     2701 agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg aaagaattaa
     2761 aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag atttcttttt
     2821 tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc
     2881 tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc
     2941 caactgatta ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg
     3001 ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg
     3061 aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta
     3121 aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta
     3181 ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc
     3241 agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag
     3301 acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac
     3361 aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa
     3421 accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt
     3481 acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc
     3541 ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc
     3601 agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag
     3661 aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca
     3721 aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc
     3781 aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta
     3841 atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc
     3901 atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt
     3961 acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag
     4021 aattacttga acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt
     4081 caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt
     4141 tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt
     4201 attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca
     4261 aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa
     4321 acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac
     4381 caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag
     4441 aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag
     4501 aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat
     4561 ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg
     4621 tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt
     4681 ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata
     4741 ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt
     4801 tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct
     4861 attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc
     4921 agactataat ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt
     4981 caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc
     5041 ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga
     5101 aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac
     5161 tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc
     5221 tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca
     5281 attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc
     5341 gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac
     5401 attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg
     5461 agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt
     5521 tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat
     5581 cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa
     5641 acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct
     5701 tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg
     5761 gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag
     5821 cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag
     5881 acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta
     5941 cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg
     6001 ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc
     6061 gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag
     6121 gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc
     6181 catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac
     6241 ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa
     6301 agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc
     6361 caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga
     6421 tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa
     6481 tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga
     6541 tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat
     6601 cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg
     6661 ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata tggagggagg
     6721 aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc
     6781 caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt
     6841 tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg
     6901 tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg
     6961 ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt
     7021 acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct atccatcatg
     7081 atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg
     7141 atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg
     7201 atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc
     7261 ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg
     7321 gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa
     7381 ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac
     7441 aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc
     7501 agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc
     7561 cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt
     7621 aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat
     7681 gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac
     7741 atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg
     7801 tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc
     7861 ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata
     7921 aagactacct ttttgataaa atttttgata acattagaac caatcgaata caagaggtga
     7981 aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat taattgcgat
     8041 catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa tgaaaccagt
     8101 aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag ctatcggaga
     8161 ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc cccttgtcgc
     8221 aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact atggtgtaaa
     8281 tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa
     8341 tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga tgaaagttat
     8401 ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga aaaactatca
     8461 ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg cacctattta
     8521 cattttaggc atctacaatc cattttattt gaactttcct gagatcactg acatgcagac
     8581 aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta
     8641 ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag gtgtcacaga
     8701 agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca aaaataatgt
     8761 gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga tggcaaatgc
     8821 agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg atgatcaata
     8881 aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa
     8941 ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt aggcagccgt
     9001 atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga aagacaagga
     9061 gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat cagctttttc
     9121 cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga aaatgaagca
     9181 ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt atattttgat
     9241 ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca
     9301 ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa attaccagat
     9361 tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca attccgaatg
     9421 caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga cattcggttt
     9481 agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac caacaaaact
     9541 agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga tgagtagtat
     9601 tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct ggtgtatgat
     9661 ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata ctggagggca
     9721 tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata cagaagcagt
     9781 ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac tttactggca
     9841 gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca attatcgccc
     9901 ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta agaaacagtt
     9961 agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag cccaaccatt
    10021 ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa attttaaaag
    10081 aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag ggtgaatcga
    10141 agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc tttatctgtt
    10201 ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag ctacgaagaa
    10261 atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat ctttgacgaa
    10321 gcatgggaaa a
//
LOCUS       DAZUKR010000093        10181 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000093 DAZUKR010000000
VERSION     DAZUKR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.095"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..2542
                     /locus_tag="ACG89C_002044"
     CDS             101..2542
                     /locus_tag="ACG89C_002044"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS4900572.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="ACG89C_002045"
     CDS             complement(2872..3576)
                     /locus_tag="ACG89C_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900573.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="ACG89C_002046"
     CDS             complement(3763..4008)
                     /locus_tag="ACG89C_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900574.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="ACG89C_002047"
     CDS             complement(4025..4876)
                     /locus_tag="ACG89C_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4900575.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="ACG89C_002048"
     CDS             5042..6046
                     /locus_tag="ACG89C_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS4900576.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6829..7386)
                     /locus_tag="ACG89C_002049"
     CDS             complement(6829..7386)
                     /locus_tag="ACG89C_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900577.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(7565..8176)
                     /locus_tag="ACG89C_002050"
     CDS             complement(7565..8176)
                     /locus_tag="ACG89C_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900578.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(8289..8546)
                     /locus_tag="ACG89C_002051"
     CDS             complement(8289..8546)
                     /locus_tag="ACG89C_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900579.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(9143..9421)
                     /locus_tag="ACG89C_002052"
     CDS             complement(9143..9421)
                     /locus_tag="ACG89C_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900580.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(9455..9706)
                     /locus_tag="ACG89C_002053"
     CDS             complement(9455..9706)
                     /locus_tag="ACG89C_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900581.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         3199 a         1892 c         1840 g         3250 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     6181 taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg agttaaacgc
     6241 ttctgtccca gcctccttat tccgaataaa tggtgtccaa aagcttgcat ctgccagatt
     6301 atctcatttc ttatgacaag ctaagcaaat tatctccacc aaataattta ttcatatttt
     6361 tgacttgcgc atactaacta attcttaaat tttcatacaa aatttctcaa tttcccttaa
     6421 cgagtaatat ttatgtacgg tgattctcac tagtatccca atcacttttt aagttatccc
     6481 gatttcaatt ctacttcaaa aatagttgct tttatgtatg ctgttagttg attagatggt
     6541 catttgttga gctaggtcgt ttattttaaa caagttgttt ctttagctta tacctatctc
     6601 gtctaccaaa cgcaagatgg caaacacaaa tgcatacgca tctgtatttg ccactttgat
     6661 tcgtataaag ctaacaacta ttaactcctc tcagctaata gttgctctct tttttattca
     6721 gattcatttt tgctgatcac agcctagtat ttcaatggtt ttgaacacta aaccaaattg
     6781 ttccattaca acattcactg gctgatgaaa tggattagta gatttcgatt attcaaagcg
     6841 attttctatt ggtggatcgt gttcccaact aataagacgg attccaaacc tatcgtacag
     6901 atcttctaaa ccacttataa attttgcacc tatttcaggt gttttattgc gtggatattc
     6961 tattgttacg ccattaaatg gataccaatt aggattaggc tctctttttt tctgtacatc
     7021 tcccatagaa tagttagcac tttctgggac ataatatatt cttcgaccat cctctaattc
     7081 atctaataag atatttcgtg atcgtctatc acttgctaaa tgtagacgcc acgtgcgata
     7141 ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc
     7201 tatcacaatc ctttccccat ctacttcata ttctaatcta aaatcaaact cccctcgggt
     7261 aatttctggt cttggtggct taggacccaa tattattcta cttaaaaccg cattcgttcc
     7321 cagatgaaac acagctataa caacaataat gattaatatt ttgtttctat ttgataaatt
     7381 tctcattatt cgcacatcac ctctacattc tcacatatta gttcgtaata ctcctttaat
     7441 atcaaaatat ctactggtcg taataatatt cgctatgaat tcctactact cgtataagaa
     7501 aggccaaatc atcagctttt acttgctgat gatttggctt gtttctattt atcgtaattg
     7561 ttttctaatc accaaaattg ttaacaatcg gttcagatat ttcccagttg attaaggtaa
     7621 ttccgtattg tgtcaaaact tcttgcgctt ctccgaaatt aagtacagtt cttatgtatc
     7681 cactttctcc taatataaag tctacctctc ttttaaaacc ttcctcgaga tttctaaggg
     7741 ctatatgtgg ttttaatgac attgattgta cattttcccc catataatat tgcgcacttc
     7801 ctcgactaaa atagattcct agatcgtccc caaaaggtag atcttctcta ccactagcta
     7861 atctacttct ccatcttcgt tctcttttca tagaacctgc gctgaactca cacccgttaa
     7921 actcagctat gacagtatct tcaattatgt taatctgtcc atctagttcg tattcaatcc
     7981 taaatggaaa ttctccatag gtgatttccg gttcgggtat tttaggtgag attaatgcaa
     8041 agaaaggaaa tattaagctt ggcaacatca aaaaaactcc tcctatgatc acaattcccg
     8101 atgtaatcca aaatataatt tgtacaattc tcattgatcc attgtattcc attttattcc
     8161 ccctattact attcatatcc gcctatctat atcgatcttc caatgcagag ataattgctt
     8221 ccataccttg attttttact gagttaccta tgcgagatat cattctccac taaggtgcat
     8281 catattgtct atctaacatg attaattctc caataggtgt atactcctcc aaaaacaaca
     8341 tttaatgcat ctccctcgac aacataagct accatgtttg ctgtatatgt gtttgagtct
     8401 aattcataag cagataaatt tactgtcgct ggattaaata taatcgcatt cgtatttata
     8461 gctactgaat ttgctgcttc agcaccacct tttgaatgtc cgatcattgt gatttcaata
     8521 gtaggattta cgtggacagc cctcaccgtt taccctgacc attatagaaa tcgcttactt
     8581 ttttgaacta cgcaacaaag gatacaatgc tcctaacttc actgtctcca tttttgtcca
     8641 ctaaggaata taaaaaatag gcattaattc atttaattcg gtcacaacac gttacctttt
     8701 aaatcattat actgtatctc atacattatt atcaataaac tattataaaa atgcaaaaaa
     8761 tgctttatca atctattttg taattggtca taatatcatt gaaatcatac cctgtatcga
     8821 tttttcctat gaatctcgtc tatttgtgat tgacgaaaaa gaggcaagac agacactgga
     8881 atggtaattt tacccactat tttgctccaa ctatttgtaa tcgtacccac ttttgaaata
     8941 ccgagatgat cattttaatt gatatgctga ttcccaagca aaaccccaag aaattaaatg
     9001 ctctatcgca tctagcttct tgggttttgc ttatataaat tccaacattt aaatttttta
     9061 tgctgttcgg tctcagaaat gccgtttttg cttatatcac aatatcaaga tgtcaaatta
     9121 ttcgtgttgc cgatgacttt gattaatttt tttcaaattg tttattaaga ctaatgctgt
     9181 ctttttgcag ataccaatgt tcgaactttt taacattatc atatgatcct gcaagtttta
     9241 acttcttacc attagatgtt atcaaagtta taattaacct tgcctttttc ccattaggat
     9301 cttttatttt atgaattttc tgcaggtaat ctctcctaat ggcagctatt tttacctcat
     9361 cagtgctgat aaaccattga tcatccacat acatactgcc tgttatgttg tgtttttcca
     9421 tttcttccga gaacgaaaag tttaaggctc gttcttactt ccaaagttga atgagtccat
     9481 ggattggact tagtgtgaga attgagaaca acaataagaa tccatatcct cccgcaaaca
     9541 tccctaaggc tatactgtct ctccatccat aagtaggatt agaagagaaa agagtcaccg
     9601 tttgtaataa tatgaaaagg aataatccag cacaaacacc aaagataaca actccaaaca
     9661 ttgaattttt atacaatgac catttattta taggcttttc tttcatttta cttcccccta
     9721 ctgttctgtt cacacggaat gaattcaaag ttcataagcc ctcctctttg aacatatgaa
     9781 tacgacttgc taactatact ttaaaaaagc ttgttttaag cggttatagg cgagttgtaa
     9841 tctctattca tctcattagc aaataacttg ccgaaaagtc tactgaaatt aaagcagata
     9901 gtggaagaat tatttagcca ggatagacac tatttgtgaa acacatgtct ttacttttga
     9961 aattctatga tgaaaagcct tggaatacag tagctcttaa ttggtaatac tttttccatt
    10021 ataaataacc tcttttcatt ttatatttat cccctctaaa aataatatct aatctaatat
    10081 tttaaactta tatttccctc gctgtgctaa tagagcatta ctaaataaat agaaactaga
    10141 aaaaagcatt acgcctgcca gaataaacca attatttgta t
//
LOCUS       DAZUKR010000094        10130 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000094 DAZUKR010000000
VERSION     DAZUKR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.096"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            276..545
                     /gene="rpsN"
                     /locus_tag="ACG89C_002054"
     CDS             276..545
                     /gene="rpsN"
                     /locus_tag="ACG89C_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4900582.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            560..739
                     /gene="rpmF"
                     /locus_tag="ACG89C_002055"
     CDS             560..739
                     /gene="rpmF"
                     /locus_tag="ACG89C_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4900583.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            771..1148
                     /locus_tag="ACG89C_002056"
     CDS             771..1148
                     /locus_tag="ACG89C_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900584.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1169..1318
                     /gene="rpmG"
                     /locus_tag="ACG89C_002057"
     CDS             1169..1318
                     /gene="rpmG"
                     /locus_tag="ACG89C_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4900585.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1342..2325
                     /locus_tag="ACG89C_002058"
     CDS             1342..2325
                     /locus_tag="ACG89C_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4900586.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2355..2477
                     /locus_tag="ACG89C_002059"
     CDS             2355..2477
                     /locus_tag="ACG89C_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4900587.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2495..4018
                     /locus_tag="ACG89C_002060"
     CDS             2495..4018
                     /locus_tag="ACG89C_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4900588.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4042..4281
                     /locus_tag="ACG89C_002061"
     CDS             4042..4281
                     /locus_tag="ACG89C_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900589.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4474..4935)
                     /locus_tag="ACG89C_002062"
     CDS             complement(4474..4935)
                     /locus_tag="ACG89C_002062"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4900590.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5130..5348)
                     /locus_tag="ACG89C_002063"
     CDS             complement(5130..5348)
                     /locus_tag="ACG89C_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900591.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5466..7082
                     /locus_tag="ACG89C_002064"
     CDS             5466..7082
                     /locus_tag="ACG89C_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4900592.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7323..8663)
                     /locus_tag="ACG89C_002065"
                     /pseudo
     CDS             complement(7323..8663)
                     /locus_tag="ACG89C_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9101..9247)
                     /locus_tag="ACG89C_002066"
     CDS             complement(9101..9247)
                     /locus_tag="ACG89C_002066"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900593.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9286..9480)
                     /locus_tag="ACG89C_002067"
     CDS             complement(9286..9480)
                     /locus_tag="ACG89C_002067"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4900594.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9390..9722)
                     /locus_tag="ACG89C_002068"
     CDS             complement(9390..9722)
                     /locus_tag="ACG89C_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4900595.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3369 a         1606 c         2003 g         3152 t
ORIGIN      
        1 tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa agtgaattct
       61 tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga
      121 taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt
      181 gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag
      241 gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta
      301 aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag
      361 ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga
      421 ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc
      481 gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt
      541 ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg
      601 gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg
      661 tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat
      721 taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta
      781 tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc
      841 tagataaatg cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt
      901 taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct
      961 tatcatttga tgatttttta agaatctatc caatttttga agagtatgac gaggagttat
     1021 ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac
     1081 aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag
     1141 atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga
     1201 aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga
     1261 attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct
     1321 aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg
     1381 gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa
     1441 attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat
     1501 agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat
     1561 ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca
     1621 caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca aacactttta
     1681 acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt
     1741 gacagttttg aacttattga aagagaacca caagccctta agcaacttgt catggcagat
     1801 cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga
     1861 gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt
     1921 caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat
     1981 tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt
     2041 aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt
     2101 gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta
     2161 caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac
     2221 accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa
     2281 aggatcaaac agagttttga taaattactg gcggaaagca aataatgata agtagtgatt
     2341 gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc
     2401 cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa
     2461 tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat
     2521 tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc
     2581 gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac
     2641 caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc
     2701 tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt
     2761 atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc
     2821 tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga
     2881 agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc
     2941 acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc
     3001 gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt
     3061 ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca
     3121 cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact
     3181 aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa
     3241 aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt
     3301 ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga
     3361 acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga
     3421 aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa
     3481 gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta
     3541 tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt
     3601 cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac
     3661 tactggctat aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt
     3721 agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta
     3781 tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg
     3841 ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca
     3901 tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac
     3961 tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa
     4021 ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat
     4081 attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta
     4141 gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg
     4201 gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg
     4261 cataatagct tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga
     4321 tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga
     4381 agacttcata aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa
     4441 tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt
     4501 ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg
     4561 aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt
     4621 tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat
     4681 cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa
     4741 gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat
     4801 atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat
     4861 cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt
     4921 tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc
     4981 gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg
     5041 ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg
     5101 acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct
     5161 tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc
     5221 agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa
     5281 aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac
     5341 tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc
     5401 gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt
     5461 tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga
     5521 tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc attgtaaatg
     5581 acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg
     5641 gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga
     5701 ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt
     5761 caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag
     5821 acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac
     5881 gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg
     5941 tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca
     6001 tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc
     6061 taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact
     6121 tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct
     6181 ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta
     6241 tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta
     6301 cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac
     6361 atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa
     6421 attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg
     6481 aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg
     6541 gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc
     6601 ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta
     6661 gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa
     6721 ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg
     6781 agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa
     6841 tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg
     6901 gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc
     6961 ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt
     7021 taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact
     7081 aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca
     7141 ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc
     7201 acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt
     7261 tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac
     7321 ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta
     7381 ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
     7441 aagtttctat agccataggc aattcgtttt agtactttga tatggttatt caaacattct
     7501 atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg
     7561 aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc
     7621 gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg
     7681 tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc
     7741 agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc
     7801 ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
     7861 gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga
     7921 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
     7981 gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag
     8041 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
     8101 ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca
     8161 tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg
     8221 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata
     8281 gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag
     8341 cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     8401 cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa taagatggat
     8461 acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat
     8521 tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attgattcgc
     8581 tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc tagtaactct
     8641 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     8701 ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     8761 cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc
     8821 aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat
     8881 cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg attaattctc
     8941 atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc
     9001 tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa gaccgatgct
     9061 ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac
     9121 ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga atacgagttg
     9181 ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat
     9241 ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac atttttttac
     9301 catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa atcctcttgt
     9361 gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt cgattccttc
     9421 atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt cgtagaacac
     9481 tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc tgctttatca
     9541 ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata ttctgaaacc
     9601 catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt ggtagttact
     9661 tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata tgtttttctc
     9721 aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca
     9781 acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag tttttaacaa
     9841 ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac atagttgaat
     9901 aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag gagaagtcct
     9961 cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat acgtaaataa
    10021 agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac gaattaaaaa
    10081 acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa
//
LOCUS       DAZUKR010000095        10128 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000095 DAZUKR010000000
VERSION     DAZUKR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.097"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACG89C_002069"
     CDS             complement(247..759)
                     /locus_tag="ACG89C_002069"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4900596.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG89C_002070"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG89C_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4900597.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACG89C_002071"
     CDS             complement(3212..3655)
                     /locus_tag="ACG89C_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS4900598.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACG89C_002072"
     CDS             complement(3656..4444)
                     /locus_tag="ACG89C_002072"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4900599.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG89C_002073"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG89C_002073"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS4900600.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACG89C_002074"
     CDS             complement(5559..6146)
                     /locus_tag="ACG89C_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS4900601.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG89C_002075"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG89C_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS4900602.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACG89C_002076"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACG89C_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4900603.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACG89C_002077"
     CDS             complement(9063..9998)
                     /locus_tag="ACG89C_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4900604.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2798 a         2302 c         1753 g         3275 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataacccgc gaattgtaaa attaagttag aaaaataaaa aggcattt
//
LOCUS       DAZUKR010000096        10114 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000096 DAZUKR010000000
VERSION     DAZUKR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.098"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..625
                     /locus_tag="ACG89C_002078"
     CDS             119..625
                     /locus_tag="ACG89C_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4900605.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="ACG89C_002079"
     CDS             642..1541
                     /locus_tag="ACG89C_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS4900606.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="ACG89C_002080"
     CDS             1628..2512
                     /locus_tag="ACG89C_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4900607.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="ACG89C_002081"
     tRNA            complement(2704..2775)
                     /locus_tag="ACG89C_002081"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="ACG89C_002082"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="ACG89C_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4900608.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="ACG89C_002083"
     CDS             4318..5409
                     /locus_tag="ACG89C_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS4900609.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="ACG89C_002084"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="ACG89C_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4900610.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /gene="pgk"
                     /locus_tag="ACG89C_002085"
     CDS             6561..7751
                     /gene="pgk"
                     /locus_tag="ACG89C_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4900611.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /gene="tpiA"
                     /locus_tag="ACG89C_002086"
     CDS             7855..8610
                     /gene="tpiA"
                     /locus_tag="ACG89C_002086"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4900612.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="ACG89C_002087"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="ACG89C_002087"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4900613.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3541 a         1871 c         2095 g         2607 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaa
//
LOCUS       DAZUKR010000097        10084 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000097 DAZUKR010000000
VERSION     DAZUKR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.099"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACG89C_002088"
     CDS             162..392
                     /locus_tag="ACG89C_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS4900614.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACG89C_002089"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACG89C_002089"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4900615.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACG89C_002090"
     CDS             1803..1979
                     /locus_tag="ACG89C_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4900616.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACG89C_002091"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACG89C_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4900617.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="ACG89C_002092"
     CDS             2474..2941
                     /locus_tag="ACG89C_002092"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900618.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="ACG89C_002093"
     CDS             3026..3910
                     /locus_tag="ACG89C_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4900619.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="ACG89C_002094"
     CDS             4024..4260
                     /locus_tag="ACG89C_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS4900620.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="ACG89C_002095"
     tRNA            4348..4428
                     /locus_tag="ACG89C_002095"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="ACG89C_002096"
     tRNA            4435..4506
                     /locus_tag="ACG89C_002096"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="ACG89C_002097"
     CDS             4631..4801
                     /locus_tag="ACG89C_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900621.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="ACG89C_002098"
     CDS             5021..5728
                     /locus_tag="ACG89C_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4900622.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="ACG89C_002099"
     CDS             complement(5788..6432)
                     /locus_tag="ACG89C_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS4900623.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="ACG89C_002100"
     CDS             6559..6846
                     /locus_tag="ACG89C_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900624.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="ACG89C_002101"
     CDS             6954..7274
                     /locus_tag="ACG89C_002101"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4900625.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="ACG89C_002102"
     CDS             7355..8668
                     /locus_tag="ACG89C_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4900626.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="ACG89C_002103"
     CDS             8665..9384
                     /locus_tag="ACG89C_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS4900627.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="ACG89C_002104"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="ACG89C_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4900628.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DAZUKR010000098        10022 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000098 DAZUKR010000000
VERSION     DAZUKR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.100"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(486..710)
                     /locus_tag="ACG89C_002105"
     CDS             complement(486..710)
                     /locus_tag="ACG89C_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900629.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            901..3678
                     /locus_tag="ACG89C_002106"
     CDS             901..3678
                     /locus_tag="ACG89C_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4900630.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3759..4178
                     /locus_tag="ACG89C_002107"
     CDS             3759..4178
                     /locus_tag="ACG89C_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4900631.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4190..4660
                     /locus_tag="ACG89C_002108"
     CDS             4190..4660
                     /locus_tag="ACG89C_002108"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4900632.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4677..5456
                     /locus_tag="ACG89C_002109"
     CDS             4677..5456
                     /locus_tag="ACG89C_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4900633.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5443..6264
                     /locus_tag="ACG89C_002110"
     CDS             5443..6264
                     /locus_tag="ACG89C_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4900634.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6281..7330
                     /locus_tag="ACG89C_002111"
     CDS             6281..7330
                     /locus_tag="ACG89C_002111"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4900635.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7343..8347
                     /locus_tag="ACG89C_002112"
     CDS             7343..8347
                     /locus_tag="ACG89C_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4900636.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8980..9999
                     /locus_tag="ACG89C_002113"
     CDS             8980..9999
                     /locus_tag="ACG89C_002113"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4900637.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1651 c         2094 g         2915 t
ORIGIN      
        1 taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa
       61 atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt
      121 gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta
      181 tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt
      241 gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt
      301 aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag
      361 tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt
      421 tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt
      481 gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa
      541 aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata
      601 gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga
      661 aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca
      721 tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg
      781 ttttttagaa taaaaattat aagggataag taggctgctg tctataataa gatatatttt
      841 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
      901 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
      961 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
     1021 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
     1081 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
     1141 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
     1201 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
     1261 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
     1321 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
     1381 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
     1441 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
     1501 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
     1561 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
     1621 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
     1681 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
     1741 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
     1801 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
     1861 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
     1921 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
     1981 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
     2041 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
     2101 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
     2161 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
     2221 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
     2281 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
     2341 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
     2401 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
     2461 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
     2521 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
     2581 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
     2641 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
     2701 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
     2761 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
     2821 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
     2881 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
     2941 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
     3001 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
     3061 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
     3121 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
     3181 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
     3241 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
     3301 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
     3361 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
     3421 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
     3481 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
     3541 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
     3601 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
     3661 atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc
     3721 ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag
     3781 cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa
     3841 atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag
     3901 taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg
     3961 gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg
     4021 ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga
     4081 caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg
     4141 agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt
     4201 gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat
     4261 aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac
     4321 aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc
     4381 gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc
     4441 gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg
     4501 agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga
     4561 agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt
     4621 accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga
     4681 ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag
     4741 ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg
     4801 atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct
     4861 cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg
     4921 ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt
     4981 tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag
     5041 cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg
     5101 gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca
     5161 atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag
     5221 ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac
     5281 aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga
     5341 ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc
     5401 gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc
     5461 aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg
     5521 gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg
     5581 attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta
     5641 gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg
     5701 gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca
     5761 tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg
     5821 attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg
     5881 ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga
     5941 tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg
     6001 acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc
     6061 accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt
     6121 tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta
     6181 ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt
     6241 ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta
     6301 tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag
     6361 agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc
     6421 ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga
     6481 acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt
     6541 gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg
     6601 gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca
     6661 aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc
     6721 gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag
     6781 caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc
     6841 gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca
     6901 gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa
     6961 attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg
     7021 cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa
     7081 taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac
     7141 cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga
     7201 atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt
     7261 agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag
     7321 taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat
     7381 aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga
     7441 aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc
     7501 tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata
     7561 ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac
     7621 cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact
     7681 gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac
     7741 ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta
     7801 tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc
     7861 aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa
     7921 acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta
     7981 ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc
     8041 tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt
     8101 tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat
     8161 ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa
     8221 atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc
     8281 tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga
     8341 ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc
     8401 cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc
     8461 tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg
     8521 caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc
     8581 gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa
     8641 ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg
     8701 tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa
     8761 tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg
     8821 taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa
     8881 agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact
     8941 ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt
     9001 cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat
     9061 gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat
     9121 cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca
     9181 gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca
     9241 gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa
     9301 ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa
     9361 gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta
     9421 catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa
     9481 aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa
     9541 actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga
     9601 ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt
     9661 ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg
     9721 atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag
     9781 ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg
     9841 acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca
     9901 atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta
     9961 cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc
    10021 gt
//
LOCUS       DAZUKR010000099         9841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000099 DAZUKR010000000
VERSION     DAZUKR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.101"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..3251
                     /gene="fss3"
                     /locus_tag="ACG89C_002114"
     CDS             24..3251
                     /gene="fss3"
                     /locus_tag="ACG89C_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4900638.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3364..3678
                     /locus_tag="ACG89C_002115"
     CDS             3364..3678
                     /locus_tag="ACG89C_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4900639.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3691..4065
                     /locus_tag="ACG89C_002116"
     CDS             3691..4065
                     /locus_tag="ACG89C_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4900640.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4075..4410
                     /locus_tag="ACG89C_002117"
     CDS             4075..4410
                     /locus_tag="ACG89C_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS4900641.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4493..5833
                     /locus_tag="ACG89C_002118"
     CDS             4493..5833
                     /locus_tag="ACG89C_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4900642.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5911..6585
                     /locus_tag="ACG89C_002119"
     CDS             5911..6585
                     /locus_tag="ACG89C_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4900643.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6578..6742
                     /locus_tag="ACG89C_002120"
     CDS             6578..6742
                     /locus_tag="ACG89C_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900644.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6818..7960
                     /gene="dcm"
                     /locus_tag="ACG89C_002121"
                     /pseudo
     CDS             6818..7960
                     /gene="dcm"
                     /locus_tag="ACG89C_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            7950..8240
                     /locus_tag="ACG89C_002122"
     CDS             7950..8240
                     /locus_tag="ACG89C_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900645.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8499..9683
                     /gene="mobT"
                     /locus_tag="ACG89C_002123"
     CDS             8499..9683
                     /gene="mobT"
                     /locus_tag="ACG89C_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4900646.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9680..9817
                     /locus_tag="ACG89C_002124"
     CDS             9680..9817
                     /locus_tag="ACG89C_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4900647.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3256 a         1662 c         2236 g         2687 t
ORIGIN      
        1 ttaatttgaa aggagaacat ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa
       61 ctattttgag tttggctgtt caagcaattg ttggagcaat tgctcctacg attacttttg
      121 ctgatgaaat tacccatcca caaacggtaa ccgtagaact agatttagca catcaatatg
      181 ctgttgaagg aacttttagt gatggtcgtc caatgtcaga agtcactgtt ccgcattatg
      241 cagtgtacaa cggctttaaa caagatattt tctgtattga accaggagtc ccgatttaca
      301 atgagttcac tcctggctat gagaaaaacc cgttaccaga tatgtccgat aaagccaaat
      361 tagtttccgt tttatggaaa aaagtaggta cagatgcaga tactcatatt gtggcacaga
      421 agatgatctg gcaagaagtt aatgggtata cccttcactc aataaaacga tcagatggta
      481 gtgccgtaaa tatcgcagcc attgaggcaa aaataaatca agccattgac gattatcaga
      541 agaaaccaac cttccataat agtacggcta aaacagtatt agggcaatcg actactgtga
      601 cggatacgaa taatctgaat ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca
      661 ttgattatcg tgtgaatggt aatcagttga taatcacccc aaatgccaat tcaaaagaaa
      721 gtggtgtgct gacgcttaag aaatctgctg gtaccggaac acctgtcgcc tataaaaagg
      781 ctggccaaca gactttaatg gccggggcga ttgataaacc taatacctac acaatcaaaa
      841 ttgatgtgga aacggagggt tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg
      901 ttgtaccagg aacggttttc catttagact ttggaaaaac tttacctgca aaagacgtga
      961 ctactgacaa agaaggcatt gctacattgg atgagattcc tcatggtaca aaagtaacca
     1021 ttaccgaaaa atcagtaccg gcgccttata cgattgatac cacccccatg actaccacca
     1081 ttaaagcagg cgaaaccatt tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca
     1141 ttctggataa aagcggagtt gaaacaggga gtgatctttg gaatgacaat tattccttag
     1201 ctggtaatac gtttgccatt cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga
     1261 ccacggatga aaacggtcat gcggaaacac caaaagagat tgctaatgcg ttggaattgg
     1321 gaacctatta tgtgaccgaa actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg
     1381 taaaagtcga attgaagtat gccaatcaaa ccgtagcccc tgttaccagt aacgtaaaag
     1441 ggcaaaatca agaagtcact ggcgaaacca ctttgacgaa agaagacaaa gatactgggg
     1501 ataaagcaca aggtaaggca gtctttgagg gtgctgaata caccttgttt actgctaagg
     1561 atggaaaagc tgttaaatgg agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag
     1621 gttctgatga aacggtcact ttgactttag acgaaaagaa ccaagttgcc gttaaacatt
     1681 tagctatcaa cgagtattat tggcaagaaa caaaagcgcc cgaaggatat agccttgatg
     1741 aaacaaagta tcctgtatcc atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta
     1801 cccgaaatgt tacggcaaaa gaacaagtga ttcgctttgg ctttgatttc tttaaatttg
     1861 ctggatcagc tgcgggcact accgaaactg gcttcaatga tctgaccttt aaagtgtcgc
     1921 cattggaagg gaccaatgaa atcacaggtg ccgaagatga agcgaccaca gcttataacg
     1981 agcaattagg gtttgatggt tatggcaagt ttgaaaatct tccttatggg gattatttac
     2041 ttgaagaagt agaagcccca gaaggttttc aaaagattac gccattagaa atccgttcta
     2101 cttttaagga aaacaaagaa gactttgtga agagtgagta tgtctttacc attaccgaac
     2161 aagaccaaaa acaaccaatt aaaacggtga ccgttcctta cgagaagctg acgaacaaag
     2221 cattttctgt tagtttgaac cgtttaatgc tctatgattt gcctgaggaa gaagatagtt
     2281 taacttctct tgcgacttgg aaagacggaa acaaagaatt aacttctctg gattctaccg
     2341 agctagttga taaattgagt tacaatctac atgaaatcaa agaagactgg tatgtggtag
     2401 cgcaagccat tgatgtggat gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac
     2461 cagtggtgat tgcagaaaca agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga
     2521 aaattcagca taaattaacc gccgaacaag ttttaaataa aactattgtc ttattcaact
     2581 atgtgtatga aaataaggaa gcttttgaag caggcgataa gccagtagcg aaggatgtta
     2641 gcttgaataa ccaagcccaa actgtcagtt gtacagtgga acaccatgtt tctatccaaa
     2701 caaaggccca cctagaaaac ggttctcaga cctttactca tggtgatgtg gtggatatgt
     2761 ttgatgatgt gtcgattact catgatgtac tggatggctc aaaagaagct ttcgaaacaa
     2821 ttctgtatgc tttattaccg gatggtacaa acaaagaaat ttggaaatct ggcaagattg
     2881 attatgaagt aaatgataaa gaattcacca aaactgtgct tgcgaaaaaa gtagataccg
     2941 gaaaatatcc agaaggaacc aagtttactt ttgcggaaat caattacgat aaagatggga
     3001 atatcaatgg aaaacacaat gaagatttga aagaaaaatc tcaaacctta acaccaaaag
     3061 aagttccaac catactaagt acgccgaaac aaccggaaac accaactgtt ccaagtggct
     3121 ctcaagaatc tagtcccaca gtgaagacct tccctcaaac cggggagaaa aattccaatg
     3181 ttttactgtt cattggtttt actttgattt ttgcgacggc gggctattat ttctggaatc
     3241 gccggaacta aggtgatgag atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc
     3301 ctcctaaaaa agtagtcgcg ctatttttgt atccaaaact aacaatgaaa agaggaaaag
     3361 aagatggaat tgaaatttgt cgtgccagat atggctgaaa cctttggaaa gttaagttat
     3421 gccggtgaag gagaaattat aacagaagga tatggacgga atactacggt gattggtcgc
     3481 agttatcatt tgtattccag taagcaacgg gccgatgata ttgaggtggt agtagctgcg
     3541 gaagccggtg aaaaggattt tgatcaagac caaccgctaa aagccgtgaa tccccatttg
     3601 gttgccaaag gctatgagat tgagaatcgt gggtttactg attacgtgtt gtatgttgat
     3661 gatttagtga aagcatagga ggaaaagaag atgagattaa cagaaggtat tgtcgtagat
     3721 tcaggtttaa catttgggaa gttgcgtttt tccgcattac gccgagaagt acggaagcaa
     3781 aatgaggatg gaacaattgg taatgaggta aaagaacgaa cctataattt aaaatcctct
     3841 gcacaagggc gaatgattca agttagtatc ccagctaatg tacccttacg cgagtttgct
     3901 tatgatgcag aggtagagtt ggtcaatcca atcgtggata ccgttgccaa ttatgttttt
     3961 cgagaaggaa ctaccgttaa ttggttcatt aaggcggatg atttagtctt gaagcggcag
     4021 ccgaatcaag gaaatcctac gaaccaaaat gaaagaaaga aataggtgaa gattatggaa
     4081 attaatgttg aacatatttt agattgtctc gatcaatatg gaaaaggcga gctgactgag
     4141 gaacaactcg ttaaagcatt aacctctgat gaaaaaatgt ttctgattat acatcaaggt
     4201 ttacttggag aaaatagcga cagtgaagaa gactctgatg tactagactg gttaggagaa
     4261 gaagagtctt tctttatagt tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc
     4321 attttggaag aaattggtgt ggagatgccg gatgctattg aaggcttttt aaatcaattg
     4381 gttgaaacga aacaactccc ctcctcgtag cagtaaatga ctagcataac tttttgaaat
     4441 tgcctaatga aatcctattg ggctttttcg attggaggtg gaaaccatca aaatgtacaa
     4501 aggacatcgc atacgcgcag gagatcagca cttggtttat cactttgttc ttggctggct
     4561 actcgctctg ttcatcggtt ggatgagcgt cttttatttt caagagttta gacaatttga
     4621 tatttctaag ctatcacttt ctactattga aatcgtttgg tctataaaag atttagtatg
     4681 tctgcttgga agtctcgctt tttcaggagc tatgatattg ctttatattc acttttttct
     4741 tgatcattgg cgaagcttgt ggcatcgaca aaagctagct cggatgattc tagagaacca
     4801 ttggtatgaa gtgaaacaaa ctcaaagtga aggctttttc aaagatttga atagtagccg
     4861 aaccagagag actatcagtt acttccccaa aatctgttat cgaatgaaag atggattact
     4921 gtctattcgc gttcaaattt cacttgggaa ataccaagat cagcttttaa aattggaaaa
     4981 gaagttagaa agcggactgt attgtgagtt agtagaaaag gaactcaaag actcctatgt
     5041 ggagtatact ttactgtatg atatgatcgc caatcgaatt gggatagacg aagtagtggc
     5101 agaaaatggg actctgcgat tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca
     5161 tatgttaatc gctggtggca cgggtggagg gaagacctat ttcctcctca ccattatcga
     5221 agcactattg aagtcagatg cagaattgtt tatcctcgat cccaaaaatg cagatttagc
     5281 tgatttaggt acggtgatgc ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt
     5341 ggaagatttc tatgaacgta tgatggctcg tagcaaggca atgaaagaaa tgtctaacta
     5401 caaaacagga gagaattatg cctatcttgg acttccaccc aactttttaa tctttgatga
     5461 atacgtggct tatatggaga tgttaacgac gaaagaaagt gcggtgattt tgaataagct
     5521 caaacaaatc gtgatgttgg gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag
     5581 gccggatgcg aaatatttag gagacggaat ccgcgatcaa ttcaactttc gcgtggcttt
     5641 gggtcgtatg agtgaactcg gatattccat gatgtttggg gaggtcgaaa aaaacttctt
     5701 tatgaagcac atcaaaggtc gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt
     5761 ttatacgcca cttgtaccaa aagggtatga tttcttagat tcaattagta aattagaaaa
     5821 gatgaatgat tgagtgatct tcaatactga ctttatactt caagtaacgt ataatacttt
     5881 tatatagtag gaagaaaggg gataattaat atggaaagta atgattctta tttagagtat
     5941 gcaaaagcaa ataaggataa gtttatttct tatattatca aagggaaatc ccccgaagtt
     6001 ggacgagacg cagtttttat ggcagggagt cctggggctg gaaaatcaga ggttgccatt
     6061 ggattagctg agaattatga taaccatgtg attattgatg cggattactt tcgcacccag
     6121 tttcctgatt ataatggaaa aaactctagt ctctttcaaa aagcatcttc ttggttggta
     6181 gagcaggcat ttagatatgt cttagatcat ggctattctt ttattctgga tgcaactttt
     6241 gcaatattaa gtgctgaaaa aaatatctcg cgggcgttga aaaaacattt tcgagtgacg
     6301 attttctatg tttatcaaga ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt
     6361 gcagagggac gtcatgtgcc gaaaaaggca tttattaacg cttttttcaa atcaagagag
     6421 aacattgcaa aagtaaagag taaacatcca gatgtattgc ttcatatcat aataaaagat
     6481 tatacgaata atatttccga agttcattat gctacggata atctagaatt atttttacca
     6541 ctagggtata gtaggaaaga tttggaggtt caattacatg actgagaaag aacaagtaac
     6601 gaaaattgta aagaaataca ataagagcat tgcggattta tcggaaaatg ctactgcaaa
     6661 agagttcaaa acggtgatta aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt
     6721 agttggatta aataaaaaat agtaggacaa attaataatg tatgagccaa gagcaaaatg
     6781 ttcttggctt ttttctttgc aatgaatgag gtaatgtatg agatttttag atttattcgc
     6841 cggtattggt ggctttcgtt taggtatgga acaggcgagc catcattgta tcggtttttg
     6901 tgaaattgat aaatttgcta gacgtagtta taaagcaatt catgatacaa gtaaggaggt
     6961 ggaaatgcat gacatcacaa gtgtatcaga tgagtttatt caatccctcg gaccagtgga
     7021 catcctttgc ggcggatttc cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt
     7081 ttccgatact cgaggtactt tattctttga aatcgctcgg ttcaccactc ttctccgacc
     7141 taagctttta ttccttgaga acgtccgagg attgctcaat cacgaaggag gggctacgtt
     7201 cgagacgatc ctccgaacgc tggatggatt ggggtatgac gtggagtggt aggtgcttaa
     7261 ctcaaaggcc tacattcccc aaagccggga gcggatattc cttatcggac attctcgaga
     7321 cgcatgtacc gaacaagtat ttcctatcat tggaccgtct tcaacatctg atcaaaacat
     7381 cagaaatttg gtaaatatca atccttccaa tcgaggaatg ggtggacaag tctatggttc
     7441 agatggcgta gcgccaacct taacaggtga tgaaggaatc aaaatcgcac tgccagcaag
     7501 ggatggaatc tctgtagcag gactattacc gggaaagttt gaacaaggta atcgagttta
     7561 tgaagttact ggaaccgccc ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat
     7621 tcgaaaaaat gaaacagaat atcaagaggt gaagccagaa gacagtgtga atctggcttt
     7681 tcctaattcc acaagtcgcc gaggtcgtct gggaaaacag agtgttcata ccttactaac
     7741 aggagaccaa caagcgatcg tcacggatca gtatcaaatt cggaagctaa cacctagaga
     7801 gtgttggcga cttcagggct ttcccgattg ggcttttgat cgagattctc aagtcaattc
     7861 agatagtcag ttgtataagc aagccggaaa ctctgtaaca gttccggtca tttttgatat
     7921 tgccagaaga ttaaaagagt tggatgtaca tggtttatga tgagttgatt ggagaaattt
     7981 attgggtgat tggaaagatt cagtcagacc cagaattaga agaacaattg cgacggttaa
     8041 aatttgatat tcgcaagaat ggtgtcaaag tcgctggcga tccatattta atgaatgagg
     8101 agacggatgc tcgaattgaa ataaatcaag taattgcaga atttgagcgg attgcggacc
     8161 aagcaaaaga accagatatt cgtcaatact tgttagaaat gaaagcagag ttggaagtaa
     8221 atggtattac agatgagtaa cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga
     8281 ggattcgcga agcagaaatc ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga
     8341 ccatgatgtc tcacccccgt atctaacagg ggggtacaaa aacacaaaaa ataagtaagc
     8401 aaccattaag aatgcttgtt gtatcaaaga tagtaagtaa tttgtatagg tgttaactca
     8461 tttgaaaaag ttaacacctt gaatttggag gtgagaactt ggcacaacgg aatttagatt
     8521 atcggctatt aaaagaccga cgcaacgaat acggcgtttc acaaaataaa ttggctacgg
     8581 cttgtggact tagtcggccg tacttaaatc agatagaaaa tggtggcgta accgcatcta
     8641 taaaaaccat gaggaaaatt tttgaccaac tggaaagctt taatcccgat ttgcctttga
     8701 cgttactgtt tgactatgta aggattcgct ttcccacaac ggatgcacga aaaattattc
     8761 aagaaattct ccatttgaaa tttgagtata tgctccatga agattatgcc ttttactcct
     8821 atcaagaaca gtatgtcatg ggtgatattg tggtgatgtt gtctcatgaa gaagataaag
     8881 gtgtgctttt agaattaaag gggcgaggtt gccgacaatt tgaaactttt ttactcgctc
     8941 aaaagcgtag ctggtacgac tttttcgagg attgtctaaa ggccggtggc gtgatgaaac
     9001 gcttggactt ggcaatcaat gatcgagtgg ggctattgga tatacctgag ctaacgaaaa
     9061 aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt taaaagttat cgttccgggg
     9121 aacttttaaa ggctgatgaa aaggacggca tgggcaatac cttatatatc ggcagtctca
     9181 agagtgaggt ttatttctgt ttgtatgaaa aagactatga acaatatatc aagttaggga
     9241 ttccattaga ccaaactgag acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg
     9301 cttttcatgc gattcaagat ttgttaaaag gtcgtagcat tgagagcacc acgttttcca
     9361 ttatcaatcg ttacttgcga tttgcggata aagtagaagg taaaaggcga accaactggc
     9421 ccttgaatga acaatggggg cgttttattg gacggaatcg gaaagagatt caattaactt
     9481 ccgagccaaa accgtatacc attgaacgaa ccttgaattg gctaggccga caagtcgccc
     9541 ctacgtggaa aatggctaag gagttggatc gattgaatcg gacaacctat atccaggata
     9601 tggtacagaa tgcacgctta tcagaccgac ataaaaagat tttggagcaa caaagtatgg
     9661 caattgaaaa tttgattata tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt
     9721 tttggtattt ggtggcagta tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc
     9781 agcagcggat cgagcaatgg aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt
     9841 t
//
LOCUS       DAZUKR010000100         9809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000100 DAZUKR010000000
VERSION     DAZUKR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.102"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(571..957)
                     /locus_tag="ACG89C_002125"
     CDS             complement(571..957)
                     /locus_tag="ACG89C_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4900648.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(954..1475)
                     /locus_tag="ACG89C_002126"
     CDS             complement(954..1475)
                     /locus_tag="ACG89C_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4900649.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(1477..2511)
                     /gene="sppA"
                     /locus_tag="ACG89C_002127"
     CDS             complement(1477..2511)
                     /gene="sppA"
                     /locus_tag="ACG89C_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4900650.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(2544..3959)
                     /locus_tag="ACG89C_002128"
     CDS             complement(2544..3959)
                     /locus_tag="ACG89C_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4900651.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3928..6081)
                     /locus_tag="ACG89C_002129"
     CDS             complement(3928..6081)
                     /locus_tag="ACG89C_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS4900652.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            6339..6575
                     /locus_tag="ACG89C_002130"
     CDS             6339..6575
                     /locus_tag="ACG89C_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS4900653.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            6594..6809
                     /locus_tag="ACG89C_002131"
     CDS             6594..6809
                     /locus_tag="ACG89C_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS4900654.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(7512..8264)
                     /locus_tag="ACG89C_002132"
     CDS             complement(7512..8264)
                     /locus_tag="ACG89C_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4900655.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(8279..9526)
                     /locus_tag="ACG89C_002133"
     CDS             complement(8279..9526)
                     /locus_tag="ACG89C_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4900656.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(9602..9748)
                     /locus_tag="ACG89C_002134"
     CDS             complement(9602..9748)
                     /locus_tag="ACG89C_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFS4900657.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         3212 a         1732 c         1378 g         3487 t
ORIGIN      
        1 atattaattt tatttaaaaa ttgacattaa tgactcttaa tcagcgggtc gcgggttcga
       61 gcccctcacg gcccattggg tgccaaaccc acggaacagg taaatgttgg tgcgaaagca
      121 tttcgaaaac atttacgctg ttcttttttt atttttcaaa ataactaggg ctttttattt
      181 ttaactcaaa gtccactgta gccctttgga ctttgagtta aaaaagaagc ataattatga
      241 acagtaaagt ttctaatata acaataattc gagaagcttt gcaaaatcat gctgccaatg
      301 aactctctaa acatatagga ttaaatctaa gtactataaa aggttaaaaa gtgaagaata
      361 tttaatagaa aaactaaatt tacttgctgc tttccccttt tccattaata caacaatcag
      421 agagtgtctt tttttcgaaa aaattagaat aaataaaaga cacgtagttt ttttatctac
      481 atgtcttcca tcgactacaa gttatcattt ctcaaaacgt atttagcttt taattgatta
      541 atcgtttcac gttgtctaca cattgcctaa ttaagatttt cttattagaa gcatcaatcc
      601 cgcaataata cctaaaactc caggaattac ccacaataat gttaaaagaa aaaatggaat
      661 aatagataaa atgagtaaac taataccata gaccactgtt ttttgatttt ttcttactaa
      721 aaaacctatt atgaaaatca ttatcgaaaa aactagggta attcctccaa ataaaacaac
      781 attcccgctt agattgacta acatatctat atccttactg gttagttctg tagtatttaa
      841 agtagttgaa tcaatagaag atagaaacat accacccatc aatgctaaaa gactacataa
      901 aatccctgtt attccactaa ttaaaatcaa aataaactct gtttttctct tattcattta
      961 attctctcca gattaagtgt tttagaatta gaaatttttt gaccatagtt aatagcttct
     1021 aatgaacgaa taacaaccac tgacgtgtta gaaaaataat ccccaatatg ttgtttcttg
     1081 ggcgtgaaag cagccaccaa atacccaatt tgaaatacaa aattttttaa tataaaccta
     1141 catatacact cacgaataaa gatagtttgc caacttagct ctttttcctc aaagctcact
     1201 actcttatcc caaaaatcat tttcccaatt gtttgtcctg aattcagctt agtcaaaagt
     1261 acaaaatagg ttaaataaat agctaatgac gctataccat aaaaagtaat aatggatttg
     1321 tcagattcca gtcccatgat tttgtaaaag agacctatag tagcatgggt gatgatgcta
     1381 atacaaatta aatcaataag aaaagcaaaa aatcgtaacc aaaatcccgc aaatacataa
     1441 ttaggcaaag tttcaatgcc actcttctta ttcattttat agccctccat agtaatacat
     1501 tggttttgga gtatcagaaa atccaagttt ttcaaaaact gaaattacac gatctgatgt
     1561 agttgccttt agcccttgcc attcagcaat cttattacct aaccaagaag aagcaaagcc
     1621 agttgtgtca tttttatatt ctataaccgt cgcatcagtt aatttcttct ctttttttag
     1681 ggaatctaat gcatcttcag agtaaccaat ttgatccacc aacccatttt ctactgcttg
     1741 gttaccatca taaattcttc catccgcaag ttctttcact gcattagtat ccatatttct
     1801 tccttttgct acaacattga caaaacgatc ataagcactc atcacaaatt cctgtataac
     1861 tttattctct tcctctgaag gtttgtgttg aggtgacatc atatctttta atgcaccact
     1921 tttatacgtc gcatcagtta cgccaagttt ttctaataag ccagaataat ttaaaccaga
     1981 aataatgaca ccaattgatc cagttgttgt ttcttccgta gcaaatattt tatctgaacc
     2041 agcagaaatg tagtaccctc cacttgccgc tgtattttta aatgcagtat agataggaat
     2101 atctagcttt ttaatttttg ccatttcttt agcaatctca gcactttcat atattcctcc
     2161 cccaggtgaa tttacttcta ataacactcc cttaaccgct ttgtcttctt gaattttctt
     2221 tagctgtgtt aaaaaattct gatggttata ttgttcccta ctaaaaagat tgctttcacc
     2281 tgtatctgca ataactccat tgacggatag tttgacaatc tttttatttg atgcgccttc
     2341 ttctaacgtt tcttctgtga tttcgttaga tccgtaaaaa atcttattta ttcctgttag
     2401 ggttgtattt gatgcttcct ctttatcttt tcctggaatt agcgcgacga taatggatag
     2461 taacaataat ccgcacgcta ctcccacagc aatccatctt cttttattca tataaactcc
     2521 tcctatattc ttagtaaaaa taattatttt ttatttagaa acacatcgac aatttgttgc
     2581 caatatgttt tttctcctac aattaaagac aactctccag ttaatccata acgaaacgga
     2641 aacgattgag ttgtttttaa cgttcctttc actgcataaa aaacaccttt ctcagtggca
     2701 gtagaatttt ctgaaatttc atctaaagtt ccgtcgatcg taatcggagc cccacctttt
     2761 tgatccaatt taaaatgaac cttcatccct tctttaatat aggtactttc attcgcaggt
     2821 aaaagtgctg taaatttcat ttttgatttt tcgtggatcg gataaatttc tgcaagcact
     2881 gttcctttgg ctagttctgc ttgtcccttt gtttcttcat ttaaatgaac cgttccagat
     2941 acaggagctt ttagtattcc ttttgaaagt tgatcgtcca aacttttgat aactgactgg
     3001 ttcttctctt gtttttcatt tagctctgta actgtttgtt ttacagttgt tattgcttgt
     3061 tctagctctt gctttccttt ttccgtttga ctaattctgt cattctcaga agtagttggt
     3121 ttaactaatt tagctttttc tgactgatac tgttctactt ctttttttag ttgctcaatt
     3181 tgttcattta tgcttgctga aattgtcagt ttcacttgat ttttttgatc atcagaaaca
     3241 ttatttagtt gttcttgcca attctcatag tttgccatca tttcttttga aaaatctttt
     3301 aatgattgat tattactcca agctacctgt acttgttgcc attcttgtaa tttactatct
     3361 gaactctcaa tttgtttttc tagttgtctt tttgtctttt cgtatacttc taattgcttt
     3421 tgatcattta attcactttg cttcaaagcg tagcggagtg attctttttc tgacaacatg
     3481 ctctttaatt gattactgta gccaaaactg tcatccgttg aaaacaaatt ttttccttta
     3541 cttatacttt caataaatag ctgagccatc tttatttgtt gagtaatacg ttggttttct
     3601 tgttctatct gagccttttc attctctatt aaagaacaat ccaatctaac caacacatcg
     3661 tccttatgaa caaattgatt ttcttttagg aagttttcca ttattttagc ttctaatggc
     3721 acttgtaact tctctatttt ttcggggttc aattgagccg ttgtccgaat aacaacctct
     3781 tttttagcaa aggttaaaaa caatccaata atacataaaa aaagaattac agggtataac
     3841 acccaacggt agaagctatg atgttgttta gtatagatac tagaatgttc gaaccaatta
     3901 ttattctgca taaattgttt ccctctccta gatttcccat aatttttgat agataccatc
     3961 ggcaaaacga agttcatcgt gcgttccttt ttctaccaaa tgcccttcat ggagaactaa
     4021 aatttgatca catgcttttg ctattggtaa atgatgcgca ataaaaataa tcgttttttc
     4081 ttttaaattg aggagataat tcataattgc atgttctaac aaggtatcta atccactagt
     4141 tgcttcatct aaaattaata tgtccggatt ttttaataaa gcccgtgcta aggctaatcg
     4201 ttgtcgttgc cctcctgaaa gattatttcc accttcttcc aacaatgtct cgtaacgtaa
     4261 tggctgttga ttaataaatt catttagttg gactatctca caaacttcta ttatttgttc
     4321 gaaacttggt gggctatcta aaccgaatat aagattctca atgattgttc cactaaagaa
     4381 aaatgactct tgaggcaaat atgtcactcg atcgcgtaat atctgaaaag gaatgtctaa
     4441 gtgattaatg tttccataat ggatcgatcc ttctgatgct tcataaaaac gaacaaataa
     4501 ctttgcaaga gttgattttc cagaaccact aactccaact atagccgttt tactgtgcga
     4561 aggaattgta catgaaattt cgttcaacgt tggggccttc atgctatacg agaatgaaac
     4621 gttttttaca gtgatatctt gttgaaataa ttgttcagaa agcatatatt caaaagactg
     4681 gttcgatttt tctgaatcaa tcgcgaaaat ttcatttaaa cgattatttg caacttgagc
     4741 cgtttgcatt ttcacttgta aattaataat gttttgtaaa ggatctgtca aaaatactag
     4801 taaagcattg tatgtgataa gttgacctag actaattcta ttatccataa caaaataaga
     4861 acctagccat aaaattaatg cactgctaat taattgaatc aaatgtttaa ttccttgttg
     4921 aatattatca agcgtaaccg tacgaaatga ttttttcatt aatttaacaa attcatggtc
     4981 tatacggtta tatactttct cttcaccgct atatgcttta attgtttcta ttcctttaag
     5041 actttctatg atactagaat ttacagctgc accggctgcc atttcttccc tgttcgattt
     5101 ttcatagctt ttcacgaaag cgaatattgc aactacataa aaaggcaaag agcatagagt
     5161 gattaaaaac aacgtactat tctgaatagc taacgtaacc ccaacaagca acaccatacc
     5221 aatatctaaa aatattgata acgtagcact tgctaaagca tcaataatct tactggcatc
     5281 aagaaaacga gaaataattt cccctgattt tctcgtagca aaaaaattca ttggtaaagt
     5341 taacacatgt ttgaaatact gaagcataat tgccatactc attcgctgtc ctaaaataat
     5401 aagaagataa ctacgactat attcaaacaa tacccgaaat aaatagacag cgatcaagcc
     5461 aatggaaatt atatttaaag ttgaacgtgc ttgattaggg atgaaaaaat caagaatacc
     5521 ttgaaaataa tatgagcttc ctatgccaaa aaacgtaatg aaaatcgatg ctaaaacgat
     5581 gtggaatact aatccttttt gtttccaaat tataggcaaa aaagaactta atccagctac
     5641 tttttcttta ataggaatat aagtttctct aggcataggt aataaaagaa tacctgtcca
     5701 ttctttttga aacgcttcaa tcgtttttct attttttcca ttagcgggat ctgcaatcca
     5761 tagtacgttt cccttcgttt tataaattac aacgtagtgc atatagctgt tatcaaccaa
     5821 tatatgtgca attaatggta agataagatc tctttctttc cagatctcac tatctgcttg
     5881 aatagccata caatcaaaag acaatttttc aaatgtcttt ttcaatccaa atgctgaagt
     5941 accctctaaa tcagtcccag ataggtcccg taatttatga atcgggatat cagttttaaa
     6001 atattttaaa atcatcgaaa ggcaagcaac accacaatct ttttcatctt gttgctgcac
     6061 aaaaggaaat gattttatca cttatctaac acaactcctt ctaaaaaatt tttatagaaa
     6121 aattctcaat atagctttaa tacagtgaaa atattgtctc ataattccac aaaaactttg
     6181 aaaacagttc ttgaacacta actttttcat tcctgaatca aatttatttt ggaaatttta
     6241 ctattcaaga ataaacttga taagtttcag gaagattttt actgttaatc aaatacgtat
     6301 gttacagtag caccgtaaaa aatattattt aaagaagcgt gtttaaacat gcaaaatgta
     6361 aaggaactaa gtattgtgga aatgcacaaa ctgttggtgg aggcaataat gcttggggaa
     6421 aattggggca agttgtaggt ggacttacca caggggcagt aggtggtgca ggtttaggaa
     6481 cagcaatttg cggaccagct tgcggtgttg ttggagggtt atatggagct gtcgctggag
     6541 gtgctgcagc tggctgggat gctcgaaaaa aataaaaaag gagaataagt agtatgcata
     6601 ttaaaaacac aaaaacaaca tttatattat cttcagaaga gttgaaaaat attcaaggtg
     6661 ggagtgccgt gggagtttta ggcactactt tttctggggc aacagcagga gtaaaacttt
     6721 gttcagccgg aggaccttat gcaatagctg cttgtggtgt aggtggagct ttattaggtg
     6781 ctggattcag catgtggacc ggtgcgtaaa caattaaaaa atatcaaacc tcaagattgg
     6841 tttgtgtcga ttacgaacat aggtatgttc tttatctttt caaaagtttg gaatggacag
     6901 aactttttac taaagcttct tgtgctagtt tttataatta tattaaattt ttatctttta
     6961 ttaaagtcag ataaaaattc tttggaaatg taatggtaga gatattttta caaaaggact
     7021 tgcagcgctg tagttggtgc tgcaggagga cctatttcgg ccgttacagg aggtattggt
     7081 aatgtacttg tcggatgtgc attaaagcct gttcctgcaa gttaaattta taaaaacagg
     7141 aaagtaatta tctcaaatcc tatcaagtac aatcatagga ttgcacttga caggacaaat
     7201 tttttaacta aaaaatgaaa aacactttct aaaaattagt agaatttgcc aaatctttcg
     7261 catcttccac catacattta attttaattc ccctttatgg tacttaataa aaacaagaaa
     7321 agaactattc atttggccct tttttggttc cagaccatag aaatattcct ccagtaacta
     7381 atattgctgt tatgcacgta actaataaca gctataaaaa ttggtacaac tttaatagga
     7441 ttcagaaaaa tttgcactag tgaaggaatc tattttatta gaaaaatgca atctccctaa
     7501 agaaaaaacc actaaacagt gctttctttc aacttctcct taattttttg tgctttccct
     7561 aaagtaaagt ttctggccaa ctctgaatca aaatatagtg aacgtttttt aaaatctatt
     7621 tctttaacgt taaagatatt tactaaacac gctcgactaa ttcttgttaa cataggatat
     7681 ttcttttcaa gctcactaat tcttccataa aattcgtact gcccatcttt agtacacaat
     7741 gataatctat gtggtaattt tgaagcttct acaaaataaa tgtcatttat atcaaaagta
     7801 aatgtttgag aacctatgga aaaaacaaat gcttgattcg taacctgttt tgttgcatct
     7861 atcctttctt gcgctaataa caataactca acaatctcgt ttcgatactc atctagtgtt
     7921 tgatcttttg ttacaaatcc taacgtttca actcttcttt ttattgttac gggtaacatc
     7981 tcatcatgag tagttgtaaa aatgattttt gcttgaacat catattttct gattacttct
     8041 gctaattcta taccgttaac ttcatgattt aaatcaatat ctaagaaata tattccttgc
     8101 tttggtcgga attgtttgag gtatttttta acttctaacg gactttgtgt tttcaatact
     8161 atcttaaata ctttatcgtg aaaaagaatg aaattatcaa taattcgttc taactgattt
     8221 aattgtataa tttgatcttc acataatatt attggataac tcataaatcc tccttccctc
     8281 aaaacaacaa aataacttga gtagtaaatt gatacggatc gttttgatag ttcacaaaca
     8341 tattgggatt ctttttattg atttcctcta tactagataa acctaaccct tgatgtccct
     8401 tttttgttgt tattcctttt ttcattagag tatctataga gagtagtatc tctctacaac
     8461 tattcgatat cgaaatttct atctgtttat catctcggta aatagctaga gacagttctc
     8521 tatcttgact ctctatagat gcttcaattg cattatcgag aataattcca agcaccctaa
     8581 tacagtcaaa tattggaaca ggaacttcaa taatagtact tggacattcg aatttacaac
     8641 aaatcttatt gttatttgct tggtatagct tcgctatcaa caaacttttc aaatattcat
     8701 tttttatatt ttttaaataa cgatattcaa acttattggt tagataagaa ttagaataga
     8761 tctctaattc ttttatttct ttttccaata tcgaatcatg gttttgatta gcgatttctt
     8821 ttaaactcag caataaattt ttatagtcat gtctaaatct ggcaaatttt tcttgatctt
     8881 tttccagtga atcagtatat ttttttagat aaaccaattc ttttcttttc aattgctgtt
     8941 catattgttc tcgttgccta atagttattc taataaataa aaatagaaca acaaacattt
     9001 gaatgattaa aaatattaag acaccaaaca caaaatgatc aaatacttga tattgatgag
     9061 caacataaga aacaaataaa ccaattgtaa gtaaataaat catgaatata gcagtaagtt
     9121 tagaactgga ctgttcaaca atcctattta tagctaattt tctatataac caaataaaca
     9181 tcagcaaaaa caaagaatca agcattatct ggaatactac ataaccatac ccttttatcc
     9241 cttcaaatga aaacatatac gtcataacca tggaagtaat aatagatctg aaatcatcaa
     9301 taagcataca taatagtaat ttatttaaaa gcactatatt agttttcttc ttttccttta
     9361 atataaaata acttccgatt ataggtatag ctgcggaaac atcagtaaat aattcaacaa
     9421 aaaaagtgag tggtatcaat aaacatacaa aaagatattt tcttttgtta agatcagtat
     9481 tagtcatata aaataagaga aaaaagaaaa agtttcccac gaacattctt aatagataag
     9541 cttttgtatc gatagtaatc aagagatccc ctcctgattt taggatatca taaaaaaaat
     9601 tttagttaca ctttttaaat aatgaaaaaa cacattccac taaatttgat gccggttttc
     9661 cttgaggttt tgttccagca cctcctttaa tttttttaag ctcttgatct gaacattgtt
     9721 tagcatttag tctatttttt tcttccatta cgattccccc tacaaactat aaatactttt
     9781 atgtcactga ttcaatatac ctatttttc
//
LOCUS       DAZUKR010000101         9591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000101 DAZUKR010000000
VERSION     DAZUKR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.103"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..2095
                     /locus_tag="ACG89C_002135"
     CDS             167..2095
                     /locus_tag="ACG89C_002135"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4900658.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2233..2688
                     /locus_tag="ACG89C_002136"
     CDS             2233..2688
                     /locus_tag="ACG89C_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4900659.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2768..2896
                     /locus_tag="ACG89C_002137"
     CDS             2768..2896
                     /locus_tag="ACG89C_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900660.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3158..3427
                     /locus_tag="ACG89C_002138"
     CDS             3158..3427
                     /locus_tag="ACG89C_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900661.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3827..4081
                     /gene="rpsT"
                     /locus_tag="ACG89C_002139"
     CDS             3827..4081
                     /gene="rpsT"
                     /locus_tag="ACG89C_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4900662.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4170..5216)
                     /gene="holA"
                     /locus_tag="ACG89C_002140"
     CDS             complement(4170..5216)
                     /gene="holA"
                     /locus_tag="ACG89C_002140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4900663.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5254..7527)
                     /locus_tag="ACG89C_002141"
     CDS             complement(5254..7527)
                     /locus_tag="ACG89C_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS4900664.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7520..8059)
                     /locus_tag="ACG89C_002142"
     CDS             complement(7520..8059)
                     /locus_tag="ACG89C_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS4900665.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8195..8413)
                     /locus_tag="ACG89C_002143"
     CDS             complement(8195..8413)
                     /locus_tag="ACG89C_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4900666.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8410..8655)
                     /locus_tag="ACG89C_002144"
     CDS             complement(8410..8655)
                     /locus_tag="ACG89C_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4900667.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8771..9499)
                     /locus_tag="ACG89C_002145"
     CDS             complement(8771..9499)
                     /locus_tag="ACG89C_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4900668.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2967 a         1863 c         1663 g         3098 t
ORIGIN      
        1 aatgttctgg ttttttattt tctttaataa taatcattat aaataataaa aacaacatca
       61 agcttaatca ccaaatgata accattatcg ttctcattta gctgttatta ttatcaatta
      121 catttttatt atgctatcct tgttttgaca aggaggtcta ataacgatga ctaatcagaa
      181 aaaagctttt gcagcaacaa tcggctgcct tttatttatg atcttcggtt ttatcttcga
      241 ccgattctct cttaccatca ccccactttt ttatatcagt gccattatca tcggtggata
      301 taaacaaaca tcggaaggct tgaaagaatt gtggaatgat cggacactga atgtggattt
      361 attgatggct ttagctgcta ttggtgcctg tttgatcggt aattactttg aaggagcaat
      421 gttgacattc atcttttgtt taagcggtgc attagaggaa tacacaacta ataaaagtca
      481 aaaagaaatt actgctttga tgaatcttca accacaaaaa gcccaattat taaaagaaaa
      541 tggacaaatg caagaagtag atgtttctca actaaaggtg gataacttgg tatttgtcgc
      601 taaaggagca gctgttccga tagatggctt tttagaaagc acacaggcaa cgatcgatga
      661 gtctgcatta agcggtgaat ctgtgcctgt agaaaaatat ttaggcgatg agatgtatgc
      721 aggaacgctg aatcaaggaa atcccatcac gattcgagta acaaaaacga gttccgaaac
      781 tgtctttgct aagattatcc aattagtaga agaagcgcag aatacgccaa ctcaaacagc
      841 ttcatttatc gaacgaatcg aaaataatta tgtaaaattg attttgcttg ctgttcctct
      901 aatgatcctt ctcccccact ttttcctagg ttggagttgg gatgaaagct tttatcgagg
      961 aatggtatta ttagttgtgg cttctccttg tgcgcttgtc gcatctgcta caccagcttc
     1021 tctagctgct ctttcaaatt ctgcaaaaaa tgggatttta atcaaaggcg gtatccattt
     1081 agaaaagctt tctgaattaa aagcgattgc ctttgataaa acaggaacgc tcactaaagg
     1141 aaaaccagtt gtaactgaca gctttttctt tgatgaaaag gatttggcac aacaacttct
     1201 tgttgctatg gaacaaaaaa cgacgcatcc acttgctcat gcgattttgc aatatttcga
     1261 ttgcacaata cctgaagaaa tccaatactt accaatagaa gaaattactg gttttggatt
     1321 acaaactacc tatttaggtg cgcaatggaa agttggcaag cacgcatatg atcctgagac
     1381 gatgataatc agcaaagaga ttgcggaaat gatcgaacgt ttggaaaatc aaggcaaaac
     1441 cgtgatttat ctttctaaag atcagcagtt aattgctgtt ttaggtctgc tagatattcc
     1501 aaaagcaaat acccaacaag taatttccta ctttaaatcc caaaacatcc atacaagtat
     1561 gataaccggc gaccattctg gcacagcaaa ggccattgct gaacaagtag ggattgaaca
     1621 ttactatgca agctgtactc cggaagaaaa aacacaacta gtcaaaaaag agaatgaact
     1681 ttaccaagta aatgcgatgg ttggtgatgg agtcaatgat gcgcctgctt tagcagccgc
     1741 ttctttaggt gtcgctatgg ggcaagggac agatgccgct atggatatcg cagacatcgt
     1801 cttaatgaaa agcgacttaa ataaatttgt ctacagtcat cggctatctt taaaaatgcg
     1861 gcggatcatc aaacaaaata ttattttttc gattcttgtt attctcttgt tgattgcttc
     1921 taacttcctg caatttttaa atctacctct tggagttgta ggacacgaag gaagcacaat
     1981 attggttatt ctgaatggct tgcgtttact aagaccttta cctgatcttt ctgattcttc
     2041 aaaaaaatgt aagtcttgtc cactatacaa agcgcggctg tcttctgtca aataagcaaa
     2101 gtgttttttt atgattttgt ttattttatg aaaaaaacag cttatgaata ggtcttgaag
     2161 gatgaattcc tacttttgta actttctaaa aacatgttat gattgtttcg aagaaagtga
     2221 ggaatatatt tcatggaaag ttggcttttt ttaggaatta ttttacttgt agcaatttta
     2281 gctaaaaatc aaagtctgat gatagcggca gcttttgttt tgattatgaa gctcatacca
     2341 gcctcccgtt cctttttccc ttggcttcaa ggcaaaggaa tcaatattgg ggtgacgatc
     2401 atcacagcgg ctattctaat ccctatcgca acaggagaaa tcggtctacg tgatctatta
     2461 gaagccttta aatcccctat tggctgggta gctgtttttt gcggtgggct agtggctgtc
     2521 ttatcttcca agggtgtagg attgattgct tctgatccac aaatcactgt tgcacttgtt
     2581 ttcggcacga ttttaggtgt tgtcttctta agaggaatcg ccgccggacc ggtcattgct
     2641 tctggtatca cttactgtat cttacaagta tttgctttag gaaaatagaa caaattttgt
     2701 ctattttagg agattcttaa cacccccaat ttactgatcc gtgatatact gacaacgagg
     2761 tgatacaatg ttcgaaaaat taaagtattt cataggaaca atcggctggt ttcttctagc
     2821 ggtacttttg attctgggct tagcttatct attatttcct gatttgatac aaaatgtgtt
     2881 tgatactgct ttttgattga cctattaaaa cgatataggg gatgctcctg cgatgcaggg
     2941 gcttttttta atcaatgttt tgtaataatt taggccgtgc caatgagact tcctatagaa
     3001 ttcgatgctc ttttttcttt ttggctcttc tgctcttttt tgttatacta gattacagtg
     3061 gacaaaagaa agaatgatcg catccacttc aacgaatatc aaaatggaag aattagtgcc
     3121 tgttttcagg ttactttgaa aagaaaggaa gacgcttgtg gaaacttttg aaggtctttt
     3181 aattgatacg tacgttttac aagaacagcc aatgatgctg actgggatca acttgcagct
     3241 tgaggaccgt gtgatcaatg gcctgcttaa agatcgctat ttggcaaaat atatcttacg
     3301 tttgccccta aataaatatg tgctggaagt aagtggcaaa tggaaccgtc ggaaccagtt
     3361 ggtcataaaa aaaatgttga taaaaaatat cgatgaatac attaaaataa tcggtacacc
     3421 agaatagcct ttactgatca aaggatactg gttataaaat aactgcagtt tcttaataga
     3481 aaaacaaaga acaacaggga atcgtatccc ttttcaaaaa aaactttgca aacggttctc
     3541 tttgttatat aataacttag taccaaacat tcccattcaa actttgttta aatagataaa
     3601 taaaaatcta gtttggtttg acgctattgc ctcccacaat agcgtctttt tgattttttt
     3661 cagcttgtac tatccgtttt tactgaaaaa aaatcctaat aaaagactgt taaggaaaaa
     3721 aacttgtcat gttcatattg caaagcacgc tgcctttagg tataataatg aatgttgaat
     3781 tatgcggtaa cacgcgtaag aacttaagtt atcggaggtg aaactaatgc caaacattga
     3841 atctgcaatc aaacgtgttc gtacaagcga aaacgctaat gtgaaaaatt catctcaaac
     3901 aagcgctatg cgtactgcaa tcaaaaaatt tgaagatgct gtagcttcag gtgctgacaa
     3961 cgtagatgcg ttatataaag aagctgttaa agctattgat atggctgaat caaaaggtct
     4021 aatccacaaa aacaaagcta accgcgacaa atctcgttta agcaaaaaaa tcgctaaata
     4081 aaagaatcgg cttcggccga tttttttatt tgtcaaaaaa aggacaaaaa caaacgacct
     4141 tcccgcatgc tttgccctcg tctttcttct tatcgatttc ttaacaactg ccctgtgcgc
     4201 aaaacgaata gttgaaaact taattctttt tcgatttgtc ccgttttgat ttgataatcc
     4261 atctcaacta attgatcaaa caactgtacc agcaaatgtt catcgaattt ttttacttcc
     4321 tgcatggcaa gttttacacg atatggatgg attttcaatg tttcagcaat atttgcctgc
     4381 tgataaccaa tctttacaag aaattttgtc tgtaaaaata aacgtaattg agaaagcaaa
     4441 atcgcattaa ttttgattgt ttcttcccct tgcataacta aatcttcgta taaacgcaaa
     4501 gcttgttccg tcttcccttt taaaatatat tgggtcatgt caaaaatatt atgttctaac
     4561 gttttaggaa ctaattcttc tacttctctt accgtgattt gctgttgatt ttgtccataa
     4621 aggaccaatt tctgtaactc tctcatcatt ttggataaat ctaagtcggt caacctcaag
     4681 aataaatcaa ttgcacttcg ttccattttg attgaggaat tctctaaggt attcatcaga
     4741 tattgacgta cttctttttc attcaactgt tggacattga tgataattgc tgtttttttc
     4801 aattgtttcg ttactttttt ccgttcatcc aacttttcat atggcgcaaa gaatactaat
     4861 acagtcgatt ctaatggttc tttcaaataa gccgtcaata aatcagtatt ttgttcgatt
     4921 ccattgttta ctttttcacc tgttaaaaaa taaggattct ctacgaatac taaccgttga
     4981 tctccgaaaa acggcaacgt ctctgcttca gctactacct catctaaact tgcattttcc
     5041 atgtcaaaac ttaaaaaatt taagtcatct tcgccctcta attggatttt tttgacaatt
     5101 tctgtccgaa cttgatcttg gagaaacttt tctgttccta aaatcaagta acaagatgca
     5161 agtttttctt tacgtatttt ttgtaactct gcttgtatac tcaaattact caccactttc
     5221 aaaaattatc ctaccatgag cagatgagaa ttactagtct atcattgttt ccactagccc
     5281 atgaggatga aaaactggat gccagcgata ggtgatcatt ccttgttgat ccgttcgcca
     5341 tgtttttatc tgattttctt ctagtgtctc caatacttcg ctgtgcgggt gaccataacg
     5401 attatttacg ccgcaggata taatcccatg ttttgcttca atatgggaaa gaaaatctgt
     5461 gttacttgac gtacggctac cgtgatgacc taatttgact acatcggctt tcagattagg
     5521 ataaatcaaa agcaattgtt tttccattgc ttgattcaaa tctcctaaaa ataaaaagcg
     5581 cctattgtta tattccatcc atagaccaat cgaatcttca tttcttccct ctcccttttc
     5641 aaaaggagtc aatacataaa gatcataata accgctgatc ctgtccccat gtttgatttc
     5701 aatgatctca gtaccatttt tttgagcttc agataaatgc ctttgtaatg gctcatttcg
     5761 tccagcgccc caaccgatat aaattttttt gatagtaaaa tgctgagata acgtgggtaa
     5821 ttctcccaca tgatccgtat cgtcgtgtgt caagatgagt ttatcgatta cacttatccc
     5881 ctgacctttt agatatggaa cgatatttct ttcagcagga ggacgtatca taccttgcgc
     5941 ccacttttct ctttcaaagt gtaactgacc tccagtatcg atcaaaatcg tttcttttcg
     6001 aaaaggactt tgcaaaaaga tactgtctcc ttgaccgaca tcgataaaag cgatcgttga
     6061 agaaaagcgt aaaaatggca aagtcaaaaa aataaaagaa aaaagaccaa cataaaacaa
     6121 gtgtttcaca ggttgttctt tcaacctatc catacaaatc agtaatccga aaatatacat
     6181 cccagatagc acaaggggca aacgtcctat aatgagagac gaccctgaag aaaaatcgat
     6241 tattcgctca aataagatta atccctcttc tatcagcaaa agtaaagttt ccggcagcca
     6301 gcgattgaag agtgtgagaa ataacacacc aggaagaaaa agaaacttga ataccggtgc
     6361 aatcagtaat gtaaaaagac tacctaaaat aggccactca taaaaatgcc atacaatcaa
     6421 cggcatcgtg atcatagtaa acaggacgtg agaggccgtt tttttcaacc aatctccatc
     6481 aaaagaaatc aggataaaca aaaaactcat gaaaaaacta agctgtccac ccgcatgaaa
     6541 aagaaatcga ggatcaaata gtaaaagaag ccaaaagaca actgcaaata agtccatggt
     6601 ggaataatgc ttattcagca atttgactag caatcgcaat aaaaacagca aactagcacg
     6661 caaaacagaa atagaacttc ctgcaataga aacataacag atagttagta gactcaacgg
     6721 aataatactg acgtgtagtg gccatccgat acgtcgaaat aaataataca gccaccctaa
     6781 ataaaattgg atgtgtagac ccgatagact gaacagatgc agcaaaccac tctccctaaa
     6841 aatttcttct ggttctctga atcctgcatc tttatagccc cataataaag aattaatata
     6901 tacacttgtt ttttgcggaa attcagattg gacaaaggag ataaaagatc ctctttttct
     6961 ctgaagccag tttcgccagt gattttgcat ggtgatctgt tcaatctttt gaatttggaa
     7021 ccgtccagaa attctatggt tcctgaagta agcttcttgg tcaaaattat aaagattccg
     7081 cttttttgct ggagagatga actcgccttc agcagtcaat tctattccct ttcctcggaa
     7141 gttaagccat gttttcaatt ctgcttctgt gtttacttga taagaaagct ggagcttttg
     7201 tttattctct gataaccttc cttccattgt caaaaaatta ccatttgcac gaatcgtatc
     7261 tgtaaataca gtaatttgcg cagatgttgt tttttcctct aaaaatgaat actcttgtag
     7321 cctataagaa atggataccg ttatgctaca caaaatacac aagtacgcga taaaacgtcc
     7381 ttcttttagc gaaaaccata aaaaaattcc ggcaagaagc aaacgataac cccaattgtc
     7441 agtgtaatac aacaaacaga taatcatcga cgtaattgcc ggaaaaatca accgatttcg
     7501 aacatgcctt tctaaaacgc tagacatgtt ttcctgttaa ttcattttga agttttgaaa
     7561 aatgctgtga gtctaaagca acttggtgta cagatacacc taattgttcg atcaacgctt
     7621 gggcataagg atcatttcga tagtcatgga gataattgat ccctttcaca ccagcttgca
     7681 acaaagcctt tgtacaggcc aaacatggaa aatgtgtgac atagatttct gaaccttccg
     7741 tcgggatacc aagttttgca cactgtagga gtgcattcat ctctgcatgg atcgttcgca
     7801 agcagtgtcc atccacgacg taacaccctt catctatgca gtggacatct ccactgactg
     7861 ctccattata gccgccagca atgatccttt tttctcgaac gagtgtcgct cctacttcta
     7921 aacgtgtaca cgtactacgt aaagataaca gtactgcctg agccataaag tactggtccc
     7981 aaggaatgcg ttcattatgt gacttatcag taactttttc tttattcgta tcgcatgctt
     8041 ttgaagattt ttctaacatg ttttatattc ctccgttttt tcaagttgat taaaaagtac
     8101 tcattgctta aacaaaatat tctttaaata taacgaatca tcctgtctga caaaagatcc
     8161 tacccttcac tgccgaaatg aagtccgtca tccattagcc cattataaaa attcttcgaa
     8221 tccatttgtt caagaaattc cgtataaaaa tcgatacttc acaatcatgg cgttttgcta
     8281 cttctgtgag aacagagata tattctttga tcaacttgtt attccgatat ttttgttttg
     8341 tctcatcgac tgctggtggc gtgataaaga aagcatgatc agaactatag tcttttttca
     8401 gctgaactat catccactgc agattttttg gtacgtttct ttagaaagtt gtttatgctc
     8461 tgctagatca ttcgtcccaa tgagaataaa tactttatct gctgtatagc taagtacatc
     8521 tttggcaaag cgttctcgta gagcttgact gttattgccg ctgacagccg tattcataat
     8581 atgaatccca gatattttat tttggagata gacattcagc attggttttt ttaatgtttc
     8641 tttgcgaaca atcaagctat ttcctatcca aaaatactac ctcctcgtcc tgccaagctc
     8701 ttagaattgg attattctta gtatccatta gcgtccattt attgctcgtg ccagtcgatt
     8761 ttgaatcatt ctatttaatt gtaacgaagt tttttagttt ttcaactgtc ttttcaccga
     8821 taccagaaat ctcctgcagg tcttccactt tctggaagga tccgttttct tctctaaatc
     8881 gaatgatttc ttgcgctttg actgatccga tcccaggaat ctgctgaagt tctgatggag
     8941 tggctgtatt gatatttatt ttgggaggct cggaatctgc tgtaggctgt tttccttccg
     9001 aggatgccaa ctgatcaaga tgttctggca gctcttcacc tacagcaggg atatatagga
     9061 cttgctgatc ttctatcttc gcagaaaagt tgattcgatc gctatccgct tcttctaata
     9121 atccacctgc acgctttagt acatcataga cccgttcttc tgaagaaaag gaatagatac
     9181 caggctcctt cacacttcct ttgatatcgg tgtacatgac ctttggacga gcatcctctt
     9241 ctacatcgtt tccttcagct gatggatctt ctccactgga actaaacgaa gaaagtattt
     9301 cttttgcagc agtctcttct gtttctctat aaaatagcaa atttgataat aaaaaaccac
     9361 ttgcgataca aaagataaga actatcatag acatccaaat gatcaatttg ggtgaagcta
     9421 gtattttctt gctgattttt tctatcattg ttcttttttc aaagaatgac cagtaaaaat
     9481 cattcattac tcctttcatt taaaattgaa gaaacagtga gctctggcaa aatcgtccct
     9541 tcaacatcta attacgtcaa ttttttcttt tgtttttatt tttatcgtga a
//
LOCUS       DAZUKR010000102         9547 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000102 DAZUKR010000000
VERSION     DAZUKR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.104"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..680
                     /locus_tag="ACG89C_002146"
     CDS             201..680
                     /locus_tag="ACG89C_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS4900669.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="ACG89C_002147"
     CDS             713..2941
                     /locus_tag="ACG89C_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4900670.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="ACG89C_002148"
     CDS             3070..4713
                     /locus_tag="ACG89C_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4900671.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="ACG89C_002149"
     CDS             complement(4786..5331)
                     /locus_tag="ACG89C_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS4900672.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="ACG89C_002150"
     CDS             complement(5354..5839)
                     /locus_tag="ACG89C_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4900673.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="ACG89C_002151"
     CDS             complement(5829..6641)
                     /locus_tag="ACG89C_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS4900674.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="ACG89C_002152"
     CDS             complement(6780..8312)
                     /locus_tag="ACG89C_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4900675.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="ACG89C_002153"
     CDS             complement(8421..9452)
                     /locus_tag="ACG89C_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4900676.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3185 a         1794 c         1845 g         2723 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttt
//
LOCUS       DAZUKR010000103         9359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000103 DAZUKR010000000
VERSION     DAZUKR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.105"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..758)
                     /locus_tag="ACG89C_002154"
     CDS             complement(90..758)
                     /locus_tag="ACG89C_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4900677.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            892..1179
                     /locus_tag="ACG89C_002155"
     CDS             892..1179
                     /locus_tag="ACG89C_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900678.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1595..3133
                     /locus_tag="ACG89C_002156"
     CDS             1595..3133
                     /locus_tag="ACG89C_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS4900679.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3130..4095
                     /locus_tag="ACG89C_002157"
     CDS             3130..4095
                     /locus_tag="ACG89C_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900680.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4082..5404
                     /locus_tag="ACG89C_002158"
     CDS             4082..5404
                     /locus_tag="ACG89C_002158"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4900681.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5467..5571
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5645..6388
                     /locus_tag="ACG89C_002159"
     CDS             5645..6388
                     /locus_tag="ACG89C_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4900682.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6403..7395
                     /locus_tag="ACG89C_002160"
     CDS             6403..7395
                     /locus_tag="ACG89C_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4900683.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7407..8144
                     /locus_tag="ACG89C_002161"
     CDS             7407..8144
                     /locus_tag="ACG89C_002161"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900684.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8134..8856
                     /locus_tag="ACG89C_002162"
     CDS             8134..8856
                     /locus_tag="ACG89C_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4900685.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8940..>9359)
                     /locus_tag="ACG89C_002163"
     CDS             complement(8940..>9359)
                     /locus_tag="ACG89C_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4900686.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2712 t
ORIGIN      
        1 tttttataaa gaagatttta ctgaataata tcattttagt ttacataatg atatttctgt
       61 aaattaaata aagtagtgaa aaataagaat cagtgctcta actgttcaag aggatcaagt
      121 ggttcaaatg tttctgtagt ctgttttgct tccaattggg gcaaaacaga tccaatttcc
      181 agatacttca ctttattcaa tgtataagat agaatagaac agctaagtaa tgtatacaga
      241 aaatgtgtga gatccaaata aagcaaggag acaactaaaa taccaaaagt aatcaagaaa
      301 agacttttgc caatcttaat atgaaaatat ttttcaatgg cttgataaat gatatctgtt
      361 ccgcctgtgc ttccgccgaa tcgaaagacg agaccgcaac caatgccaga aagaatacct
      421 gtcatcaaac tattgatcag tagactatct acagcaaaat gtatatgaag gctctcccat
      481 aaatgtaaaa agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt
      541 ccaaaaagaa acaatgagat aagcaataaa ggtaaattaa aaagcagtga agtgacagaa
      601 ggagcgaatc caaaaagatt atgcaaaagc aaagcaatac cagtaacccc accatctgcc
      661 agcttagatg gaatattgat tcctgtcaca gctaatccgt agattcctgc gcctaaaatg
      721 attccgcaca attctgtaaa taaatttaat tttttcattt ctagcctctt ttcttttgaa
      781 aattttcagc aacttcttta ttatactgac ttatactctt acttcaagag gaaaatgaaa
      841 aaaaattatt tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca
      901 gcagacttat tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt
      961 ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa
     1021 tttatctttt tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt
     1081 ttgatgaaga accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt
     1141 tatggagtga aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac
     1201 gtttatgatg tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt
     1261 ataatattcg ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg
     1321 gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag
     1381 tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg
     1441 atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt
     1501 tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg
     1561 tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag
     1621 tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc
     1681 tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa
     1741 aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt
     1801 attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt
     1861 tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc
     1921 gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt
     1981 agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat
     2041 catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt
     2101 gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt
     2161 gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc
     2221 catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc
     2281 cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct
     2341 gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc
     2401 gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta
     2461 tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag
     2521 tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt
     2581 cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag
     2641 atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca
     2701 gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca
     2761 agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca
     2821 agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa
     2881 ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc
     2941 accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa
     3001 attaagcgga aaaataacgg aggatcaaat gattgatatg aattaccaag tcaatgtcga
     3061 aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga
     3121 ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag
     3181 tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt
     3241 ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca
     3301 gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg
     3361 tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa
     3421 aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta
     3481 gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga
     3541 gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca
     3601 gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta
     3661 caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac
     3721 aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt
     3781 atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat
     3841 ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga
     3901 tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg
     3961 aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga
     4021 agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga
     4081 aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta
     4141 tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac
     4201 ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa
     4261 tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga
     4321 acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt
     4381 agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat
     4441 tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc
     4501 tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt
     4561 gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc
     4621 tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt
     4681 aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat
     4741 tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt
     4801 agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc
     4861 gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc
     4921 agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc
     4981 aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg
     5041 gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc
     5101 cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt
     5161 ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa
     5221 ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca
     5281 agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga
     5341 gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa
     5401 ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa
     5461 aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac
     5521 ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg
     5581 ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa
     5641 caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca
     5701 gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt
     5761 ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga
     5821 agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga
     5881 ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc
     5941 aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt
     6001 gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag
     6061 gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat
     6121 ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc
     6181 tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt
     6241 agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat
     6301 catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg
     6361 gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt
     6421 ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa
     6481 caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat
     6541 catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc
     6601 caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca
     6661 caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata
     6721 ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca
     6781 gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt
     6841 ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa
     6901 ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc
     6961 caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac
     7021 tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg
     7081 aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa
     7141 aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat
     7201 gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat
     7261 acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct
     7321 tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat
     7381 cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca
     7441 ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat
     7501 cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc
     7561 agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag
     7621 catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg
     7681 ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc
     7741 tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga
     7801 tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg
     7861 atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga
     7921 agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa
     7981 tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga
     8041 ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc
     8101 aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta
     8161 gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat
     8221 tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc
     8281 ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct
     8341 tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta
     8401 ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag
     8461 aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct
     8521 taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca agactatatt
     8581 gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa
     8641 ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca
     8701 gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag
     8761 ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat
     8821 caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg
     8881 tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct
     8941 tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa
     9001 gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa
     9061 gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt
     9121 gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt agcagcagtt
     9181 tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga
     9241 accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac ggctgcttga
     9301 ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagta
//
LOCUS       DAZUKR010000104         9334 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000104 DAZUKR010000000
VERSION     DAZUKR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.106"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..370)
                     /locus_tag="ACG89C_002164"
     CDS             complement(<1..370)
                     /locus_tag="ACG89C_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4900687.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIE"
     gene            complement(360..2534)
                     /locus_tag="ACG89C_002165"
     CDS             complement(360..2534)
                     /locus_tag="ACG89C_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFS4900688.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2550..4892)
                     /locus_tag="ACG89C_002166"
     CDS             complement(2550..4892)
                     /locus_tag="ACG89C_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4900689.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4914..7457)
                     /locus_tag="ACG89C_002167"
     CDS             complement(4914..7457)
                     /locus_tag="ACG89C_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4900690.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7467..7865)
                     /locus_tag="ACG89C_002168"
     CDS             complement(7467..7865)
                     /locus_tag="ACG89C_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS4900691.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7946..8230)
                     /locus_tag="ACG89C_002169"
     CDS             complement(7946..8230)
                     /locus_tag="ACG89C_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900692.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8254..9252)
                     /locus_tag="ACG89C_002170"
     CDS             complement(8254..9252)
                     /locus_tag="ACG89C_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4900693.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2653 a         2001 c         1461 g         3219 t
ORIGIN      
        1 tctcaatttt tttgacgttt ctatcatgaa tcacgagaaa acttgggaca cttttaacat
       61 ggtaacgttg taaaaaagtt tgaagttctt cttgtcttgg ttcttttctt gtttcaattc
      121 gatatacatt cctagagaat tttttgtcgt atctttccaa gaaagattca acttcttggc
      181 attttttaca atcatttctt gctagataca caaaaaatga ttgattcgtt ttttgaagtt
      241 ctagtacttc ttctaaatta ctattgatta gttttccatt tgattcttcg ttagttttta
      301 tcagtttaaa acaaccaaat aagaggaaac taacaataag tatcctaaca ataactttct
      361 tatttcgcat attgtttctc caaatttggt ttccgaatat agacgactcg cggtggttcc
      421 tggacgtctt cagctgttcg atacaccaca aattcgcctg ttctcaatgc tcgaatgcga
      481 ttcggattga atttaaagcg gtctacgtct cgtttcgtgc ctctttctcc tgtccaatca
      541 acctttccca tatcagaata acctgcttct tgtgtcgtca tttccgttaa ttcaatgtct
      601 tcttctgtcc ccattaaatc tgcccaataa tcaatttctg tcgggtcatt tgtcttccca
      661 ataaagaacg tattcacatt cccaatcaac tgttttcgaa ggacaggatc aattctatca
      721 atgtccgcaa gcgtttgagg ggcatacgcc gcatgcaccc cagcgcctcg tcccttattg
      781 actaagtcta taaggttatc atttagataa gaagctggtt cgtcaaatat tactaggact
      841 ttcgagcgat tggctgtttc tcctgcttgt ttatacactt ctgacgcaaa gaaattaata
      901 tctcccacaa gcatttgagc caatgtccga ataaactcac tataaatcaa tccgtttaac
      961 gagatataaa caatctttcc ttctttaaca acttcttgaa tatctaattc tctttttcct
     1021 ccagaagtat caaagagttc accaagctca ctatccaata acatattgat attggtctgt
     1081 aaattctcag aagtcgcata caaataaaac gggttcttct tatgttcgta acgaacaaac
     1141 aaccgtttaa acaacgtttg ctcgatttct gtcatgagag aaatgttttc ttttaataat
     1201 agataatacg tttctaagtc aacggtttta cgatcatgtt caacgattcg ctcttcactt
     1261 aactgttcca catcggcaaa atcacgttct gttttgtcca tcgcttcatt ctttttcgtt
     1321 tttttcttcg tggttttctt cttttctacg accacttttt ctttaaataa atctggattt
     1381 ttcaactgaa tacgatccgc aaacaaatgt aagacgtttc gtggcaataa ataatgttgt
     1441 aaccaaacta gatttctttc aattttatgg ctcttttgga actcgtctaa aagctgaatc
     1501 gttacctgta ataacgcttt ggctgctgtc gaataataca cgctttctgt ttctgctaga
     1561 ttggttaatt tatctcgaat ggcaatatca ttgccatttt ttacaggatt ataacgcaaa
     1621 tcgccaccgt ccacaaagac ttcaatcggg cgattgtatg cctctgccac ttcttttaac
     1681 gcttgtaaag tctctcctgc tcctttccca tcgactaaga taacggggat acgaaactta
     1741 cacgcatgtt ctgctagtaa ttggatcacc gtcgtttttc ctgaacctgt agtcgctgga
     1801 ataatcacat gttggttcaa ttcactaaag ttcatcacat aagggagccc atactcatct
     1861 gttcctaata gaatcacttt ggctaatgaa gccagttgtt tattccgcaa agcaacgtct
     1921 tttatgacaa ttgctacctg gtacgcttgc tgatactctg ctgtatattt ttgggactcc
     1981 tgaaatttat tcttccgacg ttccttgtat tctttactat ttagcaaagc tgtttgccgt
     2041 tctttctttg aaacaacttt tcttttttca ataaaagacg catacgcaat caaacaactt
     2101 cctgcacctg tcccacctaa aaggagggca aaaatcgttt gtccattaaa ctgcaacgca
     2161 tgagaaaaca agtttaatgg aaataataag taacacgaca aataccctct aggaagaaca
     2221 aaaataaaac aaaatagatt taatagcgta aaacctaacg caaaaataaa aaaaagagag
     2281 cttcttcgtt ttaagaagct ctcatactca aagtcataaa tatgtgcacg tgtctctttt
     2341 ttatctatca taaagacaat cgctaaagga atactgccta caataaaaaa tggatataga
     2401 caaacaccta ataaagtgac gataaaataa agttgttgaa accgactttt tggcgtttct
     2461 ttttcttgtt ttcttttgcg atcgacagaa aacatttgct caaaatgtgt cggtcgtttt
     2521 ggtgcattca tcatactatc gatttcctcc tatcgttttc gttgtttcat ttctcttcga
     2581 taaatcggtt gtttttctcg ttttcgtttc atttttgctt gagttgctcg aaactcactc
     2641 cgtgtcttcg gtgcttgttt tggtgatttt gtttgtttca tggttgagta tgctttagat
     2701 acggttgaag gcgctttggt ttgtgctgtt actcgattga ctgctgacat atgttcccga
     2761 accccctctg gacgtcgaac agatttcggt gtggtagaac ctgttgctcg tggtacattc
     2821 ccttttctta gtggtttttg taccgcatgt ttttgtcctg caactgtccg ggttttcatc
     2881 gaacttgttt ttgttacggg agtaagtgcc tccttccgac gacgacttgt ttgtccctga
     2941 cgacgttttc gatcgttcaa ttctgttcta ggcgtatgct tctggccata agacttgtgt
     3001 ttttgctttg gattcgctaa tgacaccacc attgcagaac cctgattgcg atacggacta
     3061 cgagacgcac cttttctact tctttctctt agactaggat tgccttgcaa ttctcgttgt
     3121 aataaatcgg ctgtttttct tgcctgtttc tccattgctg ttcgttgcat acgttcttga
     3181 ctttgctctt ctgcttgttt cgcttgatca tgcaagtctc ttgctttttg tgccatacgt
     3241 tccatacgat ttggtgcttt atgacgaggt tgttgtgcgg attcacgccg aaaagcactt
     3301 cgtgtggctt gtttcgccgt ttgtggcata cgaacgtctg agccaccccc acgttttcgt
     3361 tgacgttctt gtctcttccg tgcgttttct ttttcttttt gtttctgtgc ttttctcgct
     3421 tctttgcgtt ctttcaaccg ttgtttggct tcccttctca attttgcttc tgccccaaat
     3481 ggatttcccg caaaagctaa cgctcctcga acacccattc ttccagtaaa tagactgcct
     3541 aaaaagcctc ggaacaaata aattcccaac ggcgcaaaca aataaacaat gatcaagaga
     3601 attttttccc atggatcgtc ttgtgtacta cttaattgga acccgtaact aagaaaagaa
     3661 gaaaaaagga tacatgcaaa acctgtcatt ccttttaaaa acgtcaaaat accaaaaact
     3721 ttggcatagt taccaaacac attcattgtg acaagaaaca atcctaaaaa aagtaagaac
     3781 ggcaacatcg gtactaacaa tagttgcata gttccaataa tcaatcgaaa catacttaag
     3841 acaaaataaa caacggtttg aatcagattc acgacaaaat aaaacaaggc aaatgctgta
     3901 tttttccaac tattgtaata ttgtatgttc ttgtttttca gtgtatctgc ttcatactct
     3961 gtgactttgt catgaatttt ctcccctatt tttgtaccta aatcattgtc caataaaaga
     4021 ttgattcgat catattcttt tccagcatat ttgactttct tctcacgaat tgtgtctaca
     4081 ttggttgtgc cataatttaa caacagatat ggttcataaa cattactata aaagacccga
     4141 gccgccatca atttgcctgc cgtttccact tttttatccg ctgttacctc ttctgtttct
     4201 tttgtttcat aatttgtttc ttcttcaggt gttaacactg gattgacctt cacgaacgcc
     4261 gtttccactg tagaatctaa agaaaaaaga acatcaaaaa gattcttatt tgtcccagaa
     4321 tcttttaata cgcttaaccc agtaaaaata aggatcgtca taagaaagac acggagtgct
     4381 tgtttcattc gagcctctcc agcatatttg attgccatca taattgccga aaaactgatt
     4441 ccaatagttc ctgcaatact taacatattg tccgcaattt tttgtgttaa gttttgaatg
     4501 ggttctcgaa tggcggaaat aatatctaat tcaaagagaa attcaaccat tgaagcattg
     4561 aattctccta acatttttac gccactccaa acaaaatttt tcatcactcc tgcaacattg
     4621 tacgatagct tttttttaag gttcattgac tcatagtcat tggtcatcaa ttcaaacgaa
     4681 ttctttttgt aatttttata caattgatcc acatcagtcg ttacttcttt tacttctact
     4741 ttatcgttgg cattctctcc ttcagaacga tcggtttcag tgacacccgc accatcattc
     4801 atttgatcgg cagaaacagt agccccttgt agacagaaaa aaagaccccc taataaaagg
     4861 agcgtccacc atttcagaga tttttttcgc aaaaaaacca cctctttttt gttttatcgt
     4921 tgtttttcat tttctaacat tgcttgaatt ttttcatctt gattttcagt agaagaaaca
     4981 gccaataacc aactatcaaa caacacatca atggcaatcg gttgatttct tcccaaatga
     5041 tcctggaata aacaggttcc tgtttgtaat tgttccatca tttctaaatt ggctgaattc
     5101 acttccatat caaagaagga caatagtttc tctcgtgctt ctttttgttt cggacgaaac
     5161 gcaaatttgt aagaaattaa ctctttttta tcttcttgat cgtaatcttt aaacgcttgc
     5221 acaacaaggt aaatatccgt attattcgca cgtccttttc gcaagctatc atcaatcaaa
     5281 tagcttcctt gtgcggtatc ttcataccct tttgcctcgt cataaataat cgcagcgtct
     5341 tcttctttgt ttgtagaaaa gatatacgta aacttcataa tgacttccat aattgccatt
     5401 cctgcaagtt gttcgctatt taatcgaccc gaatcaatct ctttttgtgt cggaatgcgt
     5461 attccttgtg tccctaacac gttgatttgt gaatcaaaac gtatcggcgt actgtgctca
     5521 ttcccaatga gaatctttcc taaaccaacg ctatgcccta aaatcaattt agctagttct
     5581 ttgtcacttt ttcgaatttc ttcgataact tgacttaacg aatgcttacc tggcatggtg
     5641 taaactacat cgatggcgtc ttggatcacc gttttcttgt ggctcgctgt ttctcgattg
     5701 gtatctactt ctcctaacat tcctaacaca tttttagcgg tttgaattgc ctcttctttt
     5761 ggtaaaaaca atagaggatc aagcatccct cgacagcttt catctgaaga taacgaaata
     5821 aaattaattc gttcatacat agccacaaac tctggtatgt gtccgaattt ttcaagtgcc
     5881 tttaaaaaga acttttgggt ttcatttttc ggatcaatat aaagaatttt ttgacctaaa
     5941 aacatcagcc ataaaaaaat atattttaca aggaccgatt ttcctttacc tggtggacca
     6001 ataatcaacg tatttccatt ggtatgttcc gcgccttcaa tacttttctt tgttaaattg
     6061 ggataatacc atactagttt actagatagc gctaacgctt ctttcgcaga tttaaacttt
     6121 tttgcaataa caatccgtcc taaaggaaat cctttcttat ttcctacttc tttctttaag
     6181 tccaagccat aatctgccac aaaacctgtc gtagcgacct gctcgtaact acgaaaacgt
     6241 tgcgtacttc caattaagca ttgattaaac aacgttagct gatccgctac cgatcgataa
     6301 aatttgaaat ctgtagactc aatcgcaaat tcaatatctt tgactcgttc ttccaattct
     6361 tcttttgaag aagcagatac aaccagcgtg acggtagaat aacacaatcg tttttctttg
     6421 cgcttcagct tatcttttac atccattaac cgttctgtac caaaatttaa tacatcgtct
     6481 gaatctaaaa tgccgtcaac ggtctcatat tctttttctt gttcataaat ccgtccagcc
     6541 attttattaa tttttcgttc atcagatttt gaatgctcaa atcgaagtcg aatatgcgta
     6601 tcaattggaa aagataagct atcctgaaga ttttgaataa agctactccc atacatggct
     6661 aaaggagcgt ccgtttgact taaaaacgtc gaataatgcg tttgattcaa ctgttcaatc
     6721 gcaatgtatc ctgcttcatt cgaaatgacg ccctcgcaaa tttccattgg cgtaagctgt
     6781 ctttcaggca atttttcttc tgatcgatgg aaaaaataat aataaatttg tccaatctct
     6841 tgggcagata agcgctctat cggcttataa ttggacaatt ccgtatagag ctccttttct
     6901 aatgtttcat acgttggtaa cacactactt ataggaactc gttgtcccgt aatacgatag
     6961 ataaactttt taaaaacatg cttaagcaat tgaaaatatt cgatcggatc agacactttc
     7021 atttctgtag tgaaacgcac aaacagatac gttcgatact cgtttaattg cacttcgtct
     7081 tttaaaatct ttcctgcctg ttcaaagtac actttcccaa gatctgcgca ttctcccttt
     7141 accaaatctc gaatggtttc ttcaatgtgc gtatctaggt taaattgacg tggaatattg
     7201 ataaaatgat agtcatattc atcatgtcca agtatgcctt cgccatcttg aagatagttc
     7261 ttgaaaaaat caatgtcatt taaaggaaat acttgggaag aaagttgata gcctacccac
     7321 acatcatcat ttttggtcaa aacaaaattt cggataatgg cgtctatcgg aaattctaat
     7381 cgaggaagct tcgttttttt ctttttgaac tgccaaatcc cgataataaa gacgaccaaa
     7441 agaaacaaaa acgccattta ctcgactcac tccttccatt tgtgtaggga atacgtcact
     7501 ttttcatgga aaaacggcac atacatttca tgttcaaagc gttgtttccg tttctgataa
     7561 taggacgttc gcccaattaa atactcgaaa aagtgtttat tgtcccattt cattgaaaac
     7621 aatgtccaaa cgagaacacc aatcccaaaa atcataatca accaggactt tcgaaacagt
     7681 gtctgcaaga aactaatttg attcacatac gcaatcaaaa ataggatcaa aagaatgaag
     7741 agaaataccc cacctatgac taattgttcc atgcgaatgc cattagaaat ccagggaatt
     7801 ttttgtcctt taatcccgta aattttgtga gggtgcttaa actcctttgt atagttgtat
     7861 tccatgttca ctctacctcc ttttacaaaa aaagaagcga ccagaagttc taatcttctg
     7921 atcgcttttg cagtttgtct ttttcttatg ataattgtat ccagggcaca atcatttgcg
     7981 ttacccccac gttctcttca ctatccttaa taaagataat gttctttagc cattcaccaa
     8041 tatcaacggc attcgataac aataagacga gcattatcaa aataaagacg ccaataatgg
     8101 caaacgctgc ccgcgcaaag ccttgggtcg cataagcacc aaccattaga acaatcgcaa
     8161 cgattgccaa tagccaaatg acttgtccag atacatcatc aaataacgat ttaagatcaa
     8221 aaagtttcat ttccattctc ctctaagttc attttattca tttaacgtag taacaaaata
     8281 actatcgcca cttgttttta cttccattga aaagtttgat tggaactcgt ttcctttctc
     8341 aaaatagaaa cgatacttcc cttgcaaaaa gtagtttcct tctttatttt ttactgcatg
     8401 atccagttgt acattgatca attcgccgcc ttctaaccct ttttcgttag aaatcaatgc
     8461 caatttttca tcatttttga catataactc aaaaaaccgt ttcacaaatg cttccagctt
     8521 gatttcttcg ctttcagtga ccgcttgccc agaagaataa aatcgatttt cctgatagtt
     8581 cacagaattg gtattcttac tctcagaaag attcaaaaat tgaacgccat caagtacttt
     8641 taaaacattg ttttgataac tgactggaaa agaaagttga ctgcttttcc aatgttctcc
     8701 ttctgtaaaa gtaacctcat aaaataggcg atacattgct tgtgtcaaat cgatttcttc
     8761 tagtttaata aagcgaacat ctttcgctac tcgattggta gccgtcgtta caaagcctag
     8821 atcagacgct tccaatcctt gtgctaaata atgattgacg gaagcctccc attcgtttcg
     8881 tttttctggc gaataagtaa ataacctttc aataaaatgt tgtccctcgt acttcaattg
     8941 ttccgtagaa gacaaggttt ggttgacttc agttaacaat gtttctggat tgacttgttt
     9001 tttcactgct aaacgcgtta atgtatccac tcgtccaaag tttaggagga gcacgacacc
     9061 cattaacaga aacaaacaaa aaaaagcgcc tgaaaataca ctcatggcac ttttttgcga
     9121 aaattggatc attcgatctg acggttctgt tttctttttt tggaacaaaa aacgtttctt
     9181 ttgtttcttt tccttctgtt gtttcggtgg cttagtttgg aacgcttgtg gattaaattt
     9241 tgcattactc atgagaaatg ccataccgtt ctaacaataa tttcggtatt ttgtccacaa
     9301 tcactacttc gtggacaacc acacttgccc cctc
//
LOCUS       DAZUKR010000105         9192 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000105 DAZUKR010000000
VERSION     DAZUKR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.107"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="ACG89C_002171"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="ACG89C_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4900694.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1552..2973)
                     /gene="tyrP"
                     /locus_tag="ACG89C_002172"
     CDS             complement(1552..2973)
                     /gene="tyrP"
                     /locus_tag="ACG89C_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS4900695.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="ACG89C_002173"
     CDS             complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="ACG89C_002173"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4900696.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5384..6640)
                     /gene="tyrS"
                     /locus_tag="ACG89C_002174"
     CDS             complement(5384..6640)
                     /gene="tyrS"
                     /locus_tag="ACG89C_002174"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4900697.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6752..6957)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7220..8521
                     /locus_tag="ACG89C_002175"
     CDS             7220..8521
                     /locus_tag="ACG89C_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4900698.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8590..9189)
                     /locus_tag="ACG89C_002176"
     CDS             complement(8590..9189)
                     /locus_tag="ACG89C_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4900699.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2771 a         1910 c         1635 g         2876 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag acgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt tttatttttc
     1561 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
     1621 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
     1681 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
     1741 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
     1801 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
     1861 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
     1921 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
     1981 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
     2041 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
     2101 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
     2161 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
     2221 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
     2281 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
     2341 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
     2401 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
     2461 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
     2521 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
     2581 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
     2641 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
     2701 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
     2761 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
     2821 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
     2881 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
     2941 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
     3001 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
     3061 gaggggattc tttgagtcta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
     3121 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
     3181 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
     3241 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
     3301 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
     3361 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
     3421 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
     3481 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
     3541 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
     3601 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
     3661 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
     3721 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
     3781 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
     3841 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
     3901 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
     3961 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
     4021 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
     4081 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
     4141 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
     4201 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
     4261 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
     4321 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
     4381 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
     4441 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
     4501 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
     4561 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
     4621 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
     4681 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
     4741 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
     4801 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
     4861 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
     4921 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
     4981 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
     5041 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
     5101 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
     5161 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
     5221 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
     5281 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
     5341 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
     5401 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
     5461 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
     5521 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
     5581 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
     5641 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
     5701 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
     5761 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
     5821 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
     5881 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
     5941 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
     6001 acgcttctac ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
     6061 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
     6121 agtcgataga ttgcagaatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
     6181 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
     6241 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
     6301 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
     6361 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
     6421 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
     6481 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
     6541 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
     6601 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
     6661 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
     6721 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
     6781 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
     6841 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
     6901 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
     6961 attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt
     7021 ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca
     7081 attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact
     7141 ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa
     7201 aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc
     7261 tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa
     7321 agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa
     7381 gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat
     7441 tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac
     7501 actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt
     7561 ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg
     7621 atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca
     7681 aaatgatttt gttttagtta atttaaatga tttgatccaa aaagacgaac aaggaaatct
     7741 ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga
     7801 tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa
     7861 taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga
     7921 catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc
     7981 aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc
     8041 tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga
     8101 acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac
     8161 tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc
     8221 tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca
     8281 gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt
     8341 ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa
     8401 tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat
     8461 tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg
     8521 attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
     8581 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
     8641 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
     8701 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
     8761 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
     8821 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
     8881 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
     8941 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
     9001 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
     9061 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
     9121 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
     9181 accgttcatt tg
//
LOCUS       DAZUKR010000106         9157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000106 DAZUKR010000000
VERSION     DAZUKR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.108"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..665
                     /locus_tag="ACG89C_002177"
     CDS             18..665
                     /locus_tag="ACG89C_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4900700.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            749..1693
                     /locus_tag="ACG89C_002178"
     CDS             749..1693
                     /locus_tag="ACG89C_002178"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4900701.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1828..3279
                     /locus_tag="ACG89C_002179"
     CDS             1828..3279
                     /locus_tag="ACG89C_002179"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS4900702.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3300..4136
                     /locus_tag="ACG89C_002180"
     CDS             3300..4136
                     /locus_tag="ACG89C_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4900703.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4136..4795
                     /locus_tag="ACG89C_002181"
     CDS             4136..4795
                     /locus_tag="ACG89C_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4900704.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4921..5610
                     /locus_tag="ACG89C_002182"
     CDS             4921..5610
                     /locus_tag="ACG89C_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900705.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5616..6572
                     /locus_tag="ACG89C_002183"
     CDS             5616..6572
                     /locus_tag="ACG89C_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS4900706.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6616..7128
                     /locus_tag="ACG89C_002184"
     CDS             6616..7128
                     /locus_tag="ACG89C_002184"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900707.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7384..7932)
                     /locus_tag="ACG89C_002185"
     CDS             complement(7384..7932)
                     /locus_tag="ACG89C_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4900708.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8021..>9157)
                     /locus_tag="ACG89C_002186"
     CDS             complement(8021..>9157)
                     /locus_tag="ACG89C_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFS4900709.1"
                     /translation="LAFLISSNKKAIKLKPLIVMFVLQLVLGFVLLRTTFGTAVVSVL
                     AKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISAIIGILRYIKILPLFM
                     KAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFLSEKRLFTMSVSAMST
                     VSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEEKEDELIIEENKEQTF
                     FQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGITFQEILGYIFAPLAFI
                     SGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTAIISVFLVSFANFSSI
                     GIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLLV"
BASE COUNT         3025 a         1680 c         1864 g         2588 t
ORIGIN      
        1 caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt gcagctttgt
       61 ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg tcttccagta
      121 gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca tcttcgtcta
      181 gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc
      241 gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt ttggaattag
      301 atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg
      361 aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa actctagatc
      421 gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat aaattgatca
      481 cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg gcaacagatt
      541 gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa gacggcaatc
      601 aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact gaacaagatg
      661 attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat acactagaca
      721 aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg ccaatcatat
      781 ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc aggaattaga
      841 tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga tcaatgaacg
      901 aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca attctatggg
      961 acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg aaaaagtgca
     1021 ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc aggaaaactt
     1081 ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat taaatttatc
     1141 ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca cttatgaaac
     1201 actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc ctccttattt
     1261 tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat tgacctatgt
     1321 caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag aagcagtagt
     1381 catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc cgactgcttt
     1441 ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca ttggtacagg
     1501 tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa gcatgttaca
     1561 gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca taaaagaatt
     1621 aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag gcaaattacg
     1681 caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt ttataaaaaa
     1741 cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa
     1801 aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc aaaagagctt
     1861 aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa aataaagaag
     1921 caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag
     1981 ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc tttaaaaatg
     2041 ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt tgaaacacac
     2101 cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct agttcctttt
     2161 ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt catctatggc
     2221 ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg aggagctgct
     2281 gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg tggttctatt
     2341 agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg gacgatgcca
     2401 gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg cttgacagtt
     2461 tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc cggtgcgcca
     2521 tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt gaagatagct
     2581 ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact tgctgtaaaa
     2641 aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc ttacccacta
     2701 gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac agcagcaatg
     2761 atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact tgaacccatt
     2821 tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat cgattctctg
     2881 catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata cgatctattc
     2941 ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca aagtgatgcg
     3001 ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt agacgttgtt
     3061 tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc taatctgact
     3121 ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc tctaggaatc
     3181 caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta tctatttgaa
     3241 gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg agaagaagaa
     3301 tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta agcgcttgcg
     3361 gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa aagaaagaat
     3421 tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat caaaatgaat
     3481 tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat gtcaaaatct
     3541 cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt
     3601 ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc ctttcagaga
     3661 ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca ggaatccact
     3721 cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc tatatgcgtt
     3781 tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat gatcagtatt
     3841 taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat ctgcagaaaa
     3901 tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt tatttcaatg
     3961 aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg
     4021 gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa aaatattttc
     4081 aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta
     4141 ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac tgcaaggctt
     4201 ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg tagggttgct
     4261 tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc gtttttattt
     4321 atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt tcgggttacc
     4381 ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca gcagtgcatt
     4441 tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt gggacgctgg
     4501 tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc cgcaagcttt
     4561 tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag gtacttcgtt
     4621 agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag gcggtcggac
     4681 attcgattat ttttctctct atattttagt tgcactgatt tattgggtca tttgtatcgg
     4741 gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa tctgaaaaat
     4801 agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta tattctgcct
     4861 agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac
     4921 atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg catcgagcaa
     4981 ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt agatacgctt
     5041 agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct gaaagtgaca
     5101 aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa ttattttgtt
     5161 ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat aggtgtttta
     5221 gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt tctgattgcg
     5281 aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg
     5341 gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt
     5401 ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga tccattttct
     5461 aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat caatgagttg
     5521 caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca acagcagatc
     5581 cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat ttaagaggaa
     5641 aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc tccaataaaa
     5701 tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg atcgaattat
     5761 ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga caattcaaat
     5821 tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc gtccctgcac
     5881 agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg tttcttgttt
     5941 tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca ggagttgatt
     6001 cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa aacaaaattg
     6061 tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat aaaacagatg
     6121 acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga ttttctgatt
     6181 tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg cttgctatcc
     6241 atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc tctgacagta
     6301 acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa atgaaaaaat
     6361 ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa ttattggctc
     6421 tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca cttatgcatc
     6481 atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa tattattcaa
     6541 aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa aaaggaatag
     6601 aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga agaagtggta
     6661 gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca atggcaaaac
     6721 gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga aagctatgta
     6781 ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt ggatcccgtt
     6841 tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc gattcatcgt
     6901 gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt agaacatttg
     6961 ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta tccagctaat
     7021 gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat gattgaattt
     7081 ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact ataaaatcat
     7141 ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat agccaagagg
     7201 ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa
     7261 tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc ccttcatcga
     7321 gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta ttattcgata
     7381 catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg ctccatcgtt
     7441 tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta aatcttctac
     7501 ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt tcaggcgcga
     7561 ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc cgaaatactc
     7621 atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt
     7681 ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt cccactctgc
     7741 aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat aataatcctc
     7801 ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg
     7861 ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga taaatacata
     7921 gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat
     7981 atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc aagccagtca
     8041 caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat ccgtgttttg
     8101 cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg atcccgatag
     8161 aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta cgctcagtaa
     8221 aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta accagtttag
     8281 tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg atgaaggcaa
     8341 gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc ccgtggaaga
     8401 tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca accgtgatcg
     8461 ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc tgttctttgt
     8521 tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt acaattgaag
     8581 ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat ttagaatcga
     8641 tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc gatacactca
     8701 tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa ataaaaactt
     8761 ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg cccatcccgt
     8821 tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc ttgatatagc
     8881 gtagaatacc gataatagca gaaataaata caattggcat caatacggaa agaaagaaag
     8941 gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag
     9001 caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca aaagttgtac
     9061 gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt ggtttcaatt
     9121 tgattgcttt tttgttactg ctgatcaaaa aagcaag
//
LOCUS       DAZUKR010000107         9152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000107 DAZUKR010000000
VERSION     DAZUKR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.109"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..984)
                     /gene="ispE"
                     /locus_tag="ACG89C_002187"
     CDS             complement(133..984)
                     /gene="ispE"
                     /locus_tag="ACG89C_002187"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4900710.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(1138..3711)
                     /gene="mprF"
                     /locus_tag="ACG89C_002188"
     CDS             complement(1138..3711)
                     /gene="mprF"
                     /locus_tag="ACG89C_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4900711.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3852..4658
                     /gene="proC"
                     /locus_tag="ACG89C_002189"
     CDS             3852..4658
                     /gene="proC"
                     /locus_tag="ACG89C_002189"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4900712.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(4737..6293)
                     /gene="rny"
                     /locus_tag="ACG89C_002190"
     CDS             complement(4737..6293)
                     /gene="rny"
                     /locus_tag="ACG89C_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS4900713.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6704..7753)
                     /gene="recA"
                     /locus_tag="ACG89C_002191"
     CDS             complement(6704..7753)
                     /gene="recA"
                     /locus_tag="ACG89C_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4900714.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(7862..9103)
                     /locus_tag="ACG89C_002192"
     CDS             complement(7862..9103)
                     /locus_tag="ACG89C_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS4900715.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT         2613 a         2033 c         1589 g         2917 t
ORIGIN      
        1 atgtatattc ttgattaaat cgtaaaatgg atgagttgct gctcatatct agccatttca
       61 ctacaataaa aaactgaaga ataccatttt aggattcctc agtttttcat cacctatttt
      121 acttcttata ttttattgca gggttcgtac taaataaact tcctcacaaa aaccttttaa
      181 cccattatat actctctgcg ctcttgaata atgttgacac aaggcaaaaa cggttggtcc
      241 gcttccactc atcaatgcag catctgctcc gtacttgatc atacgctctt tgatttgatg
      301 gatgactgga tgccgctttc ccgttatatc ttctaaactg tttcccattt ttttgatcat
      361 ctgctgataa tcttgctctc ggatggcttc agcaagtccg tggatgtctt gatgttggat
      421 ggcttgcgta tctacttctc taaacactgt acgtgtagag acactcattc ttggtttgac
      481 caacacaacc cagcactgtg gcatagaagg taatacttgg atttcttccc ctttgccacc
      541 gacaaatgcc gtacttccgt agacacagta aggaacatct gtaccgactt ccaaaccgat
      601 ttctgccagt tcttctaacg aacagcccag attccataat tggttgatcc ctcttagagc
      661 agctgcacag tctgtacttc ctccgcctaa tccagctgct actggaatat tcttcttaac
      721 aaagatcttg acaccggttt tgatattgta tttatttttt agcaataaag cagcttgata
      781 aacattgttc cgttcatcta ccggtaaaaa tgctttattt gtttctacaa tgattttgtc
      841 ttctgttatt tcttcaaaaa atagatgatc tgccaagtct acgctcgaca tcaccatttc
      901 caactcatga tacccatctt gccgtctata taatacatcc agtcctaaat ttatcttggc
      961 tggcgctttt tcaatcaatt ccatcctctc acccattcga aaagtctaag tcgcaagtat
     1021 tctatcatag ggaaaaataa aagtatagct tatgtctgcc tagtggttga ttttgtcatc
     1081 gtttcaacag ctttcacaga attttctctt cataatccaa acaagttcta gtgattctta
     1141 taattctgtg agcacttctt ctgccaaact cggaacattt cctttttctt ttcctatcag
     1201 actgagtagc tgcatggtag caaatccgaa attacttcct ttcctataag caaaataacg
     1261 tccttcccaa tgatcgacat attttaattt cttttcataa gcagactgga aggaatagaa
     1321 aaaattacca taattatata cgatatgcat gactttttct tgaaagaaag aaaaatctgt
     1381 ttctccaaca ttagctaatg gagctaagcc tagattggct tccgagtagc ccattttctc
     1441 gtatacaata aacagattgg ctaacagata gtcagctaat tcctcagatt ctttaggtaa
     1501 gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg tgatgaagcc
     1561 aacgactttt tgatctttac ggacaatacc gacaccgcta gataatacat atgatttatc
     1621 aaatcgtccc acagagaagt atttttctcg tgcaccttga agccattttt gtgaaacttc
     1681 atttacagct tgatgtaatt cttccggcag ttgatcgtac caagtaaaat ggtatccttc
     1741 tttcatcaaa cgttgatatt caagttgctg actcagccga gacaagtctt ctgttgctag
     1801 atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa cccaattcat gaagcatcac
     1861 tacgtagctt tctgatatct tataaaacgc aagctgatag cctaaacaat ctgcttgttt
     1921 cataaactga ttcgttgctg cccgcatcat tttaggattg ccaactggat ctcctaaaac
     1981 aaagcatttg tttcctttga tctgataacc aaaaacgact tgatcttggt cgtcttcttg
     2041 gtaataaaaa taactgtaac ctgacaagcg aagctgatgg cttgtagctg tgcctccgta
     2101 tgtttccaat aaacggtcaa aacggtcttt ttcgtactca ctgccaagct tcgtagtatc
     2161 ttcagctaaa tattgataaa gcgccactaa agctagcaat gagatgccca aaccaataag
     2221 ccctgagaac cacacatcat ctgaaggaaa caaaataaaa tgatttcctg ttattcgacc
     2281 gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa acgataaaca aaaagccgaa
     2341 caagctagca tcaaagatta tagctcccca agaataaacg aattttgttc gataaaattc
     2401 ttttctggat aaccacacag ctaacagaat caagacatat accaatatca actgccaaga
     2461 taacgtacgt gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg taggcagaaa
     2521 tgcctttttg acttttactg aaagtgctct agcaaggccg agtaataaaa agccaaccaa
     2581 tagattcact gtctgatcta aaaaattgaa tgagaacggc aataatactt gaaatagccg
     2641 actgattgca gacagattag tgattgttga tagaagtacc atcatgatcc ccgcaaaata
     2701 gacagcactt acgagcacga tatgcgcaac cttttggaaa aacagtcgcg gaatgttatc
     2761 taaaaatcga ttgattttga ctcctgtatg tgtgatgaac aatagaagtc ccgtcataaa
     2821 aggtagaaca taataaaaca accgataata taatagccaa actactgcta catcttgccg
     2881 aacagacaat tgactcattc ctaagatcat caaaacgtca aaagtcccca ttccacctgg
     2941 gaccatcgtc agcattccta tcaatgtagc aatcatgaac ataggataga tcgaaagcat
     3001 cgaaatatct attctcatca acttgccgat gatcaaaaat accgcaaggg ctcccgtcca
     3061 ttgtccaaag gatgccagta gcagaccaag caagcctttt ggataaaact cgaagaacaa
     3121 tgtctttctc tttagataag caaaaaagaa aatgactggt gctaaaaaac taccgccgaa
     3181 aagccagatc caatagcttc gaaaaatact ttctgactga ataaaaaaga catcaataaa
     3241 tgtaagaaac gaccatacag acaaaccagt cagcataaat agtgcgactt ttgatacagt
     3301 ggctaaaacc atttttctat ttgtctgctt tccataaaaa ctagcccgca gacttgctcc
     3361 taccacacca ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg cagcaagaag
     3421 aaactctttt ctttccattt ttggttttcc ttgtttttct aagactctga tcgtcaccca
     3481 atcatacaac aacatcggag tgacacccag cagtccagcc agaatcatga gcaggatcgt
     3541 catgcggctt tgttgtccca tcctttgcca aatatcttgc caactcatgc cattagcaat
     3601 atttgcgact tgatttgcca caaagactaa cacggagccg aaaaaaatca gttttaaaat
     3661 cagactatgt tcctttatcc attgcagcat caagtatcgt ttatttttca cattttccca
     3721 ttcctaactt gtatactttt gtgtcatacc ccaatgttat cttatttatt ttaatttttc
     3781 cactttagac ctgttctttg cttgcgatga tagaaaaaga aagatatcct aatgctaaag
     3841 gagaggataa catgaaagta ggatttatcg gcacagggaa tatggcacaa gctattattg
     3901 atggtttatt aaaaaaagga acagtctcga aagaagaggt gttcgtttcc agtggacatt
     3961 ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa agtaatcagg
     4021 aagttgcatc acaatcagat atcgttcttt tagccgtgaa accaaaaatc attgaaaaag
     4081 tattggaaga actaagagaa tatagccaga aagtcttatt catttctatt gcttcaggaa
     4141 aatcattaat agatttagaa acagcgcttg aagatcctca ggcctccatc gtacgtgtga
     4201 tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa aatgaagctg
     4261 ccacggagga acagcttcag caagctacga tgatttttga agcaatcggc agcgtctatc
     4321 ctttagcaga atcagatttc agtacattca ttggccttgc tggcagctca cccgcattcg
     4381 tttacatgct gatcgatgcg atgggacgga ctggtgtact gaatggtttg tccaaacaga
     4441 cagccactga gattgctgca aaagcaatta tgggttcttg tcaaaaattc ctttcaagtg
     4501 ataaaaatcc atgggaactt gtagaccaag tttcttctcc tggcggtaca actgttgcag
     4561 gtgtagttgc cttagaagaa gcaggattca tcccagcaat catcaaagga atcaatacga
     4621 cgatcgagaa agatcttgag atgttgaata aaaaataatg atagcaatgt tttttcgtgc
     4681 cttacaaaaa aagaagagct gtggcttttg ccgcggctct tcttttgtat tttttattat
     4741 ttagcataat cagttgcacg agtttcccga ataacgatta ctttgatgtg tccaggataa
     4801 tccagttctt cttcgattcg tttacgaata tcacgtacta aacgaacagc gtctaaatcc
     4861 gagatttctt ctggcttgac cattacccga acttcgcgtc cagcttgaac tgcgtaactt
     4921 gattctacac cggcgaagct gttcgaaata ttttccagac ttcttaaacg acggatgtag
     4981 ttttctaatg attcacttcg cgcaccaggt cttgctgctg agagcgcatc tgctgcagct
     5041 acaaggacag agataacaga agtagcttcc acatctccat gatgagaagc gatcgcgttg
     5101 attactactg ggttttcttt gtacttggca gctaattccg caccgatctc aacgtgagat
     5161 ccttcaatct catggtcaag tgctttccca atatcatgga gcaacccggc acgttttgct
     5221 aattggatat cttcgccaag ctctgctgct aagataccag ctaattttgc aacctcgacc
     5281 gaatgcttca agacgttttg tccataacta gttctgaatc gcagacgtcc gatgatcttg
     5341 attagatctg gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc cccgtattcg
     5401 cgaatacgtt catccatttc tttacgagat ttctcgacca tttcttcgat acgcgctgga
     5461 tggattcgtc catcttgtat cagtttttcc aaggtcatcc gtgcgatttc tcttcggatt
     5521 ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat cgatgattaa atcgatacca
     5581 gtcagcgtct caagcgttcg aatattacga ccttctcgtc caatgatacg acctttcatt
     5641 tcatcattcg gaagggtaac gactgaaaca gtcgtttctg aaacttgatc tgctgcgcag
     5701 cgttgaatcg ctaatgataa taagtttttc gctttgcgat ctgcttcttc tttggcacgc
     5761 tgttcagatt cttttaccat tagagttaac tcatgattca gctcttcttc agtcgatttc
     5821 atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag ttctttttgt
     5881 tgatcttcaa tcattttttc aacgtctttt tctcgctctt caattaattg ctgttttgaa
     5941 ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt tttctaacga gtcatcttta
     6001 cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt gagattttaa ttctaactta
     6061 ctttctctca gctcactttc aacttcagaa cggtatttct gattttcttc cttagcttct
     6121 aacaatgctt ctttttttag agtttctgct tctttttttg cactatcgat gattccagca
     6181 gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg actttgcgac cataaaccct
     6241 aaaccaagac cgacaattaa accgatgata gcgaggagaa tgttgaatac cataaaatcc
     6301 acctccatac tatctttttc atttttatca tctatacagc ttctatgata aattattcta
     6361 aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa ctttattcta
     6421 atgtttgtac cccgtagtgt caactttaaa taaacaaaga aacggctttc tacttattca
     6481 ttaaacgatg gtttttaaaa taaatgacac tagtttcata taggtaaacc tttaaattga
     6541 gaaacttttc tattaaatta ttctttgtag atgaacagat atttttattt tatgtaccca
     6601 ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca ggtcaaggag
     6661 agtacctact cttcagacca gccgtttttg tttatcataa tttttattct tctactaaag
     6721 gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca ataccataag
     6781 ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc atatagattt
     6841 tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa gcaccgcttt
     6901 tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt gagatcccta
     6961 aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg ttttttacga
     7021 ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt tctgcacgac
     7081 gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg
     7141 gattcccaaa catcacgcct actttttcac ggatttggtt gatgaaaata gcgattgttt
     7201 ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa cgtgcttgta
     7261 acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact aaagcagcaa
     7321 ctgaatcgac tactacgata tctacagccc cacttgatac taaagcatca gcgatctcta
     7381 gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc acacctaatt
     7441 tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc gttccgccat
     7501 ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc
     7561 catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg acatctaacg
     7621 caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc aatttcataa
     7681 tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag gctgctttac
     7741 gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac atttcattat
     7801 ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt
     7861 atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc gcattatatc
     7921 tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg tctttagatg
     7981 aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt ccagccactc
     8041 cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg gccatctgct
     8101 ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca atctccagaa
     8161 aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact tcagatacac
     8221 cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc gctgctgtta
     8281 cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa ttatcatcgc
     8341 cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat gctaaaagag
     8401 cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc acttcatttg
     8461 gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct tttagctcag
     8521 tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt agtttttctt
     8581 cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt ggtttcaact
     8641 cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa aaagtcccta
     8701 aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg gactgttgca
     8761 gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat tttttgtatc
     8821 cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct tttgtcaggt
     8881 catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt gtttcagcta
     8941 gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga taataaactt
     9001 cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc acgacttgtc
     9061 ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc cgctccctta
     9121 tatagtatac gctgtatttt tatatttttt ta
//
LOCUS       DAZUKR010000108         8781 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000108 DAZUKR010000000
VERSION     DAZUKR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.110"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..474)
                     /gene="smpB"
                     /locus_tag="ACG89C_002193"
     CDS             complement(10..474)
                     /gene="smpB"
                     /locus_tag="ACG89C_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4900716.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(496..2853)
                     /gene="rnr"
                     /locus_tag="ACG89C_002194"
     CDS             complement(496..2853)
                     /gene="rnr"
                     /locus_tag="ACG89C_002194"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4900717.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(2856..3611)
                     /locus_tag="ACG89C_002195"
     CDS             complement(2856..3611)
                     /locus_tag="ACG89C_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4900718.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(3714..3950)
                     /gene="secG"
                     /locus_tag="ACG89C_002196"
     CDS             complement(3714..3950)
                     /gene="secG"
                     /locus_tag="ACG89C_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4900719.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(4068..4346)
                     /locus_tag="ACG89C_002197"
     CDS             complement(4068..4346)
                     /locus_tag="ACG89C_002197"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900720.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(4459..5427)
                     /locus_tag="ACG89C_002198"
     CDS             complement(4459..5427)
                     /locus_tag="ACG89C_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4900721.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      5642..5686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5710..6684
                     /locus_tag="ACG89C_002199"
     CDS             5710..6684
                     /locus_tag="ACG89C_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS4900722.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            6689..7264
                     /locus_tag="ACG89C_002200"
     CDS             6689..7264
                     /locus_tag="ACG89C_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS4900723.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(7331..8470)
                     /locus_tag="ACG89C_002201"
     CDS             complement(7331..8470)
                     /locus_tag="ACG89C_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS4900724.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         2342 a         1892 c         1570 g         2977 t
ORIGIN      
        1 ataatgaaat tatctagaga aattttttaa tgttcgatca atttgtcgat cgatatcttt
       61 tcgtttcaaa tcttcccgtt tatcataaga ttttttccct ttcgccagac caatcaatac
      121 cttggcatag ccgttacgaa tatagacttt taaaggaaca acagtgattc cagtattttt
      181 caattcattt tcaagccgga tgatttgttt tttgtgtaat aataactttc tcgtgcgcaa
      241 cggatcatga ttaaaaatat ttccttgttc ataaggactg atatgaacat tatgcaagaa
      301 agcttctccg ttgcggacgc gaataaatcc atcttttaga ttgatccggc tgtttcgtat
      361 cgacttgatc tcggttcctt gcaagaccat ccctgcttcc atcgtgtcga tgatcgaata
      421 atcatggcga gctttcttgt tttgtgcaat taattttccc tcgccttttg gcatagctgt
      481 ttcctccaac tcattttatt ttttcttttt cttcttttta acacctttgt aaaatggctt
      541 cttccctttt ttcttaccga acgtttgctt actatcgcta tatcgtttgt tcttcttgcc
      601 atttctttct gggcgttttc ctgcttgttt tccttcgcgt tttcctgatt ttccatttcg
      661 tgtttttggc cgttccaacg ctgtaacctc ttctgcggaa acaagttcaa aatctactgc
      721 tcgttcatct ggatcagctt tgactacttt tactcgcact ttctgtccga ttttaaaagt
      781 cagaccagtt ctttcaccta caagagctaa atgattttca acaaaatgga agtagtcctg
      841 tttcaattca ttgatatgga tcaatccctc tactgtattc ggcagttcga taaacaggcc
      901 gaatttcaca acagagctga tgatcccatc gaattcttcc cccactttgt ccatcatgaa
      961 ttctgctttc ttcatggaat ctacttcacg ttctgtttct actgcacgac gttccatctt
     1021 agaactatga tctgcaattt caggaagttc cgtagcccac gaactttgat tgctttcact
     1081 tttatcttca ctgtaagaac ggatcagacg atggacgatt agatcgggat agcgtcgaat
     1141 aggagatgta aagtgtgtgt agtaatctgc cgctaagcca taatgcccaa aattgtcttc
     1201 tgaatagcgt gcttgttgca tgcttcttag caacatagta ttgataactg cagcttctgg
     1261 tttgtcttct actccttcga tcacatgctg taaatctttt ggtgtgatcg ttcctttcgt
     1321 tcctcggacc agaatgccca aagctgaagc aaaatcaaag aagcgctgca ttttttcttc
     1381 ttttggctgt tcatggattc ggtaaataaa cggcagattt aatttattat aatgttcagc
     1441 taccgtttca ttggccgcaa gcatgaaaga ttcgatcaag cgttcaccaa ttcctctgct
     1501 tcttagctgg atatcatttg ggtgtccttc agcatcaaca aggatttttg cttcgcgatc
     1561 ctcaaaagaa atcgcgccac gtttgattcg catcgtttca aggattcgat gcaattcacc
     1621 catttcttgg aacataggta cgagctgagc atagcgttgc atcgtttctt cgtcttgatc
     1681 ttctaagatt tgattgacag ctgtgtaggt catccgttct gttgtttgga tcacacttgg
     1741 gaaaatatca tgagagacta cttgtccatt tggatcaatc tccatttcgc aactcatggt
     1801 cagccttgga acatggggat tcagtgaaca gataccattc gataaacgtt gcgggatcat
     1861 cggaatcact cgatcagtca aatatacact tgtccctctt tcatatgctt ctctgtccaa
     1921 ttgactgcct tgtgtcacat agtaagaaac atctgcaatg tgtactccaa gaaaataatt
     1981 accgttatct aattttttaa ccgtaactgc atcatctaga tcttttgcat cttctccatc
     2041 aatcgtaaca atgagctgat ttcttaaatc tctacgtcca gttatatcct tttcgctgat
     2101 ttgatctgga acttcatctg cttcagacaa tacatcatca ggaaatttcg tagggatacc
     2161 attagccacg acaattgaga ggatatccat gccagggtca ttcttatgac caatcacttg
     2221 cttgattatc ccttctaaac tagtcgcata ccctttttca gggtaatggg tcagttcgat
     2281 cagcacaata cttccgtcaa ctggacgaac tccctctgct gctgcatata ctttaaattg
     2341 gttgagcttt ctatctttcg ggataacaac gccgtaaaga tccgtttctt gtatttcttc
     2401 ttctgaataa gcaacgaatt cccctgctaa ctgagtagtc gcccggtctc gaatctcgac
     2461 tactttcccc tctgctccgc gatccatatc tgtatttgct cgcttcatga tatcgattgc
     2521 tacggtgtct ccatccattg caaaaccagt cgcttctttt gggatataga tgtcatcttc
     2581 ttcgggatca atcgttacaa atccaaatcc tcgttcattt gctcgaaatg ttccttcgat
     2641 caagattggt tttaaaggaa gtttcacttt tcctttttta ttgaaaacta tactctgttc
     2701 tctttccatt tgagcaatcg tttgtactag taatttaaaa tcatcgcttt tttggaaccc
     2761 taaaccttca gcgatttctt ccatagaaaa actgcgtttg cgactgcttt ccatgaaaaa
     2821 gagaattttc tcttttatcg tttcttttgt catatttatt cctcattcca aggtaatttt
     2881 tctaaaaatt ccaagacgtc tttttctaat tcgcgtcttg ctgtaccaac tgtaatcaca
     2941 tgaccacttt caggatacca tcgaagtgtc actcgctgac ggctcaactt gcgtgcagtt
     3001 tcaaatacgc cattagggtc gatcatttca tctttcccag cttgagctaa aaatatcggc
     3061 tgttggatat ctgacaacct actttctacg attgctgcct gatcttgtat atccattaat
     3121 tgttgttcag ctaacgagcg gtaagcaact gctttttcat tgatttcttt ctctgattta
     3181 cctgctgtct tcagcacttg acgtacgtat ttttcaaagt tttcaggcac gtttgttttt
     3241 acaggagaca aaggcgaaca gaaaaaaccg ccgccaatca ctgattcttc ttctagtgct
     3301 cgtacagcaa agatgccgcc cattgataat cctaatacgg caatatcaga aaactgctca
     3361 gactgtaaaa aatggatggc ttttttagtg tctgcccacc agctttctgc tttttcttgc
     3421 aaaatatcat atggctccat tgttccgtgt cctttaaaaa gcggcgcata aactgtataa
     3481 tctgcttttt ccaaaaatcg tgcgagcatt cgaacatcgt ttggacttcc tgagtaagcg
     3541 tgaagcagca aaaccgctcg ctttccatgt tttgcataaa ggggttttgg taattgtctc
     3601 actcgtctca cctctttcgc ctcattttac catagtttct ttcataactt cttcttaagc
     3661 gttctctttg caataaaaaa agccgcccgc cgttttgcga gaggcttatt caactacttc
     3721 gaagataaat atgctaggac aaataataag atcatccaga tagctcccat aactgcagtt
     3781 gatcgctgca tgacagcttc aaagccgcga gctttttgtt tgccgaatag ttgatccgca
     3841 ccgccggtaa atgcactcgc tgcactattt tgcttgcttg gctgcatcat aatcgcaatg
     3901 accataacaa tcgataaaac gatcacgatt cccaaaatta aattatacat atcgcagcct
     3961 ccttcccaaa agatatcgtc tctttaactt tatcatagat aaaagtccgt gacaactcta
     4021 tataaatgga ataacagctg aaagttcaaa actttcagct gttattctta tgactgacgt
     4081 aagacgtctt gatattctgg tttcttttca aactcttttt ttgcataagg gcataaagga
     4141 atgatctttt tcccgtctcg tcttgctaat tctactccag tatacactag cttttcagct
     4201 aacttttgac cacgatatgc tggatctaca aatgtatggt caatgatcat gatgtctggg
     4261 cccgcatctg accatgtcat ttctccgatc tcttgttgtt catcattgaa taaagcaaga
     4321 cgattttttt cttctttgat ttccatatgc cgcactcctc aaataactga tattttttta
     4381 ttcctacctt attttagcag tctcctgatc tataaacaat tatttatcct cgaattactt
     4441 acaaaaaaga agagaaaatt aattcatcgt tattctttgt tcaataaatg aaaaaatcac
     4501 tgtccaatag gttttcggat ctttcatcga tgcttccaag tgttctgctt ctggaacaag
     4561 cagcagtgct ttatcccctg ccgtagcatt ttgaatcgtg tacgctgctt ctgtagggac
     4621 aaaatgatct ctttcaccat gaatgaccaa taaaggcaga tggttgctgc caagctgtct
     4681 cgtagctgat gcttgcttta aggagtaacc ggcgtatttc tgggcataat gattcgcata
     4741 tctcatgacc atttttttag gaaagacagt gattttactt aacatatatc ggaattctgc
     4801 ataaacagaa acatatccgc tgtctaaaat aaatcctttg accgctgatg gaagtttttc
     4861 accacttgcc atcatgatcg tagaagctcc cattgatccg ccgtgtaaaa taatggacgc
     4921 attcggctgt tcatctaaga tgagttggat ccaggcaatt aaatctagcc gatctagcca
     4981 tcccattccg atgatttctc cttcactttc tccgtgcgca cgtaaatcag gtacaagaac
     5041 gttgaatcct ttctctgcat atcttttacc tatatgagac atgtctcttt tccctgtgct
     5101 tcggtaatca tgtacgcaaa tgacccattt attctgctta tttggatgga tgaacatctg
     5161 accagctagt ttcaacccgt cataactttt gattgttttg ggtgttccct gctcccaaaa
     5221 gctttcacac tcttcttcta atttttgtcg ttttttttct actgacgtga ctgcagcagg
     5281 gtccatcaaa cgatgaccag tgtcttcata ccagtcatat cctctctgta ctcccgctct
     5341 tactaatttc tttgcataaa aataagaaag aaaatggtaa ctgatcccta taattatcag
     5401 aataagaatg aaataaaaaa taaccattgt acacccgccg ctttctgcca aactataatc
     5461 taactattat ccaatgtacc gatccatagg attctttatt ttcggcacac gtaaattcca
     5521 tttctttttc attgtatcat ttacttaaag ttctcctcaa tagatttact tgtaagagag
     5581 catttacatt cgcaataaaa gccagtatac taatttcgtg cattataatc aagacgctcg
     5641 tcttggactg gttcggaaac ttcccagaat aaaaaactaa gtatctctaa agaaaggaga
     5701 ttggaaataa tgcgaaaagt aattattgat tgcgatccag ggatcgacga cactttagct
     5761 ttatcattag ctgtcaaatc ccctgatatc gaagtagttg ctattaccgt agtttgtggg
     5821 aatgtccctg cagatatagg tacgcagaat gtgctgaaat gcctggaacg ctgtgatcgt
     5881 ttagacattc ctgtttatca aggaatggaa aaacctttga agcaaccttt catcagtgca
     5941 caagacactc atggtatgga tggtttaggg gaaacaaatt tcccgatgat cttgagaaaa
     6001 caggcagaac ctctccatgc cgttgatttt ttggctgatt atttcaaaga aaagacaaat
     6061 acatcagtca ttgctttggg tcctttgacc aatatcgctt ttgctttgaa agtcaaccca
     6121 aatattggga aacatatgga tcgttttgtc tcgatgggtg gaacttacaa aagccatggt
     6181 aactgttcgc cagttgctga atacaattac tggtgtgatc ctgaagcagc ttcttatgta
     6241 ttcgagaatc tcaaacaaac gatagaaatg gttgggttag atgtgacaag agaaatcgtc
     6301 ttgacaccga ctatcctcga atactgctgt caaatgaatc ctgaagaggg agagtatcta
     6361 aaagcgatca ctcgttttta ttttgacttc cactggaaac aagaaagaat tttaggatgt
     6421 gtcatcaatg atccgttagc tgttgcttat tttatcgagg gggccatctg tgaaggtttc
     6481 aaaagcttca ctgctgtgga aactcaagga ataagtcgtg gacaaacatt ggttgatcgt
     6541 tatgaatttt ggcagaaacc tgctaacagt aaaattatga caaaagtaga tactcgtctt
     6601 tttttccgaa agtttttaac cgttctttta aatgcacaag aagaaacgat tatgaaagat
     6661 ttggaaaggt taaagatggg ctaacaaaat gaataaaaac aaaatggcaa ctcgcgtact
     6721 tacagtgatg gctttgtgta ttgggatcaa ttatattgga ggaacgattg ctttatggct
     6781 tcgtttgcca atctatctcg attctatcgg aacgattttt gctggtgcat tgcttgggcc
     6841 tgtacctgga atgctgacag gattgtccag cggtatttta agtggagtga ctacagatat
     6901 tttttctctg tattattcgc caatccaaat tctcacaggc ttgcttgctg gattgatttt
     6961 gccaagatat ctcggcaaag ataaaaacag cttgaaactg ttattactcg ctttgatttt
     7021 gtcggtacct ggaacgatcg tttcttcgat cattaccgtt cagttatttg gcgggatcac
     7081 ctcctcagga tccagtatga tcgttcagct attacatggg ttaggtttaa gtcagacaac
     7141 aagtgtgatc ttggtacagg caggtacaga ctaccttgat cgtttgttgt ctatcgtcgt
     7201 tgtttcatta gtcatcacta tgttgccaca acgcttgata tattcaaaaa gaaaaatagg
     7261 ctgaataaac agagctggat tagcaaaata atgctaatct ggctctgttt tttcatttat
     7321 taaaaaaatc ttacgctaag taggataaaa taattccttg atatttttca atcatatcca
     7381 aataattatc gatttcaaca tactcatcta cttgatgagg caaagtaacg acaccaggcc
     7441 caaagacgac aaagtcgaag gaatggttag ctttcgtaaa ttcggctgca tccgttgttg
     7501 ctgccgctcc aactaaaggt aatggctggc tgaattgttg ctgaatgcaa tgaatcaaag
     7561 gagaatctgg atcagctttg acaggtattt tgttgtaatc gatcatcaat tccaaatgat
     7621 agtctgtctc ttgattcaat tcattgacga ttgattgcag caatgcaatg attttatcat
     7681 ttgggtattc tggaatcgat cgaatgttcc cttggagctg cgcatggctt gggatgctgt
     7741 tgacttgatt cccgccactg atcaatgtaa cattatggat tgtttttcct aggacaggat
     7801 tctcgattgt ttcagcgagg tgattcattt cagcatttgc tttcgtaatg aattcattta
     7861 ggtgattgat tgcattataa ccttggtctg gcatagagct atgcgcttct tttccgtgcg
     7921 aggtgactgt atagttgatt gatcccatgt gcgtatacat caaactgtaa ttagttggct
     7981 ctccgatgat caaggcatct aagtcatcta catagcctgc tttcgtgagc tgttccccac
     8041 ctagttcacc cacttcttct ccgacagtgg ctaataattt gactgttccg ttaaatggtt
     8101 ttcctgactc tttcagttcg atcatggcaa tcaccattgc tgccaagcca ctcttcatat
     8161 ctgtagcacc acgtccgtac aaacggttcc catggatttc tgctgcaaag ggtgcatagg
     8221 tccaagacga ttcatcacca gccgccacga catccatatg cccagatagg cctaacactt
     8281 ttcctgattg gcccttttgg taacgagcaa tcaaattatc tctaccatct ctgtaagaaa
     8341 caatctctcc tgttatgtca tgctttgcca aaagcttatt caaataggct gctacttcac
     8401 cttcgtttcc attcactgat ttaattcgga tgatctcttg taaaatcgcg attttttctt
     8461 cttttttcat ctaatcctct cccttgccat ttctttttat tgtatttgag tgtgctcgaa
     8521 ttttcaaata ggagcaacca aagtaaaaat gaagaaagag agccacttta tgaaaaatat
     8581 gctgctactt ccctcctatt ttatttatac gagaggactt gctcatagat tcctattcta
     8641 ctgttctatc cttaaaagct cttatatgat gctagttatt gttagctttc ctttttcaag
     8701 gatgatcttt tcttgtacca ttctttgtaa acatcatata cagaagctag ttgcaaaaaa
     8761 aactctctac ctgctgatat t
//
LOCUS       DAZUKR010000109         8675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000109 DAZUKR010000000
VERSION     DAZUKR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.111"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACG89C_002202"
     CDS             <1..1101
                     /locus_tag="ACG89C_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4900725.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACG89C_002203"
     CDS             complement(1280..1615)
                     /locus_tag="ACG89C_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4900726.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACG89C_002204"
     CDS             complement(1599..1862)
                     /locus_tag="ACG89C_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4900727.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACG89C_002205"
     CDS             complement(2322..3125)
                     /locus_tag="ACG89C_002205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4900728.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACG89C_002206"
     CDS             complement(3103..4227)
                     /locus_tag="ACG89C_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900729.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACG89C_002207"
     CDS             complement(4243..5058)
                     /locus_tag="ACG89C_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4900730.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACG89C_002208"
     CDS             complement(5048..5920)
                     /locus_tag="ACG89C_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4900731.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACG89C_002209"
     CDS             complement(6005..7294)
                     /locus_tag="ACG89C_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4900732.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACG89C_002210"
     CDS             complement(7438..7950)
                     /locus_tag="ACG89C_002210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900733.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACG89C_002211"
     CDS             complement(8017..8160)
                     /locus_tag="ACG89C_002211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4900734.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAZUKR010000110         8545 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000110 DAZUKR010000000
VERSION     DAZUKR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.112"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..749
                     /locus_tag="ACG89C_002212"
     CDS             <1..749
                     /locus_tag="ACG89C_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HFS4900735.1"
                     /translation="EVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIGVILTINTGW
                     LLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVNTFGSDVYDL
                     TVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIENHRYTLVFY
                     IGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLDQFKQTLPQP
                     ISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1563..2909)
                     /gene="gor"
                     /locus_tag="ACG89C_002213"
     CDS             complement(1563..2909)
                     /gene="gor"
                     /locus_tag="ACG89C_002213"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4900736.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3043..4296)
                     /locus_tag="ACG89C_002214"
     CDS             complement(3043..4296)
                     /locus_tag="ACG89C_002214"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4900737.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4302..5225)
                     /locus_tag="ACG89C_002215"
     CDS             complement(4302..5225)
                     /locus_tag="ACG89C_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4900738.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5446..7938)
                     /locus_tag="ACG89C_002216"
     CDS             complement(5446..7938)
                     /locus_tag="ACG89C_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS4900739.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7945..8412)
                     /locus_tag="ACG89C_002217"
     CDS             complement(7945..8412)
                     /locus_tag="ACG89C_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS4900740.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2344 a         1811 c         1417 g         2973 t
ORIGIN      
        1 ctgaagtata taagtatgaa gaaaatgtca ttggccgcag aaatctatct cctgtattga
       61 ttcgaaacat gattttagct atgattgcat ttgtttttgt tatcggcgtt attctaacga
      121 tcaatacggg ttggctttta cttttgatgg gatttatctg ttgttttatt ggagtattct
      181 atacattcgg accgattcct ttatctcgaa tgcctttagg ggaaattttc agtggggtga
      241 ctatgggcct tggaattttt gccatggtcg tttatgtcaa tacattcggt tcagatgtct
      301 atgatctgac agtcaacttt tcatctggac aatttgattt ggtaggaaat ctttgggcta
      361 tagcagcaat cgtattagct tccttaccct tggtgtttac gattgccaat atcatgttgg
      421 ctaataattt gcgggatctt gaccgtgata ttgaaaatca tcgctataca ctagtttttt
      481 atatcggacg tcctgcggga acagtcttat tccagctatt gatgtatgct tgctatttcg
      541 tgattttaat ggggttgttg agcggtatct atgaatggcc gatcttggtc accttcttga
      601 ccttgccaat tgtgaagaag catttggatc aattcaagca aacgttaccc cagcctatca
      661 gtttcagcta ttccatcaaa aatatggtct tattcaatgc cagttatgta ttggggctat
      721 tactaacaat cattatgcaa gccatttaac agcatgaaga ggttgtgaaa aaactgccct
      781 gcatcaagta ataagaacag ccaaataaaa atagctcctt atatttttcg tttggttggg
      841 cttattaccg cagatgcaag cttttgaaca ccgtttatca ggaggttgtg aaaaagtcgc
      901 ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
      961 tggcttaata ccgagaaact ggcttttgaa caccgtttat cagaggttgt aaaatcagtg
     1021 ttttgtcctg agtattagag aaacaaatgg aaaaatagtt cctcatattt tgccgttttt
     1081 tgagctaata ccgaaggaca gctgattgaa cactatttgt tcggaaaaag tgactaacta
     1141 tgacacaatt tttttcacag tccctttttt cacttgattt gattcgtacc agcgaacttg
     1201 acgagaactt tcttggtttg gtaactatat ggtattcttt taacatcctc gtttgcttct
     1261 tctaatttat ttatacaaag tcacactttt ttaaacatct gttcttcgat acatttctat
     1321 ttcttttaaa catcgatacc aatattttga acttaagagt gcataccaaa tggattcatt
     1381 tactcttcgt cagttacgaa gataatcaaa atctccaact tccctactta tttcgttttg
     1441 agaattatcg aagcaatctt acataaataa ctaaaaaaca aaaaaccaga aaatcagtat
     1501 actgattttc tggttttaca tggtttgttc aataaaaagc tgttggaact gttaatatta
     1561 aattatcgca tcgttacgaa ttcttccgct cctgttggat ggatggctac tgtattgtca
     1621 aagtcttctt tagttgcacc catcttgata gccacggcaa aaccttgcag catctcatcg
     1681 acaccaacac caatagcatg taagccgacg attctttctt cttttccgac acagatcaat
     1741 ttcatttcgc atttttggcg ataatcattt aatgcaaagt acattggggt aaagcgtgaa
     1801 cgataaactt tcaattcatt ttcgccatat ttttccaatg cttcttcttc tgtcaaacca
     1861 attgttgcaa ttggtggatg cgtaaagacg actgtcggaa caagtttata gtcaagataa
     1921 gaatctttct ttccattaaa taaacgttct gataaacgtc taccagctgc gattgcaaca
     1981 ggagtcagat ctaatttacc gatcacatca ccaacagcaa aaatatgttc atccgttgta
     2041 ttttggtatt tatctacttt gataaaccct tttttgtcca attccacatt tgtattttcc
     2101 agacctaatt tttcggtatt cggtacacgt ccaccggcaa aaagtacaca atcaccagtt
     2161 aaggtttcac cattttcaaa tagcaccgta tattcgtcat tgtttttagt aatttcttta
     2221 gcaatatgat gtgcgtaagt ttttattcca gcttcttggt acatttggat caaattgtcc
     2281 gaaagcattt tatcaaacgt tctaagtggt cgttcttttc gatatgccca catgacttca
     2341 ctacctaaac cacgaaatac acctgaaagt tcagccgcaa tgtatccagc acctaaaacg
     2401 attgctctct taggtaactc tgttaattca aaaaatccat ttgaatcaat tgcgtattct
     2461 ccaccaggaa tggtcatttt acttgcacgg cctccagtag cgatcaagat tttaggagca
     2521 cgataggttt caccgtttac ttcgatcgtt tgactatcga taaaagtagc gtaacctttt
     2581 atagcctcga catgattgct atctaatcct ctttgataag caccgtgtag aaaatcaata
     2641 tatttttctc gtctttctac tagaccggcg aaatcaacat tttttaaatc tgcttggata
     2701 ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg tacttgcttg ccacataact
     2761 tttttaggta cacaaccgac gttgacacat gttccgccca attcattcgc ttcaatcagc
     2821 aaaacttttg cgccatgcat ccctgctctg tttgctgaag caataccgcc agagccgcca
     2881 ccaattacga tgtaatcata tatttgcatt ttgttcctcc ttattgaaga gatcaatcaa
     2941 aacctttatt taacgatcct ttttctacag aaaacaagtg taacactttt cttgaatttt
     3001 gtatgcgaag atgcttaaaa cagggatata tcagcttttt ccttatttac gttttcggat
     3061 catatctcca ggtttcaccg gctgtgcgac tggcatagtt aaaatcatca ttggatttgg
     3121 tgctcggtca atggattcgc cttcttcatt atgcataact tcaacggttt gactgaaatg
     3181 actgaatcct ggaccataaa attcgatttc atctcctaca gaaaacaagt ttctctgacg
     3241 tatcgtagca atctgtgttg cctcatcata agataatact tcaccgacaa atttatatac
     3301 tgggatttta cgacgttcac cgaataattg ttcgttttca gttggtgtat tgtagtaaaa
     3361 acctgtagct aattctcgtt gtgctacttt ccataactcg tcgatccatt cttgcttgca
     3421 gacatagttt tcaggatctt ccatataact gtctacagcc gctttataga catttgacac
     3481 cgtagataca taatggatcg atttcattct tccttcgatc ttgaagctgt ctacgccgtt
     3541 ttcaatcaat tctggaatat gttcgatcat tgacatatcc actgcactca tcgagaattc
     3601 ttcttccact tcgcctgtat cagttaaact gtttcgttct tgaccaaaag gcatatcgaa
     3661 caatccatac ttccagcgac aagattgaga acatccgcca cggtttgcat cccgcatcga
     3721 catatggttg gacaatgtgc atcgaccaga ataagagata cacatggcac catggataaa
     3781 tgcttcgatt tcggtatccg tatttttacg aatctctgcc acttcctcca ttgaaacttc
     3841 acgagctaaa acgacacgtt ccaacccttc ttctttccag aattccaatg tctcaaaatt
     3901 agttgctgaa gcttgagtgg ataaatggat cggcagacct ggggcttctg tcgcacaaat
     3961 ttcgatcaaa gctggatcag aaacaatcac tgcagaaata ccgacatcac gcaaactgcg
     4021 gaaaaattcc cctgctccat cttcatttcc ttcatgggtg accatattag cagcaacgta
     4081 taccttcgca tcatgttctt ttgcaaaagc aactccttca gccatttctt cataagtaaa
     4141 attacctgca cggcttctta aaccatatgc attcccgcca atatatactg catctgctcc
     4201 ataatggatc gcggttttta acttttccag tgttcctgct ggcgctagga tttctggtcg
     4261 tttcaatttt ctttttactg tcatttcttt catcatactc cttatttgat agcagaagga
     4321 tcaatataaa agaatcctgt atctaattca cggttttcag gatgcaattt actgatttcg
     4381 ctagtcattt tttccgcttt ttcttgatcc cagctacctt ctaaaatttg tttttttgct
     4441 tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg gagtaaataa tccgtccaat
     4501 ttccatgtac agtaatgatg ctgataaagt tcattcaatt cattcgagag attcagatca
     4561 ttgctggcaa aaatatgtgt gccatgacta tcttcataaa tagaataatg tgtctcttct
     4621 tttttaggtt ccgagataaa aagacctctt tcacgatctt tttgttcgtc ttgtttcgta
     4681 tagttatagt aattctgtag cagtggacgt ttggattgat ggatgcatgt agcaccatac
     4741 acaagggttt ctacaggaat atcgagtttt tcttccatcg ccttcatctc ttcaaaagga
     4801 acttcacgag caagtactgc tccaactgcg ccttttttcg cccagaaatt gatttgacgt
     4861 gcgctcgtta ctaatgtttc agcatcataa atatacggta tacgcaattc aggatttttt
     4921 ttcatcgtat aaataatacc tgtatcacca actgtgatca catctacgtt tatttctttt
     4981 aaaaaagcca aatattcagg tacatttttc attttttcag gatgaatgat cccatttaca
     5041 gcaatattcg cttttttacc atacttgtgt gctaattcta ccaattgtcg ctgttcttct
     5101 cgtgtaaatg aagcaggcag acgcaatcca aattgctctt caccgaaata tagtgtatct
     5161 gcacctgcag caagcaactg ttctgcttgt tcaatagttt ctactgtggt tatgagttcg
     5221 atcataattt tctcctccta caacttcata atagtagcat agctctaaaa actttccaat
     5281 acttcttcta tttaaagtcg gatttttgaa aattatttca caaaaaatca aaataaagtt
     5341 cgatatattc tgtccaaata taaaaagaga ttgtgacaaa aatcttgtca caacctctta
     5401 atcaagtctt actattataa aaattcagct acacaggcca agtaactata gggaacctac
     5461 tgtttccttt ttaggtccgc gaacattcaa tgtgatctta ccttttgaag cacctactgt
     5521 tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc tcgctcaatc gatcttcaat
     5581 ttctttttga agtgcgcgac ggattggacg tgcaccatat tcaggatcaa atccagcttt
     5641 cccgatcaca tcgattgcag atggtgtgat tttcacttga atatcttgtt ctttcaatcg
     5701 tttgataatc gctttgctca tgatttggac gattgtatgg atttcatctt tactcaatga
     5761 atggaagacg acagtctcat cgattcgatt caagaattca ggtcggaaag ctttctttaa
     5821 ctcttctaag atacgttttt gcatggcttt gtgatcttta gtaacatctt ggacattgaa
     5881 tccgacattt ttttcttctc gaatttgtgt cgctccgata tttgaagtca taatcatgat
     5941 tgtatttcta aagtccactt ttctaccttt agaatctgtt aaatgaccat catccaatac
     6001 ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc acttcatcta agagaatcac
     6061 tgaatatggt ttttgacgaa ttttttctgt caactgtcca ccttcatcat accctacgta
     6121 tcctggagga gacccgatta atcggcttgt actgtatttt tccataaatt ctgacatatc
     6181 aacacggatc agggcatctt cacttccaaa catgacttct gaaagtgctt tggccaattc
     6241 tgtttttccg acaccagttg gtccaaggaa catgaatgaa ccaattggac gatcaggatc
     6301 tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact gctttgacag cctcatcttg
     6361 accaacgaca cgttcatgca gaagtccttc cagttctaac aagcgttcgc tttctttttt
     6421 ctccaactgc tgtagaggga caccagtcca ctccgataca actgtagcaa catcttcttc
     6481 tgttacacga tcagaataac cttgctcctg ttttgcttca attgcagaaa ctttttgcag
     6541 ctttttacgt agtgcttttt cttgtctgcg taattgagca gcattttcaa agtcttgttt
     6601 ttggatcgct gcttcctttt cttgtactag ttcatcgatt tctagtttga tcacagttga
     6661 ttttgttaag tgatcggttt gatccaaacg gactttcgct gctgattcat cgatcaaatc
     6721 aattgcttta tcgggtagtt gacgagaagt gatataacga acagaaagat tgactgctgc
     6781 tcgtacagct tcatctgtga tctccactcc atggtgctct tcatatcttg tacgtaatcc
     6841 ttgaagaatc acttctgctt cttcaggagt tggctcatct acttgaatgc gagcaaaacg
     6901 tcgctctaat gcagaatctt tttcgatata tttttgatat tcatctaggg tcgttgctcc
     6961 gatcgtttgt aattctcctc gagctaacgc cggtttcaag atattcgaag catcaattgc
     7021 tccttctgct ccacctgcac caattaaggt atgcaattca tcaatgaata agattacttg
     7081 accatcatta tagatttcgt cgatgacctt tttcattcga tcttcaaatt caccgcgata
     7141 tttcgtacca gcaaccaaag atcccatatc aagcatcatc aggcgctttt cttgcatgtc
     7201 ttctggcact tcacctaaaa tgattttttg agctaaccct tcagcaatgg ctgttttgcc
     7261 tacaccaggt tcacccacca gtacagggtt attctttgtc cgtcggctaa gaatctggat
     7321 aagccgtttc acttctctcg aacgaccaac aactgggtcc agcttgtttt cttttgctaa
     7381 tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt ccttgcggtg tttgccgttt
     7441 atcttgtaca tttacatttc tacgacgatt cattcctttt ccagcagaat caccagctag
     7501 ccccattttt tgtttcagaa cttgacgggc ttttgaaagg ctgataccta agttgatcat
     7561 gattcgagta gccatgatac tttcatcttt taataagctc atcaaaatat gttctgttcc
     7621 aactttttga gaaccagttt tcttcacttc gctgctagct aaagcaaaaa cttgtttcat
     7681 tcttggtgaa tatggaagaa agactccttt agggtaagaa cggataccac cataaccaat
     7741 cagatgttca atttcttcat aaatttccgt ttcattcaaa tccatttcac gtaatacttt
     7801 tccagcaatt ccattttgct cgaccactaa ggctagtaaa acatgttctg aactaactga
     7861 ttgatgtttc aaccgttttg cttcttcttg tgcaatttct aatactgctt gggctctttc
     7921 agtaaaaagt tcttccatct aatctcactt cccatcttca taacttaaac gttccagaaa
     7981 tgctcggaga atccgagcac gtgtatgatc ttcatattca ttgaaattca atgtgttttt
     8041 tgcaattgca ctcaacatca agtttccttc tttttttgta attatttggt cttcataaag
     8101 tttttgaata atagcaaaag catttttctc actgattgta tcatcaaata actgatttat
     8161 ctgctcaagt aattgttttt tatctgaaat ccttactttt gcaattcgta tataaccacc
     8221 accaccacgt ttgctttcta ctagataacc acgttggatg gtaaaacggg tattaatcac
     8281 atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc atttcaatcc ttcgaatctc
     8341 gatcatttca ctattttcca gaattttctt tatatatgat tcaattaaat ccgaggtatt
     8401 ctgatgtgcc atcttattca ttccctttga ctaatattga ccttaattat aacgaatttg
     8461 ataatagaaa gaaagaagaa cgctttttgt taaataaatc ctttagaatc aacaaaaaaa
     8521 aatacggaat atactattcc gtatc
//
LOCUS       DAZUKR010000111         8494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000111 DAZUKR010000000
VERSION     DAZUKR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.113"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG89C_002218"
     CDS             complement(<1..149)
                     /locus_tag="ACG89C_002218"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900741.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG89C_002219"
     CDS             complement(593..1714)
                     /locus_tag="ACG89C_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS4900742.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG89C_002220"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG89C_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS4900743.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG89C_002221"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG89C_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS4900744.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG89C_002222"
     CDS             complement(4304..5215)
                     /locus_tag="ACG89C_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4900745.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG89C_002223"
     CDS             complement(5232..6239)
                     /locus_tag="ACG89C_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4900746.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG89C_002224"
     CDS             complement(6236..8365)
                     /locus_tag="ACG89C_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4900747.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG89C_002225"
     CDS             complement(8370..8480)
                     /locus_tag="ACG89C_002225"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900748.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZUKR010000112         8325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000112 DAZUKR010000000
VERSION     DAZUKR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.114"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..683
                     /gene="lepB"
                     /locus_tag="ACG89C_002226"
     CDS             132..683
                     /gene="lepB"
                     /locus_tag="ACG89C_002226"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4900749.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(766..1308)
                     /locus_tag="ACG89C_002227"
     CDS             complement(766..1308)
                     /locus_tag="ACG89C_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4900750.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1545..1943)
                     /locus_tag="ACG89C_002228"
     CDS             complement(1545..1943)
                     /locus_tag="ACG89C_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4900751.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2203..3063
                     /gene="ylqF"
                     /locus_tag="ACG89C_002229"
     CDS             2203..3063
                     /gene="ylqF"
                     /locus_tag="ACG89C_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS4900752.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            3056..3823
                     /locus_tag="ACG89C_002230"
     CDS             3056..3823
                     /locus_tag="ACG89C_002230"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4900753.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3879..4736
                     /gene="dprA"
                     /locus_tag="ACG89C_002231"
     CDS             3879..4736
                     /gene="dprA"
                     /locus_tag="ACG89C_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS4900754.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4858..6936
                     /gene="topA"
                     /locus_tag="ACG89C_002232"
     CDS             4858..6936
                     /gene="topA"
                     /locus_tag="ACG89C_002232"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4900755.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6905..8281
                     /gene="trmFO"
                     /locus_tag="ACG89C_002233"
     CDS             6905..8281
                     /gene="trmFO"
                     /locus_tag="ACG89C_002233"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS4900756.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2906 a         1456 c         1743 g         2220 t
ORIGIN      
        1 gatcaaaaaa gttttttgtc caatagccta attgaaagaa ttatttttat ttcagctatc
       61 tagtcgcatt tttttgatat ttttcttata ataaagaaag aatgaaaaaa agagggatgg
      121 attgatagag attgacaaaa aaacaacgct atattaatcg cttctggctt gtattcaaat
      181 acttacttgt ttctgtattc cttgcgttca tgttgcgggg atttttattc atcccggtac
      241 cagtggaagg caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt gtgatggaaa
      301 aattttcgga gatcagacga tttgatattg ttgtttttca attagcagat ggcacgattt
      361 atatcaaacg agtaatcggt cttccaggag aaaatgtcag ctaccaaaat gatcaattga
      421 agatcaatgg gaaagtagtt aaggaacctt atctaactaa aaacctgaaa tcagatcatg
      481 caaatgcatc ctatacgacg gactttacac tacaagagct aacgggacaa tccaagcttc
      541 cggaagataa ttattttgta cttggagata atcgaagagt ttcaaaagat agtcgttcgt
      601 ttggcacgat caataaaact gatattctag gtaaagcaag atttgtctat tatccacttg
      661 atgaaataaa atggatccag tgagaaacaa taaagcgtgc agtctgttga gactgcacgc
      721 tttattgttt aatccaaaat cagtttacta ccttagtacg taaaatcaaa cagctcgttt
      781 aaacactttt ctagcctcta aagttttgat aaatgaataa acaaccatta tttgaatagg
      841 gatttggatc atattcctga tgaatctacc tagaaaaatt actttgaaat ctgcaccaaa
      901 caaaacagac atcagccaag aattgataaa aaggctgact gggatcataa tcaataattg
      961 ggcagcgatg acggaaggca ggtttatctt tttatgatat aaaaataagc cattaataaa
     1021 tccaccaagg aaagccgtga taagaaaagc agcttgaaac gttcctgaac caaatatcgt
     1081 gaaatttaaa gtgttcgaca acatttcaag caaaccagcc cagatcggtc catatagata
     1141 acccataagc gccatactta aaaatccgaa tgtgatttga aaataagcag gaccaatcgc
     1201 tatcttgtat aagatgacat caagggctaa taaaagagca gaggtcgaca acaatcttgc
     1261 attcatttcg ggcaatctaa aaacgtcttt tgcaatcaaa gtgttcaaaa tattctctcc
     1321 tttgtcagtt aataataaaa ggataccata aaatcaccaa tgtgtcatga cacattggtg
     1381 atttacatac actggcatct aacaattaaa aaaagggtga gaagactgct tttataaact
     1441 gtaaaaaaga gattgtacga ataaaaaaaa tagaggcgga gaatacttat cgtatcatcc
     1501 gcctttgttt ttttgttata aaaaacgtca gattagttat ctgcttacat cttcatccat
     1561 acgggttcta atgtatttcc gtctagatct tggacttcta atgaaaacat ttcttcttca
     1621 ggcattccca tatctacttc gtggaaagta ccaccatttt cttttgcaat ttgagcaaat
     1681 ttttttactt catccgcgtt ttcaacacta aacgaaatca aagcaccact tgttttttta
     1741 ttatcagcta tttctttatc ttttataaat tttttataaa aatcatgaga gagaagcatt
     1801 atccaaaaag tgtcatccca taccattgaa ccagcttctt cagtagaaaa ttcttcattt
     1861 tttttaaaac ccagcttttc ataaaaagca acggaacgat tgacatcagc aacaggaaaa
     1921 ttaacaaaaa ccatactatt catttgtttc gtctcctttc tgttttgttc gtctatcatc
     1981 tcttatttag gaaacgctgt caaaaattta gattcatata tagaaaccaa aataaaaatt
     2041 tgcatcatca aaaaagtgaa gatgtgatag aatgaggact ggttcacttt cacttaacta
     2101 atagatggaa tttctttata ataaaaaagt gaatttatat tgaaaaagaa aaaaacaagt
     2161 aaagccgggg aatattccgg tatgaaagga atggagagaa caatgaccat ccaatggttt
     2221 ccgggacata tggcaaaagc ccgacgagaa gtttctgaaa aaatgaaata tgtggacatc
     2281 gtatttgaat tggtagacgc acgattacct ttatcttctc gtaatccaat gctagatcag
     2341 atcattcagc aaaaacctcg ccttgtttta ttgaataaag cggatctggc agatccacaa
     2401 cagacaaagc tgtggcaaga atattttcgt aaacaaggcc atcttgcatt agctatcacg
     2461 gcacaagaaa gtaaaggaat taaaggtcta gtacctgcgg ctaaagaagc attgaaagaa
     2521 aaacgggcaa gagatgctgc tcgggggatc aagcctcgcg ctattcgggc aatggtctta
     2581 ggtattccaa atgttgggaa atctacgttg atgaatcgac tagtaggcaa gaaaattgca
     2641 cagacaggaa ataagcctgg cgtaacaaaa ggtcagcagt ggcttcgttt aggtaatgag
     2701 ttggaattgt tagacacacc agggatttta tggcccaaat ttgaagatga agaaatcggc
     2761 aaaaaactag cgctgacagg cgcgatcaaa gaccagcttg tccatttaga tgatttagcg
     2821 atttacggat tagacttttt tgcccgatat tattcaggaa gaatcagcga tcgttatggt
     2881 ttatcaatcg agcaagaaga aaaattggca ccggaattac tgatggatat tacagcaaaa
     2941 cgtgggtata aagaagacta tgatcgtggc agcgaattag tcgtgcatga tattcgtcaa
     3001 ggaaaacttg gacgatacac actggatcgt tgcgaagaaa tggagcaaaa aagtgatgac
     3061 tgaatccatc caaagtatca aagaacgatt gaaaagcata cagagaatag atgatcctac
     3121 actagaatca ttaagaaatg atccacgcaa aggcgtgcag caagcactgg cttcttttga
     3181 acgacgattg aaaaaggaag cattaattaa ggaaaaatat gaagaaatgt tttgctttga
     3241 aaagcaaggg tacgcacaag gtcaccaatg gattgccggc attgatgaag taggaagagg
     3301 accattagct ggacctgtcg tggcagctgc agttatcttg ccgaaaaatg agatcatatt
     3361 agggctgaat gactccaaac aattatcaga gaaaaagcga gaacttcttt atcaacaagt
     3421 tcaagaaaaa gccctggcaa tcgggatcgg gatcgttgat cagcagacaa tcgatgatgt
     3481 gaatatttat gaagcaagta aattagcgat gatccaagca gtagaaaaat tgtccattgt
     3541 accaagctat ttgttgatcg atgcgatgac cttggatttg cctattcagc aggaaaaaat
     3601 aatcaaaggg gatgcacgat ctgtgtcgat tgctgctgca agtatcattg ctaaagtata
     3661 tcgagaccaa ttgatgaaag aatatgacgt gctttatcct ggatatggtt tctcgcataa
     3721 tgcaggttat ggaacaaagg aacacttaga aggacttgaa cgacaaggaa ttacgccaat
     3781 ccatcgtaaa agttttgcac ctgtaaaaaa atatttacaa taaaaaacag ccgcttttac
     3841 gtatcttatt agaaagagag acgaaaggcg gttttatgat gtatcaaata gaagaaaatt
     3901 tattgaaact tgcttataca aaaggaatca gtagtcaagg aaaatgggca cttgctaact
     3961 ggatgatgca gtatccttat aaagaaatcg gatttgaaga catcattaag ataggtaaaa
     4021 taaccaaaca tcgggagctg tttcgcaaaa gttggcagga aattcacgct aactggggca
     4081 aaatccaatc gaaacagtcg tttatcactt gctttgatcc caaatatccc cctcagctgc
     4141 ttcacttgac ctatccgcca attgtactgt tttataatgg tgatctatct ttactttctt
     4201 gtaacatgtt aagcgttgtt ggtggaagac acacttctgc tttagccaaa aaaacagttt
     4261 attatttact aaaaccagtg atagaagctg gatatgtgat cgtcagtggc tgcgcgaaag
     4321 ggatagatac atacggtcac caagcagcaa tctctcatgg tggacggaca atcgcagtca
     4381 ttggaacagg gatagagcaa gtctatcctg ctgaaaatcg tgacttgcaa gaggtgattg
     4441 caaatgatca tcttcttttg agtgaatatc ctcctcaatc cggcccgaaa aagcattgtt
     4501 ttccaatgag aaatcggatt atcgcagctt tgtctaaagg aacatgtgtc tttgaagcca
     4561 agaaaaaaag cggctctctg ataacagcac agcaagcatt ggatcttgga agaacagttt
     4621 tttctgttcc aggaaatatt ttaacagaac attctaccgg atgtcatcat ttgatccaag
     4681 acggtgcagt ttgcacggtt tcgggtgaag atatcttggc agaatttaaa gaataacacc
     4741 atacttttca tttgcttttc ttgacaaacg ttttctgaat ggatatgatt agctacgatt
     4801 tgtatgaatc ctcttttaat agtaggaaca cgacaatgga aaggagtcca ttcagtaatg
     4861 gcgtataaat atttagtgat tgtagaatca cctgcaaaag ccaagacaat tgagaaatat
     4921 cttggacgaa actataaagt aatggccagt gttggccata taagagattt accgaaaagt
     4981 aaaatgggta tcgattttga aaacaattat gaaccccatt atatttctat acgcggaaaa
     5041 ggcgatgtca tcaaaagcct gaaagccgca gcaaaaaaag ctcaaaaagt ttacttggca
     5101 agtgacccgg atagagaagg agaagcgatt gcttggcatt tagcgtacct acttgggttg
     5161 gatctgaaag aaaaaaatcg ggtggtcttc aatgaaatca caaaagacgc agtcaaagca
     5221 gcttttaagg aaccaagaac gatcgatgta gatttagtag atgcacagca agctcgtcgt
     5281 accttagaca gaatcgttgg ttattcgatc agtcctattc tctggcgtaa ggtcaagaaa
     5341 gggttaagtg caggacgtgt ccaatctgtc gctttaaaaa ttattattga ccgtgaaaaa
     5401 gagatccgag aatttgttcc agaagaatat tggagcatcg acggtaattt taaaaaagct
     5461 cgcaagaaat tcaaagcaaa tttctgggga atcgacggta agaaaaagaa attaccagat
     5521 gcacaaagtg taaaagaagt cactgctaga attgatggaa cagacttcga agttaaaaaa
     5581 gtagaaaaga aagagcgcaa aagaaaccct gcgaatcctt tcactacaag tagcctgcaa
     5641 caagaagctg cccgtaagtt aaactttcga acaagaaaaa ccatgatggt agcacagcaa
     5701 ttatatgaag gaatcacttt gggtaaacaa ggaacagtcg gcttgattac atacatgcgt
     5761 actgattcta cccggattgc cgatacagca aaagcagagg ccgcagcatt tattgaagat
     5821 acgtatggaa aagaattttc tagacatgat caccgaaaga tcaaaaatgc tcaaggcgca
     5881 caagatgccc atgaagctgt gcgtccaacg agtgtgcttc gtacaccaga tgaactcaaa
     5941 caatatttag ataaagatca attgaaactt tatacattga tctggtcacg ttttgtggct
     6001 agtcagatga caccagctat tttagataca atgaaagtga cattagaaca aaatggtgtc
     6061 atcttcattg cgaacggttc aaaaatcaaa ttcaaaggat tcatgcaagt gtatgtggaa
     6121 ggtagagatg acggcaaaga agagaaagaa aatattcttc cagaacttga agaaggcgat
     6181 gtagtccaat ctgttgatat tgaaccaaaa caacatttta ctcaaccacc agcacgtttt
     6241 agtgaggcta cgttgattcg tgcactagaa gaaaacgggg tagggcgacc ttccacttat
     6301 gcaccaacac ttgatacgat ccaaagacgc tactatgtaa aattgacaca aaaaagattt
     6361 gagccgactg aactaggaga aattgtcaat tcattaatct gtgaattctt ccctcaaatc
     6421 gtcgatatcc atttcacagc agaaatggaa ggagatttgg ataaaattga agaaggcacg
     6481 gaagcgtggg tcaaagtggt tgatcgtttc tataaaccgt ttgaaaaaga actgacgaat
     6541 gcggaagaaa aaatcgaaaa aattcaaatc aaagatgaac cagcaggatt cgattgtgat
     6601 gtctgtggac atccgatggt gataaaatta gggaaatatg ggaagtttta tgcttgcagt
     6661 aattttcctg attgtcggaa tacgaaacca atcgtaaaag aaatcggtgt gacttgccca
     6721 gtatgtcatg aaggacaagt cattgaacga aaatctaaga aaaacagact attctatggt
     6781 tgttcccgtt tcccagaatg tgaattcact tcttgggata aaccagtggg cagaaattgt
     6841 ccaaaatgtg acggatatct ggtcgagaag aaagtcaaag gcggtaaaca agtagtctgt
     6901 gtaaatggag attacgaaga agacatccaa aaataataga aaagaggttc tttcattgaa
     6961 aatggtaaat gttgttggag cagggcttgc cggaagtgaa gctgcttggc agatcgcaca
     7021 agcaggagtc cctgtccgtt tatatgaaat gcgtccagtc aaatcgactg aggcgcatca
     7081 cacaagtaat tttgctgaac tagtatgttc caattcatta agaggaaaca gtttggctaa
     7141 tgcagtaggg ttattgaaag aagaaatgcg ccgattgaat tcggtagtaa tcacttcggc
     7201 agatgaaaca gcagtgccag ctggtggtgc cttagctgtt gaccgcgatt ctttttctga
     7261 aacaattacc cgaaaagtca aagagcatcc attagtaaca gtgataaacg aagaaattac
     7321 tgaaataccg gaaggaatca ccgtagtagc aacaggacct ttgacttctc atccattagc
     7381 agaaagcatc aaagcattca atggttcaga tggtttttat ttctatgatg cagctgcacc
     7441 aatcgtagac aaagctacga tcgatatgga taaagtatat ttgaagtccc gctatgacaa
     7501 aggagaagca gcttacttga actgtccgat gacagaagag gaattcaaag ctttccatga
     7561 ggcactaatc aatgccgaag tggcagaact taaatctttt gagaaagaaa aatattttga
     7621 aggatgtatg ccaattgaag taatggcaca gcgaggattc aaaacgatgc ttttcggacc
     7681 tatgaaacca gttggattgg aagatcctaa aactggcaaa cgtccttatg ctgttatcca
     7741 gctgcgtcaa gacaatgctg cagcttcttt atacaacatc gtgggctttc agactcactt
     7801 gaaatggggc gagcaaaaac gtgttttccg gatgattcct gggttagaaa atgctgaatt
     7861 cgttcgctat ggtgtgatgc accgaaacag ctttatgaat tcgccagaat tgctgaaacc
     7921 gacttaccaa tcaacgaaac gcgaagatct gttctttgca ggtcagatga ctggtgtaga
     7981 aggctatgtt gaaagtgcag cgagcggact acttgctgga atcaacgccg cacgcttagc
     8041 aaaagaaatg gaaccagtcg agtttccaca agaaacagct attggaagta tggcttacta
     8101 cattactcat gcggaaggaa aacatttcca accgatgaat gccaactatg gtctgtttcc
     8161 tgaactgcca gagcgcattc gagacaaaaa agcaagatat gaagcaattg cacaacgagc
     8221 tttaaaagct aatgaggcaa taaaaacaga attagaaaaa actgcaatcc caaatgagta
     8281 aggaaactcc atgaagaggg tgagacggat agtctcgctc ttttt
//
LOCUS       DAZUKR010000113         8314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000113 DAZUKR010000000
VERSION     DAZUKR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.115"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG89C_002234"
     CDS             476..1339
                     /locus_tag="ACG89C_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4900757.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG89C_002235"
     CDS             complement(1468..3090)
                     /locus_tag="ACG89C_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4900758.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG89C_002236"
     CDS             3339..3860
                     /locus_tag="ACG89C_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4900759.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG89C_002237"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG89C_002237"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS4900760.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG89C_002238"
     CDS             5941..6759
                     /locus_tag="ACG89C_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS4900761.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG89C_002239"
     CDS             complement(6862..7059)
                     /locus_tag="ACG89C_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900762.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG89C_002240"
     CDS             7228..7806
                     /locus_tag="ACG89C_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4900763.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG89C_002241"
     CDS             7901..8038
                     /locus_tag="ACG89C_002241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900764.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2503 a         1489 c         1640 g         2682 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gttt
//
LOCUS       DAZUKR010000114         8300 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000114 DAZUKR010000000
VERSION     DAZUKR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.116"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..526)
                     /locus_tag="ACG89C_002242"
     CDS             complement(29..526)
                     /locus_tag="ACG89C_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS4900765.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(656..1366)
                     /gene="budA"
                     /locus_tag="ACG89C_002243"
     CDS             complement(656..1366)
                     /gene="budA"
                     /locus_tag="ACG89C_002243"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS4900766.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1379..3043)
                     /gene="alsS"
                     /locus_tag="ACG89C_002244"
     CDS             complement(1379..3043)
                     /gene="alsS"
                     /locus_tag="ACG89C_002244"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS4900767.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3251..3913)
                     /locus_tag="ACG89C_002245"
     CDS             complement(3251..3913)
                     /locus_tag="ACG89C_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4900768.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3923..4738)
                     /locus_tag="ACG89C_002246"
     CDS             complement(3923..4738)
                     /locus_tag="ACG89C_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4900769.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4999..5442)
                     /locus_tag="ACG89C_002247"
     CDS             complement(4999..5442)
                     /locus_tag="ACG89C_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS4900770.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5576..5914)
                     /locus_tag="ACG89C_002248"
                     /pseudo
     CDS             complement(5576..5914)
                     /locus_tag="ACG89C_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5902..6279)
                     /locus_tag="ACG89C_002249"
     CDS             complement(5902..6279)
                     /locus_tag="ACG89C_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4900771.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6503..7696
                     /locus_tag="ACG89C_002250"
     CDS             6503..7696
                     /locus_tag="ACG89C_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4900772.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7862..8284)
                     /locus_tag="ACG89C_002251"
     CDS             complement(7862..8284)
                     /locus_tag="ACG89C_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900773.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2432 a         1673 c         1409 g         2786 t
ORIGIN      
        1 ctcttctttt ttgtttcaat agacgatttt agttattaat aaatgaaaaa gcatttatat
       61 taaaatttgc taataaaaga tctacatatt tttctagaaa tgcttgatcg atttcatcat
      121 accctttcgt tactccgcaa tcgatatcta aaacaccgat taaattacct tcttttacca
      181 ttggcactac gatttctgat tgtgcggctg aatcgcagga aatgtaattt gcatgggttt
      241 tgacatcatc aacgatcaat gtttcttgtt tttcagctga ttctccacaa actccttttc
      301 ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg tcctaaaatc aattcattac
      361 catcgaataa ataatatcct gcaaagacag tctcaggcaa agtctcattc aacaatgcag
      421 atgaatttgc taaatttgct aaccagttat cttctgcttc taataatcct tgttgctgta
      481 ataataaaat ttcgtatgca tcttttttct gtttttctgt ccacatgata aaaaccgctc
      541 ctttttgatc tatggagaag attatagctc agtttttttg taaacgctag gtttcctcct
      601 caaaaaaacg agatacgtca ggttaatcac cttgaacgca tctcgctgtc tttttctatt
      661 ctgcttgttg gatttctgct gctaaatcag cataatcaat cgtttcgtga acaaaagtcg
      721 gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg gctaatctca acttttcctt
      781 cagccacttc aaaatccatt acatgtccgc caaatgtatg tgtatcatcg ataaaatgca
      841 ggtgaaatcc cgctgcagca actccttgga ataattcagg cgtaaagaac ccgactaatg
      901 ttccttggat attgctttgt tggaattctg gttgagactc tgaaattttt gctagtcttc
      961 tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat atttttgaat aatccagtta
     1021 tcttcactgc caaaaatagg ttttccccgg cttctcttac taatatgtct tttttcaatt
     1081 gttctccatc tgcagttttt gagacagaaa aactagtatc tggcgtaaaa tctgtaattg
     1141 ctgcataggg ggtaagttca cttccatcta attgggcaac tgagccatct gatcttcctt
     1201 gataagctac gccatctaaa aagatgactt caccatcaag cccatgcaat gttcccaatc
     1261 ccagagttcc tttttctaat atatgagcaa tcgtttctgt tccatccatc aagccggcca
     1321 tcaaagctcc taatgtcccg tgctggtata agattttttc agtcatgtcg acacttcctt
     1381 aatagaattg atctggtaag attgtttctc ccaatttctc attatcacgg taatcaatcg
     1441 gaatatcaat aattacgggt ccttctgtcg caaatccttc ttctaaagct ttttctaatt
     1501 cctctacact tgtagctcgg attcctttag cgccaaaagc ttctgcatat ttcacaaaat
     1561 caactggacc aaagtctaca cctgacgccc gctgatattt cattttttct tggaactcta
     1621 ccatattgta gtgtccatca ttccaaatca aatgaacgat attcaatttt ttacgtaccg
     1681 ccgtttctaa atcctgggca gagaataaaa atcccccatc gccggaaact gatacgattt
     1741 gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt
     1801 gcataccatt actgaataac aaatgacgag gttcataact tctaaagtgg cgtgccatcc
     1861 aaatataatg actacctaca tctacggtaa cagtcatatc atctgttact tttgactgaa
     1921 gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc tccttttgat tgaacaatgt
     1981 cccgctccgt taattttgct tgaagagatg ctaaatattc ttttgcatct tctgacactt
     2041 gttgaggctc taatgaacct gtcaataaat caagagtggc tgagatatca ccgatcaatt
     2101 ctctttcagg ctgcataaaa ggatcgattt cagctggcgc ttcatcgatc acaatgatcc
     2161 gcgcatcttt ttccgcattc cagtttcttg cttcatattc gattggatca tagccaattg
     2221 caataactag atcacttcgt ttcaaaagca tatctcctgg ttggtttcta aataaaccca
     2281 cacgaccaaa gaaatgcgct tctaattcac gtgagatcac tccagctgct tggaatgtct
     2341 ctacaacagg caatgctgtc ttttcaacta attgcctgat ggctaacgtt tctttttcac
     2401 ttgatcctcg catacctact aatagtaccg gtagttttgc ttctctaatg cgttcagcta
     2461 ggtaacggat atcatctgca gaagcagaac ctaatttagg atcgctcaat ggcttgatga
     2521 cattaccttg gacaggtgca tccactacat cttgcgggat actgataaag ctcgcgcctt
     2581 tttttgaact cttagccata cgataagcat tggcaatcac ttctgaaatt gtttctggat
     2641 cttggatttc tgcactgtat tttgtgattg gctggaacag tgctgcatta tccatgcttt
     2701 gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt
     2761 caccttctgc tgtcgctgtt accaaacctg tagctaagtt actagcacca ggaccacttg
     2821 ttgcgataac tactcctggc tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag
     2881 cattttgttc gtgacgagtc acgattaatt caggaccttg atcctctaat tcattaaata
     2941 caccatcgat ttttgcacct ggtataccga atacataatc aacatgatga ttaatcaaac
     3001 tttctactac agatttcgaa ccttgtttca ttttttcagc cattgaacat cgtccttttt
     3061 atgctttatt tttaattatt agttagaaat aatcacaaat aagtgataca gtttctaaac
     3121 ctgtttcata caaaagtata gcacgtttta tttcatttgt ttagccatat gtttcaaaaa
     3181 atgaaaaaga atgacagttt ttacataaaa aaaactgtca ttcttaattg aaagtttcaa
     3241 tttataaagt ctacagatcc atagttctca tcaatttacg attgttgaag taaatcaaac
     3301 caaatacgat agaaggacct agtagaatac accattgggg ctttacgaaa aagaagagaa
     3361 acaaacttgt caatagtaaa aaatctagta aactcacata ccattttttc aacgtatagt
     3421 agaacgctat ccgtagaact tgatttggtt tcgacgctgg atttctcacc tgaaaatacc
     3481 agcagttggt gctaaaggaa actgataaac aagcaagtaa aacaaacaac ggaatcaacc
     3541 attttccata agaaaaacgt ataaaaaaga aacaatcaaa aatcattatc acactaacta
     3601 tccaagcaaa tagccaataa aaaaatccct ttagccaaaa ttggcgaaac atctttatga
     3661 acaaacgtac tggtgaaaaa ctattttctt ttgctaattc ttctgtcata cctatcatca
     3721 cgatgcaacc tggaccaata aaaagtaaag aaaaaataaa ggaaaagaca ttccttccat
     3781 ctatcgacaa aaggaacgca gctgctatat acaagatatt gacacaccaa aaacaaaggc
     3841 tacttgccaa tgattgatac aaccaataaa agaattccat ataaacattt ttgtcaaaac
     3901 gctgctcttt cataatcacc tcctattcct tcattcctga tgaagcgatc ccttctacaa
     3961 aatatttttg catcgctaaa aatataaaag caacaggaag aacagaaaca actgaggcag
     4021 ccatgattaa tgcgtactct gcatcaaata gcccaataaa cattttcaac cctaactgga
     4081 ttgttttatt ttccgtagaa gacaaataaa tgaatggccc catataatcg ttccatgtat
     4141 taacgaatgt gatgatcgtc aaactggcaa tcgccggttt tgtaagaggt aaaatgatct
     4201 tgcgatagat tccccactct gataagccat cgatccgcgc actctcacat aaagaatctg
     4261 ggatactact gtaatattgc ttcaacaaga acacaccaaa agcattgaac gcttgtaata
     4321 gcaccaacga ccacaacgta tctgttaaac ccacttttct catcataatg aactgcggga
     4381 tcatataaga ctgccaaggg acagcaatcg tgccgatata tgctaaaaat aaaatatcgc
     4441 gccctttaaa atttagttta gaaaacccgt aagcagcaaa actagaagta aacaactgaa
     4501 tcaacgtgat cacaacgctt aaaaaagctg tattcttaaa aaatgtcact aaaggaatac
     4561 gagaccaaat aaccagataa ttctctaaat gccaagtttt agggatccat tgaatcggaa
     4621 tcgtgaacac ttcattattt gctttgaatg aagcagaaac catccataaa aaaggaatga
     4681 tcgtcaataa agctaataca atcagcagaa tcatacccaa ccattgaata aagggcatgt
     4741 tttttgaacg aatttttgct ttttttcttt catttcgaac acctccaaag ttataatctt
     4801 catttctttg taatgattac aatccagaac aacggttatt atagctgatt catttccgtt
     4861 cttcttattt ttgtaagcgc ttatttaaca agttatgtaa atctctttat ttttttgatt
     4921 taaaaatgaa aaaatacaaa agcaagactg tcatacgtct cgcttttgta ttgaaatttc
     4981 ttcgattaga gatacttatt actgttcaaa agcttctttg aaacggtgaa tcaattcatt
     5041 gactacaggt gataaaatcc gattcttttt ccagactagc atgcagttcg tttcaatcat
     5101 tgggtcaagc ggaacgaatg tcactcgctt tgaatgatga cttacagcac cttctatgac
     5161 taaagcagca cctacctgat tctcaattag cgcaaataca ttgaaaatca aattgtacgt
     5221 tcctgtgatc agcaactgct ccttttttat gcctaaccag tcagcaaaca ttcgctgtat
     5281 ttctggacgc tttgagatca aaagaggtac attcgcaaag tcttcttttg ttatcacgtc
     5341 tttttttgct aaaacagaat tttctaaaat gagcaatccc catttttcct ttcttggtaa
     5401 caaaatcgtt tcgtatttct ctgtatctac tggatctagc aaaatcgcta aatctaacag
     5461 gcttttttct agtcactccg tgatatcatc acttgttccg ctgtggatat gaaattgaac
     5521 gctgggataa tcacggatca acttttctag taatagtgcc aatgtattcg aactatcagc
     5581 ttccacacag ccgatcgtaa atcggccgct gaataattgt ttttttctgt tctcgaattc
     5641 ttgagtggtt tgattcgtta aatctaaaat ttcttctgct cggctttttt agaaaataac
     5701 ctgcttctgt caagatcatt ttgcggtttt cgcgtacaaa cagttctgtt cctagttact
     5761 cttctaactc cttcagttgt cggcttaagg ttggttgtgt aagatgtaga acttctgcgg
     5821 cttgagagat tgttccttct tctgcaattg tccaaaaata ttttagtaag cgtaactcca
     5881 tagcgatgct ccttgatttt tttacttgat acattccatt ttatttctag ataacacacg
     5941 atcatatttc ttgatttttc cttgtaaacg ttccaaacat tcttgcattt cagcaatttt
     6001 tttcgtcaaa agttcacttt cttcaatcaa aagcttacgc cgctcaaata cagttgcttc
     6061 tccttcgcta gataggcgaa tatattcaat caaagactcg atcgataatc cggcatttcg
     6121 catacagatc gtaaagtcga cccattttag gtcttcttct gtataatcac ggattccgcc
     6181 ttttgtccga ttaactggtg gaatcaaccc gattcgttca taatagcgca atgtatcggc
     6241 cgaaatgccc ttttcttcac tgacttgttt gatattcatc tgatcactct ttctcttaat
     6301 ttcttgccac tttacttatg tattttattt attctacaac tttaagtgta ctccaagtca
     6361 atactcattt ctattaaaac taagctggat tatctatatc ggttgtttgt gtattatttc
     6421 aaataaaaaa gtcttgtttt tttattcttt tttggatata cttttaacaa tttagggaaa
     6481 ttttaggaaa aggggtataa aaatgacaac gtatcagcga aaggtattag tatcgacatc
     6541 ggcgggaatt gctttagaga atatggatat tatgttttta gctttttcat tatcttcaat
     6601 gattacttct tttcatatta gtggtacgca agcgggtttc atttcaacga tcactaattt
     6661 ggggatgctc attggaggta ttttttttgg catcatagct gataaatatg gacgtgtgaa
     6721 agtattttct caaacggtcc ttctgttttc gattgcttct ttattgatgt attttgcttc
     6781 aaacatctac ttggtttatc tctttcgctt tatcgcaggt gtaggagcgg gaggagaata
     6841 tggggcgtgt atgtccttga tttccgaaag tttttcaaaa aaacaaattg gacgagcttc
     6901 ttcggttgct ggtattggtg cgcaggtcgg tgcggctttg gctgcgattt tagctgctgt
     6961 gatcattcca acgtttggtt ggaagatgtt gtatgtcgta ggtgtactgc cggtgttgat
     7021 ggtcttgttc attcgtcgcg gcttgactga accaaaggaa ttcaaatctg tacaatctgc
     7081 cgaaaagaaa accaaactta gcagactgtt tgaatcaaaa catcttgctt ggcaaacatg
     7141 tggccttagt atcatggtaa ctgtccaaat tgccggctat tttgggttaa tgaattggct
     7201 accttcaatt atgcaaaatc gattagggct ttcggtttct gggtcttcat tgtggatggt
     7261 cagtactatt ttaggcatgt ctttggggat gatgactttt ggcataatca tggataaatt
     7321 ggggcctaaa ctagcctttt ctttgttttt aatttgttca tccttatctg tctatctact
     7381 tgtgttagcc catagccagt ggagtttggt agcagccgct gtgatagtcg gctactttat
     7441 caatggcatg tatggtggat atggcgcagt gattagtagt ttgtatccaa cagaaatccg
     7501 cgcgacggcg aataatttcg ttatgaacct tggacgagct gttggtggtt tttcttctat
     7561 cgtcatcggt ttcttgatgg atcattatag tttaactgcc gtaatcttgt ttttgagcgg
     7621 tatttaccta gtcagtctgc tggtcttgac aactcttact ggtgttacag cattaaggga
     7681 aagcttgcag cattgatagt tgagtctgta aatgacaaga aagaggtgtg gaaaaagctt
     7741 gcatctgcgg taataagacc agccaaatca aaaacatgag acactgtttc cgtttggctg
     7801 gtcgtattac ttgatgcagg gcagcttttt caactgctaa tttccttcta tctggcggcg
     7861 actattcttt gacgtccgtc atttcaaagt actttactaa taacttagca taacgaaaaa
     7921 ctgtggatac actcgtatat tgagcgtctg cataatcttc caaatttttc tcaggatgtt
     7981 gatacatgaa ttcaatcaaa tgataattat atgattttct gtagaaatga agataaataa
     8041 tgtagattgg gaagttctcc tgcttttcca tagaaaagcg ccgattattc tcattcttca
     8101 aaatgacctt atcaggatat tgttggaaca attcttctaa atctgttaga taattcaaga
     8161 tagacttact tgataccttc aattcgtttt gtagttcttc tattgaacac caacgttctg
     8221 tttcaagcag taaatgaatc attttaattt tcttttgaga aacttttgtt aaaaaagtct
     8281 gcatattttc tcctcctgta
//
LOCUS       DAZUKR010000115         8288 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000115 DAZUKR010000000
VERSION     DAZUKR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.117"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..948)
                     /locus_tag="ACG89C_002252"
     CDS             complement(<1..948)
                     /locus_tag="ACG89C_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4900774.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEEL"
     gene            1180..2013
                     /locus_tag="ACG89C_002253"
     CDS             1180..2013
                     /locus_tag="ACG89C_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4900775.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2047..2886
                     /locus_tag="ACG89C_002254"
     CDS             2047..2886
                     /locus_tag="ACG89C_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4900776.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            2912..3472
                     /locus_tag="ACG89C_002255"
     CDS             2912..3472
                     /locus_tag="ACG89C_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4900777.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3671..5392
                     /locus_tag="ACG89C_002256"
     CDS             3671..5392
                     /locus_tag="ACG89C_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS4900778.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5557..6699
                     /locus_tag="ACG89C_002257"
     CDS             5557..6699
                     /locus_tag="ACG89C_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4900779.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6715..7926
                     /gene="thiI"
                     /locus_tag="ACG89C_002258"
     CDS             6715..7926
                     /gene="thiI"
                     /locus_tag="ACG89C_002258"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4900780.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2849 a         1440 c         1725 g         2274 t
ORIGIN      
        1 caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg
       61 actattaata ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc
      121 ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat
      181 tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc
      241 taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc
      301 ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag
      361 tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat
      421 gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta
      481 gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc
      541 acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt
      601 acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag
      661 aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc
      721 tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg
      781 tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat
      841 ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc
      901 tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc
      961 cttacgaata attttgcata gccgttaaat cttattgtat actatataaa agagttttgc
     1021 tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg
     1081 gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa
     1141 gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca
     1201 tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca
     1261 gggtttctac ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca
     1321 gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa
     1381 ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg
     1441 cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc
     1501 caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga
     1561 aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat
     1621 gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc
     1681 gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac
     1741 tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag
     1801 tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat
     1861 gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct
     1921 ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat
     1981 ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg
     2041 aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca
     2101 aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc
     2161 ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta
     2221 gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac
     2281 cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga
     2341 caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa
     2401 tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg
     2461 aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag
     2521 atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag
     2581 ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc
     2641 atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt
     2701 gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt
     2761 gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt
     2821 gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt
     2881 tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt
     2941 agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga
     3001 aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc
     3061 tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga
     3121 taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca
     3181 agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat
     3241 agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga
     3301 tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat
     3361 ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat
     3421 tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt
     3481 tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga
     3541 atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta
     3601 tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga
     3661 ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc
     3721 cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc
     3781 cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt
     3841 taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg
     3901 gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa
     3961 cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac
     4021 aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga
     4081 aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa
     4141 agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca
     4201 gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc
     4261 tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct
     4321 gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga
     4381 agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga
     4441 tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga
     4501 aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc
     4561 gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc
     4621 aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct
     4681 ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt
     4741 ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca
     4801 acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga
     4861 agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga
     4921 aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt
     4981 tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc
     5041 tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga
     5101 ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca
     5161 tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg
     5221 tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga
     5281 atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg
     5341 tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa
     5401 agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata
     5461 tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt
     5521 ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca
     5581 acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt
     5641 gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca
     5701 agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc
     5761 ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg
     5821 aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta
     5881 aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc
     5941 gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat
     6001 aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct
     6061 aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg
     6121 ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga
     6181 actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg
     6241 caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa
     6301 gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa
     6361 gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact
     6421 gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc
     6481 catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa
     6541 catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct
     6601 gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt
     6661 ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa
     6721 tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc
     6781 tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa
     6841 atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt
     6901 attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa
     6961 aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg
     7021 aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat
     7081 gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa
     7141 atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg
     7201 tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt
     7261 atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg
     7321 gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa
     7381 gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg
     7441 ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca
     7501 attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta
     7561 gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg
     7621 gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt
     7681 gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa
     7741 gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc
     7801 catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg
     7861 aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg
     7921 ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa
     7981 tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat
     8041 ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt
     8101 tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga
     8161 ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc
     8221 aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc
     8281 agaggttg
//
LOCUS       DAZUKR010000116         8153 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000116 DAZUKR010000000
VERSION     DAZUKR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.118"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(471..2102)
                     /locus_tag="ACG89C_002259"
     CDS             complement(471..2102)
                     /locus_tag="ACG89C_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS4900781.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2116..3789)
                     /locus_tag="ACG89C_002260"
     CDS             complement(2116..3789)
                     /locus_tag="ACG89C_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4900782.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(3802..4119)
                     /locus_tag="ACG89C_002261"
     CDS             complement(3802..4119)
                     /locus_tag="ACG89C_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4900783.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4144..5544)
                     /gene="lacG"
                     /locus_tag="ACG89C_002262"
     CDS             complement(4144..5544)
                     /gene="lacG"
                     /locus_tag="ACG89C_002262"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4900784.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5781..6566
                     /locus_tag="ACG89C_002263"
     CDS             5781..6566
                     /locus_tag="ACG89C_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4900785.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6569..7261
                     /locus_tag="ACG89C_002264"
     CDS             6569..7261
                     /locus_tag="ACG89C_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4900786.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7535..7906)
                     /locus_tag="ACG89C_002265"
     CDS             complement(7535..7906)
                     /locus_tag="ACG89C_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS4900787.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2504 a         1799 c         1396 g         2454 t
ORIGIN      
        1 ggggaatccc tataaatcaa cattcttcaa tagctaaaat gcttgatttt cattaaaaat
       61 aagatggttc cctaccaaaa gagactataa tataagttaa tttgtttcat tcttatcata
      121 aaaagaagtt ctcgaaaaac gaactgtttt ccgagaactt ttttctatcc atctaagtgc
      181 tcaaatcata ttcacgacgg aattctaagg ttaccctttc ttaaacaaac tttatgttat
      241 ctgcacaact ttttgcaatt ttttcattgc ttcttacgaa atcgttgaac tctctttagc
      301 ataatacaaa gtgtagatga agtctggtgc tttactcgtt ttggtaaaaa tgactggaaa
      361 tcgtttctgg ttatttttgg caaaggcgag gtgcaggttc gattcctgcc ttctacgcta
      421 acgatgtggc tgaagggaac ctccgatctt gaatcgaaac gatccgcagt ctattgtttc
      481 accaatgaaa caaaatcaac gttcgtccaa gcatttcctg aagccttgct ataaacccca
      541 atttcacaaa cacctgttgt aattggaatg tctttgattg taatttttgt ccattcactt
      601 gttttaacaa ccgcagtatt tagttccgct ccaccataat ttttgatata taactgatta
      661 gtatcaaact ctcccccaga cattacatag gcactcaatg tgtaagttcc tttgcttatg
      721 ccactaacat tttgataagc tgatgcctcg tatgcttgat cctgccaaaa agttagtttg
      781 tagtccccaa aaaaagtgcc cgcttccgtt ttgatcgtac ccttagcggt tccttgttga
      841 aaccaggttt gccaattgga aggactggtc gttggtccat cgctttcgaa acttgcattt
      901 ttaactagat ttatttgcgc ccctgcttgt ttaaacgcac gcaagcttgg caaggcgttt
      961 ccagcaaaat caaacatcgc ttggttgtcc cattcgtttc ccatagtgga ttgaacactg
     1021 agataattca tacctgcttg tgttgcccaa ctcacatttc cgttgtacca aagaggttcc
     1081 caatagaaga agcccgcccc gcgattattc ggtactgcct gaatccgatc ggcaagatcc
     1141 agcaaatact ctgcttgtcc tgtcggtgtt ggttcatatc cccccgcagc tgcttcggct
     1201 tcaccaaaag catttggcat agtatcggca ttcgctgttg tatgcccata agctgtttca
     1261 actacgatca cttctttacc ataccgttgg ctgatcgcat tcatattggt ttgaagttca
     1321 ttcattgaac cgtgccaata cggataatac gacactccaa ttaaatcaaa tgacacacct
     1381 cgactcgtga tttcatcaaa ccaccaagta aatgctcctg tatcgccacc atcagctaag
     1441 tggagcatta ctggagttgt tttaggttga gtgtctcgaa cagcttggat accagacttc
     1501 aaaaaagcag ccaatcgatc aaattctccc ccgccttctc cccaagactt gccataaggc
     1561 cagagcattc cgctattcag ttcattgccg atctgaacca tatcaggata aacgtccagc
     1621 tgcttcattt ccgacagaac gttctttgta tagtcgtaca atgccgtatt catttcttca
     1681 aaggtcagac cttgccatgc tttaggaaga ttctgtttcc caggatcaac ccaaaaatca
     1741 ctgtagtgaa aatccagcag caccttaagt cccagatttt ttgctcgttt tgcaaggtca
     1801 atcgtcgttt ccagatcatt tgtccctgct ccataacttt ctccattact attataagga
     1861 tcctgccaca aacgcagtcg aacatagttg acaccattat tttttaaaat ctctaaggca
     1921 tcttttgaaa tatcgtcttc ataatatttt gcaccagatt tttccatatc tgacaggatc
     1981 gaaatatctg ctccgcgaat aaattcgctg gcagatgctt caacagtttt tgaaacagcc
     2041 cccaaagccc ctaacacagc tgcaaataaa acagctttac gcaaccaaat tcccattttc
     2101 attatggctc ctcctttatt gaaattggtt cagaataaac tgaacagctt tttcaggatc
     2161 tctcgttaat ccgatatatt ctgctccttt tgtcgcaacc agcttgattc ctaagcgatc
     2221 ggtgtcttct ttgatatcat catagtaaga attgacttgc ggtgctaaga ccaccatatc
     2281 aaaatcctgc agcatttcgt aatgagaacc ataagcaccc gccattgcac tgatcggtgc
     2341 tttcgattca gatgcacctt tttttaaagc attagctaac atcgcacttg tccctgcgcc
     2401 agcacaaagt accaacacat tgatttcttt atttgcatcg ctgataactt gactcgctgc
     2461 cacaggtact tcctcacgta catcggcaga agcaccttcc tcggttgcga tcacctctgc
     2521 tttttgtgct tctttctcta ctaagacagc atcgtatgct ttgcaaaatg gtagatagat
     2581 caggaagtca acgacgatta gaaccgctgc tagaacaaaa gctaagatac ccatgcccgt
     2641 tcctagaacc aaaccgatcg gtgctggagt tgcccaaggc aaaacgtaca taaagctgtt
     2701 catgcccaat acatcgacaa aaaacttgaa caggcaaacg tttacaacag gtgttaacaa
     2761 aaatggaata aaaaaataag ggttcaaaat catgggtgcc gcaaatagca atggttcgtt
     2821 gacagcgaaa cttactggaa taaaggaagc tttcccaact gccttcaact gcttagactt
     2881 agcaaagagt aggaagataa aaggaacaac caatgttgcc ccagttcctc ccatagttcc
     2941 tacaaaattc cctaaaccca ccgtcagtac tttagaagct tgttcaccgg aattgaacaa
     3001 ttgcagattg gtttccacat tggcatagat aattgccgca attgccggct ccacgatcga
     3061 cggtccatga acaccgacaa accaaaacag cgccatcgct ccccagataa tagcgattcc
     3121 taagtacccg tcagctgctg taaatagcgg ctgcagcacc gtaatgattg cttctgcaaa
     3181 tgagatttgg aagatttgtc gaatgcctaa atcaattaaa acggccgcaa atactgaaaa
     3241 agagaaagga aaaatatctc ggaaggtttg cgaaatcgtt ccaggaactt ctttaggcat
     3301 tttaatggta atatctttca gcacacaaaa cttatataaa ttaactgtta aaaatgcagc
     3361 gacaaaggaa gccagtagtc cctttgtccc catatattcc gttgaaaatc caccttcaat
     3421 cggagagacg cttaacaaca ggaaacttac cacagaggcc agcatcacag aggtggtgtt
     3481 gattactttg tttttcggta agcgacgatt catcgaatca gccaagcagc gagcagtggt
     3541 tgctgaaacg agtaacccta caacacccat cgaataatta tagactttcc atagccatgt
     3601 ggagacagat tcaggccatt ggtaaccaaa aatctcagga accgcagcaa tcaaaatgaa
     3661 aatgcttgaa aataaaatag ctggcatcgc tgttaaaaat ccgtcacgaa cggctcctaa
     3721 ataactatta cgtgagattt tttcaaaaaa tggtttccct ttttcgattt gtttaatgat
     3781 tccatccata gcgttcttct cctatccttg attctgtctg tacagtacaa taaagtcttg
     3841 aatcagatct ctcagcaata aagtggtcat taaatgatcc tgcccatgga taaaaatgaa
     3901 tcccatctcc atctctttcc cagatgcttc ttccgctaaa atttttgttt gcgaattatg
     3961 tgcaagggtt aaattttcgt cggcttcttt caacgccgaa tcaacatgtt caaagtttcc
     4021 ttgtcttact tctttcaata atttcaataa tgtactgcga gcgtcgcctg aatatgcaac
     4081 gatctcaaaa ccaagcattt gtaattcctc tttattcatc ttttcttcct ccattatcac
     4141 ttattaaata atcgttttgg tttcactgac caacttgtac cagtaagctg attcttttgg
     4201 gtaacgcttc tgggtttcaa aatcaacata aaacaagcca taccttttgt tatacccatt
     4261 tgtccatgag aaaagatcca taagcgacca caaaaaatac cccttcacat ttaccccggc
     4321 tgttatcgcc ttgcttaagg attccaaata gactctcaaa tagtcgattc tcggctgatc
     4381 cataataatg ccatcttcaa actgatcttt ataccccatc ccgttctcag tgatatagat
     4441 tttgttgtaa tgtgggtaat cgcttttaat tcgcagcaac aagtcatata gaccttctgg
     4501 ataaatcagc cagtcccaat cagttcgagg aattccttct ttataaatac gttctccgat
     4561 tcccttcact ttataaacag aggtcccttt ttcacccgta ccgttatgat ggattgcatt
     4621 ttctccatcg taagctttga caaaatggca ttggtagtga ttgatcccta agtagtcatt
     4681 acgcgtggat gcttttttca attcaacgaa atcttcttca ggaaaatgat agctggcctg
     4741 atttgcttca catatctcat caagagcagt caaggtttca gttgaataat agcctaaata
     4801 ggtagcatct aataagaaac ggatcgacaa tgcatcgtct aaaaaggcag catgcttatc
     4861 ttccggcgcg tctgttgccg catatttcgt ctctaatgag tgaactacac caatttcacc
     4921 cggaagttcg ttttctttga aatagttcac tacacgggca tgagcaacca tcatattatg
     4981 aagacaggcg acgatctttg taaaatcata tttgattcct ggtgggaaaa cacctaacaa
     5041 atattgattg gttgcaactg gatagatttc attaaaggta ctccacacct tgacttcttt
     5101 gaattcatga aaacaaaaaa tggcatagga aacaaatgct tctattgttt ctcgattcaa
     5161 aaaatctcca tggtcaaaca aacgtttagg ggtatcaaaa tgatgcaacg tcacaaacgg
     5221 cgttacgttt cttttcttac attcttcgaa cacacgatga taaaaagcga ttccttctgg
     5281 atttggtttc ccagctccat caggaaaaat gcggctccaa gcaatggata atcttagacc
     5341 atttacacca aaacgttcac acaattcaat gtcctttgca tattgatgat aaaaatcact
     5401 tgccggatcg ggactgaatc gcccttgttc ctctaaaaag tcatcccaag caacagcgcc
     5461 tttgccacct tccttagtgg ctccttcaac ctgataggct gctgtggctc cgccaaaaat
     5521 aaaatcttct ggtaatttaa gcatgtacat ctctcctcaa atctataatc agttttcgct
     5581 ctttctaact gattgcattg taaacgcatc caaaacattt gtcaacaaaa acaaacacaa
     5641 ataatcataa aaaccagaaa acagcaataa aatctattgt aacaaacata aaaaccaccc
     5701 taaatgttta cttttgttcg aattagattg actgttgtcc gtttatatga tagattatcc
     5761 ttaaattaga ggtgaagaca atgttgaaaa aagaaagatt gcttactatc gtagaaatgg
     5821 tcaataaaaa aggaatcctt accgttaatg aaatcatcaa tcaattagat gtttctgata
     5881 tgaccgttcg cagagattta gatgaattag aaaaatcggg caagctgctg cgtgtccatg
     5941 gaggggctca aagtctttcc tacactttag atcaagaact ttctcacact gaaaaagcca
     6001 ctctgcagat tcaagaaaaa aaagaaattg ctgccacagc agcctccttg attcacgagg
     6061 gagaaacgat tttcttagga ccaggtacaa caatcgaact attggcgacg catttactga
     6121 acaagaaaat tcggatcatt acaaataatt atccagtttt tgatattctt gctcaagcag
     6181 atggaccaga aattctactg attggcggtg atttccggaa aaataccggc gcatttgtcg
     6241 gaccgatcac caacgacaat ttaagaagac taaagtttac aaaagcattt atcagttcaa
     6301 atggcgttca caacgaagcg atctctactt acagtaccga agaaggagaa gcacagcaga
     6361 tcgctctaaa taattcccgt aagaaatatc tactcgttga taataaaaaa ttcaatcgag
     6421 atgattttta tgtgttttat aatttgcatg atttcgatct gctcattacg gatcgccaga
     6481 tctcgcggga ggttcaagag cactatgcac aatatgtcga tctgcaaatt gctgattcaa
     6541 ccgaaaatac cactatagga gcgtaactat gaaagggatt atatttgatt tcaacggaac
     6601 aatgtttttg gactctcatc tgcatgaagc tgcttggctg catatgatac atgatcatac
     6661 aaaagatggt ttatcagacg aggatatttt aaaaaatatt catggtcgga cgaatacgga
     6721 gattttgaaa catttcattt cagagcattt gaccgcctct gagatcgccg tattgtcaga
     6781 agaaaaagaa agctattatc gtatgctttg cttgcaaaat gaaaacgagc ttcagttgac
     6841 aaaagggctt gaagcgactc ttgaccagct gactgaccaa cagattccat taacgattgc
     6901 cacggctact gtcaaagaga atgttgcttt ttattttgat attttcgatt tagcccgctg
     6961 gtttgactgg gacaagattg tctatgacga cggctctttc cctgggaaac cgcaaccgga
     7021 tatttttctt aaagcggcag acaaactgag cttagctccg gctgattgtc ttgttatcga
     7081 agatgcttac tcaggattac ttgctgccaa acgtgcgggg atcggaacaa ttattgcaat
     7141 tgatccattc ggtaaaaaca aaacagtatt tgaacaggca cagctaaata aagatggaac
     7201 catcaaagat tttactggtt tttggacaga gcacctgtct gccacatccg ttaattctta
     7261 aacgattcaa ctaactacca aagaaaagca actgaaatgc acacctcctt ttataaaaaa
     7321 ctgccagtga aacgacatga aagatacact gctatatcct tagtgaaata tagcagtgta
     7381 tctttgactt tttcattctt ttaaccatat gacgcgttca tctctatcta taattaacat
     7441 taaaaatagt gttttcacat tatttctcaa accaatctta aaaatcatca ggagtacttt
     7501 tttgatatac ttttattaat gctgggcgta atggttaaaa gatttccact agttcctctc
     7561 caccaagctg agttgaaaaa tgcttatctg taaacaacat agacaaagag gaaataaacg
     7621 tttccgtatc tttctctgtc ccaaggatat taattaattt tcctactagg taagtatcat
     7681 taaacttaag atctgccttc acatcaaaag ttttgataaa agcttttatc atgcgtacaa
     7741 ttttgtcttt atcgccataa ttgatttcac cactcatatc aatatagtca ctaatccctg
     7801 aagaaataat ggcaactgct tcttctttcg ccttatcaga taattcattt aatgactggg
     7861 attgattgat gacttcttga gttttatctg ttacgtaatc tctcattatc ctatcggcat
     7921 actccgcaaa gtaatcttga aaaaattctt ctacagggta accaacttcg ttttcccaac
     7981 caggagaaat ttcattatca gtacttatta aatctattaa aaaccaaccc gttctctgag
     8041 tgttgattct ataatatttt taatttccta tataaatcaa aaatattata gaatcaataa
     8101 gctaattaag aaacaaactt tagaaactct agaagaactt ctgaatttat ata
//
LOCUS       DAZUKR010000117         7995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000117 DAZUKR010000000
VERSION     DAZUKR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.119"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            387..575
                     /locus_tag="ACG89C_002266"
     CDS             387..575
                     /locus_tag="ACG89C_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4900788.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="ACG89C_002267"
     CDS             653..1666
                     /locus_tag="ACG89C_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4900789.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1733..2767)
                     /locus_tag="ACG89C_002268"
     CDS             complement(1733..2767)
                     /locus_tag="ACG89C_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4900790.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2882..5107
                     /locus_tag="ACG89C_002269"
     CDS             2882..5107
                     /locus_tag="ACG89C_002269"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4900791.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5125..7008
                     /locus_tag="ACG89C_002270"
     CDS             5125..7008
                     /locus_tag="ACG89C_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS4900792.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6989..7963
                     /locus_tag="ACG89C_002271"
     CDS             6989..7963
                     /locus_tag="ACG89C_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS4900793.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2577 a         1458 c         1696 g         2264 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgggg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1741 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1801 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1861 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1921 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1981 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     2041 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     2101 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     2161 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2221 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2281 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2341 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2401 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2461 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2521 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2581 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2641 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2701 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2761 caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tattgacaaa attgtttagt
     2821 aaacagtata caggaattat aaaaaaaagt aaacggtttc cgttttgttg ggagtgaaat
     2881 gatggatatt acgcatagaa aaacagataa aaatttcatc ttatttgatg aaaagagccg
     2941 agtcttccat ttacgaaata catttctttc ttatctgata aagatagaag aaagcaatgt
     3001 attggcgcat atttattttg gcaaaccagt caagcagtac aaagacaata aaaattatcc
     3061 ccgaagagat cgtggatttt caggaaatgt tcccttaaat cctgaccggt ctctgtcgaa
     3121 agatacacta ccgcaagaat actcttctca tggcagcatg gactttcgaa cgcccgcatc
     3181 gatcattcag cgcaagaatg gatcagactt actggatctg agatatgaca gccattatat
     3241 tacagatggg aagccagaga tcgaaggctt gcctcaaact tatgtgatgg acaagtcaga
     3301 ggcacaaacg ttggtgattt cacttaagga tagagaaaca gcgatttatt tcgatttgtt
     3361 ctataccata tttactgata gagcagtgat cacacgatct gtaaagataa gaaatgaaac
     3421 cggagaaaca atcaaactag aaaaagcggc tagtttccag ctagactttg cccatactag
     3481 gcgatttgat gaagtcattg ctttgccagg agcacatgtc aatgaaagac agatttcacg
     3541 gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt cgaggaacga gcagtcacca
     3601 tatgaataac tttattgctt tagttcatca tcatacgaca gaaaatactg gagaagcgat
     3661 cggacttcaa tttgtttatt ccggcaacca ttctttcgaa cttgaaaaag atcagatcaa
     3721 tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt tcgtgggaat tgaatgcagg
     3781 ccagtctttt caaacacctg aaatgatcct ttcttactca agtcagggat tgaacaaaat
     3841 gagtcagatc caccatgagt tgctacgtga aagaatcgca agaggtcgtc atcaatttgc
     3901 tgaacggccg attctggtca ataactggga agccacttac tttgacttca atagtgaaaa
     3961 aatcaaggcg atcattgatg aagcaaagga gctaggcatc gaaatgttcg tattagatga
     4021 tggctggttt ggaaaaagag atgcggacaa ttcttcatta ggagactggt tcgagtatga
     4081 aggcaagttg acaaatggat tacgtgaaat agcagattat gctcatagca aaggattgaa
     4141 atttggttta tggtttgaac ctgaaatgat ctcggttgat tctgaactgt atcgaacaca
     4201 tcctgatttt cttatgcagg agccaggtag aatgccttct gcttccagat cacaacatgt
     4261 actggatttt acaaggttag atgttcggca gacgattgag aaacaaatga gaaagatttt
     4321 agatacgatt cctcttgact acatcaaatg ggatatgaac cgttcgcttt cagatgtcta
     4381 ttcgattacc cttgacccac agagacaagg agaagtagcg catcgttata tgctcgggct
     4441 ttatgaacta cttgaacatt tgacaactga ttatccggaa atcttatggg aaggctgttc
     4501 tggtggaggt ggacggtttg atgccgggtt tatttattat atgccgcagt catggacaag
     4561 tgataacacg gatgcagtgg agcgtatgaa aatccaatat ggaacaagcc ttgcttatcc
     4621 gatttcgtcc atcacagctc atgtatcagc tgtaccaaat catcagaccg acagatctac
     4681 ttcgttgaaa acaagaggag aaacagctat gagcgctgtt tttggttatg aactggattt
     4741 gacaaagctg tctcctgaag aaaaaaagca agtaaaagac caaattatat cctatcaaac
     4801 gattcgtccg gtgatccagt atggccatta ttatcgtttg gcgagtcctt ttgaagaaaa
     4861 tattgccgca tggatgtttg tgtctcctaa acaagatgaa gctattgtgt ttttagggag
     4921 gatactagct tcagctcaac cagcgttcca tgaagtatat ctgatggggt tagatgatga
     4981 ggcactttat caggaacaga cctcgaagcg gatattttcg ggggccgaat tgatgacagt
     5041 tggactttac ttccccgatt ttcaaggtga tttccaaaca gaactgcttc atttcaaaaa
     5101 gttatgagag agaaggaaaa aagtatgaaa gcaaatataa aatggttaga tgatccacaa
     5161 gtttttagag tgaaccaatc aaatgcacat agcgatcatg tttgttatgc aacaagggaa
     5221 gaagtagaaa ccacatcctc ccttaaacag tctttaaatg gatcatggcg tttttgttat
     5281 tcgccaaatc cttcggtacg accaaaaaca ttttataaaa aagattttga tgcctgttct
     5341 tttgatgaaa tcactgttcc tagtcatatt gaattagcaa attatgacaa gatccattac
     5401 atcaatacga tgtatccgtg ggagggacat gtatttcgcc gtcccccttt cacgacaagc
     5461 cctgagaaag aggcggaagg gatcttttca gctgctaaag acaatccggt tggttcgtat
     5521 ttgacgactt ttcgattaga agaaggtcta aagggaaaac aaatctcgat tcattttgct
     5581 ggtgtagaac aagcaatgtt tatttggttg aatggtcatt ttatcgggta tgccgaagat
     5641 tcgttcactc cgtctgaatt tgatttaaca ccttatttgg aagaagaaaa tattctagct
     5701 gtggaggttt acaaaagaag tacagctgca ttcttggaag atcaggactt tttccgattt
     5761 tttgggatct tcagagatgt cttcttagtt gctcgaggac aaaaccattt agaagatctt
     5821 tggatcaaac ctcgcttaga tgacaaatgt caaaatggca aacttgaagt agatttgtct
     5881 tttgagcaag tagccaaaaa cagtcagctg aatgtagcga tactagatcc tgatgggaag
     5941 gaaatctatc aacagcaata tccgttaaaa gagcaaatga cgattcgcat cgatggattt
     6001 gaaaatgtgg cactgtgggg gcattttcag cccaatttat ataagcttga attggagacg
     6061 atagacgaag aaaaaagagt aacggagtat attgactatt ccttcggttt tcgtaaaatc
     6121 gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga tgattatcaa cggggtaaat
     6181 cgacatgagt ggcatccgaa aaaaggacgc gccatcacta tagaagatat ggaaaaagat
     6241 ttggctatca taaaaaatgc aaagatcaac ggcgtacgga catctcatta ccccaaccag
     6301 atcacttggt attatttatg cgaccaagca ggaatccatg tgatggcgga aacgaacctc
     6361 gaatcccatg gctcatggca aaaaatgggc gcatttgagc cttcttacaa cgtaccaggt
     6421 gcagtacctc agtggcaggc ggctgttctt gatcgagcaa aaacgaattt cgaaacatta
     6481 aaaaaccatc caagtattct tttttggtct cttggaaatg aatcttatgc ggaagaaaac
     6541 attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg atcgacttgt ccattatgaa
     6601 gggaattttc caaatccagc attcgaagat tcgatttcag atgtaaaaag ctgcatgtat
     6661 gcgccaccag aagagatcat tgactacctt gaaaataaag cggaaaaacc gttcatctta
     6721 tgtgaatata tgcatgacat ggggaattcg ttaggtggaa tggattctta catgacgttg
     6781 cttgatcgtt atgaaatgta ccaaggaggc tatatatggg attttaaaga tcaggcgatc
     6841 caagtgactg atccaatcac aggcagagaa gtgatgcgct atggcggtga ctttgacgat
     6901 aaaccaagtg actatgaatt ctcagggaat gggatcgttt ttgcagatgg acaagaaaaa
     6961 cccgccatgc aggaggtaag atattattat gaaaaataca gtaaataaaa gtcatatgga
     7021 tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta ggtgtcaaat cggggaatac
     7081 gcattatatt ttttcttata caagaggcgg actggaatcg ctcaataaaa acggaaaaga
     7141 atggctatac cgggaaacac ttccagtttt ttggcgagca acgaccgata atgacagagg
     7201 tagcggattt ggatttaaat ccggtatgtg gttaggagcc ggattgtatc caaaagtcaa
     7261 agaaatagaa gtactggtgg acaataaaaa gatcgatttg ccgattgctc cagtaaataa
     7321 tcaatattca agtaatgaat acgctgaaac ggttgaaatc atttttacac tggaaatcaa
     7381 cacagtacca aaaacccaag tgacaatttc ctaccaaatc gctggagagg gtaaaatgct
     7441 tgtcaatatg cattattatg gacaaaaagg attaccggaa ttacccgcat tgggtttaag
     7501 gttcattatg ccaacgaaag caaaattttt tgagtatgaa ggcctatcag gggaaacata
     7561 tccggatcgg atggctggtg gtgtcgaagg tatttataaa gtggcaggac tccccgtttc
     7621 cccatacctt gttccccaag aatgcggcat gcatatggaa acgaaatggg tagaactaac
     7681 aagaaatcaa acgttggata atacggataa agatcaagaa gatttttcgt tgcgatttga
     7741 acagcaggat acgccatttg ctttttccgc tttaccattc aaagcagaag aactggaaca
     7801 ggcaacacat atagaagaac taccgccaga aagacgaacg gttcttacga tttttgcaaa
     7861 agtaagagga gtcgggggta tcaatagctg gggagcggat gttgagtcac cttaccgaat
     7921 cagcagcgaa gaggaccatg aaatcggctt tattattcag tagcaatctt tagattatta
     7981 tttgaaaaac ggagt
//
LOCUS       DAZUKR010000118         7928 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000118 DAZUKR010000000
VERSION     DAZUKR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.120"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..812
                     /locus_tag="ACG89C_002272"
     CDS             111..812
                     /locus_tag="ACG89C_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4900794.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1421..2437)
                     /gene="tsaD"
                     /locus_tag="ACG89C_002273"
     CDS             complement(1421..2437)
                     /gene="tsaD"
                     /locus_tag="ACG89C_002273"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4900795.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2434..2898)
                     /gene="rimI"
                     /locus_tag="ACG89C_002274"
     CDS             complement(2434..2898)
                     /gene="rimI"
                     /locus_tag="ACG89C_002274"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4900796.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2905..3447)
                     /gene="rimI"
                     /locus_tag="ACG89C_002275"
     CDS             complement(2905..3447)
                     /gene="rimI"
                     /locus_tag="ACG89C_002275"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4900797.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3431..4153)
                     /gene="tsaB"
                     /locus_tag="ACG89C_002276"
     CDS             complement(3431..4153)
                     /gene="tsaB"
                     /locus_tag="ACG89C_002276"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4900798.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4344..5324)
                     /locus_tag="ACG89C_002277"
     CDS             complement(4344..5324)
                     /locus_tag="ACG89C_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4900799.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5348..6832)
                     /gene="galT"
                     /locus_tag="ACG89C_002278"
     CDS             complement(5348..6832)
                     /gene="galT"
                     /locus_tag="ACG89C_002278"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS4900800.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6844..7833)
                     /gene="galE"
                     /locus_tag="ACG89C_002279"
     CDS             complement(6844..7833)
                     /gene="galE"
                     /locus_tag="ACG89C_002279"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4900801.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2230 a         1700 c         1403 g         2595 t
ORIGIN      
        1 atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt tacaaattct
       61 ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta atgcgtgtta
      121 ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa catttctcgg
      181 atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa aaaggacaga
      241 tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa ggactcgttc
      301 gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg aatacaaata
      361 acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca gctcaagcga
      421 tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta gcacgaaaaa
      481 atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa atacatgaat
      541 tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa aatttattca
      601 ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt ccttatccga
      661 tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct ggacaagtaa
      721 tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt actttctgta
      781 agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca gtgaccctga
      841 taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt
      901 agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta atattcactg
      961 tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc cgatctagat
     1021 tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag gaacggataa
     1081 tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta tgtaaaaaat
     1141 agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt acctgtcaac
     1201 tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga aattgttcct
     1261 tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt tcaaaagctt
     1321 gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt tcctgggctg
     1381 ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat aaaaccaagc
     1441 ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc acatgtgctg
     1501 ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc actagttcta
     1561 cctcagggag tttagctgcc aaagcatcct gcaatccttc acgcaaccct tgattggccg
     1621 ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct cgaagtgttt
     1681 tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca tttttgtcta
     1741 gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt tttaatccgc
     1801 taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg taattatctt
     1861 ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga cctaatactc
     1921 ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca atgatttcat
     1981 atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca agaagtgcca
     2041 tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct gccatatggt
     2101 ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca gaaataccaa
     2161 tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat aaatcttctg
     2221 ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt tctacgtgat
     2281 ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt atttgagaag
     2341 caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc gtttcatcac
     2401 aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg tttttaactt
     2461 tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc gtttacctaa
     2521 tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata gacgtacttc
     2581 taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta acaaatgccg
     2641 tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct cgacttcatc
     2701 tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta aaagcagata
     2761 atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg
     2821 tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt catcaaaatc
     2881 tttttttctg caaatcaact aatctcactc attttctaaa agttttatca tatccaacgc
     2941 atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa accccaattt
     3001 ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca tcgtctcaca
     3061 gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga ttcgtttacg
     3121 ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga acgctcttga
     3181 accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat gaggaagcgg
     3241 tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat tatagacttc
     3301 tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta gtacgtaacg
     3361 acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa aaaagcttct
     3421 ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc
     3481 atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa
     3541 ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata tcccatgctg
     3601 gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag aatttgtgac
     3661 aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg aacaaatcag
     3721 aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc cagcggtatg
     3781 ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata ttatccacac
     3841 cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct ttatctaatg
     3901 tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa gaaccgggac
     3961 catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct gcaaacaatt
     4021 ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt tgtatttgtc
     4081 caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca gtatctattc
     4141 ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc attgtagcat
     4201 acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa tacatgatta
     4261 tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg tatcactcca
     4321 ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa ctctgtttcc
     4381 acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat cgtctccact
     4441 gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg cgttacatat
     4501 ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac cactaaacct
     4561 ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc aaaaagcgca
     4621 tcaggaacat gcggattcgt ttttaaatat tgcttcactg cttgatatcc tgactcgaca
     4681 gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa aacttgttga
     4741 aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata ttctgttcca
     4801 gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac tttttgaaca
     4861 cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc agaatctacc
     4921 aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc gaattttcct
     4981 agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc tttcaataaa
     5041 gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa ataataaaga
     5101 tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg cttagtattt
     5161 gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa aattttttgc
     5221 tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga aacagtagca
     5281 ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct ctcctccttt
     5341 tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt tcatctcgct
     5401 tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat ttttgtacga
     5461 catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt tctgcccacg
     5521 gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct acttctacta
     5581 gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc aacccaatgt
     5641 tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca ggatgttctt
     5701 ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc cttcttcgtg
     5761 caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg atagatactg
     5821 tttcatccga atatgctttc catttttcca atactgagtt agctgcttca ataagcaagt
     5881 ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact cctgcgatga
     5941 cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc ataggaaatt
     6001 catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca attggtaagt
     6061 cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc agtctagcaa
     6121 atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc gaatgttcat
     6181 tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga ttcattcgaa
     6241 taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct tcgttttcca
     6301 tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct gcgatttgtt
     6361 ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca tactccgact
     6421 ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca tttttcttac
     6481 ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc tgtgcaaaga
     6541 atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct tcaaaaatct
     6601 cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg acaagctgat
     6661 ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct agagcatctt
     6721 ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc atccatccgc
     6781 cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac atatctcatt
     6841 cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg ttttgataat
     6901 cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt tttcacttga
     6961 agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg gaatttcttt
     7021 tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt tgctgctgcc
     7081 taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta aaatatgtgc
     7141 agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg gtgtatcata
     7201 atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta aaatgattgg
     7261 gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt ttgctccggc
     7321 tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac accacttcat
     7381 cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag ctggtgtatc
     7441 ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag agaatacgat
     7501 tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt atacgttgtt
     7561 gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg cagcaaaatg
     7621 gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt tatcccggat
     7681 gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg tgagcaaatt
     7741 atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt gcgaaccgat
     7801 ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga ttagtaaaat
     7861 tgtgtttgtt gattctatat aatttaaaaa acgaagttta cttttatggt gaaaacaaac
     7921 aaagtaaa
//
LOCUS       DAZUKR010000119         7866 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000119 DAZUKR010000000
VERSION     DAZUKR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.121"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACG89C_002280"
     CDS             complement(21..995)
                     /locus_tag="ACG89C_002280"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4900802.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACG89C_002281"
     CDS             complement(1075..1950)
                     /locus_tag="ACG89C_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS4900803.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACG89C_002282"
     CDS             2080..3987
                     /locus_tag="ACG89C_002282"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS4900804.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ACG89C_002283"
     CDS             complement(4528..7806)
                     /locus_tag="ACG89C_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS4900805.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2217 a         1662 c         1462 g         2525 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttt
//
LOCUS       DAZUKR010000120         7807 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000120 DAZUKR010000000
VERSION     DAZUKR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.122"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..2347
                     /locus_tag="ACG89C_002284"
     CDS             20..2347
                     /locus_tag="ACG89C_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS4900806.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2344..3258
                     /locus_tag="ACG89C_002285"
                     /pseudo
     CDS             2344..3258
                     /locus_tag="ACG89C_002285"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3331..3846
                     /locus_tag="ACG89C_002286"
     CDS             3331..3846
                     /locus_tag="ACG89C_002286"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS4900807.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3843..4289
                     /locus_tag="ACG89C_002287"
     CDS             3843..4289
                     /locus_tag="ACG89C_002287"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4900808.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4439..5254
                     /locus_tag="ACG89C_002288"
     CDS             4439..5254
                     /locus_tag="ACG89C_002288"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4900809.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5460..6632
                     /locus_tag="ACG89C_002289"
     CDS             5460..6632
                     /locus_tag="ACG89C_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4900810.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6794..7501)
                     /locus_tag="ACG89C_002290"
     CDS             complement(6794..7501)
                     /locus_tag="ACG89C_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900811.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
BASE COUNT         2640 a         1463 c         1547 g         2157 t
ORIGIN      
        1 ttttttattg agaggaggga tgaaaatgat taagaaaact caaaataaaa tccaacgtat
       61 atgtcaaaaa cattttgttt ctagactcaa agctgtatct aaaagaaaat acttacatcc
      121 cattatgatt tcgatcatct tgatttttac tttcttaggg agcttatcaa gtatttatgt
      181 aaacgtttat acagaaacgt caaattttgc taaccaaacg acagaggaga caaatagtcc
      241 accctattct gaaatacagt ccatagaaag caccaatcag tctagcaaca acgagacaac
      301 ctcaaccaca tcctcgactc aactgattga tccgacagaa aaagaaatca cagcagcaaa
      361 agcagcaggg gagaaagctt attcagaaac aggtagaagc cagacattta gtactgttgc
      421 ggccactgcg aatttcaacg gacataccat accagatgga tatacagtga tatatgccaa
      481 agatggagtc attgttgcgg ttaacggatc gatgagtgcg gataaaactg cgttacaagg
      541 cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat atccataatc tgtttattgg
      601 ctccacagta aatggtacta ctatcacatt cccaaaaagt atctatatgg gggtggctac
      661 cgctggaaat acgatcgatt cagttacacc caatgcggat aaaagttatt cttttacacc
      721 ctcaacatgg ggagggcttc ctgccggagg aggtatgagt actttttcaa tcggtataca
      781 gttagcaaat gatattccat tcacggggta ctcgatcaaa aatgggcaga tcgttgatgg
      841 taagggaaat gtttcaaatt ttccaaccta tcaagcagca acggttcagg tcaatgcgaa
      901 tcggacagga actttttctt atacagtcaa tggtggagca gcaaaaatcg gaacgtacgg
      961 gactacatta tctggaatcc atgtgggtga caaagtggtt atcactccaa cagcagtaac
     1021 gggctattct tttgcacaaa caaacggttc tttcacagcc ctagaaagta acgtcgatac
     1081 ggtgacctat acaggcaaac aaacaccaat cactattaag tatcaagact attttggaca
     1141 gatgattgct ccttctaaaa taatgaatct tacttacgga agtgctgcac aagacctgac
     1201 gactaatacg cctactatca acggatatac ctttacagct gtttcagcga tagaaacaaa
     1261 gaaccaatca gcaatgagtg ttttagctag tttagataca aatggaaatg taatcgtaaa
     1321 ggacgcaaat ggaaaacaga tttctgaagt catcctatat tataagacaa atgcaacagt
     1381 atcaatcaat gcaaatggca gtaagtacta tgatggtctt tctgttgtac cgatcgttaa
     1441 atatgccttt aatgataaaa cagaatcaac atccctttta aacaatgatc tcagtattga
     1501 tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat gaaaagggag ttgaagtaac
     1561 tgcgcctact gaaattggaa cctatacgtc ttggcaattg acaaaaagcg gactagctaa
     1621 actagctgcc agaacaaact atttatttac tatcgtgcag acatctgatg tctatacgat
     1681 taagcaaatc agcggtgcgg tagagctcag tggcagtaag acatatgatg gaaaagcagg
     1741 tgtcccgtca atccatgtca agctagcaga gggtgtcact agttcgctta ctccagtaca
     1801 agtggcactt agcagtatgg attacacagc agatgcacaa tcagcaaaaa atatggttaa
     1861 tgctggcagc tatacaatta aactaacgaa tagcggaata gacaaagtca aagcagcaaa
     1921 tcccaagttg tcatttactg atatagccaa cacatcagga acttatacca tctataaagc
     1981 taacgcagtg attactgtcg atgacgctgc ctttaattat gatgcccaaa gtcactcgat
     2041 ttcggttgga aatctccacg ttaccggtgt tgtcttaggc gaatcattgg attataccct
     2101 aacagttaac agccggacag atattggatc acaaatagta acgattagtt taggcgaagg
     2161 aagtgtaaac aataattata cgattatcgc aacagatacc gctaagttga cgatcaatcc
     2221 gactctcttg cttccttcga caggtgggat aggtctactg ccctttgtct ttttaggact
     2281 gatctttatt ttttctggct tcttttattt tatccatcgt aagaaaaaag caggtgaaca
     2341 gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg ctgttctctt ttttatccta
     2401 ctggtctttt ttataggttc tccgactgtg tcagctagag aatcacctat tacatttact
     2461 ttgcataatc gaatgttcga aagcgataag atgccaagta tcagtcaaaa cgacggaata
     2521 aatatcccgg ataactttca aggagtaaaa gggttgaacg gcgttacttt cgctatatat
     2581 gatgtctcgg atgaatttta caagctgcgg tcagaaggaa gtagtgtgga agacgcacaa
     2641 agaaaactag cacaaaaaag tgatagtgaa aaaatactgg cagaaaatgt gacaaaaaca
     2701 gtagacgagg aagagggcat tgcttctttt agtgcatcgg acaaagatga gcaaggtcgt
     2761 gacgctgttt accgatttac ggaaataaag acttcagatt aagtaaaaga aaaatcagca
     2821 ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc ataaattgac gaccatccac
     2881 ttgtatccaa agagcgaaca aaaaatacct gctgcactca cattaacaaa aacagtagag
     2941 aataagcaga cagattttgc agatggggat aaagtccctt atctcatcac cgtaacaatt
     3001 cctgagaaca tcaacgagat aggaacctat acgatcaaag atacagctga tcctcagtta
     3061 tggctagaac cagaaacgat cgattttttg attggtggag acaagattca tacatttcac
     3121 acacaaaaaa taaaacatgg ttttgtctta tcagattctg gaaaagatct tagttcgttt
     3181 gctggaaaaa aattaacgat ccgttaccaa atgacattga aagaaaacag tgaaaaaacg
     3241 acatttgata acgatgtacg tttgacgact gacaatcaaa cagtagaaac acatcttgcc
     3301 atccaaacag gaggcaaaca atttgtcaaa gtggatcggc aaacgaaaca aaaactagca
     3361 gatgctcaat ttctagtcaa aaaaaatgac caatacttag cgcaagaaaa cgggataaat
     3421 cattgggtag ccaaagatga ttcaagagca acgattttta cttccagtaa agatggaaca
     3481 ttttctgtcc atggattgtc ttttggaagt tacgaactag tggagattaa accgcctaaa
     3541 ggatatatat tgagtcaatc aaccattctc tttgaagttg aaaaaggcag ttatgatccg
     3601 cttcatccta taccattgga aatcatcaac gaaccaaaag caaatccgac gaataatgga
     3661 aaaacgccag ttcagatgca gacaaatggt ccatcaaatg aaaaaaatgg aggaagcttt
     3721 ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt tagggttatt gctagtcctt
     3781 tcaacaggga cagcttggtt ttataaaaaa caacaaaaag gaaacaatag gagggaaata
     3841 aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg aactgctatc atgctgcttc
     3901 ctttcgttct cggcctagga actgcagagg tttctgcagc cgtatcacct acaccagaaa
     3961 atgttacggt taatttgcat aaattgaaat tcacttcagc accagaaaat caaataaaca
     4021 acggaacgga attgacattt ccgaatagcg aaccgttgaa cggcgttgaa ttcaacgtat
     4081 atgacatcac tgctacgtac tatccaagta aagacacagc agttccagca gacgctacac
     4141 ccttcgcaag tgtaacgact tctggtgaag gattagctaa cctgactctt ccaggtaaat
     4201 ctgatgggaa agatgctgta tacgtctttg tagaaacacc aaagcccggt gtcgaaacgt
     4261 cgccaaatat cgtgttaagc ttaccctaga ccctgacaaa cctttagaca cgattcacct
     4321 atatgctaaa aatgtcatca aagaaagcaa tatcaaaatc cagaaagctg ggaacttttc
     4381 aggggtagat aagttagcag gggcgcaatt ccagatcaaa aattcagaga atcaattcgt
     4441 gaccggtttt gatgaaaaca ctggtgtcgc tctctatgat cctgctgagt cagcgttgac
     4501 attcacaact gatactgaag gatccatcag tgtcaatgga ttaatagacg gcaattatac
     4561 tttagttgaa acaaaagcac cagatggtta tcaattaacc aatccacaaa aagaaactac
     4621 ttttatagta aaaaacggaa aattgcaaca aacaaccggt atcatccatg gcgcagatga
     4681 agccaatctt gggaatccaa acggtgtagt caatcgggaa aatacaacta cggataacac
     4741 gattactgta gaaaatgtcc aaaactacgg tgatttccac ttcatcaaac aagatgtgaa
     4801 cacgcaagaa aaattagccg atgcagaatt ttctattcta agagcaaaag atccaactgt
     4861 taaattaatg aaaaaaatag caaaccttgc ctcaggagaa tacccatatg cttggtcaga
     4921 tgaagtcaca ggttctgact gggaagaagt gaaattgatt tctgattcag aagggaaatt
     4981 ttcaatcgaa gggctgaaac aaggggcata ctgccttcaa gaaacaaaag caccagaagg
     5041 ctacgcatta cctaagaatg aagctgcgta cactgaattt acagtagatg atgatccaac
     5101 aacttctgat gaaacaacga tcgataacca acctaaagga atcttgcctt caactggtgg
     5161 tatggggatc atcgtattca tcttggtagg tactgcttta gttggaggag cagtgatcta
     5221 tttcaaaaaa cgccatcaag aaacagaagc ttaaactatt ggaaacaaaa atcatacgat
     5281 aaataaacga tagtgattca aatggggaga aaggttgtga caagtggtcg gtctcttggt
     5341 attcgctcaa caaacggcaa aataggagga actattttgc cgtttgcttc gctaatactc
     5401 aggtcaaggc gctgatttca caacctcttc atatacatat tagaaaatag gtggacatga
     5461 tgaataaaag cagaataaga atactggaca tcttcatggc aatcatttta gtcgttggga
     5521 tcgggatctt ttcttatcct tttgtagaag attcactgaa tgattttctt gcccagcaaa
     5581 tgatcatcca ttatcaaaag caagcatcga ataagaattc agaggaaata aaaaaacagc
     5641 aggaaaaaat gacaaagaaa aaccaacagt tggcagagca aaacgtatca ccagggatag
     5701 gcagtttcaa ccaagcagtt gatgcaaaag cattaaaaga tttacctagt aatgcctttt
     5761 ttatggaaca tatgcttgga gtcatcgaaa tcccaaaaat caatgtcagc ttacctatat
     5821 tcgatcagac aacggaaata tttttgcaaa aaggaacgtc attattggaa ggaagttctt
     5881 atccgactgg cgggaaaaac acccatgccg ttctttctgg gcatcgtgga ctcccagaag
     5941 caaaattatt tacagatttg cctaagttga aaaaaggaga ccagtttttc atccagatca
     6001 atggaaagac attggcgtat caagtagaaa aaatccaagt ggtcttacct gacgaagtag
     6061 attctttggg tatccaaaaa ggcagagatc tcgtgacttt gcttacctgc actccttata
     6121 tggtcaatac acatcgactg cttgtgacgg ggcatcgtat tccttatcaa gcaaaagagg
     6181 caaaaaaagc gatccaagga attgatcaat ggaaaaaatg gaaatttttt atttggttta
     6241 tcggtatact acttggatca atcggcttag tttggctact catcgcttat ttagattctt
     6301 tggccatcgc taagcgaaac tatcctttat ctttttatgt aaagaacaca aacggacgac
     6361 caatcgaagg aatggtcttt tctgtaaaaa cgctcaacgg caaacactat attactagag
     6421 aaaaagttcc atttgtgaaa gcttctgatg aatacggact agtgagattt tctgatttaa
     6481 aaggcaggaa ctatcgcttg caacatgagg agctcctgct taaaatccat gtaaaacata
     6541 aacattcgaa acagttttca atgaaattga aaaaaggaag atataaactg cgaaaagaaa
     6601 aagaggtgta ctatttgatt gagaaagaat gaatgaagat gtccaaaaaa gcagagggaa
     6661 cagactaaat gaaaaaagag agaagctgac gaaatggacg tgtaaactgc tatttttggt
     6721 ttactatctg gaacgccagc ttctattttt ttatgtttca gaagaatttt attattatag
     6781 tataactttt gaattagttt ccataacatt tggagcaagg ggttaaacca cgagcttgag
     6841 cttcgcttaa agtggcaggg gtatacgtac catttccaca tttatgtgta tggtattttg
     6901 aaccggtcgg tgtgacatag acagtttgtt cattattttg ctcaggagct gcggcttgtg
     6961 cctgctgaga agcctgttca gctgctgctt gctgtcgcgc ctgttctgct tgttgctgtg
     7021 cgattgcttc ttgcctcgcc tgttctgctt gttgctgtgc gattgcttct tgttgcgctt
     7081 gttctgcttg ttgtcgtgca atttcttctt gctttttccg ttcttcttct gcttcttttg
     7141 ctttgattgt ggaatcaatt gcagcaagac ggcttgtcaa ctcttgtttg ttttcaggta
     7201 gcgcttggat agctgctgaa gcagttgcat agttttcttt tgtaggattt gcttcagctt
     7261 gtgacaaagc agattccgct tctgtcactt ttttttctag tgcagctttt tccgcttttt
     7321 gtttctcttc ttcttttttc ttttcaattt gttctttcga tgaatctgcc gtactttcgg
     7381 agatcgtggt actgctgctg gaagcctgag ctgaacttgt gctttgagta gtagacgtag
     7441 tagcaccaca cccactcaac aaacttcctg cgaccaaaaa caacgcaact aattttttca
     7501 ctaaatttcc tccttaagta aaaaacaacc gttttcaata aatgtccttt tcaattgttt
     7561 acttacttgt tataataatt gtgttatgaa tagtgattga ggaaacttaa tctaatcccg
     7621 tgttgataca cgggattttt tgctatagtt ctgttattaa gtatgcaata ataaaatatg
     7681 tacgttattt tattattaga ttattgacat ttatataata aacgattaaa tgattcatat
     7741 caatagaata cttaggaaaa aaagagaaat aataacaggt ttatgcacta ttgacggtgt
     7801 gacaata
//
LOCUS       DAZUKR010000121         7782 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000121 DAZUKR010000000
VERSION     DAZUKR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.123"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..341
                     /locus_tag="ACG89C_002291"
     CDS             <1..341
                     /locus_tag="ACG89C_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900812.1"
                     /translation="FSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPK
                     SISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKN
                     NNFYFYRNKL"
     gene            complement(467..1144)
                     /gene="nth"
                     /locus_tag="ACG89C_002292"
     CDS             complement(467..1144)
                     /gene="nth"
                     /locus_tag="ACG89C_002292"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4900813.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1151..1849)
                     /locus_tag="ACG89C_002293"
     CDS             complement(1151..1849)
                     /locus_tag="ACG89C_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4900814.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2117..3301
                     /locus_tag="ACG89C_002294"
     CDS             2117..3301
                     /locus_tag="ACG89C_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS4900815.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3307..3750)
                     /locus_tag="ACG89C_002295"
     CDS             complement(3307..3750)
                     /locus_tag="ACG89C_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4900816.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3976..4263)
                     /locus_tag="ACG89C_002296"
     CDS             complement(3976..4263)
                     /locus_tag="ACG89C_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4900817.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4434..5786)
                     /locus_tag="ACG89C_002297"
     CDS             complement(4434..5786)
                     /locus_tag="ACG89C_002297"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS4900818.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6059..6505)
                     /locus_tag="ACG89C_002298"
     CDS             complement(6059..6505)
                     /locus_tag="ACG89C_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4900819.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6741..7667)
                     /gene="cysK"
                     /locus_tag="ACG89C_002299"
     CDS             complement(6741..7667)
                     /gene="cysK"
                     /locus_tag="ACG89C_002299"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4900820.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2103 a         1583 c         1270 g         2826 t
ORIGIN      
        1 ttttttctgt acttttaagc actactctta aaggaaaatt tatggaaaga agctctcatg
       61 caatgattct cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc
      121 tgaaaccgcc caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac
      181 ttatcatttt tattacacct ttagatagat acgtcactta tttatataat cctttggaat
      241 actggactta ttttataggt atattggaag gcattatttt ctacatcggg tattttccca
      301 ataaaaataa caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta
      361 ctgagtatgt acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata
      421 ataacaagat aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt
      481 tcttttttag cgccttttct tttagacgca tccgattttt cccatcttga catattgata
      541 gcaaaggaca tacctcgcac ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa
      601 tcagtgtatg atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt
      661 ctacttccat gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca
      721 cgtgggtatc tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg
      781 tctttctgcc tacaccagga agagacatca attcttctct tgacgttggc acttgcccat
      841 cgaaacgttc aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca
      901 aaccaatcgt ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg
      961 gtgtcggaaa agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct
     1021 gggcacttaa gatcactgca atcaataact caaatggatt attgtgcttg agttctccat
     1081 gtgcttcggg aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta
     1141 acatattccg ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt
     1201 gagctgactg ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt
     1261 ccacttgttg ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt
     1321 aattaaaact ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg
     1381 gctgataatg gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg
     1441 ggcgtccaaa ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt
     1501 tttcttcgta ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt
     1561 aaagattata tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc
     1621 catttgttac gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca
     1681 tagcaatcat ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta
     1741 gaacaaataa aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat
     1801 tagatatgac tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc
     1861 cgctcaatca cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt
     1921 tacttcactg atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt
     1981 ttgatgcgta tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga
     2041 atgttgacaa ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc
     2101 ataaaggagt ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt
     2161 ctctgtctta ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac
     2221 gctgccttat tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt
     2281 ttatgctcta gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg
     2341 gcgtcatccg atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg
     2401 tttaggaaat tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg
     2461 agaaatctcc accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa
     2521 agtttttgga tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg
     2581 gggtctatta gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt
     2641 cttaaatgcc gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc
     2701 agtagattta agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat
     2761 ttcatccgtt attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct
     2821 tcaatctatc ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg
     2881 tttaagcttc tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg
     2941 attgctcaaa aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct
     3001 cgcctatcta ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg
     3061 gattatctgt tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc
     3121 caattctgtc tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc
     3181 tctatcgcgc gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct
     3241 tccgacggta ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata
     3301 gtagcttcat ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt
     3361 gtcaaattct tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg
     3421 atggagttgc tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat
     3481 ttctttttta ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt
     3541 tgttagctta ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt
     3601 aggatcaggg attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc
     3661 aaattctttt tccatttctg gtttattacg atgaataatc tcattagcta aatccagata
     3721 ttcttctatt agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt
     3781 gtttttttgt ttcctattca acaaagataa aaatattttg taggaaaacc caatctgaaa
     3841 ccaagaaaaa aaggggttga caaagcttgt cataacccct ttttctgatt aaaagatagt
     3901 aaagagacag tcatcggtaa ttgtgatgat tttttgtcat cacttaagga tgagcggcct
     3961 tttcaaaaac tgtctttaaa gatcatcatc agtttctagc acatatggct gaccttcata
     4021 tgtgtactct gtattcaatg tgccgtcatt ttcatctgag tagatgcgtc tactgaaagg
     4081 aaaacgatga gaaacattat ctttggcgtc atttgctggc atcgtgtaat attcctcttg
     4141 cgtttcattc aactgagaga gtacttgatc tgccaactca tcaatattga tcgttaattt
     4201 cttcgaagca cttttataat cttctaaaaa tgcatcttgt tggacaaaat attctttatt
     4261 catcttcttt ctcacctttc ttttcaaaat caaataaagc tgcttgttct tttatgtaac
     4321 agctttatct ttgctgctta ttgtgacaaa aaattcgaag aaagacaact aattgtactt
     4381 ttcacaaaaa aagacagagg gtcgtgacaa aattgtgtca caaccctttt tctttattgt
     4441 gcagtttcga tttcgcctag cgctgcttgt gcaagtaggt tcaagtaatt ccatgggcgg
     4501 tcaaaatgtg gttggaagaa gaaatctgaa agtgcgagat cttctacagt cattttattt
     4561 tgaacagcta aagacatggt atttgctgat tgtgtaatgt cgtatttgga catgaactga
     4621 gcacctacga tacgattcgt tcctttttca tagactagct ccatcattac tttttcagta
     4681 gttggcatga actctggacg ataattatct tcaaatacag tcgcttggac ttctagatcg
     4741 ttcaaagttg cgctttcttt ggtcacacct gtcgaaccga tcgtccagcc aaataggtaa
     4801 agtcctgaag ttccttgagt accacgatac gccattttat gatctgtcaa gttatttccc
     4861 acaagcaaac cttgacgaac ggcatttgtt gccaaaggaa tatagttttg cgtgccactt
     4921 ggattgtagt tgactactgc agagtctcca gctgcaaaaa tgtctggatt acttgtctgc
     4981 atgtattcat tcactttgat tgccccgttt ggcagtgtat ctacttttcc ttcgatcagt
     5041 tttgtatttg gacggaagcc cacacataaa atgaccatat ctgtatcaaa tgtatctgtt
     5101 ggtgtagcta cttgtttcac ttttccttct tcatctgtga taaattcagt aacattttcg
     5161 cctagcgcaa gagtaacacc gcggtctgtc aattcttttt ctaagatatc tgtgaatggt
     5221 ttatctagat atttattcaa gatgcgatct aatccatcaa tcaatgtcac ttgttttcca
     5281 gattcgacaa aagcttctac taattcaata ccgatatatc caccaccaac aattactaca
     5341 cgttttgctt ctttcgcttg tgcaataatt tcatttgctt gattgtagtt tttgcatagg
     5401 agtacatttt tcgcatcgat tccactaatc ggtgggataa taggccatga accagttgtc
     5461 atcactaatt tatcataaga aaccgtttct gtttctcctg ttttcagatc ttttgccgtt
     5521 actgttttat tttctgtatc gacgtctgtt acatcgtgtt ccattttaac tgttgctcct
     5581 aatgaagata attcttcagg atttgagtag aacaagcctg ctgggtcctt tactactccg
     5641 ccgacataaa gtgcgatacc gcaagataag aaagaaatat tgtcatttct ttcatatact
     5701 gtcacttctg cttctggatg attttttaaa atacttttta cagctgcagt acctgcatga
     5761 gtacagccaa ttactactac tttcatttat ggtccctccg attaacattt tgtccttcga
     5821 caaaactact ataacagaaa taaaacggat acaacagttt aatgcttgtg aattcacaaa
     5881 ggtttgtttg tagtttcatt ccttttcaag cataagaacc caatatctac aagattctta
     5941 ttttaataat attcactttt atatctaaga ttgtgaataa aagacaaaaa aataaaaaag
     6001 ctagcgtttt ttttacacag ctagcttttt ttaggtataa cacttgtaga aactgttttt
     6061 acatttgcgc cttttttgct gactctttac aatccggaca gagtccatac aattccaacc
     6121 gatgttcatt aatctcaaaa tcagtcagtc tgcttgcagc catttctata tcttcaagcc
     6181 ctgggtaatg aaaatcaaca atcttaccgc agttttcaca aattacatga taatgacgct
     6241 ttgaactgaa atcaaatcgg ctagaagcat cgccgtactt catttcttga acaaatccaa
     6301 tcgaagtaaa taagcgaagg ttattgtaaa cagtcgcaac actcatattt ggaaaccgat
     6361 gttcaagtgc tttataaatc tcatcagccg ttggatggga atgatgttca atcaaatatt
     6421 ctaaaaccgc gtaccgttga ggtgtgattc gaatgttctc tttttttaac ttttcgataa
     6481 cctcttcgac taacttattt tccatgtaaa caccccacgt tgttattctc gtcttatcat
     6541 actattttta cattttaaaa tccacttaac atgaaaagaa aattcaattt tattgtattt
     6601 taatgaaaaa agacgtaaaa cagtcgcgtt cactattcta cgtctctatt caaataattt
     6661 ttctcatcat aaatttttgt gtgttctttt catctttaaa agttttttac ataaacctat
     6721 ctggagaaaa accaagtgcc ttatatctct tggtaaaggg ctgtagataa ataacgttca
     6781 ccattgtcgg gaaccacagc taataccttc ttgccttttc ctaattcttt tgcgactttt
     6841 agcgcggcag caattgcagc gccagatgag atgcctacta aaatcccttc ttttcgccca
     6901 acttctctag ctgtttccat cgcttcgtct ccactaatag aaaggacttt atcatacaca
     6961 gaagtatcca gtgttttagg aacaaaacct gtaccgattc cttggatttt gtgaggtccc
     7021 gcttctttcc cttctaaaat agcagattct gctggttcta ccccgattaa ttcgattttc
     7081 ggatacacac gttttaattc tcggccagcg ccagtgatcg ttcctcctgt accgatacct
     7141 gcaacaaacg cgtccaagcc atttactcca aatgcttgat gtatctcagg accagtcgtc
     7201 ttctcatgca caagaggatt tgcttcattt tcaaattgta atggtaaaaa gtagccgttt
     7261 tcttttgcca aacgttctgc ttctctgatt gatccagaaa taccatccgc accaggagtc
     7321 aaaatcaact ctgcgccgta ccctttcatc aataaacgtc tttcgatact catcgtctca
     7381 ggcatcacga tgattacttt atatcctttt gcaactccta ccatcgacaa tccgattcct
     7441 gtatttccag aagtaggttc aatgatagta tcacctggtt tcagtagacc atcgtgttct
     7501 gctttttcta tcatacttaa agcaatacga tcttttacac ttcctcctgg attaaaaaac
     7561 tcaagcttta caaaaacatc tgctgaatct tctggtacga ttttatttaa tttgacgatt
     7621 ggcgttttgc caatcaattc ggtgactgag ttatagatag ctgtcatctt caattcctcc
     7681 ttgtctttgc tcttcttact tatcataata caatctgttc atttatggcg agtgattcgt
     7741 ctttgactag caaaaaaaag acaaagatat accatatatg gt
//
LOCUS       DAZUKR010000122         7616 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000122 DAZUKR010000000
VERSION     DAZUKR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.124"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..1093)
                     /locus_tag="ACG89C_002300"
     CDS             complement(83..1093)
                     /locus_tag="ACG89C_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4900821.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(1105..2058)
                     /locus_tag="ACG89C_002301"
     CDS             complement(1105..2058)
                     /locus_tag="ACG89C_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900822.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(2048..3118)
                     /locus_tag="ACG89C_002302"
     CDS             complement(2048..3118)
                     /locus_tag="ACG89C_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900823.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(3124..4167)
                     /locus_tag="ACG89C_002303"
     CDS             complement(3124..4167)
                     /locus_tag="ACG89C_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4900824.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(4168..5109)
                     /gene="opp3b"
                     /locus_tag="ACG89C_002304"
     CDS             complement(4168..5109)
                     /gene="opp3b"
                     /locus_tag="ACG89C_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4900825.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            5193..5540
                     /locus_tag="ACG89C_002305"
     CDS             5193..5540
                     /locus_tag="ACG89C_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS4900826.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(5610..7271)
                     /locus_tag="ACG89C_002306"
     CDS             complement(5610..7271)
                     /locus_tag="ACG89C_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4900827.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            7450..7569
                     /locus_tag="ACG89C_002307"
     CDS             7450..7569
                     /locus_tag="ACG89C_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4900828.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         2136 a         1576 c         1379 g         2525 t
ORIGIN      
        1 gaaaaattcc tagaaacaaa agtcattaag actttttatt aacaaggcta accctaacgc
       61 tcctatcagt ttcattcatt ttttatccct tgataaccga atgtctgccc attttttcta
      121 ctacttggct ctttcttacg gaattgcttt cttctgctcc gtcggtattg atcaaaagaa
      181 attttttatt ataccattgg gacttataac gcaattttgt tattaattta tgccagtcgt
      241 gaatagaaaa atcagcatgt gcctcttctt ttggaaaaga ttctacaaat aagtagtcat
      301 attgtttgac caatttccca gataattgtt caagataatc ttctcgctgg ttcttttgtt
      361 tcagataaag atccattact tttctctttt gtttctgata attgcttgcc tgatctaacc
      421 tgatctttct atggtgagcg gagcggactt tcacattcaa tttccgctgt gcatgttgca
      481 gcttttcttc tgtctggtga aattttggta aagttacttc tagaggtcga gatgtttgga
      541 tcaatgtctt cggatgatag gcgatcccga tccatttagc taaagattct ttttttactg
      601 gcgaaacatc acaaagtagt gcgacataaa attcttcatt atatcgagca gaaatcgtag
      661 cggagcgaat cgatccttca actggccgat gctgatttat cttgattagt tctttttgtt
      721 ttggtaactt taagtagcca ttttccagat agatcgtacg ggtttggttg ttggtcgtat
      781 aagattgcca catacttttt ttcgttttaa gctttgggaa gctggcacgt cccttgaaat
      841 aattgcgaaa agcccgatcc aaattaagct gtgcatttgc taaagcaaga ctgtctactt
      901 cttttaagaa tgggtattct ttttttaatg tagcgggtgt gagcgaaaaa ttggctactc
      961 ctgtttgttg ccaacttttc tgccgctttt tcagcaaatg gttataggta aatcgagcac
     1021 atccaaaagt acgaatcaat tgctgttttt gttcttctgt cggatacata cggaatttaa
     1081 aagcttttag catctatgtc cctcctagtt agcaaccaga agagattcct tttgtcgttt
     1141 tagttttttt gcgtaatatg ggacttcctg ctcagttgca tagacaaaat gttctggtgc
     1201 gatttcacga agttgacgta cttgtccgtc atctggttca ggttgatatt tgattcttct
     1261 gcgttttctc tcatggtctg gatctggtag aggaatagca gataacaaac tttctgtata
     1321 aggatgaaca ccgtgattat agatttcatc acttgttccc atctctaata acaagccgtt
     1381 atgcattaca ccgattcggt cactaatatg tttaaccatc gacaaatcat gtgcaataaa
     1441 tagataggtc aattgatgtt ctttttggag atcttgcaac aaattgacca cttgggcttg
     1501 gatcgacaca tctagtgcag aaattggttc atcacaaatg ataaaacgcg gttttactgc
     1561 caatgctcgt gcaataccaa tacgctggcg ttgcccacct gaaaattcat gaggataacg
     1621 agtaccgtgg cttggattca atccaacagt tctcaataga tcatcgactt tttttgcacg
     1681 ctcttcagat gatttagcta gtccattaac atcaattcct tcggcaatga tatctctgac
     1741 tttcatacga ggattcaacg aagcgtatgg atcttgaaag atcatctgaa catcatgccg
     1801 gaatttcgtc aaaccttttt tatctttgat cttcgttata tcttgaccgt cgaataatat
     1861 ctcaccatca gtaggttcat tcaagcgaat gatcgtacgg ccagtagtgg attttccgct
     1921 tccagattct ccaactaaac cgaatgtttc tccttcataa atgtgaaagc tgatgtcatt
     1981 tacagcatgt acttcattct tttttcctac attgaaatac tgtttcaatc ctttaacttc
     2041 aagtagttta tttggcatca gcaattcctc cttgaccgta agtatgtttt tgtttctctt
     2101 taaagatagc ccagcgacgt tggatttctt ctggcggtgt cactttgggt gcctgcggag
     2161 caagtagcca agttgctgcc ttgtgtgttt ttgatagttc aaaaaatggt ggttcttgtt
     2221 ctgcatcgat tttcatcgcg aattcatttc ttggatagaa tgcgtctcct tttggcgggt
     2281 tcaataaatc cggtggagaa ccaggaatag catacaatcg atcgttcgca ctatctaatg
     2341 tcggcattga accaagcagt ccccaagtat agggatgttg cgggttgtag aaaatttctt
     2401 ccgatgttcc aacttcaacg attcttcctc catacatgac agcgacacga tcagcgacat
     2461 tcgctactac accaaggtca tgagtgataa agataattga tgtatcgatt tttgtctgaa
     2521 tttctttcag cagctccaaa atctgtgctt gaatagtcac atcaagcgct gttgtaggtt
     2581 catcagcaat caggatttcc ggataacata ccagtgcaat cgcgatcacg atccgttgac
     2641 gctgacctcc tgagaactga tgcggataac tgttgatacg tttctcagct tcgggtattc
     2701 caactaattt aagcaattct aatgcagctt tttgtccctc tttttttgaa actttattat
     2761 gctttctaag ggactcagcg acttgcttcc caatgggcat cgtcggatct aaagaggtca
     2821 ttggatcttg gaaaatcatc gctatttctt taccacggat tttttgcatc tctctttctg
     2881 attttccaac gatatcgtta cctttaaaga ggatttgacc agcatctata ttggcgttgc
     2941 ttgacagcaa gcgcatgatg cttctggtcg tcactgattt tccacttcca gattccccaa
     3001 cgatcgccaa tgtttctcct ttattcaaat gaaaattgac accgcgaatc gctcgtacct
     3061 ttccagcata cgtatcaaaa gagatttcta agtctttcac ttctaaaatt ttcgtcatcg
     3121 tcttcactct ttcattttag gatcaaaggc atcacgcaat ccatcagcaa gtaagttaaa
     3181 gccaatcatg ataaccgata aagttactgc cggaatccat aatagataag gcagatacag
     3241 gaatgtcttg tacccttcgc tcaacatcgt tcctaatgaa gctgacggag gcgtaagacc
     3301 taaaccgata aagcttaaaa aggcttcgaa gaaaatagca gatggaatac taaacatcat
     3361 ttgaataata atgacactag agatattcgg caaaatatgt ttgaatgcaa ttttcattct
     3421 tgattcacct aatgtcaatc cagctaaaac atattcttga tcttttaatt tcattgtttg
     3481 tgcacgaacg atacgtgcca ttggtatcca gtttgtgatg gccatagcag ccacgattga
     3541 aaaaattcct ggttcaaaaa cgaccaacat caaaatcatg acaacgaggt ttggaattcc
     3601 tgataagatt tccaaaaaac gttgcatcac gttatcgaca cgtccgccta acaaaccaga
     3661 aaccaacccg taagctacac caatcgttac atctagtaat gcagcaataa atgcgatcag
     3721 caacgagatt ctcattccca taaataagcg gcttaataca tcacgtccca aaccgtctgt
     3781 tcctaaaaag aaatttacgt ttgaagggac gtttgcttga gcatatttat caacaagttc
     3841 accaccaacc attgcttttc cattcaatcc atcgatattg attcccggaa tacgaggagg
     3901 caaattgata taagccacgt tttgggcagt tggatcatga ggagaaacaa agattgtgat
     3961 cacagagatc aaaatgatga ccaatagtaa aaccattgaa ataacggcag ctttgttttt
     4021 ctttaaacgg cgccatgaat cttggataaa attcaaagaa ggtgcagtaa tctgttcacg
     4081 ttcttcttcg gtattttttt gtaatggaag aaattcatcc gcagggatat ctgcaactgt
     4141 ttcataacgc tttggtatca tttccattta tcctcggctc ccttctgaaa cacgaattct
     4201 tggatcaact agaccgtaca atagatccac gatcaaaata acaaaaatca gcataaatga
     4261 gtaaaggatc gttacagcca tgatcgtagg ataatcattt gttgtgattg acttaacgaa
     4321 ttgttccccg ataccaggaa tagcaaagat attttctacc accagtgatc cggtcatcaa
     4381 tgctacagct aacggtccta ataaagtcat caatggaatc aaactatttc gaagaccatg
     4441 tttaaaggcg atttcccaac ggctcaatcc ttttgctttt gctaattcca cataatcact
     4501 atgcagtact tccaccattt ccgtacggat aaaccgagca gagtcagcaa gcggcgacat
     4561 ggccaacgca attgttggta agattgtgta cacaaagcca tcccattttg cgattggcaa
     4621 aacttgtaat ttgattgcaa agatatattg taataaaaca gcaaatacaa agtttggaat
     4681 ggaacgacct aatatcgcta cgagtgtaga agatgtatct gcccatgtat tttgcttcat
     4741 ggcagagatc gttcctaaaa tcgtccctag gactgttcca aagataattg cctgtaaccc
     4801 taactgcaag gatggtccga ttcttccagc taacaaatgt gcgactggct ggtttttaaa
     4861 ttggaaggaa attccaaaat ctccttgaag caaattagaa agataaatcc catattgtac
     4921 gataactggt ttatccaaac cgacttgttt atttagcatc tcgattgttt caggactcaa
     4981 acgttcctga ttcgtatatg gcgtgcccgg cagcaattgc atcaagaaaa aggtgattgt
     5041 tgcaattaac caaagcgtga tgatcatgaa aaagacacgt tttaatacat atttgatata
     5101 actattcact aatgtttccc cctagctttt gacttctgac agatgtttcg ctacattatt
     5161 agataatgaa ccaaaaataa aggagcaatc aaatgaaaaa aaaatctatc ccctacgcag
     5221 tagcttttct actgattctg gttattttaa taaaaaatct catcaaccat tcctttacac
     5281 tcattcaatt atctaatgat ctattcttat ggtcattgcc gtttctgatt atcggcggtt
     5341 ttttatgggt cttttcttcg ggtttcttcg atcattttca gcgatcggtc catcttgcac
     5401 gaacaaggaa ccgaaagaaa aaaccagaat tttcctcgct ctcgtctgcc agctacggta
     5461 tgtacagctt ttggctgatc attgccggaa tactaattgc tctttctgct attttcatgc
     5521 ttttttcttt gttaggataa gtgaatatga gaacgaggtt gcgacatttg ccgcagcctc
     5581 gttttttctc atgctcacgg tattaacgat tattcttcaa cgtatgtcca tttgtaatcc
     5641 catgaagcgc ctgccgcatg gtgaacgata cctttgattt tttcattcac taggtgacct
     5701 tctgcttttt ggtacaccgg aacgacaccc atatcatcag agatgatctt gattgctttt
     5761 tggaaatcat cccaacgggc ttgttcatcg ccagcgtctg ttgatgaagc tgctttaact
     5821 gcttcgtcat attctgcatt gctccagcga ccacggttgt atgaattacc tgtgacaaac
     5881 aaatcagtaa agctgcttgg gtctgcatag tcagcagccc atccgcctag tactgcgtca
     5941 aaatcacccg aagttgagcg gtcaagacgt acagagaatg gaacaggtgt tggtttgact
     6001 gttacgccat ccaagttttc ttggattgag ttttgaacat attcgattac ttttttcgat
     6061 gaatcatcat cagaagccat caaatcaaat tctaaagaat caacgcccaa ttcttttttc
     6121 gctttttccc aatattcttt tgctttatct ttgtcataag aaactaattt tccagcttct
     6181 tttgcaaagt cttcgttcgt tgttggactt tttaccatat ctgatgggat caatcctgta
     6241 gagacaactg atccgtcacc tagtacttgt tttactaaag cttcacgatc gatagaataa
     6301 gaaatagctt ttcttaggtt cacattgttg aatggtgaat tcttttcacg ttgattgaat
     6361 tcgatatagc ttgtccgcgc ttctttaaca gaagtataag ctggatcgtt tgcgttttgc
     6421 tgtgctaact caccagacaa gatcacgtca tctgcttggc catctttgaa taagttcaat
     6481 gcagtagaag attcttttac tacactgact ttgatttcag aaagttttac tgtatctttg
     6541 tcccagtaat gatcattttt cttataagtc cattctgtat ctgttcctgg tccatcaaaa
     6601 tcttctaatg tgaatggtcc attataaaca gctttatcac ttgcagatgc ataggcatcc
     6661 ccattttctt ccactacgga ttgtttttgt gggaaaaatg atgggaaagc aagcagatat
     6721 tggaaatatg gtgtttgttt cgtcaatttg atttctaatt catagtcacc aactgctttg
     6781 atccctagtt ctgatttatc tttctttcca gctgtaatgt cttctgcatt ttcaactggc
     6841 gcatacaggt aagcatattc agaagctgta gctgggtcta ctgttcgttg ccagccataa
     6901 acgtaatccg ctgctgtaac aggatcacca tttgaccatt ttgcgtcttc tcgtaatttg
     6961 attttgtaag ttaatccgtc ttcactgact tctgccaact cgctagctcc tgccggctgt
     7021 ggtttgctgt cttcatcaag acggtaaatt ccttcataaa cgttattcaa tgctgtaaaa
     7081 ctgattgtat ctgttgcaac tgataagtca gctgtaggca tttcttgagg cacgactaag
     7141 ttaaatacgc ctcctccatc tgctgaaccg ctttttgctg aactactgct tgtggctgaa
     7201 ctgccgccat aacatccagc aagtacaagg ccgcataccg caaccaaacc aaatgtaaat
     7261 ttctttttca ttttacgtac ccctttattg gaattttcag aataattaat attttctgaa
     7321 gttgtttaag agtttaatga ataattatta aaaaatcaac actttttttt gtctttttca
     7381 taaaataatg attttttcgt gaggtttcaa ggaatttttc agaaaaaata gctttgtaca
     7441 aggttttcca tggtcttcca aaagccatta aataattttc gttatattgt ctattattgc
     7501 catacaaaga taaaaaagaa taaacaaaaa gaaactgtga caaaagtctt gtcacagtcc
     7561 cttatctgaa taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttat
//
LOCUS       DAZUKR010000123         7590 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000123 DAZUKR010000000
VERSION     DAZUKR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.125"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..787)
                     /locus_tag="ACG89C_002308"
     CDS             complement(59..787)
                     /locus_tag="ACG89C_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4900829.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="ACG89C_002309"
     CDS             complement(800..2080)
                     /locus_tag="ACG89C_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4900830.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="ACG89C_002310"
     CDS             complement(2221..2823)
                     /locus_tag="ACG89C_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4900831.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="ACG89C_002311"
     CDS             3068..3274
                     /locus_tag="ACG89C_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900832.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="ACG89C_002312"
     CDS             3586..4305
                     /locus_tag="ACG89C_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4900833.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="ACG89C_002313"
     CDS             complement(4386..5069)
                     /locus_tag="ACG89C_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS4900834.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="ACG89C_002314"
     CDS             5287..7473
                     /locus_tag="ACG89C_002314"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4900835.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2719 a         1280 c         1427 g         2164 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa
//
LOCUS       DAZUKR010000124         7107 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000124 DAZUKR010000000
VERSION     DAZUKR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.126"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..949
                     /gene="atpB"
                     /locus_tag="ACG89C_002315"
     CDS             230..949
                     /gene="atpB"
                     /locus_tag="ACG89C_002315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4900836.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            1002..1217
                     /gene="atpE"
                     /locus_tag="ACG89C_002316"
     CDS             1002..1217
                     /gene="atpE"
                     /locus_tag="ACG89C_002316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS4900837.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            1308..1832
                     /gene="atpF"
                     /locus_tag="ACG89C_002317"
     CDS             1308..1832
                     /gene="atpF"
                     /locus_tag="ACG89C_002317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4900838.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            1819..2361
                     /locus_tag="ACG89C_002318"
     CDS             1819..2361
                     /locus_tag="ACG89C_002318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4900839.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            2384..3940
                     /gene="atpA"
                     /locus_tag="ACG89C_002319"
     CDS             2384..3940
                     /gene="atpA"
                     /locus_tag="ACG89C_002319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4900840.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            3954..4856
                     /locus_tag="ACG89C_002320"
     CDS             3954..4856
                     /locus_tag="ACG89C_002320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4900841.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            4879..6285
                     /gene="atpD"
                     /locus_tag="ACG89C_002321"
     CDS             4879..6285
                     /gene="atpD"
                     /locus_tag="ACG89C_002321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4900842.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            6299..6721
                     /locus_tag="ACG89C_002322"
     CDS             6299..6721
                     /locus_tag="ACG89C_002322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4900843.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         2304 a         1276 c         1543 g         1984 t
ORIGIN      
        1 gataaaacat gattatcata ggttttttct tagatcagga gcaccgtgta taaagaaaaa
       61 ttcgcttcgt ttttgactga aataaaaaag tgtctaattt tcatgaaaat gttttgaatt
      121 ttctgtttca tggtggtatg ataccgtgtg gttagaaacc gattggaaaa aaccagatac
      181 gcacatgaat tgattttatg tgaatttcgg ggaaaagagg tgaaatggat tggatgaacg
      241 ctcgctaacg ttccacattg gaccaatttg gtttgatggt acagtttgta taatggtgtt
      301 actgacctgt ttgattgttt tcttcttagc ttactttttt acaagaaatc ttaagttgaa
      361 accaacaggc aaacaaaatg cgatagaatg ggtcattgat tttagtcgag gaatcgtaac
      421 agataatctg cctagaaaag aattgagcaa tttccatttg ttggcattta cattgttttt
      481 atttgttttt gttgcaaaca acatcggttt agtaacaaaa atcgttttac ctaacgaaac
      541 aacactttgg aaaagcccga cagcagaccc gtttgttact ttaactttgg catttataat
      601 gatcttactt actcacttat ttggggtgaa aaagttaggc ttcaaaggat atttcgtaaa
      661 ttcgttcttg aaaccatata gttttatgtt tccgatgaaa ctgattgaag aatttacaaa
      721 cttattaaca cttgctttac gtctttatgg gaatatttat gcgggggaag ttttgctaac
      781 cttgattgca aaaatggtaa taagcttagg ctggttctca ttgccgttag caattccatt
      841 agagatggtc tggatcgcat tctccttgtt tatcggaagc atccaagcat ttgtcttcgt
      901 tactttatct atggtttata tgagccataa gattgaagtg gaagaataat attcgtttta
      961 aaaacaaact actatttatc taatttagga ggaaaacaat tatgaattat atcgcagcag
     1021 caatcgcaat catgggagca gcaatcggag ccggttatgg taacggtcaa gttatctcta
     1081 aaacaatcga atcaatggct cgccaacctg aaatgtctgg tcaattccgt acaacaatgt
     1141 ttatcggggt agctctagtc gaagctgttc ctatcttagg ggtagttgtc gccttgattc
     1201 tagtttttgg tgtttaattg aatcagtatt aaggcagaaa caatttggtt tgttactgct
     1261 tttttacatg aaaactagaa gtacttcaaa gaaaggaagg ctcgcctatg ctgaatcaat
     1321 tggcaattgc agaagttggc aatgcgatgt taggaaatgt catcgttgtc agcggctcat
     1381 ttttgatact actggctctc ttgaagcatt ttgcttgggg accaatcagc gatattttga
     1441 aaaaacgtga agacaagatc gccaatgatt tggattctgc agaacaatct cgcatcaact
     1501 cagcgaaaat ggaacaagaa cgcgaacaac aattgttagc ctctcgttct gatgcagctg
     1561 atatcatcaa aaatgcgaaa gaaagtggag aattaagccg ccaaaatatt ttgaaggatg
     1621 ctcaagaaga agcagctcgt ctaaaaagta aagcccaagc tgatatcact gtagaacgtg
     1681 attcagcgct gaactctgta aaagacgatg ttgcagaact atctcttcaa atcgcggaaa
     1741 aaatcttgaa caaagaatta tctccagaaa tgcacgaatc attaatcaat caatatatcg
     1801 aaggtctagg ttcttcaaat gaaactagat aaatatacag taggaagacg ttacgggaaa
     1861 gctttatttg aactggcgat cgattcaaat aaagcagaag aaatttatca agatctcgta
     1921 agcttacgtc aaatctatga acaagtacct ggattaggta atatgttaag tgatgttcgt
     1981 ctagaacctc atgaaaaacg agaaatcatg gacaagcttg ttagtggcta tgatggaatc
     2041 gttaaaaact ttttagaagt tgtttatgcg tataaccgaa tggatgatct tttgttcatg
     2101 atcgaagaat atgaacgtcg atatgatgaa catcaaggtt tgctgttggg aagtgtcaca
     2161 acagcagttc cactttcaga cgaacaacga atacaattag aaaagaatgt tgcaaaaaca
     2221 atgaactatc aatcagtcga attaaaacaa atcgttgatt cttctattat tggtggcgcg
     2281 attgttgaag ctaaccatcg agtaatcgac ggaagcattc gcacgcaatt agaaaatatg
     2341 cgtaatcaat taaatagata gtacaaagag gtaggtgaat cgaatggcca tcaaagcaga
     2401 agaaattagt gccttgatta aagaacaaat tgaaaattat caaaacgtgc tttcagtcga
     2461 agaaatcggt accgtaactt atgtaggtga cgggatcgct cgtgcacatg gtttagaaaa
     2521 cgcgatgagt ggtgagttgc ttgaattttc aaatggctca tacggaatgg cgcaaaactt
     2581 agaatcaaat gatgtaggta tcattatcct tggcgatttc gagtccattc gtgaaggaga
     2641 taaagtaaaa cgaacaggaa agatcatgga agttccagtt ggagaggcct tgattggacg
     2701 ggtagttaat ccgctaggtc agccaattga cggactaggt gaaatcgtta ctgacaaagc
     2761 tcgtccagta gaagcaatgg caccaggcgt tatgcaacgt aaatctgtta acgaaccaat
     2821 gcaaacaggt ctaaaagcaa tcgacgctct tgtaccaatc ggacgcggcc aacgtgaatt
     2881 agttatcggt gaccgtaaaa cagggaaaac ttccattgcg atcgatacga tcatcaacca
     2941 aaaaggtcaa gatatgatct gtatctatgt agcaatcgga caaaaagatt ctacagttcg
     3001 tacacaagtt gaaacattga aaaaatatgg cgcaatggat tacacaatcg ttgtgaatgc
     3061 cggtgcgtct caaccagcgc cgttgcttta tatcgcacca tatgctggta ctgcaatggg
     3121 tgaagagttc atgtacaacg ggaaacatgt cttgatcatt tttgatgatc tttcaaaaca
     3181 agctgttgct tatcgtgaac tttccttgct gcttcgccgt ccaccaggtc gtgaagctta
     3241 tccaggggat gttttctact tacactcacg cttattggaa cgtgcagcaa aattaagtga
     3301 tgatttaggt ggcggttcaa tgactgcctt accatttgtt gaaacacaag ctggagatat
     3361 ctctgcttat atcccaacaa acgttatttc gatcactgat ggacaaatct tcttagaaag
     3421 cgacttgttc tacgcaggta ctcgtccagc ggttgatgcc ggactatctg tatctcgtgt
     3481 agggggttct gcgcaaatca aagcaatgaa aaaagttgct gggactttac gtctagactt
     3541 agcaagttac cgtgaattag aagcatttac acaattcggt tctgacttgg atgcagcaac
     3601 acaagcaaaa ctaaatcgtg gacgccgtac agtagaaatc ttgaaacaaa aattgcatgc
     3661 accgctagct gttgaaaaac aagtagtgat tttgtatgct ttgacacatg gattcttaga
     3721 tagtatccct gtagacagta ttttagactt cgaacatgaa ttattcgaat atctggatac
     3781 caatcattca gatatctttg aaacaatccg tacaacaaaa gatcttccag aagaagaagc
     3841 gttgaacagt gcgattcagg aatataaaga catgttctta gctacaaaag gaaataattc
     3901 ctctacagaa gataaattga aatccattca aaatgcatag tgaggtgaga tgaatgggtg
     3961 cttcattaaa tgaaatcaag acacgtatcg cttcaacgaa aaaaacgagc caaatcacgc
     4021 gggcaatgca gatggtgtct gcttctaagt tgacaaaatc tgaagcttct tcacagaagt
     4081 ttcaggttta tgcaacaaaa gtaagagaaa tcgttacgca tttgactgct actcagttaa
     4141 atgatattgc ctcagaaaat cctagagggg atatcaatta caacagcatg ttgctcagcc
     4201 gtcctgttaa gaaaacgggt tatatcgtca tcactgcaga tggcggttta gtcggtggat
     4261 ataacagctc gatattgaaa caaacgatgt ctattttaga agaagaccac caatcacccg
     4321 atgattacgt gatgatcgca attggcggaa ctggtgcaga cttctttaaa gcacgcggta
     4381 tcaatctagc atatgagctg cgcaatctat ctgatcagcc aagctttgat gaagtccgca
     4441 aaatcgtaaa tatggcaaca actatgtacc aaaacgaagt attcgatgaa ttatacgtat
     4501 gttataacca tcatatcaat tcgctaacta gtcagtttcg tgtagaaaaa atgctgccaa
     4561 tctcagactt agatccagaa gaagcaacta ctttcgaaca agaatatatt tttgaacctt
     4621 caaaagaaga aatcttgtct cagttattgc ctcagtatgc tgaaagtttg atctacggtg
     4681 cgatcgttga cgccaaaaca gcagaacatg ctgctggtat gacagcaatg aaaacagcaa
     4741 ctgataatgc tgctaatatc attgatgagc tgacggtttc ttacaaccgt gcaagacaag
     4801 gggctattac ccaagaaatt accgaaattg ttgccggagc ttcggcacta gaatagtatt
     4861 gagattggag gaaaaaagat gagttcaggc aagattgttc aagtaatcgg tcccgttgtc
     4921 gacgtggaat tttctttaga ccaatcctta ccagatatca acaacgcatt agttgtttat
     4981 aaaaatgacg aaaataaatc aaaagttgtt cttgaagcag cgttagaatt aggggacgga
     5041 gtgatccgga ccattgcgat ggaatcaaca gatggtttac aaagaggaat ggaagtcatt
     5101 gatacaggca aagcaatctc tgttcctgta ggtaaagaaa cattaggtcg cgtattcaac
     5161 gtactaggag atacgatcga tttagaaaca cctttcccag aagatgcgga aagaagcgaa
     5221 attcataaaa aagcaccagc ctttgacgaa ttaagtacaa gtacagaaat tttggaaaca
     5281 gggatcaaag ttatcgattt gcttgcccca tatttaaaag gtgggaaagt cggactattc
     5341 ggtggtgccg gtgttggtaa aaccgtactg atccaagaac tgatccataa tatcgcccaa
     5401 gaacatggtg gtatttctgt atttaccggt gtaggtgaac gtactcgtga aggtaatgac
     5461 ttgtattatg aaatgaaaga ttcaggagtt atcgaaaaaa cagccatggt gttcggacaa
     5521 atgaacgaac caccaggtgc acgtatgcgt gttgctttga ctggtttgac gattgcggaa
     5581 tatttccgtg atgtagaagg tcaagatgta ctgttgttta tcgacaacat tttccgtttc
     5641 actcaagctg gatctgaagt atcagccttg ttaggacgga tgccttctgc ggttggttat
     5701 caaccaacat tggcaacaga aatgggtcaa ttgcaagaac gtatcacatc tacgaaaaaa
     5761 ggttcgatca catcgatcca agcaatctat gtgccagccg atgactacac tgacccggcg
     5821 ccggcaacag cattcgccca tttggatgcg acaaccaact tggaacgtaa attaacagaa
     5881 caagggattt atccagcggt agatccattg gcttcttctt caagtgcttt agcacctgaa
     5941 atcgtgggag aagaacatta taaagtagca acagaagtcc aacacgtatt gcaacgatat
     6001 cgtgaattac aagatatcat tgctatctta ggtatggatg aattatccga tcaagaaaaa
     6061 gtgttagttt ctcgtgcccg cagagtccaa ttcttcttgt cacaaaactt taacgtggct
     6121 gaacaattta ctggtcttcc aggttcatat gtccctgtag aagaaacagt gaaaggattc
     6181 cgcgaaatct tggaaggcaa atacgatgat cttcctgaag aagctttccg aagtgttggc
     6241 cgaatcgaag acgtcgtaga aaaagcgaaa acactaggct actagaaagg ggtgccccat
     6301 ggatcaatat ttaacagtaa atgtggtaac tcctgccggt ttggtctatg atcaccatgc
     6361 agctatcgtc gttgcacgta cgacagctgg tgagcttggg attttgccta aacatgcacc
     6421 aatcatcgtt ccattgacga ttgatgaagt tcgtgtaaaa cgaacagatt cagataccca
     6481 cgtcgattgg atcgctgtta atggcggaat catggaagtt cgtgataatg ttgtttcaat
     6541 cgttgctgat agtgcggaac gtgaacgtga tatcgatgta tctcgtgcgg aacgtgcaaa
     6601 acaacgcgca gaacgccaaa tcgaagaagc aaaagcaaaa caagatacga acgaattgaa
     6661 acgggcaact gttgcgttgc accgagcaat caatcgtatc aaagtatcta agcactcata
     6721 aaatgagatg cggatgttta tccgtgttta agaacagtaa aaagaggtta tgacagaagt
     6781 ggtcagcttc aagacataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
     6841 ctcagcttat ttttcgaaga agctacttct ggaacaccgt atatcggagg ttgtgaaatc
     6901 agcgttttgt cctgagtatt agagaaacaa atggaaaata gttcctcata ttttgccgtt
     6961 ttttgagcta ataccgaggg tcagatgatt gaacaccgtt tatttggaat aagggactgc
     7021 gacaagactt ttgggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctga
     7081 gtcagaatga accacattct ggctcag
//
LOCUS       DAZUKR010000125         6916 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000125 DAZUKR010000000
VERSION     DAZUKR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.127"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1623)
                     /locus_tag="ACG89C_002323"
                     /pseudo
     CDS             complement(1..1623)
                     /locus_tag="ACG89C_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2062..2931)
                     /gene="scrK"
                     /locus_tag="ACG89C_002324"
     CDS             complement(2062..2931)
                     /gene="scrK"
                     /locus_tag="ACG89C_002324"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4900844.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2951..4072)
                     /locus_tag="ACG89C_002325"
     CDS             complement(2951..4072)
                     /locus_tag="ACG89C_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4900845.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4074..4469)
                     /locus_tag="ACG89C_002326"
     CDS             complement(4074..4469)
                     /locus_tag="ACG89C_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4900846.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4488..5285)
                     /locus_tag="ACG89C_002327"
     CDS             complement(4488..5285)
                     /locus_tag="ACG89C_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4900847.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5287..6075)
                     /locus_tag="ACG89C_002328"
     CDS             complement(5287..6075)
                     /locus_tag="ACG89C_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4900848.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6089..6565)
                     /locus_tag="ACG89C_002329"
     CDS             complement(6089..6565)
                     /locus_tag="ACG89C_002329"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4900849.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6637..>6916)
                     /locus_tag="ACG89C_002330"
     CDS             complement(6637..>6916)
                     /locus_tag="ACG89C_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4900850.1"
                     /translation="RVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLED
                     EIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         2227 a         1434 c         1039 g         2216 t
ORIGIN      
        1 tttatatgtt gtaacttcct gataagaaaa ctaattaaaa gaaagaggta actacataaa
       61 ctaattaaaa aagctaactt catagttgga ggataaatgg aagaaacttc aatgattgtt
      121 tctccttgtg ttccttcaat cgacactagt cctattatca atctctcttt tttgtccatt
      181 tacttttgcg actgttcccc aataatcaaa tacaggaagc gttatttttt gtcctttaga
      241 ttcatgtact gtatagacta ttttcgaatc catcacttga taaggcatag tatgccattc
      301 cttgccaatt cttacttcat gattctgcaa atgttctttg tattgtaatg cttccgtggg
      361 tgcatagtca taaagtcctc cgggaaggag tgtcgtgtaa cgaataagat tttcttgatt
      421 cattaccaag tgatctctct gagtgacaac tttatgttca tagtttgctt ttgttgcagc
      481 agatacagcc aacagtaata aaacaattgc cgttatatta actacttttc tttgcttagc
      541 tgatgtgctc ttcatgaaaa cgaacgataa aatcaacgag ccaaacagta cttgaaagcc
      601 taagattcgc caaggaaatt gaatcatttg aatgggtgta ttttgtaata aagaccatgg
      661 aaaaaatttg gtggataaga gccaagagac taccgcacta aggtaaatca ctttttcttt
      721 gcgattaaat cctttaaaaa agaagagtgg acagatcaaa gaaataatac caatgattcc
      781 taccgtaaag gttgtcaggt cattattcat cgcaccaatc aaactatccg agacatttaa
      841 tgcttggtgt tgtaaggtcg ttttatctgg ctcattaatc gattgaacca tgatttgtcg
      901 gaacatcgga taccaaaagt atgctgttaa aagaatcgtc attcctactg cctttgacaa
      961 ctccacaatc aaccggatgc tttctttttt tcttgtttca cttactacaa agccgcctaa
     1021 gactagtaag ccaacggcta ttacactcat aaaagtggaa agaacatgtg tataaatgag
     1081 taagctcata ctgactgcta atggaattgc acatggcttt ttctcataaa tcagtgcgta
     1141 agcataatac ataactatcg gtataaccgt aagtgcaatc gcttcagcaa tagcagaacg
     1201 atgataaata tcaatcgtac ggtacccata aaatgtatat aaacagctaa atataaaagc
     1261 cgcatcgatt ctttttaaaa attttagtcc ataatgaaac atcagcatga tagtacaaac
     1321 attcaatagc caaacatata agatataact aacgattaaa ttattactta tccaataaaa
     1381 aatgactgcc ggaaaaaagg tcaagtaggg ataaaaataa ttcactccgc tcccataatg
     1441 attaaacgtc gtaaaattga ttggaccaga aaaaattgaa gataaccctt tgactctagc
     1501 aaaatggaag gcaatatccc ccgatgtaga aatagtatga acatttctaa aataattgat
     1561 tgaataaagt ccagcaataa ttgctaggag aatggacaat attatttttt tctttacttg
     1621 cattacttaa ttatatactc ctttcatgga ttgctttatc tagtataact taaattttaa
     1681 ataaatcgtt atttattttt actctcactt aaaaaagcac taaaattata agagagggag
     1741 gtaatggtct gaacaccaag aattgttcca acatcgaatt gcttaactaa tgagttcaaa
     1801 gcttaattat gtggctctta tatttaccga gcgtggattg cgatctcttt agcgaaacaa
     1861 acatctttca taaaaaaaac agccatatcc gctttcttgg atatgactat ttactactac
     1921 tttactatct ttcattcttc ttgtctcact tatgataagg cgctgggtat catgagtgaa
     1981 tgatattgct gaatacaaat agtccgacca cattaagttg atcggacttc ataagcattt
     2041 tattgactaa tacttttttg cttaagaatt gattctaaca tcttttgcta aagttaagca
     2101 tcctatagtt cctgcattat ctgctaactt aggtgtgaca atgtattctt cgatatttgg
     2161 cgtatcgaca tagccgttaa ttagttctaa aaaacgtctt tgtactttct ttttcagatg
     2221 ttcttgttgc atgacaccac caccaaaaat tataatatct ggtgaaagca tcagggtcgt
     2281 attacaggca cattgtgcaa tataatccgc ctcgatttcc cagaacggat cttctataga
     2341 aagatcttgt cccttggttc ccgtacgcat ctcgatagcg ggtccagatg ccaacccttc
     2401 gagacaatca tgatgaaaag gacaagtacc agagaaagta tctcctgaat gtctcttaac
     2461 taacgtatgc cccatttctg gatggctaaa cccttcaaca aaaatcccat cttgtatagc
     2521 tcctccacca attccagttc caacagtgta ataaacaaca ctggataaac cttttccatg
     2581 acctgaaaca tactccccgt aacaagcagc attcacatcg gttgtccagg caattggaat
     2641 ttcgaaatgc ttattcaacg ttccaacaaa atcaaaattt ttccaggcag tttttggagt
     2701 agaggtgata tgtccatagg tctttgattc acgatggata tcaatcggtc caaatgaccc
     2761 tactccaatg ctttctaatt gttctttata gtgattaaag aaatcaatta ccaaactcat
     2821 tgtttcattc ggtgtagttg taggaaaact cactctttct aaaaccttta aatcatcatt
     2881 gccaatggca caaacaaatt tagtcccacc tgcttctatt gagccataca tttcttactc
     2941 caccgtcctt ttatctagga attcgttctt ccctgtctaa tagtttttcg aaaaactcat
     3001 gtggttcctt ttggtaccaa taggcaacag aagaaatatc gtcttgacgc tcataaagct
     3061 tgatgtcatc atttcctagg gtttggaaag taacacgtaa attttctttg aaactaatgg
     3121 ggtcgggtaa atgccagcgg tacaacccat gcatcggtaa agcatcatcg ccaaaaccat
     3181 gaactggatt tggattaggt tttccagcac taaaataatc ccgggtgtga tctcttttag
     3241 attgaaaagg atagcccata aatagcgtat taaaattttg agcatatgcc ctttctccaa
     3301 agttcttttt atggaaagcc caagcgccgc caaaataatc ttctgctcct gtagatgtta
     3361 ttgtagggaa atcttcatct ccatctatat aaaacttaaa ttctccttcc ccccaccagt
     3421 atctttccaa tgcgcacaaa gcaaagtagg ttcctacata atacccagtg cctttgattc
     3481 catcaataag ggtgtaatct tttccttttg tcgtgatggg ttcacggttc catttagcat
     3541 gaaaatacat cgcatcatcc ggtacttcag tcaatgcata attaaatgta taaaaaatgt
     3601 gcttaataaa ctcaggatgt tcattcgtta aagtaatctt agcttttttt cgaaatggca
     3661 tctcaaaata gcaattcatc cctgatgttg gattcactac gattggtaag gaattcactt
     3721 cgcatcttgt cccaaaccca ttacaaaaaa aatctcctaa tggactttct acagaaggag
     3781 tggattcatc atcccaataa atacgcaaaa ttaaatcacg taagacgaaa ctcccagcga
     3841 ttgttttgtc tgtcactgtc atccaaaaat gacgtaaaat tccagggcct tccatttctg
     3901 ctaaagtaat tgtctctcca gcatttatcg gaacagaagg tgaccctttt ctcccaattc
     3961 ctagatgact cgctgtcatt gccgctttcc ctttttcacc atttttattt tctggtgaaa
     4021 tagttctact ttcctctccc ttgtggagcc atatattatt taaacaattc attttattcg
     4081 tcctctatcc tttcttcact tttttgatta ttcatataga tgatttgttg ttgtgcctcc
     4141 gcaataattg ctccaatatc taaattatca tttcctaact gcaaagcgat tgacattgcc
     4201 aagggaagat tcattcctgc aattacgtgc actctgtcag aaatataggt aaaaaacttt
     4261 tgatttacac ttcctccagc catgtcagtt aaaacaatta tttcgtctgt agtatccact
     4321 aactctttaa tttgttcatc tatatcatca atttcttcga catatgcaca taagctacta
     4381 atttgattat gcatacctgt taaaaactct agtgtctctt taaaaccctc agctagtatt
     4441 gaatggctag caatgatcaa tcttctcata attcaaatcc ttttccttta cgctaatatt
     4501 ccaaatgcag ccataaccat cgcaaaaaca ataacgagta gaatcaaaat ggtcatactg
     4561 atttttttct tactgagtag ataatatgct gcagcagtaa taattactgg aattaatgtt
     4621 ggtaaaattt tgtccaacat tggttggatt ttcatactaa cttcaccaaa agtgaattta
     4681 agtggcgttg aaacactgat aactgaagca attaatgatc caactactgt tagccccaat
     4741 actgaagcag cttcagttaa ataattaatc ttttcaccgt attcagtaac aatcttcatt
     4801 ccctgcttat aaccaaattc aaagcctctc attcggaaaa agaagattac catgattaaa
     4861 atgaaccata aaaagattcc tacaggatta ttttctaaag ccatataagc cgcaattgat
     4921 cccatgatgg ttgggatcat cgtcatcaaa agagagtccc caataccagc aattggaccc
     4981 atcgtaccaa ctttaaagtc attaatcgca tcttttgctt ctaatccttg cttttcctcc
     5041 attgcaacac aagcacctaa aataatggct gccagccaag gctgtgtatt gtaatactga
     5101 aagtgattat ttaaagcttc gatgtattca tcatcatctt tgtataattt tcttaaaata
     5161 ggtgacaatg cgtaagtcac agatgcacct tgctgtgttt gataattaaa ggtacaaact
     5221 gacatcaacc aacgatagtt cgctttccta agatcctttt tcgttacttg atatttttcg
     5281 ctcattttat tcatcctccc caataaaccc aacatcagaa gctcccgcag aagcagtgat
     5341 ttgtgcatct ttttcagctc tatgatacac attaaatgct aaagcgaatc ctatgatcgc
     5401 cgctccaata ataggtacct tcaaaaaagc ggctaagaaa aatccaacaa gtaaataact
     5461 aatgtatcga cctgtaggca taaagcgtaa caacatcgca ataccaacag caggcaacat
     5521 ttttccggct aatgttagcc catccataaa ccaattaggt aaaacatcca aaacgtaatt
     5581 aacagcagac tgtcctaaag taacggcaat aaatactgga attgctgcag ataaaccaaa
     5641 gaaaattggc cccaaccaca aaacactatt cattttctta aattctcgtt tattcgcata
     5701 ttcctgtgat ttgcgaacaa caaaaccatt taagattttg acaattacat ctagttgcac
     5761 ccctaacata cctacaggta gtcctattgc taatccggct tctactccaa gctggctacc
     5821 aaatgccgtt gcaataattg cgcacgttcc atagtccggt acagatgagc caccaaaacc
     5881 ggcaactcct aatgacatca attgaactgt accgccaact atcaaacctg tttttatatc
     5941 acccatcaaa attcctgtga ataatcccca aaaaactgcg taatttacaa agatcatcgg
     6001 aatcccataa taatcaacat gtttaataaa tgctaatata gtcagcataa taatttgaaa
     6061 aatagtaaaa tgcattatcc ttactccttt atcctaattt tgattgaatt tcctgtgcct
     6121 tatccgccgg aacaaattgt gtaacaattt ctacaccaga atccaacaat tttttatacg
     6181 tgctgatatt ttcatctgat gcataggctc tagaagatag ctgtaatgca ttatctttaa
     6241 caacagtacc gccaacaatt agtcggtcaa ttctaatacc gttatctact aaatgaagga
     6301 ttgtttccgg ctctttcacg atgacaaata ctttttcctg atcgtatttc ccctttttaa
     6361 aattcgcaat cgctgtttct tcagtgataa ttgatacagc cattccagca ggtctagcta
     6421 aacgcatact tgatttttgg atgtcgttat ttgcgacaga atcatcgatc accatcactc
     6481 tgtttgcttt agacattggt gcccattgcg tgacaataat cccatgcaat aaacggttat
     6541 caactctcga cataacaata ctcataaata cttcctcctt tatctttcat tttcttacaa
     6601 gcaattacca tgccaaaaca aaaagaactt gataaatcaa gctcttttta cataatcata
     6661 aacgtattta acctcatgaa tcgggatgtc gacactataa taatgttcaa cgacactgaa
     6721 agattgctta aaatgtgtct gaaatttttg aaattcctca ctcgtttcat tgaaataaat
     6781 ttcatcttcc aaagtatttt gttttactaa tcgttcaatt aagcaactaa tgtgaacata
     6841 taatccgaaa cacgtattat tatcaaatga aattcctaga tcattttgta agatgtatat
     6901 agcatctgaa acccgt
//
LOCUS       DAZUKR010000126         6785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000126 DAZUKR010000000
VERSION     DAZUKR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.128"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1030
                     /locus_tag="ACG89C_002331"
     CDS             20..1030
                     /locus_tag="ACG89C_002331"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4900851.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1099..2043)
                     /locus_tag="ACG89C_002332"
     CDS             complement(1099..2043)
                     /locus_tag="ACG89C_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4900852.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2027..4747)
                     /locus_tag="ACG89C_002333"
     CDS             complement(2027..4747)
                     /locus_tag="ACG89C_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4900853.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4744..5964)
                     /locus_tag="ACG89C_002334"
     CDS             complement(4744..5964)
                     /locus_tag="ACG89C_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4900854.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6245..>6785)
                     /locus_tag="ACG89C_002335"
     CDS             complement(6245..>6785)
                     /locus_tag="ACG89C_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFS4900855.1"
                     /translation="YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICS
                     IPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQ
                     NVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYV
                     TVSNDKDGVSQAFERILTI"
BASE COUNT         1993 a         1296 c         1098 g         2398 t
ORIGIN      
        1 tacaggagtg caaattttaa tgaaaaaaaa atcaattatt ttggcagcta ctagtgcgtt
       61 ggctgtttta actctagcag cttgttcagg cgatactaat aaagatatcg caacgatgaa
      121 aggcggaacg atcactgtat ctgatttcta tgatgaagca aaacttgaat catcgaacca
      181 atccttagta caacggatga tcatttataa agtattcaat aacaaatatg gcgataaagt
      241 aaccgataag caagtggatg cagaatacga caaacaagcg aaatcattag gtgatacttt
      301 tgaatcacaa ttagaagctg ctggatatac gaaagatact tataaagaat atattcgtaa
      361 caatttagct tttgaagctg gcttgaaagc tcacgtagat attacagatg acgatttgaa
      421 aactgcttgg aaatctttcc atccagaagt agaagcacaa atcatcaaat tatcaagtga
      481 agatgaagca aaagatgtga aaaaatcagc ggatgatgga gatgatttct ctaaattagc
      541 caaagacaaa tcaactgata cagagacaaa agaagatggc ggtaaagtta aattcgactc
      601 aacgacaaca actattcctg cagaagtaaa agaagcagct ttcaaattga aagacggtga
      661 gatctccgat gttattacta caacaaatcc tacttcttat gcgacagaat actacgttgt
      721 caaaatggtg aaaaaccaaa ataaaggcaa tgacatggat aaatacaaag atcaactaaa
      781 agatatcgct actgaaacta aactaagtga caatgcattc acaactaaag taatcggtga
      841 agaattaaaa gatgcaaatg taaaaatcaa agatgatgcc tttgaaaatg tgttgagtgc
      901 attcacaaca acttcatcaa gtacgaaaga ctcaagtgaa actacagctt caacaaagag
      961 ttctgacaca aaatcaactg attcaacaaa agaatcttca actgaagaaa caactgattc
     1021 atcaaaataa aagaaaacaa aaaaagcgaa gacttatgtc tcgctttttt tgttttgaat
     1081 gaaaaaaata aatttttgct ataaatcttt tggtacatag aaacttcggt tgtccatacc
     1141 gaaaatccga tcggtgtatt ttcctggttc tgtttgacga atcgaagtca acatcatctg
     1201 catcgaagca tcgatattgt cgatttgatg taatatttct gcttccatta ttcttggacg
     1261 tacaggtgag ccatattcta ataagccatg gtgtgctaaa accatatgac gtaatacaag
     1321 aacatcttcg tcagcttcgc tgattttcaa cgcaagacag gctttagtta tttcttcgtc
     1381 aatcaatacc agatgaccga tcaaatttcc tgcaagcgta tattctgtcg acataggacc
     1441 ggaaagttca atcacctttc ctaaatcatg caaaatgata ccagcataca acaatgctcg
     1501 attcagctca ggatattcat cggcgattga tttccctaag tgaagcattg tcaaagtatg
     1561 ataagctaat ccactggcaa atgcatgatg gtttcttttt gctgccggaa actcaaaaaa
     1621 ttgtttttga tatttattaa gtagatagcg aacaatacga ttccaatgcg cattggtgat
     1681 ctcaaacaac aattgattga tttcttcttc catttcttct ttcttcaaag gggctctttc
     1741 catgtataaa ctaggctctc ctggctcatc tacccttgtt aagcgcatat gaagaatctt
     1801 tacttgaggg tttccttgat aaacctctct tttaccgttt agaaaaacga ctgtgccggc
     1861 tgtaaattta ttgatttcct cctccgaagc atcccagtat ttaccatcga tagtacctga
     1921 agtatcttgg aaagtaaaag caataaattt ttttccgttt ttagcgattc ttacatctgc
     1981 acttttgatc aggacaaatt gttcaaactg ctcatcgatt actaaatcac gaattttttt
     2041 cattatttgc tccttctaat tgttttacga tttggttcat ctcatggtaa tagcttacca
     2101 tttctttatc agaagaaaaa caaattattt gatacttttc tccaaattta gctaaaagtt
     2161 cagctaattg atactttcgc tgatgatcat aatgaagcca gccatcatca atgatgatcg
     2221 ggcatatcga acggttagct tctaatgaga ggtaagcaaa tctcatcgcc ataatcagct
     2281 gatctttcgt acctgtagat aactcataga tgggaaattc tgtatatccg tctgttaaag
     2341 tcagtgtatc atttttcagt aatatcttct gataatttcc ttttgttaaa atacctaaat
     2401 aatcacctgc tgctcttaac aactggggta attgtctttc actcatctct gttgaaagat
     2461 cacctaagaa tgtcccggcg atttcaagtg ctccccattc ctccagtaat tcctgtaatt
     2521 ttgctttttg ccggctagct tgttgataaa gttcgtccaa tgtgccgtct ctttgcaact
     2581 cttctacttg aagttggagt cgttgccgat tttcacttgc ttctttttct tcttgctgga
     2641 gttgggattg cttttctttc agttggcgca gtcttacaga taatttctct tttgtcagct
     2701 cttcaggaaa tagtggagta agcatttccg ataattcatt gtatcttttc atctgctgtt
     2761 cggcttgttt ggctttttgt aaaaagacag gtacttccga aggataagat aagcctgctt
     2821 gagtcaattg ttcgctgtat tcttgtatcc atactttttg tttctttttc aattgattga
     2881 tttcttgctt caataaagta ttttgctgat agcttctggc aaatttcatc tgttccatct
     2941 cttgtataaa acgggaaagc aataccattt tttctgaaat tgtcttatct tgtatcggta
     3001 accattcttt aacaaactca aatttttggt cgatttgtcg tagtttttct tgtatttcct
     3061 gttctctttt atgtttccgc tcaaatacgc catataattt ctgataatct atgacttcct
     3121 gtccatattc tttcatgagt ttgagcgtat ccatctttcc taagtgccga tgatccgcaa
     3181 aacgatatac ttcttgctga agaatctgtt tttcttctcc agttctcttt tgtttttctt
     3241 ctgcttcagc taattgagct aaggctagat caagttgtcc tagtttttct tgccacattc
     3301 cttttatctc agagaaatca tttttagttt taatttttgg ttgccttttg tagaagaaat
     3361 agccaatcat gcctgcacca attagtaaaa ggatcacttt caagggcatt ggtacaaata
     3421 tacctgcaat gattattgct ccgccagcca tcagcatata cggtttagat gaagagactg
     3481 gtgctatctc tgactgattt tgtaataatg ctggataggt ctcttccaag tcattcactt
     3541 ctgcttcaat catctcttgc tgttctttag ctatttggag acgcacagaa atctggcttt
     3601 ctagatcagc aatccttctt tcttcagcca gaagatgctt ccattcttct tcatataaaa
     3661 attcttccgg taacggattt ttctgccaat gccacctttg ttccaaaatc ttcaattctg
     3721 aatttgtttc tctagtctca atttgtaatc tttgccattc ttcgtttaat tgttccgctg
     3781 taaattgaag ttgctggaga tcttttagtt cctgctcctg ctctaaataa aaataatatc
     3841 tggctgactg ctgatccatc ccgctatgct tttccagtgt tgcttctaat tcttccaaac
     3901 gctcatttgt ttgctgatac cgtttggaga aagactccag atctaaaaga agttgctcat
     3961 caggaagacc tgtagtgctg ttttttttga gagattgcca ttcttcatat aaagaaaaat
     4021 taagttcttg ctgtctggct aatgaaaatt gtttgttcgc ctcttttaat tgtgccaata
     4081 actgctgctg ttgcgactcg tattcctgtt cttgttggat caacttgcca aaataagctt
     4141 cctgttcttg tttttgatga attttttctt gcagctcttt ccattcattt aatttttgat
     4201 tgattagtaa tttttggcct tttttcttga ataattgttg tgctttctga taatattctt
     4261 gccgcttttg gaatacttga gaactgcccg tgatgcctaa agaaatcagc gcatcatgta
     4321 gctctttttc tctcaaatga tctagttcca tcaactgctc ttgctggaaa gtaaatacct
     4381 gctggaataa ctctttcgtc aaaggtgcga tcagtttttc tagtaactca tcacttccca
     4441 cttgatcccc cagccacacc ttcgattttc cgcgattttg ctgcttgtag cgctcgacag
     4501 caaattcacc atatacagga tgtttaagcc atattttgcc gccataggct gagccgtctt
     4561 ttggtgtata atcctttttt ttacggcctt ttgaagggaa accaaataat attgcttgaa
     4621 taaattgata aatcgttgat tttcctgctt cgtttccccc atagatcagc tgattgcctg
     4681 aataaaagtc aaaacttttt tgacgccatt ttccaaaagc agcaatctcg atccttaaaa
     4741 tcttcattcc tgatcctcct caaataaaaa atcttgatta agcagctgct tggctttttc
     4801 aagtgtttct tcttgatatt ccggcagttc gctcaatatt ctagccgctt cttgatgact
     4861 atatacttca tttaaaattt gttggaaatc ttgaggtgtc tgatagtatt gaaataactg
     4921 ttccatcaat ttagcagaag cagcaagtgg ggtcttcgta taaaaagtat tctctatgag
     4981 agaaatacgc caaataaaga aatcatttat cctatctgag aattcctcta acaaatagtc
     5041 taatagctct cctgattgta tccgttccaa aacatctgta ttgagatggt cataatcttt
     5101 taattttatc tcaattaaac taaatccatc ctctactttt gaaagctgct gacgaatgtg
     5161 aacaatgacc tcttttgtcg ttcttgcttt cctcaatgat atttcctttg tcttccaata
     5221 gacctctgct actttaatcg gatgtatttg gcaacttcct ttacttaatt caactagtag
     5281 aatagatgta gaagcttgtt cctttttagt atgaccttga ggcgcacctg gataaacaat
     5341 tgtttgcttt tcatttaaga ccgttggcac atggatatgc cctaaagccc aataatcgta
     5401 tcccttttct tgcatctttg agggttggaa aggagcatag tttccttttt gagagacgcc
     5461 aggttccccg tggtacaacc caatatggta atctgtgagt tctctagaag gaaattccat
     5521 cactttgtct ttttgaatcc acggttgacg ataactaaat ccactgagag agacagtttc
     5581 tccacttttt atcgttattt tttttgtctc taccgtttcg gatgtaaata aatggacatt
     5641 tttaggaaag gagaaccaat accgctcttt ttgatagaaa tcatgatttc cgaatattat
     5701 aaatacaggt atatggttct tttctaaccg tttcatctgt tctgaaaaat gtttctgtat
     5761 ttttaatgaa ggacgatttt gatgaaaatt atctcctgca agtaaaacaa aatcaacgtt
     5821 atttattatt gcctgttcaa tgatatttga taaaacggtc agattcgtct tcaatagttt
     5881 ttcctgtaca gttttatcaa gagttgtcag tccttcaaat gaccgatcca tatgtaaatc
     5941 tgccgcatgg ataaatttca ccattaaaaa tttcctcctt tttttctttt atcataccat
     6001 tttcaatgtt ttttttcatc aataaccgaa aatttgttcg tattatgcaa agaaattacg
     6061 ataaatggcc gacttcaaaa aataagttta ttcgaaaaaa agattgtgac atgatttttg
     6121 tcacaatcct aaatatctat gttatattcg cttcgtttga cactgaagaa gtactatttg
     6181 tactggaatt tatgctccat ttttccgtat ttagaattct atgttctata ctaccttaat
     6241 tagtttatat ggtaagaatt ctttcaaatg cttgagatac tccgtcttta tcatttgaaa
     6301 cagtgacata atcagctttt ttcatgattt cttgatgact atttcccatt gcaaaactaa
     6361 tatctgcact ttcaaacata gataagtcat ttaaactatc tccgatcgcg attctcaaag
     6421 gtttctttaa attgtagatt gattccaaac gatcaaacac cacaccttta gatacatttt
     6481 gaggtacgat ttcaatattt acaggtgccg attcagtaac agacacattt agcatctttt
     6541 tcaaatggtt aaatatatta gctttcaatt gacgattagg aataaaaata aaaaatttgt
     6601 aaatgctgat agcatccttt tctataattt cctcaatatt attataaggg attgaacaaa
     6661 tagatttttc atactcaagc tcatagttta gctctttgat agaaaattca ctccctacct
     6721 cgtgaaaaat ttttttcatt ttatttttat aattctgaga ttcataactt ccgagatttg
     6781 tatat
//
LOCUS       DAZUKR010000127         6776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000127 DAZUKR010000000
VERSION     DAZUKR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.129"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG89C_002336"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG89C_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4900856.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG89C_002337"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG89C_002337"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4900857.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACG89C_002338"
     CDS             complement(2303..2965)
                     /locus_tag="ACG89C_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4900858.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACG89C_002339"
     CDS             3149..4795
                     /locus_tag="ACG89C_002339"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4900859.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG89C_002340"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG89C_002340"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4900860.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..>6776)
                     /locus_tag="ACG89C_002341"
     CDS             complement(5976..>6776)
                     /locus_tag="ACG89C_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4900861.1"
                     /translation="DLSVDILRPNLPICREQRILVNILARLSITKIESEETSLEQYKL
                     LKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPL
                     PTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYK
                     KQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPS
                     HIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         2100 a         1261 c         1277 g         2138 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatc
//
LOCUS       DAZUKR010000128         6776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000128 DAZUKR010000000
VERSION     DAZUKR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.130"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(199..777)
                     /gene="pgsA"
                     /locus_tag="ACG89C_002342"
     CDS             complement(199..777)
                     /gene="pgsA"
                     /locus_tag="ACG89C_002342"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4900862.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(986..1909)
                     /locus_tag="ACG89C_002343"
     CDS             complement(986..1909)
                     /locus_tag="ACG89C_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS4900863.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(2648..3934)
                     /locus_tag="ACG89C_002344"
     CDS             complement(2648..3934)
                     /locus_tag="ACG89C_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4900864.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(3927..5195)
                     /locus_tag="ACG89C_002345"
     CDS             complement(3927..5195)
                     /locus_tag="ACG89C_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4900865.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5447..5908
                     /gene="mscL"
                     /locus_tag="ACG89C_002346"
     CDS             5447..5908
                     /gene="mscL"
                     /locus_tag="ACG89C_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4900866.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5968..6414)
                     /locus_tag="ACG89C_002347"
     CDS             complement(5968..6414)
                     /locus_tag="ACG89C_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900867.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(6507..6752)
                     /locus_tag="ACG89C_002348"
     CDS             complement(6507..6752)
                     /locus_tag="ACG89C_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS4900868.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         1911 a         1438 c         1202 g         2225 t
ORIGIN      
        1 tttttttatt taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca gctccttgtt
       61 ccaaataaac agtgttctcc tgccaaaacc tcggtcataa gtcacaatat acataaaaca
      121 tgaaaagctg ttttttgaaa ttatcccttg tgactcagag ttagacggca ggctcacaac
      181 ctctccacta aacgatactt acattgatcc tttgaacaca ccgctgtttt tgatgaagta
      241 atccacacct gaatagatag taaagatcag acaagcataa agcatgattt gatctactgg
      301 aataccgatc aggctgaatg ggatattatt gataaacagc aagataatag caaccatttg
      361 tgtagccgtc ttgacttttc ccggccaagc tgcagccatg acttctccat gttcgacaag
      421 tagtaaacgc aagcctgtga cggctaattc acgacagaca atgatcgcta ctacccaacc
      481 aggggccttt ccttggtcaa ccaacatgat aaaagcagtc ataactagca ttttatctgc
      541 caacgggtcc gcaaacttgc caaaattagt gacaaggcct cgcgcacggg caatttttcc
      601 atctaaccag tctgtgatac tagctaaagc aaaaataact gcacctacta gctgtgtgac
      661 agccagtgtg gtgtctccta cgttaaattg tccccaatct aaaggtaaac ctacaaccaa
      721 aatgaaaatc gggatcatac aaattctaag tacagttaat ttatttggta aattcacact
      781 atcgactcct ttcttcccta ttctacgtga ctatcagcga agtttccacc gatattattt
      841 acttttccaa atgaatttat tcaaaggagt atttttattg aaattgatct ttttgaatta
      901 acgaagaagg accgcgacac agatttgtca cgatccttct tttttatacc tacaagtaaa
      961 cagatgcgta aactaattta tacgatcatt ctgcgtattg aagagtaagg ttcacatttt
     1021 ttggactctg attgttttcc cctggattaa ctggtacgga ttctccattc gctttgatcg
     1081 ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc ggtaacacgg
     1141 tactctgtgt ttcatttgct gccagtgtcc cttgataaac ataggcattg tctactagca
     1201 cacctaacca tacacgattg acagcagtaa attcaagggt tacaggcttg gtagcgtctg
     1261 tgatatttac tgcgatggca ctgccagaat cttctcctgt agctattgcc attttctttt
     1321 cttcacttga ttttgttgtt gattctactg tagaagactc tttggtctgc gtgctttgct
     1381 ctgaggaaga ggactcctcg actgatcctt ctactttaag cgaagaagct gtgtctccga
     1441 ttattggatc agcttgacga tcttgccaag tcatatagcc cacgacaacg acaatcgcca
     1501 aagcaaccaa tcctaataaa atgacaggaa gacttgtcca aaagcgactt ggatgcgatt
     1561 cttcctgatg cattgctttt ctagaagtac ttactgtttc aggctgcgga cgttcaggga
     1621 ccgatcctgt caatttgctt ttcccatcaa aaacatcgac taaatggtct ccatcttctc
     1681 ctacagcttc agcgtattgc cgaataaatg cccgcacgta aaatttacca ggcaattggt
     1741 caaaatcatc attgtcgatt gcttccaaat aacgtttttg aatttttgta atctgctgta
     1801 attcatctaa agatattttt ttattcaggc gtgcttgccg taatttttct ccgatggttt
     1861 caaaagccac tcgttcatct tctccagtct gtttgattct ccgaagcatc tgctgttgca
     1921 acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc ttttttccga
     1981 ataaacattg ttctcctgcc aactcctcgg tcacaagtca caatatccaa taaatataag
     2041 aagctgtttt ttgaacttgt tccttgtgac tcggagttaa acggcaggct cacgacctct
     2101 ttcttaaacg gtgttcaatc agctgttcct cggtattagc tcaaaaaccg gcaaaatata
     2161 aggagatatt tttccgtatg tttctctaat actcaggtca aaacgctgat ttcacaacct
     2221 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa
     2281 cattaagaag ctgtttttac ttggctgttc ttattacttg atgcagggca gctttttcac
     2341 aacctcttgg tacacggtgt tccagaagta gcttcttcgg caataagccg aaatatcaca
     2401 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2461 cacaacctct ttgattactt cgtttgattt tattttaata taagttgatt aaaagagagc
     2521 atgatttgtt ttattttctc actaattttt ccaacacaac taagaatacc ataaaagctg
     2581 attttggagg aggttcctac tacaagtcta aaacgaaatt taagactttt cctcataaaa
     2641 tactggttta ttttggtctc atgtaaaact cgctgaaggc ctcttcactt ataaatgctt
     2701 ccccagctgc cagtacgtct ttcatttgga tcgaatcgat gatttcagga agatcaaata
     2761 aagttcgatc gccaaataaa ctttgcgtaa attgattggc aatatattcg atcgaattta
     2821 atgattggaa atattgtcca agcattttct tcttcagcaa atcgagattt gtttctgaca
     2881 cttccggatc atcagcgaat ccaagaatga tttctttgac tttttctgct gctttctctg
     2941 gttcgtccgt atctccgctg aagtccgcaa agtgaaattc acgatccaag ctgaattcaa
     3001 aaccaaagct gtcatcgata attccttgat tgtacatcgc tagatagttt cgagaagtat
     3061 tgcctaaaat catttggaac aacagattga ttgctgtttt atagcgtaac agttctgttc
     3121 cttcttgcgg caatgtgtct aatcctttaa taccaagtac aaatttatct cgtgtaatgg
     3181 ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt ttcagggaaa taacgtacaa
     3241 tttcttgctt tggagggaaa ttttttgctt cttggttttc ccgaataagt ttcatcaatt
     3301 tctcaggctc catcttacca actacaaata aaaccatatt gcttggctga taaaacgtcc
     3361 gatagcacgt atacaagtct tgagctgtga ttttgtcgat actttcaacc gtgcccgcaa
     3421 tatcgatatg caatgggtgt gtaggataca agttatttag tatgccaaaa aacatccgcc
     3481 agttagggtc gtcttcatac atttggattt cctgaccgat gattcctttt tccttgttta
     3541 cagtttcttc tgtgaagtaa ggtgcttgta caaaatcgat cagagtcgtc agattctttt
     3601 ctacctgatc cgtcgtagaa aacagatagc tggttttcgt aaaacttgtg aaagcattgg
     3661 cggaagctcc ttgtttccca aataattgaa agacatcacc atcttctttt tcaaacaact
     3721 tatgttctaa aaaatgggca attccatctg gaactttcac cttttctttt tcaccatagg
     3781 gaataaattc gttatcgatc gaaccataat tcgtactgaa caaaccatat gtcttgtgat
     3841 agtcattttt aggcaacaaa tacacggtca aaccgtttgg cagtacttca tgataaagtg
     3901 tttcattgat ttgctcatat tctgttttat tcatttgcat ttcctcctgc taaaaagaag
     3961 attgcctgca attcgatttg ctcagctact tgttgtatct ctttgatcgt tacgctctcc
     4021 atacgtttca accattcttc atcagataat tttgtttccg gcagccagct gtccaaaaaa
     4081 gaagcttcga ttgccgcttg cggagagtct agagaaagca gatattggtt tctcagcatt
     4141 gcttttgtct gagacagttc caactctgtt atttcaccat ttcgcaaact ttctaattgc
     4201 tcgtgaatca gatgaagtac ttgatttcgg ttcttttcat cgataccggt ttggacactc
     4261 atgaatcctc ggaacgtatc cacgctacta gaggcataat aagctagact ttctttctct
     4321 ctgacattca tgaaaagttt agagtgagga aaaccgccaa ataaaccgtt aaagaccatt
     4381 agcgcaaaac gttctggttc atcgtaatac acatttgtct gataagctag gttcagcttt
     4441 gcttgaacga ttggctcacg tacttgtcgt tccttcacga tattcacttg aggttgggta
     4501 taaaacagat ctggataaac tgtttcccgc gactcaaatg gtaattgccc aactgcttca
     4561 gctgctttgt ccggatcaac atcgccgaca acaaaaatgt cgatttgatc atttgccatc
     4621 atttcttgat agtaggacgc tagcgaacta gcagtgatct tgtccaaatc ttcgactacg
     4681 cccaaccctg ggatcttttg ttccgggctg ttttcaaaat aaagttcttg caaggcatag
     4741 gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat atagacgcag attgttcttt
     4801 tccaagtcaa acgtcaactg atcaaactga tgatttttaa tatttggata aaataaaacc
     4861 tcttttaaaa aatctaccgc ttgagaaaaa aggtttggat cattgacata ttcaccattc
     4921 acaaaactga tccctgcgtt tagccaatgt aaatttcctt tttttctaac tgataatcca
     4981 aagcttgcgc cataaagctc tgcaagttgc gcacttaact ttgtctgatc cgaataattc
     5041 aaactatttg tttccaacat gctgctgaga agtgaacgtt tcgtgatgac ttcttgttgc
     5101 agacgcgcag taaaacggat aaaaatccgg atcgttttgt attgttttgt tggtaagaca
     5161 tgaagattca cgccttctgc caattgatac accatattta tatgctcctt ttcacagttg
     5221 attgcccgct tactctttta atgggtcaac ctgccttttt tacttattcc tctgcttgaa
     5281 atttataagt tcaggcaatt cgccgttttt tctaaatgat gttacacttt atagtatagc
     5341 atagatgttt tttatcaaaa gaaacagacg gctaaaagcg tatattgttt ttttattttt
     5401 tatttttcga tatactaaag acaaataaaa gaagggaggg tcattcatga tcaaagaatt
     5461 caaagagttc atcatgcgtg gagatgtctt ggatcttgct gtcggcgttg ttattggtag
     5521 tgcgtttaca agcatcgtaa accaagtggt gcaaggcttg atcacaccac taataggttg
     5581 gtttgtcgca atgatcacag gtacttctga tttagaagga gcactttcaa ttcttgactg
     5641 gtcgccgaca aaaggcgtca catttgcgtt tggtaacgtt attagtgcta tcattacatt
     5701 tctgatcact ggttttgtct tattcttagt tgtcaaagcg gcaaaccgtg cgaagaattt
     5761 acgctccaaa gaagagcaag tggaagtgga agaaactcct actgcagaag attatcttag
     5821 tgacatacgc gatcttcttg ccaaacaagt ggcagaagac aatacacatc cagtagagca
     5881 aacaaatctt tccgatactt ctaaataata aagaatctaa aaaaaaagaa agccgatttt
     5941 cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat
     6001 atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata
     6061 aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg
     6121 gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg
     6181 atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc
     6241 aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc
     6301 aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat
     6361 cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca
     6421 aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaaag caaacgaacc
     6481 ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc gatctcaact
     6541 gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt ttcttcagga
     6601 tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt
     6661 tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg accttctaaa
     6721 tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat caacta
//
LOCUS       DAZUKR010000129         6767 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000129 DAZUKR010000000
VERSION     DAZUKR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.131"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..551)
                     /locus_tag="ACG89C_002349"
     CDS             complement(93..551)
                     /locus_tag="ACG89C_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4900869.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(564..1895)
                     /locus_tag="ACG89C_002350"
     CDS             complement(564..1895)
                     /locus_tag="ACG89C_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS4900870.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1906..3348)
                     /locus_tag="ACG89C_002351"
     CDS             complement(1906..3348)
                     /locus_tag="ACG89C_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4900871.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3335..4099)
                     /locus_tag="ACG89C_002352"
     CDS             complement(3335..4099)
                     /locus_tag="ACG89C_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS4900872.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4203..5078)
                     /locus_tag="ACG89C_002353"
     CDS             complement(4203..5078)
                     /locus_tag="ACG89C_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4900873.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5333..5417)
                     /locus_tag="ACG89C_002354"
     tRNA            complement(5333..5417)
                     /locus_tag="ACG89C_002354"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5381..5383),aa:Leu,seq:caa)
     gene            complement(5435..5506)
                     /locus_tag="ACG89C_002355"
     tRNA            complement(5435..5506)
                     /locus_tag="ACG89C_002355"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5472..5474),aa:Gln,seq:ttg)
     gene            complement(5516..5588)
                     /locus_tag="ACG89C_002356"
     tRNA            complement(5516..5588)
                     /locus_tag="ACG89C_002356"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5553..5555),aa:His,seq:gtg)
     gene            complement(5650..5734)
                     /locus_tag="ACG89C_002357"
     tRNA            complement(5650..5734)
                     /locus_tag="ACG89C_002357"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5698..5700),aa:Leu,seq:caa)
     gene            complement(5755..5825)
                     /locus_tag="ACG89C_002358"
     tRNA            complement(5755..5825)
                     /locus_tag="ACG89C_002358"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5791..5793),aa:Cys,seq:gca)
     gene            complement(5871..5942)
                     /locus_tag="ACG89C_002359"
     tRNA            complement(5871..5942)
                     /locus_tag="ACG89C_002359"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5908..5910),aa:Gln,seq:ttg)
     gene            complement(5954..6026)
                     /locus_tag="ACG89C_002360"
     tRNA            complement(5954..6026)
                     /locus_tag="ACG89C_002360"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5991..5993),aa:His,seq:gtg)
     gene            complement(6029..6102)
                     /locus_tag="ACG89C_002361"
     tRNA            complement(6029..6102)
                     /locus_tag="ACG89C_002361"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6068..6070),aa:Trp,seq:cca)
     gene            complement(6109..6189)
                     /locus_tag="ACG89C_002362"
     tRNA            complement(6109..6189)
                     /locus_tag="ACG89C_002362"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6153..6155),aa:Tyr,seq:gta)
     gene            complement(6191..6266)
                     /locus_tag="ACG89C_002363"
     tRNA            complement(6191..6266)
                     /locus_tag="ACG89C_002363"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6231..6233),aa:Phe,seq:gaa)
     gene            complement(6302..6374)
                     /locus_tag="ACG89C_002364"
     tRNA            complement(6302..6374)
                     /locus_tag="ACG89C_002364"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6339..6341),aa:Asp,seq:gtc)
     gene            complement(6384..6456)
                     /locus_tag="ACG89C_002365"
     tRNA            complement(6384..6456)
                     /locus_tag="ACG89C_002365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6421..6423),aa:Val,seq:tac)
     gene            complement(6472..6543)
                     /locus_tag="ACG89C_002366"
     tRNA            complement(6472..6543)
                     /locus_tag="ACG89C_002366"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6508..6510),aa:Glu,seq:ttc)
     gene            complement(6558..6647)
                     /locus_tag="ACG89C_002367"
     tRNA            complement(6558..6647)
                     /locus_tag="ACG89C_002367"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6611..6613),aa:Ser,seq:gga)
     gene            complement(6687..6760)
                     /locus_tag="ACG89C_002368"
     tRNA            complement(6687..6760)
                     /locus_tag="ACG89C_002368"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6724..6726),aa:Asn,seq:gtt)
BASE COUNT         2037 a         1496 c         1104 g         2130 t
ORIGIN      
        1 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
       61 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
      121 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
      181 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
      241 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
      301 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
      361 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
      421 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
      481 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
      541 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
      601 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
      661 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
      721 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
      781 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
      841 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
      901 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
      961 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
     1021 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
     1081 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
     1141 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
     1201 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
     1261 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
     1321 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
     1381 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
     1441 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
     1501 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
     1561 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
     1621 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
     1681 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
     1741 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
     1801 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
     1861 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
     1921 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
     1981 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
     2041 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
     2101 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
     2161 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
     2221 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
     2281 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
     2341 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
     2401 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
     2461 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
     2521 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
     2581 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
     2641 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
     2701 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
     2761 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
     2821 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
     2881 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
     2941 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
     3001 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
     3061 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
     3121 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
     3181 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
     3241 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
     3301 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
     3361 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
     3421 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
     3481 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
     3541 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
     3601 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
     3661 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
     3721 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
     3781 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
     3841 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
     3901 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
     3961 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
     4021 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
     4081 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
     4141 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
     4201 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
     4261 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
     4321 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
     4381 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
     4441 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
     4501 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
     4561 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
     4621 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
     4681 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
     4741 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
     4801 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
     4861 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
     4921 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
     4981 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
     5041 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
     5101 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
     5161 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
     5221 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
     5281 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
     5341 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
     5401 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
     5461 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
     5521 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
     5581 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
     5641 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
     5701 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
     5761 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
     5821 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
     5881 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
     5941 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
     6001 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
     6061 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
     6121 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
     6181 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
     6241 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
     6301 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
     6361 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
     6421 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
     6481 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
     6541 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
     6601 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
     6661 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
     6721 attaacagtc atgcgctact accaactgag ctatgccgga ataacat
//
LOCUS       DAZUKR010000130         6732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000130 DAZUKR010000000
VERSION     DAZUKR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.132"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..254
                     /locus_tag="ACG89C_002369"
     CDS             <1..254
                     /locus_tag="ACG89C_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4900874.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
     gene            complement(424..1386)
                     /locus_tag="ACG89C_002370"
     CDS             complement(424..1386)
                     /locus_tag="ACG89C_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4900875.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1388..2827)
                     /locus_tag="ACG89C_002371"
     CDS             complement(1388..2827)
                     /locus_tag="ACG89C_002371"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS4900876.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3012..4958
                     /locus_tag="ACG89C_002372"
     CDS             3012..4958
                     /locus_tag="ACG89C_002372"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS4900877.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5222..6094
                     /gene="scrK"
                     /locus_tag="ACG89C_002373"
     CDS             5222..6094
                     /gene="scrK"
                     /locus_tag="ACG89C_002373"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4900878.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6390..>6732
                     /locus_tag="ACG89C_002374"
     CDS             6390..>6732
                     /locus_tag="ACG89C_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4900879.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKT"
BASE COUNT         2168 a         1167 c         1197 g         2200 t
ORIGIN      
        1 gaaacaaatt gaaggagtac aaaatctttc aaagcatgtc tcggaaaggg aactgcttag
       61 ataattcact tatggagaat ttttttggct tattaaaaca agagattttt cacgggaaag
      121 tttataactg tttcgaggaa ttaaaatcag ccatcgatag ctatatctat tactacaata
      181 atgaacggat aaaacagaaa ttaaactggc aaagtcccgt ccaatttcgt aaaacaaccg
      241 taacagttgt gtaaaaatat cattttagag catgaaaaaa ggagtggatt tctccactcc
      301 aaaaaagtct aacttttggg ggtcactaca agttgacaac tcctttttgt ttacaaatca
      361 attggatatc tacaaattgt attttctaac gaattttata gacattaaaa aattaattta
      421 acgttaaata cttcctccag aaagtattga gattggtaaa atataatcct tatttgtctt
      481 ttcgccttta atttttttca gaagaatatc aacaataaga cttgcaattt catcaattgg
      541 ttgtcttatt gttgtcaatt gtgggaaaaa tttctcaata aaagaggtac catcgtatcc
      601 aataattttt aaatcttctg ggatattaag tttaagttga tgagcaattt tcatagtgag
      661 aatggctgtt aaatcatcag aaacaaaaat accatctggt ttgtgattta taatagttga
      721 ctttatttcc atttcacgac ggatagtaga cagattatta ggaattttga aaatttttcc
      781 atttggattt tgaaaagaaa aacctagtgc acgtaattct gtgggagaat ccgtgttatc
      841 atttcctgta atcataatgg tattttgaca accatttttt tgtaaagtct tagctgcaag
      901 ttttccccct tcaaagttat ctgaagaaac aatggaaaca ttaggagcta aatttctatc
      961 aaaagcaaca ataggtgctc tcacacgctc ataatcagca attccaagat tatggcttga
     1021 tgagattatg ccatcaactt ggttggcttc aagcatttca atgtattctc tctctttagc
     1081 aggatcattt tgactattgc aaataatcgt tttatatcca tgtttaaata actctatttc
     1141 gagatactca atgagttcag agtaaaagat attactaata ttaggaaaaa taagaccaat
     1201 aagctgaggt gactttcctt gaagtccacg tgctaaatta tttggtttat aaccaagagt
     1261 tttcatagct tcatgaactt tttgttttgt tttttctgat aaatatccct tattattaat
     1321 gacgcgtgaa acggttgttg ggctaactcc agctaattca gcgacatcag ttaattttgc
     1381 taccatttta atattttaat tcaaagtatt ttccagtagg ttcgcctgat ttgatttgaa
     1441 taccattttg gtgattttca gggaatacac gactagtaaa cactttttct cctttattaa
     1501 taaaaatctc aatgattgag ttatctacaa aaatgttagc agttgtttct ttagtatcaa
     1561 ttgagcattc acgaatcgta ccaaagtcag tggcatattg aatgcctgct ttgctgcgat
     1621 ctaaaataat ttttccttct ttggtatcta caatcaagct aagtccattt cctttactat
     1681 cagagaaaag aagtaactcc gatttctgat ttggaggaaa agttagttca agctcataag
     1741 tattatttgt ttctgctttt gctgtaaatt gttcagaact agcacgtaat gatgtaatag
     1801 cttctactgg atattggtaa agtttaccat cttttattga aagttctttt acaagactca
     1861 ttgctccctg ataatcatac ttatcagttg gataatcgat atcaggtagt ccaatccaac
     1921 tgacaatcag agcacgacca tcaggagtat tgaatccttg agtcgcatac gcttcaaatc
     1981 cataatcaag attttgaatt tctgaagcgc ccacaagttt tactttttct atgtcaagcg
     2041 attgacagat cttaaaagta ttcggataga tattcccata atttagctca gatttatcca
     2101 acccttgagg acaataaatg agaacaggtt ttttgtctac aaatactaga tttggacact
     2161 caatcatgta ttctgaacca gtgccgccaa aatcaaggtt gccaacttcc tcccatgttt
     2221 ccacgttatt gtcatccgct ttgtaaagtt tgataaagcc tgatttttct aaattttgtg
     2281 ctccaacaat cgcatacaat tgtccttcat aattaaacat ttgtggatca cgaaaatgat
     2341 cagttacatc agcaggttta tgaataagaa cattctcacc tttcgtaatg ttgtagtctt
     2401 tatccatcca tgctccgatt tgaagtgtat cacgttccca attttcattt cgaacgtttc
     2461 ctgtatagag taagaataga ttcccctcta tttcatatgc agaaccggaa taagcgccgt
     2521 ggctatctcg ttttgtgtct ggaagaagtt ctgctcctgt tacatgaaaa tgaactaaat
     2581 catctgattc attgtgtacc cattctttca acccatgagc agctccaaat ggccaatttt
     2641 gataaaaaag cgtatatttt ccgttaaaaa aagaaaaacc attaggatcg tttaacaatc
     2701 ctgttttagg ttcgatatga tagctagcac gccaaggtga tcgttttaca ttatccttaa
     2761 ttttatccat ctcatcttta gtccaatcag aataagcacg atatcgtatt tctcgaggta
     2821 agttcatatt ttactccttc aatttatctt tttacaacta gtatggtaaa cgttttccgt
     2881 tgaaaaatca atcatttcat ttaaaaaaat aaactatatg acaaacgttt gacatatttt
     2941 taagatatgc taaaataaat ttgcatttca gtgaaccgtt ttcacgtaaa aataaaaaag
     3001 gagtgattcg aatgaattac agtaaagtag cttccgacgt aatacaagcg gtcggaaaag
     3061 ataaccttat tgcagctgca cactgcgcta cacgtcttcg tctcgtacta aaagatgaca
     3121 cgaaagtgaa tcaaaaagct ttagacgaaa atccagatgt gaaaagcaca tttaaaatta
     3181 atggacaata tcaagtgatt atcggtgcag gtgatgttaa tttcgtttat gacgaactta
     3241 tcaaaaaaac tggactttcg gaaatttcaa cagatgattt aaaacaaatt gctagtaaag
     3301 ataaaaaatt caaccccatt atggcattaa ttaaattatt atcagatatt tttgttccaa
     3361 ttatacctgc ccttgttgca ggtggtttat taatggcatt acaaaacttt ttaacagctg
     3421 ctggactttt tggaccaaaa tcaattgaag aaatgtatcc tgcgattgaa ggtatttcct
     3481 caatgatcca actgatgtcc gctgcaccat tcatgttctt accaatttta gtaggtattt
     3541 cagctgctaa acgttttgga gcaaaccaat ttcttggtgc tgcaattggt atgatcatga
     3601 caacaccaga tcttggtggc tcaactgaat attggaatat ttttggttat catgtttcac
     3661 aaacaaacta tgcttatcaa gttattccag ttttagcagc tgtttggata ttatctatat
     3721 tagaaaaatt ctttcataaa aaattgccat catctattga ctttacattc acgccattat
     3781 tatcagtaat gattactgga tttttgactt tcacagttat tggccctgta atgttattgt
     3841 tgtctaatgg aattacagac attattgttt ggctatataa cactactgga tttattggaa
     3901 tgggtatttt tggaggaaca tattcactta tagtcatgac tggtttacat caatcatttc
     3961 cagctattga aactcaatta ctttcagcgt ggacaaatgg tatcggacat ggagacttta
     4021 tctttgtagt tgcatctatg gcaaacgttg ctcaaggtgc tgcaacattt gccatttggt
     4081 tcttaactaa aaattctaag acaaaaagtc ttgcttcctc tgcgggtgtc tcagcacttc
     4141 ttggaattac agagcctgcc ttgttcggag ttaacttaaa ataccgcttt ccattctttt
     4201 gtgctttaat tggttcagga attgctacag cagtagcagg attacttaaa gtagttgctg
     4261 tttcacttgg ttcagcaggt ttcttgggat ttctttcaat taatgcaaca tctattccat
     4321 tttacgtatt gtgtgaattg attagctttg tagttgcttt tgcaattact tacttctacg
     4381 gtaaaacaaa agctgctggt atctttaaag cagaagaaat tgctgaacaa acaccagtta
     4441 attcttcaaa agtagcaaca acagatccaa tatctactga tgttgaatca acacaaacaa
     4501 aagtttcatc agaaacaatt gttagtccca tttctggtaa agtgattagt ctttcttcag
     4561 taaatgatcc agtcttttca tcagaagcta tgggaaaagg gattgccatt aaaccaaatg
     4621 gaaacacggt ccattctcca gtagatggta ctattcaact cacgtttgaa actggacatg
     4681 cttatggtat taaatcaaat aatggtgctg aagttctaat tcatattggt attgatactg
     4741 tatcaatggg aggaaaagga tttacacaaa aagttactac taatcaaaaa gttagaaaag
     4801 gcgatattct tggagcattt gaccgttcaa tgattgagaa agaaggtctt gatgatacaa
     4861 caatgattat tgtgaccaat acttcggatt attcagaagt aaatatagta gctgaaaata
     4921 cagtcactga gggtactgat ttacttgaaa tcaaataaat atatttccct gagtaaagat
     4981 ggaattttct ttccaaaatc aaataatata gattaatcaa atatttgaga ataacatttt
     5041 atttaatctt ttcatatatc aattgtatga aaaattatta gaattttaaa aaatgataaa
     5101 aattcaaaaa gaaaaaatag tagttattta cttgtcatgt tactaaataa aaaaataatt
     5161 taaatatgat atatagtcat ttatttagtg atttcattct aaaataaagg gagaaatgtc
     5221 tatgctatac ggtgcaattg aagcaggcgg cacaaaattt gtgtgtgcca ttggcgatga
     5281 agaaatgacg atcaaggaaa gagtgagctt tccgacaacg acacctgagg aaacaatgcc
     5341 tttagtcatt gacttcttca aacaatacca agcagattta gcagggattg ggattggctc
     5401 atttgggcct atcgatatcc atcgcgattc cgccacttat gggtacatta cctcgacccc
     5461 gaaattggca tggcaaaatt ttgatttcat cggtaccatg aaaaaagaat ttccgatccc
     5521 gatttcttgg acaacagatg tcaatgcggc tgcctatggt gagtatgtct tcggcagtgg
     5581 caaaggattg agcagtgtcg tttactatac gatcggtacg ggtgttggcg gcggtgcctt
     5641 acaagaaggg cgctttatcg aagggtttag tcatccagaa atggggcata tgctcgtcgt
     5701 ccctcatcca aaagatgact ttgctggcag ctgtccattt catggcaact gtttagaagg
     5761 gatggccgct ggacccgcca tcgaaaaacg cttagggaaa aaaggacaag aggttgcaga
     5821 agatgatcct tattgggaaa tcgaagcctc ctatattgct caatgtgcct acaatacaac
     5881 cttgatgttt tctccagatg tgatcatttt tggcggtggt gtgatgaaac aacgccatat
     5941 gctgcaaaac gtccacgacg catttgcaaa attggtcaat gggtatgtcg aaacacctga
     6001 gttaagcaaa tatatcgtga ccccagcatt agaagacaac gctggaacgc tcggttgttt
     6061 ggcattggct cgagaagcgg tcttacattc ataaacgaaa aaaggctctt cgacatttag
     6121 tgttggtaga gaaagataca actgaaaatt tagtcataac cagttaggca gagtgcttaa
     6181 ctggtttttg ttttacttgg aagatcaagc cttgaatgag ataaattctt gctcgaaagt
     6241 tatggaaatt tcgatagccg taggccactt gtattgtgca atagtttttt acacgccgtt
     6301 tgtataatta agcgagacaa ataaaaaagc catttatgtt acactcaaat cgtaataccc
     6361 gctaaagcat acaaggagag tgaacataaa tgacttatac ccatcttaca ccaaacgagc
     6421 ttgtaatgat agaagcatat tttcatcaag aaactccggt tgctatcgtt gcgaagcagc
     6481 ttaaacgtgg acgccaaaca atttacaatg tctataactt tctcaaatgt ggtggaacag
     6541 cacttgaatg ctttgaacaa tacaaagaaa ataagcgacg ttgtgggaga accgaaatca
     6601 tttttcctgc tgaggaaaaa gaatacattg caaaaagatc aactgaaggt tggaccccag
     6661 acgtcattat tggccgtgca gagcgaacct tctcttgttc agtaagtacc ctctatcgcc
     6721 ggtttaagac gg
//
LOCUS       DAZUKR010000131         6728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000131 DAZUKR010000000
VERSION     DAZUKR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.133"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG89C_002375"
     CDS             <1..435
                     /locus_tag="ACG89C_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4900880.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG89C_002376"
     tRNA            complement(759..842)
                     /locus_tag="ACG89C_002376"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG89C_002377"
     CDS             1370..1870
                     /locus_tag="ACG89C_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4900881.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG89C_002378"
     CDS             complement(2060..2404)
                     /locus_tag="ACG89C_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS4900882.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG89C_002379"
     CDS             complement(2558..3478)
                     /locus_tag="ACG89C_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4900883.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG89C_002380"
     CDS             3665..4156
                     /locus_tag="ACG89C_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS4900884.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG89C_002381"
     CDS             complement(4180..6555)
                     /locus_tag="ACG89C_002381"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4900885.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
BASE COUNT         2072 a         1337 c         1127 g         2192 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaact
//
LOCUS       DAZUKR010000132         6643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000132 DAZUKR010000000
VERSION     DAZUKR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.134"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG89C_002382"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG89C_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG89C_002383"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG89C_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS4900886.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG89C_002384"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG89C_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG89C_002385"
     CDS             complement(2143..2373)
                     /locus_tag="ACG89C_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS4900887.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG89C_002386"
     CDS             complement(2603..3421)
                     /locus_tag="ACG89C_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4900888.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG89C_002387"
     CDS             3582..4271
                     /locus_tag="ACG89C_002387"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4900889.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG89C_002388"
     CDS             4285..5787
                     /locus_tag="ACG89C_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4900890.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG89C_002389"
     CDS             5800..6276
                     /locus_tag="ACG89C_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4900891.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG89C_002390"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG89C_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAZUKR010000133         6629 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000133 DAZUKR010000000
VERSION     DAZUKR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.135"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..2820
                     /locus_tag="ACG89C_002391"
     CDS             145..2820
                     /locus_tag="ACG89C_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS4900892.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3002..3241
                     /locus_tag="ACG89C_002392"
     CDS             3002..3241
                     /locus_tag="ACG89C_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900893.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3257..4630
                     /locus_tag="ACG89C_002393"
     CDS             3257..4630
                     /locus_tag="ACG89C_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4900894.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4634..6268
                     /locus_tag="ACG89C_002394"
     CDS             4634..6268
                     /locus_tag="ACG89C_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4900895.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6431..>6629
                     /locus_tag="ACG89C_002395"
     CDS             6431..>6629
                     /locus_tag="ACG89C_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900896.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2323 a         1071 c         1330 g         1905 t
ORIGIN      
        1 tgatttccaa aatcacaact tttttctttt gtcaagattt tgagtgggat atatttttgt
       61 agaactgaag atcatgaaat tatgagaggt tggaggtaat gtagtcatac aaataaaaat
      121 tttttagaaa gtgagtggta attaatgaaa atgaatcaca cttcaattca gaagcattta
      181 caactgttaa atacactggg gatttcttat gttaatttga gaactttttc tacattaaac
      241 aagaaaaccg tttctcatat tttttctact gcagattctc aagcaattat taactattta
      301 gaaaaacaga aattgtctga aggtacagta aacataactt ataatcctat taaacaagag
      361 gtgattacac aaaaaaagca ttctgtgaga gacaatgata ttgaaaaaat tcggtttgta
      421 tttattgata ttgaccctaa aaggaaagag ggaagtgcaa cagatagcga aaagaaaaag
      481 gcagaaaatg ttatggaaca agtagaacgt tatttgaaag aaaagagaat agaatcattt
      541 gtaaaagttg atagtgggaa tggctaccat atttttcttc ccataaatga acaaccaaat
      601 aatcacgaga cgattcttac tattcagaat tttcttcaat tacttcaccg aagatttgga
      661 gtagaagatg aagttgatat tgatacttct gtttataatc cttcaagatt atgcaaatta
      721 gttggtacac cagcggtcaa aggaaagtcc acaaaagagc gtccgcatcg tttatctagt
      781 ttcctttcta ttcccgataa catagataga agtaattgtt ttaaaattct tgaacagata
      841 gttgaagaaa attccatgat gtcaacctct agagtaaaaa gccatccatc ttttagagaa
      901 gtgttacctt ttattcaagc agactataaa caatggttag ctcactatcc tgaattaact
      961 tataccataa aagagggcga tcataaagga gttaccttac ttatatttga cgaatgtcca
     1021 ttaagaaaac acacgaataa ttctaatggt tcttgtttgt ctgttacggc tgataataag
     1081 attcgatttc attgtctgca tgcttcggat aaaaataaag atattcaaga ctttgtaaaa
     1141 aaatatccgt tgccaaaacc tgtaaatcct tctgtagaat cattagaatc caatcaagaa
     1201 tattattttg accagtttaa actgaatcaa gaaggagtat ttttggtgaa agaaggtcaa
     1261 gaagaaaaag ttgcttgtcc gatgtttatt aagcaaatac gcaaaaatat cgaacaaaaa
     1321 gtcattacac ttgttcttgc ctattatgtc gatcattgtt gggatgaaat agaaatagaa
     1381 ggtggatgtt tacaaactaa taattttaaa caattagcaa aacatggctt aatcattaaa
     1441 gctagaagag aagctgaagt cgttgacttt ttacagattc aacgggagaa tgctccaatc
     1501 ttttttgaac atgaatctct tggctggaaa aaagaaaatg aagcaatgat ttttcagtta
     1561 caagattctt ttggacgtga aattccttct gtattaacta aaaactcatg ctaccaatta
     1621 acaactaatg gtacagaagc ggaatttgac gcattggttc ataagtatgt tttaggaacg
     1681 aacttagaac ttgcttggtc gattggtatt gcttctattg tgttaggcta tttaaacgtt
     1741 acgaaggcta atactttttt gagtttacta attaacctac agggcaagag cacaacgggt
     1801 aaaaccacta cgttacactt tattgcctct ttatttggaa atccaactac ggttcttaga
     1861 aatatgaatg ctaccaataa agccattata aagctagcat caaacaatca gggaggtgtt
     1921 ccactaatac tagatgagtt aggagcagca actcaaaata actttagctc gttgatctat
     1981 caattagctt caggagaaga aaggctaaga ttagattcta attgtcagct tatgccacaa
     2041 gagacttttt ctgttttgac ccttacctct tctgaagagc gtttggaaaa ttatttcaat
     2101 gagaaattaa ctggtttgaa agttcgatat atcgaatttt ctgatatcaa gtttacaaag
     2161 gatgcgaatc acgcagagaa agttaaaagt ctttcttcta atacttttgg ttggggtata
     2221 aagttatttc ttttgaaact ctttgaacaa gggattacaa aaattgaagc aacttttgtc
     2281 gaagctcaaa aaaaactatg ggctgtttta cctgaacatt ctttgaagca acgcattggt
     2341 aataatctag ctttgattta tagtggagcc ctcctaattc aagatatact gggctatcct
     2401 ttacgattag agcatataca gacggtgctt attaattcgt acgaacagtt aattgtagat
     2461 actagtttag gaagtgagca aacggtattt gaccgagtga aaacattgtt ggtgatgaat
     2521 agccataaat ttttaacaaa cagtgacaac aaacatacgc ccagtacgat ttggggaaaa
     2581 gtatcaattc aaaaaaatgg ggatattaag gtaaatattt ttcctatccc gtttgaggac
     2641 ttgttaaggc gagaatttca agtaaaggat ctaacctatc tttttaggca attgcttgat
     2701 tcgggtaata tgtgtgcaga aaaagggaga agaacgaaac gtattcacat gaatcgtcaa
     2761 gttcttccaa catacgagat tctattacct tcttcactca aatcgtattt tcgattgtaa
     2821 tttagaataa accaaagtat gaaagtgagg caccatgtta tggaaatatt tcttattgat
     2881 ttagcaaaac aactgattac ttatctgtat gatagcgagc aaacccaaac ggaagtaaat
     2941 gaagcttgat ctatgcttca tttacttcat tcaatcaaac atgaaagaaa ggaaaattct
     3001 aatgactaaa aataaaatga cactaaaagc agaggttctt ctgtatattc aagaacattt
     3061 ttcaaatcaa gcctttttta caaaaccaat ttacttggat ttcgaaataa gaggggtatc
     3121 tgctggatct atcgggggta ctcttcaagc gttgaagaac gaaggatatt tagaaaatca
     3181 ctttgtgcaa cgttcgttca atagaagagt cgtgaaaaaa tggtatttag ttcattcata
     3241 gagaggggaa tcaagcatgc gtgtggcttg ttatgatcga acgtctgttt atgaagaagc
     3301 gaaaattagt aatgacgctc aatatgatgc agtgaaagag tatgttttag agaaatatga
     3361 acaaattccc caacgatttt cagacaaagg agaatcaggg gaaaaagcgg agcgtccagc
     3421 ttttcgacag cttagagctt gggtttgtgc gtcagaagaa gagggggtga tctatgtttg
     3481 gcgttatgat cgattggcac gtcaagccaa aatagcctta gaatttattg agttgtgcca
     3541 acaacatcag gttcaagtgt attcgatttc tgaacctctt cctgcttctt tctcacacgc
     3601 gtcaacgaat aaactatttg tgcaattatt gttcactttt gcagaacaac aacgaaacgt
     3661 cattattgag aatgttcgtg ccggactttc ctataaaaag caacaacggc aatatttgtc
     3721 ttctgcggta ccatatgggt atcgtttagt gaatgggaaa atccaacaag aagtacacga
     3781 agcaaggatt gttcgctata tttttcaact ttatttgacc aaaaaatatg gctacaaaaa
     3841 gctttgtcaa cgattgacgc aacaaaagct tttcttccgc gaacgtcctt ttcagcccta
     3901 tcatatttat agtatcctaa aaaatccatt gtactatggc gaaatcaaag gaggatcttt
     3961 ggggaagtat ctaggaacct ttgaaccgat tctctcgaaa gcaacctttt tacaagccca
     4021 agagattcgc caatcaaggt gtacagctaa aaaagatacg tatctgtact tattacgtca
     4081 aaaaattcgg tgtcccttct gtggtcgtca tctgagttcg aagtatcaat ggaatacaaa
     4141 gaagacgaag acgcttcatt attatcattg taccgaccga agctgccaag gaatttttct
     4201 tcgggcagaa agggttgagt cagaagtatt ggaaaagata caggcttttt ttcaatacga
     4261 aggcgtctat caagccttga ttgcccaatt aacgcaacaa ttagccgaaa agcgaaaaga
     4321 tgaaaaacgc aaagtaatcc aacaaaagaa acaagaagca caatggatta aggcatttga
     4381 agagggcaat tgttcactgg aagaactcaa gcaacaactg caagctcttc aaaagccgat
     4441 gtctacttct tcgtttcttt cactccaaca gtgtgaagca aaagtgaaag aatggcttca
     4501 actaaaagac ctctctagtc gacaattatt atgtgactat gtttgtcgga ttgaggtaac
     4561 aaaggataaa acagtttgtg ggatttactt ccgacaactg acacaaaata ttttagaaag
     4621 gaacgaatga tctatggaaa tgatacaaac caaacgagtc gcattgtata ttcgcgtgag
     4681 tacacaagaa caagctttag aaggacatag tattcaagca caagaacaaa atggacgaca
     4741 gtttgccgaa cggatgggct atgaagtggt agaagtttat gccgatgaag gaatcagtgg
     4801 gaaatccaca aaaaatcggt tcgcctatca acgaatgatg aacgatgctc ggaaccatcg
     4861 ttttgatcta ctagtgattt ggaaattgac ccgtctcgga cgcaatatgt tagatgtctt
     4921 acaaacagtc gaggaattat tgcgatatga cgtaggatta cattcgatct cggaacaatt
     4981 tgacgtgacc acttcgacag gtaaattgat gttacaactc cttgggagtt tcggtgaatt
     5041 tgaacgcaac caaattgcgg agaatgttca aatgtcaatg caaagcttag tgaaagataa
     5101 aaagcgttat gcaggaggcc gacgtttagg ctatgtcagt ggtattgaca cgaatggtca
     5161 aaaacaattg gtcattgaac cagaagaggc gaaaattgtt cgtctgatct atgcaaaata
     5221 tttaggcgga gcagggtatc gagcgattgc caattttttg aatcgtcaag gatatcaaac
     5281 agtcaaaaag aatgcttttt ctaccacggc agtcaaagat atcttacgca ataaaattta
     5341 tgcagggtat ttagaatatg cgcgctacat caattgggaa accaaacgac ggaaggggaa
     5401 gaatccgaga ccgattttag tgaaaggaac ccatgaaccg attatttcgg aagaagatta
     5461 tcaaaaagta caagaacgcc ttgctttaga aggaaaacaa cccaaatgga accatacagg
     5521 tgaaaatgta ttgacaggct tgttacgttg tccagaatgt ggcgcaccga tggcagcgag
     5581 taatgtcaca aataccctaa aggatggcac aaagaaacgg attcgatatt attcttgttc
     5641 agtctttcgg aataaaggag caagtgtgtg tcatgccaat tcgattcgtg ccgaacaagc
     5701 ggaaacattt gtcgccgacc gattaaagga gatcgttcag ctaccagaag ttcttccccg
     5761 attggtggca gcgttaaatg aagaaatagt gcgacaaagc caaccattgg aacaagaact
     5821 cgtggtgtta ctggaacgaa aggaagaatt gaagactaaa attgaaaaat gggaagctgc
     5881 tttggaagat agtcctgaat tgtttccgat gttaaaagac cgcttagacg aattgactga
     5941 aaaacgacga cagttgcaca ttcgagaaaa tgaaatctta gggatctttc aacaaggaga
     6001 gccgattcaa gtcaaagatg tccaacggat attgactaat ttggatcgtt ttcttgccca
     6061 gagtgagaag aagcaaatca aagccttgta tcgtaccttt atagagaaaa taacctttga
     6121 tcctaaccat aaggagatgt tggaaattac gatgaaattc acgcatgccg tggtacaaca
     6181 attgaatgag caatatcaaa tagccgtatc caaaacaaag gatacggcta tttttgtgtt
     6241 aaaaacaccc tttacgttaa cgatctagtc taggttatat caaacaacgt tagaggggcg
     6301 ctaacgcttc tctagcgatt tgaaatcgtt agagaagtag ttagggaggt ccttatcata
     6361 aatagggata tttattaatg aatatttgaa atataattgt atataagaat taaaaagagt
     6421 ggggatagat atgaaaaaga tgggtaagtg tattttatgt gaaaaataca ctgaacttac
     6481 taaagaacat gtaccaccta aaaaatcagg gaatactgga ggtaaaaaga ccagaactgg
     6541 taatttagat gatttcctta agagcgattt tacaaaaggt gattttccga aggggattaa
     6601 aaggaaacct caaggaaatg tttattata
//
LOCUS       DAZUKR010000134         6566 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000134 DAZUKR010000000
VERSION     DAZUKR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.136"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..808)
                     /locus_tag="ACG89C_002396"
     CDS             complement(<1..808)
                     /locus_tag="ACG89C_002396"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4900897.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQG"
     gene            complement(840..2291)
                     /locus_tag="ACG89C_002397"
     CDS             complement(840..2291)
                     /locus_tag="ACG89C_002397"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4900898.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(2335..3099)
                     /locus_tag="ACG89C_002398"
     CDS             complement(2335..3099)
                     /locus_tag="ACG89C_002398"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS4900899.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(3127..3825)
                     /locus_tag="ACG89C_002399"
     CDS             complement(3127..3825)
                     /locus_tag="ACG89C_002399"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS4900900.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3837..4616)
                     /locus_tag="ACG89C_002400"
     CDS             complement(3837..4616)
                     /locus_tag="ACG89C_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS4900901.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(4632..5576)
                     /locus_tag="ACG89C_002401"
     CDS             complement(4632..5576)
                     /locus_tag="ACG89C_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4900902.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5622..6401)
                     /locus_tag="ACG89C_002402"
     CDS             complement(5622..6401)
                     /locus_tag="ACG89C_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900903.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         1783 a         1413 c         1059 g         2311 t
ORIGIN      
        1 gtccttgtac aaatccagta aatataattc gctcatcacc ttttgctaat tcctttagtt
       61 catttgcaaa ttcatctgta tcagagcttc ctccggctat tataagtttt ttatctgtct
      121 gaacatcttt aaatgcctca attagatacc gaattccttt ttcgggaacc aatcgcccca
      181 aaaacaaaat ataagaatct tttgttaatc catacttttc tgtaatcaat cctgctgatt
      241 ttatttcaat ttttttaacg ccatttggta taaaccttgt ttttcttcca tatatatctt
      301 caaaataatt ttggacgctt tcactaagta caattatttc atctgcaaat tttacagcac
      361 acttttcccc taacatgatg tatgtactag caagcttgtt ccatttttct ctttgatgat
      421 ctagaccatg aattgttgca acacatcttt tcccaaaaag ttttggcaac cacaacatag
      481 cacatggtcc ttctgcatga aaatgaacaa catcatattt tccgaatgct gctctaattc
      541 caccaaatac tgaagaactc attgctgcaa ttccttttat atttaatgta aaaatagtct
      601 ttaattttac acctttatat tctttatttt tatgctggtc aaattccttt ccgctaacat
      661 gatgaccact tcgattatag caagtcactt catgcccaag tgcaaccatt cgcgttgcca
      721 attcttctac cactatctca attccacctt ctcttgatgg aattctttta tgacctagca
      781 tacagatttt taattttttt cttgccatca cgttttactc cttctatagt atctttatct
      841 tacatggaac cgtttctatg aagcacagtt ttgactgtcc taaacaaaat tctaaaatct
      901 aatcccatac tccaattcct gatatattct gcatcaagtt ttacaacctc ttcgaagtct
      961 gtgatatcac ttctaccact cacctgccac attccagtaa tcccaggttt catcgcaatt
     1021 ctcgcacgat gatgaaaatc atacttctca tattctgact tcaagcaagg tctaaacccc
     1081 accaggctca tttttcccat taggacatta taaaattgtg gaaactcatc caatgatgta
     1141 gatcgaataa attgtccaat tcctgtcttc tttgttccat caggaagtat tttattgcca
     1201 atgattcttg ggtcaaaatc catcttaaac atgagattat tactcatctt attttctttc
     1261 atcaactctg ctttgcgttc ttctgcatcc atatacatac tacggaactt atacatctta
     1321 aatgtcttac cattttgacc aatacgttct tgtgcaaaga aaatcggccc tggtgatttg
     1381 atgtaaataa ctggtgctac gaagataaaa ataacacctg ttgcaataca tccaataatt
     1441 ccagcaagaa tgtcaatcgt tctctttata aatgcttgcc tcatcgtcat aacattaata
     1501 ctagatgtaa taacagtata atcaccaact cggccaataa attgttttcc acgtgtacta
     1561 tcagacactt ttgtaagatt catatgtaca gcaagtccca tttccgaaca tctggcgatt
     1621 aattctttgg gatatggaac actttcgtca acattgatga atacttcatc tacccaattc
     1681 tgtaaaagat attccgctgc attatcagca tttgcaacta ctgggacacc tccgatggta
     1741 gttccataca tatctctgtc tactacaatc acaccactaa gtttatacat gccataattc
     1801 ttctctttaa gattacgaac tacatttttt gcaatctttt gagatgttac aataactaac
     1861 gatacatctt caccgttttt catgcggtga tgcaacaacc ctttccaaag tatacgaatt
     1921 atatatgtta ataatccata caccaccccc attataaata aagaaactct ggaataatca
     1981 tttccatcct gcattgtaat aaggtataaa ctgcacacaa gtaacagcat aaaagattgc
     2041 tttatagaag ctacagcctc tttataatat cctcttttta aaacattttt atatgcctca
     2101 aataaaaata taatcgtaag attctcaagt tctataaaaa ttgccatgtg gcgataaata
     2161 ggaatcacat acggattcca gtcaccctgt cgcattacat atgatatata aaaagcaagc
     2221 tggacacaaa ataagtctaa taaaataaaa tcaatatgtt tagaccaacc acttgagttt
     2281 tttctataca tctctcactc tctcctcatt tcctttttat ctacgaataa agaactaaaa
     2341 taaaccaaac ttcttctttt ttacctcttc atagattgga taattgatct catccccgtt
     2401 gatcagatcc tttgcgactt gtttcatctg tactactttt tccttaccaa actccttggc
     2461 aatctgctca tagcactctt ttaagtaaaa ggtgcgctta gagaccccgt gtgcatcaga
     2521 agcagccatc tgcacgagat tatgtttgac cattgttttg gctgttttct ggatatcttt
     2581 gccaaataca ccaacataac ttggcgcagt aagttgggcc aaacatccca tatctaggaa
     2641 cggaagcaaa tgatttgggt cttctcgaaa atgcgcgttg cgttctggat ggacgatcac
     2701 tggtatcttt cctagttcta acaactcaaa aaaaagccgt tctgcatata acggcacttc
     2761 cagtgtcgga aattcgatca gaagatatgt atcatctaaa tctgtaaata aaatttcatc
     2821 tcggtggatg tcttcgatga gcgttccggt aatacgtact tcttgtcctt ccaataacgt
     2881 caaaggcaac tgtcttttct ctaattcggc ttgtaacgaa gcgactagag aaatgacttg
     2941 tgatttttca ttgctgtatt ttccattatt gtggtgtggt gtgcataaaa tatgcgtaat
     3001 gccttgctga atcgcttttt cagccattgc aatacttgct tctaagtttt ctgccccgtc
     3061 atcgattcct ggtaggatat ggcaatgtag atcgatcatt tctcttgtct cctctcttct
     3121 tattccttaa tttccgtagt aataaccagc atctttgctg tgttctgctc cgttgtatac
     3181 gacacctaag actcgtgctt gtaccatatc taacagttct tttgctttag tcaatgattc
     3241 ttttcgtgac gtattttccc gtacgaccaa taaggtgcca tccgtttttg aagacatgat
     3301 ctgtgcatct gttacggcaa cgactggcgg catgtcgaag atcactacat caaatagttg
     3361 tcttgcttct gctaaaatct gatccattct tggcgaactt agtagttctg atggatttgg
     3421 tggtttaggt ccacttggca gaatcgacaa attgtcgact ggtgttcgtt ggatctcatc
     3481 tgctacactt cctgatgaac taagaacagt acttaatccg cttgcgttgt tcaacttgaa
     3541 cgttttatag atgacgggct tacgtaagtc tgcatcgact agcagcaccc gttgaccaga
     3601 tttggcaaaa acaacggcaa tgtttgctgc agtggtcgat ttcccttctc caggtcctga
     3661 tgaagtcaca acaatcgttt tgatttgctg accagctgcg gaggcaaact ggatattcgt
     3721 ccgaatcgta cgatattgtt cagcgattgg tgaagaggga ttggtgagtg tgaccaaact
     3781 gactgcatga gtttgggctg ctttttgttt ctgtgttcgt gccattttct ttcctcctag
     3841 actcttgagc ggcttctgcg tgatggcgct gttttatcgt ttgtagcttt tggttgtgtc
     3901 tgagctggtc cttttttacg gataccagca ttcaattctt tcgctgacat ttctggaact
     3961 gttcctaaga ttgggaatcc taatgtatct gtcacatatt tttcatcttt cactgtccga
     4021 tcgagtaatt ccaataagaa tgccagacct acacctacca tcattcctaa cattaagcca
     4081 gtcattaaat tcaatttatt atttggcgaa acaggtgtcg tattggcaac tgcacctgaa
     4141 atgatcgaga ttttatcaac attcatcaca tcttttgcgt tctcttggaa aacttgtgaa
     4201 gttgtgttag cgatctgttg agcagccact gcattcgtac ttattgcctg aatcgagaac
     4261 atttgtgaat tttctgcctg attaactgag atggcatctt tgatttgttc agcagtcatt
     4321 ttcacattgt cttctgtttc taaacgatct ttgacttcat tgagcactaa atcactgacg
     4381 atcatatctt tatacgtatt gatcatttgc aggttcgtat tgacatcatt gacatttgca
     4441 gtgtctgatt gtggcagtgt cacgatcaat tgtgcttgtg aactgtattt tggtgtcaag
     4501 atgaaaaatg ttacgattcc tgagatggcc aagccagcaa acattgagat gatgattatt
     4561 accaagtgtt tgcgtaatgt ttcaaaaatt tctttcaagc tgattgtctc ttccattttt
     4621 tctccacttt cctatttatt ctctagattc atttgatctt ttaattcttt tttcacacga
     4681 tcaagttctt gatcactgac tttttgatag gagatattat cttgcatgaa gccttctcct
     4741 tgcagctgat cttgcttaat cgtaccaaat gcctcccgat aatctaaagc aatttttttc
     4801 atttcatcaa agttcatgtc tgttttcata ttcgactcta gagcattcaa aacggattga
     4861 tagttgctga caccgtttag actcaatact tttttcgcta cgccttctac gatctttctt
     4921 tggcgccctt gtctaccgta atcaccattt gggtcttcat aacgcatacg agaataagat
     4981 aatgcctgat ccccgttcaa tgtaatttta ccgattggaa agtcatagcc atcttgtgag
     5041 aaagtgaggt cattattgac ttcgatacca ccaactgcat caacgagttc ctttagccct
     5101 ttcatattga tagagacata gtgatcgatt gggatgtcta catatttttg tacggtatcc
     5161 atcgccatgg cagctccgcc gaatgcgtaa gcgtggttga tcttgtcttt tgtgtcacga
     5221 cctacgatgt cgacatacgt atcacgagga atgctgacga tcgttgtctg tttgtcttca
     5281 ggactgactg tggcaaccat catcgtatct gatcttcctt gatcgacccg acctaaatct
     5341 ccagtatcaa tccccatcaa tagaacagag aaagattctt gtttgtccag atcaactggt
     5401 ttcgttcgtt ctccttgggt tcgttccact gattcgtaag ttttctcaat agaatgtgag
     5461 agatccagat ataattttgc tcctactcct acaattgcta atactaatac gataagaact
     5521 gctaaaataa tcagaaatat ttttttccct cttgattttc ttcttcgtct tgccatagtt
     5581 tcctccgttt gctaaccttg cttgaatcaa cgttttaact gtcaatagct tatattgcta
     5641 tagcccattt gctcagcaac agtttgtgct tttgttgcgt atgctgcgct tattttttca
     5701 ttccggatct gctctatttg actgagcacc gtcaaatatt gatcgtaagt agcggtggca
     5761 gtcacttgac catcctttaa catcgtatcg aataactgat ctagttgcgt gacttgcgcg
     5821 atctgaatat cagcgaatcc tagatattgc ataacaaccg ttttccaagg actatcttta
     5881 aaaaagctca tattttcacg tacacgagcg acatctgcac ttgtcacctt ttctttgatg
     5941 atgacatcgt ctacagcttg ctgccagttt gatacatttt tagtaaataa atcgttgaca
     6001 gctgtctgga tcttcagttt tgattctgca ctatctaact gtgtcacgat tgctttcttc
     6061 tcatcttgga ttgcaacagc ttctttaggc aattctgatt ctttgatgtc gtaatcatct
     6121 gctgaaagtt tgacagagga tagcttattt gacaaacttg tgattgtttc ttctgttatt
     6181 tcagggttta gaaaaatttt ttcttttgta ctatacagct tgtctacttc ttttgataaa
     6241 gaagtcaagg tgtctttttg attggtgatg gcagattctg cataattgat ccgttgctga
     6301 tgttgccgct gattattgat atggtatcca aacaaacaga tcgcaatagc acctgcacct
     6361 accgccacag cagtcaatga cttgacgaat ggcttttcca tagattcact cctaagtatt
     6421 gatgatagat aaattaagat ataattcact tacataaaaa tgacagcttt ttatttaaaa
     6481 aaacataatt aaactaatat attcacagaa atatttgtaa acaagttttc cctatataat
     6541 cataccacaa actgttttct tgacta
//
LOCUS       DAZUKR010000135         6475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000135 DAZUKR010000000
VERSION     DAZUKR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.137"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..3070
                     /locus_tag="ACG89C_002403"
     CDS             <1..3070
                     /locus_tag="ACG89C_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4900904.1"
                     /translation="SSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLADNED
                     LATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVKNLLG
                     NNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNIFPSD
                     KTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHPGGAA
                     MVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADGIGN
                     ITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDSLSEK
                     SDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGATWNKN
                     LLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGSFIA
                     RVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVGGIG
                     KGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEEYVQ
                     MPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLLDDE
                     KRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGAVNA
                     NGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYTAAA
                     NYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMGSGDI
                     FNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGNDGLA
                     EGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGKGHG
                     YANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKSEINS
                     VDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            3101..3946
                     /locus_tag="ACG89C_002404"
     CDS             3101..3946
                     /locus_tag="ACG89C_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFS4900905.1"
                     /translation="MNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESIEL
                     SEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTEID
                     SQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYTKNVWEKKGV
                     FKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFINMKDTQILE
                     VSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGHANVEIELYE
                     ERDLF"
     gene            3943..4992
                     /locus_tag="ACG89C_002405"
     CDS             3943..4992
                     /locus_tag="ACG89C_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900906.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            4996..5820
                     /locus_tag="ACG89C_002406"
     CDS             4996..5820
                     /locus_tag="ACG89C_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900907.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            5838..>6475
                     /locus_tag="ACG89C_002407"
     CDS             5838..>6475
                     /locus_tag="ACG89C_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4900908.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         2311 a          993 c         1296 g         1875 t
ORIGIN      
        1 atcatctaat gcaaagtttc tttcggctaa taatactaaa tttacagagg ttcttttatc
       61 atctgacaga atgtggaatc aatgggtaaa ccttcctgat aatgagaaaa gattacttgc
      121 agataacgaa gatttagcta ccactgtttt atcttcagaa gaaatatacg gaagatggac
      181 tcaattacca gataatcaaa aatatatgtt agctgacaat actgacttag caaacaaaat
      241 attcgcttca gaagaatcat ggaatgcatg gaaagcaatt ccagacacag ttaaaaactt
      301 gcttggtaat aatattgatt tactggctaa agtgaaagac gggactgtaa gtgtagaaga
      361 atataacaaa gttatgccac ttcttaaaac tctatatgga gataacgtta atttaaaaga
      421 ggcaatcagt ggggctaaaa gctctataga gaattacaac aaaaatattt ttcctagtga
      481 taaaactgca gtaggacacg atgaggcttc acaagctgct aaagatgcat ttgaagcgtt
      541 caacatcttc caaacaaaaa taccagataa aattactaaa actgtatcag ctgactttat
      601 aggtcctatg ccgaaaaatg cgaaaggaac aaacttccat cctggcggag cagctatggt
      661 aaacgatcaa aaagggccta cttatgaaga gcttataacc ttgccaagcg gagaaacttt
      721 tatacctaaa ggacgtaatg tcgttttaga cttaccaaga ggttcgaaag tactgaacgc
      781 cactaaaact aaacgtctag tgcctaaata tgctgatggt ataggaaaca taacgacggt
      841 ttcatctacg ctaaacttag atgctttaat tttagctatt aacgagctaa cgacggtatt
      901 gagaactcaa ctgatggcag gaacgactga tgctaaagat actaaactat cagatgaaac
      961 aaaagttgcc gatccgatta ttccggacag tctttctgaa aaatcagatc agtatctagg
     1021 aattggagct caatggttaa ctaatctaat gaatggttgg aactcagcgg ttcctcaata
     1081 tatgaatagt gaaatggttt ttattacaaa ctatctcaat gcgctaaaac ttcaaaacaa
     1141 tcctaattat acgcaaggag caacatggaa taagaactta ctgaatggat ggaatagttt
     1201 aactgggacc tttattgcta caatcaattc attttgtaat caagcaatgg tcacgcttag
     1261 aaactacaac actcctatgt acaacaatgg ccgaacgtgg cagcaaaata atcttaatgg
     1321 ttggaattca ttgtatggat cattcatagc ccgtgtaaat cagcttggta acgattcgat
     1381 taacaacctt cgttctaaaa gtggtggttt ttacaatgca gggacattct tgttgcaatc
     1441 tttaataaac ggaatgaatt cgttaggtaa ttctctttct actacaatga acaatgtcgc
     1501 taatacaatg gttggtggta taggcaaagg agttaatggc gtcatatcgg gagttaacta
     1561 tgtgcttaag gaagtcgaat caagtaagaa tatcggaaac tggccaattc cacaatacgc
     1621 taagggaact gatggacatc ctggtggact tgctatgatt aatgatcaaa aaggcctagt
     1681 tcacgaagaa tatgttcaga tgccagatgg acgtagtttt attgccaaag gtagagatct
     1741 tcttgttaat ttgcctaaag gtgctcaggt cttaaatgct tctttaacaa agaaattaaa
     1801 ggaacgttta aatattccac actacgaaaa aggtattggc aatttagata ttgttgattt
     1861 gttagatgat gaaaaaagaa tgttagaatt cttaactagc aaagttgatt tttcaggcat
     1921 taatgaacca tggcttgata tgactaaatc aggaacgtca ttaatgtcca aagctgcaaa
     1981 tacaatgctt caatcaaaat taagtgaatt cttcactcat ggcaattttg atggtgcagt
     2041 aaatgccaat ggtgtttatc aatatttagt tgatgtggca cagaaagtaa tgggtaagtt
     2101 ccccggactt acagtaactt ctggatatcg agcaggagat gcctactatc acggaaaacg
     2161 gcaagctatc gacttggctt atccaggtat ttctggagat ccgagatata cagcagcagc
     2221 aaattacgct tttgaaaaat tcccttcaaa aattgcgtat gtcattacga atggacgtgt
     2281 acgtgaccgc atgggattat ctggaacaag gtcaagcgga caatggacga attggccaga
     2341 tggcgatcac ttcgatcata ttcatttaaa cggttcgatg gggtctggcg atatattcaa
     2401 tggaggagtt ggaggaagtg gtgttgcacg ctggcgttct tatgtgtcta aagcattgaa
     2461 aatgaatgga ttaccagcta cagcagcgta tataaatgcc tggatgtccc aaatccaaac
     2521 ggaatctggt ggtaatgaga aagcaatcgg cggcaatgat gggctcgccg aaggcaatgc
     2581 aacaggatta cttcaaacga aacccggaac ttttgcagct aacgcttttc ctggtcacgg
     2641 aaatattatg aacggatttg ataacatgtt agcagcaatc aattatgcta agaaacgtta
     2701 cggagttgca ggtatgttgc aagttatcgg gaaaggacat ggatatgcca atggcggctt
     2761 gattaccaaa gacggactat atcgagcagg agaaggaaat aaaccagaaa tggttattcc
     2821 attgacaaga aaaacaagag ctatcgaact tatgggtcaa gcattagcct tcctttctgg
     2881 aagcaataaa aaagaaacga aaagtgagat taattctgta gataatagac atatcctaca
     2941 agcgatagaa aaacagtctc gtatgacaaa tgatctgcta tcattattac tctcgttttt
     3001 taaaagtaac aatcgatcag ataaagaact agctttagat attcagaaaa ttttaatcag
     3061 gaggacatag tatttgagga cagtaatatt aagaaataaa ttgaatgaag aaattgattt
     3121 gtccacagaa gattattttg ctactgaaat gtcgaatcta ggttttgaag ttacaaaaga
     3181 gcatattgga caatggggga gtttcagaga aataggagag agtattgaat tgtctgaatt
     3241 tcaatcatca gttatgattt ctgttcacgg atttagagaa caacaattat atggttcatt
     3301 agttgaattt ctggctaatg gaccttttga actagaattt tcttttgatt cagaaacaat
     3361 gatcaggaaa tgtagtttga aatcattatc taaaacagaa attgattccc aaagctcatt
     3421 gctaacagat actttgaatc tatattttac ttcaagctgg tattctatta agcgtgaaaa
     3481 attaatacag aattcaactt ctctcaatac acgtggaaaa gtttactcat ttgttcgacc
     3541 atatgtctac accaaaaatg tatgggaaaa gaaaggtgta tttaagttca gtaataattc
     3601 tgtatatttg acaaatagta cagagcgaat gtctccgtta aaaataagga ttattggaga
     3661 gtgctcaaat ccatattggg aagttatcca aaactctaaa gtagtagctt cagatggcta
     3721 ttttattaat atgaaagaca cacaaatatt agaagtgtct agtttgctag aagaccaaac
     3781 ggcaatttta aaagatgtag gtggagttga atcttctgtt tatcaacaac aagattttac
     3841 taaatcaaac ttcgtccaag cgcctctagg agaattcagc gttgtttttc atgttggaca
     3901 tgcaaatgta gagattgaac tttatgagga gcgtgatcta ttttgatttt agccattacc
     3961 ctctttcacc gagatatgaa tttatataac gaacgtttaa tttcgactga gttcgaattt
     4021 ggaacggatg aaattggaga agaagtaagt aattttatta cagataaata tgtccatgta
     4081 aaatcaggag attttctact agcaaaattt attcctagcg ggaagattgc ttattttgga
     4141 gtgattagct cgcaagaaga caaaaaaata aattgcaaag gattactcag tttggccgat
     4201 agcgagatac cgactgtaaa aatatcaggt gctaattatg aagagcatat tcgacgactg
     4261 attgaatact acttattgaa cgatccaaca aaacaactaa aaaatatttt ggaggtaaaa
     4321 acagaaagcg ttacttctca ttcttatcaa gctgttgaca caaataagag acgattgaat
     4381 gcatatataa taaatgggtt caaaaaatat aatgttaaat ggtatgtcaa agagattaaa
     4441 ggtaaaaaaa tatatactgg tattcgagca gttgatgatt caatttatat taaagataat
     4501 tcatctgagt ttaatgactg ggatgttttt gtgcaatctc ctggaccagg gaacgagaac
     4561 aaattattga tcgtagataa agcaatgaaa gatatagaaa atcccttggt attgtctacg
     4621 tggtatttag atgacgaaaa taatcttacg caagattcaa cgaataagaa cattgcattg
     4681 cctaccgtta atttagtaaa tatatatgat caaactcaag aagatagacc ttcttatgaa
     4741 gaggtagcta aatcggagct taaaggcaac gcctattcac atgaaattaa agttaatgtt
     4801 gtgctaaatg ctagaaatat caatgtagaa aatatcgaaa caggaatgtt agcgactatt
     4861 gtttacaaag agcagactta caaatctgtt ctaactgcct ggcgaatatc cagcagtaaa
     4921 aagaatatag aattgacatt tggtaatgtc cgaagtcgtt ttatggatta ttttgaagat
     4981 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
     5041 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
     5101 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
     5161 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
     5221 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
     5281 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
     5341 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
     5401 ctatgctcag ttaattctac tggatcaact gtttcaatct caaagatatc ggatagttac
     5461 gagctgactc tctctaaagg agaaattttg tggaggggga ctgcgttaat gcatgatact
     5521 caaactgtcc aaccttcaaa aaaaatttgg cagacagttt caggtttttt gttagtatgg
     5581 ctcccatacg aaaacgggca agcaattgag gatagatatg taactacgcc tttttataag
     5641 gagcgtataa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
     5701 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
     5761 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
     5821 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
     5881 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
     5941 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
     6001 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
     6061 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
     6121 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
     6181 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
     6241 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
     6301 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
     6361 aatcagataa gcaagtaaat gatcgtattg ataaggcaaa taatcgtatt gatcaaacca
     6421 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc attga
//
LOCUS       DAZUKR010000136         6374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000136 DAZUKR010000000
VERSION     DAZUKR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.138"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..383
                     /locus_tag="ACG89C_002408"
     CDS             <1..383
                     /locus_tag="ACG89C_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900909.1"
                     /translation="DSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKEFGDLS
                     GEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAISIKD
                     LKELLNSKRNELKQIKETNSVELE"
     gene            397..1086
                     /locus_tag="ACG89C_002409"
     CDS             397..1086
                     /locus_tag="ACG89C_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900910.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1211..3562
                     /locus_tag="ACG89C_002410"
     CDS             1211..3562
                     /locus_tag="ACG89C_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS4900911.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3642..4037
                     /locus_tag="ACG89C_002411"
     CDS             3642..4037
                     /locus_tag="ACG89C_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900912.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4362..4979
                     /locus_tag="ACG89C_002412"
     CDS             4362..4979
                     /locus_tag="ACG89C_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4900913.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5020..5319
                     /locus_tag="ACG89C_002413"
     CDS             5020..5319
                     /locus_tag="ACG89C_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS4900914.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5353..5520
                     /locus_tag="ACG89C_002414"
     CDS             5353..5520
                     /locus_tag="ACG89C_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4900915.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5565..6170)
                     /locus_tag="ACG89C_002415"
     CDS             complement(5565..6170)
                     /locus_tag="ACG89C_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4900916.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2199 a          886 c         1259 g         2030 t
ORIGIN      
        1 tagattctgt taattcaaac gctcaaataa aagatcaaag agtagctgaa cataataata
       61 aaaaaatgag tttgaccaaa aatatctatt ctatgtcaga tgaagaattg tataaagaat
      121 ttggtgattt atccggggaa cagttagaag aaagtattcc aggaattagt gactcagata
      181 aaattgttgt cttgtctgct ggtgggcaat taatgggaga tggcaaatcc gaattagtta
      241 gttataaaca aaatcacaga cagtttattg atgttaatac tgattccaac gctatttcga
      301 ttaaggatct aaaagagctt ttaaatagta aaagaaatga attaaagcag ataaaagaaa
      361 ctaattcagt ggaattagag taaaggaaga ttcattatga aaaagatttt tggtgtttct
      421 gttgggctac ttatgatgtt aacatttgga aatgcagtat atgctgctga gaaagaagtt
      481 atttcagatc cagagcagta cgcctcacga tactttgctg attttgaaga agaagataaa
      541 gtacttgcat tgccagaagg tggtttttta aaaggggaag ctaaaattgt atctatggat
      601 aatccagata taattcaagg ttcatataat tcagaaactg atcctgcttc agtttcagtg
      661 acagaagcaa aaaaaattgt tgctgaaaaa ttcaaaaatg atattgaaac tactccaatt
      721 atgcctcgag gagcatttcc acccgatgtt ggtactagcg gacaccttct tgtactagca
      781 ggaggaggaa gctataaatc acagccattt agtggtgcgg gatggcgttt tggaggtatg
      841 ggtgtaaaac cagctgatgg tacaggcaac tttttacgtt ggaaatccta cattgacgat
      901 ggaagagtag gaaatgtaaa tcaagccaac gcaactaaat ctggttctat tcaaggaatg
      961 gcattgtatg ctcgtgaagg attcaaatac tttgcaccaa acgggcctgg ttggacaatg
     1021 aatataggta tttattacac ttataatcct gttgcaggta cgtattatgt tgttcaaaat
     1081 atttaataaa acagcacaaa gagatttctt tgtgctgttt tattattttt agttgttgac
     1141 aaacactaaa atataaaata taatcaacac ataaacgaac gtatgttcgg caagaaagca
     1201 ggggattttt atggatgaga tggttttaag tactcagaaa tggctaaaca agaaatacag
     1261 taatgttaca gggtttgata aggttccaga aaatgggcga acagggtggc ctacaatcta
     1321 tggacttatt gaaggactac aagtagaact tggtattact aatctagtag ctaattttgg
     1381 acctaccact gaaaaaatgt acgataatca ggttactcct aaatggggca aaaatctacc
     1441 taaaaatatt gtatttctta ttcaaggtgc cttttggtgt aaggggatta atccaggtgg
     1501 cttcgatggg gtatatactc catatttaga tactgctgta aaagagttgc agactgatgc
     1561 agggtttaat ggaagcgctg taactggagt tttggatgcg aaatgggcca aagcgttatt
     1621 tgatatgagt gcttttgttc ttgttcccgg cggagatagt aaaattagaa aaatgcagca
     1681 atggttaaat gtaaattatt tagaatacac gggaataatg ccttgtgatg gtatttatca
     1741 aagaaattca aatcaagcat taatttttgc attacaagct gaactaggat attctcccag
     1801 tgaagcaaca gggtcttatg gtaatgggac aactagtaaa acttatcctg tgtctgaagg
     1861 taactcggga aactatgtaa gaataattca atatggatta tatgttaatg gttattttga
     1921 agacggtaca ttcgatggta ttttcactaa atatatgggt ttagaagtac tggcattccg
     1981 aaaatttatg gtattgcctg aatatactga aatagcagat gttgttgtaa taaaaggact
     2041 attgtctagc gctggagata ttagaagatc tgcagatggc gcagatactt ctacgcaatt
     2101 aactagatct caaatccaaa cattggttga taatgatata aaaatagttg gtagatatct
     2161 tactggtact gttggaattg gaaaagatga acgaaataag tatctaacta cagaggaatt
     2221 gaataatatt ttttctaaaa atttatctgt gtttcctatc tatcaagatg gaggtgcagc
     2281 attagcttat tttacttatg atcgtggctt atcagatggt aagaaggcta tagatgcagc
     2341 aaaaaatcta aatataccat taacaacagt tatttatttt gctgttgatc tagacatgtt
     2401 aggggaagaa attcttgcat atgctggaga atattttaag ggggtatctg ctgtaatgtc
     2461 atatagcggg tatcaaacgg gtgtttacgg aacaagaaat gtttgttctc aaataatcaa
     2521 taacgggtat gcttcgtttg catttgtttc agatatgtcg actggttata gtgggaattt
     2581 aggttttcct atgcctagac aatggtcttt tgatcaattt gttgaattta ccataggaag
     2641 tggcaacgga gctgttggta tcgatttaat cgctacatcg ggtagagata gcggatttaa
     2701 tgagttatca aatgatacta ataatgataa ttacattgcc aaatacaatc agaaggttat
     2761 aaatcagatg gtacgtgcgt atcaatatct atcacaagct ggtcttgata atccttggaa
     2821 ccctcatttg acattttata gatatgtaaa ttattctggt ttgtcatggg acatcatttc
     2881 ttctcctgta acagagcatg atagaaaaat ttatgatgaa tataggaata aattaactaa
     2941 tagcgagggc ctgtataatt attttataga tcctaatact ggttcaataa taggtttgcc
     3001 tcacttaatt gtgacgttac aatcacagat gtttctagcc gacacaatca atgatagtgt
     3061 ttctgacttt gcaggttggt taggagattt aatgacttgc tggggtgaag ttaagaaatt
     3121 aggaatacaa atggagcaag gtgtatttgc attagtaggt acggctgcag gtggaacatt
     3181 tagtctagaa gatttatacc aagatgttga cgcggtaaat gtttatacct tagcaaaaag
     3241 aaattctgat tcaacagctt tatcggcttt aaaaacgtac tattgtgata agggtgctat
     3301 tgatagattt aatcaatttg ttaagaacag atttggttct tcagataaaa tcgcttcagt
     3361 aacaaatagt ttattagaaa atgacgaaac agttgatatc ttaggtgtag ggataatttc
     3421 atttttcatt gataaaatag ttaataattc acttgggaca gattttactt atcttgattt
     3481 tattggagat gaggggaaag ttgcaaatgg attttctgaa aaaattattc aatttcaaga
     3541 aaatagaagt aaacttaatt aagaatcgtt tactatttaa acagtgtcct tttccttttt
     3601 aaatatatac gataaaataa gtatatgaat tgtaaaagga catgatgatt atggaagaaa
     3661 tagaatggaa taatctgact caaaataata aaaataattc tggatgtgga attatcttta
     3721 tgtttgttat agttttggga atagtagtac ttttaataac tgtcaagata gtagattact
     3781 ttgcgaatac tcctgaagaa caaatacaga gaaaacttcc tgagtttgaa agagtaccac
     3841 cttatgatcc aagagataat tcatttcaag taaaaaaatt aaaagagtct ccaaatgaaa
     3901 tgtggtttga agttacagtt gaatcaagcg acttcaaaga aaaaaagaaa gaatacaatt
     3961 atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa ccctaaatta gaaatatata
     4021 gtgttgtaat agaataatag tgttaaaaaa attgtaaaag ttaaagaagt caaattctga
     4081 tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa actggataac tagtttattt
     4141 aatggcagac cgttaaggcc tgccattttt atgtatttta gcggaataaa tataatcatg
     4201 cgggagtgta aacttactgt ttcaaaaagt atactttttg acatgccttg attttctttg
     4261 tttatgtatg gcataataac cacataatac aatttgaaac gtttctgatt taatctgatt
     4321 gttttgaaac aattttagtg aaaaaagggt agataaaaac gatgagaaaa tttttttata
     4381 tccgggtcag tagttatgat caatcgatcg aacgacaact gatttccgcg aaggaattag
     4441 gaatccctga agaatatata tttgtagaaa aagccagcgg caaagatttt aaacgccctg
     4501 aatatcaatt aatgaaacgg atgtttcgaa aaggagattt actctatcta caatcattag
     4561 atagactagg cagaaataaa caaatgattt tagaagaatg gcgagaatta actaaggaaa
     4621 agcagattga tatttctgtg ttagacatgc ctctgttaga tactgctagt tacaagcaac
     4681 tagatgggtt agagacgctt atctctgact taattctgca attactcagc tatatggccg
     4741 aagacgagcg gaaacgaatt aatagccggc aaagagaagg cgtagaagct gcgaaacaaa
     4801 agggtgtaaa attcggcaga aagaaaattg ctttacctcc tgaatttcct gcgatctatt
     4861 ctgactggaa agcaggaaag ataactgcag tctatgctat ggatcaattg ggcatgaaac
     4921 gaaatacctt ttatcgacgg gtgaaagaat atgaacatac cttgcaccac ttttcttgat
     4981 agatataaca gctattagac gggaagggag agtttgaaaa tggacttaac gaatcaaagg
     5041 gtcgttttta ctggaacgct gcagcaaatg acacgaaccc aagccatcgg tttggttcac
     5101 tctttaaacg gccattgtca atcaagtgtt acttcaaaaa caaatttcct ggtttgtggt
     5161 cagtattcta agtcgttgtt tgatgattct ctctatacaa aaaaggaaca aaccgcgcgg
     5221 gacttgattg cgttaggtca tgagattacc attttatcag aagtcgaatt ttatgcgtta
     5281 attagtcaac agttgaaaga atggcaacgg tatttataat cgttagtaaa atttacaagg
     5341 aggaaaattt ttatgaagcg aaaatcttat gatcgttggg gcattgaaat aactagtttt
     5401 aatcgaaata ttgcttttca cgtggagtgt gactgtgggg aattagcaaa aagtgtttat
     5461 caaaaaaatt acttttactg tcctaaatgc catcgccgat acggggtaca tggaacatag
     5521 aaacgaaata gtgcggtaga aaagcgaatg aatgtagtat atttctacaa atcattcgct
     5581 ttctttattt tataaatagt tggtcgagag atcccgtttt cttttgcaat ctctgcaact
     5641 ggaatgcctt gctttaacat gtccaccacg gttttaaaaa ctaagcgttt ttgcggatct
     5701 gttgagttaa tagaatactc gattggtcgt cccttatatc ggccggcttt ttgagccaat
     5761 aatactcctt gccgctgacg ttcacgaatt ttctcacgct cgttttgtgc aatataactt
     5821 agcagtgata aaaacatatc gaacatagcg gtatccagga gttcatttcc agtattaaaa
     5881 tctaaaaact gtgcatctaa tatttttaac gctacttttt tctgtttcat aaaagcaact
     5941 gtatttttta tatcttcata atctcttcct aaacgatcta gtgatgtaac gatgacgcaa
     6001 tcaccttcac ggatataatt tagtaatttt tgaaactcag gacgctcagt atctttacca
     6061 ctggcttttt cttggaataa tttatcaatg ttaagtcctt ccatcattgc caattgtcga
     6121 tccaaatttt gatcttgtga agatactcga atatagccaa tttttcccat aaaaaactcc
     6181 tttttaaaag tgatataatt tacatgtaaa atttacttgt agttttactg ctatcatagc
     6241 atttttaaaa ataaatttta tgtagactta aaatttactt catggagggt ttaaatggat
     6301 gaaatcaaaa gtaacaaaaa agcatttggc atttatttag gagaacctag tacagaacaa
     6361 ttaaaccaat tttt
//
LOCUS       DAZUKR010000137         6363 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000137 DAZUKR010000000
VERSION     DAZUKR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.139"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..196)
                     /locus_tag="ACG89C_002416"
     CDS             complement(<1..196)
                     /locus_tag="ACG89C_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS4900917.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITR"
     gene            420..1220
                     /locus_tag="ACG89C_002417"
     CDS             420..1220
                     /locus_tag="ACG89C_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4900918.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1239..3107
                     /locus_tag="ACG89C_002418"
     CDS             1239..3107
                     /locus_tag="ACG89C_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4900919.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3100..3591
                     /locus_tag="ACG89C_002419"
     CDS             3100..3591
                     /locus_tag="ACG89C_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4900920.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3579..4133
                     /locus_tag="ACG89C_002420"
     CDS             3579..4133
                     /locus_tag="ACG89C_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4900921.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4151..5146
                     /locus_tag="ACG89C_002421"
     CDS             4151..5146
                     /locus_tag="ACG89C_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4900922.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(5288..>6363)
                     /locus_tag="ACG89C_002422"
     CDS             complement(5288..>6363)
                     /locus_tag="ACG89C_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4900923.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         2143 a         1030 c         1300 g         1890 t
ORIGIN      
        1 ttctagtaat cattgggaaa aatttcaagc agtagatcgc acaaatacct acgacgatat
       61 aaatgatctt cgcaaaaata gttgttgaac caccagcaat catagctact aaatcaagtt
      121 cgaacaagcc taccaacagc cagttcaacc ctcccacaat caataaagtt aatgcaatag
      181 catctaaagt tttcattaga attctccttt cttaaatgat aaaacaatta tatatcaaaa
      241 agagaaacat tgcttatata acgctcagga attttagcaa tctatgcaaa aagatgaaaa
      301 ttttttttgc tcttccatta gtacacattt tttagctgaa gctgtaaatt tcatgtctta
      361 atttttttta gtgagctttt atactggatt tgtaagaaaa aagaaggaga ggatcacata
      421 tgtcggattg gttggatatt gcaggaaaag tagtaattgt aactggaggt tcctcaggaa
      481 ttggaagaag tatcgtcgaa aatttgttga agcagaatgc acaagtagcg aattttgatg
      541 taactgaatg ccgtatacag catgaaaacc tactatcttt aaaagtcgat gtgtcttcta
      601 aaacggacat aaaagaaggg atatataaag taatgaaaca ttttaagacg atcgatggtc
      661 tagtcaataa tgcaggaata aatattcctt ctcttctaat agatagaaat catccaaaaa
      721 gcaagtatga attatcagaa caagtttttg ataagatgat cgctgtcaat caaaaaagtg
      781 tatatttgat gagccaagca gttggaagaa tcttggtgca aaagggatca ggagtgatcg
      841 tgaatctttc ttctgaatca ggattggaag gatcagaagg acagagctgt tatgctgcta
      901 caaaagcagc gatgaacagt tttactcgtt cttgggcaaa agagttagga aagaggaatg
      961 ttcgtgtggt aggtgttgca ccagggatac tggaagaaac cggtttgaga acacaagaat
     1021 atgaagaagc tctttcttat acgagaggta taagcgtcga acagctgaga aacggttaca
     1081 gtcgaacaag tacgatccct ctaggaagga gtggtaagct gcaagaagtg gcggatctag
     1141 tctgctatta tctttcagaa agatcaagtt atatcactgg agtcacgact aatatttcag
     1201 gaggtaaaac aagaggataa aggatagggg ggacgcacgt gacgctagta aacagatggt
     1261 accaattatt gaaattacta gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag
     1321 aaatcaaaac cagtaatcaa accataaaaa aagatattgg ggagttgaat gaagaaattg
     1381 ccggtattgc aaaaatcatc caaaaaaaca aacattttga acttgaaatt tatgattttg
     1441 atttttttga tgagatcatg caaggaaaat tgaaaacagc cagtgatttc aattcggcat
     1501 ccaaaagaat cgcttattta ctaaataggc tgatcgaaac agaggatttc ttacgtatgg
     1561 atgatttgtc tgaagaagtc ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat
     1621 taaaagagat gatcaatgat tttgatgtaa agattacagg cacaccaaat aaaggactaa
     1681 gaataatagg agatgagcta gaattacgac tacttttatt ttattttgtt tctccctact
     1741 tttcagagac gaaaacagaa cagtttctag aagaaaaaca agcaatcaaa cattttcaga
     1801 agaagacgaa tgctagtaaa caagaaatca cccttttaac gaaagttatc ggtatttctt
     1861 tgaatagaat ctctagtaac tatctgttga agaagcccat caatcgttac agtgggtgtt
     1921 ttgaatatat ccaagaattc aatgaactga tcaatcagtt ggaagaaatc tatgaactaa
     1981 ctttgagcca atatgaaaaa gattttatca gttttccttt gaacatcatc aacacaagta
     2041 taacgatgat aaggaaagct aactctgatt tgcgcttata tttcgacccc atgatgatgc
     2101 gtattcgacg attagatctc attgatctgg atgagtcctt cttatatcaa gagatgaaac
     2161 agcacctctc tttattgata aatcgggtaa tttttcgtta tcgattgact gatttatttt
     2221 atggtgagat agaaaaaaag tatccttttt cttaccaaat cgctagcgaa tgcatacaag
     2281 cattagaagc tatcttgcaa cataagatct ctaaagtaga gatcagctac ctaacgttgt
     2341 atttggagtt agcatttcga tatcaaaaag agcatttttc ctctaaaaaa gtagccattg
     2401 ttcatacgac aggaaaagga acagcattaa tgatccaaag acaaatcaaa aggatattgg
     2461 ggagggatat acaaataaca catgtttcag aagaacaata tgaaaaagta gatctaaaca
     2521 aatatttcgc tgtttttaca acgattccgt taaaaaatgt aaatgaagag acaccggtga
     2581 tccatttgac taatcttttt gatgactctt ggttaagggc acaatggcaa aaagcaaaaa
     2641 aaaagcgatt agccgggttt cattctactg tttttttcta tcaaatatgg aaagaaaaaa
     2701 agacgtatca agaatattta aacaaattag tcgctccttt gatcagaggc tattatgtgg
     2761 atgaagaatt tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca tttgttaaca
     2821 aagaatttgc ttttcctcac acaaccaacc agctatctca acacattgtt ttatcagtag
     2881 ggctaataac tgatcaaggt ttagcagaaa caggaggaaa tggagttatt tttttatttg
     2941 ccatacctga agaaatgacg gctgaagttg agaatgaatt atttgagcta tacgatatcc
     3001 tatttgaact cataaaaatg ggaatagatg agtttattga attagcaccg acacaagaat
     3061 catttttaaa actaatcaaa agtaaaggag agagacttca tgaatgatat gtatctattc
     3121 atagttttcg cagcttcagc ttatgtgctg caagcagtta tcagttggaa gcaactaaag
     3181 catttcaatc aaacatatag cgaactgcga agaaaaggaa aagtagcgat tggtaaacga
     3241 cctgggaaaa tccgttcagg cacgattgtt atgctagcta tagacgatca tgatcaagtt
     3301 ttagaagtca gaaaaatgca aggggttaca tctttggcaa aatttcattc attacctgtc
     3361 tatgcgggtg aagatatcca ttacatcgat cgttaccatc cctcagttcg agcggaagat
     3421 cgtttgacac aagcagcttt agaaaatgcc agagaagtat acttacgaat agaggcagga
     3481 aatgaaaaaa gtgaacaacc ttcattgcct tttattgcta tgctcaataa ttttcggata
     3541 tggaaaagta cgatcaaaga aaaaaggagt gacaaacgat ggatatcata acaaaatttg
     3601 ctgagggatt tatgaggctg ttccaaacag gtgcagaaac attcataagc tggatgacag
     3661 gaatcgtacc agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt gcttttttag
     3721 gtgaagaccg agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt actcgttacc
     3781 ttgtgttacc gttcttatct gcttttatgt tgggaaatcc tatgtcattt acaatggctc
     3841 gtttccttcc agaatactac aaaccaagct attatgctgc tcaagctcaa ttttgtcata
     3901 cgagcaatgg tgtattcccc catattaatc ctggagaatt gtttgtatgg atggggattg
     3961 ctcaagggat cgaaacctta gggctaaatt cgatggagct ggctatccga tacttgcttg
     4021 tgggactgct gatgaacttt attggtggtt ggataactga ttttactact ggatttgtat
     4081 gcagacagca aggaatcgtt cttagcaaaa aagtagagct ttcagttgat tagagaaagg
     4141 agagttttaa atgtatggtg atattaagat cgtaagagga aacggcgggt atggaggtcc
     4201 tttgattatt aggccgacaa aagataaaca caagttcatc tatgtcacag gtggaggtga
     4261 gaggccaatg atcgtagata aaatagaaga actgacaggt atggagtcta tcaatgggtt
     4321 caaaacatca atacctgata gtgaaatcgc gcttgcaatc gttgactgtg gagggactct
     4381 tagatgtgga atttatccta aaaaagggat accaaccatc aatatcgttc ccacaggaaa
     4441 aagtggccca ttagcacaat atatcactga agatatttat gtttcagcag tagggttaga
     4501 acaaatacag ccactaatgg aaactgatga taacaaagaa acagtaaata ctgcatctcc
     4561 agataaaaag tacaaatatg atacttctaa aaagataacc gagcaaaaag cagagcagca
     4621 aaactggata gcaaaaattg gaatgggcgc gggaaaagta gttgctacat tcaatcaatc
     4681 agcaagagaa gcagtagata cgatgatccg gacagtgatc ccttttatgg ctttcgtttc
     4741 ccttttgatt ggtgtgatac aaggttctgg gatcggtaat gtatttgcta acattatggc
     4801 acctttagca ggaaatgcag ttggattaac aatcattggc tttatttgtt cactgccatt
     4861 tttatctcct ttgcttggtc caggggcagt tattagccaa gtgattggaa cattgatcgg
     4921 tgtagagatt gggaaaggaa atattcctcc ccaattagca ttaccggcac tatttgctat
     4981 caatactcaa aatgcatgtg attttattcc agtcggtcta ggtttggaag aagcaaaagc
     5041 tgagacagta gaagtaggtg taccatcagt tctttattca agatttctta acggcatccc
     5101 acgagttatc attgcttggg tagcaagttt tggattatat agctgagttt agtctaatgg
     5161 tgataagtca agatgaaatt taagattttc tttattgagt aatatgtttt gaagcgcacg
     5221 aaactagacc cgatcaaaat caaatttttt tcgggttgat tcaaggattt gcgtcaataa
     5281 cggattgtta tttctctatc acactccttg gtggcgtata gagttggtgt ttacgtagta
     5341 gcgtatccac cagacgcgta aattttcttg cggttaggac gagtgcacgt ttgtgttgat
     5401 gctttggaac ctccttatat ttactttgat aaaaagcttt gtattcagga agataatttt
     5461 ttacagagtt ggcagcttca actaagtagt atctaaaata tcgatttcct tgttttgtca
     5521 gaggtgtatt ttgcgactga tagtttccag attggttcac tttccaactt aatccagcgt
     5581 attttgccaa ttttgtctga tcttcaaatc tttcgatttg accaatttca gcgattaaac
     5641 cggcagcata gactttacca acgcctggaa tactggttaa gcattgatat tctggaatga
     5701 caacaattac ttgttcgatg gctttatcta attctttgat atttttttca agtgctcgta
     5761 tttctcgtac gagaacactt aaaacaacat taattgattc ttgtgcaaga gcacctaggc
     5821 ggtaactcat ggtaattgca cgttgaattg ctttagcgat tttttctggt tgcttgaaac
     5881 ggccttttcc tttttcttga agtaaatcac agaaagcttc taaaggaagc tcagcaagtt
     5941 catccaatgt gaaatcttcc gtcatgagtg aaatgagggt tgcactaaaa agactcgtcg
     6001 attcgtctcg catttctcga gcaagcgtat tacatttgta cgttagattt tcaagaaaat
     6061 gttgctttgt acgaatcaat tgcttaataa gttgatagcg agtacgtgtt aaacgctgta
     6121 aggccatgta tttctcttct ttgatagggg aagttgtgaa tcgttgaata cgcaagaaat
     6181 cagcgattct taatgcatcg tttcgatcag ttttattctc atcaaacatg cgactaaatt
     6241 gtttcacccg aaaaggattt tcaatcgtta caagtgtgtt gagcttcttt agtttctcat
     6301 cttcatgaaa aaacatggaa ggatggaagc tgtacatgga cgttgactcc atgccaatca
     6361 caa
//
LOCUS       DAZUKR010000138         6333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000138 DAZUKR010000000
VERSION     DAZUKR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.140"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1037..1702)
                     /locus_tag="ACG89C_002423"
     CDS             complement(1037..1702)
                     /locus_tag="ACG89C_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900924.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1828..3864
                     /locus_tag="ACG89C_002424"
     CDS             1828..3864
                     /locus_tag="ACG89C_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS4900925.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3868..4506
                     /locus_tag="ACG89C_002425"
     CDS             3868..4506
                     /locus_tag="ACG89C_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4900926.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4534..4962
                     /locus_tag="ACG89C_002426"
     CDS             4534..4962
                     /locus_tag="ACG89C_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900927.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5419..6330
                     /locus_tag="ACG89C_002427"
     CDS             5419..6330
                     /locus_tag="ACG89C_002427"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4900928.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2181 a          966 c         1176 g         2010 t
ORIGIN      
        1 caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt aaagagccga
       61 gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac agtcttggtg
      121 ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa gatgttgata
      181 aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa gctaatttta
      241 agagattctt attagaagac gtttaggaat ataactaaga aaatgattat acaggtaatt
      301 gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag gccagaatca
      361 gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa tattcccact
      421 aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc ccatgctacc
      481 gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt ctgccattac
      541 tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat gataggcgct
      601 tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc gattatcata
      661 tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat agaaatataa
      721 aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat ctatattact
      781 tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg tgaatgtcca
      841 tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata ttatactata
      901 agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct caaggctttt
      961 tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt tttctatctc
     1021 tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt aaggcgtcgt
     1081 catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa gtgagcaggc
     1141 gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc tctttttcta
     1201 cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta catacccaac
     1261 gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt tgtggtttca
     1321 aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata agaaaaagat
     1381 agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag aagggttgtt
     1441 ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt tctattgatt
     1501 cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga tgtaaaagtg
     1561 acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc gttttatcgg
     1621 aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg cggtaagttc
     1681 gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata tagatttatt
     1741 ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta tgattttatc
     1801 aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac agttttactc
     1861 acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa taaacaggta
     1921 ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat tcgattgcca
     1981 caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga aaatgtctca
     2041 tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga agtagataca
     2101 tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca aggggatgtc
     2161 aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac gtccattgaa
     2221 gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag agaaaaagaa
     2281 gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt tcacactgat
     2341 tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt tatttcaaat
     2401 acagacgtat ataattatcg agtgtattta actattggat tgattctttt attccttttg
     2461 ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa taatgacttt
     2521 tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat tacattcata
     2581 attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt tctttatttg
     2641 ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat ttctctttta
     2701 ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata cattcttttt
     2761 acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt agcaactatc
     2821 gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat cgctggtgag
     2881 gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt tatttattcc
     2941 aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa aaacggaagt
     3001 atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg cagagggata
     3061 aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg aaggttgatc
     3121 caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata tagacataca
     3181 gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag tgaaaaagaa
     3241 ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc ttctatcccc
     3301 tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat gtatttggaa
     3361 agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg ctctttgtcg
     3421 aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt gcagttatca
     3481 tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc atttcattac
     3541 ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta tgtcatgtta
     3601 tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag tggttattcg
     3661 ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg aacgacaagt
     3721 gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat ttttttcttt
     3781 agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa gaaaagtcag
     3841 ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa aggtgcgaaa
     3901 aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga tgctggggat
     3961 cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa tatattagga
     4021 ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac gaaacaatta
     4081 actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt tcaagatttt
     4141 gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa gttttcgaat
     4201 ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt aaatataaac
     4261 aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag aatcgcctgt
     4321 gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac tgggtcattg
     4381 gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc ccaaggtacg
     4441 ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa gaagatcatg
     4501 ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat tatttgtgca
     4561 ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc aaattcagtt
     4621 cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga tgctaaaggt
     4681 aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc aattaatcat
     4741 agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac aggtcattct
     4801 aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg aaactctatt
     4861 cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg aaatagaact
     4921 gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa aagtttatat
     4981 aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag ttttaatata
     5041 ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat gtctggaaaa
     5101 tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa acaaaatcga
     5161 taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga cttgaaaaac
     5221 ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta ttctgctgag
     5281 cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca gattagtaaa
     5341 ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc aaaaaacgaa
     5401 aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt actaggcact
     5461 ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta taaaggcggt
     5521 acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc agattccgct
     5581 gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga cgtatcaacc
     5641 aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc atttgattca
     5701 caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa acaaagtcta
     5761 gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt aaaaacggct
     5821 tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag tgaagatgat
     5881 gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt agccaaagac
     5941 aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga ttccactact
     6001 acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg tcaagtttct
     6061 gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt ggttaaaatg
     6121 gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt gaaagaaatt
     6181 gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg cgaagtattg
     6241 aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag tacattcact
     6301 tcagattcta gtacagcaag tagtaaataa gcg
//
LOCUS       DAZUKR010000139         6303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000139 DAZUKR010000000
VERSION     DAZUKR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.141"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACG89C_002428"
     CDS             334..714
                     /locus_tag="ACG89C_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4900929.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACG89C_002429"
     CDS             696..1610
                     /locus_tag="ACG89C_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4900930.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACG89C_002430"
     CDS             1855..2544
                     /locus_tag="ACG89C_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900931.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACG89C_002431"
     CDS             complement(2665..2751)
                     /locus_tag="ACG89C_002431"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4900932.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACG89C_002432"
     CDS             3427..4377
                     /locus_tag="ACG89C_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4900933.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACG89C_002433"
     CDS             4398..4931
                     /locus_tag="ACG89C_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4900934.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACG89C_002434"
     CDS             4947..5117
                     /locus_tag="ACG89C_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900935.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACG89C_002435"
     CDS             5567..6100
                     /locus_tag="ACG89C_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900936.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACG89C_002436"
     CDS             6112..>6303
                     /locus_tag="ACG89C_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900937.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAZUKR010000140         6289 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000140 DAZUKR010000000
VERSION     DAZUKR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.142"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..1533)
                     /locus_tag="ACG89C_002437"
     CDS             complement(55..1533)
                     /locus_tag="ACG89C_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900938.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1831..2766)
                     /gene="murB"
                     /locus_tag="ACG89C_002438"
     CDS             complement(1831..2766)
                     /gene="murB"
                     /locus_tag="ACG89C_002438"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4900939.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2839..3759)
                     /locus_tag="ACG89C_002439"
     CDS             complement(2839..3759)
                     /locus_tag="ACG89C_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4900940.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3872..4624
                     /locus_tag="ACG89C_002440"
     CDS             3872..4624
                     /locus_tag="ACG89C_002440"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4900941.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5488..6021
                     /locus_tag="ACG89C_002441"
     CDS             5488..6021
                     /locus_tag="ACG89C_002441"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4900942.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1900 a         1201 c         1047 g         2141 t
ORIGIN      
        1 aaaaaacata aatcagctaa taaatatgtg agacaaagct gccatccata aatactagct
       61 gattttttca gttaaatgtc tatttttttc tctgatgatg gtatttccaa aatattccca
      121 gtcatctgga gtcggtggat ttttccaaat tatctgttct ttattaaatt tttcaagtaa
      181 acaatcagat aaataaatat tcgtacgaga agtgattcga ctctcaatca caatattgta
      241 atttttaaat cctttcactt gagaaataat gtaatcggta taagcagatc cttgcgaaaa
      301 atcaatacag acatgaatcg gtgcttcaaa atatgatacc ggacaaatac cgaccaataa
      361 aaagagataa tcataaaata agcgaatcat gaaacgttca tcttttccca agaaatagcg
      421 tttattctct tgtatgacct tttggattgc tgtactgaat attggatacg tctcgaagaa
      481 aaactgtagc tgctttttag acgtaaagct agtcatctca aagtcgaata tccaattttt
      541 tcgattgaca tgcatcatac ccatacgaag ttcttttcgt atatatagtc tttcttcttc
      601 agagatttca atcgaaaatg gtaattgatc aatcaattcc actgacattt tttgactaat
      661 catgtggata tcatgaaaat gagcttccgc aagtccaaat gcgccatcta attcaaattc
      721 ttcttccgtg atcaaaaaca acatgatata agaaatctca tcaatgatct gctctttagg
      781 gatatgaaga aaagaaatat gtgataaatc cttgatgatc ttcattcctt ctgagctctt
      841 aaagtattca tctgaaaaga aacgttttcc gattggttga ttcccatagg ccctcaacaa
      901 actaatagtg gttagcaaat tgaatttgat tcgttttgtt gcagacagtt ttaaattgaa
      961 cagatattca aaatattgca cgatactctt tgcttcattc atgacgcttt ctctaaatgg
     1021 catagaaaaa ccattaaaga ccccgaaata taccccaaat aatacgttcc gtatgtttat
     1081 ttcttcccca actaatatgt ttttctttaa ctttatttga tgctgttcaa gaaaagagac
     1141 taattctctt ctttttacat aggctcttga tatgcttaaa aaatgattct ctgcaaattt
     1201 ttcgatcgtt acttttgaat ccacgatctc cgttaacagt cgagcaattt ttgatcgttg
     1261 aaagtagatt cttcttaaat ttttgatatc cgtcaaattc agacgttcga catggatctc
     1321 tgcttcttct gaaatttgga tctctggctt tgttttgaat tcagtaaatt ctttatttaa
     1381 ttctatgata taatttcttg ttttaaattt agacaaacct aatgtttcat ataacttagg
     1441 cagcactaca taattctttt tctcaaattc taaatatcta gctagcagca gtttttctct
     1501 atcttcctct tctataaggt aatccaactt catatcctac acctcaaaaa atgcctcata
     1561 ccaattactt gatatgcaga tgaaagttca tcttttcacc tatactgatt ttatgatgga
     1621 ttatgcttgt tttttcatca gttattctca ttttttatct tttttttgag aatattgcaa
     1681 aaaatggaaa aatttatgag gaaatattct atattttttg ttttattgtg ttaaaatgta
     1741 caagatttgc gccatttgcg taaacaacca caaaaaagag caggacggcc accgtcctgc
     1801 tcttttactt attttaagtt ccattactta ttaattggct aaacttctgc ttgtttctga
     1861 tattgcttgt ttttctccaa tgatccgcac ttctgtttct aaagacacat caaatttttc
     1921 tttgattact tcttggatgt gcgcaatcaa ttcaatataa tctgtcgctg tcgcatgatc
     1981 gatattgacg atgaatccag catgtttttc agagacttgt gcaccgcccc atttcaggcc
     2041 ttgcaatcct gcttgttgga tcagctgacc tgtatagtgt cctactgggc gcttaaagac
     2101 acttccgcat gaagggtatt ctaatggctg ttttgattga cgcaattcgg tcaactcatc
     2161 cattcttgct ttgattgctt cataatcccc tgtttgcaaa ctgaatcgag cttccaatac
     2221 gatagcacgt aattcttgga ctttactgtg acgataagaa aataccattt cgtcttttgt
     2281 caaagttttc aatgttccat cttcaagcaa aagatccact tcagcaaaaa catctttgat
     2341 ctctccgcca tacgcacctg cattcatata aaccgctcca ccgacacttc ccgggatgcc
     2401 gcaagcaaat tcaaatccag tcaatgattc tgccaaagct gcataagtcg tatcaatcag
     2461 tgaggcacct gcttccgcaa tgaccatcgt tcctttgaca tgaatctgtt tcatttcagt
     2521 cagcatgatg actacaccgc gaatcccgcc atcttgaacg atcagattgc ttgcatttcc
     2581 taataccatc caaggtatat catggattcg gcaataatcg atgatttgct ttacttcttc
     2641 ttgttttttc gggaatgcaa ggacatcagc tggtccacct gttttcgtaa acgtatagtt
     2701 catcaatggt tcatcaaaca atatagtaat cgtggctaag ctttcaacta tttcttcttt
     2761 attcactgag gattttcctt tcttttctca aacgtcctca ttattctatc acgcttaata
     2821 caatcttgcc agtcttgttc aaagttgatg acggtagtga tctttaagca cttcctcaca
     2881 taattcatga cttcttacaa cttctttttg tttaagcaat tctgtttccg atttacctaa
     2941 acggatctga gaaaagaagg cctctaccat tggctcaaac ccgcgtttga ctagcgtgtt
     3001 tgtccagtcg ccaaattctt tcactttttt tccatcagcc tcttgaatga caagctgggt
     3061 cagatcgttt acttcataag taccattttt acttgttact tgatattttt ctatattagc
     3121 gccacttgct aaatccatcg ttaaaatagc cgtttgattt tgtgtttcta attgtaaaat
     3181 ggcatattcc aaaaattcct ttgtttctct tatctggtat tttgttctca aaatgggttc
     3241 atctaataaa tacacagctg tatcaataag atgtaaaaac aaatcataaa ggacgaactc
     3301 tgtcgtttct tttgccgcta ttcgattttt ttgcaggtgg atcaaccgct tatctgggat
     3361 acgtttcaat tcttctacca taggagcaaa gcgccggttg aaaccgatca tcaaaattat
     3421 ttggttctta tcagctaatt cttgcaattc acgaacttca tttagatgga cactcagtgg
     3481 tttgtcaata taaacatgga taccattttc tagacatttt ttggctaatt cataatgtac
     3541 ttttgttgct gcgtggatca tacatgcttc gattttttct tctaaaagct catcgatcgt
     3601 ttcatataga tgttggaacc cgtactcttt ttgaattctt tcttttgttt ctttatttct
     3661 tgttgcaaaa taaaaatcaa gtgttccttg ttttttcgca taagttggta aatatgcttt
     3721 ttctgcgata ttccctacac cgattacacc aatcttcatc tactttcctc ctggatcatt
     3781 tagttacttt catcttacac cattttttac taaaatgagg tacaatagta tcgcaaaggt
     3841 aaggaaatta attcgaagga gtgtgctttt attgaaattt atctcttgga acgtaaacgg
     3901 gcttcgtgcc atcgtaaata aaaatttttt agaagttttt catgagttag acgccgattt
     3961 tttctgtctg caggaaacga aactccaagc aggacaaatt gatttagatc ttccaggtta
     4021 ttatcaatat tggaattacg cggaacgaaa aggctattcc ggcacagcga tttttgctaa
     4081 aaaaccagca ttgaatgcca cttacggaat gggtatcgat atccatgata cagaaggccg
     4141 tctgattact ttggaatatt ccgatttctt tcttgtaacc tgttacacgc caaattctca
     4201 aagcgaatta aaacgtttag attaccgatt agaatgggaa gaagcctttt ataattattt
     4261 ggaaaatcta aaaaaacaaa aaccagtcat cgtgtgtggg gatctaaatg tcgctcatca
     4321 aaagattgat ttgaaaaact ggaaaacgaa tcaaaaaaat gctggattca ctccagaaga
     4381 aagagcggct ctttctcgac tgttggataa cgggtttatc gatacattcc gttattttta
     4441 tccaacccag gaaggtgtct actcttggtg gaactaccga ttcaattcaa ggaaaaacaa
     4501 tgctggttgg cggatcgatt actttttgac gagcaaagat ttagagccag gactagctga
     4561 tgcaaagatc catacggaca ttatgggcag tgatcattgt ccagtagaat tagatttgaa
     4621 ataaagaggt tgtgaaaaag tcgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
     4681 gctttccaca ttttgcataa atttggcata ataccgagaa actggctttt gaacactgtt
     4741 tatcgagaga ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     4801 agttcctcat atttttcgtt tggttgggct tattaccgca gatacaagct tttgaacacc
     4861 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     4921 aatagttcct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
     4981 accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac
     5041 aaaaatagtt cctcatattt ttcgtttggt tggacttatt accgcagatg caagcttttg
     5101 aacaccgttt attgagaagt tgtgaaatca gcgtcctgac ctgagtatta gagaaacaaa
     5161 tggcaaaata gttcctcata ttttgctatt ttttgagcta ataccgaggg tcagctgatt
     5221 gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt
     5281 ttttgaaaat ggttcctcac aactttgcat aaatttgact taataccgag aagtcatgat
     5341 ccccatctaa ctccaaatca caaggaatga gttcaaaaac tagcttttct caacaaatat
     5401 tacataaaaa attctatgtt ttttgttaaa tttatttgct tttttcaaaa gtatgttaca
     5461 gtaatttccc aaggaggttt tacagaaatg aattttgttg ccatggattt tgaaactgcc
     5521 aatcatcagc cttatagtgc ttgttctctt gcgctagtca tggtaaaaaa tagccagatc
     5581 gttgatgaat tctatacatt gatacagcct gagactcctt ttttctggcg aaatgtccaa
     5641 attcacggaa tccatcaaga agacgtacgc aatgcgccga aattcccaga agtctggcaa
     5701 actatccaac catattttca aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat
     5761 acaaaggtct tagcgggatg cttagattat tatcatcttg aacagcctaa ttatctttct
     5821 ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac
     5881 actgtgtgcg aaaatttgaa catccaatta gatcatcacc atgatgcatt ggaagatagt
     5941 cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa
     6001 aaacttgtga ctataaagta aaagaatact tatgaaacac acaaagaggt cgtgagcctg
     6061 ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata gcttctcatg tttattaaat
     6121 attgtgactg gtgaccgagg agctggcagg agaacaccgt ttattggaga ggttgtgaaa
     6181 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     6241 tttggttgga cttattaccg cagatgcaag cttttggaca ccgtttacc
//
LOCUS       DAZUKR010000141         6287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000141 DAZUKR010000000
VERSION     DAZUKR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.143"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACG89C_002442"
     CDS             190..690
                     /locus_tag="ACG89C_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4900943.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACG89C_002443"
     CDS             787..2634
                     /locus_tag="ACG89C_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4900944.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACG89C_002444"
     CDS             2637..3533
                     /locus_tag="ACG89C_002444"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4900945.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACG89C_002445"
     CDS             3583..3972
                     /locus_tag="ACG89C_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4900946.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACG89C_002446"
     CDS             3959..>6287
                     /locus_tag="ACG89C_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4900947.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAZUKR010000142         6176 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000142 DAZUKR010000000
VERSION     DAZUKR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.144"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..283)
                     /gene="rpmB"
                     /locus_tag="ACG89C_002447"
     CDS             complement(95..283)
                     /gene="rpmB"
                     /locus_tag="ACG89C_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4900948.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            553..915
                     /locus_tag="ACG89C_002448"
     CDS             553..915
                     /locus_tag="ACG89C_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4900949.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            967..2649
                     /locus_tag="ACG89C_002449"
     CDS             967..2649
                     /locus_tag="ACG89C_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS4900950.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2766..4802
                     /gene="recG"
                     /locus_tag="ACG89C_002450"
     CDS             2766..4802
                     /gene="recG"
                     /locus_tag="ACG89C_002450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4900951.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4851..5852
                     /gene="plsX"
                     /locus_tag="ACG89C_002451"
     CDS             4851..5852
                     /gene="plsX"
                     /locus_tag="ACG89C_002451"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4900952.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5974..>6176
                     /locus_tag="ACG89C_002452"
     CDS             5974..>6176
                     /locus_tag="ACG89C_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4900953.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETV"
BASE COUNT         2058 a         1076 c         1413 g         1629 t
ORIGIN      
        1 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
       61 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
      121 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
      181 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
      241 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
      301 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
      361 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
      421 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
      481 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
      541 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
      601 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
      661 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
      721 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
      781 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
      841 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
      901 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
      961 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
     1021 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
     1081 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
     1141 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
     1201 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
     1261 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
     1321 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
     1381 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
     1441 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
     1501 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
     1561 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
     1621 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
     1681 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
     1741 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
     1801 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
     1861 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
     1921 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
     1981 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
     2041 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
     2101 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
     2161 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
     2221 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
     2281 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
     2341 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
     2401 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
     2461 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
     2521 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
     2581 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
     2641 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
     2701 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
     2761 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
     2821 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
     2881 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
     2941 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
     3001 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
     3061 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
     3121 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
     3181 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
     3241 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
     3301 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
     3361 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
     3421 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
     3481 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
     3541 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
     3601 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
     3661 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
     3721 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
     3781 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
     3841 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
     3901 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
     3961 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
     4021 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
     4081 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
     4141 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
     4201 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
     4261 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
     4321 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
     4381 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
     4441 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
     4501 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
     4561 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
     4621 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
     4681 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
     4741 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
     4801 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
     4861 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
     4921 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
     4981 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
     5041 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
     5101 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
     5161 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
     5221 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
     5281 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
     5341 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
     5401 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
     5461 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
     5521 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
     5581 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
     5641 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
     5701 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
     5761 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
     5821 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
     5881 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
     5941 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
     6001 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
     6061 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
     6121 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagt
//
LOCUS       DAZUKR010000143         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000143 DAZUKR010000000
VERSION     DAZUKR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.145"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG89C_002453"
     CDS             725..811
                     /locus_tag="ACG89C_002453"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4900954.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG89C_002454"
     CDS             complement(932..1621)
                     /locus_tag="ACG89C_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900955.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACG89C_002455"
     CDS             complement(1866..2780)
                     /locus_tag="ACG89C_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4900956.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG89C_002456"
     CDS             complement(2762..3142)
                     /locus_tag="ACG89C_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4900957.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG89C_002457"
     CDS             complement(3592..3879)
                     /locus_tag="ACG89C_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4900958.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG89C_002458"
     CDS             complement(3899..4123)
                     /locus_tag="ACG89C_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4900959.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG89C_002459"
     CDS             complement(4578..4748)
                     /locus_tag="ACG89C_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900960.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG89C_002460"
     CDS             complement(4763..5296)
                     /locus_tag="ACG89C_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4900961.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG89C_002461"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG89C_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4900962.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1154 c         1041 g         2345 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 accggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtacatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZUKR010000144         6001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000144 DAZUKR010000000
VERSION     DAZUKR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.146"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG89C_002462"
     CDS             <1..395
                     /locus_tag="ACG89C_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4900963.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACG89C_002463"
     CDS             398..1228
                     /locus_tag="ACG89C_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS4900964.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACG89C_002464"
     CDS             1244..2095
                     /locus_tag="ACG89C_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4900965.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACG89C_002465"
     CDS             2092..2451
                     /locus_tag="ACG89C_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS4900966.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACG89C_002466"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACG89C_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACG89C_002467"
     CDS             2624..2929
                     /locus_tag="ACG89C_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900967.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACG89C_002468"
     CDS             2929..3240
                     /locus_tag="ACG89C_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4900968.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACG89C_002469"
     CDS             3237..3410
                     /locus_tag="ACG89C_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900969.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACG89C_002470"
     CDS             3407..3823
                     /locus_tag="ACG89C_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4900970.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACG89C_002471"
     CDS             3823..4491
                     /locus_tag="ACG89C_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4900971.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACG89C_002472"
     CDS             4488..4865
                     /locus_tag="ACG89C_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900972.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACG89C_002473"
     CDS             4862..5161
                     /locus_tag="ACG89C_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900973.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACG89C_002474"
     CDS             5151..5309
                     /locus_tag="ACG89C_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900974.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACG89C_002475"
     CDS             5403..5819
                     /locus_tag="ACG89C_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4900975.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DAZUKR010000145         5843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000145 DAZUKR010000000
VERSION     DAZUKR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.147"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(298..1080)
                     /locus_tag="ACG89C_002476"
     CDS             complement(298..1080)
                     /locus_tag="ACG89C_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900976.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1145..1981)
                     /locus_tag="ACG89C_002477"
     CDS             complement(1145..1981)
                     /locus_tag="ACG89C_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4900977.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1999..3408)
                     /locus_tag="ACG89C_002478"
     CDS             complement(1999..3408)
                     /locus_tag="ACG89C_002478"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4900978.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3422..5245)
                     /locus_tag="ACG89C_002479"
     CDS             complement(3422..5245)
                     /locus_tag="ACG89C_002479"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4900979.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1798 a         1265 c          951 g         1829 t
ORIGIN      
        1 atatgaaaca attacatatg attttttcat aaaaaaacag tactgtctag tagtataaaa
       61 tatacaaatt tcttaaaaat tttttcacta aaatcactac ctgaaacagt ctaaaacact
      121 tatatatcaa cgtttaatga aactatctcc gtttcatttc agatacaaat aaatatagtc
      181 gggctccaaa tccgaaaaaa aataccccca gtagcttatc ctaagctact ggagatgtat
      241 aagtacggtc atataacaac ggggtagggt aaataaatga aaaagatgga agaatcatta
      301 ttcaataact tttttaaata cttcgttgta atcagttacc tgtccgttct taaataccac
      361 agaataatct tttttcccat tttcaagtaa ccgaagtgga atgtctttac caacttgctc
      421 tgtcactgca ttactgatac ctgttagatt agctaataaa tctgataaag attgagcatc
      481 tgtagaaatc tctgtatcag tatttatcgt gattccttta tcttcattta caagcttcca
      541 agtaggtggt atagctgtgt atgtttcgat gaatgaatca attctttcat ctatcgtctt
      601 cccagtatct acttctgtct cattactctt agatgaatcg attctttcat ccatcgtctg
      661 ctccgtaact gcttctgtct cattattctc accggaaact ctcttcacac ccaaacttat
      721 atattgtcca tctttagtgt gcatagttaa aattcccacg cttttactaa taaattcact
      781 ataaccagtt ctttcaatag agacttcaat gtatttcaca ctatcatact caaaagaaaa
      841 atagacatgt aaaagcattt tgttgtttac atctacataa taatcctttg actctacttg
      901 agaccttcct atacggaata cagctttact gtctgatatc tctataccag catccttatt
      961 attaatctgc caatctccaa gtagctcttc tggcaagcta gtagtttctt gtgaacactt
     1021 taccacttca ctggtagagg tcgaaacatt cacctccgca ctgttagaag aattttccaa
     1081 ggaatcagga gccttagcac aaccaacaag tctttatata ttgtgcagcc gatattttct
     1141 tcattcagta cacccttgaa atatgcagtg ttagataaag cagttcgtcc tgatttgctg
     1201 aggcacccat ctcttcttga acgtactcct tgattttcaa ggccgcccga tactctttcg
     1261 gaaaacgctg cttcactgaa gccagcaaaa aatcatcctt ctccggcaac tcttccttct
     1321 taatcattcg ggcaataaaa aactgtaaat gcgtcacaaa tcttaagtaa agcatcgaat
     1381 ctttatcaac ctttttttcg ggaaacagat atcctagcat agccaagata tcctttagtg
     1441 ctcttaccga gagcatggtt tcttccatat ttgcttgttc actttgggca tttacaatgt
     1501 gaaacgcaat gtttccagcc tcctcttctg gcaactgaac attcattttt ttgttgatca
     1561 attggatagc atagcagccg atctcaaatt cttttgggta atacttttga atttcaatca
     1621 gcaggcgatt ttgaaaaaca atgccttttt gactgcgttc aatggcatgc ttcaagtgat
     1681 caaccaaggt aaaaaataga cttttattca gcttaaagtc aaacttggcg ttggcataat
     1741 cgataattcg cttgacgcaa ataattagat catccggagc agcctcaaac agtttaatgt
     1801 aatccttttt tagagcatca gaaagtgaat ctaatttaaa tactttttgg attttgtcag
     1861 ccggaaccaa atcgcctttc tttagttgaa atccaatccc tttccccatc aaaattactt
     1921 ctttgccttg cttatctaag caaagaatta aactgttgtt taacgtttta attactctca
     1981 ttagattcac cgttctctct aagctaagcc atttcttgcg attacttttt gataccaaaa
     2041 gaagctttct ttttttattc gttttaattc tttaagattc tcatcggttc ggtcaacata
     2101 tataaaacca taacgcttat tgaagccttg atgggtacta acaacatcaa tcgctgacca
     2161 aggtaaataa ccaaaaacat ctacaccatc tgttaaagct gcttgaatag ccagaatatg
     2221 tttggctaga taatcaattc gatcctggtc attaattcgt ctatcttcct caactatgtc
     2281 gggtgcccca tagccatttt cagtaattaa aataggtaaa tcatagcgat cgtacaattt
     2341 cctcaatgtt aatcgtagtc cagtagggtc aactacccag ccatatggcg ttttatctac
     2401 ataaggattt tcagctgccc ggtagacacc ttcttctccc aacatgatct gttgatcccc
     2461 cgctctagcg gaaacatctg atccgtcatt tttgctggca gcgatggtgg cagttgaata
     2521 ataattaatc gcaataaaat cgggtttacc ggaggataaa attgcctgat cctctgcctc
     2581 aattttagga tataatccac gatcattcaa atagttttca aacaaataat tgtattttcc
     2641 ccgaactgct acatctaaga aagaccagcc tctcaatgtt tcccaattat gggctgcaat
     2701 tgcatcttct ggactggagg tagcttgata catagccgtt aaattgattg ctggaccgat
     2761 ttttccttta agattcaact gatgatacaa gtgaattact ctagcttgag ctaataaaac
     2821 atggtgatta atttgaaaga gttctttttt tgaaggcagc tctccacttg gtggcaaacc
     2881 aatcgctcca ggatgaagga tcatcgtgtt ttgctcatta attgtcagcc aatagttcac
     2941 cttgtcacca tactcagtaa gcaatacctg tacatagtcc acaaaataat cgatagttgc
     3001 tcgatttaac caaccgccct gctcctctag aaaataaggt aaatcgaaat ggtacatggt
     3061 gacaatcggt tcgattccct gattcaggca ttcatcaatt acttgatgat aaaaagaaat
     3121 tcccttttga ttggttttat tattttcaat atccataatt cgcgtccaag cgatggaaaa
     3181 gcgaaaagct tttaagccta attctttaaa cagctgtata tcttctttat agtgatggta
     3241 aaaatccgag gcaacttcaa aattagctac cccttctgta tgctgatact tgtcaataat
     3301 agacggttgt ttcccatcct cctgataggc accctctgcc tggtaggcac taatggaggc
     3361 accccataaa aacttctctg gaaatgctga aagctgtttg tattgcacaa aaatcctctc
     3421 cttaactaat ttgcatcaat ggttcgtgtt cctttaagct tccgcttgct tcactaattc
     3481 ctgcgtaatt ttcactgttg gtcactacca caatcactgt atcatcgtag ccagcgtttc
     3541 taatggcagc gaaatcggcg ttgattaata attgtcctgc tgacacctga tctcctgctt
     3601 ttacaaatac ttcaaagggt tcccctttta aattcacggt gtcgatcccc acatggatca
     3661 ataattcaat accttcttta gaacgtagac caatcgcatg tttagattct aaaatagtaa
     3721 tgacttctcc ctcaacaggt gaataaatgc tgttttcttc tggatgaatg gctacgccct
     3781 ttcccaatag tcccttagaa aagacagcat cgttcacttc agaaatagga cacacggttc
     3841 cttttaccgg attttcaaca atgactgctt cggctattgc cgtttgctct gattcatcag
     3901 aagtatcgtc aaatccaatt agataagcaa tgataaaggt tgcaacaaag gcgattacac
     3961 acgtaataat tgcgtgaaca atattcattg gattttctcc aataaaggct ggtaaggctg
     4021 ctaagccagg ggaaacaaag gcatatctga ctaaaccgct caagccagca tacaaaccag
     4081 caattgcacc accaatcatg gaagcaacta atggtttttt cagcttcaag gttactccat
     4141 agagtgaagg ctcagtaatt cccattaaag cagtgatccc agtggacaca gctagctgct
     4201 tcaactcttt gtttttagtt ttcgcggcta cagcaaaggt agcacctgat tgagcaatat
     4261 tagaagccag cattcctggc ccattgatca tttcagaacc cgtactgctc aattgcatag
     4321 tagcgattgg cgtcattgcc caagctgtac ccgtaaccac caaaaatggc tgcaaaccac
     4381 ccatcaaaaa tggaattaac caagctgctt tgccgttaat ccattcagcc cccattgcta
     4441 taagatcgtt cagcaacgtt ccaaacggtc caatgacaac caacgctaaa ggccccataa
     4501 ttaaaaatac cagcatcggc ttagtaaaga atttaataaa cgaaggcgaa attcgatccg
     4561 caaagggctc tacataggac atgatccaaa cagtaaagat aatcggtaaa actgatcccg
     4621 cataatctgc agctaatacc ggaattccta gaaaagaaac ggcatcgcca gtagaaaaaa
     4681 gctgtgaaat atttggatga agcaaaatgc cagcaacagt aatcgccaaa attgggttga
     4741 catcaaactt tatggctgcc ccataggcta atagtactgg cataaaataa aaggctgcat
     4801 cagcaatagt attcaaaatg atgtaagttt gactactgtc tgatagtaca ccagtcaaag
     4861 tcaagacggc caaaatggct tttaccatcc cggcacctgt tatagctgga atcatcggtg
     4921 taaaggtggt agaaatgaca ctaatcaatc ttgaaataat actttcttga ttgtctgaag
     4981 aggggccctt tttaattgaa gttcccactt ccttttgaat ttccttgaag gctgtttgga
     5041 cttcattgcc aatcaccact tggtattgtc caccgctatc caccacacta attacaccaa
     5101 gtgttttttc taaggctttc gtatcggctt tccttttatc tttgagttca aaacgcagtc
     5161 tggtggcaca atgggttaag ctgaccacat tttcttttcc cccaacctgt tgcaatacat
     5221 ttcttcctac ttctctgtaa tccattattc tctctcctta aagcagtaaa aaagaccaag
     5281 tcaaaccacg ccccaatcaa cattgaagtg ttagtttgtc ttggtcttgc ctgcataatc
     5341 agtaacatgc caataacgtt attttgctgt aatccaataa tagcacatta tgatagcgca
     5401 atcaacaggg aatttaattt tttatctttt cgtttgaccg tatagggtaa tataacagtt
     5461 agatcacatc aactagggta tatctgcgta tacttactgc taaaataagt atatctaata
     5521 ttaactatac cagtaaaacc attcctacat aaccagcggg aaattcccaa aatcgtgaaa
     5581 gcatcaaaaa tgcatataac ggatcacaca acttcaacaa ttctgctctt gggaaaaatt
     5641 gatgaatttg gtgagtaaag gatggaataa acagcattcc aacaatatag gcaacaatag
     5701 tgatacttgt ttttttggta aaaatcagta agcaatagcc aatttcaatc attccgagat
     5761 ataaaggtat cgatagaatg atttgggcta gtattcttaa aaattaacta gcaaatgttt
     5821 aaactgattt atttctaatc tac
//
LOCUS       DAZUKR010000146         5790 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000146 DAZUKR010000000
VERSION     DAZUKR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.148"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG89C_002480"
     CDS             complement(99..1229)
                     /locus_tag="ACG89C_002480"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4900980.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACG89C_002481"
     CDS             complement(1877..3079)
                     /locus_tag="ACG89C_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4900981.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACG89C_002482"
     CDS             complement(3139..3342)
                     /locus_tag="ACG89C_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4900982.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4551)
                     /locus_tag="ACG89C_002483"
     CDS             complement(3484..4551)
                     /locus_tag="ACG89C_002483"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4900983.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACG89C_002484"
     CDS             complement(4609..4716)
                     /locus_tag="ACG89C_002484"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900984.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACG89C_002485"
     CDS             4879..5691
                     /locus_tag="ACG89C_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4900985.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAZUKR010000147         5614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000147 DAZUKR010000000
VERSION     DAZUKR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.149"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..2008)
                     /locus_tag="ACG89C_002486"
     CDS             complement(59..2008)
                     /locus_tag="ACG89C_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4900986.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="ACG89C_002487"
     CDS             complement(2050..3108)
                     /locus_tag="ACG89C_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS4900987.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="ACG89C_002488"
     CDS             complement(3227..3682)
                     /locus_tag="ACG89C_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4900988.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="ACG89C_002489"
     CDS             complement(4266..5069)
                     /locus_tag="ACG89C_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4900989.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DAZUKR010000148         5602 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000148 DAZUKR010000000
VERSION     DAZUKR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.151"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..1540
                     /locus_tag="ACG89C_002490"
     CDS             122..1540
                     /locus_tag="ACG89C_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4900990.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1611..3080
                     /locus_tag="ACG89C_002491"
     CDS             1611..3080
                     /locus_tag="ACG89C_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4900991.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            3090..3557
                     /locus_tag="ACG89C_002492"
     CDS             3090..3557
                     /locus_tag="ACG89C_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4900992.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3593..4465
                     /gene="scrK"
                     /locus_tag="ACG89C_002493"
     CDS             3593..4465
                     /gene="scrK"
                     /locus_tag="ACG89C_002493"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4900993.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            4546..>5602
                     /locus_tag="ACG89C_002494"
     CDS             4546..>5602
                     /locus_tag="ACG89C_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4900994.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSE"
BASE COUNT         1885 a          797 c         1139 g         1781 t
ORIGIN      
        1 gttaaatcgg cgaaaaattc cttcgtcgat ttaaaaatat ttttatgaaa cgggttgaca
       61 tatgaaacgg gttgacatat attagttgtg aagataaaaa ccttttcact ggaggaagaa
      121 aatggagaaa ttattaatta aaagcaataa tcatcaactt tactaccaac ctaaggatgt
      181 ttgggtcgga gatgttatgc cttacgggaa agaaggaaaa ttctttattt atcatcaacg
      241 tgatactaga aatccagttc cttttggaga accgtttggc tgggctttag caactacgaa
      301 agattttgtt cattttgaag actttgggga atctctttta agaggggatg acaatgagag
      361 agaccagttt atctacgctg gttctgtatt tgaggcaaat ggaaaggttc acgcatttta
      421 tacagggtat aatcgtgaat tcttagcgca gggaaagaca tctcaagtgt tgcttcatgc
      481 tgagagtgat gattttgttc attgggaaaa atcagaaaca gcattggagt tagaaccaca
      541 agaagggtat gatattcgaa actggagaga tccatttgtc gtttgggatg atgaaaaaga
      601 agaatatttg ctaattttag gggcgcgaaa gggtcaggat aaacatctac aaaccggacg
      661 tttagtggaa tttacatcaa aagatttaac taattgggaa ttcaaaggtg atttttgggc
      721 tccgggattg tataccatgt ttgaaatgcc agaacttttc aagattggtg actggtggta
      781 tcttgtctat tcagaatact cagatggaaa taaaatactc tatcgaatga gtcaaagttt
      841 ggaaggtcct tggattaagc ctaaagatga agcttttgat ggacgggctt attatgcagg
      901 tagaacagct tatgatggaa aacgtcgagt cctatttgga tgggtaccaa caaaagatca
      961 agaaaatgat aaaaacaatt atttatgggg aggtactttg gttcctcatg aagtctatca
     1021 aaaagaggat ttttcacttg gggtaaaacc catagacgaa gtgtggaatg cattcataaa
     1081 tttacatgat attgccgatg tagagataga aacagcagac acaagagaag aagtaacgct
     1141 tgtcaaaaat agtggaactt tgtttaattt agaaacaaca atttcatttg atgagaatct
     1201 tagtgacttt tcactgagat tatataaaaa ccttgagaca gatgaatctt acgaatatcg
     1261 cttcaattta gatgaggggc gtcttacatt tgataaaaac ccttgttacc cttggtttca
     1321 atatatggat aaaggactta ttagacctat atctttaaag cctaaccaat cttataaact
     1381 aaaagtgatt gttgatgaca atataatgac actttatatt gatggaatcg ctcttaacgc
     1441 tcgtgtacat ggttgttttg gtcaagcaat tactattaca gcaacaaatg gatcaatgac
     1501 tgcaataaat accaagtttt caaatgaaat aaagaaataa taaacgtcaa tcatgtggca
     1561 ggtctgaaaa tcaaacatga cctgtcacat ttaaaaggga ggataaatga atggactaca
     1621 aaaaaattgc cgaagatgta ttggaaaacg ttggaggaaa agagaatatc tcaagcgcta
     1681 cacactgcat tactcgttta cgtttaattc ttaaagacaa aagcaaagcg aatatggaaa
     1741 tgcttgagga aatagaaggt gcaaaaggtg ttcttttcaa tagtggacag ttacaagtaa
     1801 ttttcggaac aggtataatt gaaaaggttt acaaagagtt cgttgaattg acaggagcta
     1861 aggaagtatc cgtttctgaa atgaaagacg aaggagtcaa taattcagga aatagacttc
     1921 aacgagcttt taaagttttc tcggatatct ttattccgat cattccagct tttattgggg
     1981 cagcaatggt tttaggaatt cgttcgttga ttaccactcc tggaatgttt ggaatggatg
     2041 gctcattcgc tgatcacaat gtattcctaa atgatttatc caaatttctg ggtattattg
     2101 caaccacatt tgattattta ccagttttag ttatgtattc tgcaacaaaa cgttttggcg
     2161 gaaaccctat tttaggatta cttgtaggat ttgttatgat tcatccagga ctagccaatc
     2221 ggaatgattt cgtcatggga actataaagc ctgattattg gcatttattt ggattaagca
     2281 ttccagcagt tgcgtttcaa gggggtgttt ttccagcaat tttgacttct tggtttttag
     2341 caaaagttga gaaaattaca aataaatatt ctcctcaagt acttgcttac attttagtcc
     2401 caactgtgac aattgttctt gccaactttg cactattttt agtgtttggt ccattgggga
     2461 attttatcgg taatgtttta ggtggaatca ttgatttcct atatatgaag atgggtgcct
     2521 ttggtgcttt tgtttttgca ggcgcgttac aaccattagt tgtaacagga acacaacatg
     2581 ctattcaagg tattgaggca aacttagtcg caacaactgg attcaattat atccaaccta
     2641 tttggtcagt ttcaattatt gcccaaggtg gcggttgtat tggtatgtat ttactcttta
     2701 agaaaaaatc taaagataga gatattgcga tttcaagctt tattccaaca ctggtaggta
     2761 ttactgaacc ggctatcttt gctgttaatt tgaaatattc aatcgttcct tttgtttgct
     2821 cgtttattgg tgctggtttt ggtggagcat ttatgaaaat ttttgatgta aaagccattg
     2881 gacaaggatt gactgtattg ccaggtctga ctattgctaa tccagttctt ccttatatat
     2941 ttggtaatct catagcattc attttaccaa tcatttttat tctcgcttat gataaatata
     3001 aagtgtttat ccctgaagct aaacaccgta aatttgcaga aaaaactgac tcaaaaaaat
     3061 tgccagttca agtaaagtga gatgagagca tgttaaattt ttttagaaaa aatgaaataa
     3121 gcgttgtagc acctgcaacc ggtgtttttg aaaaactgga gaaattaagt gatccagtct
     3181 tttcaaaagg aatgatggga caaggatttg caattgatgc aaaaaacgaa acgattgtat
     3241 ctccaatcga tggttttgtt tcaagtattt ttcctacaaa acatgcctta ggaataaaaa
     3301 caaaatcagg aattgagata cttgttcatg taggaataga tacagttgag ctaaatggtg
     3361 agggatttga tattaaagtt caagaaggac aaaaagttaa ggctggggat attcttatga
     3421 ccattaattt tgtagttttt gaaaagaatc acaagactaa agatgtcatt gttgtttttc
     3481 cagactacca agacgaaatt ttcattccga aacttgaaga agtaaaacaa ggacaagaat
     3541 tgtttgttct tggctaatga taagtaatta ataatagaat cgaggaaaat gtatggtttt
     3601 tggggctatt gaagctggtg gaacaaaatt tgtttgtgcc attggagata aagatttgaa
     3661 catatttgaa aaagtcagct ttcccacgac tacacccaaa gaaacaatga atttagtttt
     3721 agattttttt aatcatcata aagatgattt agaaggtatc ggagtgggat cgttcggtcc
     3781 tattgatatt cattgtgaat ctaaaacgta tggcttcatt acatcaacgc caaaacttgc
     3841 ttggcaggat tttgatttta ttggaagttt aaaaaagcaa cttaatattc caactgcttg
     3901 gacgactgat gtaaatgcct cttgctatgg tgaatatgtt tctggaaatg gcagaggcct
     3961 ttcaagtgtt gtttactata ccgtaggaac gggcatagga ggtggcgcta tccaaaacgg
     4021 aaacttcatt gaaggtttta gtcatccaga aatgggacat atgcttgtaa aacgacacaa
     4081 taaagacaac ttttctggga catgtccatt ccacggagac tgtttagagg gcttagctgc
     4141 tggtccggca atcgaaagaa gatcagggaa aaagggacaa gaagtgactg aaaatgacgt
     4201 tacttgggaa atagaagctt attacattgc tcagtgtgca tataatacaa cattgatgtt
     4261 atcacctgat gtcattatat ttggtggcgg agtaatgaaa caaaaacact taaaaaagaa
     4321 agtacaagat aagtttattg agttgatcaa cggatatgtc gctattcccg atgttgacaa
     4381 ttatattatg acaccaaaat tgttagataa ttctggtatt gtaggttgtt tagctttagc
     4441 aagagacgtt aaagaaaaat cttaatttgg aaaaaagatc gtctcgacta tttgtttggt
     4501 tgatgcgatc tttttttcaa ttagtaattg aagggacgcc taaaagtgaa aaaaaaaatg
     4561 aaagatgaag tattagagta tatcaaaaat aatttaaagt attacgattt ttctgctcaa
     4621 gatattgcaa tgaaattttg tattaaaaga aatgtcgctt cccactattt gaatcagtta
     4681 ttttcagatg gaaaattact aaagaatgat tctgttagac cagtaatgtt caagtataat
     4741 caacaaaagc caaaagactg cttttctaag tttattggag cagacatatc tttgaaatcc
     4801 actatagata aatgcaaagc tactgtaatg tatcccccta atggcctccc attaataata
     4861 aaaggaaaca gtggcgttgg aaaaagtttt ttagcaagtt tgatctatca gtatgctctt
     4921 gatagaaaag taattcataa tgacgcaaaa tttgttgttg tgaattgtgc agattatgca
     4981 aataatccag agctattgtc agcagtttta tttggatata aaaaaggagc ctttactggg
     5041 gctgaaaaag atactgcagg gatgctatct tctgctaatg gaggatacct ttttttagat
     5101 gaggttcaca atctaagtgc agaaaaccaa gagaaattgt ttcttcttat ggattctcaa
     5161 aagtatcgta aacttggtga aagtgtgcaa tgggagtatg caaatgttcg ccttatctta
     5221 gccacaacag aagataaaaa tacctctttg ttagcaactt ttagaagacg catcccagct
     5281 gaaatcactc tgccagatta caataataga agtacatctg aaaaaattca attattattt
     5341 gaatttctta aagatgaagc aattacgatt gattcaaaaa tattttgttc gattgaactt
     5401 tttactgatc ttttatcgaa aacatttgaa ggaaatgttg gagaattgag gaatgaaatc
     5461 aagcttcttt gtgcagaagg ttatttagga aataagagaa agtcttcaat ttatttaggg
     5521 gacaaactag acagcggttt ttggatagat ccagaaatta gtatagattt aaaaaaaatg
     5581 tttcttaaat cactttctga aa
//
LOCUS       DAZUKR010000149         5535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000149 DAZUKR010000000
VERSION     DAZUKR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.152"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..988)
                     /locus_tag="ACG89C_002495"
     CDS             complement(<1..988)
                     /locus_tag="ACG89C_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFS4900995.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACG89C_002496"
     CDS             1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACG89C_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4900996.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ACG89C_002497"
     CDS             1813..2967
                     /locus_tag="ACG89C_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS4900997.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ACG89C_002498"
     CDS             3182..4150
                     /locus_tag="ACG89C_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4900998.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ACG89C_002499"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ACG89C_002499"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4900999.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..>5535
                     /locus_tag="ACG89C_002500"
     CDS             5180..>5535
                     /locus_tag="ACG89C_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFS4901000.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1670 a         1078 c         1252 g         1535 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcgg
//
LOCUS       DAZUKR010000150         5247 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000150 DAZUKR010000000
VERSION     DAZUKR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.153"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="ACG89C_002501"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="ACG89C_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFS4901001.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="ACG89C_002502"
     CDS             1402..2802
                     /locus_tag="ACG89C_002502"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS4901002.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="ACG89C_002503"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="ACG89C_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4800..5247
                     /locus_tag="ACG89C_002504"
                     /pseudo
     CDS             4800..5247
                     /locus_tag="ACG89C_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT         1675 a          850 c         1001 g         1721 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta atatattgaa tcaaaaatat aaattataaa
     4501 ataattagat ggggtatttg gtttaagtat cccttatata gtaataaatt tataggtgtg
     4561 attctcgaat taatacccga aaatgtgaga gaatttttat ggatttttta ggatacaaat
     4621 taattatttt tgcttttatc ctgattacca gtccagtact gtcggtatcc cattttgcag
     4681 caaaatcatg ttgataaagt gacaagaaga ctgatgagat atattatcca aattgagaag
     4741 ataattaatc ggattcttga gtatacccta ctttttaaga aagtagggta tacaagcgta
     4801 tgaataaaga cataactttc cctgtgatgg acgttcggct tgttccaatt accaaaatac
     4861 aggcgaacga ttataatccc aatcgagtag cgtctccgga aatgaactta ttggagcttt
     4921 ctatcattga agatggcttt actcaaccat tagtttgtta ttacaaggaa aaagaagaga
     4981 agtatatctt ggttgatgtg cttcataggt atcgtgttgc taaggaaaga ctcaacttaa
     5041 aagttgtacc ggtaacagtt attgataaac ccatataaga acgtatggct tcaacaattc
     5101 gacacaatcg tgcaagaggg acccatgaaa taagaggaat ggaaattgtc atccaaagat
     5161 tagtaaaaaa tggatggtca gacaagagaa tatccaaaga attaggcatg gacattgaag
     5221 aaatttttcg ctttaagcaa gtgtctg
//
LOCUS       DAZUKR010000151         5235 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000151 DAZUKR010000000
VERSION     DAZUKR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.154"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1451
                     /locus_tag="ACG89C_002505"
     CDS             <1..1451
                     /locus_tag="ACG89C_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4901003.1"
                     /translation="KKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTEM
                     SNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNA
                     ESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFST
                     PQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQ
                     AILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENY
                     EESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELI
                     KTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHA
                     VKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTG
                     NSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1800..2432
                     /locus_tag="ACG89C_002506"
     CDS             1800..2432
                     /locus_tag="ACG89C_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS4901004.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3020..4459
                     /locus_tag="ACG89C_002507"
     CDS             3020..4459
                     /locus_tag="ACG89C_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4901005.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4754..4972
                     /locus_tag="ACG89C_002508"
                     /pseudo
     CDS             4754..4972
                     /locus_tag="ACG89C_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
BASE COUNT         1717 a          847 c         1089 g         1582 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1741 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1801 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1861 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1921 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     1981 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2041 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2101 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2161 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2221 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2281 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2341 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2401 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2461 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2521 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2581 taataattaa cagtgatata taaacatcta taatatatta ttcatactat tgatatttag
     2641 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
     2701 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
     2761 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
     2821 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
     2881 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
     2941 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
     3001 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
     3061 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
     3121 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
     3181 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
     3241 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
     3301 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
     3361 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
     3421 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
     3481 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
     3541 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
     3601 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     3661 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     3721 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     3781 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     3841 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     3901 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     3961 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     4021 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     4081 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     4141 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     4201 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     4261 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     4321 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     4381 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     4441 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     4501 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     4561 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     4621 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     4681 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     4741 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     4801 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     4861 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     4921 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     4981 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     5041 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     5101 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     5161 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     5221 atgaacgctg gaatt
//
LOCUS       DAZUKR010000152         5220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000152 DAZUKR010000000
VERSION     DAZUKR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.155"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACG89C_002509"
     CDS             complement(<1..1256)
                     /locus_tag="ACG89C_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4901006.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="ACG89C_002510"
     CDS             complement(1270..2487)
                     /locus_tag="ACG89C_002510"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4901007.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="ACG89C_002511"
     CDS             complement(2477..4072)
                     /locus_tag="ACG89C_002511"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS4901008.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="ACG89C_002512"
     CDS             4197..4982
                     /locus_tag="ACG89C_002512"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFS4901009.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4975..5217
                     /locus_tag="ACG89C_002513"
     CDS             4975..5217
                     /locus_tag="ACG89C_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901010.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAZUKR010000153         5039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000153 DAZUKR010000000
VERSION     DAZUKR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.156"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            413..619
                     /locus_tag="ACG89C_002514"
     CDS             413..619
                     /locus_tag="ACG89C_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS4901011.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="ACG89C_002515"
     CDS             complement(821..1822)
                     /locus_tag="ACG89C_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4901012.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="ACG89C_002516"
     CDS             complement(1827..3740)
                     /locus_tag="ACG89C_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4901013.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="ACG89C_002517"
     CDS             complement(3908..4414)
                     /locus_tag="ACG89C_002517"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4901014.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="ACG89C_002518"
     CDS             4574..5014
                     /locus_tag="ACG89C_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4901015.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         1391 a         1008 c          769 g         1871 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttc
//
LOCUS       DAZUKR010000154         5039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000154 DAZUKR010000000
VERSION     DAZUKR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.157"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACG89C_002519"
     CDS             complement(139..1338)
                     /locus_tag="ACG89C_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4901016.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACG89C_002520"
     CDS             complement(1468..2844)
                     /locus_tag="ACG89C_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4901017.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="ACG89C_002521"
     CDS             complement(2844..3500)
                     /locus_tag="ACG89C_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4901018.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..4787)
                     /locus_tag="ACG89C_002522"
     CDS             complement(3510..4787)
                     /locus_tag="ACG89C_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4901019.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4806..4967)
                     /locus_tag="ACG89C_002523"
     CDS             complement(4806..4967)
                     /locus_tag="ACG89C_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4901020.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT         1589 a          915 c          623 g         1912 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacctacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg
     3781 aatataaatt gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt
     3841 tcataaattc tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct
     3901 aacataatag aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa
     3961 gactcttcaa acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc
     4021 tctttcaatt tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt
     4081 ttatttgcaa tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct
     4141 acaaacacca tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta
     4201 ccagaaaaga tccctataat tttaaattta tgacttttta tttttccact tttttcaata
     4261 tctagtaatt caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct
     4321 tcatgaacaa taattgaatt cttatcattt tcttctatat ttttaccttc tttaattgta
     4381 aatactccac tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca
     4441 acattcttaa attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact
     4501 ttagcatctt ttagttttgc taatccatca tattgaatta ttttttcttc aatttctttt
     4561 aatttttcaa tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt
     4621 gatattgaag aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt
     4681 attgttaaac aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa
     4741 gttctatttt tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact
     4801 cctctctatt ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt
     4861 ccacatctga tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa
     4921 acttttttac attttccaca attaatacac ttatccttat cctgaatcgg ctacactaga
     4981 ctagacagaa aaaataaggt gtgtagaata ataaaaaaca cactggagga tttttcatg
//
LOCUS       DAZUKR010000155         4973 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000155 DAZUKR010000000
VERSION     DAZUKR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.158"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..1245)
                     /gene="nagA"
                     /locus_tag="ACG89C_002524"
     CDS             complement(97..1245)
                     /gene="nagA"
                     /locus_tag="ACG89C_002524"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4901021.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1321..2157)
                     /locus_tag="ACG89C_002525"
     CDS             complement(1321..2157)
                     /locus_tag="ACG89C_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901022.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2341..3558)
                     /locus_tag="ACG89C_002526"
     CDS             complement(2341..3558)
                     /locus_tag="ACG89C_002526"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4901023.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3707..3868)
                     /locus_tag="ACG89C_002527"
     CDS             complement(3707..3868)
                     /locus_tag="ACG89C_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901024.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3941..4024
                     /locus_tag="ACG89C_002528"
     tRNA            3941..4024
                     /locus_tag="ACG89C_002528"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3975..3977,aa:Leu,seq:gag)
     gene            complement(4264..>4973)
                     /locus_tag="ACG89C_002529"
     CDS             complement(4264..>4973)
                     /locus_tag="ACG89C_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901025.1"
                     /translation="TTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDM
                     NILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAM
                     IWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVI
                     LMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQF
                     GLTVLGICTFIITVINR"
BASE COUNT         1523 a         1026 c          798 g         1626 t
ORIGIN      
        1 acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaacctt aaaaccaaac
       61 ttaagaagtt tagttttaag gttaacttaa attatattat gcgtggaaac gttccacgcc
      121 atctagataa gttgcttcca atgtcatatc agggttcaat acgataaagt ctgcatcgcg
      181 tccttgagcg atttttccac atgtatcatc gattttacag ctgattgctg gaatcagact
      241 agccatcatg actgcttgtt ctggtgttgc taatccccag tctacaacat ttttaattgc
      301 ttctttcaat ttcaatatac ttccagctaa gttgccagat tctagacgtg ctgttccttc
      361 ttttactact actggaaatt cgcctaaatt gtaatttcca tcaggcatcc caccggccat
      421 catacagtct gtaattaatg cgacatgatc atgtcctgct ttttccatta ggattcccgc
      481 agcatttgga tgtacatgat ggccatcaca aatcaattca gaaaagacat gttctaaaga
      541 taataacgca cccaccatac ctggttcgcg atgattcaat ccacgcatac cattgaaagc
      601 atggacaaat acacttgcgc ctgcttcaac agctttttgc gcttcttcta gtgtagcatt
      661 actgtgtcct agagaaacaa ctactccttc ttcagtcact tgtttgacaa actcttctac
      721 accttgacgt tccggtgcta aagcaatttt tttgattaaa ccacctgaag cttcttgcca
      781 ttcatggaaa gtatccaaat caggatcccc gaaataactt gggttttgtg cccctttgtg
      841 ttcttctgtg aagaaaggac cttcaaaata gattcctttg attttagctc ctggtgcttc
      901 ctgatacatt ttgccgattg tctcagctac atcttttaaa cgttctttgc ttgacgtcaa
      961 tgttgttggt aaaaatgaag tcactccaca agataacaga ccttcagaca tcactttgat
     1021 gccttctgca tcattatcca ttacatcatg attcatatag ccgtgaatat gtgtatctac
     1081 caaaccagga gcaatccatt tgccgctttg gtcaatgatg ttgacttctc cctcaggtag
     1141 ttctttggtg tattcaccaa aaagtccatc ggtaatttca agatagcctg catgtttgac
     1201 acctgacgct aaaaagaatt tgtcagcata gataaaagtt ctcattctat tctctccctt
     1261 tgtttttcta cctctaaagt actcttattt agagaagaag tcaagaatgg tctacaccat
     1321 ttacaaattt tttgaaattt gtttcacagc ttgtgctaag ccttcataga atgctgctct
     1381 ttctggttct tctttacgct tgtatattaa gcttttaagc acaaataaat cattgaggta
     1441 atagtagcta ttcttctctt gagcccaata gattccttga ttcacctgtt ctaacgcttc
     1501 gtcgatttca ttttgatgga ataagaaatt tccataatta taaaaaattt gcgaaaattc
     1561 actttttgat cgttcatcag ctgattgatg cagcagttgg atgcttcgac tcaaatagta
     1621 gcgtgcttca gccgtttttc ctgccacacc atatattttt ccgatacaac taatcagttg
     1681 tatttctgtg tcagaaacat ggattctttc tttttggcta gagtagctta atgcttcttt
     1741 caaataataa agggactgag agatattttg acgcaacgtg aattcgcaaa caccgagata
     1801 ataataatag cgttgaaagt cagttgcttg atacagattt tcagaaatat cagaagattt
     1861 tagtacttgt tctaacttcc gataatcacg tttttggaaa caactatcca acagttcaaa
     1921 taatttatcg tttgtccgag tagtttttcg agagagattc atcacatgat caatcgttac
     1981 ccctagccgt tgacaaatct gcatcatcac caatacattc ggcagttcct cattattttc
     2041 gatccgactt aatacacttt gtgaacaaat atcatgcgaa agcatttttt gcgtcatttt
     2101 ttgttctttt cgaatctctt taatgatcgt tccaaaggac tcgattaacg tttccataag
     2161 tatcctcccc aaaatatgcg tattcgcatt ctattcgtat tttactttag gaagtcgcaa
     2221 acttcaagaa aagacttgca actaaataat atactttagt tatttaagaa aactccacta
     2281 gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact gaaaaatttg gtttttaaaa
     2341 tcatttttgg cgataggttg ctaggaattc tctcattttc ttagctgttt gtttcaaatc
     2401 atccatttta ggaagataaa cgattcgaaa atgatctggc tggttccaat taaatccacc
     2461 accatgtaca agtagaatat gatgttcatg taaaaagtcc aaaacgaatt tttcatcatc
     2521 caaaatattg aatcgctttg tatcaatttt tgggaatata taaaaagcag cttttggttt
     2581 tacggctgaa agaccaggaa tatcattgat tgcattatag atatattctc tttgttcata
     2641 gatccttccg cctggcagga gcagctcatc gacactttga taaccgccta gcgcggtttg
     2701 gataatttgc tgagacaata cattggaaca caaacgcatg gaggacaaca tattcagccc
     2761 ttctatatag cctttgacgt gtgacttatc tccacttagg accatccagc ctactcgaaa
     2821 tcctgctaca cgatgagatt tggatagacc gttcagcgta acaacgaaca aatcaggcgc
     2881 taatgtagca atcggaatat gttctagacc atccatcact aagcgatcat agatttcgtc
     2941 ggaataaata atcaggttat tttgtcgagc tacttcaacg atttcttcta ataattcttt
     3001 tggataaagt gccccagtag gattattagg attaatgata acaatagctt ttgtccgact
     3061 cgttattttt gatttgatat catcaatatc aggataccat tctgcctctt catcgcaaat
     3121 ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc gtccacaacg gatagtccgg
     3181 cattggaacc agtacttcat ctccattgtc cagcaacccc tgcatacaca tcgtgatcaa
     3241 ttcgctcact ccgttgccgg tgtagatatc attgatcgtc acattcggaa aatgtttcag
     3301 ttggcaatat tgctcaattg cttttctagc agaaaagatc cccttggaat ctgagtagcc
     3361 ttctgagtca cgggcattca tgatcaaatc acgaatgact tcgtttggtg cttcaaatcc
     3421 aaaaggtgcg ggatttcccg tgttcaattt taaaatacta atgccctctt cttgcatccg
     3481 ctccgcttct tctagaacgg gaccgcgaac atcgtaactg acaccttcta atttgttgga
     3541 tttttcaaat tctctcatca cttagccaat ccctcatttc aaaaagttag aactttccaa
     3601 attttttgtt atttactatt tcaatatcat actccatcct agaaaatttt gccacttatt
     3661 taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt gagaacttaa ttgtcatgat
     3721 agtcattcca tagattcgct gcccaatcca ttgcaattcc acaaatgatt cctagcataa
     3781 aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac acctaagatc attcccagtc
     3841 ctgaaaattc tccgtcatat ttctccatct taataagtcc tcccattttt gagcttgaca
     3901 actatgtaaa acctcgctat cattagtgtg ttattattac gcagatgtgt tggaattggc
     3961 agacaagcat gattgagggt catgtgagcg ttagctcgtg tgggttcgag tcccatcatc
     4021 tgcactacaa ctcgtgaagt taattttgct tcacgagttt ttttgtttga acgtttcttt
     4081 atgccattat atacatatca ctgtatcctt tacaaaaaga acgtacaatc atgtaaacag
     4141 cctgaggtga gtatatgaag gtcggaaaca agctactcgt agaaacttgc aagtgttaaa
     4201 acaattaatc gaaaaacgag taaaatccct cacctaatac taaagcgagg gattttttgt
     4261 atattatctg tttatcaccg ttattataaa ggtacaaata ccaagcacgg ttaatccaaa
     4321 ttgcataaat tcattttttt gtacactcat gcttttcatt agctctttaa ctgccattag
     4381 tactaagatg gcgataacaa cataaagtat atagtacgtc cgcattccgg aagcccatac
     4441 tgtgggagaa aatcccataa ttaaccttga acaaaatccc attatcatca aaataactaa
     4501 aaaaatagaa gttttcttat tgtcaaaact taaataaatt ccaacaatta aacatagtaa
     4561 taatgccaag actactaaag tagctatcca agtacctgga taaaaaagtg acagtttcgt
     4621 tcctaactct gtaaagagat tattcaggtt actactactc caaatcattg cccttttatt
     4681 tccattgacg taagtaaagc tttgacccat tgtattacca aaataaataa tcaggtttaa
     4741 gaaaaacggt aatgcagtca atatacgcac ataatagttt tgacatttcc tatatgttag
     4801 aaggaaaatt aagaaaaata gcaataaaaa taagatattc atgtctaaaa aaaacggctt
     4861 tccaaatgat gaaataccta aatctaactt attaagaaag ttgaaatttt tatattcagg
     4921 aaaccattta ttaatttcat gtgcagcacg taatgcattc cctggagttg tca
//
LOCUS       DAZUKR010000156         4949 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000156 DAZUKR010000000
VERSION     DAZUKR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.159"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..198
                     /locus_tag="ACG89C_002530"
     CDS             <1..198
                     /locus_tag="ACG89C_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901026.1"
                     /translation="FKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSDKKIAY
                     PLLLLLFSFYQVQKLFCFVIC"
     gene            complement(175..1785)
                     /locus_tag="ACG89C_002531"
     CDS             complement(175..1785)
                     /locus_tag="ACG89C_002531"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS4901027.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2007..2252)
                     /locus_tag="ACG89C_002532"
     CDS             complement(2007..2252)
                     /locus_tag="ACG89C_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901028.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2978..4318)
                     /gene="glnA"
                     /locus_tag="ACG89C_002533"
     CDS             complement(2978..4318)
                     /gene="glnA"
                     /locus_tag="ACG89C_002533"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS4901029.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4346..4738)
                     /locus_tag="ACG89C_002534"
     CDS             complement(4346..4738)
                     /locus_tag="ACG89C_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4901030.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1465 a         1060 c          805 g         1619 t
ORIGIN      
        1 ttcaagaagt tttttgcaaa aaataaaata tccaatagcg ctttaatcga aaaaagtctt
       61 ttgctttcta tcttttttcc tacaaataat aacaaatcag aaaaagagaa aagtgacaag
      121 aaaattgctt atccccttct cttgctactt ttctctttct atcaagttca aaaattattt
      181 tgtttcgtga tctgctaatg atgctccgac aaatgctttg attaattttt gcgggcgatt
      241 tggacgagaa atcaattctg ggtggaattg gcatcccaca aagaattttt tctctggcag
      301 ctctacgatt tctactaaac ggttatctgg tgagacacct gaaaagacta gcccttgttc
      361 ttcaaacaat tgacgatatt tgttattgaa ctcatagcgg tgacggtgac gttcttgcac
      421 aacatcggct ccatcatatg cttcagccgt cttcgttcct tttttgattt tacatgggta
      481 taatccaagg cgtaaagttc cacccaaatt ctcgatgttt tcctgatcag ccattaaatc
      541 aataatattg tttgctgtat ctggattcgt ttcagctgat gacgcatctt ctaatccaac
      601 gacgttacgt gcaaattcga cacaagccat ctgcatacca agacaaatac ctaaaaacgg
      661 aacgtcattt tcgcgagcat aacgaatcgc ttcgattttc ccttctactc cgcgatcacc
      721 aaatccacca ggaaccaaga ttccatctgc gtctttcaaa cgttcttcta cattttctct
      781 cgttaattct tgtgctttga tccaatcgat ctcgatgtct gaattatagg cgaaaccaga
      841 atgtttcaat gcttcaacta ctgagatata tgcatctggt aactcgacat atttaccgac
      901 aagcgcaatc cgtacttttt tcttcaagtt caagacacgt tcttctaatt ctttccattc
      961 tgtcatgtct gcttctggtg tatctagttt taaatggtcg caaacaattt gatccattcc
     1021 ttgtgcttgt aaaactaatg ggatggagta aagtgtatct acatctggcg actcgataac
     1081 tgcttctggt tcgacatcac aaaattgtgc caatttgttt ttcacgcttt gagaaacagg
     1141 ttgctctgta cgtaccacca aaatattcgg ttggatacct aaaccacgta gctcttttac
     1201 actgtgctgc gttggtttag ttttcatttc tccagcagct ttcaagtaag gaatcaatgt
     1261 tgtatggata tacataacat tatctgtacc gacatctgct ttcatttgac gaagtgcttc
     1321 aaggaatggc aaagattcta tatcccctac tgttccccct acttctgtaa tgatcacatc
     1381 tgaatcagtc attttagctg ctcgcatgat tttttctttg atctcatttg taatatgagg
     1441 aataacttga acagttgctc ccaagtattc gcccttacgt tctttacgca aaacttcaga
     1501 atagattttt cctgtcgtca cgttggagta tttgttcaaa ttgatgtcaa taaaacgttc
     1561 gtagtggccc agatccaaat ctgtttcagc accatcatct gttacgaata cttctccgtg
     1621 ctgatatggg ctcatcgtcc ccggatccac gttgatgtat ggatcaaatt tttggatagt
     1681 tacttttaag ccgcggtttt tcaataagcg tcctaaagaa gctgcaacaa tcccttttcc
     1741 gattgatgaa acgacaccgc cggtcacaaa aatatatttt gtcataagtt caaaaactcc
     1801 tttttaattt atgtgcagga aaagtctcag acaataaaca aagctcccta ttcttcaata
     1861 agaataggga gccgaaattt ctgtaaactt ctgaccctct aaaagggtgc ccaaggtgta
     1921 taatacaagc tgactaaggg aaagtcaagt actcaaatga atactatccg cttacaattt
     1981 tttctttttc gatatccttt gagaaattat ttcttaagtc ctttatgagt gcaaaaaaaa
     2041 tttaacccac ataataagct tgatataaac atagctaacg ttaaacctaa gcaaatcaga
     2101 aaatcgttac tgaaaaattt taagtgaaat aatagataaa aaaataaaac aaacattatg
     2161 atagataata tcccacctaa caaagaacta ctttttccta cttgcagcaa cagtttatat
     2221 agcttatcgt agaaacaatc tttttctttc acccttattt acctccttta cgtaaaaaac
     2281 gtagtccaca gttctcggat ttttggcata tcttttctat taaataatat caaaaacaaa
     2341 acaattccac taattttttc aaagatgacc tttttttcaa caaaagtgcc ttgtagggat
     2401 tcattgaagg taacgaatca cataagtaaa aaataaggag atgcgacaaa agtcgtgcct
     2461 ccttatttct aaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     2521 atcaaacatg agaagctgtt tttgaaatta tttcttgtga ttcggagtta aatggtaggc
     2581 tcacaacctc tggataaacg gtattcaaaa gccagtttct cggtattaag ccaaatttat
     2641 gcaaaaatgt agaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacgtctt
     2701 tttcaaaacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagttcagcc
     2761 aattcaaaaa caagaaaagc tgtttttgct tggctattct tattacttgt tgcagggcag
     2821 ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa
     2881 aaaacggcaa aatatgagga actatttttc cgttagtttc tctaatactc aggtcaaaac
     2941 gctgatttca cagcctctaa ttcacggtat tcctgtttta ataaagttct agatattgtt
     3001 ctctttccca ctcggaaaca gtttgtctga atgccgccca ttcgagacgt tttgcttcaa
     3061 caaaatttga gaagatatgt tgtcctaatg cgtcaatcat tacattatcc ttacgcaatt
     3121 ctttgatagc attatggata gttgaaggta agtcctggat atgtgcagct tctctttctt
     3181 cctcatccat tacataaatg ttacggtcaa cagctgctgg cggtgtgatc tgattacgga
     3241 taccgtctag tcctgcttgc aacaatactg ccattgccaa gtatgggttt gcagatggat
     3301 caactgaccg caattccaaa cgtgtggata aaccgcgaga ttctggtaca cgtactaatg
     3361 gcgaacgatt acggccactc catgcgacat aaacaggcgc ttcataacct ggaacgagac
     3421 gtttataaga attgactgtt ggattgcaaa cggctgtgta ggcacgagca tgtttcagca
     3481 aaccacctaa gaaatgataa gctgtttcac ttagttccaa ttcaccgtct ttatcataga
     3541 aaacatttcc ttcatcattg aacaaagaca tattgcaatg cataccagaa ccattgatcc
     3601 caaataacgg tttcggcatg aaagtcgcgt gaagtccatg cttgcgtgca atcgttttga
     3661 caacgagctt gaaggtttgg atattatcac aagcatctac cacatctgca tatttaaagt
     3721 caatttcatg ctgtcctggc gctacttcat gatgtgaagc ttccacttca aagcctagac
     3781 tttctaattc caacacgatg tcacggcggc aattttcacc taaatctgtt ggtgcaaaat
     3841 caaaataacc acctttatca ttcaaggtcg ttgtgatccc accgtcttcg tctaatttaa
     3901 ataagaagaa ttctggttca ggtcctaaat tgaaagaggt aaaaccaagg tcgcgcatat
     3961 ctttcaatgc tcgtttcaaa ttcccgcgag gatcgccggc aaatggtgtc ccatccggat
     4021 tatagatatc acaaattaaa cgagcaactt ttccatgagc actttcccac gggaagatca
     4081 tccaagtgga gagatctgga tacaaataca tatcgctttc ttcaattcga acaaatcctt
     4141 caatcgaaga accgtcaaac atcattttat tttctaatac tttgtcgatc tggctcactg
     4201 gaacttctac atttttgatc gtacctaaga tgtctgtgaa catcaatcgt aaaaatcgta
     4261 cgttttcttc ctcaatgatt cgtttgattt cttcacctgt tatctgtttc tttaccatgg
     4321 tatccctctt ccttttttaa tttattcagt caaagaattg tctttgcaat ccttgcgact
     4381 gataatgacc tgttttgctt aacccacctt gcgccaaaat ctcatcacga aaaatttgtc
     4441 gaacgtcatg atctgtcaac ggcttcttgt tttttgcttc ttgttttttc gcctgtgcca
     4501 tttcgtatac acgtttgatc ccagccatat tcaatccctc tgaaagatag tctttaatct
     4561 caagcaacat atcaatatca ttcagcgaat acatacgtcg gtttccttcg cttcgttccg
     4621 gatgaactaa atcttgttct tcataatagc gaatttgccg agcagacaaa tcagtcagct
     4681 tcataactgt accaattgga aatacggaca tagatcggcg caattccttt tctcccatca
     4741 gacgtcccct ttcgttttat aagttggcta aagtgtacca attaaaaaaa atattgtcaa
     4801 ctgcatatgt taggttttct aacatgagat ctctattttt tataaaataa caaaaaaata
     4861 agaacacaaa aaaggtatct agttgatttt cgtaccagat accttttatt ttttattata
     4921 ctgtactatc ctttattagt gtttttatt
//
LOCUS       DAZUKR010000157         4877 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000157 DAZUKR010000000
VERSION     DAZUKR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.160"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(406..780)
                     /locus_tag="ACG89C_002535"
     CDS             complement(406..780)
                     /locus_tag="ACG89C_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4901031.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="ACG89C_002536"
     CDS             complement(803..2203)
                     /locus_tag="ACG89C_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFS4901032.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="ACG89C_002537"
     CDS             complement(2207..2617)
                     /locus_tag="ACG89C_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4901033.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="ACG89C_002538"
     CDS             complement(2635..3126)
                     /locus_tag="ACG89C_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4901034.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="ACG89C_002539"
     CDS             complement(3136..3927)
                     /locus_tag="ACG89C_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4901035.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="ACG89C_002540"
     CDS             complement(3920..4693)
                     /locus_tag="ACG89C_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4901036.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1550 a          995 c          646 g         1686 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtat
//
LOCUS       DAZUKR010000158         4858 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000158 DAZUKR010000000
VERSION     DAZUKR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.161"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG89C_002541"
     CDS             4..816
                     /locus_tag="ACG89C_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS4901037.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG89C_002542"
     CDS             813..1613
                     /locus_tag="ACG89C_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4901038.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG89C_002543"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG89C_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS4901039.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG89C_002544"
     CDS             3110..4819
                     /locus_tag="ACG89C_002544"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4901040.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
BASE COUNT         1580 a          809 c         1087 g         1382 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag cagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtggtc acagcagccg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcagtaata ggagtttacg agctgtttcg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtattg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattta atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg ttcgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcctgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccggataaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcgt attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct acaccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gattatcgga gacggtggcg caatgggcgg
     3721 gaaagactca aaagaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgcggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtactttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaat ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tcttcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaactg
     4501 gccaaaagga attgcgccat ttgatttgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaaac ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gtgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcag
//
LOCUS       DAZUKR010000159         4836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000159 DAZUKR010000000
VERSION     DAZUKR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.162"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..1809
                     /gene="treC"
                     /locus_tag="ACG89C_002545"
     CDS             181..1809
                     /gene="treC"
                     /locus_tag="ACG89C_002545"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4901041.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(1869..3050)
                     /locus_tag="ACG89C_002546"
     CDS             complement(1869..3050)
                     /locus_tag="ACG89C_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4901042.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            3302..4825
                     /locus_tag="ACG89C_002547"
     CDS             3302..4825
                     /locus_tag="ACG89C_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4901043.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT         1770 a          839 c          966 g         1261 t
ORIGIN      
        1 tttaaacaag aggatagaca aatacatgtc tgatctcttg ttttttattg ggcaacagac
       61 ctcttagaat gtataaagat aaattttaca aacgctttct gttcagttat aataataaat
      121 gataacttgt atatataagc tgtggtaggt aataaatcag ttgaatggag gtaaatatcc
      181 atgacattta aagacaaagt catttaccaa atctatccca aatcttacaa agacagtaat
      241 ggtgatggaa ttggagattt aaaaggaatc aaagaaaaac tcccgtatct gaatgatcta
      301 ggcatcgaca tgatttggct aaaccctatt tatccaagcc ctcaaaaaga caacggttat
      361 gatatctctg attacacagc catcgatcct atctttggta cgatggagga attcgaacag
      421 ctaatagccg aagcaaagaa ataccggata gaaatcatgc tcgatatggt gctgaatcac
      481 gtttcgacag aacatgaatg gttcaaaaaa gcactagcag gggaccagca ttatcaggac
      541 ttcttcattt taagagaaga gccaacggat tgggtatcaa aatttggtgg aagtgcttgg
      601 tcgccatttg gagatacggg gaaatattat ttgcacttat atgatcggac acaagctgac
      661 ttgaactggc ggaaccaagc agtaagagag gaactattca aagttgttcg tttttggatg
      721 gaaaaagggg taaaaggatt ccgtttcgat gttatcaatg ttatcggtaa agacgaacaa
      781 ttgaaaaata ataaagaaaa tgatggcaaa cctgaatata cggacaaacc aatcacccat
      841 acgtatctaa aagaattgaa ccaagcaacg tttgggacgg attccactat catcacagtc
      901 ggtgaaatga gctctacgac gatcgaaaat tgtatcttgt atacagaacc tagtagagaa
      961 gaattatcaa tggtctttaa tttccatcat ttgaaagtcg attatgaaaa tgggaataaa
     1021 tggtctactc ctccatttga ttttgcagaa ttaaagcgac tatttcatac atggggagaa
     1081 aatatgagtg agggtaacgg ctggaatgct ctatttttta ataatcatga tcagccacgt
     1141 gcgctcaatc gatttgtagt tatcgatcag tatcgaaaac aaggagcaga gatgctagca
     1201 accatgatcc atttgaaccg tgggacgccc tatatttata tgggagaaga aatcggcatg
     1261 atcgacccag actattcatc gatttccgac tatattgacg tagaaagttt gaatgcttat
     1321 aagttactcc ttgaacaagg attcacagaa aaagaagcgt ttcgtcggat caaagcaaaa
     1381 tctcgcgata actctcgaac acccatgcaa tggtcagaca cagaacaagc aggatttact
     1441 acagggaaac cttggttaaa agttggaggc aagctagaag agattaatgt agaaaaagaa
     1501 ctatcttctg aagattctat tttgtcctat tacaagaaat tgattcggct gagaaaaaca
     1561 tatccaatcg ttgcacaagg agattatcat gcttatgaag caaatcatcc ggaagtttat
     1621 ggctttcttc gccaatttga aggacagcag ctgcttgtat taacaaattt ctatgataaa
     1681 gatacaagga tcgccatccc agaaggattc acagaagcca atattcttat cgataattat
     1741 tccgatactg ttcttcatca aagtctggag ctaaaaccat accaggcaat tgctttgctg
     1801 aaaaattaaa acaaaaacac aaaacgtcgt tcctacgagc tattctcgta agatcgacgt
     1861 tttgtgtttt atttaaaata atcttcttca gaaaatgaag agttttcata ctctccttta
     1921 tcgccataaa atttattgta gcgttgtttg aatttacgga aaataaaagt aacgagtaat
     1981 ttgactaacg tatagatagg aataccaaaa ataacgccca caattcccat tagatcaccc
     2041 atgacaagta aaacgattaa gatcgtaatc ggatggatct gtaaacgatc acccattact
     2101 cgaggaatca ccaagtcacc gtggagaagt tgtacggcaa accagacaat gacaaatttg
     2161 acagccataa aagtagagtc ttggaatgca atgatcaaac ctggaaagaa ggcgataaac
     2221 ggcccaatat atggaatgat acataacaac ccagcagaaa tagctaaaac actagcaaat
     2281 tctaatccga tcagtaagaa acaaacccag tagactgctc caaggataaa tgaagcaatg
     2341 atttgcccct ttagaaatga acccaattgt tgattagcaa tctttgtcaa atttttaaaa
     2401 tctgtacgaa atgctggtgg gacaatcgct aaaactgact gataaaattt ttgaggatct
     2461 ctcaaaagga aaaaagcgac aattggacca gtaaataaag taataaaaat agctgtaaca
     2521 gttgaaaaaa tgttgctgat ccgttgtgcg ccactttccc aataatttcc aataaatcca
     2581 gctatatcag acgtcacgtc gtcaatagaa gcaaaaaatt gattaaatga atcggcaaaa
     2641 ggtgttttct ccaaaaattg tcggagactc tcttgaaaat cttgaaacaa atctggcaaa
     2701 ctactgatca atgtctttgc ctgttcttca agaaaaggaa taagccatat gcttccaagc
     2761 cccaataaaa ataaaataat cacataaacg ataatgactg acaagttcct cgaaaggtgc
     2821 ttgctcaaac gtttgatgac tggttccaaa agataataca agatcagtgc aaacaataat
     2881 gggggcaaga tagtagcgat aatgattccg acaggcttga atataaacga gatctgatgg
     2941 aaaaaataaa tgatcagcgc aagtaagata actattccta aaacatagta agaggttttt
     3001 cctcctaaaa agcggatcaa acgattcgtt gtatctgttt cttttttcac tctttttcct
     3061 cctttatcta taacttcatt atagacaaaa gcgggtataa aaggaaaatg atagaggttg
     3121 attgcaaaaa tatcgtcaat cattcatata aactttctca tcaattaaaa taagaaaacg
     3181 cttgacagtt cttttaacca tatgataaat taaatcctgt tgctaaaacg taactattac
     3241 ggtttgcgaa aagcgaatcg gaacattcaa atgaaagaag atgagaagag gagtgtcata
     3301 aatgaaacga ttgataggta tattggccat gctggtttta gcaggtatgt taactgcttg
     3361 cggggcaagt gggaaagccg aggacagtaa agaaaaattg agcgtgatga cgacctttta
     3421 tcccatgtat gattttacaa aagcaatcgt cggggatgaa ggagaagtag agttattgat
     3481 tccagcaggg actgattccc acgattatga accttctgct aaagacatgg caaagatcca
     3541 agatacagat atctttgttt ataatgatga aaacatggaa acatgggtcc ccgcaatcca
     3601 aaaaacatta caagaaggca atgtccatac gataaaagcg acagaaggta tgcttttact
     3661 tccgggaagt gaagaaggac atgatcatga ccatgaacac ggggaagaag gccatacaca
     3721 tgagctagac ccacatgtat ggctagctcc aagtttagca atcaagcagg tagcaaacat
     3781 acgtgatcag ctgattgaag cttacccaga aaaacaggaa gtatggacaa aaaatgcagc
     3841 agcctatacc gagaaacttc aagcactcca tcagctttat caagaaacct ttaagcaagc
     3901 aaaacaaaga tcttttgtca cgcaacatgc tgcattcaac tatctagcgt tagaatatgg
     3961 attgaatcaa gtatcgattg ctggtctttc ttctagtgaa gaaccgtctg cggcgagaat
     4021 tgctgaactg aaacactttg ttaaagaaca tggaatcaac tatatttatt ttgaagaaaa
     4081 cgccaaagat agtattgcca gaacgttagc aaacgaagca ggtgtatctt tagaagtatt
     4141 gaatcctttg gaaggattga cgaatgaaca aatagaaaat ggagaaaact atctttctat
     4201 catggaagca aacctcgaag cgttgaaaaa aacgacagag acagaaaacc cgctagaaga
     4261 tactttgacc cccaaaaaag aaaagagcgt ctacaacggt tattttgaag acgaggatgt
     4321 acaagatcgc tctctttcag attgggcagg aacttggcaa acagtagata cgtatgtaga
     4381 agatgggact tttgatcctg tttttgaata taaagaaaaa ttgaatcagg ataagacagc
     4441 acaagaatat aaagaatatt atacgaaggg ctatcaaaca gatattgctt caatcaagat
     4501 cagtgagaaa gagatggcat ttacgtttaa ggatggaaca acgaaacgtt caaattatcg
     4561 ttatgtaggg aaagaaatct tgacctacga ggcaggaaac cgtggcgtaa ggtatctatt
     4621 cgaagcagaa gataaggaaa gtggcgcttt tagatatatt caattcagtg atcacacgat
     4681 tgcaccggct gactccgaac attttcattt atatgcagga aatgaaagtc aagaagtatt
     4741 gctgaaagaa atggagaatt ggccaacatt ttatccagat acaatggatg gacgtgaaat
     4801 tgcacaggaa atgcttttgc actgataggc aaaaaa
//
LOCUS       DAZUKR010000160         4810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000160 DAZUKR010000000
VERSION     DAZUKR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.163"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1118
                     /locus_tag="ACG89C_002548"
     CDS             72..1118
                     /locus_tag="ACG89C_002548"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4901044.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="ACG89C_002549"
     CDS             1135..2358
                     /locus_tag="ACG89C_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4901045.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="ACG89C_002550"
     CDS             2447..3334
                     /locus_tag="ACG89C_002550"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4901046.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="ACG89C_002551"
     CDS             3420..3650
                     /locus_tag="ACG89C_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS4901047.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="ACG89C_002552"
     CDS             complement(3707..4672)
                     /locus_tag="ACG89C_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4901048.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1629 a          795 c          975 g         1411 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctatag ttctgttcac agaaaatact ttgataatca aaatatgtga gatagatgat
     4801 ttttctaata
//
LOCUS       DAZUKR010000161         4788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000161 DAZUKR010000000
VERSION     DAZUKR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.164"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACG89C_002553"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACG89C_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS4901049.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACG89C_002554"
     CDS             1019..1183
                     /locus_tag="ACG89C_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901050.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACG89C_002555"
     CDS             2141..3118
                     /locus_tag="ACG89C_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4901051.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACG89C_002556"
     CDS             3128..3625
                     /locus_tag="ACG89C_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4901052.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACG89C_002557"
     CDS             4001..4282
                     /locus_tag="ACG89C_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901053.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACG89C_002558"
     CDS             4284..4700
                     /locus_tag="ACG89C_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901054.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAZUKR010000162         4714 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000162 DAZUKR010000000
VERSION     DAZUKR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.165"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..359
                     /locus_tag="ACG89C_002559"
                     /pseudo
     CDS             108..359
                     /locus_tag="ACG89C_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="ACG89C_002560"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="ACG89C_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4901055.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1739
                     /locus_tag="ACG89C_002561"
                     /pseudo
     CDS             1656..1739
                     /locus_tag="ACG89C_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1788..1871
                     /locus_tag="ACG89C_002562"
     CDS             1788..1871
                     /locus_tag="ACG89C_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS4901056.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG89C_002563"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG89C_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4901057.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="ACG89C_002564"
     CDS             complement(2996..3859)
                     /locus_tag="ACG89C_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4901058.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="ACG89C_002565"
     CDS             complement(3861..4133)
                     /locus_tag="ACG89C_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4901059.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="ACG89C_002566"
     CDS             complement(4150..4365)
                     /locus_tag="ACG89C_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4901060.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4714)
                     /locus_tag="ACG89C_002567"
     CDS             complement(4457..>4714)
                     /locus_tag="ACG89C_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFS4901061.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1606 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tcat
//
LOCUS       DAZUKR010000163         4634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000163 DAZUKR010000000
VERSION     DAZUKR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.166"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACG89C_002568"
     CDS             174..518
                     /locus_tag="ACG89C_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4901062.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACG89C_002569"
     CDS             519..938
                     /locus_tag="ACG89C_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4901063.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACG89C_002570"
     CDS             complement(1030..1380)
                     /locus_tag="ACG89C_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901064.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2676)
                     /locus_tag="ACG89C_002571"
                     /pseudo
     CDS             complement(1346..2676)
                     /locus_tag="ACG89C_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            2932..3498
                     /locus_tag="ACG89C_002572"
     CDS             2932..3498
                     /locus_tag="ACG89C_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4901065.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="ACG89C_002573"
     tRNA            3643..3730
                     /locus_tag="ACG89C_002573"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="ACG89C_002574"
     CDS             4064..>4634
                     /locus_tag="ACG89C_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4901066.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DAZUKR010000164         4611 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000164 DAZUKR010000000
VERSION     DAZUKR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.167"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..400)
                     /locus_tag="ACG89C_002575"
     CDS             complement(86..400)
                     /locus_tag="ACG89C_002575"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4901067.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(451..705)
                     /locus_tag="ACG89C_002576"
     CDS             complement(451..705)
                     /locus_tag="ACG89C_002576"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4901068.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACG89C_002577"
     CDS             complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACG89C_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS4901069.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="ACG89C_002578"
     CDS             complement(1145..2098)
                     /locus_tag="ACG89C_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4901070.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="ACG89C_002579"
     CDS             2710..4203
                     /locus_tag="ACG89C_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS4901071.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="ACG89C_002580"
     CDS             4338..>4611
                     /locus_tag="ACG89C_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4901072.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DAZUKR010000165         4579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000165 DAZUKR010000000
VERSION     DAZUKR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.168"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACG89C_002581"
     CDS             complement(<1..205)
                     /locus_tag="ACG89C_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4901073.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLN"
     gene            complement(536..769)
                     /locus_tag="ACG89C_002582"
     CDS             complement(536..769)
                     /locus_tag="ACG89C_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4901074.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(784..1734)
                     /locus_tag="ACG89C_002583"
     CDS             complement(784..1734)
                     /locus_tag="ACG89C_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS4901075.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1731..2573)
                     /locus_tag="ACG89C_002584"
     CDS             complement(1731..2573)
                     /locus_tag="ACG89C_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS4901076.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2575..3294)
                     /locus_tag="ACG89C_002585"
     CDS             complement(2575..3294)
                     /locus_tag="ACG89C_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4901077.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            3842..4144
                     /locus_tag="ACG89C_002586"
     CDS             3842..4144
                     /locus_tag="ACG89C_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901078.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            4216..>4579
                     /locus_tag="ACG89C_002587"
     CDS             4216..>4579
                     /locus_tag="ACG89C_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4901079.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAM"
BASE COUNT         1575 a          802 c          606 g         1596 t
ORIGIN      
        1 agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca ttaatatgcg
       61 gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa aaagcacccg
      121 tagttacgcc tgcggtcagg cacagttgcc ttatcttagt ttgctgaaat ccttcttttt
      181 caaaatggct tagtccactt tctattaact tttggtgagt tataatataa tctctggttt
      241 ctatgggtac ttcctcctaa taagatgaat taactttatt atcttacaca ggacttgaag
      301 tttcaatcgt taacgtttat tactgaactc attccttaga gctaaactaa tgagaaagag
      361 cagaataact gatacaaaaa gcaatcctac aagaattaat agactcatct aaatcattca
      421 ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat taggataagt
      481 cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa gtcttttact
      541 taaaaccttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat tgaaatccta
      601 tatctaaata gtcctcatat ctaagtttag ccagcatctc acttacagat gctggactaa
      661 cacttagaac ttttgctaaa tcattatttt ttaatttctt ttttcttttt gataattcct
      721 gaattgctag taaataaatt agtatagtag tcgttttaat ttgtaccata cctaccctcc
      781 aaattatttc gaaaggcctt cagcaatttt atccaggttc catttcatca tcgcatagta
      841 actatcacca tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg aatagatagg
      901 aatattggta tcttttgata ctgttttcat tggtctatca tccacactgc tttctacgaa
      961 taaggaggga acttttgttg tgcgtaattt ttcaactaag tgttttattt gatctggtgt
     1021 tccttcttct tcagtgttga tttcccaaat gtaagcagaa ggcacattat acgctttcga
     1081 gaaatattta aagcatcctt cacttgtaac aatcattttt ttatcattcg gaattgaagc
     1141 aaatttagat ttagcttctt tgtctagaga atccaatttt tcaatatact tatctagatt
     1201 ttctttatag aattttttat tatcaggatc tttttccgct aactgttttt caatattttt
     1261 agcgtaaata ataccgtttt ctaaatttaa ccaagcatga ggatcttcct tccctttctc
     1321 actctgaccc tctaagtaaa taacatctat gccatcactt gctgcaaaat agtctttgtt
     1381 ttcctctttg ttcgcatttt taactaattt tgtaaaccaa gcatttcctc cagtttccaa
     1441 gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat cttcaggcaa
     1501 aggttcatat tcgtggggat ctttcccaat aggtacgata ctgtgtaaat caattttatc
     1561 ttttgctata ttttcggtaa tatctgctaa aatcgaatta gtaactacta cttttaattt
     1621 ttcgttcgaa gccactttat ctttgtcttt actatttatt gaacatgccc cgaatataaa
     1681 aagagcaatc aaaatcccca gacttcccag tactgctttc cattttctca tcataacgat
     1741 ctctccttct ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat tacaaataag
     1801 aatgccgctg ttaaaacaat acttgatcct gctgctatgt taaaactata accaataaac
     1861 aatccaagca atgatgaact tgctccgact gttgcagaaa taaatatcat agttttcaag
     1921 ctatttgcat ataaatatgc tgtagctgct ggtgtaatta acattgcaac aattaatata
     1981 gttcccacac tttgcatagc tgttactgct actaaagtta atagaaccat caatagataa
     2041 tgataaaaac tgacattcat ccctattgct tttgccatta atggatcaaa agaagtgatt
     2101 aataattctt taaaaaatag aactactata accaacacta aaaccgaaac gccaattgta
     2161 atccacttat caatatcttg tacagctaaa atatttccaa ataaaatatg aaataaatcc
     2221 gttgagctat ttgcaactcc tataaggata actcctagtg ctaaaaaaga actgaaagta
     2281 ataccaattg ctgtatctcc ttttattaca ctattgcttt ttataaaagt aataatgata
     2341 gaagctaata gaccaaatac aatcgcacca ataaaaaaat taatacctaa aatataagaa
     2401 attgctactc ctggcaaaac agcatgagaa atagcatcac ccataagaga cattcctctt
     2461 aaaataataa aacaacctac tgttccagaa acaatcccta taactacaga tgtaattaga
     2521 gcattctgta aaaagtgaaa gtcatacaga ccattaataa aattttgaag catattattc
     2581 cccctctcca ataaatatag tatctccata ggcttttttt aaattttctt cattaaatac
     2641 agattctaca cttccgtgag caattagatt tcgattcaaa agaataatat tatcaaaata
     2701 cttctttacc ttgcttaaat catgatgcac aattagcact gtttttccct gatgtttcaa
     2761 tttttttaac gtatccataa ttattcgttc actaactaag tctatcccta caaatggttc
     2821 atctaaaaat atgaaatccg cattttgagc aagacatctt gctaaaagta cacgttgaaa
     2881 ttgtcctcca gataattctc ctatttgatg attagaatat tttcccatat cgaccttttc
     2941 caaagccttt gaaactcttt gccattctgc atttttaatt ctttgaaaaa ctttcattcc
     3001 ggcatatgtt cccatagata cacattcttt tacttttatt ggaaaagtaa aatcaatatc
     3061 agctttttgc tctacatatg caatcttcac cagtttttta ttgatatctt cattattaat
     3121 ggagacctct ccctgatgag ggataatatt taacattgct ttaagcaatg tggattttcc
     3181 agcaccattt ggaccaataa tacctgttaa agcaccttct tgtattttta aagaaatctc
     3241 gttcaatgct aaaaaatcgt tataacaaac attaagcttt ttcacttcta acataacttt
     3301 ctccttctaa atgataatga ttatcacgat catatggtat acaaaaatta tattttagtc
     3361 aaccctaaat tttatagttt agattttttt ctgttgctga agattagaat gacaaaacta
     3421 tttgaaacta cgcgtatagt aagtagcttt caaaatatat aaaaaaggag cgttctcaaa
     3481 tgagaacatg ctcctaaaac tactttattg gcgtatggaa aaattatact tgaagtggat
     3541 agcgggttga ataagttgat aagcatcaaa acatttataa aaattattca agactatgtc
     3601 tgttccatat acaaatatga aaatcagtaa actccattta acttttttcg tagtgtgtct
     3661 ttttatacat gtttatcgtt attaatagtg aataggaaaa aaactatttt tgatcattga
     3721 caattaaata caatttttga ataacgatcc ctgctattcg agctagaaac tcaagtacgc
     3781 catgacctga ttagcgacaa atacttagag atttcttttt gttctgggaa aacaaaatgc
     3841 catgacaata gcagcaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt
     3901 gaaattccta tgtgcttacc caaagcaatt caaaaatttc ataactacta ttttaggaga
     3961 tgattttata gaatccagag tctacctgaa taaaaaacaa acctgtgcat ttctaggaat
     4021 cgataataat actctagata agtggattcg tatatataat ttcccagtaa taagagttgg
     4081 tggtgtagtt cgctttgacg tagaagatat taaagacttt atgaatacat ttaaaataac
     4141 ttgaaatttt cttctcgccg catatactaa aattagtgaa atatactgcc ttactttttt
     4201 taaggagtga ggattttgcc tacaaagctt tcaaatggga aatataaaac taatctgaga
     4261 tatccaaaaa ggtttaaaga aataacagga attgcatcag aaaagtatca aaaaacattt
     4321 ccaaataggc aattggcgat taaagccgaa aatgatatga agaagaaaat agaaaaagtt
     4381 ttgagagagg agaatgcaaa ctcattagaa ttaaaaggaa aaataacgtt taaaaaattt
     4441 tacgaatcta aatggcttcc tagatatgaa ttaggtcaaa caattagatc aaacaggcct
     4501 cctagtgata taaccattag taatacaaaa gatatatttc ggttgcatat tttacctatg
     4561 tttggtgaat acgcaatga
//
LOCUS       DAZUKR010000166         4508 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000166 DAZUKR010000000
VERSION     DAZUKR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.169"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            612..1523
                     /locus_tag="ACG89C_002588"
     CDS             612..1523
                     /locus_tag="ACG89C_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFS4901080.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2010..2276
                     /locus_tag="ACG89C_002589"
     CDS             2010..2276
                     /locus_tag="ACG89C_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901081.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2348..2689
                     /locus_tag="ACG89C_002590"
     CDS             2348..2689
                     /locus_tag="ACG89C_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901082.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2744..3163
                     /gene="ssb"
                     /locus_tag="ACG89C_002591"
     CDS             2744..3163
                     /gene="ssb"
                     /locus_tag="ACG89C_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4901083.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3240..3536
                     /locus_tag="ACG89C_002592"
     CDS             3240..3536
                     /locus_tag="ACG89C_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901084.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3551..3982
                     /locus_tag="ACG89C_002593"
     CDS             3551..3982
                     /locus_tag="ACG89C_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4901085.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4287..4469
                     /locus_tag="ACG89C_002594"
     CDS             4287..4469
                     /locus_tag="ACG89C_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901086.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1651 a          714 c          962 g         1181 t
ORIGIN      
        1 gtatgaaagg cattctccga cttccaggtt tttgaccatt ttctctcaac gtggaaaaac
       61 cgccacttat gagagaaaat ggtcaaaaac ctaagcggga agtctccgaa taacatacca
      121 agcgccgaca gccaacagat aaagaataca ctactaaaaa tagtagtagc ataaaattta
      181 aaagggaaaa aatcaacccc taccccagtc ttggggtctc aaaactaact cccccctaac
      241 cccctctttc agcctctttt gaaagagggg gagaatcaac cccacggcca acgccccagt
      301 gttcttgggg ctgtccagaa cagaaacttg cgagagagtg ggggtaaaac aagtgtagag
      361 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ccggagtaaa acaagtgtag
      421 aggaaacttg aagacttgtt cgaacagaaa cttgcgagag agttggagta aaacaagtgt
      481 agaggaaact tgaagacttg ttcgaacaca aacttacaag agaggggagt aaagcagttg
      541 cagaggaatc aggaagattt gttcgaacag aaacttgcaa gaaagcatta ttaaattgaa
      601 ggtgatgaaa tatgaagtat ttattgcatg gctattttat tgattattct actcgcaata
      661 gtagtacttt agcgattcta tgtttgcttc atgaattgag agtggttact agaaaacagc
      721 tatacaaact attgaatttt gacagatcaa ttagtaaaca aggagtaacg aatgctttag
      781 tgagattata tgaaaaacag ttaatcgatc gaaaaaaata tggaacagaa atgtattatt
      841 atttgacgaa agagggacat caaagcatag gtgggtatta tacgttgccg aaagttcctg
      901 aatataattt gaatcaccat ttacaaatta acaattattt gattaaagga atggcattgg
      961 caaaagacca cccacattta aaacggatta tttcagaaag acgtaccgtc tatgaaacga
     1021 aggatttaaa tcctaagaca aaaggaagaa aattttttgt gccagatttt atttttcgtt
     1081 ttcgagacaa agaaaatcat gagatcaatt ggtcctttga aattgagcta acgttgaaaa
     1141 cacgtcgtcg ctaccgtgaa gcgatttttc cgaaatatat tcgtgagttg cgaacacgtc
     1201 ctgaatcaca tttaatttat gtgacaccta gtggcgcaat tcaagaagaa cttcatgcgt
     1261 ttaagcgtat ttttttcaag cgcgaaactg aaaaagatgc gagtcaacaa gaattgtttg
     1321 agcggttgca tattttttca gctttagagt ttgaagaggg attaaaagca ttattagaga
     1381 atgatccagc agtcaattgg cggacagtag aacgagaaaa aatttatccg aatggcgctg
     1441 ttcaaagtcg tttgaattta tttgaagaat caactgaaaa tgaggaaaaa ccaaaagtga
     1501 agtttacgcc tgaagtattg taaaagaggg tgttcgcttc gctctttttt tgtgccagtt
     1561 tgctggcacc gttcagacgc aagcgtctga accggctcgt ttttaggaag gactaacttg
     1621 tttgatttat ccgttgtcaa agaagtggac gttcgagagt ccctaatcgt atagcagtca
     1681 ttcggcgagt ataacctaaa acataacctg ccaattgcca agggtaaaat gcacgctgcg
     1741 cgcccttggc aatcgtcaga acatgttttc gagccggtta tgccgaacga ccgctatacg
     1801 aaaagggcct ctaaacagaa gtccactttg atccttgaca actgaaaccc aaaatcaaac
     1861 aagcaggcct tcgtctaaaa acgagccgaa tgaagacctt gggggttgca cccccaaacc
     1921 cccgtgcccc cgacacacta ccgaaaaggt gggggtgggt ggcgaaaaaa tcaaataaaa
     1981 cattctattc aagaaaggaa gaaacaacca tgacaactaa aaacgagcaa ctgaaagaac
     2041 aggcaagaaa agagttgcaa gaaagaggtt tgattattga ggggagcttt gaaggagatt
     2101 ttgaaaccta tattggttgt tatgcaagac cgataaacaa acctacagcc ttagacccaa
     2161 tgaacgaaca agaagtaaaa gaacaagaaa aacatgcagt gaatggtttt ccgcaagatt
     2221 tcactgaatg gtatgagtgg gaaattaaca acgggaagct tgaaaacttc ttataaaagt
     2281 cagatgacgt gcaaagagaa tgattctctt tgtacggtaa aaatataaag aaagaaggaa
     2341 aaagaaaatg gcaaaaaaca caccttatca agtaggagac attttttata gtagttgggg
     2401 ctacgaacaa acgaacgtga ctttttggca aattgtgaag ttaacagaaa aaacagcatg
     2461 gtttcagcct attcaaaaga aaacggtcga gcattattcg tccatgagtg gaaaagttgt
     2521 tccaatgaaa gacaagttta tagagtatcc tttagcaaaa actaaagaca gtattgtaaa
     2581 aaaacgaatt gatccagaaa cctatcctga tatgttatgt ggttatcgta gttgggacac
     2641 gtttcataga tataaaaatc aagctgttta tgaaagtagt tattattaag gggatagttt
     2701 cccctttcaa aaataataaa tagaagaatg gagagaaaaa atcatgatga attcagtgaa
     2761 tttagtagga cggttaacaa aagaagtgga tttaaaatac acaccaaaag gcaatgcgac
     2821 aggcacattt attttagctg ttaatcgaaa ttatacgaat gcaaacggcg agcgtgaagc
     2881 agattatatc cgctgtgtca tttggcggaa agctgcggaa actttagcaa aatatactgg
     2941 aaaaggaagt ttgattggca ttaatggacg tcttcaaacg aggagctatc agaatcagca
     3001 aggacagacc atgtatgtta cggaagttct agtgacagat ttctatttgt tagagagtaa
     3061 agaaatcaat gagcaacggt caaaatcaat ggcgaataat caaaataatt cttcaattcc
     3121 aggaaatgag attcaaattt cagatagtta tttgcccttt taagtgaaaa ataattttgt
     3181 tctatacctg atttcttcag gtatagaaca aaaaataaat agagatgagg cgatttttta
     3241 tgaaaatcat tgacagttac gatcaacgaa ccatgcaata tttgggctcg tttgattata
     3301 cagatgaaaa tattattaat tatgttgcaa gtttacctcc ttatcaaagc gttcgcttgg
     3361 tagattttgc aagtgatgaa ttggtattaa caacgatggg caacttttta gattatgtcg
     3421 gagacaaaaa atggttggaa aaaattttgt ctaaattgat tgcgaaacaa acaggagaag
     3481 aacccataaa tgcgattcaa ttgttcaatc gatatgaaca agaaaacaat gattaaagag
     3541 gagagaaaaa atgaaaagat taaaacgaag tatttttatt gtgctagcat tgattgattt
     3601 tgttgggaca ggttgtgaag caattgacgt tcaaaaagct cccttatcgg aaataaaaca
     3661 agcaataaca aaacaagatg aagaacaacc gctaaataca caagtagaaa tagtgaatca
     3721 aaataaaccg agtttttctg aagaagattt ttcgttagaa aatggaacca ggcaattttt
     3781 ttcagatttg gatcgtttaa atcgggtagg tgtagcaagt gcacttttgc ataaaagcag
     3841 gatgccaaca gaagaacgag accgattata cattaagcca actggctgga aacaaaagaa
     3901 aatgaagaat ggcgattatc tttataatcg ttgtcatttg attggctatc aacgtgcgac
     3961 aagtttcccc ataagtatgt aatacgaaac gggaggatta atcttaagat taatcacaac
     4021 taacctattt gacagttgga atgaaagcta tcatgtctgc tgaatcaact ggtctgatac
     4081 tcctacatgc aataaagtct actaggaagt tattgtatga agctaggtaa aggcggcaga
     4141 acaatttcta agtgtggaat catatgaagt tggatatgcc gggacgctat aaattactca
     4201 gggtgagact gtttttaatt aaactgacga acttgcgaat gtacgggtct ataaccgact
     4261 tatattcgaa agatataaga ttgcgaatgc aaagaacatt atcgaaaagt aagaatgttg
     4321 gtcatgaaat tcgagatgga agactagcgg tgtatctgtc aagagttggt aaaacatgtt
     4381 tgattgattc agatgagagt gggaatgcga caaaacgttt tacagaagaa atagagcaac
     4441 tagcagaact gaatatacga tatttttaaa tactttagcg gagaataaaa aaatcctcta
     4501 cagaaaac
//
LOCUS       DAZUKR010000167         4491 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000167 DAZUKR010000000
VERSION     DAZUKR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.170"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..719
                     /locus_tag="ACG89C_002595"
     CDS             <1..719
                     /locus_tag="ACG89C_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFS4901087.1"
                     /translation="LSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPV
                     LLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGY
                     SDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIF
                     LGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYI
                     GKKVKRPISADSVLLEEDVE"
     gene            716..1864
                     /gene="neuC"
                     /locus_tag="ACG89C_002596"
     CDS             716..1864
                     /gene="neuC"
                     /locus_tag="ACG89C_002596"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4901088.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1837..2514
                     /locus_tag="ACG89C_002597"
     CDS             1837..2514
                     /locus_tag="ACG89C_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4901089.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2465..3646
                     /locus_tag="ACG89C_002598"
     CDS             2465..3646
                     /locus_tag="ACG89C_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4901090.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            3659..>4491
                     /locus_tag="ACG89C_002599"
     CDS             3659..>4491
                     /locus_tag="ACG89C_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFS4901091.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     N"
BASE COUNT         1629 a          579 c          885 g         1398 t
ORIGIN      
        1 ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag
       61 gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt
      121 atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg
      181 tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta
      241 gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg
      301 ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg
      361 ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag
      421 cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat
      481 ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa
      541 gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc
      601 ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca
      661 aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa
      721 aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga
      781 gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca
      841 taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt
      901 tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat
      961 aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga
     1021 tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca
     1081 tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa
     1141 aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg
     1201 ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa
     1261 tagattacct agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa
     1321 atacgttttg caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat
     1381 tttagctttg atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa
     1441 tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat
     1501 tatttatatt acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt
     1561 tatgatcgga aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat
     1621 aaatttagga accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct
     1681 aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag
     1741 cttgaaaaat gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac
     1801 attgaatcaa atattgaatg atggggaaga tattaagtgg attcaaaaac aaatagctta
     1861 ttaaaatata ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt
     1921 attgaaacct taagattaga aaataagtta atatatggat tttttgatga taagcaaaat
     1981 aaaatgcaag atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc
     2041 aaaaaaaaaa tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt
     2101 ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata
     2161 gtggcaaaag aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac
     2221 ccagatgctg taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat
     2281 tgtagaatta ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat
     2341 gtaggtgtag gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga
     2401 tcttggtgta tgataggcgc agggagcact attgttaagg atattccctc acattcctta
     2461 gcttatggca atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt
     2521 attatcaagt cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga
     2581 taattggata gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata
     2641 tactggttct aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc
     2701 cttaagactg cttaatgttg gaaaaaacga tattgtcttt gctccttcat ttacttttat
     2761 agctacagta aatcctattt tatatcaagg ggctgtacca gtgttcattg actcagaacc
     2821 taatacatgg aatatgagcc ctgttgcact agagagcgca ttagaagaag ctgaaagaaa
     2881 caaccaatta cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg ctgattatga
     2941 acgaatttta gctattgcaa accaatttaa tgttccagtg atagaagatg cagcggaatc
     3001 gttaggttct acttataagg gaatttcgtc aggcttattt gggacaattg ggatctattc
     3061 atttaacggt aataagatca tcacaacctc aggtggagga gctctagtgt ctagtgataa
     3121 aaaatatgtt gagcattcaa gatttttagc aacacaagca agagatcaag caccgtatta
     3181 tcaacatagc gaaataggtt ataattaccg tctaagcaat atttctgccg gtatcggacg
     3241 tggacaaatg gaagtattga atgatagagt agctaaacga agagaaattt tcaatcgata
     3301 taaggaagct ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg atggcatggt
     3361 gccaaatcgt tggttgtcta ctttaacgat caatgattat aattctattg atcaagtagt
     3421 taacgtaata aataaactaa atgaaaaaaa cattgaaaca agagctttgt ggaaaccact
     3481 acatttgcaa ccagtatttc aaggggtaaa aatgtattac catgataata atcttagtgt
     3541 gtcagaaaag ttatttaata gcggcttatg cttaccttct ggaagtaata tgacagatag
     3601 tgaacaagaa tttgttattg atgagtttaa aaaaatattc aagtaggtgt gatattttat
     3661 gaacatagga attattggtt taggttccat ggggaaaaga agacttagac tcattcaaga
     3721 acactttaat gatattttac tatgtggtgt tgatactaat gaggcgagac gaaaggaagt
     3781 tgaaaaggaa tttggaatac caacttataa tagtctaaca gaggcaaaaa atttgtttga
     3841 aataaattca gtttttgttt gtacatcccc tataacccat gaatttatta taaaagaggc
     3901 tttaaattta gattgtaata tctttacgga aatcaattta ttaaatgaat attatgatga
     3961 aataatcgaa gtagctaaaa agaaagatag attactttat ctatcttcta catttttgaa
     4021 gagaaaagaa attcaatata ttaagtctaa agtgacgcat aataaaaaaa tttcttatca
     4081 ataccatgta ggacagtatt tacccgactg gcatccttgg gaagattatc gcaatttttt
     4141 tgtttcaaac aaaaaaacta atgggtgtag agaaatattc gctattgaat taccttggat
     4201 tatagataca tttgggaaag ttaaatcgtt tactgtaatg acccagaaat tatccaatct
     4261 tgatattgat tattcggaca gtttcactct tattattgag catgaaactg gtatagttgg
     4321 aactttaagt gtagatgttg tttcaagagt agctaaaaga aatttaacta taattggtga
     4381 aaagatttta atagaatggg aaggtaaccc ggattctcta ttttcatgga atgaagaaac
     4441 ttctagtatg acagatattc aattgtacga tgaggttatg catgataata a
//
LOCUS       DAZUKR010000168         4463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000168 DAZUKR010000000
VERSION     DAZUKR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.171"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            314..1042
                     /locus_tag="ACG89C_002600"
     CDS             314..1042
                     /locus_tag="ACG89C_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4901092.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1187..3214
                     /locus_tag="ACG89C_002601"
     CDS             1187..3214
                     /locus_tag="ACG89C_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS4901093.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3322..4419
                     /locus_tag="ACG89C_002602"
     CDS             3322..4419
                     /locus_tag="ACG89C_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4901094.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1565 a          717 c          923 g         1258 t
ORIGIN      
        1 atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc aagtcacatt
       61 ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct gtatttctag
      121 attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga taatcattta
      181 taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca taagtatcgt
      241 aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa ataaagaaag
      301 aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt tattcagcat
      361 ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat ataacacaga
      421 aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg atccagagaa
      481 tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa caggaggggc
      541 attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt ctactgcgga
      601 taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac cgacatatgt
      661 ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct ctcaaccaga
      721 acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat ttacgaaagg
      781 gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt ctgaattaac
      841 gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa caggtgtggg
      901 gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg tacagctttc
      961 ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag tttggacatt
     1021 agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa tacgtagtca
     1081 tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat ttgtttggat
     1141 acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga aaaagaggag
     1201 gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca tagggtactc
     1261 tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca aactcacttc
     1321 gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg atttaagtat
     1381 ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca aacaaggact
     1441 agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta tttctatcga
     1501 taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg ggaagttact
     1561 gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata agaagattag
     1621 tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac gagatagagc
     1681 gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta atcccagtca
     1741 agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg gccaacctta
     1801 cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt cttctgatcg
     1861 aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt ttgtcacgcc
     1921 taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag ctcctacatt
     1981 tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc aaagaagtct
     2041 tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc tcattaggca
     2101 gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt tttttcaaga
     2161 aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg atgtaaaagt
     2221 caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga taactatcag
     2281 aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga ctagtatcga
     2341 cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc aaattgatgc
     2401 caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt attttattgc
     2461 tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc ctgaaaaagt
     2521 actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat cgaatgaaat
     2581 aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata aagataaaga
     2641 cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag agaagtataa
     2701 agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga atacagaagg
     2761 catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca aactttatct
     2821 ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta gaccattaag
     2881 tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt ttggaagttg
     2941 gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc ttccagaagc
     3001 tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg ttacgatttt
     3061 cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta cagctcatcg
     3121 tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg aggggaaact
     3181 atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata taacaaaatt
     3241 attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga gacaagattt
     3301 tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc aaaacaacaa
     3361 ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc agtgtcagct
     3421 agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga caagagaaaa
     3481 acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc ggaattgaga
     3541 aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac caccaattca
     3601 aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct gatctttgat
     3661 ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag tgtccaaacc
     3721 gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc tggtgggatc
     3781 acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca aagcttatcg
     3841 attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact gaacgaagtt
     3901 gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag aaatgtcatc
     3961 ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat tgaaacaaaa
     4021 attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg attgcagatt
     4081 gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct aaccccaggt
     4141 gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa attttcgaga
     4201 aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa agattttaca
     4261 atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa aaaagacaat
     4321 aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt atgcatactt
     4381 ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa caaatagtaa
     4441 atttttatta ttgtaggatg gtt
//
LOCUS       DAZUKR010000169         4439 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000169 DAZUKR010000000
VERSION     DAZUKR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.172"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..1650)
                     /locus_tag="ACG89C_002603"
     CDS             complement(7..1650)
                     /locus_tag="ACG89C_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4901095.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            complement(1647..2006)
                     /locus_tag="ACG89C_002604"
     CDS             complement(1647..2006)
                     /locus_tag="ACG89C_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901096.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            complement(2167..2427)
                     /locus_tag="ACG89C_002605"
     CDS             complement(2167..2427)
                     /locus_tag="ACG89C_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901097.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2418..2729)
                     /locus_tag="ACG89C_002606"
     CDS             complement(2418..2729)
                     /locus_tag="ACG89C_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4901098.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(2730..3302)
                     /locus_tag="ACG89C_002607"
     CDS             complement(2730..3302)
                     /locus_tag="ACG89C_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901099.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3367..3594)
                     /locus_tag="ACG89C_002608"
     CDS             complement(3367..3594)
                     /locus_tag="ACG89C_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901100.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            complement(3635..3895)
                     /locus_tag="ACG89C_002609"
     CDS             complement(3635..3895)
                     /locus_tag="ACG89C_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFS4901101.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            complement(3885..4073)
                     /locus_tag="ACG89C_002610"
     CDS             complement(3885..4073)
                     /locus_tag="ACG89C_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901102.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
BASE COUNT         1299 a          950 c          631 g         1559 t
ORIGIN      
        1 tataccttaa aattcaatct catccaacat atcaaatgct tcctcataat ccatatcagt
       61 tatttcttct cgtttatata aagctgccaa aaaagcatga aacccatctg ttttacgcct
      121 tactggctct tttttcaaaa atgttttatt ccctaatttg tcttctttga taaacgaatt
      181 atttgtatac caacgcatca taggatcatc accaaaaata aaccgttcat ttgcaaaacc
      241 atcttcaata ataggagcaa cttttgactg cacacctctt atatttctga taaactcaaa
      301 attatatgat tctttctcta gaagaggttt cagcaaatcc atacgaaatc cgtcggcaca
      361 aacgatttct atttgatgga tttttctttt ttcatttaac caatcaatta aatgacgagg
      421 atcaattgat ggctcatcaa cgattgtcac taaacctttt ttctcccaat ccttaatagg
      481 cgctttcagt ttgaatgtat ctagaaactt tttcctagca aaactatgtt gctgccagat
      541 aaatttatca tcaattttaa aaagcaaacc cactgttgca aaatcacgaa tacttgcata
      601 atcaaatcca gccacacaag aacgaccatt caaatttggc aattcttgtt tagtagccat
      661 taatttttca tgagtcgtaa tatctgattc tgcatcacct tctgtaaaat tcattcgttt
      721 aacaacaaat tcacgcctac ctgatggctc atcttctagt tttttatatt ctttcatcac
      781 tgtagaaaac agacgttttg aatatgctga atcttcttcg aacattggat tagcctttga
      841 ccacatttta ggattatcca tttcatcaat gttatccagt ttacaaataa agggaaacaa
      901 gcgatcattc ttgttctctc cactaaaaat cttttgtgct cgttccaaca ttttgtcata
      961 aaaaccttct ctaacatgac catttgtacc attataaaaa gttcgaggat gttttatttt
     1021 ccctagccca ctacgctgaa tatcaactag gtctgaagat tcaaactgat ggatttcatc
     1081 aaattctaag cagccatctc ttgcactatc catagtctta ggattgtttg tcctataact
     1141 aaaaatcgaa ttagttacat tgttgactat agccatctta gtcaaataaa attgtttttc
     1201 cagtttgcgc atttgtacca tttcataaac ttctttaaaa gatacctttc cctgcttctc
     1261 agaattcgct gtaatcgtta catcataatt tctgacaccg tagagagggc taataaaaaa
     1321 tgagtctctt gctgacatga acccattttt cccgccgcct cgcgcaatac tatttagaat
     1381 ttcatcaaaa aacacctcgt cgtcctcttt ttgatagcag aaaatgaaag gtgtgataaa
     1441 tttttgatat ttagctaaag gaaaaaaatt tttttcagtg tacttgataa acttttgaat
     1501 catttcttca tcaaaataga tatcatcccg aactagcact tcgtttttta aataagtaat
     1561 taactgaatt ctttctttat taaaagggat tacccctttt tcgtataaat ctacgtattc
     1621 atcaaaatag aatggcttta atgttgtcac agcaaatcac taccatctag cgctgcattt
     1681 tttgtaggtt cgcgcttttc ttctggcaaa tagtcagtaa gttgcttaat tacacgttga
     1741 tatgcttggt ctctcgaatt atactgttta gcaactggtc gttcccgctc gtaaggtttt
     1801 tgattttctg actgagagaa aagctcataa tcaccttttt cggaaatatc aatccacatt
     1861 tcatcaagaa gaatttttaa gcgtgccgct tgcgtaatca aaccagaaac gaccttcttt
     1921 ttatccttat caatatcctt aaacaattta tttagacgcc tttcttcagc agaaacaagc
     1981 ctatttctct cttgcagatc agccatcaac tcacttcctt tctacgtggg gagggggtcg
     2041 tgtgaaaata gcatcattta tttgcagagt tgaccccatc caccggttcc caaaaatttt
     2101 atttcttctc aaaatctttt gaccgggggt atttcactct atatatcttc gctttatact
     2161 gatttatcag ccgtcttgct cgttgctttc tacaatatgt gcaaatggac tcaaggtata
     2221 atcaatgtct acattaatat taaatgtccc tgttacttca ttagcgttac ctattagtct
     2281 attgttgata gatatatcct ctacagttgt tgttccttca atgagctgac tcttaaatag
     2341 aaccaactta tcaatagcct tatcaaccat agcgattgct tctgctcgtt tgacatcatc
     2401 tctgtttgct gtttttctta ccaccattca tcatcccact ttcttttctt ttgttggcca
     2461 cgatatttca tacggttgtg ccttttgtta tggcaatctt tgcataaggt tcttaggttg
     2521 tttatatcta gagctaaatc cggatgatac tccagctcct ttatatgatc cacttcaaga
     2581 attgaatcat gttgagttgt tagtctaccc tctgctttac accattggca ctcataatga
     2641 tctcgctcta agcattgact tctaagtttc ctccactcag ttgatccata gaacctagct
     2701 cttgcttgtt tacttgaaac atcaatcatt tacatatctc tcttgtgccc aatgattttt
     2761 ctatctaagt acttgttatt atgtgaatca taatattcaa tagtgatgtc gttagctcct
     2821 tttggtactt ggcattcata ttcttccgta tcccaatgat aagtaatatc gacaagacct
     2881 atcggcatct catccattcg ttgaccttta taataaactt ctggtactga gtcagtatct
     2941 cttagtttga tttctagaag gttcgtatta cttccatcat ctaacgtcaa ttcagcaatc
     3001 ctctttttga tctgttgaat tacttttctt cctgtctcac ctgtcattgg tactaccgtc
     3061 tcatatccag taaactcgca agtaaagcgt tcaatagcat aagcgttttg aagtggctta
     3121 gcactaagaa cagctggatt attgctataa aaacgatcta ctaataactt ccctatagga
     3181 atggcatcta aagctctatc agtaaacact aaagcattgg gataatcttc ttgaattttg
     3241 taagctagat tgggtgcagt tacaacgtta tacccttctc tgatataatc ttttaatttc
     3301 atagttttcc tcctaaaaac atctaataat tacttgctaa caacttgatg taatcatagt
     3361 tatcattcat ttgatgtacc tctcaatgtt gtattggata tactcgtctt tccaatagcc
     3421 atgaccgcaa tatatcagct tgcatttatc cacttcgttt ggtgtagctt ctcttagcat
     3481 ttcgacaata gagtacttcc ctttgatttg tacagaacgc acaacacgca ctgaacaatc
     3541 accaatggtt cgaggatatt cattagttag tgatacatac cagtagtttc tcattatgca
     3601 gcctccttta tgtaaagaaa aagcctagca cctgctagac tttgatacat tcatttgatt
     3661 ttttgagata agtatctaca tagatttcac ctttgtcccc gttcaacgta atttcaaaat
     3721 acggtgctcc ttttaagcta gcgctgacta atgctttgct attttgcagt gttttgcatg
     3781 accagactat gaaaatacta aactcaggca ctgctccact taggtcagca atctcatttt
     3841 caattctttc acgtacaata gccttgcatt tttcgataaa cttctcatga ttcattttgt
     3901 acctccgcaa gcgcataacc agctgttgat gtattgttaa acgtagctga acgagtaact
     3961 cccgtagcct tcccagtata aataaaacta aatcctgttg ttgttggctt aaaattctcc
     4021 acttcataaa aatgatatgt ttgtccgtta ttagtaaata caatcaattc catttttcaa
     4081 aatcctctct tttttaaaat aaaaagacca ctcaaagagt gatctaatat gtaatgcaca
     4141 ggcagggacg tttccgatcc tgtgcttgag tcatttgacg attcatttgt accgaatccc
     4201 aaaactcaat ctaacccaac caattatgta atagcaacct acaagatgca caaaacgcgt
     4261 acgaaattgc gcacccctat atttttaaac cgccgatgcc tcggttacca aagtcactgg
     4321 caaggaatcg aaccttgcag tttgcaccta aattttagat gagcgataca cctttccacc
     4381 acagtgacat aatgacaata gacagcaact gatgatagat aataagaaca atttagaag
//
LOCUS       DAZUKR010000170         4434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000170 DAZUKR010000000
VERSION     DAZUKR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.173"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG89C_002611"
     CDS             complement(543..2048)
                     /locus_tag="ACG89C_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4901103.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG89C_002612"
     CDS             complement(2045..2731)
                     /locus_tag="ACG89C_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4901104.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG89C_002613"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG89C_002613"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4901105.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAZUKR010000171         4425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000171 DAZUKR010000000
VERSION     DAZUKR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.174"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG89C_002614"
     CDS             690..1658
                     /locus_tag="ACG89C_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4901106.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACG89C_002615"
     CDS             1726..2484
                     /locus_tag="ACG89C_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS4901107.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG89C_002616"
     CDS             2552..3262
                     /locus_tag="ACG89C_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4901108.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG89C_002617"
     CDS             3298..>4425
                     /locus_tag="ACG89C_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4901109.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZUKR010000172         4396 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000172 DAZUKR010000000
VERSION     DAZUKR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.175"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..93
                     /locus_tag="ACG89C_002618"
     CDS             <1..93
                     /locus_tag="ACG89C_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4901110.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="ACG89C_002619"
     CDS             complement(151..1371)
                     /locus_tag="ACG89C_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4901111.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="ACG89C_002620"
     CDS             complement(1391..2002)
                     /locus_tag="ACG89C_002620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4901112.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="ACG89C_002621"
     CDS             complement(2045..2977)
                     /locus_tag="ACG89C_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS4901113.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="ACG89C_002622"
     CDS             complement(3398..4258)
                     /locus_tag="ACG89C_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4901114.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DAZUKR010000173         4324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000173 DAZUKR010000000
VERSION     DAZUKR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.176"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG89C_002623"
     CDS             complement(<1..281)
                     /locus_tag="ACG89C_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4901115.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="ACG89C_002624"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="ACG89C_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="ACG89C_002625"
     CDS             complement(2359..2721)
                     /locus_tag="ACG89C_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901116.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="ACG89C_002626"
     CDS             complement(2725..3033)
                     /locus_tag="ACG89C_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901117.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="ACG89C_002627"
     CDS             complement(3042..3461)
                     /locus_tag="ACG89C_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901118.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="ACG89C_002628"
     CDS             complement(3454..3687)
                     /locus_tag="ACG89C_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901119.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="ACG89C_002629"
     CDS             complement(3684..>4324)
                     /locus_tag="ACG89C_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4901120.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DAZUKR010000174         4315 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000174 DAZUKR010000000
VERSION     DAZUKR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.177"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..899
                     /gene="recO"
                     /locus_tag="ACG89C_002630"
     CDS             87..899
                     /gene="recO"
                     /locus_tag="ACG89C_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4901121.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            1283..2200
                     /gene="glyQ"
                     /locus_tag="ACG89C_002631"
     CDS             1283..2200
                     /gene="glyQ"
                     /locus_tag="ACG89C_002631"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4901122.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            2202..4277
                     /gene="glyS"
                     /locus_tag="ACG89C_002632"
     CDS             2202..4277
                     /gene="glyS"
                     /locus_tag="ACG89C_002632"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4901123.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         1462 a          794 c          933 g         1126 t
ORIGIN      
        1 ttgagaaaaa cggcggaaga tcgaaacata aataatctgg atcggcagaa tctgcttttc
       61 cagatttatt ttttaggagg gagaatatga gccagcttgc agaatcgaaa ggcttgattt
      121 tatctagccg taattacaaa gaaaaagata aattagtcaa aatctttacg gaatcttctg
      181 gcaaaatgat gttttatatc aaaggaatcc atcggcaaaa tcagccatta gctccggctt
      241 tgctcccctt taccgaagca gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt
      301 taagcagtgt caaagacgtc catgcgtttc ggaacatcca agaagatatc tttttgagcg
      361 gttatggtac ctatttgcta aatctagtag atgcagcaat cgaagatcat caatatgacc
      421 cgcatttatt ccaatttacg caacaagctc tgcagcggat ggatcaagga gatgatgcag
      481 aaatcatcac gaatatcttt gaagttcaaa tactgcaacg cttcggaatc gcgccaattt
      541 ggacgcactg tgtcgtatgc ggggaaacaa aaggaaaatt tgactattct tccaaatctg
      601 gcggtgtgat atgcgagaaa cactggccaa tggacgaaca ccgttatcat gcagatcctc
      661 gcgctgttta tttcgttcga atgttttcgg ctatttctta tgataagatc agtggaatcc
      721 acttgaaaga agaaacgaag caggcaatcc gtgacctcat tgaccagttg tatgatgaat
      781 atgtcggtat tcatctaaaa agcaaaaagt tcattgatca aatgaagtct tgggcacacg
      841 tattaaagac gccagagaaa aatatgcctg aaaaagagat acctgaaaaa gaagaataaa
      901 tgaaagatac aacaaaaaaa tcaaaactat ttgacaaata cagggacttc cgtctattat
      961 taaccgtaga ttaaatagat cgttcactga gaggatctat tatataaaca agaaaactgc
     1021 aaaatgaata cgtacattga ccaagatcag taaaaaacac gtggtttagc gagttcggga
     1081 gagtggaagc cggacccaaa cgatttttga agaggcactt gcgagtacga taaatgaagc
     1141 tgccgaatct ttattcggaa gtagggtgga accgcgaaat tattcgtccc tatgtctgta
     1201 atcagacata gggacttttt gtcgttttct ttcctgtatt cttattgaca gcaacgctta
     1261 aaaattgaaa ggggcaaaac agatgagtaa aaaattgacc gtccaagata tgattttaac
     1321 attacaaaaa ttttggtcat caaacggctg catgctgatg caagcttatg acacagaaaa
     1381 aggagcaggt acgatgagcc catatacatt cttacgggca atcggaccgg aaccatggaa
     1441 tgctgcttat gtagagccat cccgccgacc agctgacggg cgttatggag aaaacccaaa
     1501 ccgtttgtat cagcatcatc aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca
     1561 agagctttat ttagaaagct tgcgtctttt aggaattgat ccattagaac acgatattcg
     1621 ttttgttgaa gacaactggg agaacccatc gatggggtgt gccggtgtgg gttgggaagt
     1681 atggcttgat gggatggaga tcacacaatt cacgtacttc cagcaagtcg gaggactacc
     1741 ttgtaaacca gttacttctg agatcactta tggactagaa cgtcttgctt catatattca
     1801 agaagttgaa agtgtctatg atctggaatg ggctgatggt gtgaaatatg gcgagatctt
     1861 caaacaacca gaatatgaac attccaaata ttcttttgaa gtaagtgacc aagacttgct
     1921 actaagcaac tttgaccgtt ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt
     1981 tcatccagcg tatgattata ttttgaaatg cagccataca ttcaatttat tagatgctcg
     2041 cggtgcagta tccgtaacag aacgagcagg ttatctttcc cgtatccgca acatggctcg
     2101 tgctgtagcc aaaatctttg tggcagaacg cgaaaaatta ggattccctt tattgaataa
     2161 aaacacagca aatccagaaa acatgaagga ggctgaataa gatggcaaaa gacttattac
     2221 ttgaaatcgg gctggaagaa atgcctgctc atgttgtcac accaagcatg aaacaattag
     2281 aagaaaaaac agcaaaattt ttagatgaaa accaattgac ttatgatacg atcgaaacgt
     2341 tttctacacc gcgccgctta gcaatcaaag taacaaacat tccagaacgt caagcagaca
     2401 gtgaagaaga agtcaaagga ccagccaaaa aaattgcttt ggacgcagat ggaaactgga
     2461 cgaaagcagc tgaaggattt gtccgcggac aaggtctgac gacagatgct atcactttta
     2521 gagaattaaa aggcgtggaa tatgtctatg taacgaaata cactcacggc aaagaagcga
     2581 aagaagtatt gacaggttta gcagatgtca ttaaaagcct cactttccca gtaacgatgc
     2641 attgggcaga atacgatttt gaatatatcc gccctattca ctggatcgtg gcattgttag
     2701 atgacgaagt aatcccgttt gagatcttag atgtcaaaac aggcagagcc tctcgaggac
     2761 atcgtttctt aggcgaagat gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga
     2821 atgaacaatt cgttattgct tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa
     2881 atgaattagc cgaaaaaaat cactgggtta ttgatctaga cgaagattta ctagaagaag
     2941 tcaacaattt agtcgaatat ccaactgtct tttctggcga cttcgaagaa aaatatcttt
     3001 ctgttcctga agaagtacta gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac
     3061 ggaacgatca aggtatgctg cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa
     3121 aattggaaaa tgtggttcga ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg
     3181 aattctttta taatgaagac aagaaagctt caatcgacca atacgttgaa aaactaaaaa
     3241 ctgttacttt ccatgaaaaa atcggtagta tctatgagaa aatgcagcgg gtacaagtca
     3301 ttgcccgtat cattggcgaa caagtaggac tatctgagga agaattgatg gatctgcaac
     3361 gagctgctga aatctataaa tttgatctag taaccaacat ggtaggggaa tttcctgaac
     3421 tacaagggat catgggcgaa aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa
     3481 cagcgattcg tgagcattac atgcctatat ccagtgaagg agagctacct cagtctacag
     3541 taggttctgt tttagcgatt gcagataaat tagatagcat tttttctttc tttgcagttg
     3601 ggatgattcc aagcgggtca aacgatcctt atgccttacg tcgccaagca tacggtgtcg
     3661 ttcggatcat cgaatcgaaa aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta
     3721 gtgatgttat cagtaaagat acggatcgtt tcggcattgg tttatcagaa gggcaacaac
     3781 aggtgatcga ctttatcaaa ggccgtctgc gccaattatt gacaacgaag aatattcgtc
     3841 atgatgtcat cgaagctgtt ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag
     3901 cagcacaatt gttcaagcag catcttgcag atgaagactt caaaccatcg atggaagctt
     3961 tgacacgtgt cgtgaactta gcgaaaaaag cggagcttga aaaacaatca gaagtagacc
     4021 ctgaactgtt cgaaaatgaa gcggagaaag aactgcacaa agcagtagag gcaatgaaaa
     4081 aaagctttgc tgaccaaacg atcaacgaaa attatgatca gcttgtaagc cttcgcccac
     4141 tgatcgaaaa ttattttgat cagacgatgg tcatggcaga agaaccagca gtacgtaaga
     4201 accgattaac gcaattaagt caaattgctg ccatggcttt gtcgctagca agtttagatc
     4261 aaatcatcac aaaataattt ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DAZUKR010000175         4173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000175 DAZUKR010000000
VERSION     DAZUKR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.178"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(466..969)
                     /locus_tag="ACG89C_002633"
     CDS             complement(466..969)
                     /locus_tag="ACG89C_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4901124.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1328..2353
                     /locus_tag="ACG89C_002634"
     CDS             1328..2353
                     /locus_tag="ACG89C_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4901125.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2676..3554
                     /locus_tag="ACG89C_002635"
     CDS             2676..3554
                     /locus_tag="ACG89C_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS4901126.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4030..4146
                     /locus_tag="ACG89C_002636"
     CDS             4030..4146
                     /locus_tag="ACG89C_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4901127.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1386 a          662 c          782 g         1343 t
ORIGIN      
        1 aataccgtgt ctcagatttc tctactactt ctaaaaacta ctgttttttt cctttgaaat
       61 gccttcaaaa agtaggatca ggcagaatat actggcagaa tcaaggagaa gggagcctaa
      121 ataaggagaa aaaccataga aaatataaag taattactta taaaacaagc tttttataga
      181 aaaataatat acctgagtag ggtataaatt aagacggaat tcctttctag attgagatat
      241 tgactaggaa gaatgttaag attttaagcc tttaagctaa aaaaaaaccc tttttgttaa
      301 ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt
      361 agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga
      421 tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac
      481 agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat
      541 aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca
      601 taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat
      661 accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt
      721 tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac
      781 aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa
      841 agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag
      901 cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac
      961 ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca
     1021 taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt
     1081 ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg aaaagttgct
     1141 atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt ctccaagttg
     1201 tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac taggtaatgc
     1261 tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac taggaaggag
     1321 ggatagattg ttcaagactt attttcgttt caataaaata caagataatc cttttcgtat
     1381 catgaatctg atgctactta tctttattgt attatggtta ggttacataa atcatgattt
     1441 tttgatttca agtttttcat cattagggat ctttactttt ttctattatc aaaacatacc
     1501 gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg ttgctttcgt
     1561 tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag ctttggttgc
     1621 tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg gcttgttttt
     1681 tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat taccgatcgt
     1741 ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt
     1801 actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa gatttcacga
     1861 agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc tttccgttta
     1921 tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat gtgcatcgat
     1981 tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc tgattggatc
     2041 gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc agttgcagac
     2101 catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta gaaattatgc
     2161 agttgctaat atttttctta atccgatggc gttgatgcta tctacactta ttcgtggagc
     2221 atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa gtttcatcgg
     2281 tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg ttgttcgtca
     2341 aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag atttttgtca
     2401 caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa attcacacaa
     2461 aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa attttgttta
     2521 tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga
     2581 tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat
     2641 accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg
     2701 gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc
     2761 ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg
     2821 agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag
     2881 acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc
     2941 aaagttggaa aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa
     3001 tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg
     3061 accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg
     3121 agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt
     3181 gcctttctct tcatattcct tccgatgcag atttatctca gaataagata agagatagtt
     3241 acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt
     3301 acacagatac ttggttacta tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa
     3361 tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag
     3421 tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt
     3481 tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta
     3541 ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa
     3601 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
     3661 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
     3721 cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac
     3781 gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa gagggccttt
     3841 tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta
     3901 gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca
     3961 caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg
     4021 gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat
     4081 atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa
     4141 aggtaagaag aaggaaactt aattaaaaaa ata
//
LOCUS       DAZUKR010000176         4036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000176 DAZUKR010000000
VERSION     DAZUKR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.180"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..391)
                     /locus_tag="ACG89C_002637"
     CDS             complement(242..391)
                     /locus_tag="ACG89C_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901128.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ACG89C_002638"
     CDS             853..1065
                     /locus_tag="ACG89C_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901129.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ACG89C_002639"
     CDS             complement(1343..3142)
                     /locus_tag="ACG89C_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS4901130.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ACG89C_002640"
     CDS             complement(3266..3862)
                     /locus_tag="ACG89C_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4901131.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DAZUKR010000177         3876 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000177 DAZUKR010000000
VERSION     DAZUKR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.181"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACG89C_002641"
     CDS             158..2257
                     /locus_tag="ACG89C_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS4901132.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACG89C_002642"
     CDS             2453..3316
                     /locus_tag="ACG89C_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS4901133.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="ACG89C_002643"
     CDS             3421..>3876
                     /locus_tag="ACG89C_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4901134.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DAZUKR010000178         3811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000178 DAZUKR010000000
VERSION     DAZUKR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.182"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..490)
                     /locus_tag="ACG89C_002644"
     CDS             complement(<1..490)
                     /locus_tag="ACG89C_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4901135.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVM"
     gene            543..1019
                     /locus_tag="ACG89C_002645"
     CDS             543..1019
                     /locus_tag="ACG89C_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901136.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(1025..1207)
                     /locus_tag="ACG89C_002646"
     CDS             complement(1025..1207)
                     /locus_tag="ACG89C_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901137.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(1207..1506)
                     /locus_tag="ACG89C_002647"
     CDS             complement(1207..1506)
                     /locus_tag="ACG89C_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901138.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(1611..1814)
                     /locus_tag="ACG89C_002648"
     CDS             complement(1611..1814)
                     /locus_tag="ACG89C_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901139.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1829..2575)
                     /locus_tag="ACG89C_002649"
     CDS             complement(1829..2575)
                     /locus_tag="ACG89C_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4901140.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2653..2961
                     /locus_tag="ACG89C_002650"
     CDS             2653..2961
                     /locus_tag="ACG89C_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901141.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(2932..3189)
                     /locus_tag="ACG89C_002651"
     CDS             complement(2932..3189)
                     /locus_tag="ACG89C_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFS4901142.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(3487..3627)
                     /locus_tag="ACG89C_002652"
     CDS             complement(3487..3627)
                     /locus_tag="ACG89C_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901143.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
BASE COUNT         1057 a          728 c          597 g         1429 t
ORIGIN      
        1 ccataacttt catatttcct ttttgcttaa tttcaaactc ttgttgtccg ctaattcgca
       61 tagtatcttt aagcatggaa acatcttttt taacatcttt catttcttct aattgataga
      121 tcatcatgtc ttcaattgtt tgaggaacag tattcttccg aataacatct tccatttcgt
      181 tgaaagcttc aataaatttt agtttgaaat ttattgcttt acttccagtg aatcccatag
      241 ctagcaagga aaatccgtct ctattcatga aataaactct ccgacttctt ccgtatgagt
      301 ctggctcgtt cccttccaca aacatctgtc caaaattgga cccatcttca acattggccg
      361 aattttcgac cgatcttttt attgattcaa ttgcttctag tacatgctta tgtttctttt
      421 tgaaactttc tgccacttgt aagctcgtag ttacagcttc tttatttttc aaaattacta
      481 attcttgcat tatttcttct ctcctttttg gtataatttt cgttagaaag tgaggtgatt
      541 ctatgaggaa aaagctcaat tacattctta ctgcgacttt aaccatatat ataattctgt
      601 ttattacctt atctttcctg tcttcaatta aaaaaattcc ttttattaca ttgaataaaa
      661 atagtatttt gaaggagttc aataacactg actacatcgc atttctaaaa cttttttcag
      721 tctgtgtgct aatatcatgt gtaattattt ttattgttgc taaaacatgt tggacaatgt
      781 taaataaaaa ccaacgcgat gttgaaaata ttgctatatt tttaattaag attacttcct
      841 caatatcaat tatcattccg atgactgtaa ctttttcaaa agaacagttt aatgtagcgg
      901 ctacttatct ttcatttata gcgctatttt cttttgttct tcctaaaaga aataagagca
      961 actttgacaa caaggagctg gatgaagtac gcaatagcag cccaaaggaa caaaaataaa
     1021 aaaatcatat tttagtcagt ccctcccgac tggctttttt cgctctgtgc tcagcttcat
     1081 caagccccat aaaaatccaa accatgtaaa cgattgtccc tatcaacgct tgtctgcttc
     1141 cccagaggcc taaagcgtag atgattagcg gtgcgctgaa tactagtgct ctgttgaatt
     1201 tgcccattaa cttgcctcct tcaaaacatc tgaaggcgat aaacttttta cacgatattt
     1261 gtttgcgtct ttccacttta aaaaccaaat aaaagtatgg taatgaataa atgtggttga
     1321 gtgacctgga cgaagtattc cttctgcaaa ttcaggaatt gtttccattt ccttgcagta
     1381 ttcaactaaa gtagtttttg acatgccgtg gaacttctcc aagattaaac tttgtcggta
     1441 ccagtcgtct ggttttactg ccttctcagc agcttctatc agctctgaaa gtgttggttt
     1501 tttcacttat tatccctcct aaaaattatc gttccatact cgatgtttaa catttcttga
     1561 agctctgatt ttgtaagaat gattttatct tccaacgact ttacctccct ctacaattcg
     1621 tacatagtga taatcgaatc tataattctg tttgcttctg cagaagtctt tttaccgttt
     1681 aaaattaaag ataagtagct tttgctaatt ccaaatcttt cagcaagcat ggtgtaagtt
     1741 aagaactttg aactttcgac atattctttg attttttctc tatctcgttg agtgatttct
     1801 gcaatgtcag tcatactaaa actcctttct aaccaatttc ctctaaatcc atttgagggt
     1861 aatagccctc tgcgattaat aagttgtaaa taaacactcg ccctttctgt gtccatttgg
     1921 tattcattac aactttagtg ccgccatcgg ctttcggtat ctcacttgtg tgcgattttg
     1981 tgtatccttg gttcatatgt tttcggtaaa gtatccattg tccgctaact ttatgctgaa
     2041 cacctaactc gtttagcaat ttgtttagag caattgccga cattccgtaa tcagctgcaa
     2101 tttgagaagt agctaccgta tctgtcgatg aaagaatcgt atctaagtag ctaatctttg
     2161 gttcgtactc ggcaatttgt tgttcgagta attggttctt ttcttctaaa tccgctgcta
     2221 aacgcaatgc ttgtgcaaaa ctttgaggta cgtttgaata gctacctgtt tttctaattg
     2281 ttggaaggac ttcactcgtt acccaacgtt taaatttttt ggcagaggga agttttgatt
     2341 ttaagattaa actgtaaagg cctgactcgt taatcaaaac aactgctgtt ccattgacgg
     2401 tgaacgaatc gttctgcgtc ttatcctcca agtccacatg atctcgaata gctttttgcg
     2461 gattcgaata tcctagaacg tctgctacat cttttcctac aaaatatggt tcatcattta
     2521 ctaaaatagt tcgaacttcg ttttgctcga aattaaaaat ttgtggtgta ttcatatttc
     2581 tcattccttt ctttggtata atttccttat cagtgtgaca ggctgaaata attgttaagg
     2641 aggtgaaaat ttatgaaacc agaatttgta attactatgc ttgatggaga aaaaatcaaa
     2701 atatttgccg atactttggt cgttggatac gatgatgcgt caaatatcga agaaaaagat
     2761 ggaatgttct attcttctca aatttacgtg aataccttgc aaggtgatat tggggtatct
     2821 actcttgcaa catcaagacc gcttgtaggt cttatcggct ttttgtcaaa agcaacattc
     2881 ttttctctcg gcgatgatat agattcggat gaaactgtca tctataaaac atcagctgtt
     2941 aaaagtatcg gtttcaaata gtaattcttg ctcgttgata gcggaagcca acttttcttc
     3001 caacacatcg gcgagctttc tttttgttgg attcatttca aagttgatag tcttgagaag
     3061 catttttgca tccttaactt tttgtccttg caattccttg atgattttat cggcttggct
     3121 atcaattcca tgtgttagat catctatcat tttttgtacc ttgattgttt ggctcatttc
     3181 cttgttcatt gtttagcctc ctttaataaa tgattttttg tattttttaa tttccataaa
     3241 ttcctcaaat tgctctttgg tattattacc acgaccatac atattgtgaa attctttatg
     3301 gcaactgaca caaagtgtaa cgccgtttga aatcgctgcg gatttagttg ttgcatggct
     3361 aaatcacgaa tcacaagtat ctactaaagg cgaatcaatt tcgccaaaag aaattgttca
     3421 agcttattta gatattcatt atgctgttat ctatggtcag cttccagaag accgtaaaaa
     3481 cgatgactaa acgtgagcta aaatctctgc tatggctgca accatggcag agtcttcttt
     3541 atctaccgcg tgttccatcg ccacttttgc ttgtttcagt atgtctaatt tcaaatcttc
     3601 gatttttgcc gagactgttt tttccatatt cttctctcct ttcttttata ttcgtaaaca
     3661 aatttaacaa ctttttaaaa aactgcgttg actttattag attaataatc tatactaaat
     3721 gcatagttaa ataagacata taaactttga tttaaaaagc tttcttggcg gttggcattt
     3781 attaatcaat agtatttttt gttgtctttt t
//
LOCUS       DAZUKR010000179         3787 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000179 DAZUKR010000000
VERSION     DAZUKR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.183"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1312
                     /locus_tag="ACG89C_002653"
     CDS             <1..1312
                     /locus_tag="ACG89C_002653"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS4901144.1"
                     /translation="YLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQ
                     SKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFY
                     TPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNT
                     TTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTT
                     FLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQK
                     LLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLS
                     EENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGN
                     DIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1302..2519
                     /locus_tag="ACG89C_002654"
     CDS             1302..2519
                     /locus_tag="ACG89C_002654"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4901145.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2532..>3787
                     /locus_tag="ACG89C_002655"
     CDS             2532..>3787
                     /locus_tag="ACG89C_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4901146.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1307 a          651 c          790 g         1039 t
ORIGIN      
        1 gtatcttttc aacgtcttag cggatcaagc aaaacaagca acgttccaat tgaacgatct
       61 aaacaaagcc tttgtgcaat tggcatctac ctacaatcaa tttaatgggt tatttgatga
      121 tgtggatctc caatcaaaaa aactaggaac agatgagcaa caacgaaatg tgacgattac
      181 cgaagtcatc aagaaattga atgatgtaga tgttttagaa cacgatgggg acgtgatcgg
      241 ggatgcgtat gagtttctga tcagtcaatt tgcttcagaa gcagggaaga aagccggaga
      301 attttacaca cctcatatgg tttctgatat gatggcgcaa atcgtaacgc tcgatcaaaa
      361 ggaacgacga ttctttagtg tttttgatcc aacgatgggt tcaggttcat tgatgttaaa
      421 cgtacgtaac tacttaacac acccagataa cgttaaatat catgggcaag aactaaatac
      481 aacgacatac aatctagcaa aaatgaactt gattttacat ggtgtcgacg ccgaagaaat
      541 gaatctccgc aatggggata cgttgaataa ggactggcca acagatgaac cttacacatt
      601 tgatgcagta gtaatgaacc caccttattc ggctaattgg tcagcagata cgactttctt
      661 agatgattct cggtttaatc gttatgggaa attagcgcca aaatccaaag cagattttgc
      721 gtttcttctt catgggttct atcatttgaa ggaaacaggt accatggcca tcgtcttacc
      781 acacggagtt ttattccgtg gcgctgcaga aggggtcatc cgtcaaaaat tactagaaga
      841 tggcagtatt tatgctgtga tcggtatgcc tgcgaacttg ttctttggga catcgattcc
      901 aacgacggtg attgtgttga agaagaatcg tcaaactcga gatgtattat tcattgatgc
      961 tagtcgtgaa tttgttaaag ggaaaaatca aaataaacta tcggaagaaa acattcaaaa
     1021 aatcctcgaa acctacgctg aaagaaaaga tgtcgagaaa tacgctcatt tggcaacctt
     1081 tgatgaaatc aaagaaaatg actataactt aaatatccca cgctatgtag atactttcga
     1141 agaagaagaa ccaattgata tggttcatgt cggaaatgac atcaagaaga tacgacaaga
     1201 acagcaagtg ctagaaaaaa aacttttaga agctatctct tccctgcaaa caacacctga
     1261 aaatgaagcg tggttacaag gtgcgctgga ggtgttcaaa catgagcaat gatacacaac
     1321 cagaaatccg tttcccagga tttacagatg attgggaaca gaggaagttg ggggacttcg
     1381 ttgtagatta tgttgaaaag acaagtgtac aaaatcaatt tcctatgcta acttcatctc
     1441 aacaaaaagg gatagttttg caagaagact attttgcaaa tcggcaagta actacagaaa
     1501 ataatattgg ttactttgta ctacctagag gatatttcac gttccgtagt cgaagtgata
     1561 atgatgtgtt tgtgtttaac cgtaatgaca ttattgatag agggattata agctattttt
     1621 atcctgtatt tactttaaaa tctgctgatt ccgacttctt cttaagacgt attaacaatg
     1681 gaatccaacg acaattatct attcaagcag agggaacagg tcaacatgtt ctgtctctaa
     1741 aaaagtttaa aaatatagta gcaatgtttc ctagtgagga agaacaacaa aaaatcggca
     1801 ccttcttcaa acaacttgac gacacgatcg ctcttcatca gcgtaagtta gatttgctga
     1861 aggaaacgaa aaaaggattc ttacaaaaaa tgttccctga aaacggagca aaagtgccag
     1921 aaatccggtt tcctggtttt acggaagatt gggaagagcg taagttggga gaattaggta
     1981 aaactcaatc gggaattgga tttcctgatg ccgagcaagg tggttctgaa ggtactccat
     2041 tttttaaagt ttccgacatg aataacattg gtaatgagta tgaaatgaga aatgcaaatc
     2101 attatgtatc taatgagcaa attgagcgta aaaaatggaa gccaataaaa gatgtgccag
     2161 cgattatatt tgctaaagtt ggcgcagcaa taatgcttaa tagaaaacgt ttggtcacaa
     2221 gccctttctt aatagataac aataccatgg cttatttatt taataatact tgggatatct
     2281 attttggtaa aatattgttt gaaactataa atttgccaag atactcccaa gtaggggcat
     2341 tgccttctta taacagttct gatattgaga atatcagtgt taaggttcct gtaaaggatg
     2401 aacaacaaaa aatcggtact ttcttcaaac agctagatga tacgatcgct cttcatcaac
     2461 gtaagttaga tttactaaaa gaaacgaaaa aaagcttctt acaaaagatg tttgtctagg
     2521 agggaagaag aatgaaaatt agtcaccgtg aggaagcaga agtcgaagaa cagttgattc
     2581 gtgtacttgg agaagggcat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
     2641 acctttgggt caatttacgc caaaaaatta tctcaaataa tcaagcagaa ctgaatgatt
     2701 ttcctttgac cgataaagaa tttgaaacga tcaaaacgga attactgctg cgaacgaaaa
     2761 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
     2821 aacgggaaga ttcgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
     2881 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
     2941 aagcacggga tcgacgtttt gacgtcacct tattaatcaa tggattaccg attgtccaaa
     3001 tcgagctgaa acaagtaact gcaaaagatg gtgtttatca ggcatttaac caaatcaaaa
     3061 aatatgcaga agaaggtatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
     3121 tttccaatga acaaaccact cggtattttg caaatgcctt accgaaagac ttgcacccta
     3181 agtttttatt tagttggcga acgaaagaca atgaaaaagt agaaaactta tatgagtttt
     3241 gtaaacaagt gttaaatatc ccggatgctc atcgtttgat tgcggattat acaatcgtca
     3301 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagta catgcgattc
     3361 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
     3421 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
     3481 atcgaacgat catgttagtg gaccgcaaag atttagataa tcaaaccacc acagagttta
     3541 caaaatttgc ttcggaattt aatactggga tttcttccgg gaacgccaaa gcgaatagct
     3601 tgatcgttgg aacgggaagt gccaaagaat taagtgaaac cctgttggca gatgccaatg
     3661 caaatgttgt aatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     3721 aacaagaaga gaaaaaagga accaatcgct tccaaaaatt aatgggccaa catatcgtgt
     3781 ttgtagt
//
LOCUS       DAZUKR010000180         3714 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000180 DAZUKR010000000
VERSION     DAZUKR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.184"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG89C_002656"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG89C_002656"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4901147.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACG89C_002657"
     CDS             complement(2262..2513)
                     /locus_tag="ACG89C_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS4901148.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACG89C_002658"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACG89C_002658"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4901149.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACG89C_002659"
     CDS             3463..3681
                     /locus_tag="ACG89C_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901150.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1204 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caag
//
LOCUS       DAZUKR010000181         3700 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000181 DAZUKR010000000
VERSION     DAZUKR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.185"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..351)
                     /locus_tag="ACG89C_002660"
     CDS             complement(<1..351)
                     /locus_tag="ACG89C_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901151.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKI"
     gene            complement(649..1623)
                     /gene="sgrA"
                     /locus_tag="ACG89C_002661"
     CDS             complement(649..1623)
                     /gene="sgrA"
                     /locus_tag="ACG89C_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS4901152.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1819..2658)
                     /locus_tag="ACG89C_002662"
     CDS             complement(1819..2658)
                     /locus_tag="ACG89C_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4901153.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2843..>3700
                     /locus_tag="ACG89C_002663"
     CDS             2843..>3700
                     /locus_tag="ACG89C_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4901154.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIK"
BASE COUNT         1241 a          753 c          545 g         1161 t
ORIGIN      
        1 aattttttga ctaagatttt ctaaggattg ataagtacct ttgtaaagtg aatccagtaa
       61 aatataacca atcgttccct ctaaaagtat agaataaact ttactcagta aaaactgaga
      121 agaaaactcg acttttatca tcttgttttc tgaaagaaaa gataatttat attctggata
      181 cctttcgcaa aacgattctg cttgttgaat caccgaattc atcttagcca gggtgtagtt
      241 tagatctaaa gcgatatcct tcaccacata taatttctcg tctctttcat aacaatagtt
      301 caaaaattga tatacttgcc tttcattgat atcaataaat tcttcaaaca aagtacatac
      361 tcctttttat ctattattta gacttattcc gacagctaca tgaataacgt acaaatagat
      421 tcgttactga tttttcagag atactaccat gcgcagtgtg cattgtcaat tattcttgct
      481 cttattttcc atttgtgttc ctgttatttc ctgtttctca aatatgttag taaacaaaaa
      541 tagatctggc aaagctacaa cttcgccagg caatacagca ctctcgaatg aaatattcac
      601 tcaaaaatct atcgttttat tcaattttag atctgttgct tttattcttt atttcactcg
      661 atggaagaga gaacgaaaat agcttgctcc tacaatcaaa atgagtaaca cacccatcca
      721 tgtaattagc ttgttcaagg ttctactacc agtttctggt aaatcgcttt ttctattatc
      781 tgtttctttc gctttttctt gatgatcaat tccacgagaa acaaaatcag gctgttttag
      841 cacacttttt aggttgctcc ctgcatttct cttttgttcc ttagttggta tgataggtac
      901 caaatcaagt tcattattta cttttcctgt gctgctttcg ctgctcgtgc ttcctgtgct
      961 actctcacta ctcatgcttc ctgtgctact ttcactactc gtactctccg tactgctttc
     1021 gctgctcgtg cttcctgtgc tactttcact actcgtactc tccgtactgc tttcgctgct
     1081 cgtgttttct gtgctacttt cactgctcgt actctctgta ctgctttcac cgctcgtgtt
     1141 ttctgtactg ctttcactac ttgtgctatc cgtactgctt ggcggtccaa caactgtcca
     1201 actgcttggt ttcaaattct ccgtgatcgt catctgtcca tcataaaaaa caatcaaatc
     1261 aactttatct ttactcttga tctcatcaat cgatagttct gcagaaatca ttattgtata
     1321 ctcaatacca gcggtccatt gtacgtcttt taaataaact tccccaataa ctttttctga
     1381 acttccatta ccatcccaac catatgttag aacagcatgt acatcactag cattcacgct
     1441 tttgtttggt ttgactggaa tagtaatgac ttgttgtgtc atgtcataaa caatccgatc
     1501 agttttcaca gtaaatgaaa gattctcatt tgcccgttca tttgcagaag tactgattcc
     1561 aatcattgct gcaattatac tcagcccaag aataacaaat aatacggtcg ctgttttctt
     1621 catttctatc actctccata taattacctt ctatttgata aaggaattat ataaaatgaa
     1681 aatgatgaga acacgacagt cagttgtgaa aatctaacaa aagatgtcgt gtattcgctt
     1741 gaatttgttt actttttcgt ttaatcaatg acagacttaa aaaagcaaga ccgatatcag
     1801 ccttgctttc ttcatctttt attgataaac agcagtgtat ttcttcatcc gctttgtttt
     1861 atatgcatag agtaacaaac caacaatcaa gactaacaag aataatgtag ttgatataga
     1921 tgcttcagcc ccaaagtttc ccccagtaat tattgtattt tgcgattgga tcagagtgga
     1981 gaaaaaacct tgttcttgcg ttccgctgac tggtataccc aaaatagctc cttgtgtcca
     2041 attccaagta atatgagcac caatagccgt aaataaactt cctgtactgt agataagtgc
     2101 gctgaaaaag caacctgcta aaaagatatt gaaaactgcc agaaatgtca tgttaggatt
     2161 caatccgtgc aaaagagcaa ataatgcagc agatccaatc attgctatta acggaccaaa
     2221 tctaagcatc tgatgctgaa aaaagccacg gaataaaagc tcttctgatg cccccactgc
     2281 aataaataaa ataaaactcg ttatcagcgg cacaatgttt gcgtttcctg aatctaaccg
     2341 atacgccccc gtcaaatata atggactagt aataagcaga aacactccaa ttccaacaac
     2401 gagacctccc gctaaatacc cccagtcttt tcctcgaaca gaaacaccat aatcttctat
     2461 tctttttcct tcgaccttac tagcaagaaa taaagctagt aaaaaaacag ctaaatgtcc
     2521 gaccgtaccg gcaattgctc caatccattc actatttcca ataaacatat ccgcaagtgc
     2581 attattgaca agaattccta aaacaaaacc aactaagact attaaaacag atccaatcaa
     2641 ccgaataatc ctgatcatct ttcatccctc ctttatatta agtttaacaa cccttttctt
     2701 tcttgtctcg tgaaaactat gtaaatgttc tccatcatag agagaataga tttttacgaa
     2761 ctaagaatat ctacagcaac cttacgaaaa tgaaagactg tctccctaat tcttctgtta
     2821 gactgttttt aataaggaaa ggatgggaaa ttttttgaaa aaacaactac taattatgtg
     2881 cctttcagca tgtgcaattt tactattctc aggttgtagc aagtccaatg aaacgattgc
     2941 aacgtttaaa ggtggaacac ttacagttca ggatttttat gataacccaa aagttaaatc
     3001 aaataatcaa acactattaa aaaaaatgat tgtcttcaaa gcatttgatg atgtatacgg
     3061 aaaagagatt tcttcaaagg aagtaacaaa agaatataat gaacaaatca aaaaactcgg
     3121 tccaaactac aaggaacaat taaaagcagt tggccaaaca gaagagacat ataagaaact
     3181 ctttaaacag atgctggctt tccaatacgg tttgaaagcc aatgtaaaat taacagataa
     3241 agatctagac actgcttgga aagaattcta tccagaagtt agtacacaga tcattctatt
     3301 ttcgactaaa gaagaagcag ataaagcaaa aaaagaagca aatgaagggg aaaatttctc
     3361 caagcttgtt caagcttatg gaaaaaataa aacattaaag gaaactgatg gaaaaatgaa
     3421 ttttgattca accaatccag agattcctac tgaagtaaaa aaagcagcat ttaaactaaa
     3481 aaacggggaa gtttctgata tcattcctgt aacagatcca acgacttatc agcaaagcta
     3541 ttatctagta aaaatggtaa aaaaacagga taaaggttct aataaagaca aatacaaatc
     3601 tgagctagaa aaaattgcta ccgaagcaaa aactcttgat acggaattca tggataaaac
     3661 aatccgtaaa gtgatgaaaa aagacaatgt aactattaaa
//
LOCUS       DAZUKR010000182         3680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000182 DAZUKR010000000
VERSION     DAZUKR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.186"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..306)
                     /locus_tag="ACG89C_002664"
     CDS             complement(142..306)
                     /locus_tag="ACG89C_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901155.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            601..1929
                     /locus_tag="ACG89C_002665"
     CDS             601..1929
                     /locus_tag="ACG89C_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS4901156.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2131..2964
                     /locus_tag="ACG89C_002666"
     CDS             2131..2964
                     /locus_tag="ACG89C_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4901157.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2980..>3680
                     /locus_tag="ACG89C_002667"
     CDS             2980..>3680
                     /locus_tag="ACG89C_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS4901158.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQL"
BASE COUNT         1224 a          582 c          766 g         1108 t
ORIGIN      
        1 ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt ctttttcttt
       61 tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag
      121 agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata cgaaataaat
      181 cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga cattcccaaa
      241 gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag caatcaggtt
      301 tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa tgagtatttt
      361 gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac aggccatttt
      421 cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt atgacatttt
      481 tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata gaagagattg
      541 tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa ggatgattgg
      601 atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca agcaaatatc
      661 aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat
      721 gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac ggggaaagtg
      781 gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac attccgtcat
      841 aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta cacttttttc
      901 tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac aggaaaagag
      961 gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga tggcggccaa
     1021 tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg
     1081 gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa attactgcgt
     1141 aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga cgctattgaa
     1201 gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc attaggaaca
     1261 cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca tcttgaccga
     1321 ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga tgattatgtt
     1381 agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac agtagagatg
     1441 ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat ggaaactttc
     1501 aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta
     1561 ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc aatggatgat
     1621 ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg agaaagtctg
     1681 atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc aacaaaatgg
     1741 aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat
     1801 gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt gacgaaagag
     1861 caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc aatgggtgct
     1921 ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga gctggcttaa
     1981 gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg tgctataatt
     2041 ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg atgacgaagt
     2101 agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt atctgatctg
     2161 aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac ctatcgtata
     2221 aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa aggatttttt
     2281 gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc ggatacgtat
     2341 atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga gccgggaaaa
     2401 ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac agcgccacaa
     2461 tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc ttttgaagaa
     2521 gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt ggcagaagaa
     2581 acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg
     2641 aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct gtggagtggt
     2701 aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt ttcttatggg
     2761 aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc ggaattttta
     2821 gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact gccgatttac
     2881 cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg ttcccaagta
     2941 gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa
     3001 aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg
     3061 ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag aggcagtgaa
     3121 ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga gattaatcgt
     3181 ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta caataagttt
     3241 ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt caaaatagaa
     3301 tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc
     3361 attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag
     3421 attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga tagtaatcag
     3481 catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata tgactttttg
     3541 actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga gtacgggaat
     3601 gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa
     3661 atccgaatgc aagaacagct
//
LOCUS       DAZUKR010000183         3678 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000183 DAZUKR010000000
VERSION     DAZUKR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.187"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACG89C_002668"
     CDS             complement(133..717)
                     /locus_tag="ACG89C_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4901159.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACG89C_002669"
     CDS             complement(928..1131)
                     /locus_tag="ACG89C_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901160.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACG89C_002670"
     CDS             complement(1141..1563)
                     /locus_tag="ACG89C_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901161.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACG89C_002671"
     CDS             1801..2415
                     /locus_tag="ACG89C_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4901162.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACG89C_002672"
     CDS             complement(2580..3404)
                     /locus_tag="ACG89C_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901163.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ACG89C_002673"
     CDS             complement(3428..3583)
                     /locus_tag="ACG89C_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901164.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT         1221 a          638 c          522 g         1297 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagttta
//
LOCUS       DAZUKR010000184         3654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000184 DAZUKR010000000
VERSION     DAZUKR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.188"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..545
                     /locus_tag="ACG89C_002674"
     CDS             75..545
                     /locus_tag="ACG89C_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4901165.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
     gene            complement(465..683)
                     /locus_tag="ACG89C_002675"
     CDS             complement(465..683)
                     /locus_tag="ACG89C_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901166.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ACG89C_002676"
     CDS             790..1104
                     /locus_tag="ACG89C_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901167.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ACG89C_002677"
     CDS             1208..2386
                     /locus_tag="ACG89C_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4901168.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2567..2752)
                     /locus_tag="ACG89C_002678"
     CDS             complement(2567..2752)
                     /locus_tag="ACG89C_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901169.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            2789..3082
                     /locus_tag="ACG89C_002679"
     CDS             2789..3082
                     /locus_tag="ACG89C_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS4901170.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3316)
                     /locus_tag="ACG89C_002680"
                     /pseudo
     CDS             complement(3170..3316)
                     /locus_tag="ACG89C_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1253 a          532 c          714 g         1155 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatc
//
LOCUS       DAZUKR010000185         3563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000185 DAZUKR010000000
VERSION     DAZUKR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.189"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(211..1977)
                     /locus_tag="ACG89C_002681"
     CDS             complement(211..1977)
                     /locus_tag="ACG89C_002681"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4901171.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1937..3563)
                     /locus_tag="ACG89C_002682"
                     /pseudo
     CDS             complement(1937..3563)
                     /locus_tag="ACG89C_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         1182 a          659 c          499 g         1223 t
ORIGIN      
        1 ttcaatcttt tatgtttaac tggtttatcg ccaatttttt aaaactttga ttattcctta
       61 tattataaga atagacatta agtgaactaa ttcaatcata ctgattcttt cgtgtattca
      121 aaattagatg ctgtttaata aaattattct ttttgaaaaa gaattataac tatatcccaa
      181 aaattgtagg ttaatttaaa gcctatttca tcatcgtgat tgagcttctt tttcatagac
      241 gaatttctca gatagtttag ttttctttat gagatttttg tatatttctg accttcgata
      301 caactcatta tgagtacctt gatcaactag ttttccttga tccatcacta caattttatt
      361 taccttttca atagacttca agcgatgcga tataattagt actgtcttat cttgtattaa
      421 acgatttaaa ctttcctgaa tacgtttttc attttccgca tctaaactgg cagtaatttc
      481 atctaaaatt aaaattggcg catttttcaa aaaagcccgt gctatggata ggcgttgtcg
      541 ctctcctcca gaaagtgaac ttccgttttc tccgaccaat gtatgataac cgtcaggtag
      601 tttttcaata aagtcttcgc agttagctaa acgagctgct tttttcactt cctcatctga
      661 cgcttgacta tttccaatac gtatattttc caaaatactt gtattaaaca aagttacctc
      721 ttgaaaaaca atagcgattt tgctaaacaa agaagctgga gatacacgtt taatatcata
      781 cccatcaatt aaaatacagc cttcatcata atcatacagt ctagacacta atttcaagat
      841 actagttttc ccggaaccac tagcacctac taacgcagtc acttctcctt gtttagccgt
      901 aaaagagata tgatctaaaa ttggggtatt gttatcatat gagaaagaga catctcgcaa
      961 ctcaatgtca aaagatttca gaggactatc tgatccctct tggatactgg tttccttcat
     1021 agctctaatt cgctgtattt taggagctaa ataaaatatt tcaagcactg cttctttcat
     1081 agaatcgaag gaatccttta tttttattgc ggctagtaag taaccaacga cgtagagtat
     1141 agtcacttca cccgttaaca gtaagtgaac acctactaga atgactactg ctaaactaac
     1201 atatgagaaa atagaggata aggccatgac tgaaaaagta cttaattcta cctttaaatg
     1261 aatcctttca ctctcttcca tttttttgaa aagtctgtct tgaacttttt tagatagatt
     1321 aaagctatta atctcttgct gcaattctat agtttcttga aattcttccg agttttctct
     1381 caaagtatcg taatatttag tattggcttt cgtttgggat ttctttgata acaagattaa
     1441 gacaaaacta aataacgttg gcaaaataac agctaatccc attttgacat taccaaccaa
     1501 aagcatcact gaaataaaag ggaaaaacag agccataccg ccggctttag gtattgcatg
     1561 actcatcgca tgctcaatac cttcaacgtc agacatgata gtttgagata aatctgataa
     1621 attatgtttt gaaaaatagg ataatggtaa atttgacaaa tcgtctgcaa tttgcactct
     1681 taaatgcgca ctttctttat aagtactatt atataagttt tcatactccc tatctaacaa
     1741 ccaatacatg aaaagcaaag taacaaacga tacggcaaaa taaagccaac ggggctttgt
     1801 attttctaaa actaattcgt ctatcagcat cattataatg aatgctggta ccatattaat
     1861 gcagtataca aaaaaactag aaatagaagc tttagttaaa gcttctgcgc cctgatcact
     1921 taaagcaaat ttgtttttat aaaaactttt cattgtcaag cctccaatca tttgcattct
     1981 cataattagt gaccaattgt ttatacactt gatgtttagc cattaattca ttatgatttc
     2041 cacgttctac tattttccct tctgacaaga caagaatttc atctacagat ttaatgctgg
     2101 ataatcgatg agctatcata ataactgttt tttctttcat cagatgttga aaagcctttt
     2161 gtagttcata ctcattatct ggatcaatag atgcactggc ttcatccata ataacaatct
     2221 ttgcatcttt taaaattgct ctggcaatag ctatacgttg tttttctcct cctgacagat
     2281 aaactccttt actaccaatt aaggtatgct ctctttcggg aaatttttct aaaatcgatt
     2341 cgcaaccggc taatcttagc gcgtcaaata ctttacttgc tggcgcttga ggatttgcta
     2401 tagaaacgtt atcgtaaata ctctgattga atagtttcgt atcttgaaag acaaaagcaa
     2461 ttgtgttaat aattgtgtct tttgaatagt gatttaaagg cttaccacca attttaatct
     2521 gtcccttatc cacattgtag aaacctgaaa ttagtttagc tatcgtactc tttccgcttc
     2581 ctgattttcc tactaatgca taaattcttt tttgttctaa aactaagttg aaattttcta
     2641 aaacacactc ttctttgtat gaaaagctga cattttcaaa tgcaatgtca caatttttaa
     2701 tctgtgtctc gttcccatat tttagttggt cttgcttcat ttctaaataa agggagtcta
     2761 gtgtttcgac tgcatatctt gcttcaaaca agtgcatact tacatacatt attctcatga
     2821 aagaaacaaa aataattcca cttaaaaaaa acaacatcaa caagtcaaca gctaatatct
     2881 tgggagtggt aagagaagtt ataaaaaagg ttataggaac tatcaaaatg caaactatgc
     2941 caaaaaatag ccattgaaaa agaacaaaag gtgttttccc actcaaagaa tacttatatg
     3001 caaacttagc ataatcgtta attgctttat tcaaggcttt aaatgatgtt actttagcac
     3061 caaatatctt tataacttga attccgcgga tatattctac cgtttcagag cttaacacat
     3121 ctaacgattc ttgatatttt tgaataaaat ttgtattctc tgtcattttc tttaacaaga
     3181 atacaccaat tactaacatg gcgattagaa ccaatccaat tttaaagtta atgaaaaagc
     3241 ctaaggcaac tgataaaaca ggtgttacta tggcttgagc actatctgga atcaagtgag
     3301 caattgccat atgtgtttta gccgcgttgt catcaattgt ctttcgaata tatcccgaag
     3361 aatttaaatc aaaaaaacga aaacttgctt ctgttaaacc gctaattccc ttttttctca
     3421 aattagtctc taagcgaaac ccaaggatat gagaaaataa gccagaaaaa atataaaata
     3481 aagctctaaa gttaaccaag caacagtttg aaccgcataa ctcttagcta aatattcatc
     3541 tcctacaata attagagact gaa
//
LOCUS       DAZUKR010000186         3545 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000186 DAZUKR010000000
VERSION     DAZUKR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.190"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="ACG89C_002683"
     CDS             complement(<1..2303)
                     /locus_tag="ACG89C_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4901172.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ACG89C_002684"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ACG89C_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          989 a          789 c          560 g         1207 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gaatccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacac ttttataaaa cgacgaaaaa taaatcacta ccagtcaact
     3541 tctat
//
LOCUS       DAZUKR010000187         3528 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000187 DAZUKR010000000
VERSION     DAZUKR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.191"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1875
                     /locus_tag="ACG89C_002685"
     CDS             109..1875
                     /locus_tag="ACG89C_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS4901173.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1890..2327
                     /locus_tag="ACG89C_002686"
     CDS             1890..2327
                     /locus_tag="ACG89C_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4901174.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2340..3491
                     /locus_tag="ACG89C_002687"
     CDS             2340..3491
                     /locus_tag="ACG89C_002687"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4901175.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1183 a          631 c          766 g          948 t
ORIGIN      
        1 ctgacatact caaacctttg gcacaaagtt tcgtgccaaa gtacgactat ttctcttttt
       61 agtaagcgga tacaattaga gtatcaaaag gagagaggga gagacaaaat ggaacggacg
      121 attcccaaaa acaacatttc gctaaaagcg agggtacaaa aattaggcag tactttatca
      181 agcatggtca tgccaaatat tggagcactg attgcctggg gtgtattaac ggctctattt
      241 atcccagatg gttatttacc aaatgaggct ttggctacca tggtaagccc aatgctaact
      301 tatctgattc ctttattgat cggttatact ggcggaaaag taattgctgg tgaccgcgga
      361 tcagttgttg gggcaattgc aacgatgggt gtgattgtcg gaacagatat tccaatgatg
      421 ctaggcgcga tgatcatggg acctcttggt ggatatgcca tcaagaaatt tgatcagcta
      481 ttccaaaaac ggatcaaatc cggttttgaa atgctagtca ataatttttc agcaggtctt
      541 atcggttttg gattagcgtt attaggtttt tctgcgattg gtccagttgt cgatgcattg
      601 actcaagcaa tggcaaaagg tgtagagatc attttatctg cccatttgat tccattgaca
      661 agtattttta ttgaaccagc aaaaatctta tttttgaata atgcaatcaa tcatgggatt
      721 ttgacaccat tgggaactga acaagtagca gaggctggaa aatctattct attcttgctt
      781 gaagcaaatc ctggtccagg acttggcgta ttacttgctt ttactttgtt tggcaaagga
      841 gcagcaaaat cttcagcacc aggagccatg atcattcact ttttaggcgg gatccatgaa
      901 atctattttc cttacgttat gatgaaaccg ttattattct tatcagttat aggcggagga
      961 atgtcaggaa gtttcatttt ccaattgatg gatgctggac taagagcgcc agcttctcca
     1021 gggtcgatca ttgcgattct agcgatgaca ccgttcaatt cttaccttcc tgttattttg
     1081 ggcgtgattg ttgccgcagc tgtttcgttt ggtatttctg cagtaatttt gaaagcggat
     1141 gcgaaagata caaatgaaga cttcgaaaaa tctgttcaag agacaaaggc agctaagcaa
     1201 gcttccaaag gtgcccaagg tattcctcaa ggagctacat tctctggcgt acaaaaaatc
     1261 atttttgctt gtgatgcagg aatgggatca agtgcgatgg gcgcttcgat cttgagaaaa
     1321 aaagtacagg aagcagactt gcctcaaacg gtgaccaatc aagcaatcag ccaactgaca
     1381 gatgactcga atacattgat cgttactcag gtcgaactac aagaaagagc aaaacaaaaa
     1441 gcaccgagcg cgcgttttgt ctcagtagaa aactttttga attcacctcg atatgatgaa
     1501 atcattcaag aattaagcag caagcagaaa acatcagttt ctgaaattga gaatgagcca
     1561 gcagaagagt ttctttcagc aaacatcaaa caaatcaaag agattctact tgtccacgat
     1621 gatcgttccg gatcagcaac aatggggatg accgtgctca aagatatatt agacaagaac
     1681 catttaacta tcccgttaag aaaagtcaat atccaagatt taaaagatga acctgattca
     1741 ctgatcgtag ccaaagaaag tttagcggaa aaagtacaga tggctgcacc tcaatccatt
     1801 catttatcag tcaacagcat ggtcaatccg caaaaatacg aatcgattgc acaatatttg
     1861 aaacagacag cttaagggag gaaaacagca tgcaaagttt gccattcgac atgatcgagc
     1921 tcaatcgatc ttttaaaacc aaagaagaag cgatccgcta ttgcggtcag atgcttgtag
     1981 aagcagggtg cgttgatgag gaatatatcg atgccatggt agatagagac aaaatgttat
     2041 ctgtttatat ggggaatttt atcgcgattc ctcatggaac agatgaagca aaaaaatatg
     2101 tgaaaaaatc cggcatctgt gtcgttcaag taccagatgg tgtagatttt ggtaccgaag
     2161 aagaagaaaa gattgcgact gttttgttcg gtattgcagg agtcggagat gagcatttgc
     2221 aacttgtaca gcaaatcgca ctttattgca gtgatatgga caatgttgtc cagctagcag
     2281 acgcgctcag caaagaagaa gtaacggaaa atctagcaat tgcttaagga gagagaaaaa
     2341 tgagagcagt acattttggt gcaggaaata tcggtcgagg atttattgga gaggttttgg
     2401 cagctaatgg atttgagatt actttcgtag atgtgaatca cacgatcatt gatgctttaa
     2461 atgaaagaaa agaatacgaa atcgaattag cagatgaatc aaaagaaaaa atcactgtaa
     2521 aaaatgtcaa aggaatcaac aatcaaacaa atccagaaaa agtaatccaa gaaatcgctg
     2581 aagcagattt agttactacc gccattggcc cgaatatctt accttttatt gctgagttga
     2641 ttgcaaaagg tatccaagaa agagaaaagg aaggaagcac aacatcttta gatatcattg
     2701 cctgtgaaaa tatgattggc ggttcagcat ttctagaaaa agaagtgtat aaatatcttc
     2761 cggaaacatc atttactgac aaatatatcg gctttccaaa tgcagcagtc gatcgaatcg
     2821 tcccattaca acaccatgaa gatcctttat tcgtccaagt ggaacctttc aaagaatggg
     2881 tcattgacga tagccaaaga aaaaataaaa aaatccaatt aaaaggggtt ttatatgtag
     2941 atgatctaga accatatatc gaacgcaaac tatttagtgt caatactggt catgcgactg
     3001 tcgcttatac aggtgccttg ttaggttacc agaccattga tgaagcaatg caagatgcgt
     3061 tagttgtggc acaattaaaa tcagttttgc aggaaacagg aagtttgctg attgccaaat
     3121 gggggtttga tgcagagcag catcatgcgt atattgaaaa aatcattcat cgcttccaaa
     3181 acaaaaatat ctctgatgcg atcacacgtg tcgctcggac accattacgt aagttaggtt
     3241 atcaagaaag atttacacgt ccagtgagag aactacaaga acataacttg acttgcccac
     3301 atttgacggc aacaatgggg attattttta attactatga tcctgaagat gaacaaagcc
     3361 gtcaattgca tgaaatgaag atccatgaaa acttggaaca attgattcaa gaagtcacag
     3421 gaatcaatga tccgaaaaca atcgggaata tcaagcaaaa cgtgaaccgt tatgccaagc
     3481 aagtcgctta aaataggata cgaaaaaggg gaatgtgacg aaattttt
//
LOCUS       DAZUKR010000188         3403 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000188 DAZUKR010000000
VERSION     DAZUKR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.192"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..165)
                     /locus_tag="ACG89C_002688"
     tRNA            complement(93..165)
                     /locus_tag="ACG89C_002688"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Gln,seq:ctg)
     gene            complement(211..639)
                     /locus_tag="ACG89C_002689"
     CDS             complement(211..639)
                     /locus_tag="ACG89C_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901176.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(699..1457)
                     /locus_tag="ACG89C_002690"
     CDS             complement(699..1457)
                     /locus_tag="ACG89C_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4901177.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            1752..1949
                     /locus_tag="ACG89C_002691"
     CDS             1752..1949
                     /locus_tag="ACG89C_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901178.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2055..2537
                     /locus_tag="ACG89C_002692"
     CDS             2055..2537
                     /locus_tag="ACG89C_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901179.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(2595..3191)
                     /locus_tag="ACG89C_002693"
     CDS             complement(2595..3191)
                     /locus_tag="ACG89C_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4901180.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         1032 a          562 c          655 g         1154 t
ORIGIN      
        1 actaaataca agtagtaaaa ataatacaat aatccccctg caaaaagatt gttcttttac
       61 agggggttca ttttttcagc aagcacatca aattgggatg gcagggttcg aacctacaat
      121 tgcttgattc agagtcaagt gccttaccag tttggctaca tcccatttat tcaacaaaat
      181 aatcatagca ctttcctaaa aaaacgcaag ttatttttct tgatcggata gttgttgctt
      241 tactcgtaaa aggcggcgta ctagtttctt ttctaatcca gcaggaaggg caatcagttc
      301 tgctactgac aagacggtgt ccaataactg atattctttg acaggttcat tcataatatg
      361 atagatgttt gctaaacgtt caatatattc aaagcgagta tactctgttc gagcaattag
      421 aaatttcaat aaatcggtgg ctttctcgta ctcttgatct gcgtaataaa caagagattg
      481 acgatacaat ttgatttcca gttcattgag ggtagcaggt ctgcgacgct cgttccactc
      541 attaatagag actttacctt gaacatttcc cttgcagtca cggacaaaaa taatcggaat
      601 ttcatttgga tccatttttt tatgtgtgaa tttttccatt atctacgctc ccttcaaagg
      661 tttggtatct tgagtataag ggatttttgt ttgaattact agcttttctc caaagttagc
      721 atctgtccac ccattgcagt gaaaatcgcc gaaaaatcgt tgatcggtac tgctcgattt
      781 tttacaccat agggatcgtt gacataaata ttttctttgt catatccagt gactacacaa
      841 gcatgaacaa gaggagtgac tcttacttct ccattatttg tggtccaagt cataaaatca
      901 gtttcatctg ggacttcaaa attgatagtc acagaagtcc agacaggatt tcctttagat
      961 agtgcttttt tgatatcatc gagttgagca tcggtttttg gaaaagtgtg ataatccgtt
     1021 tggacgattt gctgagcaag ggaggcaact gggggaacat agacgaccat agcccagtct
     1081 ccgccactga tatcaccaac aaatccttca ttaggatcgc catgagtccc atctgcattg
     1141 aaaacaggga cgtaatccaa ttgctcagct aactggtttt tagttgtttc ataaccataa
     1201 aaagataaaa gcatagaaag cgaagtgact tcacagccat tttctaaaga aggagaatca
     1261 aactggctct ctaatggaac atctagtaaa acttctgctt tctcttttgt ctttttcgtt
     1321 ttttgtgttg aacttttttg tgcagtgttc attgtatgtt ctggagaatg gatatgtagg
     1381 taataacttg taccgcttaa taaaagagca gctgtgcccc ctagaaacaa tagttttttg
     1441 aacgtttttt tcttcatttg aaatctccct tttcagctgt ttatctttcc tattataaat
     1501 gtatttttat tggaaatgga agtattatat tttaatatgt aggaaataat tagtttttaa
     1561 ggaatatgtt ttaatttttt gaaaaaaatt cttctaaata caacagctgt tttaattgtg
     1621 aaaacgaaaa cagtacgcta taatgcctgt aaggttgatt tttaactgta aataaaaatc
     1681 aatgagttaa atctccaaag aaatagcagt ggtaatgtgg agaaaagttc cagcagttta
     1741 ggaggatttt gatgacggaa gaaatgatta atttaggaga acaatatgca tgcaaaccaa
     1801 ttggttttac aaaaacggtc attggtgaag ttgtatcaaa aatgacaaac tgcgccgtgg
     1861 tcaaagttgc ccaatgtgct gcagaggacc aagaattatt agatgaaaaa gcaagcatgg
     1921 ttgttgcaaa atatgataca tttgaataaa gaaaacaatt ggacgtgcgg cgtgctgcaa
     1981 gtccaatttc tttgagaagg taaatcagta ttcttttcct attttctgga gaagaaaaag
     2041 cgaggcgagt tgtcatggta acattcttta cggttctatt gtttttattc gttgttgtga
     2101 tgtttttatt tctttggctg gttcgaaaag aaattattta tcggactgtg cgaaatcgat
     2161 gggtctactt ggtgataccc ttcttagtgt cactagtcat ttggtataca ctcatcagtc
     2221 atcctacagc agatgaattg gcaaaaggga tcttgtcagc aatgattttt attagttttt
     2281 tgttggacag tagaggaatc acagaagaag gacttgtttt gaatagcttt gacaaaaaag
     2341 gtgttccttt ttctgagatc aataaaattg tattatatca gccaaaaggt tctaaaatcg
     2401 taaaaatgaa ttttttccga aacggatggc gaggaccgat gctgaaattt tctgcatcat
     2461 tagaagaatt agttccattt ttgtcccaac gcttaaacga tgaggcagag attgatatta
     2521 tgattgatcc agaataaaaa cagaggttgc gacaagatgt ttgtcacaac ctctgttttt
     2581 tatttcaaat aaaattatcc aaatacgcgg aagcgatcgg catcgttcat gtattctttt
     2641 tccccagccg gtgtttctgg tgaaccgaat actaattgcg aacgtagttt ccagtttgaa
     2701 gggatattcc attcttttgc aactgcttcg tcaataactg ggttgtaatg ttgcaagttt
     2761 gcgcccaatc ctttatcaac taatgctacc caagtattag ctgttgcgat tccgtttgat
     2821 tgttctgacc aatctgggaa gttgtctgca tataattcaa attgttcttg tagacctttt
     2881 accacgtcag tatctttgaa gaataatact gtaccataac catttttgaa accagctaat
     2941 ttgttttgtg tatttgggaa agcttctgct ggagtcaatg ggcgaagtgc ttcttcagtg
     3001 atttcccaaa gtttttcatg tgcatctccg aaaaggataa ctgcgcgtgt tgattgagca
     3061 ttgaaagcag ttgggctttc tttaattgct tctttgatca aagttgttaa ttcttcatta
     3121 gaaagtgaaa cattacgtcc tagatgatag attgaacggc ggtttttcaa agtgtcagta
     3181 aaagttgtca taataaaatt tctcctttga tttgcgtttt gcattttttt aattgattac
     3241 gaaatgaagt atagcacctt acaaaaagta agtaaacaga tatgctcaca aaaaataagc
     3301 gaagttcaaa aaacaatttt aataagaaaa atcgttatgt aaggattttc ttagttatcg
     3361 ctttcattgt tgtgttttag gaatttttgc ttatttgaaa aaa
//
LOCUS       DAZUKR010000189         3335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000189 DAZUKR010000000
VERSION     DAZUKR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.193"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            292..>3335
                     /locus_tag="ACG89C_002694"
     CDS             292..>3335
                     /locus_tag="ACG89C_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4901181.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         1244 a          516 c          686 g          889 t
ORIGIN      
        1 taagaaaggg taatattttg aaaacatgct tcatcgcata gaaatgaact taatttcaga
       61 aagaatacat acatatcttt ttatatgttt tcagctgctg aatggttaaa aagcacaaat
      121 atccgaaaaa aacatgaaga aatgtacttt ttttgttgta atgttcatga aattctctaa
      181 gtaaattagt taacataagg ttagtaagat tcctattctg tgaataatac gtatcagatt
      241 tatcttttat tcttactagg aaagtaatta ttaagggagt tgatttgaat aatggttagc
      301 aagaataata agagagtatt tctagaaaaa acaaagaaac gagtattgaa gtacagtatc
      361 aaaaaattaa gtgttggggt tgcatctgtt ttgattggtg ttggtcttgt cttgggaaca
      421 actgaactag ttaaagcaca agatgaaatt tctccaagca gcccacttga aacagcgata
      481 agctccgtac aagtaggtga caaagtagct tcggggaata cttttcagga gaatcctggt
      541 tatacgaaaa attataactt ttcggattta caatttagtc ctcaagaact aactggagac
      601 acactaaaag gcaatacaat tggatttgaa gtttacggaa aacataatat tgcagcatca
      661 actaaaaact gggaaatccg tcttcaatta gatgaacgat tagctaaata tgtagaaaaa
      721 attcaggtcg atccaaagaa aggaattgga tcttctagac ggacctttgt aagaattaat
      781 gattcgctgg gtagacctac aaatatttgg aaggtcaatt atattcgtgc aagtgatgga
      841 ttatttgctg gggcagaaac aaccgatacg caaaccgctc ctaatggggt aattacgttt
      901 gaaaagagcc tagatgaaat ttttaaagag ataggcatag acaatctcaa gaccgatcgt
      961 ttaatgtatc gaatctactt ggtaagtcat caagacgatg ataaaattgt accgggtatt
     1021 gatagtacgg gctacttttt aacagattca gatgatttct ataatagctt agatgtatct
     1081 gaaaataatc ctgaccagtt taaacatgga tcagtaagtg caaaatatga gaaacctaat
     1141 actcaaacaa aagatggttc aggatccaca ggtgctaatg gagctataat cttagatcat
     1201 aagttaacga aaaattataa tttctcatat tcatcttccg caaagggcac gccttggtat
     1261 gctaattaca aaattgatga acgattagtt ccatatgtag cgggtataca gatgcacatg
     1321 gttcaggctg acaaagtaac atatgatgtt tcgtttgaat ccggaacaaa agtagctgat
     1381 ttagcaatcg agcgacggga aggtcatgaa aattatggca taggttcaat aactaataat
     1441 gatttaacta aacttatcga ttttgctaat gcaagtccac gtccagtcgt tattcgatat
     1501 gttttacaat tgactaagcc attagatgaa atcttagaag atatgaaagc aacagcacaa
     1561 gttgaagaaa ataaaccatt tggtgaagat ttcatctttg attcttggtt gtcggatacg
     1621 aataaaaaat taatccagaa cacttatgga acaggttatt attatttgca agatattgat
     1681 ggtgatggaa accctgacga taaagaagag agcggagaca cgaatccata tatcgggaaa
     1741 cctgaattag aagaagtata tgatgttgac acaacagtta aggggaaagt attcatccac
     1801 gagttagcgg gaacaggtca caaagcccaa cttgttgata aagaaggtac tgtattagca
     1861 gaaaaaacta tcgctccaaa tgaaaaagat ggggctccaa tttcagatac tgtagaattt
     1921 gaatttacgg gtgtagattc aagtaaatta atcgcgaaag atgaattaaa aatccaaatc
     1981 gtttctccag gttttgataa accagaagaa ggttcaaccg ttattaagga atcaccaaaa
     2041 gcggttgata aacaaaccgt ggtagttgga tttaaaccag atgctaaaga atcaattcgg
     2101 aataataaaa acttacctga agatgcagag tattcatgga aaacagagcc tgatacttct
     2161 aacgttactg atagtacgaa aggtattgta actgttaaga tcggaaatcg aactttcgac
     2221 gtggatgtag agtttgctgt aaaagcttct caagctatgg aaaatgatgc aacatacgta
     2281 cctataacaa caactccaga aacgacagtt caaagtggaa aacctacatt tgataaacca
     2341 gatgttcctt tagctaaaga tgccttttca atttcagatg tctataataa gtacttcggt
     2401 aatgcaagtg ttgatgcaaa cactggtatt gttacattca ctccagctaa aggtgtagga
     2461 gaatcggagc cgattactgg aacaattcct attaaaattg tttaccaaga tggttctgta
     2521 ggcacgaccg atttagcagt aactgtaagt aaagatattt atgaaaatcc aggagaaaac
     2581 attcctgcag gctaccacaa agtaaccttc accgcagggg aaggaacaag tattgaaagt
     2641 ggaacaacag tctttgcagt gaaagacggc gtaagcttgc cagaagataa acttccagtg
     2701 ttgaaagcaa aagatggtta tacagatgcg aaatggccag aagaagcaac tcaaccaatt
     2761 acagcagatg atacagaatt tgtatcaagt gcaacaaaac tggatgatat cattgaaaat
     2821 ccaggagaaa acattcctgc aggctaccac aaagtaacct tcaccgcagg agaaggaaca
     2881 agtattgaaa gtggaacaac agtctttgca gtgaaagacg gcgtaagctt accagaagat
     2941 aaacttccag tgttgaaagc aaaagatggt tatacagatg cgaaatggcc agaggaagca
     3001 actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa actggatgat
     3061 aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag tgactacaga attgaataag
     3121 gaaccagacg catttgaggg aattaagaac aaagaagatt taccaaaaga tactaagtat
     3181 acttggaaag aaaaagtaga tgttagcgca gctggaaata aaaaagggac tgttgtagtg
     3241 acatatccag atggatcatc tgatgaagtt gaagtagatg ttacagtgac agacaatcgt
     3301 tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAZUKR010000190         3202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000190 DAZUKR010000000
VERSION     DAZUKR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.195"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(422..1036)
                     /locus_tag="ACG89C_002695"
     CDS             complement(422..1036)
                     /locus_tag="ACG89C_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4901182.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1642..2019)
                     /locus_tag="ACG89C_002696"
     CDS             complement(1642..2019)
                     /locus_tag="ACG89C_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901183.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2319..3200)
                     /gene="galU"
                     /locus_tag="ACG89C_002697"
     CDS             complement(2319..3200)
                     /gene="galU"
                     /locus_tag="ACG89C_002697"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4901184.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          976 a          658 c          496 g         1072 t
ORIGIN      
        1 tattagaaaa aggtatcctg ttggacaatt ttgttatatc aggtaaactt cattgcaata
       61 atgataacaa atgtactata attactttgt aaagagtttt ggggaagact ctttcatcaa
      121 cgtgtattaa gagacaagaa agaatccata tggattcttt cttgtctctt tttccactac
      181 tttttatttt tctgacacta catattctta taactcattt ctcataactg tgaaacaact
      241 tataaaaata acaaacttta aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa
      301 taactaaaac aaaaaaagta gtcgttgtta atcctcatta ataatcgttt tctttttttc
      361 aaacgctcca tttttatgtg gaacattatt tttttaagga actttgtcaa catgtggata
      421 actattttcc acttgtgtcc tggactagct gttttgctgt aaaatacaaa acttgctgca
      481 gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc tggatctaga tttggcaaag
      541 tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac caaatcagca aattcaaata
      601 atcgcaaacc actgctatgt tgcgatttga tacttcgtga caaatcaaat ccagtaacaa
      661 tgattaatgg atgaccattt tctttgatcc actgagctag ttcgatgatt gccgggtttc
      721 ttccttcatt cgaaagaaga agaaaaatgt cttctgattt gacagaaagc tgatcaataa
      781 ttgcctgtcc tgcaccttct agcgtttcat agacacctcc ggctggattt tcaatcaata
      841 tacttggtac aagatccggg gtttgatccc aaaattcaaa tgctacacca cttagtgtgc
      901 ggctagcata tatctgcaat ctaccacctt gattaatttg tttcgcgatc caagtgccag
      961 cttgaagcat cagcggctct tctatcctta atttctcttc caattggtga taatagtcaa
     1021 aataacataa actcatttat ctctcccgcc tagctatact tgacatgaat agaaacagct
     1081 gcctaatcta aggaagtaca taaaaaaaca gtagttagac agtaacacat gtggtctttg
     1141 ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca ataccttgac atagcaaata
     1201 aaacaacgtg cagaccatgg aaaatatcta cataggggta gaattttcac cctagattag
     1261 gtacaataaa ttaggtcgat gcactgcatt gactatggca aaatgatcgg tcagggtgta
     1321 tgtgtgtgac tggtcattcc ctgattctta gcgccaacta agggtcaggg agcctttttt
     1381 tattcagctg tttctatctg aatttattgt aagtaatctc ttatccaagt gcaagcaaga
     1441 aaatacaaaa aaacattggt atgatttata tatatataaa taacataaaa aaaggaatgg
     1501 cttatcccga tacaacaagc attcctttaa aaaagattct tttatataag tgaaaaaagc
     1561 ttttttccac caattaaata aatctctcat ctcaatagtt tatccattat tttatctttt
     1621 tttgcgtata cctactaaat attaccgtct tcttctcgtt cttctacttt gttcgagaga
     1681 atctgtacta gaaaaagtag ttgtatgtct accttttctg cctatccgag actttctttt
     1741 catatatggg atataaatga ttagatacag tacagcaaaa aataaagcaa atacgcaagc
     1801 ttcccaacta cctaacgcca attcaaccat caaggtagca accccaacgg atgctgcata
     1861 ccagataaaa atcagaaacg tttctttttt tctgaccatg cgaaacatga taaatagact
     1921 aataagcaat agtggcaaca caaatatcca attgcttcct tccatcagcc acttgagtat
     1981 atcaagcagc gactgtgcaa cagcgactaa ttttcccata gtgaacacac accttttttt
     2041 ataattaaaa aattcgcctt tttattctac tatgaatctt ctggcttggc tttcatttct
     2101 cttatgtaat cattttcttt tatacaactt aaaaaaaaat taaaccatta gcataatatt
     2161 ttctgaatct attcacgaaa gtctttttct atctttcgtt ttccacgact caactgatct
     2221 agagtcaaaa aactaaaacg acagttgcgc aaatgacgca caagctgccg ctttaataac
     2281 aaatagcctt tcattctcag caggccactt atttccagtt aactttctgt tcttagcttt
     2341 ttactcaact ggataatata tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca
     2401 tattcaatat ttgtcttcat atatcctaac ttatcgccta cgtcgtatct ttttccttta
     2461 aactctctcg catagaccgc ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt
     2521 atctcgtttc ctgctcctgg ttcttgagtt tctaatatct caaatatttt aggagtcagt
     2581 agataacgtc cgataatggc taaattactt ggtgcttttc ccatttcagg tttttctaca
     2641 aaccctttta ctctgcaacg acccttttcg ctatctgttt taggatcaat aattccgtat
     2701 ttagatgttt cattttctgg tacacgcatg acggcaacag cggaagcttg tgtttcttca
     2761 taatccatca tcaattgttg agtcaatggg atatcatctt tcatcaaatc atcccccagc
     2821 atgacgacaa acggttcatt tcctataaat gcttttgctt gaagcacggc atcccccagt
     2881 cctcttggat gagattgacg gatgaagtga agattgactt gagtcgtttc ttcaactaaa
     2941 gcgagtaatt ctgttttttc cttttcccgc aaattgtttt ccaattcgat atttgcatca
     3001 aagtgatctt caatgggtct tttttctttt cccgttacga tcaagatgtc ttcgattccc
     3061 gaatccaatg cttcttccac aataaactgg atcgttggtt tatccacgat tggaagcatt
     3121 tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg
     3181 actgcttttt tgaccttcac tt
//
LOCUS       DAZUKR010000191         3180 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000191 DAZUKR010000000
VERSION     DAZUKR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.196"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG89C_002698"
     CDS             278..787
                     /locus_tag="ACG89C_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4901185.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG89C_002699"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG89C_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4901186.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..>3180
                     /locus_tag="ACG89C_002700"
     CDS             2322..>3180
                     /locus_tag="ACG89C_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4901187.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTI"
BASE COUNT         1122 a          513 c          632 g          913 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt cctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
//
LOCUS       DAZUKR010000192         3132 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000192 DAZUKR010000000
VERSION     DAZUKR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.197"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG89C_002701"
     CDS             <1..1152
                     /locus_tag="ACG89C_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFS4901188.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG89C_002702"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG89C_002702"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4901189.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG89C_002703"
     CDS             2728..3003
                     /locus_tag="ACG89C_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS4901190.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..>3132
                     /locus_tag="ACG89C_002704"
     CDS             3009..>3132
                     /locus_tag="ACG89C_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4901191.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNV"
BASE COUNT         1066 a          566 c          729 g          771 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cg
//
LOCUS       DAZUKR010000193         3128 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000193 DAZUKR010000000
VERSION     DAZUKR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.198"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACG89C_002705"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACG89C_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ACG89C_002706"
     CDS             1360..1992
                     /locus_tag="ACG89C_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4901192.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2200..2424
                     /locus_tag="ACG89C_002707"
     CDS             2200..2424
                     /locus_tag="ACG89C_002707"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS4901193.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2526..2759
                     /gene="relB"
                     /locus_tag="ACG89C_002708"
     CDS             2526..2759
                     /gene="relB"
                     /locus_tag="ACG89C_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4901194.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ACG89C_002709"
     CDS             2749..3018
                     /locus_tag="ACG89C_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4901195.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAZUKR010000194         3054 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000194 DAZUKR010000000
VERSION     DAZUKR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.199"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..354)
                     /locus_tag="ACG89C_002710"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="ACG89C_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="ACG89C_002711"
     CDS             complement(614..1162)
                     /locus_tag="ACG89C_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901196.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="ACG89C_002712"
     CDS             complement(1163..2020)
                     /locus_tag="ACG89C_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4901197.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="ACG89C_002713"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="ACG89C_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="ACG89C_002714"
     CDS             complement(2582..2911)
                     /locus_tag="ACG89C_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4901198.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..>3054)
                     /locus_tag="ACG89C_002715"
     CDS             complement(2988..>3054)
                     /locus_tag="ACG89C_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4901199.1"
                     /translation="KVDEDEHVVEIYSARTHYESQ"
BASE COUNT          893 a          620 c          384 g         1157 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac ctta
//
LOCUS       DAZUKR010000195         3033 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000195 DAZUKR010000000
VERSION     DAZUKR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.200"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1807
                     /locus_tag="ACG89C_002716"
     CDS             <1..1807
                     /locus_tag="ACG89C_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS4901200.1"
                     /translation="NKAIRKQLFDISTELDSHQFNEFRNSIRLSDYEFYYTSKSNLNF
                     CINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALRDVLREMK
                     NNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQLNSIIG
                     SVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVEFEACR
                     SRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEISRMNV
                     LKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVILVEG
                     DAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTDLDE
                     QAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKENILT
                     YVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFL
                     IKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNI
                     AIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            1804..>3033
                     /locus_tag="ACG89C_002717"
     CDS             1804..>3033
                     /locus_tag="ACG89C_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4901201.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT         1154 a          410 c          504 g          965 t
ORIGIN      
        1 taacaaagct attagaaagc aattatttga tatttccact gaattagact ctcatcaatt
       61 taacgaattt agaaattcta ttcgtctatc cgactatgaa ttttactaca catctaaatc
      121 aaatctaaat ttctgtatca atgaaaatta tgataaattg gtaggaagac tcaatgacaa
      181 tatagctagt aatcctgaag aagacgatga agctctgttg ggctcaagaa ttgacatgtc
      241 agatattttc aagcatattt cggttgttta tgttgacgct cttcgagatg ttcttagaga
      301 aatgaaaaac aatcgaaacc cagtcaaaag aatcatggaa acaattgaat caaaaatatc
      361 atcagataat gttgagtcat taaaaacaat tatacagcaa ttaaatgaga ctattaccag
      421 tgttcctgaa attaggaaaa ttggcgaaaa tattaatcgg caactaaata gcataattgg
      481 ttcagtgtat tctcctaatc ttcttctttc ctcaactatg tcagatgata tgggttcttt
      541 agcaaaattt ctttcaatga agccagaaca aaatatcgac ttagacttac tagggttagg
      601 acatctaaat atgatttatt tagctttgaa aatagtcgaa tttgaagcgt gtcgttcaag
      661 agaactactt aatattatgt tgattgaaga accagaagct catattcatc atcatattca
      721 aaaaactctg ttcgaaggat taaatcttca aaaaaactat actcagattc tcatgaccac
      781 acattctgta catttagctg aagtgtctga aatatctcgg atgaatgtat taaaatcgtt
      841 taatggcaaa tctatcgtta tgaatccagc aaataatctg aactcatttg ctaaagaaaa
      901 gttaaaaaag aacaatctaa atctaataaa cgcagtagag agatatttag atgttaaaag
      961 aaatggattg cttttttcta aaggtgttat cttagttgaa ggagatgctg aagaaatact
     1021 tattcctcaa atagtaaaaa aattatttgg tataaactta gacgaacttg gaataggttt
     1081 gattaatgtt ggtagtactt cgtttgaata tatcgctagc ttgtttgacg atgatagaat
     1141 acagagaaag tgtgctattg taactgattt agatgaacag gcaatcccat cagaacatcg
     1201 attgtataaa agcactgcac aagagaaagg tcaagcaaga aaagaaaaat tatcctccct
     1261 ttttaatgaa aacaattggg ttaattgttt ttatgctaat acaacatttg aaattgagtt
     1321 tttagatgtg aataaagaaa atattctaac ttatgtcagt ccaattatag atgacgcatt
     1381 caaagataaa aaaacaataa ctgcttataa agaagaaata caaagtgaac aatatttaga
     1441 aagaaattta gcgatgttaa aactagccaa tcatgttggt aaggggtggt ttgcaattga
     1501 gttaggtgaa aaaattgact ttttaatcaa aattcctaag tatattctta atgctattgc
     1561 ttttgcatgt caggaaatag tagatctctc aatttataaa aaaattctac tccatacaat
     1621 cgacttgtac gaggaaacta cagaattgtc agctttatca gctagtttaa aaacaagtga
     1681 tacagctgat ttgaatatag caataatttc tttattagag tactttggta aagatgagga
     1741 tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa atactttatg ctgaggtttc
     1801 aaaatgacaa atatcttcga aaaaatcgta aatgatttaa atttagaaca aaaagatgtt
     1861 gtggaaagtg aagataacct ttatgtaaat gcatgtcctg ggagtgggaa aacaagggta
     1921 cttacaagga aaattgctta ccaagcaatt aaacacgatg gatcactaaa aagaataatt
     1981 gctatcacat acactaatag agctgctgaa gaaattaagg aacgcctaag tttacttagt
     2041 atagatgatg atattaatat ttgggtgggg acaatacatc agttttgtct tgaatttatt
     2101 atttatcctt ttaaaatgaa tctctcaaga gtatcaaaag gatttacaat tattgatgat
     2161 tatactcaaa gagcttacgt cagccaaatt aataatatgc tgaatttaaa tctcaaaacg
     2221 tatgaactga acaaaattga tttgtcactt tctcatgatt ttaaaattaa tgaagttcag
     2281 tatactcaag tttctgaaat gtatcacact atgttggaaa aaaataaaga aattgatttt
     2341 gatatgattt tactagtagc gtataaaatt ttaactgtaa acagtaacgt tgcacgtaat
     2401 attgctagta atataagggc gatttatgtg gatgaatttc aagatactag agaattacaa
     2461 tacaatgtaa tagctaaatt acttcaaaac aaccctcaaa ttcagtcaat gtttgttgga
     2521 gatattgacc aagcaattta tggatcatta gatggtatag ctaaatcagt tgaagaacta
     2581 gaaatgctaa ctagtcacac ttttcaatca aagacgctac atggctgtta caggtctaat
     2641 cagagattag tagacttcta ctctaatttt cagagttgtc cttacgagat tgaaagcaga
     2701 gggaataata aaaacgaccg tggttttata agttatgatt gcaaagttac taaggaggat
     2761 ttacccaaca taataaaaaa tataataaag gaaaagattg aaagcggcat tcatgaaaaa
     2821 gatatttgtg tacttgctcc tcaacactat cctctgtttt cactgggtga gtatttaaag
     2881 aaagaattac cgtattgcaa ttttgatgct ataaatatat caccaattaa ggtaaataat
     2941 catagtttat ttttcaagtt agcaattttg tactttactg aatcaggtga aaaggttcga
     3001 agaagaaagc taattgcaaa cgatatttta att
//
LOCUS       DAZUKR010000196         3012 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000196 DAZUKR010000000
VERSION     DAZUKR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.201"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..359
                     /locus_tag="ACG89C_002718"
     CDS             177..359
                     /locus_tag="ACG89C_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4901202.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="ACG89C_002719"
     CDS             462..746
                     /locus_tag="ACG89C_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901203.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="ACG89C_002720"
     CDS             762..998
                     /locus_tag="ACG89C_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901204.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="ACG89C_002721"
     CDS             1020..1274
                     /locus_tag="ACG89C_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901205.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="ACG89C_002722"
     CDS             1356..1541
                     /locus_tag="ACG89C_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS4901206.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="ACG89C_002723"
     CDS             1608..2003
                     /locus_tag="ACG89C_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4901207.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            2020..2370
                     /locus_tag="ACG89C_002724"
     CDS             2020..2370
                     /locus_tag="ACG89C_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901208.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="ACG89C_002725"
     CDS             2392..2766
                     /locus_tag="ACG89C_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901209.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DAZUKR010000197         2908 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000197 DAZUKR010000000
VERSION     DAZUKR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.202"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACG89C_002726"
     CDS             complement(61..1371)
                     /locus_tag="ACG89C_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS4901210.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACG89C_002727"
     CDS             complement(1383..2801)
                     /locus_tag="ACG89C_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4901211.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          907 a          605 c          453 g          943 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagata
//
LOCUS       DAZUKR010000198         2903 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000198 DAZUKR010000000
VERSION     DAZUKR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.203"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..133)
                     /locus_tag="ACG89C_002728"
     CDS             complement(<1..133)
                     /locus_tag="ACG89C_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HFS4901212.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     "
     gene            203..553
                     /locus_tag="ACG89C_002729"
     CDS             203..553
                     /locus_tag="ACG89C_002729"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4901213.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            967..1785
                     /locus_tag="ACG89C_002730"
     CDS             967..1785
                     /locus_tag="ACG89C_002730"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4901214.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1858..2217)
                     /locus_tag="ACG89C_002731"
     CDS             complement(1858..2217)
                     /locus_tag="ACG89C_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4901215.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          985 a          409 c          519 g          990 t
ORIGIN      
        1 gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc gcaacgatgg
       61 caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt ggtgtaagat
      121 gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta caatttgagt
      181 gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt tttataaagc
      241 aatcattcct gagaacttag atgaggttaa actttattta tgggaagata acggagaaat
      301 tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg atccagattt
      361 tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg aagatataaa
      421 aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa gccatggatt
      481 tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta taacgcattt
      541 agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa aagtaatatt
      601 tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg tgtattacat
      661 tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca aggatgtacc
      721 agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca ctgatttagg
      781 ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa atggcttgta
      841 tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag taagaaaaat
      901 aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg cgacaggagt
      961 gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat ggatggaaca
     1021 tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt atcgcgaatg
     1081 aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca attgagatcg
     1141 ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc ttttgttaaa
     1201 gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga ggtggtcctt
     1261 gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa tagtgcctac
     1321 tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta ttaccataga
     1381 ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt tgctccgact
     1441 atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt agccgggcta
     1501 ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga gtgccataaa
     1561 actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca gtgcgttgct
     1621 tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag ttatgaaatg
     1681 gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc aaatgacgaa
     1741 gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact aggtaattca
     1801 tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag aacagattta
     1861 cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac cactttcatt
     1921 ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc cattaataaa
     1981 ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg ttaaatcata
     2041 tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca tacagcatcc
     2101 ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc catcctttaa
     2161 acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat cgctcatttt
     2221 cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca cctattcccc
     2281 cttaactata actcaattct atataaaaaa tattctattc tcaaataatt gaacttttca
     2341 aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga ttaacgctgt
     2401 aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt aaaacgcttc
     2461 cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga ataatagttg
     2521 cccaacttta aaggttattc ttataatatt ttacttcatt ttccaataga aaattcaaag
     2581 gattactggt ttttctaaaa tagtttagtt taaatacata agtatcctcc gacactgtat
     2641 tagttcataa tcatctaaat acaaaatttt caaataaaaa tatttcaagc tctatcgttg
     2701 acgaatgtaa acgatactta taaagtaata gaaattgatt acatttgtaa acttaaaatg
     2761 gttaatttat ctcacataaa actaagaata aaataatgat taaaagtggt tagtttttat
     2821 acgatttagc ctatcggtaa aaatgaatta gagagtgtaa aatattttgt gtaaatgaaa
     2881 aaatccatac aaaaaaggaa gtc
//
LOCUS       DAZUKR010000199         2887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000199 DAZUKR010000000
VERSION     DAZUKR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.204"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            798..1052
                     /locus_tag="ACG89C_002732"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ACG89C_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ACG89C_002733"
     CDS             1122..1634
                     /locus_tag="ACG89C_002733"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4901216.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ACG89C_002734"
     CDS             1631..2668
                     /locus_tag="ACG89C_002734"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4901217.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1042 a          513 c          574 g          758 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctaca
//
LOCUS       DAZUKR010000200         2840 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000200 DAZUKR010000000
VERSION     DAZUKR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.205"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG89C_002735"
     CDS             <1..1412
                     /locus_tag="ACG89C_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4901218.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2840
                     /locus_tag="ACG89C_002736"
     CDS             1546..>2840
                     /locus_tag="ACG89C_002736"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4901219.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQP"
BASE COUNT         1114 a          390 c          559 g          777 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc
//
LOCUS       DAZUKR010000201         2643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000201 DAZUKR010000000
VERSION     DAZUKR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.206"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..252
                     /locus_tag="ACG89C_002737"
                     /pseudo
     CDS             1..252
                     /locus_tag="ACG89C_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            316..>2643
                     /locus_tag="ACG89C_002738"
     CDS             316..>2643
                     /locus_tag="ACG89C_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4901220.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSL"
BASE COUNT          968 a          351 c          486 g          838 t
ORIGIN      
        1 tatttatccg accgtttgtt ggaacaagtg gttcttttag cagatgaatc cttagaggaa
       61 tatgacacag taagcaaaca aacgatgttg tatcgtgaat tactttcaga tgaagaatca
      121 aaagaagatc tgattgcaac aatcgttgat attttggggt atgcgatttc gccagagtat
      181 cttttcaacg tcttagcgga tcaagcaaaa caagcaacgt tccaattgaa tgatctaaac
      241 aaagcctttt tagcaagtca tatttatgga aaaatactgt cggcaaaatt cgaatagtgt
      301 cgaggaggtg ctgtgatgga agaattaaaa gaacgcattg tcaattatgt catagagaac
      361 tctgagatta aaaataaatg ggggaacaat acagcacaga aaatatctga aaaattaaat
      421 gttagtcgta atattgtctc tcaatatttg aatgaattcg taaaagatgg agaatttgga
      481 aaaatcaata ctcgtccagt aatcttcttt tctttaccgg agaaagttcc aaatcaacaa
      541 atttttagct cttcagagga agcggaaaaa tggtatgata gctcttttag ggttggaata
      601 gaaagggtta tcggaagtca aggaagtctg cgaaatgtaa ttgctcaaat taaagcagca
      661 atctactatc cgccaaatgg cttacccatt ttacttcatg gcgagactgg aactggaaaa
      721 agctatttag caaattatgt gtataaggat ttgttggcca ataattatat agaagagaag
      781 gcatctttca tttcagttaa ttgtagtgaa tatgccaata atccagaact tttcttgtcc
      841 aatttttttg ggcatgtaaa aggtgcatat acaggagccg aagatggaag aaagggttta
      901 gcagaattag ctaataatgg agtcttattt ttagatgaaa ttcatgcatt gagcaatgaa
      961 tgtcaggaga aactttttca gtttatggat acgaggaaat ttcatcggat gggtgataat
     1021 gaaaactggt acacatcaag ttgctggatt atatttgcaa catcagaacc acctgaggat
     1081 aaactattat caactttgtt gagaagaata cctgtaaagc taacacttcc aacattagac
     1141 agtcgttctc tttatgaaag aaaacagtta atcaagatat tttttaaatc agaagaagaa
     1201 agactagcaa agaaaatact gatatctcaa caattattag attttttatt aaactatcgc
     1261 tttagtgaaa atgttggcaa tttgaaaaat atcattcaat tgacctgtgc caatgccttt
     1321 tatttcttag atgatttgga agaactttat attcagatgt atcatttacc acaatgggtg
     1381 aatttcaacg gcatgaaggt tatgaaaaga cagaatgaat taaaatatat tcaaatggat
     1441 gatttggaaa aagtagacga agtttttaat atcaacgaac ttatagagta tctttatcaa
     1501 acgtggaaga aagacactga taagcagcag ctttttgaga aatatgatag atattttaga
     1561 caaaagaatg ttgatatcca aaataattca gctttaacat atcccaccta tcaagagatt
     1621 cgatttagac agtatgttaa ggaaatagag agagattttc aaatagaatt tgactattat
     1681 tatcttcaaa taacgtctat ctttttgaat tcttggattt atcaaatcca attaacatcg
     1741 gacgataaat tatattcctt catcaatgat attaatagtt ggtgtagtcg agaagttcaa
     1801 ttagtcaatc gttttattca aataggggat atttatttaa caaaaattca aaaaaatgtt
     1861 ttggttattt tatttaagtt aatgtgtagt aaagaatttc agcaaaaacg tttagcactg
     1921 attttggctc atggagatct aacggcaacc tgtatagcaa gtactgctaa taggatcgtt
     1981 gagagatata tatacgacgc cattgatatg ccactaatga cttcaagtag tgagattgca
     2041 gacaaaatta atagatactt acaagagtgt accggatatg aagaaattat tttacttgtt
     2101 gatatgggtt cactggagaa aatttatcta aatttggaac tagacgctca aaaaaaattc
     2161 ggactactaa acaatgtttc aacaagaatg gctattgatg ttgcaaatca gatattatta
     2221 gatttaccgc ttaaacagat tcttgaacaa gtaaaagaaa atcaaaatgt ggattatatt
     2281 tatgaatcat caaatattaa gaaggatatg attgtctgtt cttgtgcaag cggattagga
     2341 acttcgttta aattaaaaaa gatacttgaa gatagctttc caagggatac tgatcttgtc
     2401 atagaaacgt gtgactatta ctctttagtg aatgaaaact atttatcgga gcttcgtaaa
     2461 gagaacaata ttctttttat tgttggaaca ctaaatccac atctagaaga tgtagaattc
     2521 ttttcaattg aagatatgat tttaggaatg aatataagta aattctccaa tcaattgaat
     2581 agacacttta atgataatga actgaaagtt ttgaacaata atattctgaa gaattttagt
     2641 cta
//
LOCUS       DAZUKR010000202         2546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000202 DAZUKR010000000
VERSION     DAZUKR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.207"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..951
                     /locus_tag="ACG89C_002739"
     CDS             <1..951
                     /locus_tag="ACG89C_002739"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4901221.1"
                     /translation="KKQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISITNFV
                     IVFMYANMVPTLWTTLISIIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQ
                     SLNFSYNKRKVLSNISFSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGI
                     DINMIDNESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMT
                     KLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDY
                     ILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            944..1972
                     /locus_tag="ACG89C_002740"
     CDS             944..1972
                     /locus_tag="ACG89C_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS4901222.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            2028..2246
                     /locus_tag="ACG89C_002741"
     CDS             2028..2246
                     /locus_tag="ACG89C_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901223.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2317..>2546
                     /locus_tag="ACG89C_002742"
     CDS             2317..>2546
                     /locus_tag="ACG89C_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901224.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEK"
BASE COUNT          997 a          286 c          450 g          813 t
ORIGIN      
        1 aaaaaacaaa ataaattact aacactggga agctgtttgg cacaggttat tttccaatta
       61 tggattggag gaatatttct tattgatgtt attttattag ctaaaggaac aataagtatt
      121 actaattttg taatagtatt tatgtacgct aacatggtcc ctactttatg gactacttta
      181 attagtatta tttggaaatg gcaagactat tcagtgtcat taaatagata ttacgaatat
      241 tattcaaata acaacaaagt ggctggcaca aaaaaaataa ataaaataaa ttctattact
      301 ggtcaatcat taaatttttc atataacaaa agaaaggtat taagcaatat aagtttttca
      361 actgaaatga ataaactatt catgatatca ggagaaaatg gaacagggaa aacaacactg
      421 atgaaattat ttactaaaca gttgccggtt agtaataatc aattattcgt caacgggata
      481 gatattaata tgattgataa cgaatcatta agaaggttaa tttcaatcgc aacacaaata
      541 ccatatataa ttaatagctc cgtgaaggag aatattttaa tggggactaa aaactatgat
      601 aaaaaaaaat tcagtaaatt aatagaacta ttgggaataa gtaaatttat gacgaaatta
      661 ggtattactt tggacgacaa tatttatgat gaaaaaatat tattatctgg aggagaaaaa
      721 caaaaaataa gtttagctag ggcgcttatt aaatcagctt cagttattat attagatgaa
      781 ccatttagaa atttagataa tgaaactcgc gataatttga gggattatat actaaataca
      841 aaaaaagaag tttgttatat aattataact catgatcaca ttctagataa ttttttggat
      901 tcaaatgatg aaatattata tttaaataag gagtatgata ataatgaata gcaaaaaact
      961 tcagatgggt gaaattgtta gaggtcctag tcaaatatct attgatgtta catataaatg
     1021 taattttaga tgtaaacatt gttacaatgc gagtggtgga gacaattgtt ttatggataa
     1081 tgagttgact gatgatgaac tgttagaact tacaaaagat atatgtaatt taaagccata
     1141 taatatttgt ttctgtggag gagaaacttt actgagaaaa aaaatagttt tatcttgtgc
     1201 cactttatta aaagaggcag gtattcctaa tattgctatt gttactaatg gatcattagt
     1261 aactcaggat gttgcaaaag aattattcaa agcaggtatt aatcgatttc aagtaagttt
     1321 agatggaaaa aatgctagta gccatgaagc acttagatta tatccaaaca gttttgaaaa
     1381 ggcaattaat gctatcaaat tatttagaga attgtccgaa gaagtggatg ttgctttttg
     1441 tcctactagt tttaattaca atcaattacg agaagtatat caactagtaa atactcttaa
     1501 agtgaagtct ttaagaattc aaccatttat gcttagtggt aggggaatgc aaaataggga
     1561 aatagtgcca actgtttctc aatatagaaa actagtttct gatattaact atattaaaag
     1621 aaatgaagat tataccaaga tagattgggg agatccaatt gatcatatca tccgacacag
     1681 aactttattg aaagattttt cgaattttgt ttctattagg tcagacggag gattaatggc
     1741 atcaccttac ttgccaattc aagttggaaa tattaaatgt cacagtcttt ctaaatattg
     1801 gcgagcagga ctttataaag ttttttcttt agatttagtt caagatatag ctactcaagt
     1861 ttcttcaata caagatatga gtaaagaatt aaaaaaagga catattaaat ggcttaatga
     1921 agatatagaa attgatattc tcgatgaata caaacatgat attttaattt aggtttatca
     1981 ttggaaagtt tagataaaca aagtactttt agaaaggagg gaaaaaaatg atttctgaac
     2041 ctcaaatgaa taatatgtat gaaagcccaa ctataatggg agctggtgaa gctgcgccaa
     2101 gagcttgttc agcaattatg gtgtgtgtgt tattagcatc tgcaggagta ggtatccaag
     2161 caggagcggt gtatacttat gctgtagttg ctacagtagc tgtagctgca gccgcagttt
     2221 ggaaaacagt aggattagtt ggttaggaat taataggaga ctaactgtct cctattgatt
     2281 ccaatgatta agctaaggag gaattttaag aaaataatga aatacaataa tttaaaaccg
     2341 aaattactta atgagaacat agaatatata agagaagaaa attatggata tttaaccata
     2401 gttcccaaaa atggaactga attgaaagat aacgttttca atccaacagt aagatatata
     2461 ttggctaact gtactggaaa aaacagtgta aaaaaaatag cgaatggttt aaaaaagaag
     2521 tttcctagtt tagaatatga aaaatt
//
LOCUS       DAZUKR010000203         2539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000203 DAZUKR010000000
VERSION     DAZUKR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.208"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1482
                     /locus_tag="ACG89C_002743"
     CDS             <1..1482
                     /locus_tag="ACG89C_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901225.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKGKKYTITLEGTKPATQV
                     FRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTSPQVQICQVPSTSLGNC
                     TIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEI
                     TGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDT
                     ATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDSTILTIPEGYRPPISFEQ
                     LVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            1499..1648
                     /locus_tag="ACG89C_002744"
     CDS             1499..1648
                     /locus_tag="ACG89C_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901226.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1645..2091
                     /locus_tag="ACG89C_002745"
     CDS             1645..2091
                     /locus_tag="ACG89C_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901227.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2093..2230
                     /locus_tag="ACG89C_002746"
     CDS             2093..2230
                     /locus_tag="ACG89C_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4901228.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            2268..>2539
                     /locus_tag="ACG89C_002747"
     CDS             2268..>2539
                     /locus_tag="ACG89C_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901229.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          925 a          435 c          520 g          659 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca acagccacac cgtaccagcc taatctactt
      481 gatgcaccgt attatttgag taaggtggct ttgggtgaga atctaattaa accagaatca
      541 caacaaccag ttactaatag taactatctt attaacacct ataacattaa accaatggta
      601 aaaggtaaga agtataccat cacacttgaa ggaactaagc cagcaacaca ggtttttaga
      661 ccatttttca cacgagcaac aggagatgca tgggaggttg gtgacttaca accagtagaa
      721 gggttaactg atgtttggtc tggaacattt acagcagcag ctgacccaca ccctaccagc
      781 ccacaagttc agatttgtca ggtaccaagc acaagtttag gaaactgtac aattaaatgg
      841 ttaaaactag aggaaggcga cacacgaacc ccgaatattg agcaatataa ataccgagga
      901 atcggcatgc gagactcaaa caatccaaaa gattacgtct gggatctagc accagaatat
      961 gtcgaagaca atctggccac agatattaaa atttctgaaa ttactggtaa agcaaacaat
     1021 tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat taatgaagta
     1081 gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa
     1141 ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt aacagttaca
     1201 aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc tatatatttt
     1261 gtggctttaa acggaagaag cagtggtgga aatgattcaa cgatactaac aattccagaa
     1321 ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg ttctactttg
     1381 aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag aaactcaagt
     1441 tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt gaatcgatat
     1501 gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg tgaatgatga
     1561 agaacattta ccggaagaat gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa
     1621 attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg tactggcgga
     1681 gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac agatcagcca
     1741 ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca aatgtttgat
     1801 cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga tttaaacaat
     1861 ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca gatcaatgcc
     1921 aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa agaaaaagcg
     1981 gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac
     2041 atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta gtatgtttag
     2101 ttttagcgat gtgaaaatga tgtatgattg gggctgtttc actaacgaac aagtaatggt
     2161 tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa atcattagca aagaagagag
     2221 cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag
     2281 gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
     2341 taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca
     2401 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
     2461 aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg gtagaacaca
     2521 cgcaacaaat taaaacttt
//
LOCUS       DAZUKR010000204         2533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000204 DAZUKR010000000
VERSION     DAZUKR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.209"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..379)
                     /locus_tag="ACG89C_002748"
     CDS             complement(<1..379)
                     /locus_tag="ACG89C_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS4901230.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            565..1968
                     /locus_tag="ACG89C_002749"
     CDS             565..1968
                     /locus_tag="ACG89C_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFS4901231.1"
                     /translation="MTASDLIRNFIMMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDF
                     FRYYLAAITGEYSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPE
                     GKYSEVLSDFQSMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNG
                     DDTSRVSKAFPSYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNL
                     LNANAYAMRLARWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYT
                     GLVNTIGNLTLVTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISAR
                     SEALINELITMYPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYS
                     KIKDIASDSLDETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDY
                     WKYDNGISINDSLRNKK"
     gene            1989..2273
                     /locus_tag="ACG89C_002750"
     CDS             1989..2273
                     /locus_tag="ACG89C_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFS4901232.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          860 a          380 c          522 g          771 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac ata
//
LOCUS       DAZUKR010000205         2497 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000205 DAZUKR010000000
VERSION     DAZUKR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.210"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..724
                     /locus_tag="ACG89C_002751"
     CDS             98..724
                     /locus_tag="ACG89C_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4901233.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            747..1451
                     /locus_tag="ACG89C_002752"
     CDS             747..1451
                     /locus_tag="ACG89C_002752"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4901234.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            1643..2476
                     /locus_tag="ACG89C_002753"
     CDS             1643..2476
                     /locus_tag="ACG89C_002753"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4901235.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT          740 a          443 c          520 g          794 t
ORIGIN      
        1 ataagtaaag agaaataagt acctgttttt gtttcctagc aatgaatgcg ttatactaat
       61 ggatgaaaga agttttggct atcaaggagg aatgcacatg catatcgaaa gattaaaaac
      121 aggaatgatt gaagaaaact gttatttggt ctacaacgaa gaagcacttt tgatcattga
      181 ccctggagaa gatgcggaac gaatcaaaca agaaatcaaa aaaacaaacc aacagccggt
      241 agcgattttg cttacccata ctcactacga tcatatcggt gcagtggaac ctttacgcgc
      301 gtattatcaa atcccagttt atgtcagccc actagagcaa aaatggctag gtgatccgat
      361 tttgaatctt tctggactcg gtagacatga tgatattgct gatgttattg tctcgcctgc
      421 agaatatgaa tttgaaatga aaacttatac tttaggaaac atgtcttttc gcgtcgtccc
      481 taccccaggg cattctatag gaagcgtcag ctttattttt gatgattttg tcgtttctgg
      541 cgacgccctg tttagaggaa gtatcggtag aaccgatctg tatacaggga atttagaaca
      601 attacttcat agtattcgga cccagctgtt tgtattacct gatgaatttg cagtctatcc
      661 aggacatggc gatgcaacga cgattgaaca ggaaaaaagg acaaatcctt ttttcaatcg
      721 ttaaaacgac ttaggaggag aataaaatga cagaaactac actttatatc gtacgacacg
      781 gaaaaacgat gttcaataca attgaacgag tacaaggctg gtgtgataca cccctcacaa
      841 agcaaggaca agaaggcatt cattatttag gtcgcgggtt aaaaaatgtc gacttttcat
      901 ttgcttattc tagtgacagt ggtcgtgcaa tcgaaactgc ccgtattatt ctaggagaac
      961 attctaaggg aaaagaaatt ccttatttta ttgaaaaaag gattagagaa tggtgtttcg
     1021 ggtctcttga aggtggatat gacatggaaa tgtggggtgt gatccctcgt gtattgaatt
     1081 ttaaaacata tgatgaaatg tttaccacag aagtttcttt tgaacaaatt gctaatgctg
     1141 tatacaactt agataccgct gggtgggcgg aaacttatga aacattgcga gaccgtgtat
     1201 ggacaggatt tgaagatatc gcccatcact gcgaaaaaaa tggtggtgga aatgttttag
     1261 tcgtatctca tggtttgacg atcgcctttt tgcttaatct gatcgatcct aaacagcctg
     1321 ttcgtgctgg gcttttaaat ggcagcgtaa caaaggtcac ttatgcaaac ggtcgatttt
     1381 ctatccacgg catcaatgat acgtcttata tcgataacgg gaaaacacat agtcgcgact
     1441 cgtttctata atggggatat tttaattttt accagagaat cataaaaggc aaagtctgaa
     1501 cgagggcttt gtcttctttt ttctcttcgt gtacttatct gctttatttc tcaagttttt
     1561 cttaatatta aaaaacagat tgggtttctc aaaaaataac ggtatactat aagatgtatc
     1621 ttaattggga aggaaggaat ttatgttttt tgcaaatatt atcaaagcag tgattctcgg
     1681 tattattgaa ggaataacgg aatggctgcc catcagtagt actggtcatt tgatcctagc
     1741 agatgaattc atcaaactgg atgccagttc acaatttatg tcgatgttca atgtcgttat
     1801 ccaattaggg gcgatcttgg ctgtcgtcgt tctttatttt cacaagttaa acccattttc
     1861 tccaagtaaa tcaacggtag aaaaaaaaga tacgtggaca ctttggttta aagtgatcgt
     1921 tgcttgcata ccagccatca tcattggttt gcctttggat gattggttag aagcaaattt
     1981 ccacaagttc ttaccagttg ccattgtttt gatcgtttac ggtattgcct ttattattgt
     2041 cgaaaaacgc aacaaaaaca aaacacctcg ttggacttct ctgaatgact ttacgtacca
     2101 agcagctctt cttgttgggt tattccaagt cttggcactt gttcccggaa catcccgttc
     2161 tggcgcaacg atcctaggat ctatcttgat tggtgcttcg cgttttgtcg ctacagagtt
     2221 ttcatttttc ttagggattc ctgtcatgtt tggcgcaagc ggcttgaaga tcgtcaaata
     2281 tttagctgaa gggaattcat tcaaaatgga agaaactgtt gttcttcttg taggaacatt
     2341 cgtatctttt gtggtttccg tcttagctat caaattctta ctaagctatt tgaagaaaaa
     2401 cgactttacc gcttttggct ggtatcgtat tatccttggt atcattttga ttggttattg
     2461 gtttattgca atgtagaaaa aaccagaaca cttctct
//
LOCUS       DAZUKR010000206         2455 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000206 DAZUKR010000000
VERSION     DAZUKR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.211"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1315)
                     /locus_tag="ACG89C_002754"
     CDS             complement(<1..1315)
                     /locus_tag="ACG89C_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4901236.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCN"
     gene            complement(1326..1994)
                     /locus_tag="ACG89C_002755"
     CDS             complement(1326..1994)
                     /locus_tag="ACG89C_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4901237.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2124..>2455)
                     /locus_tag="ACG89C_002756"
     CDS             complement(2124..>2455)
                     /locus_tag="ACG89C_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4901238.1"
                     /translation="DPFINYIFENKRIFDLILFQSAGTKYQDIVEKITSFVTKKTVCC
                     LDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVP
                     GWIELLS"
BASE COUNT          856 a          416 c          313 g          870 t
ORIGIN      
        1 tattgcatgt tttataaaga aattccggat catgagtagc tataactact gcaccctttt
       61 cttttaagtc tttaataagg tcagcaatcc tttccatatt aatataatcc aacccacttg
      121 taggttcatc aaatagaaat aaattagttt ttgacaatga agctatagaa accaataatc
      181 tttgtttttc tccaccggat aaatcaaacg gtaattgatc tttcatttta taaagttcca
      241 ttttttttaa aattttttct tgctgatctt tactaactat ttcattcccc agggctaact
      301 cgctcctcac tgattctgtg aaaaattggt aatccatttc ttgcattact aaaaacggcg
      361 atgacaaatt ttttttattt tcgattatct ttcctttggt agggcgaata atcttcatga
      421 gtattcttag taaggttgtt ttccccacac cgtttttccc taccaaacca ataacatctc
      481 caactttgac atcaaaattt atgtctttta gaatattttc ataagtaatt tcttcaactt
      541 ttaatacatc tgcacttacc ttttcgatat agggaacttc aatcgaacta ttaaaaatgt
      601 caaatttcct taatgcgtta ttgttttcag tttttaacaa ctgaatagtt gtagttttta
      661 cagttctatt tttaacaaat agaacttgat caattaattc tttcaaatag taaaaacgat
      721 gttcagctat aacaatagta aatcctttat tttttaaatc acaaagtaag tcagcaagtt
      781 tcttagtcgc tttgaagtcc aaatttgaag acggttcatc caatataatt aatttagagt
      841 ttagcgataa actacatcca atagaaattt tctgccgttc accacttgat agcgaaaaaa
      901 tgtttttatc taatatattt gaaagatcta ataattcagc taattcattg attcttttat
      961 ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat
     1021 ttgttgtaaa gaattgtcct ctaggatctt gacttacgta gccaatattt tttgatatac
     1081 tttcttgtgt taaggattgt agcgagttat tttcaaataa aatttcccca accaattttc
     1141 cgtcataaag tcttggaata agtccattca ttaactttaa tagtgtggat ttcccactcc
     1201 cagaagaacc agtaatcaat gtaagactgc ctttttttat tgaaaaatta aggtcaacaa
     1261 gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt gttaaattca aacaattctt
     1321 tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg aaaacaggat gatatagtct
     1381 aataagctca tgtttgtttt atagtaagaa cttcttttca caggagattc tatccccttt
     1441 gttattgcat tagcggtcat ttcttcagca atcatagttg cacgtataag aacgggaact
     1501 aacagaaatt ccaaatattg cagaggctga aaaaaagaca agcgaatgtt tctcattttt
     1561 aaagattctt taatcattct tatttcagaa cgataagtcg gaaagaatct gatcactact
     1621 gtaatactta acattatttt tttcggtaaa tgtatttttt ctaaagagga taataattca
     1681 ttgctagtat agacactaac caacaaccat gagatcatta cgaccggcaa cattcgaaat
     1741 acaatatata aggatgtcgc aaaaaatata gtaaaaaact ctatcttgat agcatcaata
     1801 attttcatta ctaaaaacag aactgaataa atcaagatga aataaataca tgtctttatt
     1861 ttacgaaaca tcaatactaa aactgtagca ataatgaaac accccacttg aaatatattt
     1921 ccctttccta aaaaaataat gaccggaaaa aaaatattta aagtaatcaa ggttaacgga
     1981 ttgatttttg acactctttc acctccaaaa aataagatcg cactgttatc ttaagctgat
     2041 aaaaatgcac cgttattcag ataacagtgc gatcttatca aaaagcataa ctgagaacaa
     2101 ttctcaatgc aagctttttt taattaactc agtaattcaa tccacccagg aacagaaaat
     2161 tgatacaatc ttctggttaa tagcatagtc ttttcttttg aatagctatg cttcaatata
     2221 tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc attaaagact
     2281 aaattttgat tgatcattcc gatagtcttt aacttatcta gacaacagac tgtctttttc
     2341 gtcacaaatg atgtaatttt ttcaacaata tcttgatatt tggttccagc tgattggaat
     2401 aaaatcaaat cgaatatacg tttattctca aaaatataat taataaatgg atcta
//
LOCUS       DAZUKR010000207         2434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000207 DAZUKR010000000
VERSION     DAZUKR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.212"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(269..490)
                     /locus_tag="ACG89C_002757"
     CDS             complement(269..490)
                     /locus_tag="ACG89C_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901239.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(588..833)
                     /locus_tag="ACG89C_002758"
     CDS             complement(588..833)
                     /locus_tag="ACG89C_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901240.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1073..1687
                     /locus_tag="ACG89C_002759"
                     /pseudo
     CDS             1073..1687
                     /locus_tag="ACG89C_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2057..>2434)
                     /locus_tag="ACG89C_002760"
     CDS             complement(2057..>2434)
                     /locus_tag="ACG89C_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS4901241.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          825 a          424 c          336 g          849 t
ORIGIN      
        1 gaaaactttg caacagaacc gtttttacta tatacttctt cccagaactt gtccgtctcc
       61 ttcaaattat ctctacctct cgagttcatt gggtggtttg cccatttatt aaacatggaa
      121 tcacctttct catactcata tatttatgtc ctatttcaat tatatttata tatgcttaaa
      181 taaatttgta tcctgaaatt tattgtaata tgaagctgta ttttacatat agtaaccaaa
      241 gagttaataa aataaaaaga gcgaaccatt atagatcagc tcttttttca ttgctgtttt
      301 taatcttatc tagaatcact tcaaatgttt caaaggtatt tacatctaac tgacataacc
      361 aaagcaagta cttcttttct ttgtaagtcc aatcaatatt acggtaagag gacattagat
      421 tatggtacct tgctctttgt tcaactgaag ccaattccca gcgtttttca aatatttttt
      481 cttcttccat tattgatccc tcgcaagacg ctacctcaaa cttaaataat gggcagcgaa
      541 agtctaattc cttagatcta agtatagcaa acaaatatga aaaaaactta gaaggtagta
      601 aaagttcttc cgtcttttgc tttgcttcta ttcttaatgt gtttcgttat cggtgcccta
      661 caaagatttt cattaattac acactcattt attgttgtgt attttctgac atttctctct
      721 tgatcaatta ttttaattcc atgggcatcc tgatagttca taggagagga gcggctatca
      781 gaaatagttg cacctatctc ttgaacttct tttacaactt ctggaaaatc catccatcct
      841 gcttcagctg tcaaaagtaa taatctataa tatttatctt tccttttcaa tctaagaaat
      901 cgtcacccca ttctttttct tctattataa actatttaac ttttagaatt caaaaaccgt
      961 ttgtctattt agagtttacc ctaaatagac aaaaatataa cattgaaaaa tttagagttc
     1021 taacgtatga ttagggtgta ttataaacgt tatagaaaag tagggaataa atttgaaaat
     1081 tgcttatatt agagtttcat ccatcgatca aaatgaacaa cgtcaaattg aagaaatgaa
     1141 aaagtttggc gctgaaaaaa tttttatcga aaaacaatct ggagcgacga ttagtcatcg
     1201 gccgattttc caggaagctt tagattttgt cagagagcaa gatattttta ttgtagaagc
     1261 cattgatcga cttggacgca attatgacga gattatcgat tcagtgaatt atctaaagaa
     1321 gaagaatgtg cgacttatta tcaccagttt accgatcatg gctgaagcaa ttggaaatcc
     1381 tctcctagat aaattcatca aagacttaat catttaaatc tcagccatga ttgctgaaca
     1441 agaacgtact gaatcgaaac gacgccaggc acaaggaatt aaaatcgcta aagcaaacgg
     1501 cgtctataaa ggtcgtccta agttatacag tgcagacacc aaagatcctc aacgtcgcct
     1561 tgtctatagg agtatcgttc aggatcttga gaatggggtt gctatctcaa agattgcaac
     1621 agactacaat gtgactaggc aaacaattta ccgaataaaa aaagaaatcg accaactaat
     1681 tgtatgattt cttttttgaa tcacgtaatt attaattagc tttgtcatca atacagacaa
     1741 tcattttaga ttcatctagc taagttgatt acatttatgt taaaaagaat ttagttaaca
     1801 taaatataat tgatgcataa atcgtcctta tttaaaggca tttaacattg acaacttagt
     1861 ataatgtatc ttatgtaaac taataagatt tgaattttat tttatacaca ctcgataata
     1921 gatcaatttt atggtccccc gcatttacga ttgcacaaga gaaacataaa gtcgacctat
     1981 tagcatttct tttcaattca aacattaaaa aagtttatac ctctgctact aaaaaatgaa
     2041 attatctatt taaaatttaa aacggtaaat ctgattcatc caaattggaa aatggatcag
     2101 cctgtattgg agctttgtat ttctccataa taacagatat ctttttttca ttttttaaaa
     2161 aatacctttt aaatttctta tagtcctctg aaatcaaaat atagcgtgat ggatactcat
     2221 caaaagaaga atcgtattct tcctttaaaa ttcccgattt acttaatttt tctagtacat
     2281 caaatactct atcactcaag taactcctta aattgttatc actttcccat tgaactactt
     2341 ttctataagt tgaattatca gttgaaaaga tagaattttc atccataaac gcatatatta
     2401 gcagcaatct ttcatcattg tcaaattcta ttgg
//
LOCUS       DAZUKR010000208         2431 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000208 DAZUKR010000000
VERSION     DAZUKR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.213"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..2281
                     /locus_tag="ACG89C_002761"
     CDS             128..2281
                     /locus_tag="ACG89C_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS4901242.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          832 a          426 c          537 g          636 t
ORIGIN      
        1 tgaaacaaaa agttgatttt atcttgaaat taggtaaaga atttcttgct ccgcccattt
       61 tttctttttt attaagaagt ttttgggtac aatgaagcat gaatgtaaat aggcggaagg
      121 ataggaaatg atgaaagatt ttatgaaaaa aatattaaat acttcttggt tagaaaaaat
      181 aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg ttccttttcg
      241 cttaaatttt ctttttttca ttatatttac gttatttgtt gcgttgattg ctagattggg
      301 ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta cttctacgat
      361 tacggtccaa gaaagtactc ctcgcggaat gatctacgac tccacaggaa aagctttagt
      421 tacgaataaa gcgaatcagg ccatcacata tacgagagga acacaagtta cggctaaaga
      481 tttgcttgaa atcgcaacga agctgaactc gctgatcgat gttccggtag atgaaaatct
      541 gacagataga gataaaaaag atttttggct ggctgatccc aaacatttga aagaagccac
      601 aaaaagactt tcggcaaaag aaaagcaatt agggaacagc gaacaatata gtgcgatggt
      661 caataaagtc acagctgatg agattaattt aaacgaggag caattaaaaa ttgctaccat
      721 ctttaaacgg atgaatagtg catatgaatt aaacactgtc tatatcaaaa actctggtgt
      781 tactgatcaa gagttagctg ttgtggcaga acgcgcttct gaacttccag gagtttcgac
      841 tggcacagac tggacacgag aatataatgc agcaagttca ctgaaaagta ttcttggcag
      901 tgtgacgact gaaaagcaag gacttccagc agatgaagca gagaaatacc tagcaaaagg
      961 ttattctcgg aatgaccgtg taggacaaag ttatttggaa aaacaatatg aagatgtcct
     1021 gcaaggaacc aaaacccaat atgaagtttc ccttgataat gaaggaaatg tttcgaatca
     1081 aaaagaaatc ttctctgggg aaaaaggttc taatttaatg ttatctatga atgcagaatt
     1141 ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa acattgatca acaacggcaa
     1201 agcacagtat tcgcctggtg cttatgcagt ggctatgaat cctcagacag gagaagtgct
     1261 agcaatgact ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga atgctttagg
     1321 cacgattact agcgcctttg atccagggtc tgtagtcaag gctggtactt tgacagcagg
     1381 ctgggcaagt aacgcgatca gcggaaatca agttttgatt gatgagccga ttcgtctgca
     1441 aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc ttgatgcagt
     1501 caaagcatcg gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa agatgttagg
     1561 actagactat acacctggaa tgggactacc tagtttagat gaagaagcta aagcttatca
     1621 gcaattgaga gactctttca aagagtttgg tcttggtaca acgactggga tcgatttgcc
     1681 gaatgaatct cctgggatct cacgttctgt tgattatatg aagaagttca atgcggataa
     1741 tggcaaagaa tggtatgctc caggtaactt taccgatttg gcatttggac agtttgacac
     1801 atatacacca atccaattag ctcaatatgc ttcaacagtc gctaatggcg ggaaacgagt
     1861 acagccgcac ctagtcaaag cgatctatgg caatgacgaa aatggaaact tgggtgaagt
     1921 gaagaaagaa attggcacaa cagtagaaaa tacggtcaat atttctgcag aaaatatggc
     1981 gatcctccgt gaaggattcc accaagttgt ccatggtacc gatccttaca caacagccaa
     2041 accattagcc agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag aaacggttgc
     2101 ggaaggtcat ccagatattg cgacagtcaa tagtaatatc gtggcatacg gaccaacaga
     2161 caatccggaa attgccatca gtgttgtctt gccgaactta ttagacgaac aagaccacat
     2221 aaacttgacg attgctaaag aaataatgga cgcgtattat gatatgttca tggcaaaata
     2281 aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt tgtgaaaata
     2341 gcttctcaca tttttgctta aatttgactt cataccgaga aactggcttt tgaacaccgt
     2401 ttactcggca aaaaaggttg tgacaaaaat t
//
LOCUS       DAZUKR010000209         2407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000209 DAZUKR010000000
VERSION     DAZUKR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.214"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACG89C_002762"
     CDS             5..1150
                     /locus_tag="ACG89C_002762"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS4901243.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACG89C_002763"
     CDS             1321..1779
                     /locus_tag="ACG89C_002763"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4901244.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACG89C_002764"
     CDS             1938..>2407
                     /locus_tag="ACG89C_002764"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS4901245.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAZUKR010000210         2390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000210 DAZUKR010000000
VERSION     DAZUKR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.215"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACG89C_002765"
     CDS             197..2059
                     /locus_tag="ACG89C_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4901246.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACG89C_002766"
     CDS             2059..>2390
                     /locus_tag="ACG89C_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4901247.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZUKR010000211         2373 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000211 DAZUKR010000000
VERSION     DAZUKR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.216"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..2253)
                     /locus_tag="ACG89C_002767"
     CDS             complement(169..2253)
                     /locus_tag="ACG89C_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4901248.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          689 a          526 c          362 g          796 t
ORIGIN      
        1 atattttttc catcaaaaaa ataagttatt ttcttttatt tcattgacct tattgaattt
       61 caagctattt tgaattttct gcaaaattaa aaacattagt tattttataa tcctatctat
      121 tttctttaca tttttcatta agaaaaggat ataaaaaaaa gaggacgttc attcgtcctc
      181 tttttttaca tgcaagcttc gcatggcatt caaaacagct aatactgtca ccccaacatc
      241 tgcaaatact gcatctccca ttgatgcaaa accaaaagca cctaaggcaa gtaccaaaat
      301 ttttatccca atagcaaaga caatgttttg tttgacgatt ttcaatgttt tctttgctaa
      361 atggattgct tctgcaatct tcgatggttg atcattcatg atgactacat ctgctgcttc
      421 aattgcagca tcgcttccca atccacccat tgcgattcct acatctgctc ttgctagcac
      481 cggtgcatcg ttgattccat ctccgacaaa tgcggtcttt ttattgtttc tttgtaagat
      541 ttcctctaac cgctgcactt tatcttcagg aagcaattcg ctatatatct tgtccacgcc
      601 tacttgcttc ccgatatgat cagcaatttt tttcgagtcg cccgtcaaca taactgtgtt
      661 cttcacacca gcctgtttta attgaaccaa tgcgtctacg gcatcttctt tcaaggtatc
      721 tgcaataaca agataaccac tgtaactttg atcgatcgct agataaagca atgtaccgat
      781 ttcttgactt aaaggaaaag agatttcaaa tctttccatc aatttgtgat tacctgctaa
      841 tacttgtttg ccatcaactt cagctaagat cccttcacct gcaaattcct ggatattagt
      901 tgttggtaaa agcggacctg cgtagccttc taaaacagat tgagcaatcg gatgtgtaga
      961 aaactgttct gcactcgcaa tatattgcat gaatttcttt ggatcgattt ttgtatcgat
     1021 cgtctggatc gaaaagtcac ctttcgttaa tgtgccggtt ttatcgaata ccactgtctc
     1081 ggtttgcgca agcaactcca agtaatttga accttttact aatacaccga ttttacttgc
     1141 gcctccgata ccgccaaaga aactaagtgg cacggaaatc actaatgcac atggacatga
     1201 gatcactaaa aatgtcaatg ctcgatagat ccattcagaa aacgccgctc cagaaacaac
     1261 aagtggaggt aaaacagcta ataaaacggc caatcctaca acgactggtg tgtaatagcg
     1321 cgcgaattta gtaatgaaat tttcagctgg tgctttttta ctgctggcat tttctactaa
     1381 ttctaataat ttgctgatag tagaattttc ataagatttt tctacttgaa ctaaaaatgg
     1441 tttattttta ttgataaatc cgcttaatac tgtttcacca acatatacag atttagggac
     1501 tgattctcct gtaagtgctg acgtatcgac taatgattct ccttcgatga tcgttccatc
     1561 taacggtaca cgttcaccag gcttcacaag tacaaactga ccgatctgca cgtcttcagg
     1621 gttcacggtc atcaatcctt ccgtcgtctg cacgtgagct gtttctggac ggatcgccat
     1681 caattcgcga atcgattttc ttgaatgact gactgccaat ccttggaaat actcgccgat
     1741 ttgataaaat agcatcactg ctacggcttc tggatattca ccgatcaaca tagcgccaat
     1801 cgtagcaaca gacataagaa aattctcatc aaagattttt cctttgacga tattcatcac
     1861 tgccttcttc actacatcat atcctatcag cagataaatc aataaatacc ctatcaagcg
     1921 aattggttca ttaaaatcaa ataatagcaa taaagcgata cctgcaaggg aaaataaaat
     1981 ccggaacagc gtatttctct ctttttcatt tccatgttca gatacttttt ccttatctat
     2041 ttctgttact ttgacatctg gttctaattc cttcactact ttttttgttt cttgttcaat
     2101 cgtagccaaa tgatcttcct ctgcgtcaat cgttagtttt ccagaagtga agtttaccgt
     2161 tgcttccttg actccattga tcttctgaac tcctttttcg attttcattg cacagttcgc
     2221 acaatctaaa ccttctaatc tgtaactttt cattttaatg acctcctcgc ctcaacatat
     2281 gaacatctct tcataactaa ttataaatga atggttattc atatgtcaag gtatccgaaa
     2341 gaaaaaatga aaaacatctg catcggaaaa ttt
//
LOCUS       DAZUKR010000212         2347 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000212 DAZUKR010000000
VERSION     DAZUKR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.217"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..450
                     /locus_tag="ACG89C_002768"
     CDS             58..450
                     /locus_tag="ACG89C_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901249.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(623..826)
                     /locus_tag="ACG89C_002769"
                     /pseudo
     CDS             complement(623..826)
                     /locus_tag="ACG89C_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            792..989
                     /locus_tag="ACG89C_002770"
     CDS             792..989
                     /locus_tag="ACG89C_002770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901250.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1032..1607)
                     /locus_tag="ACG89C_002771"
     CDS             complement(1032..1607)
                     /locus_tag="ACG89C_002771"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4901251.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1762..2288)
                     /locus_tag="ACG89C_002772"
                     /pseudo
     CDS             complement(1762..2288)
                     /locus_tag="ACG89C_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          741 a          408 c          371 g          827 t
ORIGIN      
        1 taaatcaatt atttttcatt ttactttaac taataatgtt tatttggagg gattattatg
       61 ttagatgata gattgacgtg ttctaattgt ggtacattat tttactatga ggaagttaga
      121 aatattgtaa aacatagaaa aaaagaaatg cctatacgtt gtccaaattg caatgaagtt
      181 attaagcgaa agttgtctca tggttatttt gtcagctacg aactatatga ggaaagtaaa
      241 ataaatctga aaaaaaaacc aaaatactat aaaaatttga aaactggagc tattataact
      301 gaaaaagaat ttaaaaattt agtgactgaa gaagaaagca ggttttataa tttgtctggt
      361 aatatttgcc ctggatttat ggatcagatt attgaaaatg agtcaattga atcaattgaa
      421 aactatgttc ctgttgactt accaaaatag aaaaaataat cctgttttta cagtgaatta
      481 ttttaatttt gattttcaat aaaaatatat tacaataata atcgtaatag ctaatgaaac
      541 aatttctcaa tctttttacc actccaaaca gggagcgtca ataattttgt gtaaataact
      601 cgtccttctg caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt
      661 gttggaagcc tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata
      721 ctaggaaacg ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct
      781 tgatttgttt attgaaagat tcgattgtag aaccagaagg aatggagaac acttttgaac
      841 tttacaacaa aactactgtt gataaattaa ttccaatttt agttccaact gatttaaatg
      901 gtcatgatgt tttacttcgt tttaacggat ttttaggtga ggattataga tacctaaaaa
      961 tttctgatcc tcaaccttta acagaataat aaaaaacgtg taatcaatta tttgattaca
     1021 cgttttttat ttcaatcttc gttgtagtag tggttgcgac ataccgaaag gtaatcttca
     1081 ttacctccta ctacaatctg ttcacctgaa taaactggaa taccatcaac aatacgagca
     1141 ttcattacag cctttttatt gcaaaaatgg caaatagttt taatttcctc aattttatca
     1201 gcaaatgtca ataatgcttc ggatcccttg aagagattat tttgaaaatc attcttcaat
     1261 ccatagcaaa ttactggtac ttcctcattc attacgataa gtgatagatc aataatattc
     1321 tctctactta aaaattgaga ctcatcgacc aaaatacaat caacttttcc gtactgatca
     1381 ataatctcca ctaattctcc gtacacatcc atttcagaag taattgcaat agctggtttc
     1441 gaaacaccta tacgactctc aacttttcca aatcctgatc gagtatctat tgcgcttgtc
     1501 attatgataa ctctcttccc ttgttcttca taattatgag ccactttcag tagctcaata
     1561 ctctttccac tattcattgt tccatatctg taaaataatt gtgccattaa atttattccc
     1621 ttcttttaca attcaattat tccttaattc catctgcttt tatgcagctg ctcgtttatt
     1681 ccttgatatg tctataacat ctactatccc taaaaccaat ttataagcca ttagagatgg
     1741 tattcctgaa catctctcat attgtttttt gagtggtata tcatctgaaa aactgaagga
     1801 atcatgaaaa tagcgacata cttatcattt agagttctaa ctttaaatgc atttacttct
     1861 tcagtcagca ctttagtcat gttggacatg gtttggggcg tgtagtgatg cccatacatt
     1921 ttctcaatca agtctgcgat ttccgacatg gtaatacctt ttcggaagag gtgaatgacg
     1981 gtctcctcta atgtgtcatt cgtccgtcta taagcaggaa cagtctgttg cttgaactcg
     2041 ccattgcggt cgcgcggaat ctggagatga agttccccat actcggtctt gaccgtacgg
     2101 tcataggagc cgttacgaga attacccgtg ttaaaaccaa tgcgatcgta cttttcgtaa
     2161 tcgaggaaag ccgttaactc cgttttgaga agcgtattga cagctttttc caaatggaca
     2221 cggaataatc cattcaaatc gcctttgttc gctagagtct ttaggatttc tgtagtaaaa
     2281 tcgttcatat ggaagtcctc ttttctgtga atgtgttgtg gtaactctat tctacagaag
     2341 ggacttc
//
LOCUS       DAZUKR010000213         2243 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000213 DAZUKR010000000
VERSION     DAZUKR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.218"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..252)
                     /locus_tag="ACG89C_002773"
     CDS             complement(<1..252)
                     /locus_tag="ACG89C_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS4901252.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKL"
     gene            345..694
                     /locus_tag="ACG89C_002774"
                     /pseudo
     CDS             345..694
                     /locus_tag="ACG89C_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(966..1256)
                     /locus_tag="ACG89C_002775"
     CDS             complement(966..1256)
                     /locus_tag="ACG89C_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901253.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1464..2156)
                     /locus_tag="ACG89C_002776"
     CDS             complement(1464..2156)
                     /locus_tag="ACG89C_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4901254.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          633 a          425 c          333 g          852 t
ORIGIN      
        1 taatttccat agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct
       61 tgaactccat gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct
      121 acttgcataa actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa
      181 gataccccaa atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt
      241 atgtttgttc atttttatac tccttcttat atttcctaat agagtataac atagctttaa
      301 gcattactta aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat
      361 ttgatgcaaa agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt
      421 tttcttagtt aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa
      481 gacatacaat catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa
      541 aatccaaatg ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc
      601 ttcaatttac ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca
      661 ggaacgttta aaatcctaca attccactac ctgagactag taatgactag tctcttttta
      721 tggcctctga tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc
      781 tttaagcaat atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa
      841 tacatgtagt tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc
      901 ccatcgtctg catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt
      961 gttttctagt ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca
     1021 tattctacca caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt
     1081 ctattattat aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag
     1141 ttcaaactat gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt
     1201 ctggcactaa cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc
     1261 ttcacctcaa taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta
     1321 tctttttgac tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat
     1381 gaaggacatg tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat
     1441 ttaggtttac ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat
     1501 ttatcagctg tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc
     1561 aagcttgagt tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgagtt
     1621 tctacatttg ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta
     1681 ttttgtaatt ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc
     1741 agatgattga tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt
     1801 tgtttgcttt gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct
     1861 gctgccgatt gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca
     1921 tctgcctttg aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta
     1981 agcgcttgct ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta
     2041 tcaatatctg tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag
     2101 ccaattagac atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc
     2161 atagctcctt aatccaatac ttttacatgc taaaagtaag tgatctcaaa cacaatgtca
     2221 agcgttttta aattacatgt gta
//
LOCUS       DAZUKR010000214         2242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000214 DAZUKR010000000
VERSION     DAZUKR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.219"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..491
                     /locus_tag="ACG89C_002777"
     CDS             90..491
                     /locus_tag="ACG89C_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4901255.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            485..730
                     /locus_tag="ACG89C_002778"
     CDS             485..730
                     /locus_tag="ACG89C_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901256.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1054..1275)
                     /locus_tag="ACG89C_002779"
     CDS             complement(1054..1275)
                     /locus_tag="ACG89C_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901257.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1437..1509)
                     /locus_tag="ACG89C_002780"
     tRNA            complement(1437..1509)
                     /locus_tag="ACG89C_002780"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1474..1476),aa:Lys,seq:ctt)
     gene            1585..1815
                     /locus_tag="ACG89C_002781"
     CDS             1585..1815
                     /locus_tag="ACG89C_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901258.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            1859..>2242
                     /locus_tag="ACG89C_002782"
     CDS             1859..>2242
                     /locus_tag="ACG89C_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901259.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQ"
BASE COUNT          860 a          298 c          366 g          718 t
ORIGIN      
        1 agaaggatat ttttttctag ttttttcgaa aaaaaggcgc tttctgattg ttgtattagt
       61 ggaaagggga aaaaaggagc atatttatca tgcaacaaaa ttacaatgag aaatctatac
      121 aacatcaatt tgatgcctac tgtaaaaaag tattacgaaa cgaaactaag tctattcgaa
      181 aacgaaacat taacataaca gataatgagg aaccattgac gaattcaaat gaaggtaagt
      241 actcccaaac tcattttgat gaacaagatg tctatttctt atttggaatg gaaatattaa
      301 tttcagatca aaagttatcg gcagccattg atcatttatc ggaaataaga agaaaggtta
      361 tcctacttta ttattttgca ggttttaatg atactgaaat cggtaaaatc ctcaatatga
      421 gtacaagtgg tatctggtat caacgaaaga aggcagttga acaattaaaa atggggtatg
      481 acttatggta gacaacccca aaaaacggca gtatccatta gttcctctat ccattatcca
      541 atctgctcta gaagaagatc caatttcaat caagatgata ctcaaacatt atcatagtta
      601 tatagctaag ttatgtttga caaatggatt taatgaggcg gggcaattta ttacctatgt
      661 agatgagtat atgctaagac agctagaaat taaattgata gaagcgatct taaaatttaa
      721 aattaactaa ctaaaaagtc ccctttccac ttattctgtt taaaagaatc ttctaatagt
      781 taaaggatta gtagattctt ttaaacagaa aacctattga tcattgaaat ttaaatacat
      841 tctatcagta cgttaacact tcaaggagca tattttagtt gcgccatgat ttcttactgt
      901 aaagaaggag cgataaacat tctaaaataa tttttatagt caataaaaag cgatgatttg
      961 aagttgggat aatgatactt ccgtaaggct cgccgtaaac ttggagagag gtgaaattcc
     1021 tatgtgcttt tgacaaaagc agctgattaa gaattatttc catatgttga tttcaacatt
     1081 ttttctatta tttttaagac caaattcagt gaggcaaata gcatcatgta aatttagttt
     1141 ttctattgaa cgttctccac tttttagttt ttttatagta ctcaaattta atcctgtatg
     1201 tttagagatc tcatttgcag tatggttttg taaagtttct cggattattg ttatattagc
     1261 aatttcatta cgcataatta tgttcctttc ttatcaaaaa tatcaatgac tacagtgggc
     1321 ttttagtgaa aaaaaggggt actacagtct cttttgaaaa caaaaaaata cagtatatac
     1381 gttttctaaa tgctaagcac caacgtttac actgtattta agggtttggc acccaatggg
     1441 ctgtgagggg ctcgaacccg cgacccgatg attaagagtc atctgctcta ccaactgagc
     1501 taacagccca aattaatact ttgttattct aacacctaaa ataaaaaagt gcaatactta
     1561 ttatcagaat tgagagtgaa ttttatggat tttcaagatt atgaaaaaat atttgaaagt
     1621 tatccatcga tattgaaaat tactgattta actaaaatgt taaatattgg aaaaaataaa
     1681 gcttataatt tgctgaaaga tagatcaata gaaaatataa aaattggagc aaggtacaaa
     1741 atacctaaaa aagctgtcat tgagtatata ttgaaaatac aagatgaagg taaaaatcat
     1801 gctataataa aatagtcaaa atttttgccg atatttcagt ttaagaggag agattgaatt
     1861 gaaattaaga gcaaacataa ttgaaaaaag aggaacatta caagtagttg tatcttataa
     1921 agactctaac ggaaaaatta gacaaaagtg gaaagataca aaattaaaaa agaaaggaaa
     1981 taaaaaacga gcagaaaaaa ttagagatga atttttacta gaattggaaa gtcaattagc
     2041 tatggaatgt gaatgtgcta atccaaattt attgttttat gactatttgc ttaattattt
     2101 ggaagtatca aaaaaacaag tagcatttaa cacatatgtt gggtataagc attacgtcca
     2161 taatcgaatt tacaagtttt tttatcctaa aaaaattaaa ctaaataggt taaagcctta
     2221 tcatttacaa gagttttatc aa
//
LOCUS       DAZUKR010000215         2236 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000215 DAZUKR010000000
VERSION     DAZUKR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.220"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..256)
                     /locus_tag="ACG89C_002783"
     CDS             complement(32..256)
                     /locus_tag="ACG89C_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4901260.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(253..546)
                     /locus_tag="ACG89C_002784"
     CDS             complement(253..546)
                     /locus_tag="ACG89C_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901261.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(583..720)
                     /locus_tag="ACG89C_002785"
     CDS             complement(583..720)
                     /locus_tag="ACG89C_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4901262.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(722..1168)
                     /locus_tag="ACG89C_002786"
     CDS             complement(722..1168)
                     /locus_tag="ACG89C_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901263.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1165..1314)
                     /locus_tag="ACG89C_002787"
     CDS             complement(1165..1314)
                     /locus_tag="ACG89C_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901264.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1328..>2236)
                     /locus_tag="ACG89C_002788"
     CDS             complement(1328..>2236)
                     /locus_tag="ACG89C_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901265.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          586 a          476 c          383 g          791 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt attcattttc ttctctccct aaacaaagtt ttgatttgtt gcgtgtgttc
      301 caccaatttt tctgtatgtg tatctaatct ttcatcgtgt ttctttagtt cttcatgaat
      361 catcaatcga tctgatttgc tcgattctaa atctttagtc agcaaatcta aattgtggct
      421 tacttttgaa agagtctcag taattttcga gaaagatgca gcaatcggtc taattactaa
      481 taaaatcaaa gaaacgatag cggttattga tcctgctatc attccccatt cccctaaatt
      541 aatcatgtga caactccttt accttaaata aaaacgcatc aattaagatg cgctcttatc
      601 tttattaatg attttatctg cttcttcgtc tgtaatgcat agtggaacga atagtcgaac
      661 ttgatcgtca gtaaaacagc cccaatcata catcattttc acatcgctaa aactaaacat
      721 actactcacc tccttttgaa gctggattta gttgctcttt aatttctgaa atgtctttgc
      781 tattttgtaa cgaagcaagc atcatttttg aattgatttg tgctaaactg tcagcttttt
      841 ctttcaatgc agtattttcc tgtttaatcg ctacatcgct tagcatgagt ttggcattta
      901 gctgttttag gttgccgttt tcatgttcaa gagcctcgta catcgctttg agattgttta
      961 aatcgttgtg atctaacgcg ttcgctaaaa taatccattg gttcaattta ggatcaaaca
     1021 tctgatcagc aatcgttagc ggttcgccat ctgaacgaac cccttcgagc ggtggctgat
     1081 ctgtgtaagg aacggataca agcatgtcgt ccaatacttt tcctgcatac tctccgccag
     1141 tacggccata tttccaaatg tttttcattt atttccctcc cagtaattga atttcggttt
     1201 ccaatttgga atcggtggtt cgacttctgt gcattcttcc ggtaaatgtt cttcgtcatt
     1261 cacaatgatt tgctcgaatc cgtaaggctt gattgatttg taaactactt tcattagatt
     1321 tcctccctta aattgaataa gtgataacaa atgaataagc tgatccataa cttgagtttc
     1381 ttcgccattt aatggctcca tctgcaccaa tagataactg agcactgttc aaagtagaac
     1441 ggtctatcga gccaaccagt tgctcaaaac taattggtgg ccgatagcct tctggaattg
     1501 ttagtatcgt tgaatcattt ccaccactgc ttttcccatt caacgcaaaa aaataaatag
     1561 tgacggtttt tccttcacga taaagctttg ctgatccggt attcccgttt gtaactgtta
     1621 aggttacgac ttcatatttg ttgtcgtctg atgtcaatac agattttcct tttgattgaa
     1681 taccatcttc gaaatttttc aatccttcga tggattgcgg ttcagtcaaa ctaaccgtat
     1741 cattcaagcc tttttcagta tattcaggcg tgatgtccca gctgtagtcg ttcggattgt
     1801 tgctatcttt caatccttca ccaaagtatt taaactgact aatattcggg gttcgggtgt
     1861 cgcctttttc aattttgagc cagtcaattt ggcatgcgcc ggccgtttct ttaggcaact
     1921 gaaaaatgcg aaggtcttta ggcaaacccg gttcaagttt cgttggtgtg aatgttagag
     1981 accatacgtc tgtcaatccc tcaactggtt ttagttctcc aaaattaacg ttccaataat
     2041 tatatgctac aaaggtttga cttgcgggtt ttgttccttt aagcgtgata gtatacgttt
     2101 gacctattat aagctcttct tccgtgttac ctttatatat ttcgtacgcg ctagatttaa
     2161 ttgggaacgt cgtaccttta tttgcaatat tctcacccaa cgatgtttta cctacccagt
     2221 aagggtcatc tagtaa
//
LOCUS       DAZUKR010000216         2233 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000216 DAZUKR010000000
VERSION     DAZUKR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.221"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..544
                     /locus_tag="ACG89C_002789"
     CDS             <1..544
                     /locus_tag="ACG89C_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4901266.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="ACG89C_002790"
     CDS             complement(900..1472)
                     /locus_tag="ACG89C_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4901267.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /gene="fic"
                     /locus_tag="ACG89C_002791"
     CDS             complement(1488..2093)
                     /gene="fic"
                     /locus_tag="ACG89C_002791"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS4901268.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt cta
//
LOCUS       DAZUKR010000217         2226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000217 DAZUKR010000000
VERSION     DAZUKR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.222"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..591
                     /locus_tag="ACG89C_002792"
     CDS             106..591
                     /locus_tag="ACG89C_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901269.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            1047..1460
                     /locus_tag="ACG89C_002793"
     CDS             1047..1460
                     /locus_tag="ACG89C_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS4901270.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            1597..1857
                     /locus_tag="ACG89C_002794"
     CDS             1597..1857
                     /locus_tag="ACG89C_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901271.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            1975..>2226
                     /locus_tag="ACG89C_002795"
     CDS             1975..>2226
                     /locus_tag="ACG89C_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901272.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLT"
BASE COUNT          691 a          355 c          369 g          811 t
ORIGIN      
        1 atgaaccttc cctttattgg gaaggttttt tcattttaaa ccatctgctt ttctttgttt
       61 aagtgatata ctgaataaag ataatctatt ttaggaggag ctatcatgca taaagggatt
      121 tacgcaggct tgttgggaac tgtaatactt ttttctgcct gtggatataa aacaaagaat
      181 tctgttaaag aatcttcagc agccatttca acaactgagg ttggttcaac taaaagtact
      241 tctactactt ccattactaa tacttctact ggtaatgtag attcgcaaca tagccaatca
      301 agtattcagt cgtttagcat ttcttcaaca tctgaaatgc aagaaagcac tgtaccttct
      361 cttccattag aaaactctga caaagcactt ctatttctta ttgcccaccg ttccgaatta
      421 caaagtgacg atattgttat tagcttttat caaaaaattg atcgagatta tttgttcaca
      481 gcttccagca aacaaatccg ttcccaagga ggatctggaa gcgttggttt ttatcgagtg
      541 tcacccgaag gatcgattac gatgacagat gcaaatggaa caccattttg attagatgat
      601 attatacgga tttacagtac tagcaagtca ttattggtat cattttttct gagaaagtta
      661 tacggagtat aaataagcat ttgcattaac ctaataattc gttacagcta tttctcttat
      721 gaatatactc acttctctac ttgctctctt gataaatttt tggtatctac ttcattttcg
      781 gtcaactgag ttaatgaaat ttattgtcgg gcggttttct agattcaatt tttctatatt
      841 tggatagaag aatttacttt tttacctttt gacatatttt cttcttatta ttttaatgat
      901 ccctttttca aattagaaag atagcctttg tatcgtatta gatgaaatat tcacaaagct
      961 aaccattata cttgcatttt tttcaaaata gagttattac gtaaataggg ataaataaaa
     1021 aatacttatt gaaaggatga tcaaaaatgg caaatgtacc tagcattcgc gacatgttcc
     1081 ctgatttcaa tgacgtgttt agtccagcat tcaatgattt tttaggtgtt tcaagttatc
     1141 caaaagtaga tttagtcgaa aatgaaaaag aatacaaact aactgctgat atgcctggct
     1201 gtgacaaaga agacacgaca gttgaatatt cagataatac tttgacgatc tccgccaatc
     1261 atgagtccca tacagaagat aaagaagatg gcaactatgt acggaaagaa agacattcgg
     1321 tctcttacaa acgttcattc tatcttccta atgttgatga agaaaaaatc actggaacct
     1381 ttaaaaacgg tgtgctgaaa ttggtattac ctaaaacagc ttatcagcca aaagaaacaa
     1441 aaaaaattga gttaaattag aacttatcgc ctctcgatga atgaggggcg atttttatat
     1501 atttggataa gaacgagtat gtttaaattt tcaaaatgag caaaaaaata ttttgttttt
     1561 acgataaaga tgttatgatc tctcttggta aaaaatatgg gagttgatca tttgattgtc
     1621 ttaagtaaac ttttgttttg gataccattt atcataatga ccagtgcttt cttttactta
     1681 ttcaaaaggc ctaacaaata cttactctta tctgcttgtc cgatgatcta ttttatccat
     1741 aagattagag gattttatta ttacgactct ggtatctcat tgttttgcta cgaaatcggc
     1801 ttattagctt cggccatctt tttcatattt gtgttagaat atttcagaaa aaaataaact
     1861 tccagtcagt tttggctgga agttttttat ttggtgcaga tcttttcagc tgttattcat
     1921 acaagttgat ccatttttct attattaagt acataccatt gttactcttt gaatgtgggg
     1981 gagaaaaaaa taaaaggagc tggtattttg ttagttatga gtaaagcttt gtttttactt
     2041 ccctgcacgt tcatgtttat tgttttatgt tatttcaaaa gaagaaaaaa atggtgctgg
     2101 atcgatgttc tactattcct tccctttttc ccgcttattt attttcttga agaaatatgg
     2161 gatgatatgg catgtgattc tgcttttctt tttagtcatt cattggggct tatgatctct
     2221 cttacg
//
LOCUS       DAZUKR010000218         2205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000218 DAZUKR010000000
VERSION     DAZUKR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.223"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..>2205
                     /gene="acm"
                     /locus_tag="ACG89C_002796"
     CDS             60..>2205
                     /gene="acm"
                     /locus_tag="ACG89C_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS4901273.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFL"
BASE COUNT          861 a          348 c          430 g          566 t
ORIGIN      
        1 atttttgaga gatgatatag taggttttat ttttagttaa aataggggag atagaaaaaa
       61 tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac
      121 tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt
      181 tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg
      241 acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag
      301 gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg
      361 ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa
      421 acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata
      481 ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa
      541 atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca
      601 tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt
      661 atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa
      721 gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg
      781 gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata
      841 attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt
      901 ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt
      961 ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata
     1021 tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata
     1081 tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac
     1141 agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta
     1201 tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg
     1261 attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg
     1321 gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt
     1381 ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa
     1441 acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag
     1501 tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc
     1561 aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg
     1621 ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg
     1681 aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg
     1741 acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca
     1801 caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg
     1861 tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa
     1921 aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga
     1981 cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag
     2041 gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa
     2101 caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa
     2161 tcattggttt attgatagta actatctgtg ctgggatatt cttac
//
LOCUS       DAZUKR010000219         2191 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000219 DAZUKR010000000
VERSION     DAZUKR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.224"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG89C_002797"
     CDS             complement(<1..914)
                     /locus_tag="ACG89C_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS4901274.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG89C_002798"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG89C_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4901275.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG89C_002799"
     CDS             2006..2176
                     /locus_tag="ACG89C_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901276.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          743 a          384 c          420 g          644 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt a
//
LOCUS       DAZUKR010000220         2161 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000220 DAZUKR010000000
VERSION     DAZUKR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.225"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..839
                     /locus_tag="ACG89C_002800"
     CDS             <1..839
                     /locus_tag="ACG89C_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFS4901277.1"
                     /translation="KSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACLYRNNKNV
                     QVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGRENT
                     IQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKIAD
                     LVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVI
                     SGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDD
                     IL"
     gene            836..>2161
                     /locus_tag="ACG89C_002801"
     CDS             836..>2161
                     /locus_tag="ACG89C_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS4901278.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMI"
BASE COUNT          770 a          254 c          382 g          755 t
ORIGIN      
        1 gtaaatcaaa tcgatatgcc tattatttga aaaactgtaa aagtgaatat tttgatgaat
       61 ttatttgttt ttcaatggga gaggtttggt tggaaggaat atatgcttgc ttatatcgaa
      121 ataataaaaa tgtacaggta tcaattttag aagaaggtat tttaagctat ggagttgaca
      181 ttattccaac agcaagaaca aaagctatcg cagtactcaa ttacttaaaa aaaaggactc
      241 cactatataa caaaagctat aatttttatt gtttttttcc taatatatat acaggaagag
      301 agaatacaat acaaattcca ttagttacag ctgggtcaag aaccgcattg caactttcgg
      361 atatttttaa ccctatgata gatatttata aagaaaaaaa atatatattt tttacgtctg
      421 tttatgattt tgagggcgga gaacctgtcg gagaatatga attagtatgc aaaatagcag
      481 acttagtagg aaaagataat ctaattgtta agacgcatcc tagagatgta agaacaattt
      541 ataaagataa tggatttatc gaggataaaa attcatcaat accttgggag gcgattcaat
      601 tgtcaggcga ttttaacgat aaaatattta tgacaattaa ttcaggaagt gttatttcag
      661 gtagtacaat gtctgcaaaa cctgtacgca cgtattatat gtataaattg tgtaacatag
      721 aaggaaatcc gtcttgcaaa aagaatgcgt atgatattga gcgattgctt aagcaaaaaa
      781 cgatgaaaaa cattttaaag aatgtgaaaa tagcagaaag cttagatgat atactatgag
      841 caaaactaaa tatttaatta aaaatatggg aatattaaca ataagtaatt tttcttcaaa
      901 aattttagtt tttttgttag tccctttata tacaagtgta ttatccacaa cagaatatgg
      961 cacatatgat ttgacaattt caacagccac attattgttt ccaattttaa ccttaaacat
     1021 tgtagatgct gtgatgcgct ttttaatgga taaagataaa aataaaaaaa atatagcatc
     1081 tatagcgatt aaagttatat ttttaagcgt gatagtcttt ggatccgtaa tgtatttgtt
     1141 aaatattaca aaattatggc aagatgttta tggattagaa aaatatattt ttttgtatta
     1201 tatatcctat gtatggaatc aatttctaat acaatttgct aaagggttag aaaaagttaa
     1261 agctatgggc atagcaggta ttatcagcac aattgtaaca ataattgcca atattttctt
     1321 cttgcttatt ctgaaatggg gattaattgg ttttttcatt gcaaatattt tatcacagat
     1381 tatttcagct acttatctgt cagtattagt gagaatatgg gaatatatcg attttgaggc
     1441 taaagatcct cagctaaaga gagaaatgct aaattactcg attccattaa ttgcaactgt
     1501 tgttggatgg tggattaata gtacagctga taggtatgtg gtttccttta tagtaggtgt
     1561 ctcggcgaat ggcttattgt ctgtggcata taaaatacct caaataatta atactttgca
     1621 gggaatattt atacaggcct ggcaaatttc tgcaattaaa gaatatggag ataatgagac
     1681 tgcaaaattt tatggaaata catttgttat gattaatttg ttaatgtgca taacctgttc
     1741 aagtcttatc atattaacta gaccgttttc acatattctt tatgcgaaag acttttatga
     1801 agcgtggaaa tatgttccat ttttattaat agctaatgtt ttaaattgtg catcaggtct
     1861 gttaggacct atcttatcag caaaaaaaga ttctaaatcc atgatgtggt ctgcaatcat
     1921 aggggcatta gttaatgtga ttatgagtgt agtccttgtt tataagatag gggttcaagg
     1981 tgccacaata gccactgtta tatgttcata tgtaatatat gttgttcgta aacgagctgt
     2041 gggaaaagat atttttataa agcattatca tattgttgtg gaatcttggg gattattgtt
     2101 tgttcaggca atcatagaaa tatattttaa aaattatgta atagaaatta taattatgat
     2161 t
//
LOCUS       DAZUKR010000221         2147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000221 DAZUKR010000000
VERSION     DAZUKR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.226"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..535
                     /locus_tag="ACG89C_002802"
     CDS             2..535
                     /locus_tag="ACG89C_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901279.1"
                     /translation="MSFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSIL
                     TLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCS
                     NRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAF
                     SFAREDEDNPKTYDILA"
     gene            complement(596..961)
                     /locus_tag="ACG89C_002803"
     CDS             complement(596..961)
                     /locus_tag="ACG89C_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS4901280.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1037..1282)
                     /locus_tag="ACG89C_002804"
     CDS             complement(1037..1282)
                     /locus_tag="ACG89C_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4901281.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1352..1606)
                     /locus_tag="ACG89C_002805"
     CDS             complement(1352..1606)
                     /locus_tag="ACG89C_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901282.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1770..2093)
                     /locus_tag="ACG89C_002806"
     CDS             complement(1770..2093)
                     /locus_tag="ACG89C_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901283.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          806 a          362 c          373 g          606 t
ORIGIN      
        1 tataagcttt tttaaagaat atttagaaat tattaatttt cgtctgaaaa caaaaaaaga
       61 aagcgttatt attttagtct attttcaggg ggggaacctt atgataaaaa aactattttt
      121 actatcaata ttaacgctag caatggcagg ttgttctgac tctggcttag caactgaact
      181 gatcacaaaa tcaactgaag ccacgacaga ggagttaaaa tgtggaaacg atcacagtga
      241 ctacagaggc ttaaatcgac gatgcatggg aatattaggc agaagcaatt gtaagcatcg
      301 atgtagtaat cgaagaaggt gtagaagaag caacagaaaa atcaaaagaa agatcaaaag
      361 aagcaatctg tgctgtgcat cgtatacaga cgtcgaagat gcggtacttt taaaccacgt
      421 caggagatgt tcatttatgt ctagggaagt tggcgatgaa ccaaaactag agtacgcttt
      481 tagttttgca cgtgaggatg aagataaccc taaaacttac gacattttag cttgatctaa
      541 gatggttaaa gctttttaat gaagtaagag gagatagaag gcttttctag ttatattagg
      601 taagataaat tcctttttgc tccaaatttg ctaaacattc tgctagataa acttcatcgt
      661 gttccggata aatcggcacg ttgcgctcta cagtaggtaa atcaaaaaat gcttcgcatg
      721 atttgccacg atacatagga tttttccact ctggtgctgg gctgtaacca cgtttttcca
      781 tttcatccat gacaagctga tgatattgga ataatctata aggagaatag tgaaaaacat
      841 aatccacagt agcatgtttc tttccccagc catttcctct taatgcacag cattcccgat
      901 gttgtcctaa aagctgttga cgaggaagct ttgaaatcaa tttttcatgc cataatcgca
      961 tagaaatgtc tcctttatat atagattgtt aaccttagac gttaaatgaa aaaaccagca
     1021 ccaaggctgg aaaaaatcac ttcagtttgc ttgctgtttc tgagcgacca attaggtaat
     1081 caacggaaat atcgtaaaag tcagcgatat agattaattc aatgatgctt ggaatttctt
     1141 ctccttcttc gtacttacga tatttgaata aaggaatact taacgcgcta gaaacagcta
     1201 cttgagataa agatcggcca gttcttaaga aattcagacg acaacgtaaa gtatccagta
     1261 aaatagcttc ttcgatactc atttaatcac ctttcattat agtagaatag ttataatgac
     1321 tatagaaagc tacacatgca atcaatagta ctcaatgttt tttatttaaa cgtatcaaat
     1381 aaagaatgac gatcaataaa gacaaaacca gtcctgataa ttcttctata aaaacactat
     1441 cgggttcaaa gtagttgtaa ttaaaaactt tccacataaa gtagatagcc ggaagagtca
     1501 aaatagctaa ataaaaccgt tctttgttcc aattcataag ataaacaata cttgcgacca
     1561 gtgcgaccgc aggcacccaa aacaaaactc tcaccaacgt aatcatgtaa cggactcctc
     1621 aacgattcat ataataaaag ataacatgtt attgcataca tcacaaatga aatgctagga
     1681 aaataaaaga agaatagatc aatgaaaaat gaaaaagcag aacagttttc tgctgataag
     1741 caggaactgt tccgctgaat gtgtcttatc tatcgagaaa aaatcaatgg attgatatct
     1801 aagtaattca atacatataa aaagaggacg atcgcattca ctaaaaagga aagaatgatg
     1861 atcccatagc gaatggaaac atatttaaca aatacttttg tttgaatacg ggcatatctt
     1921 tttcctttat agctgagcat catgacaaga agcaaaaggg taatcacacc ttgaaaaatc
     1981 attccaataa acagaattcc tgaggtttga taatttaatt ggagaaaata agctgctgcc
     2041 gcaactaaat cgaataaaat cgcaataaat ggcaaaataa tcactcctaa cattgagaat
     2101 aaataaaaga ataacatttt ttctaaaata agcaaataaa aattaaa
//
LOCUS       DAZUKR010000222         2092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000222 DAZUKR010000000
VERSION     DAZUKR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.227"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            705..1037
                     /gene="traE"
                     /locus_tag="ACG89C_002807"
     CDS             705..1037
                     /gene="traE"
                     /locus_tag="ACG89C_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS4901284.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1656..1997
                     /locus_tag="ACG89C_002808"
     CDS             1656..1997
                     /locus_tag="ACG89C_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4901285.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          735 a          279 c          434 g          644 t
ORIGIN      
        1 ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg ttaaaagaaa
       61 taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt ttttgagtta
      121 taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac
      181 ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg
      241 ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt tggacaattg
      301 aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt
      361 catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc
      421 cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa
      481 ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta taaaaatgtc
      541 cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg
      601 ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag
      661 aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa
      721 ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca
      781 cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc
      841 agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt
      901 taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa
      961 acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg
     1021 ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc
     1081 ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat aaggagtaga
     1141 cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg attggtaacg
     1201 taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt
     1261 aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgt
     1321 ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact catggaataa
     1381 ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac
     1441 atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc gctgtctact
     1501 agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt tttaagtaaa
     1561 cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat ataaaacata
     1621 gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac
     1681 gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa ggatttgtga
     1741 cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac gaggatgatt
     1801 tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg
     1861 aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg
     1921 aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt gaatttgtag
     1981 cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct tagaaggagt
     2041 cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat tt
//
LOCUS       DAZUKR010000223         2040 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000223 DAZUKR010000000
VERSION     DAZUKR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.228"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACG89C_002809"
     CDS             <1..1872
                     /locus_tag="ACG89C_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFS4901286.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DAZUKR010000224         2003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000224 DAZUKR010000000
VERSION     DAZUKR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.229"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG89C_002810"
     CDS             <1..378
                     /locus_tag="ACG89C_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4901287.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG89C_002811"
     CDS             426..1826
                     /locus_tag="ACG89C_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4901288.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZUKR010000225         1992 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000225 DAZUKR010000000
VERSION     DAZUKR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.230"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG89C_002812"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG89C_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4901289.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG89C_002813"
     CDS             852..1040
                     /locus_tag="ACG89C_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4901290.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG89C_002814"
     CDS             1053..1610
                     /locus_tag="ACG89C_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4901291.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG89C_002815"
     CDS             1642..1884
                     /locus_tag="ACG89C_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4901292.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          554 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ct
//
LOCUS       DAZUKR010000226         1914 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000226 DAZUKR010000000
VERSION     DAZUKR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.231"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..1528
                     /locus_tag="ACG89C_002816"
     CDS             62..1528
                     /locus_tag="ACG89C_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4901293.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          589 a          328 c          393 g          604 t
ORIGIN      
        1 tgttaaaaat tgaatatgat tacatctgga taacaaattt ttatcataaa ggagagaacg
       61 aatgaacaac aaacaattac gctggtttac tgttgcgtta attgcattta acatggtttg
      121 gggcatgggg aatgtagtta ataactatgc tcagcaagga gtatcagttg tcacctcttg
      181 gctattgatt ttggccattt attttattcc atacgcattg atcgttggac aattaggatc
      241 agccttcaag gatagtaaag gaggcgtcag ttcatgggtc gaaaatacga catctaataa
      301 acgactggca tactatgccg cttggactta ctgggtcgtc catattcctt atttggcaca
      361 aaagcctcaa gcaattctga ttgctggtgg ttgggcagtc caaggaaatg gaaatatcgt
      421 aaatacgatg tcagtacaaa tggtagcagt tatttcactt attatatttc ttgccttttt
      481 atatttatca acaaaaggac tttctacttt gaaagtgatt ggcggactgg ccggaacagc
      541 gatgttcgtc atgtcgatct tatttattct tttagctgta acggcaccgt tcatcaatcc
      601 aacgatggaa tttgccacac cacatatgaa tcaagtgaaa acatatattc caacatttga
      661 tttcagttat ttcacaaccg tttcgatgct agtatttgca gttggagggg cggaaaaaat
      721 atccccttat gtcaactcta caagaaatcc tgcaaaggaa tttcctaaag ggatgatctt
      781 cttagcagca atggtaggga tttgtgctgt attaggctca gtcgcaatgg gtatgctgtt
      841 tgctagtgat aatattccta aagacttgat ggcaaatggg gcatacagtg cttttcaaat
      901 tttaggtaat tactatggta tcggtaactc attgatgatc atttacgcat tgacaaacat
      961 gattggacaa atctccgcat tagccttttc aattgatgca ccactaaaaa tcttgttagc
     1021 agatgcggat ccagaatttg taccaaactg gctacgtaaa cgtaatcgta aaggtacatt
     1081 ggtgaatggt tatcttctga caggtatact agtaggaatc attattttgt tgccgatttt
     1141 tggtatcaaa gatatgaatg aattagtaaa atggttgacg aacttgaact cagttgtcat
     1201 gccaatgcgt tacctatggg tgttcttggc atatatgatg ttgaataaag cttataagaa
     1261 attcaattct gaatataaat ttgttaaaaa tccaaaaatc ggttttatct ttggtgcttg
     1321 gtgctttgcc ttcactgcgt ttgcatgtat cctaggaatg gtgccaaaag tagattatac
     1381 tgctgatcct aaagcatggt ggttccaatt atttacgaat atcatcacac caatcgtctt
     1441 gatcttatta ggtatgatct tgccagcaat tgctcgtcga gacaaaaaca aaaaaatcac
     1501 tgaaccagaa acagtggttc agccttaaaa tagaaatact tgttacaaga ggttgtgaca
     1561 gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc aaatttttgt
     1621 gaagtttcgg cttgtttttc gaagaagcta cttctggaac accgtgaatc agaggttgtg
     1681 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagccc ctcatatttt
     1741 tcgtttggct gggcttatta ccgcagatgc aagctttgga acaccgttta aggaagaggt
     1801 tgtaaaatca gcgatttgac ctgagtatta gagaaacaaa cggaaaaata gctcctcata
     1861 ttttgccgct ttttgagcta atgctgaagg tcagctgatt gaacaccatt tatt
//
LOCUS       DAZUKR010000227         1880 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000227 DAZUKR010000000
VERSION     DAZUKR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.232"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..285)
                     /locus_tag="ACG89C_002817"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACG89C_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACG89C_002818"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACG89C_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          656 a          265 c          248 g          711 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt
//
LOCUS       DAZUKR010000228         1848 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000228 DAZUKR010000000
VERSION     DAZUKR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.233"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            566..>1848
                     /locus_tag="ACG89C_002819"
     CDS             566..>1848
                     /locus_tag="ACG89C_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4901294.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          605 a          353 c          344 g          546 t
ORIGIN      
        1 ttttcagctt ttttattctt ggaataacga ggtattgttt tcgataaaaa aagatggtgc
       61 agacaaatta tgataattaa gcatactatc agataactca aaatgaagcc ttctttcaaa
      121 aaattattca aaaccatata ttgtattttt tatttgataa taacaatggg attattaaga
      181 agtggcacta aataaaagac tagtgtgtat ttagccgatt taattataaa caagtaaatg
      241 gataaagtat atgtaatgat aaaagaaaaa tggattttct aatttttaag ctaaatgtta
      301 gatcacacat gtatatgctg gatagtctta taaaaaatca tgtaatacag cttggtacat
      361 gatttttatt tgtagctgtt ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt
      421 ggtttctttt ttaggatcta taatatctag tgttggtgac gccttctgtt gccaatactt
      481 tttctttttt ctaataaacg caaacagaca ccttgaaatc ctttagaata aagtcgacga
      541 aaaccattca aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga
      601 tattctagac ctaaatctta cctttcatga agatgccttt cgcaaagagc gcattaatga
      661 cgaaacatgt tttgtttttg atggcacatt gacctatcaa ccagaggaat gcttccattg
      721 ccactatcaa aataagcaaa cgatcattaa atggggctgg aaaaaagtat ccatcttatt
      781 gaatgatgtc agtaactaca agaccattgt gcggattaac aaacagcgct tcaaatgtaa
      841 acactgtgga aagacttttt tagcagaaga ttcagttagc gatcgtcgct gctccattgc
      901 ccgcagagtc aaacaagcca ttcttgagct attaagtgag cctatttcta tgtctttgat
      961 tgcacgtatg aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc
     1021 taaaaccgtt tctttgaacc agccgttacc agaagtggtg tgtttcgatg aattcaaatc
     1081 ggtgaaaaac gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact
     1141 gattgatatt gtggaaaatc ggcaactcaa tcctttacgc gactatttct tgcgctaccc
     1201 tagaaaagtc cgtgaacaag tacgtctagt ggtttcagat ttctatacac cctatcgcac
     1261 attggtgaaa gagtgttttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca
     1321 acacatcggt cgggccttta ccaaccaccg aattcaagta atgaagacct ttaaaaaagg
     1381 ggatcgtcgg tcgaagcact taaagaaata ctggagacta cttcaaaaga atgcgtggga
     1441 attgaatggt cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcgga
     1501 gatagtcgat cggctattat attatgatga ctccttaaag cgaggctacg aagtctacca
     1561 ggtttttctt tccgctatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga
     1621 agactacaag gagttgccag agcactatca accagtgttc acgaccttca agaaatatcg
     1681 aacagaaatc aaacgagcgt tgcgagttcc ctattccaac ggccctatag aatgtttgaa
     1741 caaccatatc aaagtactaa aacgaattgc ctatggcttt agaaactttc aaaactaccg
     1801 agaacgaatc tttttatatc gaggaaaata tttcaagaaa actaagaa
//
LOCUS       DAZUKR010000229         1819 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000229 DAZUKR010000000
VERSION     DAZUKR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.234"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1148
                     /locus_tag="ACG89C_002820"
     CDS             <1..1148
                     /locus_tag="ACG89C_002820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4901295.1"
                     /translation="KRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSA
                     QKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPS
                     LYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLH
                     RTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTD
                     DIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMS
                     VYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTS
                     AIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1415..>1819)
                     /locus_tag="ACG89C_002821"
     CDS             complement(1415..>1819)
                     /locus_tag="ACG89C_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901296.1"
                     /translation="LEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          637 a          316 c          283 g          583 t
ORIGIN      
        1 aaaaaagaaa atattcattt ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct
       61 ctacaggaac tattcttgga tggcaatttt acaataatga acactctcag atagttgccc
      121 atgctcaatc agctcaaaaa aagttgatac tgactaccaa tgaacaacag ctcgttgaaa
      181 aaatcgatca actattaaga aaaaatgatt atattggctc aatttacgta aagaaaaata
      241 atcgcatcat catggaaaaa ggctatggct atgcgaataa actcacgcaa caaccaaatg
      301 atccttctct ttattatcag attggttcgg ttcaaaaagc attgactact cttttgattt
      361 taaaacaagt cgagcgtggg cacctttctt tagatactaa gctggctcaa ttctatccac
      421 aaattgctgg cagccagcaa attactatcc gtgacttgct gtatatgcgc tcaggcttgc
      481 accgaactgc ttctccaacc ataccgatga ctgatgaaga agtcgttcaa tttgcgattc
      541 agcacttaaa atttacagat tatcagacct ctcattatga ttcattgaac tttaccatat
      601 tgactggaat tttgatcaaa ttaacaaatc aaccgtatga aaaattattg accgatgata
      661 tcatcgaacc tttaggatta cagcatacaa aattttatga ccaagtcaaa aattcatcca
      721 atcatgcact atcctatcaa atgtctaaag aggatgatta ctttaaacca ctgacagaat
      781 ctgaaacaga gatccataac gaattaggga ctggtaatat cagtatgtcc gtctatgatt
      841 taaatacgct gtttacacaa gttttatctg gaaaactagt ttccaaagac ttactcttct
      901 cattatggcg tgaaaatgcg aatggacatc catatagtgg tggggtatat agcggagata
      961 attatatcct gtcgcaagga aatatcgact cttttgatac atctgctata tttagcaaag
     1021 attcacagga tgctatcatc atggaaagca atataaaagc tgacaaaact atcaaactgc
     1081 ctactgctga tctaagaaac caaatctatg acctgatgga aggaactact aagttggcaa
     1141 atagctgata tgatgaaaag atagcaaata ctacttcttt gaatagcatt tgcttaaaaa
     1201 aagtactcga ctaaacgtag gaaagagtgt gggacaaatc taaaaatcaa tttttgctac
     1261 agactttaat tccaaataaa cagaacagaa agaggtgttc ttctttctgt tctgtccaaa
     1321 ttattctaag atttctcttt accaacatta tttgacaaag ggcctattaa atctaaaata
     1381 gtatctttag attgctaaaa tatgtgctag caaatcattc atggcttgtt cttattttta
     1441 attttatgca ataaaaaaga agaacaatta gcgtgattat agaaataaat aatgagattt
     1501 tagaaaaaaa tgtgtatgaa tacctgattg ttactttatt tattccactt tcacttttta
     1561 ctgtaacaaa aggacctcct tgttttagta ctgtagcatt ttttgaatta acagtaaccg
     1621 ttaatccttt ataccggaat acaggtaaaa ctattgtttt gccttttgaa gatttaaacg
     1681 taaatgtaat ttcatcttta tttggagaaa agctactata aataacttga ccatcttctg
     1741 taacttgatg gtttttcaaa agcaattgat cttttttata gcctataggt acataatcaa
     1801 aattctcaaa ttcttcaag
//
LOCUS       DAZUKR010000230         1813 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000230 DAZUKR010000000
VERSION     DAZUKR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.235"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(268..702)
                     /locus_tag="ACG89C_002822"
     CDS             complement(268..702)
                     /locus_tag="ACG89C_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901297.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(719..>1813)
                     /locus_tag="ACG89C_002823"
     CDS             complement(719..>1813)
                     /locus_tag="ACG89C_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS4901298.1"
                     /translation="TTYFFYDYKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYL
                     FGPFRANNIDKVFFKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFT
                     GKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIR
                     NLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALI
                     QENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKE
                     IANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDS
                     SHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          633 a          296 c          205 g          679 t
ORIGIN      
        1 tgagactgtg acataagtaa aaaggtgact tcaaattcct tcttcggaat ttgaaatcac
       61 cttctttata attataaagt atcggtttca cagcatttct gaatgattcc tagaatctta
      121 aacgaatgat tggagttatc aacatttttt cactcgtttt tagcgaaaca aaaaatgaca
      181 tttgtcacag cctcattaaa ttattgattc tttttcttta taattgaatt gtccatattt
      241 aaagtgtcta gtactctaca tgaataatta attgctttca ctcaaatact tataatctat
      301 taacttgtag ttgtttaact tttctgaaaa agttagaccc acgtaataat cttcccagct
      361 ttctctttca attaactgat ccaatttttg ataatccata aattcatcat gtatcaaaaa
      421 gaataaataa cttgatattt tagcatctag cttaatcaga ttaaataatg tttgtttaaa
      481 attgctacca ttaatatgac tgatagattc tctagcttct tcacataaac tttttacttt
      541 attaattact actttgtcat attcatttaa cacactatta ttaccattac ttagacgctc
      601 agattctacg aaacaactca aatcagaaat ttcccaaaaa aattttttgt taaaatccaa
      661 tttcctcaac tcctccaaac tacgtaaatg atatagtctc ataataaaat aaaacctctc
      721 agctaatgtt gttgaaattg tccactaaac ctaattgata ttgcttagga gatacacctg
      781 cgtacttttt gaatactctc gaaaagtgtg aagaatctga aaatcctaaa cctaaagaaa
      841 cttcactgat agtatggttt gatttcaaca ttattttagc cgtagaaatt tttcgcttag
      901 agatataatt ataaatcgta atatgcattt cattcttaaa gctttctctt aatttagttt
      961 cacttacaga aaaataatta gctatttcct ttacagtaat tcttttatag atatttaagc
     1021 cgatgaattg aacgacagaa gaaattaagg gagaaagatg ctctatctta gtttttccga
     1081 tttccttagt ataataaaca atcccagaat ccctcacttt taagacctca ggtaacgata
     1141 cagctaattc gttttcttgt atcaaagcat ctctactttg atacgcttct attatatcca
     1201 ttcctaatgt tatagacgtt tgtgctaact tttcaaaaac aatgatagaa taattttttt
     1261 cagaccgaat cgtatctcca ctaacacttg gaattattcc attactcaga ttaaaaacca
     1321 tgttttctaa atttcgaata tctccatttt taacatattc cagtatttta ttttcataaa
     1381 gaaacagata tatttctgga tcgtaatttt gtgataacag attatctatt ttgatttgct
     1441 cgatagtttt acttagattt tctgtatatt caattaacgg ttcatggaat agatcttcta
     1501 tttttccagt aaaaaaataa tgaactaaaa tcagaatatc tcgaatgtct cctaaagaat
     1561 acaaagtcaa attttgcaat gaattataga gtcgttctct atcagccatt gcaacattca
     1621 aattgttcat ttttagttta aaaaaaactt tatcaatatt atttgctcta aatggaccaa
     1681 ataagtaata tctggtttta aacatgtata gaagaaacat ctcattaaaa tcaccagtaa
     1741 agaaatggaa atctttagtt tttttaatat tatcagttag taacttttta taatcataaa
     1801 aaaaataagt agt
//
LOCUS       DAZUKR010000231         1794 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000231 DAZUKR010000000
VERSION     DAZUKR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.236"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..671
                     /locus_tag="ACG89C_002824"
     CDS             153..671
                     /locus_tag="ACG89C_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901299.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            694..1719
                     /locus_tag="ACG89C_002825"
     CDS             694..1719
                     /locus_tag="ACG89C_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4901300.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
BASE COUNT          616 a          205 c          383 g          590 t
ORIGIN      
        1 agtccgtatt taaatatcat gcttaacaat ctggaaaaac ttcagttgca aaaaataagt
       61 atgttattgt ttataggtgt atgctgttat cgtatgttat ttaaaacatc tttgtttggg
      121 gattgtgctg ttttaattgc actatatgtc atatgttata cactgaaaaa aacgataata
      181 atatttttga gagacacaga atcataggaa caattgtatg tataacagtt ataccacttc
      241 tcatatccat aggaatactt ttcaaattta taccaaatgc aatggttaac cagatggttc
      301 atattggttc tccattgatg gttatagcaa gtatattttt attttatgta tttaaaaatt
      361 tttcatttta cagtaagtat ataaataaac ttgcgagaac cagtttaggg gtatatattt
      421 tacatgaaaa tcattatttc attgatggta tgaagtctag aggcttaata tgggatggga
      481 tatttggatt aggcaaaagg tttttagata gggatttttg gatttggttt ataattgcag
      541 ttgtagttgt gtgcataatt tgcatggcta tcgatgttgt aagagaaaaa tttgaaagac
      601 agatttttaa aattaagagt gtaaagaacc tgtgtaaact tatgaatcat gttataaata
      661 tatacccata attaataaat gaggagaaaa gaagtgaaag ttatatcatt cggagaatta
      721 ttattgagac ttgcagcacc cggttattca aagttatttc aaagagatag tttggagact
      781 acattctgcg gaggggaagc taatgtagcg gtatccttag caatatttgg atatcaatca
      841 gaatttataa caaagcttcc ggattcagat gttggatatg cggcagctaa tgctttgcgt
      901 tattttggag taaatgttga taatattata tttggagagg gacgcatggg actgtattat
      961 ttagaaaaag gagcagctca gagaccatct aaagtaattt atgacagaaa attttctgca
     1021 atttcgttag ctgatagtga tgaatttaag tggaaggaga tcctaaacga tgcaaattgg
     1081 tttcattgga cagggataaa tccagcattg tctgagaatc tctttgagac gtccttagat
     1141 gcatgtcagg ctgccaaaaa aagaggtata acaatttctt gtgatttaaa ttatcgatcc
     1201 aagctttgga catcagagga agcaaaagaa aagatggaac agatactacc attggttgat
     1261 atttgtattt gtaatgaaga agatgcagaa aaagtgctag gaattaaagc tgagcataca
     1321 aatattaata gtggagaatt aaataaagat ggctatgtgc aggtggctag ggagattcac
     1381 aagaagtttg gatgtaggta tgtagctaca actctgcgta gaagttattc ggcaagtaga
     1441 aatggttgga gtgcaatgtt atacgaagca gaaaaagata aagcgtattt ttccacagaa
     1501 tacaacatac aaattgttga tcgcgttggt ggtggagaca gcttttctgc tggtataata
     1561 tatgcgttat gtgaaaatat gtccggacaa aaggctattg aatttgcgac tgcatctagt
     1621 tgtttaaaac atactataga aggtgatttt aatcgaacta caattgagga tgtatataat
     1681 ttaatgaata gcggtggaaa tggtcgcgtg ttgcgttagt caacatataa agataaatca
     1741 cagtaatttt tatggtgatt atctgtctta gtagtgatta gaataacgtg aaaa
//
LOCUS       DAZUKR010000232         1744 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000232 DAZUKR010000000
VERSION     DAZUKR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.238"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1382
                     /gene="brnQ"
                     /locus_tag="ACG89C_002826"
     CDS             30..1382
                     /gene="brnQ"
                     /locus_tag="ACG89C_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4901301.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          479 a          307 c          348 g          610 t
ORIGIN      
        1 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
       61 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
      121 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
      181 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
      241 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
      301 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
      361 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
      421 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
      481 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
      541 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
      601 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
      661 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
      721 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
      781 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
      841 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
      901 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
      961 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
     1021 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
     1081 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
     1141 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
     1201 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
     1261 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
     1321 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
     1381 gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa
     1441 tggcttctca catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc
     1501 gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa
     1561 aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca
     1621 ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc
     1681 tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat
     1741 ttct
//
LOCUS       DAZUKR010000233         1738 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000233 DAZUKR010000000
VERSION     DAZUKR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.239"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="ACG89C_002827"
                     /pseudo
     CDS             1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="ACG89C_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1218..1610
                     /locus_tag="ACG89C_002828"
     CDS             1218..1610
                     /locus_tag="ACG89C_002828"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4901302.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          715 a          133 c          297 g          593 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttattgatga taatatggtt tttgatattg
     1261 ataatttaaa aggatttctt aatgatacca gttcatttgg gtttatagct aaagaaaata
     1321 ataaaattat aggatttgca tattgctata cacttttaag acctgatgga aaaacaatgt
     1381 tttatttaca ctcaatagga atgttaccta actatcaaga caaaggttat ggttcaaaat
     1441 tattatcttt tattaaggaa tattctaaag agattggttg ttctgaaatg tttttaataa
     1501 ctgataaagg taatcctaga gcttgccatg tatatgaaaa attaggtggt aaaaatgatt
     1561 ataaagatga aatagtatat gtatatgatt atgaaaaagg tgataaataa atgaatatag
     1621 ttgaaaatga aatatgtata agaactttaa tagatgatga ttttcctttg atgttaaaat
     1681 ggttaactga tgaaagagta ttagaatttt atgatggtag agataaaaaa tatacatt
//
LOCUS       DAZUKR010000234         1736 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000234 DAZUKR010000000
VERSION     DAZUKR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.240"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG89C_002829"
     CDS             complement(<1..182)
                     /locus_tag="ACG89C_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4901303.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG89C_002830"
     CDS             517..951
                     /locus_tag="ACG89C_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4901304.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG89C_002831"
     CDS             974..1294
                     /locus_tag="ACG89C_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901305.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG89C_002832"
     CDS             1435..1626
                     /locus_tag="ACG89C_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901306.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          659 a          260 c          363 g          454 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataata
//
LOCUS       DAZUKR010000235         1664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000235 DAZUKR010000000
VERSION     DAZUKR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.242"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG89C_002833"
     CDS             131..505
                     /locus_tag="ACG89C_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4901307.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG89C_002834"
     CDS             complement(619..1248)
                     /locus_tag="ACG89C_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4901308.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG89C_002835"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG89C_002835"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZUKR010000236         1657 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000236 DAZUKR010000000
VERSION     DAZUKR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.243"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..510
                     /locus_tag="ACG89C_002836"
     CDS             <1..510
                     /locus_tag="ACG89C_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901309.1"
                     /translation="KNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSI
                     FSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPS
                     MMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAEL
                     RKLVEYGII"
     gene            497..>1657
                     /locus_tag="ACG89C_002837"
     CDS             497..>1657
                     /locus_tag="ACG89C_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4901310.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIA"
BASE COUNT          538 a          215 c          267 g          637 t
ORIGIN      
        1 aaaaacatac aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt
       61 tttgatcaac ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa
      121 gtaaatagta tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg
      181 acaaatcgtt atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta
      241 cttgaaaatg acttctcttc aacacttttg attagtccta tatctacaat ttctttttca
      301 ccatctatga tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat
      361 gaatatacag cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt
      421 gaacattata accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta
      481 agaaagttgg tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc
      541 cagtttctgg tatactatag ggaatttttt tattaaggga atttcattca taactattcc
      601 tatttttgta aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt
      661 tgtatcaatt ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag
      721 ttttgactat aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact
      781 ttcgttaatt gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt
      841 tgatatgaac caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat
      901 tagttatatc cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc
      961 ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt
     1021 tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt
     1081 agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag
     1141 atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc
     1201 acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc
     1261 ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg
     1321 ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag
     1381 taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga
     1441 tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat
     1501 aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg aattttatga
     1561 gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt
     1621 aaacatgatt ttcataccta ggttaggaat aattgct
//
LOCUS       DAZUKR010000237         1644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000237 DAZUKR010000000
VERSION     DAZUKR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.244"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..338)
                     /locus_tag="ACG89C_002838"
     CDS             complement(<1..338)
                     /locus_tag="ACG89C_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4901311.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELIS"
     gene            complement(472..>1644)
                     /locus_tag="ACG89C_002839"
     CDS             complement(472..>1644)
                     /locus_tag="ACG89C_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFS4901312.1"
                     /translation="EILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDYEKYDRI
                     GFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLF
                     RKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAIYMDATY
                     IPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERGLKNVLL
                     FITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSK
                     EVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIESFNKQI
                     KKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQLIEN"
BASE COUNT          470 a          341 c          306 g          527 t
ORIGIN      
        1 gaaatcaatt cttctaagat tgtttgactt tcagaattta aagaaatgga agcacaaatc
       61 tcattgtcaa attcttcttc gctgattcct tcaaatgtcg cagagataat aatccaatgc
      121 ccttgccacc cattttgaat tccatgaaaa aaatctgatt cttttagtgt tttactacta
      181 tagtaataac tatcatctaa taagattctc aatgcagtaa gagcattggt tttccctgaa
      241 tcattctcac ctataatagt atttgtctca ttatcgaatc gaaagatttc ggattcataa
      301 tttctaaaat ttcgaatttg aagagtagat ataaacataa gcagatctcc taactattta
      361 attaatatga ttatatagta agtttatcat aaattatgtt agtaaaagtt ctcattataa
      421 gggagcgtca ataattttgt gtaaatgatt cttccctact gcagattgtt tctaattctc
      481 aatcagttgg cttagcattt gttcgagctc gccttcggcc tgttggaaac ctttatgact
      541 gcgacctagg aatttttggt tgtaagtatc aaaagacgag actaagaaac gttccatcga
      601 ctcttcattt tggaactgtt ccttgcggtg gctgtatttc ttgatttgtt tattaaagga
      661 ttcaatcaag ttcgtagaat aaatactctt gcgtatggcc aaggggaaat catagaaagt
      721 gagcaagtga tcgttcgaaa gaatcgattc aaccactttt ggatagctgg ttttccattt
      781 ttccgcaaaa gtaccacgtg cttccaacgc cacctcttta gatgaggctt gatacaccat
      841 tttaaaatca tcacagactt ccttacgatc atcgacacgc actttatgac tgatattacg
      901 ggaaacgtgt acacaacaat gttgaaaacg agctttggga tagaaccgac taatcgctcc
      961 taccatcccc tttaagccat ccgtgatgaa caggaggaca tttttcaaac cgcgctcttg
     1021 aaggtccaat aaaatttcct cccaaatcgt gatggattca gtcggtgcaa tcgcatagct
     1081 caatacttcc tttgatccgt ccgggcgaat gccaactgcg atatgaatag cttccttggc
     1141 gacggttttc cgctttaacg gaatatacgt tgcgtccata taaatagcag cataacggtc
     1201 atgaagctcc cgcccattaa acgccgttac ctcttctgta aatgattttg ttatattgga
     1261 catggtttgg ggcgtgtagt aatgcccata cattttctca atcaagtctg cgatttccga
     1321 catggtaata ccttttcgga agaggtgaat gacggtctcc tctaacgtgt cattcgtccg
     1381 tctataagca ggaacagtct gttgcttgaa ctcaccattg cggtcgcgcg gaatctggag
     1441 atgaagttcc ccatactcgg tcttgaccgt acggtcatag gagccgttac gagaattacc
     1501 cgtgttaaaa ccaatgcgat catacttttc gtaatctagg aaagccgtta actccgtttt
     1561 gagaagcgta ttgactgctt tctccaaatg gacacggaat aattcattca aatcgccttt
     1621 gttcgctaga gtctttagaa tttc
//
LOCUS       DAZUKR010000238         1638 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000238 DAZUKR010000000
VERSION     DAZUKR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.245"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..400
                     /locus_tag="ACG89C_002840"
     CDS             2..400
                     /locus_tag="ACG89C_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4901313.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="ACG89C_002841"
     CDS             384..1079
                     /locus_tag="ACG89C_002841"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4901314.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          617 a          238 c          325 g          458 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgcca
//
LOCUS       DAZUKR010000239         1612 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000239 DAZUKR010000000
VERSION     DAZUKR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.246"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..338)
                     /locus_tag="ACG89C_002842"
     CDS             complement(108..338)
                     /locus_tag="ACG89C_002842"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS4901315.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(432..770)
                     /locus_tag="ACG89C_002843"
     CDS             complement(432..770)
                     /locus_tag="ACG89C_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901316.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          539 a          284 c          208 g          581 t
ORIGIN      
        1 actctggata ctctttatac tgcattaaag ttagtaatat taacagcaaa gatatcgaaa
       61 aaacaataac ataatatgtg tcaaaatttt tcataataag aactccctta ctgttgcata
      121 ctaacataat ccaaaaactc ttctacttca gctaaagaca ttccactttc tttagtccaa
      181 aaaacagttt tttctatcga tccttctctg taaactgtaa atgtcggagt cgagttaatt
      241 gagtagcgac tcttgaattc tttccactca ttttcatctt ctctaacttt tgctacattc
      301 acgtaataaa tattttcttt tcccttttct accagcatat cgaatagctt cctgcttttg
      361 atgatcgatt tcttgtgcta catctagtaa attttcatca atttctatat cataaagtat
      421 ttcactagtg tctacatcca caatatatac tttttatcag tagcaatttt tgagtaatcc
      481 gttaaaatga tctcagtctc gtcactttca tcagtataaa tatacgttaa caatgaactc
      541 accttttcaa tcggtgttag ctctaagcct actgtatttt ttactattga gtcattattt
      601 accaccataa cttcgtactg tatataccat cctatttggt ttccatcaat atcagtaatt
      661 ggctcactag ctaatggagt ctccggaaca atattttcct gatatttctt aaactcttct
      721 gtagcacgaa aaaggttatc atgttcctca cttgaaactg gttgagtcat agaaaatgtt
      781 acttggctaa acagagggaa aataaacact acaatcaaca aagtataaaa aaaagccctt
      841 ctttttcatt ttttatcttc caatcacaaa ttgaaaaact tattcctgca actttgagtc
      901 tagtatcaca atcgactgac tacaactatt catttacatt ctactttaag ttcctcctta
      961 accattaatt ttattaaaga tacttgtaat agtgattctc acaaaatttc atgaaaaata
     1021 ctttattact gtatctaaaa ttccaataca caacttatcg actattatct gaaaaccaaa
     1081 acttattatt ccttgatgtt attctagcaa tagtaactgc ttttttattt ttttgcatat
     1141 tttttcggcg ttacttttta aagttaactt tttcgacaaa tagaaagaga tacgaatcac
     1201 tgattttttt gaagaaaaga aatggagtga gctgaacgtt agagatattg ggagctttcg
     1261 taactgctta ttaaacgata atggttattt gcaaccgtat tgctcgcttc tacatgaaat
     1321 atttcatgaa atatttcgca aaacctttga ttatgtagtt acgtttccat tcttgactaa
     1381 aaatgtttca aaaacgaaca aaggtgttaa aaggtaaagt gatcaatact actggtcaaa
     1441 ataagaggtt gaaagggaat tgaaaaataa ctacagataa tacttttata aacgtacgtg
     1501 ctttgtggtt ctgtagttaa ctatataact ggaattaggg aatggtatca atttgaaaag
     1561 aaagattcta ccaatttatc atatgttaaa aatggaaaat caacagcagt tc
//
LOCUS       DAZUKR010000240         1587 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000240 DAZUKR010000000
VERSION     DAZUKR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.247"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..1543
                     /locus_tag="ACG89C_002844"
     CDS             77..1543
                     /locus_tag="ACG89C_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS4901317.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          442 a          291 c          332 g          522 t
ORIGIN      
        1 aataagtttt ttagttaaat ttttcggtaa agatgtataa tattttctga aaatttaaag
       61 aattggggta tagattatgg aaaaacagca agacaagggc ttcttcgggc agccgaaggg
      121 attgtcaact ttgttcttca ctgagatgtg ggaacgattt agttattacg gtatgcgtgc
      181 attacttatc tattatatgt acgatacagt tgccaacggg ggacttggat taccacgagc
      241 aacagcatta gctatcatgt cgatctacgg atctttgatc tacatgtcta gtatcgtcgg
      301 cggatgggta tctgaccgag tgttaggagc aaaacgcact gtattcttcg gtggagtttt
      361 cattatgctt ggccacatcg tcttggcttt accatttggt gtaagtacat tgtttgtttc
      421 gatggcattg attattatcg gaaccgcctt tttgaaacca aatgtttctg gcatggtcgg
      481 acatctttat tcaaagactg acttgcgaag agatgcagga ttttcgattt tttacatggg
      541 tgtcaactta ggggcattat tagcaccact aatcgttggt acaatcggtc aagaatataa
      601 ctatcattta gggttctctt tagctgctat tgggatgttc tttggattat tacaatacat
      661 catccaagga cgcaagacat tagacggagt gggtacaaca ccgccgaatc ctttgacaac
      721 tgaagaacgt aaaggattca tccgtaactt gatcatcgca cttgttgtta tcgctgcagt
      781 attcggcggg gcacaactaa ctggtcatct gacaattgac ttttttgtca atacaatcag
      841 tgtattgggg attatcttac cgttgtatta ctttatcaaa atgctgactt caaaagatgt
      901 aactgctgaa gaaaaaccaa gagtatgggc ttatatccca ttattccttg cagcaatcat
      961 tttctggtca ttacaagaac aaggttcatc tatcttagct ttatttgcta gtgaacgtac
     1021 acaatctacg ctatttggtg taacgatcca agcaagctgg tatcaatcat tgaatccaat
     1081 tttcatcttt attctgacac cagtattcgt aacgatgtgg acgaaacttg gtgaacgtca
     1141 accatctaca gtagtgaaat tctctcttgg acttttattt gcaggattat cgtacttgct
     1201 attgatgttg ccgggtatgc tttatggtac aagtggaaaa gtaagtccat tgtggttgtt
     1261 gggaagtttc ttcatcgtgg ttattgctga attgtgtctt tcaccagttg gtttgtctgt
     1321 gacaacaaaa ttagcaccac gagcatttga atcacaaaca atcgctattt ggttcttagc
     1381 tgatgcttct tcacaagcaa tcaatgcaca aattgcacgc ttctacacac cgggaacaga
     1441 atctgcttac ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct tgctatttac
     1501 tgtgaaagaa ccaatcaaaa aactaatggg ctctattcat taatcaatga acataaaaac
     1561 aggaattgct tatttgcagc agttcct
//
LOCUS       DAZUKR010000241         1576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000241 DAZUKR010000000
VERSION     DAZUKR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.248"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..792
                     /locus_tag="ACG89C_002845"
     CDS             76..792
                     /locus_tag="ACG89C_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4901318.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            811..>1576
                     /locus_tag="ACG89C_002846"
     CDS             811..>1576
                     /locus_tag="ACG89C_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFS4901319.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKP"
BASE COUNT          609 a          191 c          302 g          474 t
ORIGIN      
        1 cattatttca atgattaaga gtcgagagca caaacggtag acgatgttat ttttataaaa
       61 agatataatt tattaatggt cgataattat gaaaggaaaa atattcttaa aaaaaagagt
      121 ataggatgtc gtatgaaaat agtcgctata gtaccaatga agttaaataa caaaagattg
      181 ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat tgtgttatta tatactttca
      241 acacttttaa aagtaaaaca gattgataaa gtgtatgtct attgtagtaa cccagatatt
      301 aaagaattta taccagaagg tgtaacattt ttaaagagat ctagttcctt agatcaggat
      361 acaacgagta ttacacaggt attagccggt ttcacaaaag aagtagaggc tgatgtatat
      421 gtaatgacac acacaacagc accatttatt agcgaaaaat ctattgaaaa aggactagat
      481 gccgtgctga caggagaata tgattctgca tttgctgcaa aaaaattgca agatttctta
      541 tggaaagacg gtgaaccatt taattatgaa ttggacaata ttcctagaac acaggatttg
      601 ccgcctttat acgaagaaac tagtggattt tatatttata atcgcaatgt gatgaccgag
      661 ttgcatagaa gaattgggaa aaaaccttgc atagttgaag taggtgagat tgagtcagtt
      721 gatattgatg aagccgagga ttttatgatt gctgatgcga tttataatca tcttatttta
      781 caaggtagtt aagacagggg gaaggttagg atgaacaatg tacagatttt agattgcaca
      841 cttagagatg ggggctattg taacaattgg gaatttggat ttgataatac aaaaaaaatt
      901 atttctggac ttatagaatc aggaattgag attatagaat gtgggtttct cacaaataaa
      961 acagtataca ataaaaatgt tacaaaatat acgaccttag aagaattagc acagataatt
     1021 cctgaaaaaa gagaaggaaa attatttgta gcaatgatga actatggcga atataatata
     1081 gaggatcttc cagattatga tggttcatct attgatggaa ttaggattgc gtttcataaa
     1141 aaaaattgca atgaagcgtt gaaattatgt cgtgagataa agaaaaaagg ctacttagtt
     1201 tttgttcaag ctatggtatc tctcaattat acagatgaag agtttttagc agtaataaaa
     1261 aatgtgaatc agtttaaacc atacgctttt tatattgttg atagttttgg aatgatgaaa
     1321 ggaaaagatt taactcgtct tttttacatg attgagcata atctagaaaa agatatacag
     1381 ataggttttc actcacataa taacatgcag cttgcatatt ctaatgcaca aaaacttata
     1441 accgtacaaa caaatcgaag cctgattatt gattcttcgg tgtacggaat gggaagagga
     1501 gcaggtaatc ttaacacaga actatttgta gaatatttga atgataatgc aggaaaaaaa
     1561 tatcaattaa aaccat
//
LOCUS       DAZUKR010000242         1576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000242 DAZUKR010000000
VERSION     DAZUKR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.249"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG89C_002847"
     CDS             <1..963
                     /locus_tag="ACG89C_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4901320.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ACG89C_002848"
     tRNA            1152..1225
                     /locus_tag="ACG89C_002848"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
BASE COUNT          528 a          283 c          304 g          461 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1441 taataaagaa gagtttaaag cgttccccaa aaaccactgc cccataagtg tgttacgctt
     1501 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     1561 aaaaaggctt ataatt
//
LOCUS       DAZUKR010000243         1572 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000243 DAZUKR010000000
VERSION     DAZUKR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.250"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..831
                     /locus_tag="ACG89C_002849"
     CDS             70..831
                     /locus_tag="ACG89C_002849"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4901321.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(946..1386)
                     /gene="fabZ"
                     /locus_tag="ACG89C_002850"
     CDS             complement(946..1386)
                     /gene="fabZ"
                     /locus_tag="ACG89C_002850"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4901322.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          531 a          290 c          278 g          473 t
ORIGIN      
        1 aagtaggtat tttccctcta ttttgttatc attaaagata acaaaatagt aaattggaaa
       61 ggaacagtta tgaagaaaaa taaagttatt ttaagtatta gtttagcagc agcattgctg
      121 tttgctgctg gttgttctgc aaacaacacg acttcttcta gttcgtctac agaaagttca
      181 aaaagtacat ctactgcgga aagcacgact gccagtagcg tcgatttgaa cgcattggaa
      241 ttaccacaat tgacgaaaga tgtcgccgaa aatgaagatc tggtcgaaat ggtgacgaat
      301 aaaggcacaa tcaaaatcaa attatttccc gaacacgcac caaaagcggt tgaaaacttt
      361 atgacacatg caaaagacgg ttactacaac ggactgactt tccatcgcgt catcaaagat
      421 ttcatgatcc aaggtggcga tcctaaaggc gatggcactg gaggggaaag catttgggga
      481 gaaggatttg aaacagagat caacaaccac ctttataata taagaggtgc gttatctatg
      541 gctcgctcac aagatcctaa cagtaacggc agccagttct ttatcgttca aaatacagat
      601 gatatgcatg atggtctttt aaaagacgac tatcctcaag caatcatcga cgcttataaa
      661 aacggcggtt atccttcact agatggccat tatactgtct tcggccaagt gattgaagga
      721 atggatgtcg tggatgctat tgcaaatatc gaaacagata gttcagacaa accaaaagaa
      781 gaagtaaaaa tcgataaaat caatatactg caagaagcaa aacaaaaata aaaaaataac
      841 gtctgaatcc tgcatagatg acaacgtatg ctaggattca ggcgtcttct ttttacataa
      901 aaagcaagta agcttccttt tgttttgcta tatcaggttt tatagtcaag cattttggtt
      961 gacgatgaac gtgaactcgc actcacagac ttttttatca tctacatatg ctgcagataa
     1021 cgctgtaccg actgatccct tcattttggt gatttcaaaa tgacatttca gcacatctcc
     1081 tggaacaact ttttgacgga atttggcctt attgatccca ccgatataag ctctttttcc
     1141 tagaaactct tctgatttta ggataagtat cgaacctgct tgtgccagtg tttctagaat
     1201 caacgcaccc ggcatcactg gattgtcagg aaagtgacct tggaaaaaat cttcattgat
     1261 tgttacattt tttgtcgcaa cgatcttttt tcctggttct aaagtatcta cataatcaat
     1321 ataacaaata ggataacgat taggaatcaa ttccattact tcagtagcag ataatacgta
     1381 agacataatt aaactccttc tttcaggcaa ttgccatttt cattactttg actatcaaag
     1441 tatcatattc ctaaatattt agcaatcttt ttttaattta agaaaaaata gtttgacaat
     1501 caaaacattt tgactaataa ttaagaaatc aagaagttat tttagctatt attctctatt
     1561 tttttacgac ta
//
LOCUS       DAZUKR010000244         1570 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000244 DAZUKR010000000
VERSION     DAZUKR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.251"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          592 a          179 c          320 g          479 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg
//
LOCUS       DAZUKR010000245         1551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000245 DAZUKR010000000
VERSION     DAZUKR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.252"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG89C_002851"
     CDS             132..344
                     /locus_tag="ACG89C_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901323.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACG89C_002852"
     CDS             506..1024
                     /locus_tag="ACG89C_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS4901324.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACG89C_002853"
     CDS             1031..1543
                     /locus_tag="ACG89C_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901325.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DAZUKR010000246         1551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000246 DAZUKR010000000
VERSION     DAZUKR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.253"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1509)
                     /locus_tag="ACG89C_002854"
     CDS             complement(28..1509)
                     /locus_tag="ACG89C_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901326.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          492 a          212 c          271 g          576 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca a
//
LOCUS       DAZUKR010000247         1526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000247 DAZUKR010000000
VERSION     DAZUKR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.254"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..368)
                     /locus_tag="ACG89C_002855"
     CDS             complement(<1..368)
                     /locus_tag="ACG89C_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4901327.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKT"
     gene            complement(365..1018)
                     /locus_tag="ACG89C_002856"
     CDS             complement(365..1018)
                     /locus_tag="ACG89C_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4901328.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1306..>1526
                     /locus_tag="ACG89C_002857"
     CDS             1306..>1526
                     /locus_tag="ACG89C_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4901329.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEK"
BASE COUNT          487 a          261 c          163 g          615 t
ORIGIN      
        1 tttgtttttt taccatcatt aagctttatt tctctttctt caattatcaa agaattacta
       61 tcactttcta aattaacatt tatattatct ttgatttgta tttcattctt attatttttt
      121 tcttttatga atacttttat ttcactactc ttggaataaa actccaaagt ttgttctata
      181 tattttattt cttttccatt catattacta gaaatttcat ttgccatatt atgaatcttt
      241 tcttttctcg tctccaaata tgttttagga aagataaaaa aaattaatga atgaactaat
      301 ataattatta ttccaagaac agaaaatatt tgtatgaaca tttttggaaa tatctttaat
      361 tttttcattt tctctccaat ttatatccta catttcttat agtagttata cagtctaatt
      421 gtaatttttt tctaagttct tttatatata catctattac tcgatcataa ggagtttctt
      481 cgctatcttt ccatacataa tcaataattt gcattcttgt taacacttgt ccattattat
      541 ccaataaaca ttttaatact tctagttctt ttgcatttac atctatttct tcaccattta
      601 ttttagctgt atagctatta aaattaaccg atagatcctt atattcaaac ttctctaaat
      661 gtccataatt cttcttaatt aaagaatcta ttctagcttt taagaccggc aatgaaaatg
      721 gcttttctac atatccgtct actaaattag taaatgcatc aattttatat tcttcatcac
      781 taaatgcagt caagattaga attggtaaat tgcttttctt tctaatttct ttcaacactt
      841 ctaaaccatt tataaaaggt atctgaatgt ctaagataac caaatttata tcgctattaa
      901 attttgacaa ggcttctctt ccatctttag cttgaataac agtatatcca aattctgaaa
      961 gatattcact tattccctct cttatcatat tatcatcttc aacagttaat attttcatat
     1021 atacctccac ttaagcctat acactagata ttatacagca tattatggct ctataatatc
     1081 tagtgttggt gacaccttct gttgccaata ctttttcttt tttctaataa atgcaaacag
     1141 acaccttgaa atcctttaga ataaagggtt ctataatatt ttgtgttgtt ggaaaatcga
     1201 aggcgatttt ctactaacac tttttcttcg tttcaactca aaaagagact cacttttggt
     1261 aaaatttagt cgaccaaaac atcaccctac caaaggagtc gctctatgga taatcatact
     1321 agaaaattac tcggattaac agataaacat ttattttttg atgaagaatg gctgattgaa
     1381 aaagaatata aaggcgttca agcgcagttt attaaaggga gattggatga ctcaccttcc
     1441 cattgtgaac actgtggatc gattcgtatt atccgcaacg gttcctatac gacacgtact
     1501 cagatattga aagtcaaaga aaagtt
//
LOCUS       DAZUKR010000248         1509 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000248 DAZUKR010000000
VERSION     DAZUKR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.255"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..868
                     /locus_tag="ACG89C_002858"
     CDS             101..868
                     /locus_tag="ACG89C_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4901330.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ACG89C_002859"
     CDS             1060..1458
                     /locus_tag="ACG89C_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4901331.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAZUKR010000249         1479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000249 DAZUKR010000000
VERSION     DAZUKR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.257"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACG89C_002860"
     CDS             191..445
                     /locus_tag="ACG89C_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4901332.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACG89C_002861"
     CDS             446..763
                     /locus_tag="ACG89C_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4901333.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          527 a          217 c          283 g          452 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgc
//
LOCUS       DAZUKR010000250         1469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000250 DAZUKR010000000
VERSION     DAZUKR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.258"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..392)
                     /locus_tag="ACG89C_002862"
     CDS             complement(93..392)
                     /locus_tag="ACG89C_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4901334.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            743..1375
                     /locus_tag="ACG89C_002863"
     CDS             743..1375
                     /locus_tag="ACG89C_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4901335.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          502 a          237 c          202 g          528 t
ORIGIN      
        1 aaaaaaatac agactctgtt caaataaatg aacctagcct gcattatgag attgactata
       61 ctttttaatt cattgagatg aataactcgt ttttataaat gcccaaaagt aatcggtgcc
      121 gaagccattc ctcgacctaa ttctttttca aattttccta ttgttgatat ctccatataa
      181 aacggtgata ctggtttaga caattctccc ctgattgcta acggacgtaa actacgttcc
      241 aaattattta tgactttagg ataatactga cctttttcta agcctgtctt tgcgtctatc
      301 aaaagtatac gttccttttc tttgatctct ggatttaaaa cgagatcata taattttagt
      361 accatgtttt ctatttcttc acttttagac atcacgattt cctcccaaaa atatatttct
      421 tcccactaca ttctataaac aaagtatacg ctacatctag atttttttaa atcattactg
      481 gattgttaaa acctttcaat acactgttat ggcaacgatt tttattttaa aaacattcta
      541 aattaaatca gtaaaaattt ttattctaag atttttatta gaattacgcc taatttgtgt
      601 ctctcagggc aattctaaga acgtttcaag agatagcgac actatcgtta tcgaaaaagg
      661 cgaaaaccta tctcattttc tgctaatgta agttggttta gcttttgtta atatgacttt
      721 atgaaaatgg aggtaattaa atatgatttt tcctaaagtt aatgaagcaa gcttgaataa
      781 aggatccttt tttcatatct atgaaaacga agattttgaa ctgtataata tcctttcaca
      841 aaaagaggtc atcgaaaacc aaataatctg ccatccaatt atttacaaat ctattttcaa
      901 gtatgcattt tttgcttcca atgattttca aagaatggag attaccgact gtatattcga
      961 aaactgtgat ttttcaggct gtatattgac aaaaagcatg ttgcatcgcg tgaaatttgt
     1021 caattgtaaa ttaacaggta ctaaattgat ggaaagttat attgggaaca cgttattcga
     1081 gaattgtaaa atggactatg taaatttatc tggttcaaac ataaaagaaa gtacctttga
     1141 gcactctgtc ttatcaagcg cagattttgt agactgttcc ctcactaaaa taatgttttc
     1201 tactaatgat ttagaaaatg ctagcttttt tgatactgat ttaaaaaata tcgatttaag
     1261 tagcaactcc tttgaaatga ttgaagtacg ctcggaacat ctaaaaaatt gtacgcttaa
     1321 tcagttgcaa gcacttggtt ttgctagaaa atttttacaa ataaaagtag tataatcaaa
     1381 atgaatatat aagataaatt tttagcaata caaatatcca taaaaaaaca ttgcctattt
     1441 taactcttca tgtatactga ctctatctt
//
LOCUS       DAZUKR010000251         1454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000251 DAZUKR010000000
VERSION     DAZUKR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.259"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..427)
                     /locus_tag="ACG89C_002864"
     CDS             complement(<1..427)
                     /locus_tag="ACG89C_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4901336.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSV"
     gene            complement(428..1336)
                     /locus_tag="ACG89C_002865"
     CDS             complement(428..1336)
                     /locus_tag="ACG89C_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFS4901337.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(1336..>1454)
                     /locus_tag="ACG89C_002866"
     CDS             complement(1336..>1454)
                     /locus_tag="ACG89C_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4901338.1"
                     /translation="YLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          483 a          252 c          155 g          564 t
ORIGIN      
        1 caactgaaaa gtagacgtct ttatctaaat tttttagatg ttcatctatt aattcttcaa
       61 gattagaaac ttcacgtaaa ggtgaagtca aatctaaatc tataattaaa tcaaatatat
      121 tatcttctct ttctagttgt aagaatgaat cacgtatcac ttcaattttc cctacagtat
      181 ctgtagctaa ttcaggttta cgttctacaa ttttcacaga cggatttaac ttagtcgtca
      241 attctactaa ctcattactg tccgtattca aacaagtcgt gcaattatgt tcaggatgtt
      301 tttgttgata gatatctatc attgctaaag cgaaatggac taggggcttt cctgccaaat
      361 atttcaaatt tttattttta aaccccttag acccagctct accacaaata gtaaataaaa
      421 tttccattta tttctcctca gctattttca atacttcgtt tgcatgatat aaatcattcc
      481 agttttcttt gcctgttact acgttttcaa agaaatattt caactccttc tcatacatat
      541 cattagcaga ttcaggcatt tctttaacaa tgttttcttc aaaacttatt ttattattta
      601 ataagtcaac attgatacat tcatctaaag taaacaattg aaattttctt gtaggaactc
      661 ttccaaaata atctaaatga atttctataa ttttatcatc gtatctcaat atatatgttg
      721 ctaaatcatt actgttaatt tcaagattcg aatatttacc aaactgtttt tttactgatt
      781 tagggaaacc aaataggttt ataatataat ccaattcatg tatacagtct aattctactc
      841 ctccccccat agatttaaag gcagaataat tatttctata atcagtacct tttctccaat
      901 caggaagata agtagaacaa atagctctta cagaatatac ttttttactt tgaatactat
      961 tacgaacaac ttccatgata tttttatacc tcagtggagc agcaacataa atatttttgt
     1021 aatcataatt atattgagta agatcaatac tctcagaaaa agcaggtttt tctaaaaata
     1081 tgtaatcaac tttttcaaca attacattta gtgtttccag atgcttaaaa gtcggatttg
     1141 taataaaagc tatatcataa tgtgtatcca aatcgtctct attttgaata tacgttattt
     1201 cttcaaactc tttttttcca ctgctactat aagctgttat atcaaaatta atagaattct
     1261 tttttccata gcttattaga ttttttagat gtctctttcc aattgaccct aacccaaaaa
     1321 aaattacttt catcattaat ttacctcaat gtcgttaatt aagttaataa tatacttatc
     1381 tttttcaaaa gtatattggg gttccatttt cttttccaaa agcgtgaaaa attctttaat
     1441 ctcctctaaa tatg
//
LOCUS       DAZUKR010000252         1451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000252 DAZUKR010000000
VERSION     DAZUKR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.260"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACG89C_002867"
     CDS             complement(<1..625)
                     /locus_tag="ACG89C_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS4901339.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACG89C_002868"
     CDS             complement(784..1050)
                     /locus_tag="ACG89C_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4901340.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACG89C_002869"
     CDS             complement(1040..1396)
                     /locus_tag="ACG89C_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4901341.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DAZUKR010000253         1440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000253 DAZUKR010000000
VERSION     DAZUKR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.261"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACG89C_002870"
     CDS             105..1406
                     /locus_tag="ACG89C_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4901342.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAZUKR010000254         1429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000254 DAZUKR010000000
VERSION     DAZUKR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.262"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..830)
                     /locus_tag="ACG89C_002871"
     CDS             complement(<1..830)
                     /locus_tag="ACG89C_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFS4901343.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
     gene            complement(901..1362)
                     /locus_tag="ACG89C_002872"
     CDS             complement(901..1362)
                     /locus_tag="ACG89C_002872"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4901344.1"
                     /translation="MFQILKLSKNEIRHSNFLAFLFAPNETHNLSDEFFKMFLKRYID
                     SNDDTKAAINYFDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQ
                     LNKYQIYIERNYSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
BASE COUNT          468 a          248 c          191 g          522 t
ORIGIN      
        1 taaactaatt tgaaatcgct acagatttct tgtcgatcac taacacgaac cttatgagcg
       61 atatttctag atatatggac acaacattgt tgaaattgag catttggata aacactatga
      121 atactatcgt caataccact taaaccatct gttacgacta acaaaacctc ttctaaacca
      181 cggtctttta gatcttgaag aatctctttc caaacataag cagattcagt tggagctata
      241 gtaaatccta gaacctcttt agttccatcc aacctgatac caattactat atagacagct
      301 tcttttgata cggtttgtct ttttaaggga atatgagtag catccataaa aatgactgag
      361 tatttagctt ctaaagttct ttctttaaat gccgaaacat cttcagacac aattttactc
      421 atatttgaaa tagtttgtgg tttgtaataa tgaccataca ttttttcgat tagatcagag
      481 atttcggaca tagtaattcc tttttgaact agctgaataa tagtggtttc taaagagtca
      541 ttggttcttt tataagcagg taaagtttgt tgagaaaagt ctccattcct atctctaggt
      601 attactaaat tcaattcacc gtactctgtt ttaaaagagc gcgaataatt cccattacgg
      661 gaattacctg aattaaatcc agctctatca tacttttcat agtcaagaaa taccgttaat
      721 tcagcttgta ataaagcgtt aatagccaat tctaggtgac gacgaaacaa atcatccaaa
      781 tcacctttat tgattagtat ttccataatt tctgtagtaa aatcgttcat atggaagtcc
      841 tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat
      901 ttacacaaaa tattttacac tctcttcaac gatattcttg taagtaatta tgccccaact
      961 aggatttgtg ggttcatttc catctggagt taaaaaaaca aaaatttttt tatagttaga
     1021 ataattgcgt tctatatata tttggtattt attaagttgc cctccagact ccgaagacag
     1081 aattttattt tcgatgcaca ccactatttt atttttttcc gataccaata aaatatcaat
     1141 attcttattt tctctgtaaa caattaaatc ttcatagcta ttaagtaaag catcaaaata
     1201 attgatagca gctttggtat catcattaga atctatatac cttttcaaaa acattttaaa
     1261 aaactcatca gataaattgt gggtttcatt tggagcaaac aaaaaagcta aaaaattact
     1321 atgtcttatt tcatttttag atagcttaag tatctgaaat atatttgttt tcccagtcca
     1381 tttagatagt tttcttcaat atcatccata tcctctaata gatttttta
//
LOCUS       DAZUKR010000255         1422 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000255 DAZUKR010000000
VERSION     DAZUKR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.263"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1414
                     /locus_tag="ACG89C_002873"
     CDS             <1..1414
                     /locus_tag="ACG89C_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901345.1"
                     /translation="YVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRT
                     VDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCS
                     ALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSP
                     FKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGV
                     VSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWL
                     IIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAI
                     KERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGK
                     VVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPN
                     KLKDSRYVTKKEINKKNENK"
BASE COUNT          499 a          203 c          251 g          469 t
ORIGIN      
        1 ttatgttcta tatatttggt tattaaatat ttgtacgatt ctgattagtt tttattatgg
       61 agaaaagttt tttaaaaaga tagatgctgc ttttttattt agttgtttat atacttttgc
      121 aacttatcga acagttgata tctattatcg atccgcaatt gcagaagcaa tcgctcttac
      181 gattatccca gctgttttat attatgctta tggattgata ttcaatagag aaaaaaaagt
      241 catcgcttta tctgtcagta taagtctact tgtgtatact catgtcttaa ctacactatt
      301 gtgtagtgca ttgattgctt gtatgattat agttcgtttg tttttaagca agcaaataaa
      361 aaacgatata aaatttgtgg tcgtgaattt agtaaaggct gtttttttga cttgtgcatt
      421 aactagctat ttttggtatc ctatgttgca gcagatgaat tatcaagaaa tacacagtcc
      481 cttcaaaaca gatttgcagc tagaagcact ttctatttca gatagtatca ttcaagcgtt
      541 aaataacgat attacaacat acactatggg gttattagga ttggttgcgt tgatttttcc
      601 tttattcata ctgaaagaaa tggagaaaaa agaaaaaatc atctatgttg gcgtagtttc
      661 tacatgggta tgcgtaacta atttatttcc atggtttttg tttcaaaaaa caccgataag
      721 cttactccag tttccttggc gtatcttagg tatccaaatc tttttttctt caatgatcat
      781 aacgcttgtt tttatgaaaa agactatgaa taaaaaatat tcatggttaa tcattggcgc
      841 tactgtggtg tttctactcg tcacgtctat agcagcaaaa gaaaattacg caaaagttat
      901 ccaatcaaaa cacggtcata ttgttatcaa tgaagaaaca ctcccacgtt atacaacatc
      961 agatatggga ggattatacg attacgcccc aacagaagcg ataaaagaaa ggcctcactt
     1021 agaaaaacat gaagtaaaag taggagaatc gtgggaaaaa gggaagtata aagctgcaga
     1081 taataacatt acttacacag tggcaagcaa caaaaagcaa aaagtgacag taccagtata
     1141 tgcttattta ggtacacagg taaaagtaaa cggtaaagtc gtaaatgact cttataaaaa
     1201 acatggctta gtgaatgtgg atgtaaatgc tggagaaaat aagatagaaa ttacaacaaa
     1261 atatactcca actgctctca tggctttatg gatcagtatt ctgacttatt gtttagtggt
     1321 gtattcaagt tttaaacgat ttgcacctaa taagttgaaa gattctcgat atgtgactaa
     1381 aaaggagata aacaagaaga atgaaaataa ataaaaaagt ag
//
LOCUS       DAZUKR010000256         1417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000256 DAZUKR010000000
VERSION     DAZUKR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.264"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG89C_002874"
     CDS             complement(150..1337)
                     /locus_tag="ACG89C_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS4901346.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZUKR010000257         1394 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000257 DAZUKR010000000
VERSION     DAZUKR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.265"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACG89C_002875"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACG89C_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS4901347.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYRVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          580 a          181 c          244 g          389 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattacaggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttc
//
LOCUS       DAZUKR010000258         1393 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000258 DAZUKR010000000
VERSION     DAZUKR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.266"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..612
                     /locus_tag="ACG89C_002876"
     CDS             205..612
                     /locus_tag="ACG89C_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901348.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            626..1027
                     /locus_tag="ACG89C_002877"
     CDS             626..1027
                     /locus_tag="ACG89C_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901349.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1029..>1393
                     /locus_tag="ACG89C_002878"
     CDS             1029..>1393
                     /locus_tag="ACG89C_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901350.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKL"
BASE COUNT          539 a          175 c          240 g          439 t
ORIGIN      
        1 agtaaaaaaa cagtttgttc cttatataga ggcactttta ctactgatta aatgtcagat
       61 atgcttacaa atatacttat aaatatgctt acaaaattag ctaatatttc cgttgacaaa
      121 gctttgttac tattgttaaa ctaaattgac tagcaaaagt gaaacataaa tttattaaat
      181 aaatgtaaaa attaggagaa atcaatgtct tatagagtac aattaataat ttcagaagat
      241 gtagaaagtc agcaatttgg cactaatgtt attaataaag taattaatcc agctctttct
      301 attaatgcgc ctttaattcc tactgcttta tcattttctg taacagctgt ggtgtctgag
      361 atagaggata cagaaaagat aaaaatagta gaaattgaag ttttaaacaa gaatgaaaaa
      421 caaatttttt ctacgggtga agtatcggta agcttgcctc ctcaagttaa cgatataaac
      481 tttaatataa acgccagaaa tgtcttggtt gaagaagcag gagagcatta tgctgttgtt
      541 aaatttaatg gaactgagat tggaaggcaa atctttgata tcaaggtcaa caaaccagtg
      601 gaaaaaaatt aaggagacga tacggatgct tgatatagta ccatcaaata cacatcggag
      661 tggtagctta ataaattttg ctgcgatttt tgcttgcgca tcggtaattc tagcatctcc
      721 agttgttgtg gatgcaagtt ctactccgaa aactaaaaat gataatcaac ttgtaattac
      781 tacaaaaaat gagattaaca caatttcaag taatgatggc aaaatttttg atgtgataaa
      841 tacggtttta aaaagaaaag ctgaaatcaa tataaaatat gatgaagatt taaacttgta
      901 tttctttgtt atcaaaacaa catctgaatt gttttcctca gattacgatg ttttggatac
      961 tctcgataat gtattatctg actataaata tatggggaaa tctgtagtag caactttggg
     1021 ggaataaaat gtttgacttt aatgaatatt tatctatttc caaacaaatt gaatcagatg
     1081 ataaatataa ttctaaagaa tcttatagaa gaactgctgt ttcaagagca tactacagtg
     1141 cttttaaaaa atctgatgag tatttgaaag aaaattatga tattatttat aatggaagta
     1201 gcggaaaagg aagccaccaa accgtttgga atttgttttc aacagttaag gaattgaata
     1261 ccttaggaat acataatagt gggtatagaa tgttagagaa aagaaaatgt gctgactata
     1321 ttcctaatga aaccattacg aaaacagata tggcgctgat gaatcgagaa gcagaaaaaa
     1381 taataaacaa act
//
LOCUS       DAZUKR010000259         1377 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000259 DAZUKR010000000
VERSION     DAZUKR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.268"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..>1377
                     /locus_tag="ACG89C_002879"
     CDS             287..>1377
                     /locus_tag="ACG89C_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901351.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTG"
BASE COUNT          463 a          136 c          240 g          538 t
ORIGIN      
        1 tctggttata tgaaaaaagt agtttctaaa ataatcttgc agggattaac ttaaagaaat
       61 tgagagaata gtttctttga aatttctata tacaatgaga agctgaaatt tacttttatt
      121 tgatgactgg aaatagaaat taaatatatt gtattgttac attaaagaaa acttgtatgg
      181 aaaaatcagt acaataccat tatttaatgt tcttcactta aagcgcaaaa aatatcagtt
      241 tattaaactc gtatatagaa caaatatatg tagacggggc gaaacgatga gtgtaaaaaa
      301 aatgctattt atggtaagta caataattgt attaacatcc cgattttggg gaattaatac
      361 tagtttatct atgcgatatt ttattatggc aatatgggat ttatatttta tgttaagcta
      421 tttttcttat cgtgagaaaa catataaaaa atacgttatc aagaataact tttggttgac
      481 tataatgccg tttgtgatat tttctatcta tactctaata atatggggaa ttggttcaaa
      541 tgcagaattt gaaaatttta taaagttatg ttccacgttg ctttatttaa ttcttgcata
      601 tggttacgca tgtacagcat tttacttatt taaggcagaa ggaattgatt ttattttttg
      661 gtcaggtgtt atttcatata tgagtggttc agttttttat ttaattgtat cttatggttt
      721 aaatggttta atttcttata taaaaagtct tataacaggt gaaacaaata ctgcaaattt
      781 tgctatggag gtacatgatt taacttttgc gatgggggtt tttttccttt attacgtatt
      841 ttttgaaaat aaaaatacaa aaaaacataa gataaaaatt atcgtatctt taattttaat
      901 tttcatggga ttgaagagaa ttgaactgtt agctttaagt ggtgcaattc tagtgtatta
      961 tgttttatta aagtggggaa aaaagattca aatccgtgca ttagtgtgta ctatttgtgc
     1021 aactgttatt tcgttagtgt ttatttacat tatagataca ggaattttgg cattgcttat
     1081 gtcaaatttg ggcattacag atgacggtgc gaggctttca ttttatctat attcatctaa
     1141 attttatgaa cttggtttag gatatgtagg aacaggatgg acatggtttt caaaatactt
     1201 ttttaatctt tatttatcgc actttaggtt aaatggtgga agaattgcag caagcctaca
     1261 cagtgatata ttaactattt atatagaagt tggatgtata gtttttatta tatggatgtt
     1321 ttggttattt tttgtgaaac cgataagact aactaaaaaa tttggattga aaacagg
//
LOCUS       DAZUKR010000260         1372 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000260 DAZUKR010000000
VERSION     DAZUKR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.269"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            543..>1372
                     /locus_tag="ACG89C_002880"
     CDS             543..>1372
                     /locus_tag="ACG89C_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4901352.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          500 a          182 c          223 g          467 t
ORIGIN      
        1 agatgaagcg acaagtggac aattaataaa ctacttgaac atgaaattat ggatcacctt
       61 tcaaatttag aaaatggaat tcttatactt attgcgcaac atttattgat tgcaaaatta
      121 tgtgacaaaa aaaactagta gaagaaagaa taatggcttg ataataaagt actacctatt
      181 aaaggtagta ctttatttaa tttagtataa attaacatac tactatattt taggtttcat
      241 attatataaa ttaataatat actaccattt gttggtagta taatttaaag gtaataagtt
      301 attcttttat tactatgttt gggttttata gcaaagaaag tttaataaga gtttggacct
      361 agcaaacaat attaatgagg tcattaaatc ttattatctt tagtgatata acgaaatatc
      421 tacacagaca aaatgatagc gtaaaatatt ttgtgtaaat agatatttag ggtagaaaaa
      481 aagaaagtcc attctgtaaa attaaagtta ccacaaccga aattttatag gaggactttc
      541 tcatgcccaa ttttactaca gaaattatgg aaacactaat caataaaggt gatttagatg
      601 aattatttcg atgtcattta gaattagctg ttaattcatt attgcaggct gaattaacag
      661 cttttcttga ttatgaaaaa tatgatcgtg ctgggttcaa ttcaggtaat tcccgtaatg
      721 ggaattattc acgttcattc agaacagagt atggagaatt aaatttggta attcctagag
      781 atagaaatgg taagttttct caacaaactt tacctgctta taaaagaacc aatgactctt
      841 tagaaaccac tattattcag ctatttcaaa aaggaattac tatgtctgaa atctctgaat
      901 tgattgaaaa aatgtacggg catcactata cgccacaaac catttcaaat atgactaaaa
      961 tagtttctga agatattatt gcctttaaag aaaggtcctt agaatccaga tactctgtca
     1021 tttttatgga tgctactcac attcctttaa aaagacaaac tgtttcaaaa gaagcagttt
     1081 atattgtaat tggcattcga ttagatggaa ccaaagaggt tcttgggttt agtcttgctc
     1141 caactgaatc tgcttatgtt tggaaagaga ttcttcaaga cctaaaagac cgtggtttaa
     1201 aagaggtttt attagtcgta actgatggtt taagtggcat taacgatagt attcatagtg
     1261 tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata
     1321 aggttcgtgt tagtgatcga caagaaatct gtagcgattt caaattagtt ta
//
LOCUS       DAZUKR010000261         1370 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000261 DAZUKR010000000
VERSION     DAZUKR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.270"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..134
                     /locus_tag="ACG89C_002881"
     CDS             <1..134
                     /locus_tag="ACG89C_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4901353.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(220..1314)
                     /locus_tag="ACG89C_002882"
     CDS             complement(220..1314)
                     /locus_tag="ACG89C_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS4901354.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          416 a          287 c          211 g          456 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
      181 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
      241 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
      301 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      361 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      421 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      481 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      541 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      601 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      661 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      721 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      781 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      841 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      901 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      961 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
     1021 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
     1081 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
     1141 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
     1201 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1261 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1321 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac
//
LOCUS       DAZUKR010000262         1355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000262 DAZUKR010000000
VERSION     DAZUKR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.271"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG89C_002883"
     rRNA            <1..1355
                     /locus_tag="ACG89C_002883"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZUKR010000263         1351 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000263 DAZUKR010000000
VERSION     DAZUKR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.272"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACG89C_002884"
     CDS             202..1077
                     /locus_tag="ACG89C_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4901355.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..>1351)
                     /locus_tag="ACG89C_002885"
     CDS             complement(1222..>1351)
                     /locus_tag="ACG89C_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HFS4901356.1"
                     /translation="CQNFIQYTDAVEPLLQNVEKYHKIYQQCRKIYPATKEICHDL"
BASE COUNT          487 a          247 c          225 g          392 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca a
//
LOCUS       DAZUKR010000264         1351 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000264 DAZUKR010000000
VERSION     DAZUKR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.273"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1243
                     /locus_tag="ACG89C_002886"
     CDS             <1..1243
                     /locus_tag="ACG89C_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4901357.1"
                     /translation="ILSKWKIYYYGINFLIMGNTQVHLLKSWRTNSLITVILGISLMT
                     IGGTASISTILSYQEKVSSPMDYQFDVETEKKIKPILAEEGQKITKEITLHYKVIGSI
                     YSQNLEEPMFQTANLITETQYQAFRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLY
                     GSSIGLPDNQELKIQQMLPSVLGDETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSG
                     GNNEKIDERLSNDLSIKWLNAVYYSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSR
                     LNFSSSYSKLRAVNRRIGINIFVTLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLT
                     KLGIPQRRIRRMIFEQNALTFFPPMILGITHAVFAINVFTQYIKTADYWLAYLVSGLL
                     ILIYLLLYVITSQLYCRIIEE"
BASE COUNT          443 a          225 c          262 g          421 t
ORIGIN      
        1 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
       61 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
      121 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
      181 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
      241 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
      301 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
      361 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
      421 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
      481 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
      541 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
      601 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
      661 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
      721 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
      781 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
      841 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
      901 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
      961 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
     1021 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
     1081 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
     1141 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
     1201 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
     1261 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
     1321 tttttgtcac aacctcttta ttttttatat t
//
LOCUS       DAZUKR010000265         1320 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000265 DAZUKR010000000
VERSION     DAZUKR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.275"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..>1320
                     /locus_tag="ACG89C_002887"
     CDS             80..>1320
                     /locus_tag="ACG89C_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS4901358.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKS"
BASE COUNT          447 a          258 c          251 g          364 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
//
LOCUS       DAZUKR010000266         1294 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000266 DAZUKR010000000
VERSION     DAZUKR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.276"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG89C_002888"
     CDS             <1..1244
                     /locus_tag="ACG89C_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4901359.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZUKR010000267         1287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000267 DAZUKR010000000
VERSION     DAZUKR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.277"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACG89C_002889"
     CDS             <1..848
                     /locus_tag="ACG89C_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4901360.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DAZUKR010000268         1282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000268 DAZUKR010000000
VERSION     DAZUKR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.278"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..243
                     /locus_tag="ACG89C_002890"
     CDS             <1..243
                     /locus_tag="ACG89C_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4901361.1"
                     /translation="SRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLI
                     LDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            248..1195
                     /locus_tag="ACG89C_002891"
     CDS             248..1195
                     /locus_tag="ACG89C_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4901362.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          420 a          185 c          267 g          410 t
ORIGIN      
        1 tctaggcaac agacacctga agtttttgaa atgaatgcct caatgtattc ttttaaacct
       61 gaatttctaa aaaaaagtaa ttatattttt gatgggcaat gtgggataat aaaaatgaga
      121 gattatctaa ttttagatat tgatagtgaa gaagatttta aatggttaga atttttgttt
      181 cctaaatttt tagaagattc ttcggagctt caagatatct ataattattt atatcggagg
      241 taaaaggatg agctgttatc ttatcaccgg tggggctggc ttcattggtt ctaccttagc
      301 caatcattta ggagaagaac atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt
      361 agaaaatctt gatatgacga aaaatatcac ttttattgag ggagatgtcg ctgagaaatc
      421 cttgatggaa gagatcatga aagctaataa atttgattat atctttcatt tagcagcagt
      481 tgctagtgtg gcagattcag ttgcccgacc agtagaaaca catcgtgtga attttgaaag
      541 tgtattaatg attttagaat tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt
      601 tagttcttca gcggctgttt atggggatga accgacttta ccaaaaaaag aagagtccgt
      661 gattcgccca ttgacgcctt atgcggttga taaattcgct gcagaacaat atgtgctaga
      721 ttattgtcat ttatatgatg tacccggtag cgcagttcgt tttttcaatg tctatggtcc
      781 aaatcaaaat ccgaattctc catacagtgg tgtgatttct attctcgtag atcgctataa
      841 gaaacaaatc gtaggagaag caacttcatt tacattattt ggtgatggaa gccagtctag
      901 agactttgtt tatatagatg atgtaatcca agcattactt ttagttgcga atgaggaagc
      961 tgcactgggg aaacaattca atgttggaac gggtaaagca accacacttt tggaattgat
     1021 tcatgcaatc aatgaaattc tcggtgtaga attgacactt gaatacaaag aagagcgttc
     1081 gggtgatatc catgactctc tagcagatat tactaagatc cagtcaattg gctatcaacc
     1141 gaaatatggt attttaagtg gaatgactaa ttatttgaaa actgaaatca attaaaacat
     1201 actaagataa gattagaggg taaattcagg ctctttgtca ataaggactg atgagttttg
     1261 taaaatcaaa tctgggatag aa
//
LOCUS       DAZUKR010000269         1269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000269 DAZUKR010000000
VERSION     DAZUKR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.279"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1103
                     /locus_tag="ACG89C_002892"
     CDS             24..1103
                     /locus_tag="ACG89C_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4901363.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          302 c          310 g          279 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg
       61 gaaaacatct ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt
      121 ccaaccgtca ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg
      181 atcgcggaac catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct
      241 actattctgt ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact
      301 gtggcagacg ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa
      361 tgttaaatga aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt
      421 tcgattgtgc catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca
      481 tgcgaacaaa gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc
      541 caggacaccg tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag
      601 ttgaaacacg ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca
      661 agtcggatgc cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa
      721 agttgaacgg gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg
      781 ctggcgctaa cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt
      841 ctgggctaca cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt
      901 cttgggagcg tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg
      961 gaagcccgat tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg
     1021 attacccgcg gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca
     1081 agcaagaacg gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat
     1141 ggcttcatgt ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag
     1201 ttcattatca aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga
     1261 gttacctgt
//
LOCUS       DAZUKR010000270         1258 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000270 DAZUKR010000000
VERSION     DAZUKR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.280"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1190
                     /locus_tag="ACG89C_002893"
     CDS             150..1190
                     /locus_tag="ACG89C_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4901364.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          506 a          178 c          206 g          368 t
ORIGIN      
        1 agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat
       61 gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa
      121 taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata
      181 gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca
      241 aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg
      301 caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg
      361 aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg
      421 aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata
      481 atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg
      541 aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac
      601 ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca
      661 taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata
      721 atatattaga cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat
      781 tgttgttaga caattttgag acaattatga caaacgacag cattgctacg tttgtccctg
      841 aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg
      901 tccagactat tcataatgca aagaaaaaag ctgaaataga aagtggtatt tcgatagttt
      961 ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa
     1021 aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct
     1081 atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt
     1141 tgaattatga aagctcccca agcactatta cgaaagactg gttagagtga aactttactg
     1201 acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aaatttttta ttattcta
//
LOCUS       DAZUKR010000271         1254 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000271 DAZUKR010000000
VERSION     DAZUKR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.281"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..828)
                     /locus_tag="ACG89C_002894"
     CDS             complement(<1..828)
                     /locus_tag="ACG89C_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFS4901365.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     "
     gene            complement(834..>1254)
                     /locus_tag="ACG89C_002895"
     CDS             complement(834..>1254)
                     /locus_tag="ACG89C_002895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4901366.1"
                     /translation="GTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLP
                     SLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDIS
                     HRDSERSILIKADYNIQTAYKILESKYYELFELIQRG"
BASE COUNT          384 a          237 c          145 g          488 t
ORIGIN      
        1 aaatgtctgt gctatttcat gatttattct ctgtgctaaa acaatcatat aatctatcat
       61 caaataatcc accatcatat cattattttt ccaataattt aaaaaaaagt ctctaattat
      121 agcaaacatc catctagact cttgattaca tgctaatgaa taacccgcaa aatatcctga
      181 tgctacggat gcgtgagcat aatcaggcct cctaatagac caaatatcga tatcaaaata
      241 ctcatctggt attggttttg tacaataaaa tgtggcgtct aaccacaaac ctccatgttt
      301 tgctagcaaa gataatctta ataaatcaga ataatttgtt ctcgtaatga ttcctttgtt
      361 tttcttttct tccacccatt ttggtatatc aacatattgt ctataattat cttctgttaa
      421 aattataaca gtatgtttcc ctgcatttcg ttttattgat tttacacaag cttttacaat
      481 ttccggcgat tgatccaatc cctgccacca acaaacccaa atgcagttag aatatggtga
      541 ttttgattga ttaattattt gattttgact ataatcatat gttttcaaaa aatctgaaaa
      601 agtcttttca taatacttca gcattacctc atgttttttt aataaatgtt ccttctgttt
      661 tttactttca ttatatccat tacgattcat gacttgaata tcaattttag cttgtaacgt
      721 ggcaaatgct tccttccaag atgttatctt tgcaattcgt cttgttgcat tcaattcttc
      781 ggatatgcga tgtataattc tttttatact tgaatgtttc tgtgtcatat tctttaacct
      841 ctttgtatta attcaaataa ctcataatat tttgattcta agattttgta tgctgtttga
      901 atattataat ctgcctttat taaaatcgaa cgttctgaat ctcgatgaga gatatctaca
      961 tttgcaatct ctctcgccca ttcttttgcc gattgattta aacttatgaa attggtctta
     1021 tcactgaaag caacttctgt aggaactttc tccgaaaaga aacacggtaa atctgcaaat
     1081 tgagcttcaa tacccactaa cggaacccct tcaaataatg atggcaacat taatacgtca
     1141 aatgcttgat aaaatttttc tatatcattt ctatttccta aaaatttaat atcatcatct
     1201 atgccacttt tctttgcata attttttatt tcttcttcca tatcaccagt tcca
//
LOCUS       DAZUKR010000272         1246 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000272 DAZUKR010000000
VERSION     DAZUKR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.282"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG89C_002896"
     CDS             200..742
                     /locus_tag="ACG89C_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901367.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG89C_002897"
     CDS             898..>1246
                     /locus_tag="ACG89C_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS4901368.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZUKR010000273         1210 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000273 DAZUKR010000000
VERSION     DAZUKR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.283"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..1015
                     /locus_tag="ACG89C_002898"
     CDS             8..1015
                     /locus_tag="ACG89C_002898"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS4901369.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1027..>1210
                     /locus_tag="ACG89C_002899"
     CDS             1027..>1210
                     /locus_tag="ACG89C_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFS4901370.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDN"
BASE COUNT          477 a          157 c          230 g          346 t
ORIGIN      
        1 gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt ccattggaag
       61 tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt atagtagagg
      121 tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt tgagattttt
      181 aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg tggatattat
      241 ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta tggaagctgt
      301 aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa
      361 acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca tgggtgcgac
      421 aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa aaacagaaac
      481 aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg ttattcctct
      541 ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg atatgacacg
      601 atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta tgaattcaaa
      661 atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt tagctcaatc
      721 attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa taaaagttgt
      781 aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg aaattgaacg
      841 ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta ctgacgaact
      901 gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa cagaattaat
      961 gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt
     1021 tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca taatggaagt
     1081 ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa cgcggttaaa
     1141 tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc aaaatatcag
     1201 aaggataatc
//
LOCUS       DAZUKR010000274         1193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000274 DAZUKR010000000
VERSION     DAZUKR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.285"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG89C_002900"
     CDS             <1..969
                     /locus_tag="ACG89C_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS4901371.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          400 a          214 c          241 g          338 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctc
//
LOCUS       DAZUKR010000275         1178 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000275 DAZUKR010000000
VERSION     DAZUKR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.287"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..>1178
                     /locus_tag="ACG89C_002901"
     CDS             175..>1178
                     /locus_tag="ACG89C_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4901372.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     "
BASE COUNT          479 a          110 c          231 g          358 t
ORIGIN      
        1 aaaaatttaa ataatatata tgatgaaaaa gtatggaact tattaaagaa agatacttta
       61 ctgtttaagt tgacatggaa acaatctttt aaaaagcaaa aagatgataa agttactttt
      121 tatgggatgt tggtagaaaa caaattatta taaatattag aggtataaaa agttatgaaa
      181 cacaaggtaa gtgtgatagt accaatttac aatgttgaaa aatatttaag aaaatgtgtg
      241 gatagtttat tgagtcagac attaaaagag atagaaatcg tattagttga tgatggttcg
      301 ccagatgctt cgggggaaat agcagatgag tatcaaaaaa aatattctaa tgtaaaaaca
      361 atacatagag aaaatggggg gttaggaccg gcaagaaata caggaattga gaatgcgaca
      421 ggagaatatg tggcttttct tgattcagat gattgggtag aaagtgatat gtatgaaaaa
      481 ttatacttag ttgcgtccaa agaaaatgct gatatagtag ttagtggaca ttgtgatttt
      541 gcgaatgaca aagctatggt gaaaaagcaa catcctttga gtgggaatgt atatgatacg
      601 cctaatagta ttaaaaatgt acgaaaaaat ttatttggac atagtccaaa agatcaagaa
      661 gtagaggcct tcccgatgtc agtatgtatg tctttgtata ataaaaatct gctagataaa
      721 tatagactaa gatttgaaaa aattttatct gaagatacaa tttttaactt gaatgcatat
      781 gatgtcgcaa ataaaattgc ttttacagaa tatacagatt attgttatag aaaagaagaa
      841 caagagtcta ttacaaaaag tttatctcca aataagcaga cggaatttaa agaattttta
      901 ataactctaa aagctatagc atcgaaagaa gatttagaat gtcaaatcag agcaaaacgt
      961 atggcaattg attattgtag gctttatgtg ggcttagttg atgatgcaaa tttgggaatt
     1021 aaagagaaaa gaaagtatat aaaaaaattt attgaagatg agggattaat tcaatgttgg
     1081 aagggatatc ctacaaatac gctaccattt caacaaaaat tttttcataa aatgcttgaa
     1141 tgtaagtgtt atacaatatt gttgttgtta agtagaat
//
LOCUS       DAZUKR010000276         1160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000276 DAZUKR010000000
VERSION     DAZUKR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.288"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG89C_002902"
     CDS             14..1141
                     /locus_tag="ACG89C_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4901373.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa
//
LOCUS       DAZUKR010000277         1142 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000277 DAZUKR010000000
VERSION     DAZUKR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.289"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..>1142
                     /locus_tag="ACG89C_002903"
     CDS             199..>1142
                     /locus_tag="ACG89C_002903"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS4901374.1"
                     /translation="MIVSYIIYVWLLNLCTTLVAYHYGERFLKQKKAAFLFSCLYIFS
                     AYRTVDIYYRSAIAEAIAITLVIPVLFYAYQIISGKEEKYPSVKLALSMSLLVYSHVL
                     STLMSTALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLVLTSAFWYPMFEQMLY
                     QKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGLLSLCIPLILFKKLTNIEK
                     KIYYGTCLTWLATTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIFFKNRRY
                     NKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIV"
BASE COUNT          357 a          182 c          179 g          424 t
ORIGIN      
        1 atctttttca aaaatacaca gagtatttat accactggag atctttcttt tcacttatct
       61 cgaatcaagg ggttatcgac tatttttacc agtccgatta attatgagac attcaattat
      121 actggatatg gtgtaaatta tttttatccc tttctgactt tttttccagc tgtaatgtta
      181 tattggatga caaaaaattt gattgtaagc tatattattt acgtttggtt attgaacttg
      241 tgtacaacac tcgtagctta tcattatggc gagcggtttt taaaacaaaa aaaagcggca
      301 tttttattta gttgtctcta tattttctct gcttatcgga cagtagacat ctactatcgt
      361 tccgcaattg ccgaagctat cgcaataaca cttgttatcc ctgtattatt ttatgcttat
      421 caaatcatct ctggaaaaga agaaaagtat ccgtctgtaa agctagcatt gagtatgagt
      481 ttgctagtgt acagccatgt actatcaaca ttaatgagta cagcattaat tatcattttt
      541 atttttattc gtttggtttc taaaggtttc aagaatgcag attttattgc tatatttaaa
      601 aaattattta gtgcggcagg aatgacattg gttctgacga gtgctttttg gtatccgatg
      661 tttgaacaaa tgctctacca aaaaatcaac aaacctagcg tgacaaattt atatgctcat
      721 gcatctaatg tgttcgatag tctgactgaa gctatgaaca acgacttaac tacttactca
      781 atgggactag ttggtctgtt atctttatgt attcccctga ttttatttaa aaaattaaca
      841 aacatagaaa aaaagattta ttatggtacg tgtttgactt ggctagctac aactagtttg
      901 gtgccatggt atcttctgca aaatacgcca gccaaattgt tacaatttcc ttggcggatc
      961 ttaagcttgc aaataatatt ctcttctctg atattaacga tgatattttt caaaaataga
     1021 agatacaata agacaagaga actattttat ttatttatga ctgttttgtt attaatcact
     1081 ttaactgttt ctagtaaaaa aaatttcaat caaaaaataa tttctcagcc gggacatatt
     1141 gt
//
LOCUS       DAZUKR010000278         1124 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000278 DAZUKR010000000
VERSION     DAZUKR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.290"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1118
                     /locus_tag="ACG89C_002904"
     CDS             90..1118
                     /locus_tag="ACG89C_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901375.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
BASE COUNT          417 a          170 c          208 g          329 t
ORIGIN      
        1 ttttgaaaaa ctatcattta tcttgcagct tgaaaaaagg taatccgata gaaggagaag
       61 aataccgtat ccgatacttt tttcacttga tgtattggca gtcattcgac gaaaatcatt
      121 ctgatatatt atcaagtata aaaaataaac gtcaactatt agaaaaattt gcgaaacatg
      181 cgccatatac gcgtagtata gatagaaaga aatggatcag cctactaaga atttcgttgc
      241 aacgaatgaa acaaggccac tcgattacgt atgtcccaga agagatgaac aaatttgtgc
      301 atccattgat aacaaaagaa gagtttacgc attacatact agagcctttt tttgaagcaa
      361 acttctggtc gatgaatcac atcactccaa cagagcaagc ttacctttat tttatgtttg
      421 gtgtaatgaa cacctatttg gaagaagatt tgttggatac tccagataaa gtacaagaaa
      481 gttggggctg gattgaatcg tctattgtag aaaattttga aaaaaacttc aagatttgtt
      541 ttacaacttc agaaaaaaat tatctatatg tcaatttggc aatgatccat ttgtattcaa
      601 gggtttttgg gacaaaaaag aaggtggatg cttttggaaa atcagcagaa gacagagatt
      661 ttcagcatat atttccagtg atgtatccaa tcatgcaaca gttttttagt ggggtagctc
      721 gcagaattcc agagcttaat acttattatg aaaagaacag acggttgatt tttcaatact
      781 gtatgttagt cagggatatt tttataaaac acgaacaacc tgtcatattc tatattcaat
      841 caaagtatgg taaaacacag gaattatggg caaagaaaag gatattatct atgactgaac
      901 gcccaataaa atatagtgca tccgctgatc agttacctga tattgtcatt agcgattatc
      961 ctatcgacaa aaaggcatat gatcaaacga atacacagat attttactgg aatggacaac
     1021 ctacaaaaaa tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa
     1081 atagatccat attggaaaaa ttggatttcg ttaattaaga aaag
//
LOCUS       DAZUKR010000279         1116 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000279 DAZUKR010000000
VERSION     DAZUKR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.291"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACG89C_002905"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACG89C_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS4901376.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DAZUKR010000280         1111 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000280 DAZUKR010000000
VERSION     DAZUKR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.292"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..400
                     /gene="spx"
                     /locus_tag="ACG89C_002906"
     CDS             <1..400
                     /gene="spx"
                     /locus_tag="ACG89C_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFS4901377.1"
                     /translation="YTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAA
                     TEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFN
                     EDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            414..770
                     /locus_tag="ACG89C_002907"
     CDS             414..770
                     /locus_tag="ACG89C_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4901378.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          384 a          170 c          211 g          346 t
ORIGIN      
        1 atatacttcg cctagctgta cttcctgtag aaaagcaaga gcatggttgc aagcgaatca
       61 acttgaattt gaagagaaga atatttttgc cgaaccgtta acagaaagtg agatcaaaaa
      121 gatcttggca gcgactgaag gaggagttca agatattatt tccactcggt cgaaaattta
      181 cgaaaagctg ggcatagatt ttaacgaatt aactttaaag caaatgatcg tcttattcaa
      241 agaatatcct tcattattga gacgacctat tttgattgat gaaaaaagaa tactaatcgg
      301 atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa attcgatgtg ttgccagaaa
      361 tctgatctat ttacaaaaca ataacatctt aaattcatag ggagggagcc ttcgtggaag
      421 tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa ggcgaaacaa tggttagaac
      481 accatcaatt gccttatcaa tttgttgact tagaagaacg cgatctaagt acagaagaag
      541 cagaagaatt atgcagtttt gaagaagtgc aagcagaaca gctatttgcg acttggtctg
      601 aggcttttca caacattgcg attgaccttt cctgtgtagg aaaaaaacaa ctgatttttt
      661 tatgcacaca acataccaat ctcctcagac gtcctttgat tattatcgat gaagttctat
      721 ttattggata caatgagcag ttgatggaac agcttatctt aaataaatag actttaactg
      781 ttttaatcaa gtcactgagc atttcataca gttgtaacag tcgaacactt agcatcaagc
      841 gtgttatgtg ttcttttttt gactttattt ttctactata gagacatgct caatttatgt
      901 ggtcaaaatt caaatctagg tggggatata gagttaaaaa tatattgaca tgtgtaaatt
      961 tacatgatat attcatttct gttgttttaa gtagcaaagc aataaaatag tattttaaaa
     1021 aacatataaa atagttgttg acatgttaga aatgacatga taagataact gagttgttaa
     1081 aaaagaacca cttgcgaagc ttgattctat c
//
LOCUS       DAZUKR010000281         1103 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000281 DAZUKR010000000
VERSION     DAZUKR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.293"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..>1103)
                     /locus_tag="ACG89C_002908"
     CDS             complement(78..>1103)
                     /locus_tag="ACG89C_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4901379.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          360 a          181 c          166 g          396 t
ORIGIN      
        1 aatggaaagt tttgtctcgc ttaatttgac aaataatatt tttagaaaaa gcagtcggac
       61 tttctaaaag aagttcacta ttcgatcctg tcgatgattt cttcaggaaa atcgtctatt
      121 tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga tagtcaacaa aatctttgta
      181 agattccccc agtctactgc tcttggcgga tcgagccaaa ccactacttg agatgcactt
      241 tcttcatata gattattcct attcgtgatt ataacatccg ggaactcagg ctttcctgcc
      301 tctatcactt ccgtgttcag attaatgtac aagcttaaat ttttcttgat aaattgatta
      361 taggcagcac cataagaaaa atcgatgatg atctttactg gttcagaaat cagttgaatg
      421 ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa gaaaaaattt atcttgaagc
      481 aactctttgc gttttgacgt tagatcattt atatactcta tcaaatagca ataaaatgct
      541 ggatagtttt ctctaaaaaa agaaacatca agtggaaatt catcagcttt attaatagtg
      601 taatgcaaaa gtttaaaatg tattttggtt aactctcttc ggatcaattg gctcgtctct
      661 tctcgcaaca gattaaactg attttcaaaa ctttcaatga atcgttcatt caaccattcg
      721 attgtttcat cttcattttt tctcaagtat ttttgatcca atttatcatg aagataaagc
      781 caagagagtg tcttattgat aaaaataacg gatttttctc ctaatactcc ttttaagtgg
      841 taaaatccta tatccgaaaa aaatagcgac tgattgctag caatatctaa agaatcagct
      901 tgttcagcca acaggttgat tgctaaaaag tggaataatt tctttttttc ataagcagac
      961 agaggataat actgttttaa atcttttaac attttttctg tttttataat tataggtagc
     1021 gtatacaatt ccacaaaact tatctctgtt gagttaaaaa gattatataa aaaaagccga
     1081 atgtttttag gattccctgt caa
//
LOCUS       DAZUKR010000282         1091 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000282 DAZUKR010000000
VERSION     DAZUKR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.294"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1091
                     /locus_tag="ACG89C_002909"
     CDS             <1..>1091
                     /locus_tag="ACG89C_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFS4901380.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPY"
BASE COUNT          348 a          218 c          214 g          311 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta c
//
LOCUS       DAZUKR010000283         1063 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000283 DAZUKR010000000
VERSION     DAZUKR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.295"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACG89C_002910"
     CDS             <1..153
                     /locus_tag="ACG89C_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4901381.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            207..575
                     /locus_tag="ACG89C_002911"
                     /pseudo
     CDS             207..575
                     /locus_tag="ACG89C_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..944
                     /locus_tag="ACG89C_002912"
     CDS             681..944
                     /locus_tag="ACG89C_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4901382.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1063
                     /locus_tag="ACG89C_002913"
     CDS             944..>1063
                     /locus_tag="ACG89C_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4901383.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPY"
BASE COUNT          407 a          132 c          215 g          309 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tat
//
LOCUS       DAZUKR010000284         1042 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000284 DAZUKR010000000
VERSION     DAZUKR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.296"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            409..1042
                     /locus_tag="ACG89C_002914"
                     /pseudo
     CDS             409..1042
                     /locus_tag="ACG89C_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          359 a          183 c          186 g          314 t
ORIGIN      
        1 ttattattaa taaaatattt tttatattac aaaagtagtg tttcaaagtt agtagagaaa
       61 atgtagttat atatttaaat ttttaccaga aagaaatgaa caccttcaat tatctaaatc
      121 tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta ctattcatgg
      181 tcttagacac acacatgcta gcttgctttt agcaagctag tgcaaatatt aaagatgtgc
      241 aagagcgttt aggccactct tctattcaaa tcattatgga tctatatatt catattacag
      301 acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata taggctctat aatttatggg
      361 actgatgaat caaaattgat tcttcagtcc cattgttatt ttaggtatat gattcgtaca
      421 ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc aaaagtattg
      481 atggtggatg aatttcgttc gcacgcttca attgtggata aaatgagctt tatttgtgcg
      541 gatggcgaga ctggaaaact cattgatatt ttgcctacac gaaaattgtc tcgattaaca
      601 cactatttta gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac agatatgaac
      661 gccgcctact tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt cattgattgt
      721 tttcatatca tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca agaaatgaaa
      781 agattacacc agttagggca acgacgggaa gccgagaaag tgaaaaagaa ctggcgcttt
      841 cttctaaaaa accgtcaaaa aatcaatcac tatgattaca aaacatggcg cagttttcgt
      901 gcacctaaat acccgctact cactgaagca atgatgattg atcgattgct ggcattttca
      961 acgcctttaa aggacgccta tacttttttc catgagctga ccgaagcctt tagagacaag
     1021 gaatcagact tattttttac ct
//
LOCUS       DAZUKR010000285         1035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000285 DAZUKR010000000
VERSION     DAZUKR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.297"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..>1035
                     /locus_tag="ACG89C_002915"
     CDS             163..>1035
                     /locus_tag="ACG89C_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4901384.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          360 a          180 c          189 g          306 t
ORIGIN      
        1 gttgatttac caacatgttt agacattttc ctctaggaaa aaattaacta gcaccacggg
       61 tttttattac gccgattgta aaattaagct agacaactaa aaaagtcatt tgtgctacac
      121 tcaaaatagt tccgaccaaa gaattataag gagtgagtac aaatgaccta tacccatctt
      181 acaacggatg aacttgtaat aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa
      241 actgcccatt gcttgaatcg ttcaagacaa acgatccata aagtatatct gttcttcaag
      301 caaggaaaat cagccttaga atattatcaa cagtataaga aaaacaaatc aaactgtggt
      361 agacgtccgc ttgttttacc cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa
      421 ggatggacac ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt
      481 accatttatc gtatgttcaa aaaggggcta tttgattctt ctgacttacc gatgaaaggc
      541 aagcgtaaac cgaatggaca tcaagaaaga cgtggaaaac aaactttccg ccgctctatt
      601 catgaacgtg aaaaggatta tagccaattc tcaaatgagt ttggtcacct tgaaggtgac
      661 actatcgtag gtctgaaaca taaaagtgct gtaattacct tagttgaacg attatcaaaa
      721 gttatcatca cattgaaacc gtgtggtaga caagcgattg atattgaaaa aaaattaaat
      781 caatggtttg aatctgtacc gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag
      841 gaattttcaa attggaaaca gatcagtaat gtcaatgata ttgccattta tttcgctgat
      901 ccaggaacgc cgtctcaaag aggcctaaac gagaattcta acggattgtt acgtagagat
      961 ggtttattga aatctatgga tttcaattca gtagatgaat tttttattca atctgtcgca
     1021 tctaaacgaa ataat
//
LOCUS       DAZUKR010000286         1029 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000286 DAZUKR010000000
VERSION     DAZUKR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.298"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG89C_002916"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG89C_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4901385.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          316 a          202 c          154 g          357 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaacc aattatgaat tgacaaataa aacctgtttt catataataa
      961 tcttaatttt tagaattttt agataattac aaaaaagcaa agggaatcgg tagaaatact
     1021 aagaggaac
//
LOCUS       DAZUKR010000287         1007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000287 DAZUKR010000000
VERSION     DAZUKR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.299"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..170)
                     /locus_tag="ACG89C_002917"
     CDS             complement(<1..170)
                     /locus_tag="ACG89C_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS4901386.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTS"
     gene            328..591
                     /locus_tag="ACG89C_002918"
     CDS             328..591
                     /locus_tag="ACG89C_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901387.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            615..803
                     /locus_tag="ACG89C_002919"
     CDS             615..803
                     /locus_tag="ACG89C_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901388.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT          370 a          132 c          194 g          311 t
ORIGIN      
        1 gacgttgttt cccaatgcct aaaagaaaaa atttgataag taaaatatac agcaggtaaa
       61 gaggccagta aaactgaaaa cttctttttt gtccatttta tgtaatagaa aagaatgctt
      121 atcataataa aaaaaggaat ataaaaaaca aattgaccga tcgcgatcat actattgcct
      181 ccgataaata aagtccatga tacaaaataa cactttgtgc gaaatggcgc aagcagtttt
      241 gtggatttat ccggtaaaat ctgtatcgat tttgaaatta gaggagaaac ttgctataat
      301 caaagtgaaa gaaggagtgg gattttcatg gatcatgtag tgttagaaat cgatttgcat
      361 ttgatcaata atattcgggt gatttactat gttgtaagta acagtgtcga acaacgtgta
      421 ctgacgaata aaataaatgg tattttagca aagaaagacg ttcaccgatt caataatggt
      481 gaaggaagtt attatagtat cccagtagaa aaaattattt atacgacagt caaagtcaga
      541 gaagatctag ataccaaaaa agcatatgaa cctattttta caacttattg attgacagca
      601 gatggaggaa aaaaatgaat ccatatgaac aacttattca agatttgatc gatggaaaaa
      661 tcgaaaagat tgatgtaaaa agagaagaat taatggcttt tcgagaagcg tggctgaagt
      721 tggagaatcg taaatatgtg gtgggaaaag cctcactgaa cggcaatgtg acttatcatt
      781 acgatccgac acgtttattt tagtatgaag tattcagaaa agaaccaaag gaggacgatg
      841 cagcagcatt gtcctccttt gattttacaa aagtgaaagt tttacttatt tgaatagatt
      901 tttaaatttg tttttgacac tgtcgatatt tacatctttt aaaagatttt gggctttctc
      961 tttataaggt cctaattgtt ctttatgttc ttcgataaat cgttttg
//
LOCUS       DAZUKR010000288          997 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000288 DAZUKR010000000
VERSION     DAZUKR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.301"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACG89C_002920"
     CDS             complement(26..913)
                     /locus_tag="ACG89C_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4901389.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAZUKR010000289          994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000289 DAZUKR010000000
VERSION     DAZUKR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.302"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACG89C_002921"
     rRNA            complement(<1..>994)
                     /locus_tag="ACG89C_002921"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAZUKR010000290          992 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000290 DAZUKR010000000
VERSION     DAZUKR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.303"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..327
                     /locus_tag="ACG89C_002922"
     CDS             67..327
                     /locus_tag="ACG89C_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901390.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(689..928)
                     /locus_tag="ACG89C_002923"
     CDS             complement(689..928)
                     /locus_tag="ACG89C_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901391.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          356 a          142 c          181 g          313 t
ORIGIN      
        1 ttcacaatca tattttaatg gtattatcat tttgaataat gaaaaaacat taaggatgga
       61 tgaaatatga ataacgaaac tatcatttta ggaaaaaaat atatttgcca acctattggc
      121 ttgaaacaaa aagtggttgg agaagtaatc agtaaattag aaaattgtgt tgtgatctgc
      181 gtagacgaat atcatgacgt cgatcgcaat gaaatattag aaaaatgcgg aaaagtagtg
      241 gcaaaatata ctcacgttta tgagttggaa acaacagaat atctatataa ctatagcaac
      301 aaatatgaac atactttagt ttcataaaga tttaaataaa gtgccttcaa aagcgagaag
      361 accaagcttt tgaaggcact ttatttagtt ggcagtgatt gttcatgatt tttgatttat
      421 tcaaaaggtt cgcctagaga atctgtctct gtaacgaggc ctggtggaga aactcgataa
      481 aaacctatac tgccagaact gtcctatttt cggatagaca aactagtagt tttaaaaaga
      541 tcatccgatc caatcatttc aaaaaatgca aaactgatat caggatcatt tagctcaggc
      601 ttgttatcgt aaagcatttg taaaggttgc atttagttag gagtaaacgc gaaactatca
      661 tcattatttg ctgaagaaga tgaagaagct aattcagagt ttgtttgaaa taaaggagac
      721 gtgctgacag tttctttacc ctgttttttg cagaggtaga aacttttata tcggaagttt
      781 ctaccggaga atttttctca tttgtagaaa ttgttgaatc gatactattc gaaatttgcg
      841 tatctgaagg tatgttcttt gatgcagaag tcccacaagc agtaagtaac aataaatttg
      901 ataaactgat aaacagaatt cttttcatta tgtacacact ccttatagta tcgtttagca
      961 tacacctatg ctcagtatta ggcaaaatca at
//
LOCUS       DAZUKR010000291          986 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000291 DAZUKR010000000
VERSION     DAZUKR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.304"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACG89C_002924"
     CDS             <1..704
                     /locus_tag="ACG89C_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4901392.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAZUKR010000292          946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000292 DAZUKR010000000
VERSION     DAZUKR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.305"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACG89C_002925"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACG89C_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4901393.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          266 a          202 c          164 g          314 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtccca
//
LOCUS       DAZUKR010000293          931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000293 DAZUKR010000000
VERSION     DAZUKR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.306"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACG89C_002926"
     CDS             <1..>931
                     /locus_tag="ACG89C_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4901394.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAZUKR010000294          925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000294 DAZUKR010000000
VERSION     DAZUKR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.307"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..355
                     /locus_tag="ACG89C_002927"
     CDS             86..355
                     /locus_tag="ACG89C_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4901395.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="ACG89C_002928"
     CDS             348..605
                     /locus_tag="ACG89C_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4901396.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          355 a          100 c          174 g          296 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt
      841 gtagtgtggg atgttagtta ctaaaggatg acgcttatat atatgactga atagaataag
      901 caataggttt aataatctat tttaa
//
LOCUS       DAZUKR010000295          904 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000295 DAZUKR010000000
VERSION     DAZUKR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.308"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACG89C_002929"
     CDS             265..888
                     /locus_tag="ACG89C_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4901397.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAZUKR010000296          889 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000296 DAZUKR010000000
VERSION     DAZUKR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.309"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>889
                     /locus_tag="ACG89C_002930"
     CDS             <1..>889
                     /locus_tag="ACG89C_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901398.1"
                     /translation="KIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINRALSL
                     ESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLF
                     ICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKL
                     YDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKA
                     AIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGIL"
BASE COUNT          283 a          118 c          125 g          363 t
ORIGIN      
        1 aaaaattttt tctgtaaaaa tatttatagt ttatgcattt gttagtttat tatatttatt
       61 tccagcattt aaaaacatgg aaatatttgg cttttatgat acggcatttc acattaatcg
      121 tgcgttaagt ttagaatcta tttttagctc ccctattaat tttgaaacgt ttagatcata
      181 tggcatgcaa gtaaataatt tttacccatg gttaactcta tatcctttat ttttattgat
      241 caagtttact aatttagcaa taggctataa cctttttctt tatattgtta cgctcattac
      301 tctatttata tgtcattatg taatgtatga aattaccaaa aaacatgtta ctagctcctt
      361 tttcgctatt atatatacaa catcttcttt taggagtgta gaaatatttc ttcgaggggc
      421 aatgggggaa ttactcgcaa tgtcaatact tcctcttatt ttactcgggt ttataaagct
      481 ttacgactca aaaaaagaat cttgggttat gttggcgatt agcatgactt tattaattta
      541 cactcatgtt ctttctgtag ctatgactat ggtaatgatt ataattgctt taattattca
      601 attttatcgg aaaaagaaaa tagaaatctt cttaattatt aaattaataa aagctgctat
      661 agttacttta cttctttcac tagcgtttat aggtcctatg attgagcaga cattgtatca
      721 agatctgaat agaccttatg tggatatatt acaaaaaaga gcaatttatc tgggtaaaga
      781 gtttttgatt ggaagtatag attcagaatt actatcattt ggtattggct taactttgct
      841 tatttgttta attatactaa catttaactt taaaaaatta ggaatctta
//
LOCUS       DAZUKR010000297          867 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000297 DAZUKR010000000
VERSION     DAZUKR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.310"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..554
                     /locus_tag="ACG89C_002931"
     CDS             <1..554
                     /locus_tag="ACG89C_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4901399.1"
                     /translation="LDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLE
                     NDKNQLVSEKTNLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQN
                     EKNKFNEEQSTINSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQ
                     KAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          387 a          108 c          120 g          252 t
ORIGIN      
        1 tattagacac agctctagaa aataaagagc aaaaaattag tattttaaat tcacaattgt
       61 cttcaaacaa ccaaactctt gaacaactaa gaactaatat tgaagtttcc aaacaaaaag
      121 tggcatcttt agaaaacgac aagaatcaat tagtatcaga gaaaactaat ttagaaaatc
      181 aattgtcagc aaaagactct gaattgcaag gaaaacaaaa tgaaattaat gcgaaaatag
      241 acgaaattaa ccaaaaagta gctgaaataa atcgattaaa tagcgattgg aataatcgtt
      301 tacaaaatga aaagaacaaa tttaatgaag aacaatctac tattaatagt ttaaaccagc
      361 aaattaatca aatgttgagt gaaaaaaata acattcaaaa tcaactgcaa gctactcagc
      421 aaaagctaga agaagaaaag tcaaaaaata gtagtttgaa taattacatt gaaaagcttc
      481 aaaaagctaa aaatgatgta gaggataccg caaataaagc acaacaaatt gtaaatgaac
      541 atacaatcca atagatatag ttttaattta acaaatggta tgtttggaga taatgagata
      601 gaatataaat gtcataatat tatacaatct aaacaccgtt tccttttcta tttttgatga
      661 catcagtata tagctactcg aaagagtagt ctttttatta ttctcgctta ttattgatat
      721 gaaaatttcg atttgacaaa aggtaccgaa taattttgta atatgaatta ttatcaagaa
      781 gtaattatga tgataacata tggaaactag ttagtaataa taacaggtga ttaaactttt
      841 gaacgacttg taattctata ttttttc
//
LOCUS       DAZUKR010000298          860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000298 DAZUKR010000000
VERSION     DAZUKR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.311"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>860)
                     /locus_tag="ACG89C_002932"
     CDS             complement(<1..>860)
                     /locus_tag="ACG89C_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4901400.1"
                     /translation="YHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLI
                     SVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSI
                     REVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLP
                     EKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLD
                     TTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFL
                     GIVLIFILSI"
BASE COUNT          328 a          146 c          108 g          278 t
ORIGIN      
        1 aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt tacttctaaa
       61 aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac atataaaagc
      121 aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc catgtgctct
      181 ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt ttgcatcgct
      241 attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta taactttaag
      301 cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac tagaatgctg
      361 taatattact tctttttctg gaagtggagt tttatctgat tcatttgact ctataacttc
      421 tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat aattagtacc
      481 aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga tttttacagg
      541 tagtacttct ctaatgctct ttctaaattt tgaattggca aacaacaaaa gaaataatac
      601 taaaaaagaa caaattacta cctgggcacc tctagacgaa gaaagtgtaa tataaaatat
      661 ttgtacgata aaattcatca tataaaaaac acgaatcgtt tttgttattt ttgaaattaa
      721 tacactaatt aaacttaaaa taatagaagt atatccaaac atagctccaa catttggact
      781 tgtatagaca ccaaataacc gatgttcaat aaaaccttgt cttgcaagta atcctggcgt
      841 tcctgttggg acatgataag
//
LOCUS       DAZUKR010000299          832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000299 DAZUKR010000000
VERSION     DAZUKR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.312"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /locus_tag="ACG89C_002933"
     CDS             complement(<1..302)
                     /locus_tag="ACG89C_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901401.1"
                     /translation="MCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQLGVN
                     CFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVKEI"
     gene            complement(449..>832)
                     /locus_tag="ACG89C_002934"
     CDS             complement(449..>832)
                     /locus_tag="ACG89C_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HFS4901402.1"
                     /translation="SNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVV
                     RDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMR
                     DELSELAKKVKVTFLTDPRWVRIDK"
BASE COUNT          287 a          169 c           74 g          302 t
ORIGIN      
        1 aatatttctt ttacaaagct gttcaatgaa aatgtttcta ttccacatgc acaaagaatt
       61 ccataaacta atacactata aaaaatacat tctaatacaa ttcccaaaac attttcaaag
      121 tgaatgtttc ttttctcact taaaaaatat gccgaaataa atataaaaca attaactcct
      181 agctgacccc aaataccaat ataattcaag aaaatctggt tagcagttaa tgaaccatgt
      241 actataattc cgtctgcatt ataatttaaa cgcaaattag catggtaagc aacaatacac
      301 aatatagaaa tgattcttaa aagttcaaaa ttagagtttc atctcgcccc atcgtccctt
      361 aaatattttt cttctttttt atttaagact ttacccattt ttattctctc actatctctt
      421 tatcaaaatt ttaaaaacat tattactact acttatcaat tcgaacccaa cgtggatccg
      481 ttaaaaatgt cacctttact tttttagcta attctgacaa ttcatctctc atttgcacgt
      541 tcattgcatc taataatgca aattttgtta taatttccat ggattgctca ccataattct
      601 catcttcatt atgtgaatat ccatcaaatc cagctaagaa tatttcatcc acattcatat
      661 cggctaaaaa tctaattgcc attaatcctg cattgtctct tacaacttcc ctttcagata
      721 acaacttttt ataactcgtc cgtaaatata catccgatgc ttgtatattt gatgtaacaa
      781 tacatttagt tctgctttct ttcggtaatt catgaaaacg tcttaaatta ct
//
LOCUS       DAZUKR010000300          828 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000300 DAZUKR010000000
VERSION     DAZUKR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.313"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..656
                     /locus_tag="ACG89C_002935"
     CDS             117..656
                     /locus_tag="ACG89C_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901403.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          318 a          105 c          152 g          253 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaatttt
//
LOCUS       DAZUKR010000301          825 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000301 DAZUKR010000000
VERSION     DAZUKR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.314"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..157
                     /locus_tag="ACG89C_002936"
     CDS             <1..157
                     /locus_tag="ACG89C_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFS4901404.1"
                     /translation="RTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQR
                     LFGDENE"
     gene            331..672
                     /locus_tag="ACG89C_002937"
     CDS             331..672
                     /locus_tag="ACG89C_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901405.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            665..>825
                     /locus_tag="ACG89C_002938"
     CDS             665..>825
                     /locus_tag="ACG89C_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4901406.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINE"
BASE COUNT          315 a          131 c          166 g          213 t
ORIGIN      
        1 acgtaccttt tcaatcccaa gatatggcga gttacctcgt aagagattcg atgatgctgt
       61 ttcatttatt gaaatgtggt taccagaaac tgcgatccgc atggaaatcg atcaactgaa
      121 cagacaacaa agacttttcg gtgatgaaaa tgaatagagc tgaagcgcta agaataggga
      181 cggtaattgc taatcgctgg tggagacaca ataaaccaaa catcctaagc caacaacata
      241 tcgaaaagca aaaagcatgg caacaaataa aaaagtgact cagccggcaa gcatagagtc
      301 acaaaacata tctaaggaga attttagcat atgaataaag aactttccac tttagatcaa
      361 tatttgactg attctgaatg gggcaagtcg aatatcaagg aaacaaataa tcgaaaaatc
      421 agacgaaatc ttttgacgaa cgaagaacta tcatgtgatc aagatgactt gggaaatttt
      481 gtgagtattt gggatcatgt ttaccttatc catctatcaa aacattcaaa taaacctgaa
      541 tacatctatg tcatcgaaga tggcttgatt gatgcgctag aagagtacaa cagagataac
      601 ttgattgata tctcttatta cggatcaggt aagaaataca ttgctgaaat ggaggcagaa
      661 tttgatgagt gaaatcaaag ggacaacgaa ctttgaaaaa ctttttagtc gtaagttaaa
      721 taaaattctc aagaaaaaag gaaattttga ttatttatct tgggctcacg cgtgggagat
      781 tatgaaaaag aatgatccac aggcaacggt aactattaat gagta
//
LOCUS       DAZUKR010000302          825 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000302 DAZUKR010000000
VERSION     DAZUKR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.315"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..377)
                     /locus_tag="ACG89C_002939"
                     /pseudo
     CDS             complement(1..377)
                     /locus_tag="ACG89C_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(436..>825)
                     /locus_tag="ACG89C_002940"
     CDS             complement(436..>825)
                     /locus_tag="ACG89C_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4901407.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          242 a          146 c          139 g          298 t
ORIGIN      
        1 tttatgactg gataactgac caattcatga cgatccaagt cttctttatt catatttaca
       61 gcagtaattt gactgtcttc atacgtacga taataataaa tccctttatc aacgttacag
      121 caagaagaat aaatcgtata ttcgtatttt ccttctccag tatcgcatac tcctttttgt
      181 tgctcgactg agcctagtat atgaaaaaat tgactgatac tttcagattc tgaatcacct
      241 gatgcagcat tcattttagt aaatgtggct tttacaaatc gagaaacaga agagagatct
      301 ccaggtaagc ccaatcctcc catcccacga ctataaacat tcaagctgat ctggtttgaa
      361 aaatgatttt ctggtgttcc attgggagcg tcaataattt tgtgtaaata aattgtcctc
      421 ctgcaaaata attagttact cagtaaacat tgaaactaat gtatcggtta cctgttgaaa
      481 acctttatgg cttctgttta gaaatttttg attgtatgta tcaaaaatgc tgactagaaa
      541 gcgttctagt gattcttcat tttgaaactg ctcttttcta cggctgtatc ttttaatttg
      601 cttattgaaa gactcgatta gattggttga gtaaatggtt ctacgaatgc taggtggaaa
      661 atcataaaaa gttaataagt cttggttttc tatgagtgac tgcgtcactt taggatagtt
      721 tttcttccat ttctcaatca tgccggataa gaaggtattc gcttcttctt ttgagttagc
      781 ttgataaaca gccttaaagt catcacagat ttcttttcgg tcttt
//
LOCUS       DAZUKR010000303          811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000303 DAZUKR010000000
VERSION     DAZUKR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.316"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG89C_002941"
     CDS             <1..672
                     /locus_tag="ACG89C_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4901408.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZUKR010000304          785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000304 DAZUKR010000000
VERSION     DAZUKR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.317"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..619
                     /locus_tag="ACG89C_002942"
     CDS             50..619
                     /locus_tag="ACG89C_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS4901409.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          248 a          101 c          160 g          276 t
ORIGIN      
        1 tagaacagat ttttaatgac ataacgaaat agaattgagg agaatatata tgaaaacaaa
       61 agattatatt tttattggtt tagctacagt tatttcccta gtaatttact ttttttcaat
      121 aatggttagt agtattggtg gggcgtttgg tcacagcata tcccccggga tttttggatt
      181 attatctgga atcatatttg tttatattag ctataactac cctagaaaag gaatttttac
      241 tatttacaca attgttttat tgttattttt tacgttaatg ggcggggcgt acttaccttg
      301 gtttatcagt tcgattagta tggcgatctt agctgatatt attctatctg tatttggata
      361 tgatcgagca attcctcaag ttgcgtcttg ggctctaatg caattaggca gtgctgccgg
      421 acagtggatt cctatatggt tctttacaga tcgttttcgt caagattgga tagataaagg
      481 acaatctgct gcaacaatgg atgcaatgat ccactatgcg gtaggtatat ggggaatcat
      541 ttcagtgtta gttgtagcaa gtttatctat gattggtgta ttgattggaa gaaaagtctt
      601 aaaaaaatac aaaaaataga acgtagttta ccttttatct gtttgtacat ctctaattta
      661 cgctattacg gaaagcatta acagttgtaa acgaataaga gaagaaattg aatgcggggg
      721 aggaaaaaat gaaagtatac aaaaaattat ttagttatgt ccctaaaatg aaatactacg
      781 gatgg
//
LOCUS       DAZUKR010000305          779 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000305 DAZUKR010000000
VERSION     DAZUKR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.318"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..282
                     /locus_tag="ACG89C_002943"
     CDS             <1..282
                     /locus_tag="ACG89C_002943"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4901410.1"
                     /translation="SDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDV
                     SDLKIRLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            312..>779
                     /locus_tag="ACG89C_002944"
     CDS             312..>779
                     /locus_tag="ACG89C_002944"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4901411.1"
                     /translation="MHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLG
                     GKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRI
                     AHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKFTLIPNA"
BASE COUNT          283 a           85 c          159 g          252 t
ORIGIN      
        1 agtgatttag aaggaatgcc attaagtttg ttagaagcaa tgagttatgg gaactgttgt
       61 atagtttcaa atatctcaga gtgtacggaa gtagtagaag acaaggctat gatttttaag
      121 aaatcggatg taagtgattt aaagataaga ttagaagagg catgcaatca atcggaaatg
      181 gttaaagttt tgaagaatca agctactgag tttatttgta gtaaatataa ttgggataaa
      241 atcgtacaag aaacattaaa tttgtatagg ggaaatatat aatgattcga atattacagt
      301 gtgtgaatga tatgcatcgt gcagggttgg agacgatgct aatgaactat tataggaata
      361 tagatcgaac taaaattcag tttgattttt tgacacatcg tccatataaa agtgattatg
      421 atgaggaaat aataagtctt ggaggaaagg tctattatgc accgagactt tatccacaaa
      481 attatcctaa atattttaaa tggatggaac aattttttaa ggatcatcca gaatatgaga
      541 ttgttcattc tcatattgat gcgatgagtt atttaccttt aaaagctgca aaaaaagctg
      601 ggattccgat acggatagca catagccata atacatcgat agatagagat tttaaatatc
      661 ttttaaagca atattatcga tgtaagttaa cgtctgtttt gacacatgaa ttttcatgtg
      721 gaaaagtagc aggtgatttt ttgtttcgaa atgataaatt cactttgatt ccaaatgca
//
LOCUS       DAZUKR010000306          753 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000306 DAZUKR010000000
VERSION     DAZUKR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.319"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            542..>753
                     /locus_tag="ACG89C_002945"
     CDS             542..>753
                     /locus_tag="ACG89C_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4901412.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVED"
BASE COUNT          277 a          117 c          104 g          255 t
ORIGIN      
        1 caccgagaac tcatcatttt tcatcatgat gaatactatt tacatatatc tagttcaaag
       61 acaataatta ttatgatccg ttattatcta agcactatac agataatagg aatttttcgt
      121 ttctaaatat tccaatttgg gaatccaata aaaattttcg ttcaaatacg tgtctattcg
      181 aactcttttc tagttgttaa ttatgaaggg ggaagaaata aaaaatacaa tctatacacg
      241 aataagaata ttatccaaaa ggaggtttta gaaaagattt attatacgta aaaagctcaa
      301 gacattttct tagccataag gacatgtttg atatttaaga agatatatga attttctttt
      361 ctaagtataa agataactat ccattatcaa ctaaaaaaac atttttgaaa ataaacatca
      421 gcagtcctat caatgcatgt gagcttatag aagttattcg cagcgcagca taattgcatt
      481 agcttctttt cgtgtttttt gcctcttttc cctttaaatc ttaatggagg aggtgtgcaa
      541 tatgtcagat aacgacgaac gcgtaaatgc ccagttgatt cgattctttg aaaaggtcat
      601 tttgaacacc gctaatactt attataaaaa acaagaagaa attcaaaaac atgaacaact
      661 tgatcaactc ccaccatatt tgtttactaa agatactata aaatttaaac aaccgattaa
      721 tgttttaaat gtgactctcc ttgttgaaga tat
//
LOCUS       DAZUKR010000307          736 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000307 DAZUKR010000000
VERSION     DAZUKR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.320"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..>736)
                     /locus_tag="ACG89C_002946"
     CDS             complement(101..>736)
                     /locus_tag="ACG89C_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFS4901413.1"
                     /translation="RGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTD
                     LTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTD
                     RGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQL
                     RLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          203 a          169 c          140 g          224 t
ORIGIN      
        1 ttataaggtg ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc
       61 ctcggatctg attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg
      121 tatcagatcc gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga
      181 tcggtgtttc atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat
      241 caaacaattc tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta
      301 ctttgacaga tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac
      361 tcaatgaacg agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt
      421 cctttccccg gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa
      481 aggcttcttt taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc
      541 gattaaacaa atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag
      601 taagatccgt aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat
      661 tcgctgtttt cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg
      721 tagatgtcaa tccgcg
//
LOCUS       DAZUKR010000308          733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000308 DAZUKR010000000
VERSION     DAZUKR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.321"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACG89C_002947"
     rRNA            <1..>733
                     /locus_tag="ACG89C_002947"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAZUKR010000309          711 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000309 DAZUKR010000000
VERSION     DAZUKR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.322"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>711)
                     /locus_tag="ACG89C_002948"
     CDS             complement(<1..>711)
                     /locus_tag="ACG89C_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901414.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYS"
BASE COUNT          235 a          115 c           86 g          275 t
ORIGIN      
        1 tactgtattt ttttaattcc ataagaaaat tagttattac ctgccaccta atcagcaaat
       61 ctgaacctga gtcaatataa ttaatattat tttccatctc caacattgta agtactgttg
      121 gtgttaatat agggactttg tacaattgta tttttttgat tttatcatct gcacaaagga
      181 gattttttaa atctaatcct tcttttattt caaaagttga attttttaaa tctataagag
      241 taaaaccaat tccttcatac tgaatttctg aaaatcgaat ttttctccca gttgatagat
      301 caaggggcag aaaacctttt attacattgt gagtgtcacc atcatttaca atgtcattag
      361 gaattttgat tttagaattc tgtggagcac ctattggaag ataataattc attataattt
      421 tgtagtcttc atgaggaaat aaagtacttt taggattgct aagatattct cgtaccgaat
      481 ctaagaatgg cttatccatt gaatcttcat ccccatttaa actaaaaaac attcctacaa
      541 ttgcttttgc aactctaagt gaattcattt tctttattga atgtgttaaa tcagacttac
      601 cattctttaa acttgtattg ttataaagga aatttttatt gtcttctgca aatctaatat
      661 attcctcaac atactcacta ccaaaaaaac tattacattt actacataac g
//
LOCUS       DAZUKR010000310          693 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000310 DAZUKR010000000
VERSION     DAZUKR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.323"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          254 a          119 c           93 g          227 t
ORIGIN      
        1 tccatataga tttttaaatt acacactagt ttaaactaaa acaaacaagg aaaaacttac
       61 catttaatca aaaaattagc tcatatgaat atattagaat gtaaattaga ctggtcaaaa
      121 tctggtcaaa aaagaggacg aatacaggta gtcctctttt ttgttatttt gatattctaa
      181 atcatttttt attggattat tcccttgttc gatatagacc caaattgatc tatcaataat
      241 tatcaaactg agtcatatag agacaaaaat ttcaaaaatg acctgttaaa tggtcatcga
      301 atatcattta aaatcaatac gacggagtca gacacggagt caaaacaagc ccgctgagag
      361 taaaaaatat acagtcccat aggtaaagtg ccacaggact gtatattttt ttatttgaga
      421 ttacctgaaa tgattccatt taatttttat ctcatttttt cctactagtt agtatcaaat
      481 actctcctat tttctcaata aactattttg ggttacatgg tatctgaaac agctaaaacc
      541 ttgtcattat agcaaaaaat cactttatct ccgtttcatt tcagatacat ttcacttaag
      601 cggattccaa aaaaacaaaa gaaaaaggaa cccctagtta gcctatgctt tttattggtt
      661 cctctcaaaa cacggtcata taacaacggg gtg
//
LOCUS       DAZUKR010000311          689 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000311 DAZUKR010000000
VERSION     DAZUKR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.324"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          271 a           73 c          125 g          220 t
ORIGIN      
        1 aattttttcg ttagtgtatt aatgggtgaa agtttaatcc taatactgat tttgtgttta
       61 attgttcatg aagtgaataa acaattgtga attaaaaaaa taaaaaccta gtataatcac
      121 tttaataaat tgttggtaga aggtgatcaa aatgggagta aacgaaatga tgaaagtcag
      181 attgagtttt taaatgatac cttcctcaga acttcaaaac gaattcaaag attcgttgac
      241 aaatcttgag caagatcatt taaaaaaata ttttctcttc atttgacgaa aaatgtttag
      301 aaatcttata taagtgaaga taaagtaatt ggtgtaatta attgtagaga atagcctttg
      361 agcaattgaa gcgatacaat tatttagaaa atgaacttcc tccagcatac tacatgattc
      421 cttacgggac gatgttaaag aagaaagcaa gaaattttag aagtattagt agataaacta
      481 atgggctttt ctcaatttaa gggagtagcc caaatgttta tcaaatatgg tgcagataca
      541 gtagcaacca tgtttacaga agaatatcaa gtagactatt atgaagtaat agataaatgt
      601 gtacattttg tttatgaaaa agtgtggaat tttatgaatt ttgcgataag tgaagaaatc
      661 ttacgagtac aagaaaacag aacaggaat
//
LOCUS       DAZUKR010000312          677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000312 DAZUKR010000000
VERSION     DAZUKR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.325"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..538
                     /locus_tag="ACG89C_002949"
     CDS             50..538
                     /locus_tag="ACG89C_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4901415.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          270 a           57 c          118 g          232 t
ORIGIN      
        1 gaaaacatga gcaatagaat tgtactagta ggataaggat ggatggaaaa tgaaaaaaac
       61 tatttttgtt ttagcgattt ttatatgttt tgggctactt ttggtaggct acttgttgtt
      121 taattctaat gaaatgagaa ctatttctac gaatgacttg ttaaaaaaaa gtgaattaaa
      181 ggataaaaaa aaagaatctc ttgcctttat aatgtttact caagaagagt gtgagtattg
      241 tatagcaata aatccaatca tagatagtgt aaataaggaa actttgaata agataaagaa
      301 atatgatata tctgtagatt ttcaatctaa acaaaagtgt aaaattaagt ttgttccgac
      361 tattgttgca tatagtaaga ataaagaagt ggggagaatt gacaatgaat tagataattt
      421 gtatcgaaaa aagcaagatg gttcaattgt aatagacaag aaaaaaacaa agacattgct
      481 tatagattgg atgaaaaaaa tggataaaca aggagaatat ttgtatggag aagattaaac
      541 agacatttcc gttaattaat aaatattgga ataagaaaaa aatttttata ttttttatta
      601 cgtttctgct gtcatcttta ttttctctaa ttttcccgta tctatttagt aatgttattg
      661 atttatattt agagaat
//
LOCUS       DAZUKR010000313          673 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000313 DAZUKR010000000
VERSION     DAZUKR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.326"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>673
                     /locus_tag="ACG89C_002950"
     CDS             <1..>673
                     /locus_tag="ACG89C_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFS4901416.1"
                     /translation="MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPE
                     THCLIVMHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTN
                     GLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDF
                     DSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKI
                     AKEKDT"
BASE COUNT          251 a           76 c          132 g          214 t
ORIGIN      
        1 tatgaaaaat gataaacaaa aattattggc aaatgagatt tggagtagtt tagaagtcga
       61 agaaagtaaa caagaacttt tgttgaaatg gatacttttt cttgtaaaga agttctatgt
      121 gaaaatacca gaaacccatt gtctgatagt tatgcacggt aaaataacag cttctgcgat
      181 cgcaagggaa acaaataagt tgctgaatac atatgtttat gaggctttcg atatgccaat
      241 tgaaggtgag actagtgact taattaacct aatcaacaat ttttgtaaaa gtatagatac
      301 gacgaatgga ctcattctgc tagttgatat gggatcattg gaacaaatgt atgaaaaaat
      361 tgaatccaat gtaataggtg atttggtaat tttaaacaat gtttctacag ctttagcgat
      421 tgaatgtggg attcaagttt gccaaaaaaa gccaattagt catttttacc agatggattt
      481 tgattcgttt caagttaaag ttcagtatta taaaggatta tcacaaaaga aaaatgtaat
      541 agtatcttgt ctctctggtg aggggatttc tgaaaaagtt aaggatattt taaaacggta
      601 tctcgataat caagtggaga ttttgatttt tgattttaac gcattaaaga aaatagcaaa
      661 ggaaaaagat act
//
LOCUS       DAZUKR010000314          652 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000314 DAZUKR010000000
VERSION     DAZUKR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.327"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..447
                     /locus_tag="ACG89C_002951"
                     /pseudo
     CDS             112..447
                     /locus_tag="ACG89C_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            543..>652
                     /locus_tag="ACG89C_002952"
     CDS             543..>652
                     /locus_tag="ACG89C_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4901417.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          230 a          127 c          132 g          163 t
ORIGIN      
        1 aaaatgcaat acttttcgag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
       61 ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca tatgaacgat
      121 tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt
      181 gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc tttcctcgat
      241 tacgaaaagt acgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac
      301 cgtacggtca agaccgagta cggggaactt catctccaga ttccgcgcga ccgcaacggc
      361 gagttcaagc aacagactgt tcctgcttat agacggacga atgatacctt agaggagacc
      421 gtcattcacc tcttccgaaa aggtatggat aggctacaat taactagtgg ataggctaca
      481 attaactaga cagaaaaatt aaggtgtgta gactagaaga aaacatacca ggaggaattt
      541 ttatgtctaa gagaacacga agaacttttt cacaagaatt caagcaacaa atcgtcaatc
      601 tttacttagc tggaaagcca cgtgtagaaa tcattcgaga atatgaacta ac
//
LOCUS       DAZUKR010000315          634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000315 DAZUKR010000000
VERSION     DAZUKR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.328"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..623
                     /locus_tag="ACG89C_002953"
     CDS             252..623
                     /locus_tag="ACG89C_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4901418.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT          234 a           88 c          126 g          186 t
ORIGIN      
        1 tttccttgac tttttgaaat aattcttctt gattcatttt gaacacatcc tttgctatca
       61 acatgatttt ttacatggac aaaacgagaa aaactgattt atccacacgt caataatatc
      121 aaataacgat catttttact aaaaaaacac tcgattctcg cttctaaagt aggagaaaaa
      181 tctccaactt gggacggatg gaatttcttt aactgaccca tatactgaat atataacaag
      241 gaggtagaaa gatgaaagtg attaaatatt tgattggact ggcggcgatt agtggtattt
      301 tgctatttgg actgaaatat tatactgaag gaagttatgg agaaatccct ggtatacttg
      361 atcagctaaa tccattagtt gaaaaaggag aggtctatgt aaagacacaa aaaccagagg
      421 aagtaaatga atatggtaca gcaaggtatg tgcagaaagc tgctgatgcc aatgggaaag
      481 aacgaacggt tgaatttaat ggtctttctg tgttaaaaga aaatcattat ttgaaaatta
      541 gcaataaagg agcacacata gaaacgtatg aggaagttcc tagagaaaaa gttccggaga
      601 aagcattagc aattattggg taaatcagtt tttg
//
LOCUS       DAZUKR010000316          626 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000316 DAZUKR010000000
VERSION     DAZUKR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.329"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..626)
                     /locus_tag="ACG89C_002954"
                     /pseudo
     CDS             complement(1..626)
                     /locus_tag="ACG89C_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          215 a           98 c           94 g          219 t
ORIGIN      
        1 attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat atttttaata
       61 gaaaatataa tttatctatc ataccgctat ttagtcggtc aagtacttct tttttaaaaa
      121 aagtattcat cataaaagcc actacttgcg gatcttcatc ataaaattct gtagcagtac
      181 ttgaaaagat gttccaataa atatctaatg ctgcatattg aatttgtacc tcttttccag
      241 aaaaagactt tctgccatta ttagaaaagg agatagacaa accgtactat gctaaaaatg
      301 cattaatctt tttcattttc gtatacaaat aagaggcact atagtttgtt tgctgactga
      361 aactgataat cgaatttgtc tgacagctga acaaagaagt caagatctga taactgactg
      421 attcgttgca gtatcttcca aatagacgat aatagcagta agccggatca attccttcaa
      481 aattcaactt taaaaaaggt ttttgcttat caatatgcag ttctgttaga ttgagacgct
      541 caaagtcttg ttctaattct ttgatgtata gtaaaaaatt tgattcagac atatggaaat
      601 gatctgctaa atattgtgta gtaata
//
LOCUS       DAZUKR010000317          602 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000317 DAZUKR010000000
VERSION     DAZUKR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.330"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..535)
                     /gene="spxA"
                     /locus_tag="ACG89C_002955"
     CDS             complement(137..535)
                     /gene="spxA"
                     /locus_tag="ACG89C_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS4901419.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          180 a          124 c           97 g          201 t
ORIGIN      
        1 gcaaaacatt tttaaccaaa taaacagaac agaaagaggt tttcttcttt ctgttctgtc
       61 caaattattc taagatttct ctttaccaac atcatttgac aaagagccag tcttttccaa
      121 gtttcgtatt atccttctat aatccagcca tcaattgtgc ttgacgtaat tctaattgtc
      181 gtacatcgcg cggtaggaac cgacgaattt catcttcatt aaaaccaact tgcagacgtt
      241 tatcatcaat catgattgga cggcgaagca atccaggatt gttttgcaca agttccaata
      301 attcttgtaa cggcagatca tctaaatcca tgttcagttt ttggaatact ttggaacgtg
      361 tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa aatcgctttt aattcagaac
      421 tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg aatctgatgt tcttgcaacc
      481 acgcacgagc ctttctgcag gaagtacaac ttggggaagt atataatgtt aacaacgtgc
      541 accactcctt ttcgtgataa gaatcacttt tttgtaacag aagatcataa taatctccca
      601 tc
//
LOCUS       DAZUKR010000318          595 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000318 DAZUKR010000000
VERSION     DAZUKR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.331"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..521
                     /gene="scm"
                     /locus_tag="ACG89C_002956"
     CDS             <1..521
                     /gene="scm"
                     /locus_tag="ACG89C_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4901420.1"
                     /translation="ASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDST
                     DTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTR
                     HASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGL
                     RITFMSYKKSKR"
BASE COUNT          220 a          133 c          101 g          141 t
ORIGIN      
        1 cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagcacg gacacgtcag
       61 cttcaagcga cagtacggat acatctacca caacaagtga tagcacggac acgtcagctt
      121 caagtgacag tacagatact acctctacca caagtgatag ctcagattca tctaccacta
      181 caagtgatag cacggacacg tcagcttcaa gtgacagtac agatacatcc tctaccacaa
      241 gtgatagctc agattcatca acttcaagcg atagtacaga cacgtcgtct accacaagcg
      301 atacacgtca tgcaagtgtc tccttctcaa aaacaagttc ggataacagg acgaacttta
      361 acttaactaa taaaacaagc aatgacaatg gaaacaaatc aaaaagagca cttcctaaaa
      421 ctggaagtca atcgaataat tggataacac tcgcaggcgt tatattactt gtaatcggtt
      481 taagaatcac ttttatgagt tataagaaat cgaaaaggta aaaaaatatt caagataaaa
      541 aagagcaacg tcgctaacgg acgttgctct ttatttttgc tttttatcat aaata
//
LOCUS       DAZUKR010000319          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000319 DAZUKR010000000
VERSION     DAZUKR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.332"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG89C_002957"
     tRNA            complement(65..150)
                     /locus_tag="ACG89C_002957"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG89C_002958"
     tRNA            complement(167..238)
                     /locus_tag="ACG89C_002958"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG89C_002959"
     tRNA            complement(250..322)
                     /locus_tag="ACG89C_002959"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG89C_002960"
     tRNA            complement(340..421)
                     /locus_tag="ACG89C_002960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG89C_002961"
     tRNA            complement(429..501)
                     /locus_tag="ACG89C_002961"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG89C_002962"
     tRNA            complement(505..577)
                     /locus_tag="ACG89C_002962"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZUKR010000320          586 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000320 DAZUKR010000000
VERSION     DAZUKR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.334"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..>586
                     /locus_tag="ACG89C_002963"
     CDS             330..>586
                     /locus_tag="ACG89C_002963"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4901421.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDK"
BASE COUNT          214 a           63 c           99 g          210 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaa
//
LOCUS       DAZUKR010000321          533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000321 DAZUKR010000000
VERSION     DAZUKR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.335"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..466)
                     /locus_tag="ACG89C_002964"
     CDS             complement(275..466)
                     /locus_tag="ACG89C_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901422.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          163 a          110 c           76 g          184 t
ORIGIN      
        1 gaaccggggg ctttattcct gcagtccggc tacgccttcc tgcaatagac aaaactccag
       61 atcgggtaat tcgcttgtgg cgaaacccga cctggagttt gtctctgtat tttgaaccct
      121 aaaaattaat caaacagact gatattctca taatcagcgc gaaataagcc attaagtaca
      181 gttttcctac ctatttttga taacccgctg gttagttaat attccttttc cgagagagat
      241 aaatgattgg ttaaatctat atacatctca acctttaaaa atgttttcga aatacttcat
      301 cagtacatgt catacaaatt accagtgcat tctcaaattc tagttcttta attgatttca
      361 gttgaggagc agttactttt ggcggaaatt tccatttttg tccaaacctc ttcccccgat
      421 gaaatttctt tgttgcaatt attgcaatga aaaatttttg tttcaaaaat atacataatt
      481 acttcctcca ataaaaacta tctacctctc aaattatttt tctctaataa gaa
//
LOCUS       DAZUKR010000322          527 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000322 DAZUKR010000000
VERSION     DAZUKR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.336"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..497)
                     /locus_tag="ACG89C_002965"
                     /pseudo
     CDS             complement(132..497)
                     /locus_tag="ACG89C_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          156 a           99 c           79 g          193 t
ORIGIN      
        1 attttagcat actcagtttt agaaaaagca aaaaaataaa gaaacttatt cttgataaca
       61 agctacgtga cgtatatccc taacacaaaa atagccatat cctttggttt ggatacggct
      121 atttgatatt actcattcaa ttatcaaatc acagtaggtg tgaatctcat cgggatcggc
      181 aatatctact tctgattagg atcaaaggtg attttctcaa taaaggtgtg atacaaagct
      241 ttaacttgct tcttctcact ctggataaga aagcaatcca aactagtcaa tatctgttgg
      301 acatctttga cttgaatcgg ttccccttgt tgttgaaaga tccctaagat ttcattctct
      361 cgaatttgta actgtcttcg tttttcagtc aattcattta aacgatcttt taacatcgga
      421 aacaattcgg gactatcttc caaagcagct tcctgttttt gctctaatag ttagattttg
      481 cgtaccattt tcttctttta ttttccttcg attgctgatt ttaatat
//
LOCUS       DAZUKR010000323          526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000323 DAZUKR010000000
VERSION     DAZUKR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.337"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG89C_002966"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG89C_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4901423.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAZUKR010000324          485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000324 DAZUKR010000000
VERSION     DAZUKR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.338"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..259)
                     /locus_tag="ACG89C_002967"
     CDS             complement(62..259)
                     /locus_tag="ACG89C_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4901424.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAZUKR010000325          477 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000325 DAZUKR010000000
VERSION     DAZUKR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.339"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..362)
                     /locus_tag="ACG89C_002968"
     CDS             complement(<1..362)
                     /locus_tag="ACG89C_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4901425.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHV"
BASE COUNT          146 a           88 c           69 g          174 t
ORIGIN      
        1 acgtgatgct gtttagcaaa attttctact gagatatatt tttcctgaaa gaaagccaat
       61 accattttca tagataaaga atccgttata taacgagata acagagcttt gctagaacat
      121 tttccattaa tttccagaca tatatccaat tcactgacgg ttactcgcaa atttttctct
      181 agatcgaact tttgtaaatc atgtttcagc ttatcgatcg tttttttcaa gagaaatgtc
      241 gatagcgaca actcttcttg aacattcgat attgactctg ataaatttga gcttgcttct
      301 aaatatttaa gtaatgaata acttctgtga tcttctttgc ttaaaaaaaa ctcataattc
      361 attagtcatc agctctttct acgtatatta ctttccatgc attcaccaac tagctgggga
      421 taaaactgat ttcctggatc cgagtcactg ttgtcagcga ttcttgtttg tataata
//
LOCUS       DAZUKR010000326          464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000326 DAZUKR010000000
VERSION     DAZUKR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.340"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..120
                     /locus_tag="ACG89C_002969"
     CDS             <1..120
                     /locus_tag="ACG89C_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4901426.1"
                     /translation="EKFIFDKTDKEIGKVLGITRQGATNLKHRLYKKIQKMLV"
     gene            214..405
                     /locus_tag="ACG89C_002970"
     CDS             214..405
                     /locus_tag="ACG89C_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901427.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          180 a           62 c           82 g          140 t
ORIGIN      
        1 gaaaaattta tctttgataa aactgataaa gaaattggaa aggttctggg gattacaaga
       61 cagggtgcga caaatttaaa acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa
      121 tatctgcata ataactaatt ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa
      181 tagattataa gtccatggga agaaggtaga tttatgaatt ctttaaaaga agatttcatt
      241 ttagcaaagg ctggaaatga agaagctgtt gaggcaattc tcaaaagatt tagctctttg
      301 attcataagc aatcctggag aactggaaaa tatgatcaag attgttatca agaatgtatg
      361 cttgcaatat atctagctat ctcgaaattc gaaattaaag aataactcaa ctaagggagt
      421 ctgtgacata agtaaaaaga tagtttcaaa tttcgaacaa taaa
//
LOCUS       DAZUKR010000327          460 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000327 DAZUKR010000000
VERSION     DAZUKR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.341"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          150 a           89 c           54 g          167 t
ORIGIN      
        1 gaaagcacaa aaaataaaaa aattattacg ttattaaaaa aacagatgaa agatcaggtt
       61 ttattagatc tcattctttc atcacttgaa gggatttaat gaactacttt ttcctctttg
      121 tcgtttttat atattcgtat tccttcaaac agtaggatag cctctttcaa tgcttgatat
      181 ttgaactgtc cgttacttaa aaatttagat gtgccgccct cttcctagaa catattcttt
      241 tttacttttt cattaattga cagaccaatc gaacgaaact gtaaatttcg ataggataat
      301 gattggtcaa actcattcat tttcaagaca tctcgattca tattcaagat aggaatcttt
      361 ttatcaacat cactttattc gtattttcct ctaccataaa agcttcacgc tccacaaaat
      421 tgttttatac actactattc agcccattca acgaccaatg
//
LOCUS       DAZUKR010000328          415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000328 DAZUKR010000000
VERSION     DAZUKR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.343"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG89C_002971"
     CDS             complement(<1..339)
                     /locus_tag="ACG89C_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901428.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZUKR010000329          401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000329 DAZUKR010000000
VERSION     DAZUKR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.344"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          112 a           98 c           76 g          115 t
ORIGIN      
        1 attataaata actcataaaa aatattttga ctttcaaaat cacttttgct accagaagtt
       61 gaggcgaagt gttttaagca tagttgtacc ctagtttgac cgttaaaaat aaataacgat
      121 cgctagagtg caactatgtt tgtttgccct accgtcagcg tcggcttggc ctctcacatc
      181 cattaccgtc gggattatct ccgttacata cccttacaaa atacgccctg taagcattgc
      241 atgaaaccac cgtgcatttg caccgtattt ccgccctagc cgttcaaatc ggtcgctacc
      301 accattcaga aacacgtgcg gtaatggaag gtttggagga agctatccag accactaacg
      361 attgtcccta tcgtcgtacg tttcactcag atcaaggttg g
//
LOCUS       DAZUKR010000330          350 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000330 DAZUKR010000000
VERSION     DAZUKR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.345"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..350
                     /locus_tag="ACG89C_002972"
                     /pseudo
     CDS             1..350
                     /locus_tag="ACG89C_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          148 a           61 c           65 g           76 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga
//
LOCUS       DAZUKR010000331          346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000331 DAZUKR010000000
VERSION     DAZUKR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.346"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..140
                     /locus_tag="ACG89C_002973"
     CDS             <1..140
                     /locus_tag="ACG89C_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS4901429.1"
                     /translation="VDAATYYGNGLYCNKEKCWVNWGQSWSEGLKRWGDNLFGSFSGG
                     R"
     gene            140..>346
                     /locus_tag="ACG89C_002974"
     CDS             140..>346
                     /locus_tag="ACG89C_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4901430.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNK"
BASE COUNT          132 a           39 c           64 g          111 t
ORIGIN      
        1 acgttgacgc agctacttac tatggaaatg gtctttattg taacaaagaa aaatgttggg
       61 taaattgggg acaatcatgg tcagaagggt taaaacgttg gggagataat ctttttggta
      121 gttttagtgg aggacgataa tgacttcaat aaacattgat cacacaaaaa aagtcataca
      181 tgaattatac aatagtttaa tgacacgacc taatcagacg aaagcattat tagatattac
      241 tgatgtttta tttcaggtat ataaaaaaat tgatacagca aaatatcctg aaattcttgt
      301 aaataaatta gttaattata tttacatcgt gggttttgat aataaa
//
LOCUS       DAZUKR010000332          345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000332 DAZUKR010000000
VERSION     DAZUKR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.347"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACG89C_002975"
     CDS             complement(<1..321)
                     /locus_tag="ACG89C_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4901431.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           63 g          130 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaat
//
LOCUS       DAZUKR010000333          335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000333 DAZUKR010000000
VERSION     DAZUKR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.348"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>335
                     /locus_tag="ACG89C_002976"
     CDS             <1..>335
                     /locus_tag="ACG89C_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HFS4901432.1"
                     /translation="YWGYSLPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDE
                     EKKVSYDKQYIEDLYYKYMETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIK
                     EFVTKEEVV"
BASE COUNT          144 a           39 c           60 g           92 t
ORIGIN      
        1 ttattgggga tattcattac caaactatat ttcagcagtt cataatgctc atcctaatta
       61 tgcaagctat ctgtcatata agaatacttt aacaattgaa gcaatgaatg aaatattcga
      121 aatgatggat gaagaaaaaa aggtatctta tgataaacaa tatatagaag atttatacta
      181 caaatatatg gaaactggga aaatacagga ggaatatatg gaagaactga gaaaatttct
      241 ctcagacaaa aaaattctac tgattgcacc aggaaaaagt tctgtagatg aggtagaaag
      301 aataaaagag tttgttacca aagaagaagt cgtaa
//
LOCUS       DAZUKR010000334          333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000334 DAZUKR010000000
VERSION     DAZUKR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.350"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           56 c           55 g          109 t
ORIGIN      
        1 aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa
       61 tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct cctttcacaa
      121 gtatggtgat aaaattcgtt ccgggctacc tttttaggta gcctacttta atctttatac
      181 ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg
      241 tccggcacgt cagtcatgaa tcggctacac tagactagac agaaaaaata aggtgtgtag
      301 aataataaaa aacacactgg aggatttttc atg
//
LOCUS       DAZUKR010000335          331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000335 DAZUKR010000000
VERSION     DAZUKR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.351"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>331
                     /locus_tag="ACG89C_002977"
     CDS             <1..>331
                     /locus_tag="ACG89C_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFS4901433.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAZUKR010000336          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000336 DAZUKR010000000
VERSION     DAZUKR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.353"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG89C_002978"
     CDS             complement(<1..>321)
                     /locus_tag="ACG89C_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4901434.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZUKR010000337          315 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000337 DAZUKR010000000
VERSION     DAZUKR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.356"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG89C_002979"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG89C_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS4901435.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           99 a           48 c           50 g          118 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactc
//
LOCUS       DAZUKR010000338          308 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000338 DAZUKR010000000
VERSION     DAZUKR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.357"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACG89C_002980"
     CDS             complement(76..>308)
                     /locus_tag="ACG89C_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4901436.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAZUKR010000339          301 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000339 DAZUKR010000000
VERSION     DAZUKR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.359"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..301
                     /locus_tag="ACG89C_002981"
                     /pseudo
     CDS             1..301
                     /locus_tag="ACG89C_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           60 c           57 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 g
//
LOCUS       DAZUKR010000340          296 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000340 DAZUKR010000000
VERSION     DAZUKR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.360"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>296
                     /locus_tag="ACG89C_002982"
     CDS             <1..>296
                     /locus_tag="ACG89C_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS4901437.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGL"
BASE COUNT          103 a           59 c           58 g           76 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattat
//
LOCUS       DAZUKR010000341          292 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000341 DAZUKR010000000
VERSION     DAZUKR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.361"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACG89C_002983"
     CDS             <1..185
                     /locus_tag="ACG89C_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4901438.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAZUKR010000342          282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000342 DAZUKR010000000
VERSION     DAZUKR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.363"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG89C_002984"
     CDS             <1..>282
                     /locus_tag="ACG89C_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS4901439.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZUKR010000343          270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000343 DAZUKR010000000
VERSION     DAZUKR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.365"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACG89C_002985"
     CDS             <1..>270
                     /locus_tag="ACG89C_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4901440.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAZUKR010000344          261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000344 DAZUKR010000000
VERSION     DAZUKR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.366"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>261
                     /locus_tag="ACG89C_002986"
     CDS             <1..>261
                     /locus_tag="ACG89C_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4901441.1"
                     /translation="LTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGK
                     TALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT           98 a           45 c           49 g           69 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt c
//
LOCUS       DAZUKR010000345          256 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000345 DAZUKR010000000
VERSION     DAZUKR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.367"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           86 a           47 c           35 g           88 t
ORIGIN      
        1 gtatagcaag caaaattctt ctccacttcc agaatttttt attttcattt ttagaaaagt
       61 gaaaaaactg acaacaaaag cttgtttctt tcactctatt aatatcttta gtgaaaaagc
      121 tcgttttata tcgtattctt ccagcgaatg tacttctcat aagtccaaaa aaagctgcga
      181 aaaagaaagt acctcggtac ctactttttc gtagctgcac ataacttgag caagtattaa
      241 tcgaagatac ttttgt
//
LOCUS       DAZUKR010000346          255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000346 DAZUKR010000000
VERSION     DAZUKR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.368"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..>255
                     /locus_tag="ACG89C_002987"
     CDS             95..>255
                     /locus_tag="ACG89C_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4901442.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT           97 a           47 c           40 g           71 t
ORIGIN      
        1 gcgccgattg taaaattaag ctagacaact aaaacagtca tttgtgctac actcaaaata
       61 gttccgacca aagaattata aggagtgagt gcaaataacc tatacctatc ttacaacgga
      121 tgaacttgta ataatagagt cttatttgaa aattaaccaa tctgttgcta aaactgcgca
      181 ttgcttgaat cgttcaagtc aaacgatcca taaagtatat ctgttcttca agcaaagaaa
      241 atcagcctta gaata
//
LOCUS       DAZUKR010000347          239 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000347 DAZUKR010000000
VERSION     DAZUKR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.371"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>239
                     /locus_tag="ACG89C_002988"
     CDS             <1..>239
                     /locus_tag="ACG89C_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4901443.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZUKR010000348          227 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000348 DAZUKR010000000
VERSION     DAZUKR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.372"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="ACG89C_002989"
     rRNA            complement(<1..>227)
                     /locus_tag="ACG89C_002989"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAZUKR010000349          225 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000349 DAZUKR010000000
VERSION     DAZUKR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.373"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           91 a           36 c           29 g           69 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattg
//
LOCUS       DAZUKR010000350          224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000350 DAZUKR010000000
VERSION     DAZUKR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.374"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG89C_002990"
     CDS             <1..>224
                     /locus_tag="ACG89C_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4901444.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZUKR010000351          214 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000351 DAZUKR010000000
VERSION     DAZUKR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.375"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..149
                     /locus_tag="ACG89C_002991"
     CDS             <1..149
                     /locus_tag="ACG89C_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4901445.1"
                     /translation="AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGL
                     IFEN"
BASE COUNT           65 a           46 c           43 g           60 t
ORIGIN      
        1 atgcctttaa ggtgtcccac tcaaacggcg tcactgaggg gctgaataac aagatcaagc
       61 tgattaaacg aattgcgttt ggttatcgca acttttataa ctttcgggca cggatctatt
      121 tacaacaagg tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat
      181 atcggactaa gttttattca aatttgtctt cacc
//
LOCUS       DAZUKR010000352          213 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000352 DAZUKR010000000
VERSION     DAZUKR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.376"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           99 a           22 c           34 g           58 t
ORIGIN      
        1 ggaagatttt aactgtttgc tatagatata tatcatacta gtatcgtaat aaaagtactc
       61 taaaagaaga attttcaaat acgcaaaatt aagtacgaaa gagttgaaac aaatttggag
      121 aaaaatgagc atagaatgtc tgatgagtta aatagaccta aaaattatct aaaagtggag
      181 caaaattagg aattacaaac aaaacactaa att
//
LOCUS       DAZUKR010000353          206 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000353 DAZUKR010000000
VERSION     DAZUKR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.377"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>206)
                     /locus_tag="ACG89C_002992"
     CDS             complement(<1..>206)
                     /locus_tag="ACG89C_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4901446.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DAZUKR010000354          205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000354 DAZUKR010000000
VERSION     DAZUKR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.denovo.378"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>205
                     /locus_tag="ACG89C_002993"
     CDS             <1..>205
                     /locus_tag="ACG89C_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4901447.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           62 a           49 c           38 g           56 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actct
//
LOCUS       DAZUKR010000355        13483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.067, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000355 DAZUKR010000000
VERSION     DAZUKR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.067"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG89C_002994"
     CDS             complement(134..577)
                     /locus_tag="ACG89C_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4901448.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG89C_002995"
     CDS             complement(795..2135)
                     /locus_tag="ACG89C_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4901449.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG89C_002996"
     CDS             2404..3300
                     /locus_tag="ACG89C_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4901450.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG89C_002997"
     CDS             complement(3450..4391)
                     /locus_tag="ACG89C_002997"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4901451.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG89C_002998"
     CDS             4517..5638
                     /locus_tag="ACG89C_002998"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4901452.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG89C_002999"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG89C_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4901453.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG89C_003000"
     CDS             7465..7992
                     /locus_tag="ACG89C_003000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4901454.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACG89C_003001"
     CDS             8203..9264
                     /locus_tag="ACG89C_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4901455.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACG89C_003002"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACG89C_003002"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4901456.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACG89C_003003"
     CDS             10177..12159
                     /locus_tag="ACG89C_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4901457.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG89C_003004"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG89C_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4901458.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..>13483
                     /locus_tag="ACG89C_003005"
     CDS             12881..>13483
                     /locus_tag="ACG89C_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4901459.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPF"
BASE COUNT         4397 a         2446 c         2816 g         3824 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttt
//
LOCUS       DAZUKR010000356        12452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.077, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000356 DAZUKR010000000
VERSION     DAZUKR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.077"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1846
                     /locus_tag="ACG89C_003006"
     CDS             <1..1846
                     /locus_tag="ACG89C_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4901460.1"
                     /translation="TPKRFATRYNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKE
                     QHKFKDVKGKPSRYKMTGSSKDKGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIK
                     RLIEINSIQDVEPDYQFHKEGCSEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFT
                     NVLPKREETTTYHQQKNTILPMFAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRC
                     LEFLERYETQPLQTKKFNEMWLNTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVV
                     SAEVLSRYVFRSDVAYDWNISMDELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSA
                     NDSENRNAYLHELGKITNRSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDF
                     SIRNAFYRVFTKELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTK
                     FLRTLASHYLTELLTSHQFHEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTD
                     LSALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKY
                     AQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(1918..2415)
                     /gene="dfrG"
                     /locus_tag="ACG89C_003007"
     CDS             complement(1918..2415)
                     /gene="dfrG"
                     /locus_tag="ACG89C_003007"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4901461.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            2690..3817
                     /locus_tag="ACG89C_003008"
                     /pseudo
     CDS             2690..3817
                     /locus_tag="ACG89C_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            3915..4103
                     /locus_tag="ACG89C_003009"
     CDS             3915..4103
                     /locus_tag="ACG89C_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS4901462.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4161..4514)
                     /locus_tag="ACG89C_003010"
     CDS             complement(4161..4514)
                     /locus_tag="ACG89C_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901463.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5045..5467
                     /locus_tag="ACG89C_003011"
     CDS             5045..5467
                     /locus_tag="ACG89C_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4901464.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            5464..5694
                     /locus_tag="ACG89C_003012"
     CDS             5464..5694
                     /locus_tag="ACG89C_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4901465.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6192..6392
                     /locus_tag="ACG89C_003013"
     CDS             6192..6392
                     /locus_tag="ACG89C_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS4901466.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            6419..7612
                     /locus_tag="ACG89C_003014"
     CDS             6419..7612
                     /locus_tag="ACG89C_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4901467.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(7680..9245)
                     /gene="guaA"
                     /locus_tag="ACG89C_003015"
     CDS             complement(7680..9245)
                     /gene="guaA"
                     /locus_tag="ACG89C_003015"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4901468.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9304..10227)
                     /gene="coaA"
                     /locus_tag="ACG89C_003016"
     CDS             complement(9304..10227)
                     /gene="coaA"
                     /locus_tag="ACG89C_003016"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4901469.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10423..11028
                     /locus_tag="ACG89C_003017"
     CDS             10423..11028
                     /locus_tag="ACG89C_003017"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4901470.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11336..12352
                     /locus_tag="ACG89C_003018"
     CDS             11336..12352
                     /locus_tag="ACG89C_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4901471.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
BASE COUNT         4108 a         2224 c         2328 g         3792 t
ORIGIN      
        1 aactccaaaa cgatttgcaa caaggtataa caaaatgttg ttcttgccaa cgtcttttaa
       61 atggaacggt tctgaatatc ccattcaata taattactgc atcaatccgt tttgttgtaa
      121 ttttgggaaa gaacagcaca aattcaaaga tgtaaaaggg aaaccaagtc gatataaaat
      181 gactggtagt tccaaagata aaggtcataa aggtatgtac tgcaatgaca atcctattgg
      241 cagaggtgta tcgcaaaact gtacagtaac ccctttatct aattggtctg ttgttgaaga
      301 aattaaacgc cttatagaaa tcaatagcat ccaagatgtt gagccagatt atcaatttca
      361 taaagaaggc tgctcggaag aagaatcaac cccattcaat gagccgaagc aattttacaa
      421 aagggggaaa agtaggggca aatctcaacg ctatcaatgt aaggcgtgta agaagttcac
      481 taatgttcta ccaaaaagag aagaaaccac tacttatcac caacaaaaga acaccattct
      541 tccaatgttc gctaagatgg ttgttggaag ggtttcagtc agtcgtactt gtgatatttt
      601 aggaattgga gttggaacct actatcataa gctggagtgg ctgtataggc gttgtttgga
      661 gtttttagaa cgatatgaga cacaacccct tcaaacaaag aaatttaacg agatgtggct
      721 taatacggac aagatgcact actacctaaa taacgtccgt aagaaaggac aaggttcaaa
      781 gaaatatacg gggtttgaag accttaatat gcaaacgtat atagttgtat ctgctgaggt
      841 cttatcaaga tatgtgttcc gttctgatgt tgcctatgat tggaatattt caatggatga
      901 attaaatgaa gatacaagaa aattcaaaga agaccatctc aatactttta gtcgtaaaaa
      961 tgataggtta gattggtctt attatccaca agaaccatcc gctaacgata gcgaaaatag
     1021 gaatgcttat cttcacgaat tagggaaaat tactaatagg agtagatttg tcgatggttt
     1081 aaatgttgat gctccttata ctacaactgc tcactattgg ttaattaaac aaatggttaa
     1141 tgccgacgaa tggcgaatga ttagtgatga tgacttctct attagaaacg ccttttatag
     1201 agtattcacg aaagaattaa ggttgagcga tgcacatcat tttatttgcc aagttaacaa
     1261 gacgaaaagc agaaaacaat gcttaaaaga attcggacaa gcaaaagcag aactgctcga
     1321 ttggggagat atcagaggat tcaaaacaaa gtttctacgg actttggcat ctcattatct
     1381 aactgaatta ctaacaagtc atcaattcca cgaagaggct attagtaaag atggagaaag
     1441 atatcgcaaa tatgccgata accctatcaa acatccatta gccactaagg ataaaggatt
     1501 ttattcagtt gattgtagaa cggatttatc tgctctggaa ccaaacgaaa tcgcaaagat
     1561 gctattaaat gttaatgacc attcgaccaa tagctttatt caacagataa gacgttatat
     1621 atcatctctt gaaagaccat taacaacagc tcgtggtgat aagaagagct atatttacgc
     1681 taacttcaat ccgaagtatg ctcaatttgc gattacaatt ctaagaactt actacaactt
     1741 ttgtcgccct tttaaatcag cggataaaaa ggttttgaca cctgctcaaa gattagggat
     1801 tacagataaa caatttgatt ggaaagatat aatttatttc aaatgacaaa aagagcttgg
     1861 agtaatccca agctcttgtg ctttatcttt actgttcttc ttcaattaac gcacccgtta
     1921 actcaataag ttttttcttt catatacatg aaaatagtag ttatacggat ttttatcatt
     1981 ctttatccct ttttgggcaa atacctcatt ccattcctca taattcactt ctggaaaaaa
     2041 agtatctcct tcgaattcat gatgtatttt tgtgatgtac attttctcaa cataagggaa
     2101 aaacaaatta taaatctgtt ctcctccgaa aataaaaatt tcttcttcgt ttttacataa
     2161 ctcaaaaaca tcttctattg aatgaacaat ttcacaacca ttaaaggtaa accccttatc
     2221 tctcgtcaga ataatatttc ttctgtcagg taaggctctt ccgattgatt caaggttctt
     2281 cctacctaat attatcggat gtccctttgt agtattttta acatattccc agtccttggg
     2341 aatcctccaa ggaatgtcat tctctttgcc aatcactcta ttcttatcca tcgcagcaat
     2401 caaagaaact ttcatttata acacagcctt tttaactaat ttaccatcta atttcttatt
     2461 tctatcaaaa aaccttttct cattatcctg cccgttagtt gaataagcaa ttttttctat
     2521 aagttatatt atcataatac cacaaaatca caaattttca ttcaattatc attttcaatt
     2581 gcattttata aatcattttg tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc
     2641 cattttgaag tacattttgt cattttgtac tccaaaactg aactacttac ttgcatatat
     2701 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
     2761 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc
     2821 ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg
     2881 agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac
     2941 aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat
     3001 ctcagatagt gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact
     3061 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
     3121 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
     3181 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
     3241 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
     3301 cttaaatcaa tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg
     3361 attatatggt tggaatgtga cggattgtaa aatctgtttt aagtacggtt tatactatag
     3421 ccctgttagt actccagcag attttcggat gcttactcct attgtactgg agcaagcctt
     3481 tagaaaagct ggaacagaat tgttagagcc atatcttagt tttaaagttt atgcaccaca
     3541 ggaatatctt tcacgggcat ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac
     3601 tcaactgaaa aataatgagg tcattattat tggagaaatt cctgctcgat gtattcaaga
     3661 ttatcgcaat gatttaactt tttttacaaa tgggcttagt gtttgtttag cagagctaaa
     3721 aggatatcag gttaccactg gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat
     3781 agataaagta agatatatgt tcaataaaat aacttagtgc gttttatgtt gttatataaa
     3841 tatggtttct tattaaataa gatgaaatat tctttaatat agatttgaat taaagtggaa
     3901 aggaggagat tgttattata aactacaagt ggatattgtg tcctatttgt ggaaataaaa
     3961 caagactacg aatacgagtg gatactatac ttaaaaattt ccctttatac tgccccaaat
     4021 gtaagaacga aactttaatt aatgttcaaa aaatgaatat aataacaatc aaagagccag
     4081 acgccaagac gcagagccga taatttgaga aatgaaactc tcatcttatc ggctcttttt
     4141 gtttatctga attttactga ctagccttca atatttcttg cttcattgat accttttgct
     4201 agtgcttcca ttaaggatag ttctttgtct gtaaagctat ccatgtattt ttctacctgt
     4261 aaccttcgag tgcttttaat caaattatct gtaggtaaga aaaattcatc aatagataca
     4321 tggagtaatg atacaagatc atacagcact tgtgtacttg ggtgttgtcc tttgttttct
     4381 atatttgtta aataacgtgg gtcaatttca atcattgttc ccacttgttc acgagtgaga
     4441 ccccgtttca ttcgagcctc tttaatcgct aaaccaacgg ctctaaaatc gtatttatct
     4501 tcttttttac gcatactaaa tcacctctat acattttact gttcctatat aaccataaac
     4561 aggtatagaa aaacatgtta tatagtttat gagttcctat agaacactgt ggaatttatt
     4621 aatgcgatga atccaatttc aaatagaaag gtcgctgatt ttatttacct atacaacttt
     4681 ataaaattta gtcatactcc tagaaagtag ataaaattgg cgacttaatg gagggaaaag
     4741 tcgccatgag aaagataatc tgtcatctag ttctcttaat taagttctta tcaaaaaaac
     4801 ttatccacct gtactggata gatatgacca tcaatgtggt tttgcagatt acatagatag
     4861 aaaaatcaaa gcatatgaac agagaaaaca atctgtttat gtgctttttt ggtcacttaa
     4921 taaatatctt ttaggatttc tcttcattta tgcaacaaat cccccgttcg tgtcggggtt
     4981 tagtatgaag ggagataaag aagcacatgc cactcccttt cacgacagaa agggggtgaa
     5041 aagcatgaaa ccatcttctt ttcaagaaac catagaaaat cagtttgact atatctgcaa
     5101 acaagttatt gaagatgaac gaaaagatta cttcaaacat ctagacagac tatcagcaaa
     5161 agaagtctcg ttttctgata tagataacta ccttgtcaac tctttttcta ctgttgatac
     5221 gtacgtcact gatttccaac tatttaaatt atttggatta agaatcaatg ttgaaagtga
     5281 tttattaagt gaagccctgc gtcatctttc agagaaaaaa cggaacatta ttttacttca
     5341 ttactttatg gatatgagtg atgtggagat tgcggagtta ctaaatttga atcgttcgac
     5401 tgtttatcgt catcgaataa gtggactagc catgattaaa gaattcatga aggagtgtaa
     5461 agaatgaaaa cagcttatcc aagggttccc ttccccctca ttgtaaaggc gacagatggc
     5521 gacgtggaag caattaatca gattgttaaa cattatcgag ggtatacctc aaaacgttca
     5581 ttacgtcgca tgacagacga atatggaaat tctcatatgg ttattgatga aaccctacgt
     5641 ggcagaatgg aaacgaaact catctctaag attttatcgt tcgaaattaa atagtcccat
     5701 tttttcgtgg aagcgtgtcg aatcgtatcc acgcttcccg atttgggagg tttgttattc
     5761 catcaaagca tattgtgtgt tcagtaggtt ttgataggct aacaagccaa acgttctttg
     5821 acaaccaaat aaaggcaatc gaatacgttt cggtaagaaa agagctgatg aactaacacg
     5881 ccatgaccta caaaattagt gagcgattca tgttagtaat tcgaaagtaa tctttagcag
     5941 gattgctgcc atgactttct tatcgtgata atgatactcc cgtatagcca tagtccgagc
     6001 gttagaagcg tcacaggcaa tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc
     6061 tgtgagcttt gctagagctg ttcgattgct aagatattct tctcttgtca gtgtattatt
     6121 ataaaactat ttgatataat tataggtgaa tattggagtt ttcctagcct ataattggga
     6181 aaggagtaat aatgataacg gtcacaaaaa aggatttagt tgagctagga tatggaactt
     6241 ctttttcagc agacatcata aaaaaagcaa aagaattgat gattacaaaa gggcatatct
     6301 actatcaatc tcgaaagcta gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa
     6361 ttcggttttc aaatgaaaac aggtctgagt agtttttgca cagtaaggag taaaaactat
     6421 ggttaaagac ccaataaaaa aagcaaagaa tggaacctat tattttcgtg caaatctagg
     6481 ttatgattct aaagggaaaa aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga
     6541 agcaagagaa gaatattcga aactgctatt gatgaaacca gaagaattaa gtgagaataa
     6601 ggataaaatg acgtttaaac attatatctt tgaaattttc cttccatggt ataagacaca
     6661 ggtaaagctc agaacatatg aaaatcgctt accaactatt aagaagcatt tctcttattt
     6721 tgataaaatg gctgtttccg atattgagcc tattgatgtg caaaattggc aactaaaatt
     6781 atcaaagaag tgtaagtctt cttatgtaag agctgttcaa ggactatttt cagttgctat
     6841 ggatcgagca attgtccttg ggataaccac cacaaatcca tctaagatta ttggcaatgt
     6901 gaaaaagcaa aagtctaaaa ttgaattttg gacaaaagaa gaatttgaaa aagtaatctc
     6961 tctaatttac aaagaagatt actatcaaca ctttttattt atctctctgt ggtttttatt
     7021 tatgactgga atgcgaattg gtgaagctac tgccattcaa tgggaagata ttgattttga
     7081 ctcaggagta ctaaccatca acaaaacact ctattacaag aatcaatcga attatagatt
     7141 tgttgaacca aaaacaaagg cgagcgttcg tcatattgta ttagataaat gcacattaac
     7201 ttatttatcc gaatggaagg aagtacaaca aaatctaatt cagacagact ttgtaatgag
     7261 ctataacggt attcctaccc aaaaacatac actatcatat gctattgaac gctatgctaa
     7321 gctggctggt atccatcgaa tcagaatcca tgggttacgg cattctcatg cttccttgtt
     7381 aatcagtatg ggagaaaatc cacttatcat taaagatcgt ctaggacacg aagatattga
     7441 gacgacttta ggaacgtatg gacatcttta tcctaacagt aatttcgagg tagctaacaa
     7501 attaaatgga gccatttcct ttaaagaagc aaaacaaaat tatgatactt caccaaagaa
     7561 tcaatttact gtagactact tgcgaaacaa agaaatgaaa aaagtgcaat aaaagtgcag
     7621 taaaaagata agaagagcca gaaacccttg gtataagagg atttctagct ctcctacaac
     7681 tattcccact ccactgttgc tggtggttta gaagtgatgt catacacgat acggttgacg
     7741 tgggctactt cgtttacgat acgtacagag attttttgca agacttccca tgggatacgg
     7801 gcaaagtcag cggtcattcc gtcgattgaa gttacagcac ggataccgac tgtgtagtcg
     7861 taagtacgtc cgtcacccat aacaccgaca gagcggatcc ctggaagaac agtgaaatat
     7921 tgccagatat cacggtcaag tccagcagct gcgatttctt cacgcaagat agcatcagat
     7981 tcgcgtacga tttctagctt gtcttcagtg atttcgccaa gaacacgaat accaagacca
     8041 ggaccaggga atggctgacg ccaaacgatt gaatcaggca tgcctaactg agtccctaat
     8101 tcacggactt cgtctttgaa taatgtgttt aatggttcga tcaattcaaa ttgcatatct
     8161 tctggcagtc cacctacatt atggtgagat ttgatcgtct gtgctgtttc tgtaccagat
     8221 tcgataacgt cagtatacaa cgttccttgt gctaggaaag agacgccttc ttcaccagca
     8281 agttttgttg cttcgtcatc aaaaacatat acgaattcat tcccaatgat tttacgtttt
     8341 tgttctgggt cagatacacc tttcaacttg tctaagaagc gatctcttgc atcgactttg
     8401 atgatgttca agccgaattt tccgcctaga ctttccatta cttgttccgc ttcgcctttg
     8461 cgtagcagac cgtggtcaac aaagatagat gtcaattggt caccgatcgc tttttgtaaa
     8521 agaaccccaa cgacactaga atcaacgcca cctgaaagac caagcaagac ttttttgtca
     8581 ccaacttgtt cacggatttt ttctacttgc atatcgataa agttatccat tgtccagtta
     8641 ccttcacagt gacaaacatc aaaggcaaag tggcgcagca attcgattcc gtattcggaa
     8701 tgacgtactt ctgcatggaa ttggatacca taaaaattac gtgttgtgtc ttggatagaa
     8761 gcaatcggac aatctttgct tgttccgact gtttcaaagc cttcaggaac tttagttacc
     8821 aagtcaccat ggctcatcca tactgtttgt tgttttggtg tttctttaaa caagacagct
     8881 tgatcgcttg ttacttctaa caaggctttc ccatattcgc ggtttttagc aggctctacg
     8941 gaaccgccaa gtttgtacgt gatcaattgc ataccatagc aaatccctaa tacaggaata
     9001 cctaaattga agatctcagg atcgatccca aatgcattct catcatatac actgtttggt
     9061 ccgccagaaa gaatgattcc ctttggattc atcgctttta cttcttctgc agtaatgcgg
     9121 tggctcaata attcggaaaa gactccaaga tcacgaatac gtcttgtgat caattggtta
     9181 tactgactac cgtagtctaa tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt
     9241 gtcaaattta ttacccctat ccctttaaat tttatctcaa aatagtcaaa ctattttaaa
     9301 atgctaatat tttcgcatga aaatcgaatc gatgatatga ttttcagttt tatggagaat
     9361 tatatcggct cttcctcgtg taggcaagat atattcctgt aaatttttca aattcacatt
     9421 tttccataca tcttttgcca ttttaaaggc ttcttcacga tctccgatag caaaacgata
     9481 ataataattc tctggatttt ggaaagccgt atccagtaac gcaccaaaac gatccaaata
     9541 ccatttttcg atcagcacgg gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct
     9601 gacgtagatt tgctgattcg caggtaattg aagcgtattg atcccctcaa cgatcaagat
     9661 atcaggctgc tggatcaatt cgtacttgtc tggaatgata tcatacacat catgtgagta
     9721 aacaggtact tttatctctt cctgaccaga ttttacacgg ttcaagaaat cgatcagcat
     9781 ctccatatca taactttcag gaaatccttt acggtccatg attccacgct cttttaatat
     9841 acgatttgga taaagaaatc cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt
     9901 acgagacaaa atcgtctgca gcagtctggc tgttgtactt tttccaacgg ctacacttcc
     9961 ggcgatcccg ataataaaag gcggtacttc tacgtattta tgaagaaata aacctttact
    10021 caagaacaaa gattcaaatt ctttcatata aagataaatc agatgagcaa gaggtacata
    10081 gatatctcga acatcctgca tggaaatgcg gtcattcaaa cttttgatat tatctagttc
    10141 atcttgtgtc aaaggtgctt gcccattacg ataaaattct cgccattctt ctcttgccac
    10201 acggtagtag ttcattggat cattcatatt agcctcctgc gttctcccat caatggaact
    10261 attttaacac aaaataaacg caaaccccac cacttaagag agattgccga tctttttgta
    10321 taatctttat aatagaaaga aacggagtct tattcttgac ttgcgcacgg atacataaaa
    10381 ggatagactg ttgtcatgaa agaaatgaga aggatggaag acatgacaaa tcattactat
    10441 tctgaacgcc cagatacggc gcacgatttt gaacaatggg cttttgagtt aaaaggcaga
    10501 acctttcaat ttgtaacaga ttctggtgta ttttcacgtg aaacagtgga ttatggttca
    10561 cgtgtcctga ttgatgcttt cgattgggaa gagttacctg atgaaggaaa gatcttagat
    10621 gtgggatgcg ggtatggacc gattgggtta gctttggcat ttgcgacaca gcggtttgtt
    10681 gaaatggtcg acattaatga acgagcggta agtctagcgc aaggaaacgc aaagcgtaac
    10741 caaatcgaac atgttgatat ccatcaatcg aatatctatg aagcagttca tgaagaaacc
    10801 tatgcagcta tcgtaagcaa tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt
    10861 ttgacaggcg catatcctcg gttgaagaag ggcggtacac tgacaattgt gatccagaag
    10921 aaacaaggag cgccgagtgc gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc
    10981 gtcacaaaag ataaaggata ttatatcatc aaaagtgtga aagaataact agctgaaaaa
    11041 tagtggagcg acgaaagagt ggaactgggc gtgcatgtac gggtacgtat cagttctttt
    11101 tttgttgaga agtttttaaa aacaaagaac tattttttct tcaaaaagaa atagtaggaa
    11161 cagagtcgtt gttttttacg ataaaataaa taagatacag aacttgtata gaaggctgcc
    11221 aagtcggtag ataaaggagg tttttatcaa aaaacatact ttcctttttt ctctattctt
    11281 ttcttggggg ttttggtaca ctgataggga atttagatag aagggagcca ataggatgaa
    11341 gctgaccatc agagatatcg cagaaatggc aggcgtatca gtaacaactg tctcacagat
    11401 tttgaataac aaaggaagca gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt
    11461 gaatgaacat cattataagc ccgattattt cgcttcaaat gtgatcaatc gacattcaaa
    11521 gaccatcgga atgatcgtcc ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg
    11581 tgtagagagc tatttgaatc ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag
    11641 ccaagaaaat gagatcaagt atttacaaga gctttcccac cgctcagtgg acggaatctt
    11701 gctggccaca ccaaatattt tacctgagaa atatgcatta gacagcggtt tttatcaaaa
    11761 aatgccaatc attttaatcg atcgcggatt gaatcgtcgg gataacggac ggcttatcgt
    11821 aaaggaatat gagggcgcct atcaagctgt ttcgctgttt attgaaaatg gccatacgaa
    11881 gatcggcatg ctgaaggaaa ccactggata ctatcaatta gaagaacgtt tcaacggata
    11941 tcgtcatgcg ctgaaagatc atggcctgcc ttttaatggg aaatttgttg aacatggaga
    12001 gttgactgtc caaggtggat atactgcatc aaaaaaatta ctcaaacata aagatatcac
    12061 ggctattttt tgcgggaatg atgccatggc gatcggatgc tatcaggcga tcgatgaatt
    12121 gggcaaaaaa atcccagaag atatctcagt cataggattc gatggattaa agctgtcaga
    12181 atacatgatt ccgcaattga cgacagtgca gcagccaagt tttgatattg gattttatgc
    12241 tgcacgtttc ctgatcgata cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt
    12301 cgaaacaaaa ttaattattc gtgagagcgt aaaagtattg acaaagggct aactcggcgg
    12361 tatattgctt atgtaagcat ttacaaaggc tagttagtgc tttttttatt tttagtaaac
    12421 cagtttacta aagtggttta cctttcctaa aa
//
LOCUS       DAZUKR010000357         5614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.150, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000357 DAZUKR010000000
VERSION     DAZUKR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.150"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1027)
                     /locus_tag="ACG89C_003019"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ACG89C_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACG89C_003020"
     CDS             1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACG89C_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4901472.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ACG89C_003021"
     CDS             1852..3006
                     /locus_tag="ACG89C_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS4901473.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ACG89C_003022"
     CDS             3221..4189
                     /locus_tag="ACG89C_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4901474.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ACG89C_003023"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ACG89C_003023"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4901475.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5584
                     /locus_tag="ACG89C_003024"
                     /pseudo
     CDS             5219..5584
                     /locus_tag="ACG89C_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1697 a         1091 c         1263 g         1563 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcggttc
     5581 tgttgcaaag ttttaaataa agaataaaat ccct
//
LOCUS       DAZUKR010000358         4120 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.179, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000358 DAZUKR010000000
VERSION     DAZUKR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.179"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..1548
                     /gene="msr(C)"
                     /locus_tag="ACG89C_003025"
     CDS             70..1548
                     /gene="msr(C)"
                     /locus_tag="ACG89C_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4901476.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1595..2041
                     /locus_tag="ACG89C_003026"
     CDS             1595..2041
                     /locus_tag="ACG89C_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4901477.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2240..2755)
                     /locus_tag="ACG89C_003027"
     CDS             complement(2240..2755)
                     /locus_tag="ACG89C_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4901478.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2872..4074
                     /locus_tag="ACG89C_003028"
     CDS             2872..4074
                     /locus_tag="ACG89C_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4901479.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1227 a          702 c          894 g         1297 t
ORIGIN      
        1 aaacagattt ttctgctctt ttttagtatc gataattgtt gttttacatg aaataggagt
       61 gtaaaacata tggaaaattt agcagtaaat ataacaaatc tacaagtcag ctttggcaac
      121 cagctagaac tatctattga ttctcttcgt gtctatcagc aagatcgtat agggatcata
      181 ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa tagccggtga actttttccc
      241 gatcatggga aaatccaaac agagatcacc ttcaactacc tgcctcaatt aacctatctc
      301 gctgaagcaa aggacctaaa tttggaatta gccagtcatt tccagttaag actggaagaa
      361 acttcagagc ggaaatggag cggaggggaa gaacgaaaga tcgagttgat acgtcttctt
      421 tcttcttatg aacaagggat gcttctagac gagccaacaa cccatctaga tagaaaaagt
      481 attgatcgac tgattgaaga gcttcgttat tattatggta cgctggtttt tgttagtcat
      541 gatcgctatt ttctagatga gctagcatcg aaaatctggg aagtaaaaga cggagaaatc
      601 cgagagtttt cggggaatta tagtgcctat ctcactcaaa aggaattgga gaaaaagact
      661 cagctacgag aagcagagtc gatcatgaaa gagaaaaaac gattggaaaa atcgatccaa
      721 gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca gtaaaaagaa aaagcaacaa
      781 atcagactgg atcggttgtc ttcctctaaa caaaaagaca gtgtacaaaa agccatccaa
      841 aagaatgcga aaacattaga gagaagactc caaaaaatag gagaaacaac caagccgcag
      901 cagatgaaac aaatccgttt tccagtacca aaatctcttg aactccacag ccgctatccc
      961 atcatgggac aaaatgtcca attggaaaga agtgggagaa cattactggt aaatggtgat
     1021 tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg aaaatggttc aggtaagaca
     1081 accttattag aacatatccg caaacaagga gaaggaatcc ttctctctcc gaaagtaagc
     1141 tttcaagtat atcagcaaaa gggttatcaa atgacatctg aagaatccat cattcgtttt
     1201 gtcatgagac aaacagagtt ttcagaatcg cttgtccgta gtttgctgaa tcacttaggg
     1261 tttgctcagg aaactttgac gaaaccgtta tgtacattaa gtgggggaga agcgacccgt
     1321 ctgacgattg ctttgctttt tactaagcca agtaatgtgt tgctgttaga tgaaccgact
     1381 aattttattg atatggcaac gatcgaagct ttagagaagc taatgcaaat atatccggga
     1441 acgattttgt ttacttctca tgattcttac tttgtcgagc gtacggctga tgaagtttat
     1501 gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa atttttaaat gtgctacgat
     1561 gattttcgtg acaaatttaa aggagtggta cagattgcct acaaataaaa aagaaggaat
     1621 aatttttaca acaatgatgt gttttttgat ggtcttgggg atgagcgcgt ataatttatg
     1681 gctgcatcaa gatcttcatt tatcaagttt gttgattggt ctagtgcctg gattcgtggt
     1741 ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg aaaaaaattg cctttaaact
     1801 accagtaaat ccagaaagca aggtccaaat gatcttaact atttcatgct tgatggtttt
     1861 aggaatggtg acatgtatgt ctttattcgg tgtgatcatg gaacaaggaa tacctgatca
     1921 attattctct gcttatatga atgcttggaa aatgaatgtg attgccgctt taccgttgca
     1981 gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa gcaattcaaa gtaatgaatg
     2041 atggaacact gttatttgaa aaaaggcctt ggcatgcttt ttgtcatagt ctcttgaatg
     2101 cagttctaga aaaatagtga tccgaacgat aggttttggc ctgtcgttcg gatcgctttt
     2161 tcttttccaa ctgtattgct cttttattga agataatttg ctcttcaata gatatttcta
     2221 gaaaggttct gtttatccct tactcaaaaa actgctgcat ttgttctctt acttgctgac
     2281 accattggag ttcctgctga caaagcataa ttctgcgctg gatcacaaca taacgtccaa
     2341 attcttgttt cttgtctagc gagaggtcat tcaatttttt ttgatttttt tgcatgattt
     2401 tgttcagctg ttgttctcta gtgaatagca gtccagtagc tgttgtttta tcgagcgtcg
     2461 aaaaagcata gatttttgct agaaattcat ctttttgcgt aggagcggga gtttcctctt
     2521 tgatccattc ctctacaaga gcacgtcctt catcagtcaa gtggtagatc tttttttgag
     2581 agtgctcgtc tttgtccaaa acggtcacat aaccctgctg gtctaacttg gcaagcgttg
     2641 tatagatctg actgtgatgg gcttcccaaa aaattgtgat cagctgcttc aattcatacc
     2701 cagaacgtgg tttggataaa agcacgcata ataatatata ggctaaagta ttcataaaaa
     2761 tcctacctct cgtcttattt tctttattat atcacagtaa agtttccacg ttttacatgt
     2821 gcatattgac atgtaaaata aatcatgtat gatgctaata gagagaaagg gatggaaata
     2881 ttggagaatc aaatagatat acaagaacaa acaaatgaag aaacaaaggg gaaaagttca
     2941 gcgctagttg tagcagctac ctgtatgatc gcttttatgg gagtgggtct agtagaccca
     3001 atcttaaaaa cgattgctgc acagttaaac gctacgccag ctgaaacgac cctgttattt
     3061 actagttaca tgttagtcac tggtgtggtg atgctattta caggtttctt atctagtcgg
     3121 atcggtttga aaaaaacatt gatgacagga ttgtttatca tcgttgtatt cgctggtctt
     3181 ggcggattat ctgggaatat cggaatgctg attgggttac gtgccggctg gggtgtcggg
     3241 aatgctttat ttgtctctac tgctttagca acgatcgtca gtattttagt tggtaacaca
     3301 gaaaaagcaa tcatgatgta tgaagcagca cttggctgcg gaatggctgt tggccctcta
     3361 gtcggtgggg tcttgggcag tggttcatgg cgttatccat tttttggggt agcatttttg
     3421 atgttttgcg gttttatagc cctagcggta cttttacctg aaactgagaa accaaagaga
     3481 aaagtaggcc tttgggaagg tgtcaaagcg ttaagcaatc ataaacttcg ttcagttgga
     3541 ctaatcgcat tgttatataa tttcggattc tttactttac ttgcgtatgc gccattttta
     3601 ttgattggct attctgaatt agaagtcggt tttgtatttt ttggctgggg tgtgcttctg
     3661 gctattgctt cgattttcgt tgcaccggct cttgagcgga agtttacgac ttatcggaca
     3721 attttagcag catttgtctt attcattatt tgtttgctgt tattaggagc tggaacttct
     3781 atgccagtcc ttgtaccgat cagcattgtg ttagcaggat ttttccaagg aattatcaat
     3841 acactgttga cgacgattgc tatggaaata ccaggtctgc aacgaaacgt tgcttcgtcc
     3901 agctatagtt ttgtccgctt tttcggtgga gctttagcac catttattgc aggtgtgatt
     3961 ggtgaacata ttggtgaaag ctatgcgttt tattttgcag gtgccattgt tcttatcagt
     4021 gtatgttgta tcatttatca ccgtaattat tttgctacaa atgaagaaga ataagaaatc
     4081 agaggttgtg aaaaagctgt cctgcatcaa gtcataagaa
//
LOCUS       DAZUKR010000359         3320 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.194, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000359 DAZUKR010000000
VERSION     DAZUKR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.194"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..819
                     /locus_tag="ACG89C_003029"
     CDS             <1..819
                     /locus_tag="ACG89C_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4901480.1"
                     /translation="ECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISK
                     EVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIY
                     RFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEK
                     LVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCT
                     HFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            826..1203
                     /locus_tag="ACG89C_003030"
     CDS             826..1203
                     /locus_tag="ACG89C_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFS4901481.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1357..2139
                     /locus_tag="ACG89C_003031"
     CDS             1357..2139
                     /locus_tag="ACG89C_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS4901482.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2265..2996)
                     /gene="erm(A)"
                     /locus_tag="ACG89C_003032"
     CDS             complement(2265..2996)
                     /gene="erm(A)"
                     /locus_tag="ACG89C_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS4901483.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(3054..3113)
                     /locus_tag="ACG89C_003033"
     CDS             complement(3054..3113)
                     /locus_tag="ACG89C_003033"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS4901484.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
BASE COUNT         1165 a          488 c          619 g         1048 t
ORIGIN      
        1 gaatgtggaa tgaggataag tgaattgtgc accttgaaaa aaggctgtct attagaggac
       61 aaagatggag atttcttttt aaagtattat caatggaaaa tgaaaaagga gcatatagtt
      121 ccaatatcta aagaggtagc tttacttatt aaagttcggg aagataaagt ttcagaggaa
      181 tttccagata gtgaatacct ctttccaaga aaagatggat cgccattaaa acaagaaaca
      241 tttagaggtg agttaaataa attagcttat gagcaaaata tagtggataa atcaggtgag
      301 atttatagat tccatgccca tgcctttcgc catacagtag gaacaagaat gattaacaac
      361 gggatgcccc agcatattgt gcagaaattt ttggggcatg aaagcccaga aatgacaagc
      421 agatacgctc atatctttga tgaaactcta aaaaatgaat ttactaaatt tcaggaaaaa
      481 ctggttacca ataatggaga tgtgcttgat ctagatgaag ataatgaagt cgatgatgta
      541 gagcttcaat ggttcaagaa aaatataaat gcacaagtgc ttccaaatgg ttattgtaga
      601 ttgccagtag tagcaggtgg ttgtccacat gcgaatgcat gcttagattg cactcacttc
      661 tgtaccagta agcaattctt accacagcac gaagaacagt tagagcgtac agaagagtta
      721 ttagccatag ctaaggataa acaatggcaa agacaagtag agactaatag ccgtgttaaa
      781 gagcgtttag aacaaatcat tggaagtttg acggggtaat tatcaatgga taaacaagtt
      841 agaaatacaa cagaaattgt acgtttggcg aagcagaaat caaaaaagac aagggaaaaa
      901 gtagacaaag cgatttctaa attttcgatt gaaggtaaag ttattaattt taattcaata
      961 gcaaaggaag ctaatgtttc taaatcatgg ctttataagg aacacgatat taggcaaaga
     1021 atcgaatccc ttcgtgagcg tcaaataaca gcaaatgtag tctcaaaacc caagaaaagt
     1081 tctcgttcgg aggaaatcct tattaaaacc ttaaaaagaa gagtaatgga attagaaaaa
     1141 gaaaataaaa aattacagaa ccaaattcaa aaattatatg gagatctgta taataaagaa
     1201 taattattaa tctgtagaca aattgtgaaa ggatgtactt aaacgctaac ggtcagcttt
     1261 attgaacagt catttaggta tatgtccaat ctagggtaag taaattgagt atcaatataa
     1321 actttatatg aacataatca gttacgaggt gaaatcatga gcaatttgat taacggaaaa
     1381 ataccaaatc aagcgattca aacattaaaa atcgtaaaag atttatttgg aagttcaata
     1441 gttggagtat atctatttgg ttcagcagta aatggtggtt tacgcattaa cagcgatgta
     1501 gatgttctag tcgtcgtgaa tcatagttta cctcaattaa ctcgaaaaaa actaacagaa
     1561 agactaatga ctatatcagg aaagattgga aatacggatt ctgttagacc acttgaagtt
     1621 acggttataa ataggagtga agttgtccct tggcaatatc ctccaaaaag agaatttata
     1681 tacggtgagt ggctcagggg tgaatttgag aatggacaaa ttcaggaacc aagctatgat
     1741 cctgatttgg ctattgtttt agcacaagca agaaagaata gtatttctct atttggtcct
     1801 gattcttcaa gtatacttgt ctccgtacct ttgacagata ttcgaagagc aattaaggat
     1861 tctttgccag aactaattga ggggataaaa ggtgatgagc gtaatgtaat tttaacccta
     1921 gctcgaatgt ggcaaacagt gactactggt gaaattacct cgaaagatgt cgctgcagaa
     1981 tgggctatac ctcttttacc taaagagcat gtaactttac tggatatagc tagaaaaggc
     2041 tatcggggag agtgtgatga taagtgggaa ggactatatt caaaggtgaa agcactcgtt
     2101 aagtatatga aaaattctat agaaacttct ctcaattagg ctaattttat tgcaataaca
     2161 ggtgcttact tttaaaacta ctgatttatt gataaatatt gaacaatttt tgggaagaat
     2221 aaagcgtcct cttgtgaaat tagagaacgc tttattactt taatttagtg aaacaatttg
     2281 taactattga aaatagaaag aaattgttcc ttcgatagtt tattaatatt agtgacattt
     2341 gcatgcttca aagcctgtcg gaattggttt ttagtgaaaa gaacacgata ttcacggttt
     2401 acccacttat aaacaaaaga tcgatacttt ttgtagtcct tctttgaaat caatggttga
     2461 tgtcgttcaa gaacaatcaa tacagagtct acacttggct taggatgaaa atatagtggt
     2521 ggtacttttt tgagcatttt tatatccatc tccaccatta atagtaaacc caaagctcgt
     2581 tgcagatttt gcaatctttt cgcaaatccc ttctcaacga taagatagct atatttagcc
     2641 tgactttcaa aggtaattct tttgacaata tccgtactga tgttataagg aatattacca
     2701 tatatcttat agtttatatg ttttgggaag gaaaatttta gaatatccgt ttgaatcact
     2761 tttatattct cagaggggtt taccgcttct ttagtcactt gacataagcc tccatcaatt
     2821 tctatagcag taactgatcg actcattttg actagctctt tggtaaaatg tccttttcct
     2881 gatccgattt ctattacgtt gtcttgttta ctgatattcg tgtgattcaa tatttctttt
     2941 acatgctttt tagaagtaat aaaattttgc gtgtctttag ggtttttctg gttcattata
     3001 accttctcct tactggttat aatgaactga cattaagcgt tcattataac caattaattt
     3061 tgatttggtt gataacgaac tttattaata acaaatatag aaaaagtacc catactttta
     3121 tctcctttcc atttttttca ttatgaatga gataaagtaa tttctactac tgcgatactg
     3181 gtgcacataa ttgaaacctc cttatgtaac tgaatatgat tgtatcatct tttcatttta
     3241 gaataaacct ttatatgtaa ttttgcactt gacacttcag acggacgttt tcttcaaaat
     3301 aatatgtttt gaaacttgat
//
LOCUS       DAZUKR010000360         1781 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.237, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000360 DAZUKR010000000
VERSION     DAZUKR010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.237"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..416
                     /locus_tag="ACG89C_003034"
     CDS             24..416
                     /locus_tag="ACG89C_003034"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4901485.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG89C_003035"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG89C_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFS4901486.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAZUKR010000361         1727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.241, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000361 DAZUKR010000000
VERSION     DAZUKR010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.241"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACG89C_003036"
     CDS             complement(<1..283)
                     /locus_tag="ACG89C_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS4901487.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIV"
     gene            376..725
                     /locus_tag="ACG89C_003037"
                     /pseudo
     CDS             376..725
                     /locus_tag="ACG89C_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(997..1287)
                     /locus_tag="ACG89C_003038"
     CDS             complement(997..1287)
                     /locus_tag="ACG89C_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4901488.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1495..>1727)
                     /locus_tag="ACG89C_003039"
     CDS             complement(1495..>1727)
                     /locus_tag="ACG89C_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4901489.1"
                     /translation="SELQNTVNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSS
                     LKQYIQKLEKAKDEVEDTADKSQQIVEEHLRK"
BASE COUNT          523 a          328 c          244 g          632 t
ORIGIN      
        1 ctacaattcc taacataatc gtagaattta ataatttcca tagcataaag tgtttagaaa
       61 agaacattaa gaatagttct ataataattc ttgaactcca tgtcttataa tgttctatcg
      121 aagcacccaa atagccaagt tcatctaatc tacttgcata aactacgtca tccgctctca
      181 tagtcggcat atacatatta aagagtaaaa agatacccca aatccctatt agtaacaaga
      241 tcagttcttt gttattcgtt atttttctat tatgtttgtt catttttata ctccttctta
      301 tatttcctaa tagagtataa catagcttta agcattactt aaagataact taaaagttat
      361 cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta cgatagtcaa
      421 caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta tggattgcag
      481 taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct tttaaaattg
      541 acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta actgtcgaac
      601 caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac tatgataatc
      661 acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac aattccacta
      721 cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat tgagcgaata
      781 ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa atgagtttgg
      841 aaagtttagt gattcatcac aaatacttaa atacatgtag ttatatatag attgaggttc
      901 ttgtgagcgt atgctcgtgt gggttcaaat cccatcgtct gcatccatct ttccaaaaca
      961 agcggctagg acatttttgt ctcagccgct tgttttctag tttattcttt catcttgtcg
     1021 gacctttttt cttgggtata gacttttctc atattctacc acaattttgg gtttccctaa
     1081 cacatgatag gatctcacaa caaattgctt tctattatta taataacctg taacgactaa
     1141 cttcatccct tcgtctactt cagctaggaa gttcaaacta tgggaagcaa tcaaacaatt
     1201 gaccccattt aaactgaaac ggatcaacgg tctggcacta acttgcaaag tacggatctt
     1261 atctactgtt ccatattttt tctccataac cttcacctca ataccattat acgaacatac
     1321 attccctttg taaagaagaa actctgtttt atctttttga ctttttgtct attacctttc
     1381 ttaaaatcat attggcactt cctgcgaaga tgaaggacat gtactaaaaa aaagaggcag
     1441 cagtatagat tactgttgct tctttactaa tttaggttta cttgttaaga aagattattt
     1501 acgcaaatgt tcttcaacaa tttgctgaga tttatcagct gtatcttcca cttcatcttt
     1561 tgctttttct aacttttgaa tatattgttt caagcttgag ttttcagcag ttgcttcgtc
     1621 taatttcttt tgtgtatctg ttaactgagt ttctacattt gttttttctt gtgtcagtgt
     1681 tgcgatttgt tgattcaaac catttacagt attttgtaat tctgaaa
//
LOCUS       DAZUKR010000362         1504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.256, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000362 DAZUKR010000000
VERSION     DAZUKR010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.256"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACG89C_003040"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACG89C_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACG89C_003041"
     CDS             394..477
                     /locus_tag="ACG89C_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS4901490.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG89C_003042"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG89C_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4901491.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZUKR010000363         1382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.267, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000363 DAZUKR010000000
VERSION     DAZUKR010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.267"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1311
                     /locus_tag="ACG89C_003043"
     CDS             <1..1311
                     /locus_tag="ACG89C_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFS4901492.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAZUKR010000364         1350 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.274, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000364 DAZUKR010000000
VERSION     DAZUKR010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.274"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACG89C_003044"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACG89C_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACG89C_003045"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACG89C_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4901493.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAZUKR010000365         1194 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.284, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000365 DAZUKR010000000
VERSION     DAZUKR010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.284"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="ACG89C_003046"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="ACG89C_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS4901494.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DAZUKR010000366          998 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5256
            SAMEA115707602-rid22582913.guided.300, whole genome shotgun
            sequence.
ACCESSION   DAZUKR010000366 DAZUKR010000000
VERSION     DAZUKR010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707602
            Sequence Read Archive: ERR13197330
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288084.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:56:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 31 of 87
            Pseudo Genes (incomplete)         :: 47 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707602-rid22582913.guided.300"
                     /isolate="VRE5256"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..831
                     /gene="aac(6')"
                     /locus_tag="ACG89C_003047"
     CDS             283..831
                     /gene="aac(6')"
                     /locus_tag="ACG89C_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4901495.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          351 a          145 c          208 g          294 t
ORIGIN      
        1 attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat
       61 aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt
      121 tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat
      181 gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt
      241 caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt
      301 gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg
      361 gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc
      421 gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac
      481 ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa
      541 ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg
      601 atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat
      661 gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc
      721 tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa
      781 ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag
      841 gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg
      901 aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac cgtgtaccag
      961 aggttgtgaa aaagtcgctt agtttcgagt agtaagaa
//
